#METABOLOMICS WORKBENCH Ammons_DRCC_METADATA_031015 DATATRACK_ID:215 STUDY_ID:ST000150 ANALYSIS_ID:AN000237
VERSION 1
CREATED_ON 08-08-2023
#PROJECT
PR:PROJECT_TITLE Association of Metabolic Profile and Microbiome in Chronic Pressure Ulcer Wounds
PR:PROJECT_TYPE NMR metabolomic and 16S rRNA taxonomic profiling in chronic pressure ulcer
PR:PROJECT_TYPE wounds
PR:PROJECT_SUMMARY Chronic, non-healing wounds contribute significantly to the suffering of
PR:PROJECT_SUMMARY patients with co-morbidities in the clinical population with mild to severely
PR:PROJECT_SUMMARY compromised immune systems. Normal wound healing proceeds through a
PR:PROJECT_SUMMARY well-described process. However, in chronic wounds this process seems to become
PR:PROJECT_SUMMARY dysregulated at the transition between resolution of inflammation and
PR:PROJECT_SUMMARY re-epithelialization. Bioburden in the form of colonizing bacteria is a major
PR:PROJECT_SUMMARY contributor to the delayed headlining in chronic wounds such as pressure ulcers.
PR:PROJECT_SUMMARY However how the microbiome influences the wound metabolic landscape is unknown.
PR:PROJECT_SUMMARY Here, we have used a Systems Biology approach to determine the association
PR:PROJECT_SUMMARY between the taxonomic and metabolomic profile of wounds colonized by bacteria.
PR:PROJECT_SUMMARY Pressure ulcer biopsies were harvested from primary chronic wounds and bisected
PR:PROJECT_SUMMARY into top and bottom sections prior to analysis of microbiome by pyrosequencing
PR:PROJECT_SUMMARY and analysis of metabolome using 1H nuclear magnetic resonance (NMR)
PR:PROJECT_SUMMARY spectroscopy. Bacterial taxonomy revealed that wounds were colonized
PR:PROJECT_SUMMARY predominantly by three main phyla, but differed significantly at the genus
PR:PROJECT_SUMMARY level. While taxonomic profiles demonstrated significant variability between
PR:PROJECT_SUMMARY wounds, metabolic profiles shared significant similarity based on the depth of
PR:PROJECT_SUMMARY the wound biopsy. Association between taxonomy and metabolic landscape indicated
PR:PROJECT_SUMMARY significant wound-to-wound similarity in metabolite enrichment sets and
PR:PROJECT_SUMMARY metabolic pathway impacts, especially with regard to amino acid metabolism. To
PR:PROJECT_SUMMARY our knowledge, this is the first demonstration of a statistically robust
PR:PROJECT_SUMMARY correlation between bacterial colonization and metabolic landscape within the
PR:PROJECT_SUMMARY chronic wound environment.
PR:INSTITUTE Montana State University
PR:DEPARTMENT Chemistry and Biochemistry
PR:LABORATORY Ammons and Copie
PR:LAST_NAME Ammons
PR:FIRST_NAME Mary Cloud
PR:ADDRESS 103 CBB, Montana State University, Bozeman, MT 59717
PR:EMAIL mcammons@chemistry.montana.edu
PR:PHONE 406-600-0301
PR:FUNDING_SOURCE NIH 1KO1GM103821-01 and 1RO3AR060995-01A1
PR:DOI http://dx.doi.org/10.21228/M8WW2P
#STUDY
ST:STUDY_TITLE Association of Metabolic Profile and Microbiome in Chronic Pressure Ulcer Wounds
ST:STUDY_SUMMARY Chronic, non-healing wounds contribute significantly to the suffering of
ST:STUDY_SUMMARY patients with co-morbidities in the clinical population with mild to severely
ST:STUDY_SUMMARY compromised immune systems. Normal wound healing proceeds through a
ST:STUDY_SUMMARY well-described process. However, in chronic wounds this process seems to become
ST:STUDY_SUMMARY dysregulated at the transition between resolution of inflammation and
ST:STUDY_SUMMARY re-epithelialization. Bioburden in the form of colonizing bacteria is a major
ST:STUDY_SUMMARY contributor to the delayed headlining in chronic wounds such as pressure ulcers.
