#METABOLOMICS WORKBENCH tetsuya326_20221130_231541 DATATRACK_ID:3616 STUDY_ID:ST002377 ANALYSIS_ID:AN003874 PROJECT_ID:PR001529
VERSION             	1
CREATED_ON             	December 1, 2022, 9:57 am
#PROJECT
PR:PROJECT_TITLE                 	Hepatic Phosphatidylcholine Catabolism Driven by PNPLA7 and PNPLA8 Supplies
PR:PROJECT_TITLE                 	Endogenous Choline to Replenish the Methionine Cycle with Methyl Groups
PR:PROJECT_SUMMARY               	Choline supplies methyl groups for regeneration of methionine and the methyl
PR:PROJECT_SUMMARY               	donor S-adenosylmethionine in the liver. Here we demonstrate that the catabolism
PR:PROJECT_SUMMARY               	of membrane phosphatidylcholine (PC) into water-soluble glycerophosphocholine
PR:PROJECT_SUMMARY               	(GPC) by the phospholipase/lysophospholipase PNPLA8-PNPLA7 axis enables
PR:PROJECT_SUMMARY               	endogenous choline stored in hepatic PC to be utilized in methyl metabolism.
PR:PROJECT_SUMMARY               	PNPLA7-deficient mice show marked decreases in hepatic GPC, choline, and several
PR:PROJECT_SUMMARY               	metabolites related to the methionine cycle, accompanied by various signs of
PR:PROJECT_SUMMARY               	methionine insufficiency including growth retardation, hypoglycemia,
PR:PROJECT_SUMMARY               	hypolipidemia, increased energy consumption, reduced adiposity, increased FGF21,
PR:PROJECT_SUMMARY               	and an altered histone/DNA methylation landscape. Moreover, PNPLA8-deficient
PR:PROJECT_SUMMARY               	mice recapitulate most of these phenotypes. In contrast to wild-type mice fed a
PR:PROJECT_SUMMARY               	methionine/choline-deficient diet, both knockout strains display a decreased
PR:PROJECT_SUMMARY               	hepatic triglyceride likely via reductions of lipogenesis and GPC-derived
PR:PROJECT_SUMMARY               	glycerol flux. Collectively, our findings highlight the biological importance of
PR:PROJECT_SUMMARY               	phospholipid catabolism driven by PNPLA8/PNPLA7 in methyl group flux and
PR:PROJECT_SUMMARY               	triglyceride synthesis in the liver.
PR:INSTITUTE                     	Tokyo Metropolitan Institute of Medical Science
PR:LAST_NAME                     	Hirabayashi
PR:FIRST_NAME                    	Tetsuya
PR:ADDRESS                       	2-6-1 Kamikitazawa, Setagaya-ku, Tokyo, 156-8506, Japan
PR:EMAIL                         	hirabayashi-tt@igakuken.or.jp
PR:PHONE                         	+81-3-5316-3100
#STUDY
ST:STUDY_TITLE                   	Hepatic Phosphatidylcholine Catabolism Driven by PNPLA7 and PNPLA8 Supplies
ST:STUDY_TITLE                   	Endogenous Choline to Replenish the Methionine Cycle with Methyl Groups
ST:STUDY_TITLE                   	(Pnpla7-knockout)
ST:STUDY_SUMMARY                 	Choline supplies methyl groups for regeneration of methionine and the methyl
ST:STUDY_SUMMARY                 	donor S-adenosylmethionine in the liver. Here we demonstrate that the catabolism
ST:STUDY_SUMMARY                 	of membrane phosphatidylcholine (PC) into water-soluble glycerophosphocholine
ST:STUDY_SUMMARY                 	(GPC) by the phospholipase/lysophospholipase PNPLA8-PNPLA7 axis enables
ST:STUDY_SUMMARY                 	endogenous choline stored in hepatic PC to be utilized in methyl metabolism.
