#METABOLOMICS WORKBENCH METADATA_TEMPLATE.xlsm DATATRACK_ID:245 STUDY_ID:ST000166 ANALYSIS_ID:AN000259 VERSION 1 CREATED_ON 02-08-2024 #PROJECT PR:PROJECT_TITLE Cardiac Resynchronization Therapy Induces Adaptive Metabolic Transitions in the PR:PROJECT_TITLE Metabolomic Profile of Heart Failure PR:PROJECT_SUMMARY Background: Heart failure (HF) is associated with ventricular dyssynchrony and PR:PROJECT_SUMMARY energetic inefficiency, which can be alleviated by cardiac resynchronization PR:PROJECT_SUMMARY therapy (CRT). The aim of this study was to determine the metabolomic signature PR:PROJECT_SUMMARY in HF and its prognostic value for the response to CRT. Methods: This PR:PROJECT_SUMMARY prospective study consisted of 24 patients undergoing CRT for advanced HF and 10 PR:PROJECT_SUMMARY control patients who underwent catheter ablation for supraventricular arrhythmia PR:PROJECT_SUMMARY but not CRT. Blood samples were collected before and 3 months after CRT. PR:PROJECT_SUMMARY Metabolomic profiling of plasma samples was performed using gas PR:PROJECT_SUMMARY chromatography–mass spectrometry and nuclear magnetic resonance. Results: The PR:PROJECT_SUMMARY plasma metabolomic profile was altered in the HF patients, with a distinct panel PR:PROJECT_SUMMARY of metabolites, including Krebs cycle and lipid, amino acid, and nucleotide PR:PROJECT_SUMMARY metabolism. CRT improved the metabolic profile. The succinate/glutamate ratio, PR:PROJECT_SUMMARY an index of Krebs cycle activity, improved from 0.58?0.13 to 2.84?0.60 (P<.05). PR:PROJECT_SUMMARY The glucose/palmitate ratio, an indicator of the balance between glycolytic and PR:PROJECT_SUMMARY fatty acid metabolism, increased from 0.96?0.05 to 1.54?0.09 (P<.01). Compared PR:PROJECT_SUMMARY with the nonresponders to CRT, the responders had a distinct baseline plasma PR:PROJECT_SUMMARY metabolomic profile, including higher isoleucine, phenylalanine, leucine, PR:PROJECT_SUMMARY glucose, and valine levels and lower glutamate levels at baseline (P<.05). PR:PROJECT_SUMMARY Conclusion: CRT improves plasma metabolomic profile of HF patients indicating PR:PROJECT_SUMMARY harmonization of myocardial energy substrate metabolism. CRT responders may have PR:PROJECT_SUMMARY a favorable metabolic profile as a potential biomarker for predicting CRT PR:PROJECT_SUMMARY outcome. PR:INSTITUTE Mayo Clinic PR:DEPARTMENT Department of Medicine PR:LAST_NAME Cha PR:FIRST_NAME Yong-Mei PR:EMAIL ycha@mayo.edu PR:DOI http://dx.doi.org/10.21228/M8C01P #STUDY ST:STUDY_TITLE Cardiac Resynchronization Therapy Induces Adaptive Metabolic Transitions in the ST:STUDY_TITLE Metabolomic Profile of Heart Failure ST:STUDY_TYPE intervention ST:STUDY_SUMMARY This prospective study consisted of 24 patients undergoing CRT for advanced HF ST:STUDY_SUMMARY and 10 control patients who underwent catheter ablation for supraventricular ST:STUDY_SUMMARY arrhythmia but not CRT. Blood samples were collected before and 3 months after ST:STUDY_SUMMARY CRT. Metabolomic profiling of plasma samples was performed using gas ST:STUDY_SUMMARY chromatography–mass spectrometry and nuclear magnetic resonance. ST:INSTITUTE Mayo Clinic ST:DEPARTMENT Department of Medicine ST:LAST_NAME Cha ST:FIRST_NAME Yong-Mei ST:EMAIL