ST:STUDY_SUMMARY However how the microbiome influences the wound metabolic landscape is unknown.
ST:STUDY_SUMMARY Here, we have used a Systems Biology approach to determine the association
ST:STUDY_SUMMARY between the taxonomic and metabolomic profile of wounds colonized by bacteria.
ST:STUDY_SUMMARY Pressure ulcer biopsies were harvested from primary chronic wounds and bisected
ST:STUDY_SUMMARY into top and bottom sections prior to analysis of microbiome by pyrosequencing
ST:STUDY_SUMMARY and analysis of metabolome using 1H nuclear magnetic resonance (NMR)
ST:STUDY_SUMMARY spectroscopy. Bacterial taxonomy revealed that wounds were colonized
ST:STUDY_SUMMARY predominantly by three main phyla, but differed significantly at the genus
ST:STUDY_SUMMARY level. While taxonomic profiles demonstrated significant variability between
ST:STUDY_SUMMARY wounds, metabolic profiles shared significant similarity based on the depth of
ST:STUDY_SUMMARY the wound biopsy. Association between taxonomy and metabolic landscape indicated
ST:STUDY_SUMMARY significant wound-to-wound similarity in metabolite enrichment sets and
ST:STUDY_SUMMARY metabolic pathway impacts, especially with regard to amino acid metabolism. To
ST:STUDY_SUMMARY our knowledge, this is the first demonstration of a statistically robust
ST:STUDY_SUMMARY correlation between bacterial colonization and metabolic landscape within the
ST:STUDY_SUMMARY chronic wound environment.
ST:INSTITUTE Montana State University
ST:DEPARTMENT Chemistry and Biochemistry
ST:LABORATORY Ammons
ST:LAST_NAME Ammons
ST:FIRST_NAME Mary Cloud
ST:ADDRESS 103 CBB, Montana State University, Bozeman, MT 59717
ST:EMAIL mcammons@chemistry.montana.edu
ST:PHONE 406-600-0301
ST:SUBMIT_DATE 2015-03-10
#SUBJECT
SU:SUBJECT_TYPE Human
SU:SUBJECT_SPECIES Homo sapiens
SU:TAXONOMY_ID 9606
SU:AGE_OR_AGE_RANGE 63|27|23|43
SU:GENDER Male|Female|Female|Male
SU:HUMAN_RACE African American|African American|White, non-hispanic|African American
SU:HUMAN_ETHNICITY Cerebral palsy, mental retardation, scoliosis, seizure disorder|Deep vein
SU:HUMAN_ETHNICITY thrombosis, anemia, bipolar, herpes simplex virus|Urinary tract infection,
SU:HUMAN_ETHNICITY anxiety, depression
SU:HUMAN_LIFESTYLE_FACTORS None|Cerebral palsy, mental retardation, scoliosis, seizure disorder|Deep vein
SU:HUMAN_LIFESTYLE_FACTORS thrombosis, anemia, bipolar, herpes simplex virus|Urinary tract infection,
SU:HUMAN_LIFESTYLE_FACTORS anxiety, depression