ST:STUDY_SUMMARY                 	PNPLA7-deficient mice show marked decreases in hepatic GPC, choline, and several
ST:STUDY_SUMMARY                 	metabolites related to the methionine cycle, accompanied by various signs of
ST:STUDY_SUMMARY                 	methionine insufficiency including growth retardation, hypoglycemia,
ST:STUDY_SUMMARY                 	hypolipidemia, increased energy consumption, reduced adiposity, increased FGF21,
ST:STUDY_SUMMARY                 	and an altered histone/DNA methylation landscape. Moreover, PNPLA8-deficient
ST:STUDY_SUMMARY                 	mice recapitulate most of these phenotypes. In contrast to wild-type mice fed a
ST:STUDY_SUMMARY                 	methionine/choline-deficient diet, both knockout strains display a decreased
ST:STUDY_SUMMARY                 	hepatic triglyceride likely via reductions of lipogenesis and GPC-derived
ST:STUDY_SUMMARY                 	glycerol flux. Collectively, our findings highlight the biological importance of
ST:STUDY_SUMMARY                 	phospholipid catabolism driven by PNPLA8/PNPLA7 in methyl group flux and
ST:STUDY_SUMMARY                 	triglyceride synthesis in the liver.
ST:INSTITUTE                     	Tokyo Metropolitan Institute of Medical Science
ST:LAST_NAME                     	Hirabayashi
ST:FIRST_NAME                    	Tetsuya
ST:ADDRESS                       	2-6-1 Kamikitazawa, Setagaya-ku, Tokyo, 156-8506, Japan
ST:EMAIL                         	hirabayashi-tt@igakuken.or.jp

ST:PHONE                         	+81-3-5316-3100
#SUBJECT
SU:SUBJECT_TYPE                  	Mammal
SU:SUBJECT_SPECIES               	Mus musculus
SU:TAXONOMY_ID                   	10090
SU:GENOTYPE_STRAIN               	C57BL/6N
SU:AGE_OR_AGE_RANGE              	6-weeks old
#FACTORS
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data
SUBJECT_SAMPLE_FACTORS           	-	WT1	Genotype:Wild-type	
SUBJECT_SAMPLE_FACTORS           	-	WT2	Genotype:Wild-type	
SUBJECT_SAMPLE_FACTORS           	-	WT3	Genotype:Wild-type	
SUBJECT_SAMPLE_FACTORS           	-	WT4	Genotype:Wild-type	
SUBJECT_SAMPLE_FACTORS           	-	WT5	Genotype:Wild-type	
SUBJECT_SAMPLE_FACTORS           	-	WT6	Genotype:Wild-type	
SUBJECT_SAMPLE_FACTORS           	-	KO1	Genotype:Pnpla7-knockout	
SUBJECT_SAMPLE_FACTORS           	-	KO2	Genotype:Pnpla7-knockout	
SUBJECT_SAMPLE_FACTORS           	-	KO3	Genotype:Pnpla7-knockout	
SUBJECT_SAMPLE_FACTORS           	-	KO4	Genotype:Pnpla7-knockout	
SUBJECT_SAMPLE_FACTORS           	-	KO5	Genotype:Pnpla7-knockout	
SUBJECT_SAMPLE_FACTORS           	-	KO6	Genotype:Pnpla7-knockout	
#COLLECTION
CO:COLLECTION_SUMMARY            	Mice were deeply anesthetized and perfused intracardially with saline containing
CO:COLLECTION_SUMMARY            	1 mM MOPS (pH 7.4). Liver was rapidly removed, frozen on liquid nitrogen and
CO:COLLECTION_SUMMARY            	stored at –80°C until further processing.
CO:SAMPLE_TYPE                   	Liver
CO:STORAGE_CONDITIONS            	-80℃
#TREATMENT
TR:TREATMENT_SUMMARY             	No treatment in this study.
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	Tissues with internal standards were homogenized with ice-cold methanol and
SP:SAMPLEPREP_SUMMARY            	extracted by the Bligh–Dyer method using chloroform and methanol to separate
SP:SAMPLEPREP_SUMMARY            	and recover the water-soluble fractions. The supernatant was filtered through a
SP:SAMPLEPREP_SUMMARY            	5-kDa cut-off filter (Ultrafree-MC-PLHCC, Human Metabolome Technologies) at
SP:SAMPLEPREP_SUMMARY            	9,100 × g for 2 h at 4 °C, dried in a centrifugal evaporator at 1,500 rpm
SP:SAMPLEPREP_SUMMARY            	at 1,000 Pa, and resuspended in 50 μl of ultrapure water before metabolome
SP:SAMPLEPREP_SUMMARY            	analysis.