SU:HUMAN_MEDICATIONS Systemic:Bactrim Topical:None|None|Systemic:Vancomycin|Systemic:Doxycycline
SU:HUMAN_MEDICATIONS Topical:Mesalt
SU:HUMAN_NUTRITION Well nourished|G-tube fed, liquid only, malnourished|Well nourished|Inadequate
SU:HUMAN_NUTRITION protein intake
SU:SPECIES_GROUP Human
#SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data
SUBJECT_SAMPLE_FACTORS P1T/P1B P1B Patient:Patient 1
SUBJECT_SAMPLE_FACTORS P1T/P1B P1T Patient:Patient 1
SUBJECT_SAMPLE_FACTORS P2T/P2B P2B Patient:Patient 2
SUBJECT_SAMPLE_FACTORS P2T/P2B P2T Patient:Patient 2
SUBJECT_SAMPLE_FACTORS P3T/P3B P3B Patient:Patient 3
SUBJECT_SAMPLE_FACTORS P3T/P3B P3T Patient:Patient 3
SUBJECT_SAMPLE_FACTORS P4T/P4B P4B Patient:Patient 4
SUBJECT_SAMPLE_FACTORS P4T/P4B P4T Patient:Patient 4
#COLLECTION
CO:COLLECTION_SUMMARY Samples were collected via punch biopsy from wound edge and sectioned into top
CO:COLLECTION_SUMMARY and bottom
CO:COLLECTION_PROTOCOL_COMMENTS Chronicity is defined as wounds lasting more than 30 days
CO:SAMPLE_TYPE Tissue
CO:COLLECTION_METHOD Punch biopsy
CO:COLLECTION_LOCATION Wound Edge
CO:COLLECTION_FREQUENCY Single Collection
CO:VOLUMEORAMOUNT_COLLECTED 9-32 mg
CO:STORAGE_CONDITIONS Flash frozen in liquid nitrogen, shipped on dry ice, stored at -80C
CO:TISSUE_CELL_IDENTIFICATION T=Top section B=Bottom section
CO:TISSUE_CELL_QUANTITY_TAKEN 9-32mg
#TREATMENT
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY Cold methanol/chloroform extraction
SP:PROCESSING_METHOD Biopsy samples were resuspended in ice-cold 60% aqueous methanol and homogenized
SP:PROCESSING_METHOD using a tissue homogenizer (Tissue Tearor™ Model 985370-395, Biospec Products
SP:PROCESSING_METHOD Inc., Bartlesville, OK) set to 2-minute intervals of 10 seconds on and 5 seconds
SP:PROCESSING_METHOD off
SP:PROCESSING_STORAGE_CONDITIONS On ice
SP:EXTRACTION_METHOD 1:1 aqueous chloroform
SP:EXTRACT_CLEANUP Aqueous layers collected and lyophilized
SP:EXTRACT_STORAGE Lyophilized samples were stored at -80C
SP:SAMPLE_RESUSPENSION Resuspended in 10mM NaH2PO4/Na2HPO4
SP:ORGAN Skin
SP:ORGAN_SPECIFICATION Chronic wound biopsy
#CHROMATOGRAPHY
#ANALYSIS
AN:LABORATORY_NAME Copie Lab
AN:ANALYSIS_TYPE NMR
AN:SOFTWARE_VERSION Topspin version 3
AN:OPERATOR_NAME Brian Tripet
AN:DETECTOR_TYPE cryoprobeTM
#NMR
NM:INSTRUMENT_NAME Bruker Avance III
NM:INSTRUMENT_TYPE FT-NMR
NM:NMR_EXPERIMENT_TYPE 1D 1H
NM:SPECTROMETER_FREQUENCY 600 MHz
NM:OFFSET_FREQUENCY 600 mHz
NM:PRESATURATION_POWER_LEVEL cryoprobeTM
NM:CHEMICAL_SHIFT_REF_CPD 10mM NaH2PO4/ Na2HPO4
#NMR_METABOLITE_DATA
NMR_METABOLITE_DATA:UNITS mM/gm tissue
NMR_METABOLITE_DATA_START
Samples P1B P1T P2B P2T P3B P3T P4B P4T
Factors Patient:Patient 1 Patient:Patient 1 Patient:Patient 2 Patient:Patient 2 Patient:Patient 3 Patient:Patient 3 Patient:Patient 4 Patient:Patient 4
1_3-dihydroxyacetone 0.1591
1_3-dimethylurate 0.0548
2-aminobutyrate 0.2581 0.1545 0.3645 0.6474
2-ethylacrylate 0.1548 0.2895
2-hydroxy-3-methylvalerate 0.2864 0.3806 0.2000 0.2810
2-hydroxybutyrate 0.1065 0.4548
2-hydroxyisocaproate 0.2452 0.1524
2-hydroxyphenylacetate 0.0774
2-Octenoate 0.6810
2-oxocaproate 0.1032
2-oxoglutarate 3.7742 0.0818 0.1903
2-phosphoglycerate 1.4682 12.1444 2.9613 1.3333
3_5-dibromotyrosine 0.1387
3-chlorotyrosine 0.1864
3-hydroxyisovalerate 0.2182 0.9667 0.1452 0.1636 0.2710 0.4526 0.1381
3-methyl-2-oxovalerate 0.0839
3-methyladipate 0.3000
3-methylxanthine 0.1516
4-pyridoxate 1.0333 0.2032 0.1136 0.3286
5-aminolevulinate 0.3476
6-hydroxynicotinate 0.1935 0.3381
Acetamide 0.0909
Acetate 1.5955 1.6778 1.5419 1.7727 1.4806 2.2316 1.7048
Acetoacetate 0.0935 0.1000
Acetone 0.2667 0.0591 0.0810
Adenine 0.6091 0.6545 1.0645 1.0368 0.4619
Adenosine 0.1355
Alanine 1.1636 2.1444 1.1065 0.8818 2.0484 2.7263 0.4762
Allantoin 4.5333 0.7484 0.7161 2.0053 1.1857
Alloisoleucine 0.3182 0.8111 0.3909 0.2161 0.9211
Anserine 0.1864 0.3129 0.6048
Arabinitol 0.3194
Arginine 0.3839
Ascorbate 0.9000 0.6682 0.3590 0.8429
Aspartate 0.8548
Betaine 0.1091 0.7333 0.0742 0.0955 0.2632
Butanone 0.1355
Butyrate 0.1258 0.1935
Choline 0.7222 0.2710 0.1364 0.6613 0.8684 0.2143
cis-Aconitate 0.7136 0.2484
Citrate 0.1452
Creatine 0.4955 0.5889 0.2645 0.1500 0.3742 0.2316 0.1667
Creatine phosphate 0.6222 0.2516 0.2000 0.3632 0.1476
Creatinine 0.2742 0.1909 0.3548 0.3316
Cysteine 0.7097
Dimethyl sulfone 0.0545 0.0419
Ethanol 3.6864 8.7111 2.5806 3.0182 2.8774 4.8842 3.1897 3.3857
Ethanolamine 0.6000 0.7484
Ethylene glycol 0.1364 0.1839 0.1452 0.2048
Ethylmalonate 0.2409 0.5000 0.4864 0.6211 0.1762
Formate 1.8591 5.4778 2.8129 2.2091 2.3000 3.3526 2.3286
Fucose 0.1290
Galactarate 0.5258 0.6421
Galactose 1.3387 0.8179
Glucarate 1.6158
Glucose 3.4182 7.3222 0.7677 1.4682 2.5484 2.3263 2.0308 2.6619
Glutamate 2.0955 0.4545 1.7839 2.4895 0.7571
Glutamine 0.8682 0.8032
Glutarate 0.1571
Glycerate 1.5318
Glycine 1.3727 0.7742 0.6318 1.1677 1.2105 0.6429
Glycolate 0.2864 0.8778 0.3387 0.4909 0.1226 0.7053 0.2619
GTP 0.2129