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_TYPE           	CE
CH:INSTRUMENT_NAME               	Agilent 7100 CE
CH:COLUMN_NAME                   	Fused silica capillary i.d. 50 μm × 80 cm
#ANALYSIS
AN:ANALYSIS_TYPE                 	MS
#MS
MS:INSTRUMENT_NAME               	Agilent 6490 QQQ
MS:INSTRUMENT_TYPE               	Other
MS:MS_TYPE                       	ESI
MS:ION_MODE                      	NEGATIVE
MS:MS_COMMENTS                   	The spectrometer was scanned from m/z 50 to 1,000. Peaks were extracted using
MS:MS_COMMENTS                   	automatic integration software (MassHunter Quantitative Analysis B.06.00,
MS:MS_COMMENTS                   	Agilent Technologies). Putative metabolites were assigned based on the m/z value
MS:MS_COMMENTS                   	and migration time using Human Metabolomic Technologies’ standard library and
MS:MS_COMMENTS                   	known–unknown peak library. Peak areas were normalized to internal standards
MS:MS_COMMENTS                   	and sample amounts.
#MS_METABOLITE_DATA
MS_METABOLITE_DATA:UNITS	nmol
MS_METABOLITE_DATA_START
Samples	WT1	WT2	WT3	WT4	WT5	KO1	KO2	KO3	KO4	KO5
Factors	Genotype:Wild-type	Genotype:Wild-type	Genotype:Wild-type	Genotype:Wild-type	Genotype:Wild-type	Genotype:Pnpla7-knockout	Genotype:Pnpla7-knockout	Genotype:Pnpla7-knockout	Genotype:Pnpla7-knockout	Genotype:Pnpla7-knockout
2-Hydroxybutyrate	157.61	238.51	149.62	209.49	126.01	426.58	145.14	278.66	217.27	183.06
2-Hydroxyglutarate	42.85	33.82	61.70	57.09	36.48	87.70	31.63	41.36	53.90	24.96
2-Oxobutyrate	68.87	25.83	31.90	76.70	91.05	92.64	37.86	60.34	33.55	109.03
3-(2-Hydroxyphenyl)propionate	13.91	12.31	21.32	23.68	6.16	17.66	4.70	8.19	17.33	7.27
3'-AMP	21.41	10.68	11.38	16.53	20.59	9.82	32.63	23.97	24.39	25.65
3-Hydroxy-3-methylglutarate	2.56	2.94	2.02	1.60	2.07	4.07	2.49	1.18	2.17	1.15
3-Hydroxybutyrate	798.76	1164.59	999.34	1021.46	677.49	981.38	510.99	636.69	756.49	815.49
3-Indoxyl sulfate	2.42	5.22	4.15	5.51	4.25	2.86	2.15	2.78	2.02	3.13
3-Phenyllactate	19.63	16.03	18.39	32.03	20.10	32.23	19.84	25.95	22.69	24.40
3-Ureidopropionate	101.04	154.98	50.55	67.85	74.94	94.21	30.71	16.68	45.59	41.43
3-Phosphoglycerate	44.57	65.55	25.98	46.71	56.20	78.09	34.58	16.46	73.76	26.34
4-Hydroxy-3-methoxymandelate	78.16	94.21	54.50	96.66	83.26	78.49	71.00	82.00	93.34	84.19
4-Methyl-2-oxopentanoate	17.13	10.93	9.18	13.57	24.70	13.14	18.82	19.34	14.24	32.13
4-Oxopentanoate	11.05	10.43	12.14	8.51	9.85	8.64	10.23	10.13	12.32	6.90
5-Oxoproline	503.54	427.83	323.82	390.82	440.38	318.53	347.04	489.47	380.88	547.19
6-Phosphogluconate	136.00	37.62	128.10	157.94	211.75	153.22	264.94	212.11	96.61	247.46
Adenosine 3',5'-diphosphate	4.08	18.90	13.18	44.25	45.78	12.76	N.D.	N.D.	17.08	7.98
Adenylosuccinate	129.98	59.95	225.86	310.14	222.11	160.39	23.02	32.38	64.00	87.57