Guanidoacetate 0.2619
Homocysteine 0.2500
Homoserine 0.9682 0.7091 0.5129 0.8286
Hypoxanthine 0.4409 0.1710 0.5273 0.8097 0.7842 0.3476
Inosine 0.2864 0.2226
Isobutyrate 0.0516
Isocaproate 0.1136 0.1579
Isocitrate 0.8323 0.4714
Isoleucine 0.2545
Isovalerate 0.0818
Lactate 3.8000 8.1222 16.0419 7.5227 7.9129 12.7316 2.1590 2.2667
Leucine 0.1727 0.8000 0.0968 0.8194 0.5737 0.1429
Lysine 0.2839
Malate 0.4548
Maleate 0.1258
Malonate 0.1645
Methanol 5.2727 10.0111 3.8032 4.6091 7.1452 7.0000 2.7333 4.4905
Methionine 0.1048
Methylamine 0.1129 0.2000
Methylguanidine 0.1591 0.0806 0.1095
Methylmalonate 0.1591 0.1355 0.3524
myo-inositol 0.8818 0.4387
N-acetylcysteine 0.1419 0.1524
N-acetylglutamate 0.0903
N-acetylglutamine 0.0871
N-acetylglycine 0.1258 0.1048
N-acetylornithine 0.1161
N_N-dimethylformamide 0.0818 0.1789
O-acetylcarnitine 0.1581
O-acetylcholine 0.1387
O-phosphocholine 0.5636 0.4778 0.1871 0.8211
O-phosphoethanolamine 0.8613 1.2789
O-phosphoserine 0.9077
Oxypurinol 4.4182 3.9065 3.0692
Proline 0.8955 0.3762
Propionate 0.1682
Protocatechuate 0.2548
Pyridoxine 0.0548
Pyruvate 0.2581 0.1895 0.1571
Serine 1.0136 1.1452 0.9524
Succinate 0.1682 0.4111 0.0806 0.0636 0.1032
Succinylacetone 0.2381
Tartrate 1.6318 0.8387 1.0387 1.6421 1.0744
Taurine 1.6955 0.6806 0.5000 3.4935 4.2158 0.6048
Threonine 0.9452
Threophylline 0.1136
trans-4-hydroxy-L-proline 0.8667
trans-aconitate 0.1871
Treonate 1.1333
Trimethylamine N-oxide 0.0727 0.6333 0.0387 0.1545 0.0571
Tyramine 0.1452
Tyrosine 0.3818 0.0968
Uridine 0.5409 0.2452
Valerate 0.0742
Valine 0.3545 0.6000 0.5323 0.4636 0.7032 1.3053 0.2095
Valproate 0.0613 0.1591 0.2548 0.2474
Xanthosine 0.6111
Xylose 0.6864
NMR_METABOLITE_DATA_END
#METABOLITES
METABOLITES_START
metabolite_name pubchem_id inchi_key kegg_id other_id other_id_type ri ri_type moverz_quant
1,3-dihydroxyacetone 670 C00184 UMontana_Ammons_Lab_ID
1,3-dimethylurate 70346 UMontana_Ammons_Lab_ID
2-aminobutyrate 6657 UMontana_Ammons_Lab_ID
2-ethylacrylate UMontana_Ammons_Lab_ID
2-hydroxy-3-methylvalerate 10796774 UMontana_Ammons_Lab_ID
2-hydroxybutyrate 11266 C05984 UMontana_Ammons_Lab_ID
2-hydroxyisocaproate 92779 UMontana_Ammons_Lab_ID
2-hydroxyphenylacetate 11914 C01983 UMontana_Ammons_Lab_ID
2-Octenoate UMontana_Ammons_Lab_ID
2-oxocaproate UMontana_Ammons_Lab_ID
2-oxoglutarate 51 C00026 UMontana_Ammons_Lab_ID
2-phosphoglycerate 59 UMontana_Ammons_Lab_ID
3,5-dibromotyrosine 67532 C03224 UMontana_Ammons_Lab_ID
3-chlorotyrosine 119226 UMontana_Ammons_Lab_ID