Adipate	6.52	8.76	10.48	10.81	8.51	10.11	11.65	12.26	8.88	6.16
ADP	193.03	371.53	179.78	243.12	265.52	352.63	154.31	126.18	369.83	138.73
ADP-glucose	25.61	28.44	24.06	23.14	22.05	17.10	14.30	19.58	22.19	14.85
ADP-ribose	4.00	36.47	29.78	36.40	11.84	37.36	13.05	8.43	16.42	5.24
AMP	1666.06	2612.32	2281.56	2168.97	1890.73	3821.44	1076.49	982.74	2570.31	732.80
ATP	5.65	50.05	18.87	22.65	43.43	20.06	3.45	2.69	32.88	11.92
Azelate	2.28	1.97	3.59	2.63	2.54	3.22	6.87	6.01	3.31	2.49
Biotin	73.75	58.88	41.18	38.42	65.14	22.00	36.00	40.58	46.58	54.63
Butanoate	69.29	33.66		25.87	29.90	21.90	N.D.		25.30	41.57
Carbamoylaspartate	2.50	3.14	8.45	6.39	1.33	9.76	N.D.	N.D.	0.87	3.64
CDP	5.26	13.01	2.39	6.51	10.28	8.48	5.83	4.07	13.07	7.21
CDP-choline	91.81	65.82	53.14	61.87	64.08	55.19	45.31	64.91	82.29	73.40
cis-Aconitate	1.86	3.50	5.98	12.65	3.45	2.71	5.72	3.78	1.87	0.75
Citrate	21.43	44.76	127.47	395.65	97.83	39.71	146.92	73.83	17.03	9.48
CMP	113.50	86.60	112.33	139.68	103.37	104.28	131.35	114.56	127.88	166.88
Cysteine sulfinate	10.32	19.77	7.09	17.51	N.D.	20.29	7.47	N.D.	8.12	5.91
DHAP	9.81	7.36	23.51	18.66	21.19	7.67	6.98	23.45	7.51	45.26
Ethanolamine phosphate	1171.91	1047.42	656.58	869.52	1071.20	804.12	987.95	901.84	1128.23	1223.58
F1,6P	213.37	119.48	433.24	134.77	241.57	279.91	60.71	91.24	190.21	110.12
F6P	427.74	88.06	481.60	961.06	372.78	833.07	306.18	565.18	302.34	231.77
FAD	135.63	174.98	116.87	159.23	155.01	123.25	123.81	126.52	153.22	157.43
Fumarate	1231.22	435.21	635.73	943.69	775.46	957.44	555.59	1047.66	623.40	651.03
G1P (D-Glucose 1-phosphate)	401.92	119.39	386.98	608.51	455.55	546.49	286.36	417.07	462.71	272.71
G6P	674.06	128.18	1142.51	2705.05	1095.61	1538.63	821.07	1795.03	690.67	760.48
GDP-mannose	21.41	30.41	21.77	34.46	28.62	41.55	14.57	37.10	35.50	20.23
Glucuronate	35.04	19.44	104.65	214.99	79.76	38.99	164.12	36.78	30.55	67.43
Glutarate	8.94	5.73	9.27	24.89	5.73	28.32	9.12	11.72	6.18	5.57
Glycerophosphate	4848.93	5798.37	2813.15	1716.25	2175.69	6261.09	4835.86	1831.14	5045.21	4585.23
Glycolate	110.32	106.74	119.92	176.21	90.22	153.62	72.89	120.17	99.56	55.65
GMP	370.59	493.92	377.63	428.21	361.08	578.04	237.44	205.33	556.69	142.17
Hippurate	16.57	13.69	52.44	19.29	6.96	42.62	5.22	12.85	9.03	9.27
IDP	28.82	48.19	27.59	33.81	33.89	46.36	19.73	18.36	48.87	21.11
IMP	617.46	552.88	612.48	546.19	755.74	462.15	714.74	228.62	965.02	252.37
Isethionate	32.15	36.43	16.48	27.67	3.72	26.32	74.45	66.51	62.38	61.71
Isocitrate	7.17	7.37	11.47	12.26	10.60	9.86	14.60	13.71	10.14	13.57
Lactate	27378.47	37633.46	32787.13	43985.57	34023.04	33050.32	23462.14	41933.53	28565.39	26826.79
Malate	1977.69	699.76	1554.29	1642.72	1253.77	1345.51	1488.18	2121.55	988.31	1722.73
Malonate	4.39	9.09	10.09	11.64	7.56	3.63	6.82	7.75	6.35	3.68
Mucate	3.20	2.48	2.09	3.03	4.57	2.07	4.79	2.93	3.13	6.11
N-Acetyl-beta-alanine	89.10	54.83	67.79	50.51	64.67	45.65	60.24	60.75	59.14	69.76
N-Acetylaspartate	6.14	5.59	3.95	7.38	3.51	6.96	8.13	7.13	6.46	7.51
N-Acetylglucosamine 1-phosphate	39.49	38.93	23.77	35.18	41.87	31.83	31.62	29.42	31.64	41.87
N-Acetylglucosamine 6-phosphate	31.96	37.87	23.13	29.62	39.08	27.67	36.13	18.93	29.12	34.11
N-Acetylglutamate	405.54	290.96	330.95	376.50	194.52	507.53	144.79	175.74	301.16	175.30
N-Acetylleucine	5.69	6.24	6.62	2.43	2.90	6.28	2.26	5.05	3.61	2.37
N-Acetylmethionine	15.83	18.60	20.13	8.51	7.55	11.95	9.60	16.54	13.88	10.68
N-Acetylneuraminate	31.65	31.88	25.84	31.14	35.67	20.11	23.95	32.27	28.76	35.73
NAD+	70.11	103.61	66.82	78.23	73.42	84.68	82.00	70.39	91.57	76.72
NADP+	41.42	60.73	30.33	40.39	59.01	45.46	33.17	24.51	55.34	41.84
O-Phosphoserine	2.55	1.46	1.05	2.04	2.57	1.14	1.46	1.56	2.03	2.36
Orotate	15.00	9.68	26.34	14.31	N.D.	28.72	6.58	9.62	7.56	
Pantothenate	58.40	38.75	33.64	43.02	51.63	24.72	45.30	58.86	42.46	65.19
PEP(Phosphoenolpyruvate)	10.35	18.53	8.45	16.35	22.50	34.94	15.95	7.27	29.77	8.07
Pimelate	1.15	N.D.	3.23	N.D.	1.26	3.06	5.54	5.45	2.40	1.49
Propionate	104.17	63.98	64.97	123.56	117.82	N.D.	52.82	92.82	53.31	82.00
Pyruvate	N.D.	103.4289432	N.D.	N.D.	78.48542337	N.D.	N.D.	N.D.	N.D.	N.D.
Ribulose 1,5-diphosphate	23.26	21.61	23.27	15.34	20.05	10.28	7.77	7.51	17.97	21.33
Ru5P(D-Ribulose 5-phosphate)	1998.21	2302.20	1223.79	1861.52	1715.22	2295.86	2126.95	1936.64	2605.50	2585.37
S7P(Sedoheptulose 7-phosphate)	93.20	78.15	104.00	247.18	190.84	163.64	168.60	175.97	158.43	127.66
Sebacate	1.19	0.56	0.89	0.66	0.67	1.05	3.63	3.02	1.27	1.24
Succinate	1168.06	801.21	880.36	1188.01	817.30	611.67	741.47	1118.02	664.51	1031.76
Tartrate	7.25	7.73	17.54	14.00	5.37	10.69	1.23	4.94	8.88	2.70
Taurocholate	701.35	128.06	620.13	263.71	327.71	909.42	870.32	151.71	515.42	288.40
Threonate	279.67	206.45	163.76	230.01	191.67	244.40	305.67	191.46	244.82	248.11
trans-4-Hydroxy-3-methoxycinnamate	31.26	23.78	23.99	46.32	27.52	21.59	19.22	25.76	16.30	27.29
trans-Aconitate	10.70	12.96	19.97	19.03	10.87	13.27	1.41	6.92	13.29	4.27
Trehalose 6-phosphate	39.17	62.49	32.16	48.82	38.58	49.74	30.75	35.07	44.71	31.00
UDP	35.18	66.87	29.05	49.66	49.82	51.51	31.80	22.69	70.75	20.35
UDP-glucose	389.50	918.38	293.80	276.13	825.59	258.08	563.68	280.71	999.79	566.04
UDP-glucuronate	1.04	1.40	4.00	9.23	4.83	2.95	6.86	0.82	5.72	1.37
UDP-N-acetylglucosamine	683.99	760.28	637.07	569.08	623.74	581.31	333.00	328.13	520.38	253.81
UMP	561.13	453.43	688.38	817.83	480.54	806.40	437.72	387.11	718.19	253.20
Urate	11.51	20.49	8.81	14.83	16.10	17.72	20.35	62.47	14.81	15.63
XMP(Xanthosine 5'-phosphate)	N.D.	N.D.	N.D.	N.D.	2.23	N.D.	N.D.	N.D.	N.D.	2.53
MS_METABOLITE_DATA_END
#METABOLITES
METABOLITES_START
metabolite_name	KEGG ID	m/z
2-Hydroxybutyrate	C05984	103.0401