3-hydroxyisovalerate 69362 UMontana_Ammons_Lab_ID
3-methyl-2-oxovalerate 47 C03465 UMontana_Ammons_Lab_ID
3-methyladipate 6999745 UMontana_Ammons_Lab_ID
3-methylxanthine 70639 C16357 UMontana_Ammons_Lab_ID
4-pyridoxate 6723 C00847 UMontana_Ammons_Lab_ID
5-aminolevulinate 137 C00430 UMontana_Ammons_Lab_ID
6-hydroxynicotinate 72924 C01020 UMontana_Ammons_Lab_ID
Acetamide 178 C06244 UMontana_Ammons_Lab_ID
Acetate 176 C00033 UMontana_Ammons_Lab_ID
Acetoacetate 96 C00164 UMontana_Ammons_Lab_ID
Acetone 180 C00207 UMontana_Ammons_Lab_ID
Adenine 190 C00147 UMontana_Ammons_Lab_ID
Adenosine 60961 C00212 UMontana_Ammons_Lab_ID
Alanine 5950 C00041 UMontana_Ammons_Lab_ID
Allantoin 204 C01551 UMontana_Ammons_Lab_ID
Alloisoleucine 99288 UMontana_Ammons_Lab_ID
Anserine 112072 C01262 UMontana_Ammons_Lab_ID
Arabinitol 827 C01904 UMontana_Ammons_Lab_ID
Arginine 6322 C00062 UMontana_Ammons_Lab_ID
Ascorbate 54670067 C00072 UMontana_Ammons_Lab_ID
Aspartate 5960 C00049 UMontana_Ammons_Lab_ID
Betaine 247 C00719 UMontana_Ammons_Lab_ID
Butanone 6569 C02845 UMontana_Ammons_Lab_ID
Butyrate 20975673 C00246 UMontana_Ammons_Lab_ID
Choline 305 C00114 UMontana_Ammons_Lab_ID
cis-Aconitate 643757 C00417 UMontana_Ammons_Lab_ID
Citrate 311 C00158 UMontana_Ammons_Lab_ID
Creatine 586 C00300 UMontana_Ammons_Lab_ID
Creatine phosphate 587 C02305 UMontana_Ammons_Lab_ID
Creatinine 588 C00791 UMontana_Ammons_Lab_ID
Cysteine 594 C00097 UMontana_Ammons_Lab_ID
Dimethyl sulfone 6213 C11142 UMontana_Ammons_Lab_ID
Ethanol 702 C00469 UMontana_Ammons_Lab_ID
Ethanolamine 700 C00189 UMontana_Ammons_Lab_ID
Ethylene glycol 174 C01380 UMontana_Ammons_Lab_ID
Ethylmalonate 70615 UMontana_Ammons_Lab_ID
Formate 284 C00058 UMontana_Ammons_Lab_ID
Fucose 19466 C01019 UMontana_Ammons_Lab_ID
Galactarate UMontana_Ammons_Lab_ID
Galactose 6036 C00124 UMontana_Ammons_Lab_ID
Glucarate 33037 C00818 UMontana_Ammons_Lab_ID
Glucose 5793 C00031 UMontana_Ammons_Lab_ID
Glutamate UMontana_Ammons_Lab_ID
Glutamine 5961 C00064 UMontana_Ammons_Lab_ID
Glutarate 743 C00489 UMontana_Ammons_Lab_ID
Glycerate 439194 C00258 UMontana_Ammons_Lab_ID
Glycine 750 C00037 UMontana_Ammons_Lab_ID
Glycolate 757 C00160 UMontana_Ammons_Lab_ID
GTP 6830 C00044 UMontana_Ammons_Lab_ID
Guanidoacetate 763 C00581 UMontana_Ammons_Lab_ID
Homocysteine 778 C05330 UMontana_Ammons_Lab_ID
Homoserine 12647 C00263 UMontana_Ammons_Lab_ID
Hypoxanthine 790 C00262 UMontana_Ammons_Lab_ID
Inosine 6021 C00294 UMontana_Ammons_Lab_ID