2-Hydroxyglutarate	C02630,C01087,C03196	147.0299
2-Oxobutyrate	C00109	101.0244
3-(2-Hydroxyphenyl)propionate	C01198	165.0557
3'-AMP	C01367	346.0558
3-Hydroxy-3-methylglutarate	C03761	161.0455
3-Hydroxybutyrate	C01089,C03197	103.0401
3-Indoxyl sulfate	No ID	212.0023
3-Phenyllactate	No ID	165.0557
3-Ureidopropionate	C02642	131.0462
3-Phosphoglycerate	C00197	184.9857
4-Hydroxy-3-methoxymandelate	No ID	197.0455
4-Methyl-2-oxopentanoate	C00233	129.0557
4-Oxopentanoate	No ID	115.0401
5-Oxoproline	C01879	128.0353
6-Phosphogluconate	C00345	275.0174
Adenosine 3',5'-diphosphate	C00054	426.0221
Adenylosuccinate	C03794	462.0668
Adipate	C06104	145.0506
ADP	C00008	426.0221
ADP-glucose	C00498,C00325	588.0750
ADP-ribose	C00301	558.0644
AMP	C00020	346.0558
ATP	C00002	505.9885
Azelate	No ID	187.0976
Biotin	C00120	243.0809
Butanoate	C00246	87.0452
Carbamoylaspartate	No ID	175.0360
CDP	C00112	402.0109
CDP-choline	C00307	547.1212
cis-Aconitate	C00417	173.0092
Citrate	C00158	191.0197
CMP	C00055	322.0446
Cysteine sulfinate	No ID	152.0023
DHAP	C00111	168.9908
Ethanolamine phosphate	C00346	140.0118
F1,6P	C00354	338.9888
F6P	C05345,C00085	259.0224
FAD	C00016	784.1499
Fumarate	C00122	115.0037
G1P (D-Glucose 1-phosphate)	C00103	259.0224
G6P	C00092	259.0224
GDP-mannose	C00394	604.0699
Glucuronate	C00191	193.0354
Glutarate	C00489	131.0350
Glycerophosphate	C00093	171.0064
Glycolate	C00160	75.0088
GMP	C00144	362.0507
Hippurate	C01586	178.0510
IDP	C00104	427.0062
IMP	C00130	347.0398
Isethionate	C05123	124.9914
Isocitrate	C00311	191.0197
Lactate	C00186,C00256,C01432	89.0244
Malate	C00149,C00497,C00711	133.0142
Malonate	C00383	103.0037
Mucate	No ID	209.0303
N-Acetyl-beta-alanine	No ID	130.0510
N-Acetylaspartate	No ID	174.0408
N-Acetylglucosamine 1-phosphate	C04256	300.0490
N-Acetylglucosamine 6-phosphate	C00357	300.0490
N-Acetylglutamate	C00624	188.0564
N-Acetylleucine	C02710	172.0979
N-Acetylmethionine	C02712	190.0543
N-Acetylneuraminate	C00270	308.0987
NAD+	C00003	662.1019
NADP+	C00006	742.0682
O-Phosphoserine	C01005	184.0017
Orotate	C00295	155.0098
Pantothenate	C00864	218.1034
PEP(Phosphoenolpyruvate)	C00074	166.9751
Pimelate	C02656	159.0663
Propionate	C00163	73.0295
Pyruvate	C00022	87.0088
Ribulose 1,5-diphosphate	C01182	308.9782
Ru5P(D-Ribulose 5-phosphate)	C00199	229.0119
S7P(Sedoheptulose 7-phosphate)	C05382	289.0330
Sebacate	No ID	201.1132
Succinate	C00042	117.0193
Tartrate	C00898	149.0092
Taurocholate	C05122	514.2844
Threonate	C01620
trans-4-Hydroxy-3-methoxycinnamate	No ID	193.0506
trans-Aconitate	C02341	173.0092
Trehalose 6-phosphate	C00689	421.0753
UDP	C00015	402.9949
UDP-glucose	C00029,C00052	565.0478
UDP-glucuronate	C00167	579.0270
UDP-N-acetylglucosamine	C00203,C00043	606.0743
UMP	C00105	323.0286
Urate	C00366	167.0211
XMP(Xanthosine 5'-phosphate)	C00655	363.0347
METABOLITES_END
#END