Isobutyrate 6590 C02632 UMontana_Ammons_Lab_ID
Isocaproate 12587 UMontana_Ammons_Lab_ID
Isocitrate 1198 C00311 UMontana_Ammons_Lab_ID
Isoleucine 6306 C00407 UMontana_Ammons_Lab_ID
Isovalerate 10430 C08262 UMontana_Ammons_Lab_ID
Lactate 107689 C00186 UMontana_Ammons_Lab_ID
Leucine 6106 C00123 UMontana_Ammons_Lab_ID
Lysine 5962 C00047 UMontana_Ammons_Lab_ID
Malate 222656 C00149 UMontana_Ammons_Lab_ID
Maleate 444266 C01384 UMontana_Ammons_Lab_ID
Malonate 867 C00383 UMontana_Ammons_Lab_ID
Methanol 887 C00132 UMontana_Ammons_Lab_ID
Methionine 6137 C00073 UMontana_Ammons_Lab_ID
Methylamine UMontana_Ammons_Lab_ID
Methylguanidine 10111 C02294 UMontana_Ammons_Lab_ID
Methylmalonate 487 C02170 UMontana_Ammons_Lab_ID
myo-inositol 892 C00137 UMontana_Ammons_Lab_ID
N-acetylcysteine 12035 C06809 UMontana_Ammons_Lab_ID
N-acetylglutamate 70914 C00624 UMontana_Ammons_Lab_ID
N-acetylglutamine 25561 UMontana_Ammons_Lab_ID
N-acetylglycine 10972 UMontana_Ammons_Lab_ID
N-acetylornithine 439232 C00437 UMontana_Ammons_Lab_ID
N,N-dimethylformamide 6228 C03134 UMontana_Ammons_Lab_ID
O-acetylcarnitine 1 C02571 UMontana_Ammons_Lab_ID
O-acetylcholine 187 C01996 UMontana_Ammons_Lab_ID
O-phosphocholine 135437 UMontana_Ammons_Lab_ID
O-phosphoethanolamine 1015 C00346 UMontana_Ammons_Lab_ID
O-phosphoserine 68841 C01005 UMontana_Ammons_Lab_ID
Oxypurinol 4644 C07599 UMontana_Ammons_Lab_ID
Proline 145742 C00148 UMontana_Ammons_Lab_ID
Propionate 1032 C00163 UMontana_Ammons_Lab_ID
Protocatechuate 72 C00230 UMontana_Ammons_Lab_ID
Pyridoxine 1054 C00314 UMontana_Ammons_Lab_ID
Pyruvate 1060 C00022 UMontana_Ammons_Lab_ID
Serine 5951 C00065 UMontana_Ammons_Lab_ID
Succinate 1110 C00042 UMontana_Ammons_Lab_ID
Succinylacetone 5312 UMontana_Ammons_Lab_ID
Tartrate 444305 C00898 UMontana_Ammons_Lab_ID
Taurine 1123 C00245 UMontana_Ammons_Lab_ID
Threonine 6288 C00188 UMontana_Ammons_Lab_ID
Threophylline UMontana_Ammons_Lab_ID
trans-4-hydroxy-L-proline 11137200 C04397 UMontana_Ammons_Lab_ID
trans-aconitate 444212 C02341 UMontana_Ammons_Lab_ID
Treonate UMontana_Ammons_Lab_ID
Trimethylamine N-oxide 1145 C01104 UMontana_Ammons_Lab_ID
Tyramine 5610 C00483 UMontana_Ammons_Lab_ID
Tyrosine 6057 C00082 UMontana_Ammons_Lab_ID
Uridine 6029 C00299 UMontana_Ammons_Lab_ID
Valerate 7991 C00803 UMontana_Ammons_Lab_ID
Valine 6287 C00183 UMontana_Ammons_Lab_ID
Valproate UMontana_Ammons_Lab_ID
Xanthosine 64959 C01762 UMontana_Ammons_Lab_ID
Xylose 135191 C00181 UMontana_Ammons_Lab_ID
METABOLITES_END
#END