ycha@mayo.edu ST:SUBMIT_DATE 2015-05-14 #SUBJECT SU:SUBJECT_TYPE Human SU:SUBJECT_SPECIES Homo sapiens SU:TAXONOMY_ID 9606 SU:SPECIES_GROUP Human #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS - 3M_2 Group:3M | State:Res Obesity (BMI>30=1)=0; Ischemia (1)=1; Age=71; Diabetes (1)=0; HTN (1)=0; CAD (1)=1; Beta (1)=0; Digoxin (1)=1; warfarin (1)=0; ASA (1)=1; Ace (1)=1; Statin (1)=0; EF%=NA; EF < 25=1=NA SUBJECT_SAMPLE_FACTORS - 3M_20 Group:3M | State:Res Obesity (BMI>30=1)=0; Ischemia (1)=1; Age=76; Diabetes (1)=0; HTN (1)=0; CAD (1)=0; Beta (1)=0; Digoxin (1)=0; warfarin (1)=1; ASA (1)=1; Ace (1)=1; Statin (1)=1; EF%=NA; EF < 25=1=NA SUBJECT_SAMPLE_FACTORS - 3M_23 Group:3M | State:Res Obesity (BMI>30=1)=1; Ischemia (1)=0; Age=44; Diabetes (1)=0; HTN (1)=0; CAD (1)=0; Beta (1)=0; Digoxin (1)=0; warfarin (1)=0; ASA (1)=0; Ace (1)=1; Statin (1)=0; EF%=NA; EF < 25=1=NA SUBJECT_SAMPLE_FACTORS - 3M_26 Group:3M | State:Res Obesity (BMI>30=1)=1; Ischemia (1)=0; Age=43; Diabetes (1)=0; HTN (1)=0; CAD (1)=0; Beta (1)=1; Digoxin (1)=0; warfarin (1)=0; ASA (1)=0; Ace (1)=0; Statin (1)=0; EF%=NA; EF < 25=1=NA SUBJECT_SAMPLE_FACTORS - 3M_28 Group:3M | State:Res Obesity (BMI>30=1)=1; Ischemia (1)=0; Age=69; Diabetes (1)=0; HTN (1)=1; CAD (1)=1; Beta (1)=1; Digoxin (1)=0; warfarin (1)=1; ASA (1)=1; Ace (1)=1; Statin (1)=1; EF%=NA; EF < 25=1=NA SUBJECT_SAMPLE_FACTORS - 3M_30 Group:3M | State:Res Obesity (BMI>30=1)=1; Ischemia (1)=1; Age=74; Diabetes (1)=1; HTN (1)=1; CAD (1)=1; Beta (1)=1; Digoxin (1)=0; warfarin (1)=1; ASA (1)=1; Ace (1)=1; Statin (1)=1; EF%=NA; EF < 25=1=NA SUBJECT_SAMPLE_FACTORS - 3M_34 Group:3M | State:Res Obesity (BMI>30=1)=0; Ischemia (1)=1; Age=76; Diabetes (1)=0; HTN (1)=1; CAD (1)=1; Beta (1)=1; Digoxin (1)=0; warfarin (1)=0; ASA (1)=1; Ace (1)=0; Statin (1)=1; EF%=NA; EF < 25=1=NA SUBJECT_SAMPLE_FACTORS - 3M_6 Group:3M | State:Res Obesity (BMI>30=1)=1; Ischemia (1)=1; Age=80; Diabetes (1)=1; HTN (1)=1; CAD (1)=1; Beta (1)=1; Digoxin (1)=1; warfarin (1)=0; ASA (1)=1; Ace (1)=0; Statin (1)=1; EF%=NA; EF < 25=1=NA SUBJECT_SAMPLE_FACTORS - 3M_7 Group:3M | State:Res Obesity (BMI>30=1)=0; Ischemia (1)=0; Age=52; Diabetes (1)=0; HTN (1)=0; CAD (1)=0; Beta (1)=1; Digoxin (1)=0; warfarin (1)=0; ASA (1)=1; Ace (1)=1; Statin (1)=1; EF%=NA; EF < 25=1=NA SUBJECT_SAMPLE_FACTORS - 3M_9 Group:3M | State:Res Obesity (BMI>30=1)=1; Ischemia (1)=0; Age=58; Diabetes (1)=1; HTN (1)=1; CAD (1)=0; Beta (1)=1; Digoxin (1)=1; warfarin (1)=0; ASA (1)=1; Ace (1)=0; Statin (1)=0; EF%=NA; EF < 25=1=NA SUBJECT_SAMPLE_FACTORS - 3M_10 Group:3M | State:Unres Obesity (BMI>30=1)=1; Ischemia (1)=0; Age=47; Diabetes (1)=0; HTN (1)=0; CAD (1)=1; Beta (1)=0; Digoxin (1)=1; warfarin (1)=0; ASA (1)=1; Ace (1)=0; Statin (1)=1; EF%=NA; EF < 25=1=NA SUBJECT_SAMPLE_FACTORS - 3M_14 Group:3M | State:Unres Obesity (BMI>30=1)=0; Ischemia (1)=1; Age=77; Diabetes (1)=0; HTN (1)=1; CAD (1)=0; Beta (1)=1; Digoxin (1)=0; warfarin (1)=0; ASA (1)=1; Ace (1)=0; Statin (1)=0; EF%=NA; EF < 25=1=NA SUBJECT_SAMPLE_FACTORS - 3M_17 Group:3M | State:Unres Obesity (BMI>30=1)=0; Ischemia (1)=1; Age=87; Diabetes (1)=0; HTN (1)=0; CAD (1)=1; Beta (1)=1; Digoxin (1)=0; warfarin (1)=0; ASA (1)=0; Ace (1)=1; Statin (1)=0; EF%=NA; EF < 25=1=NA SUBJECT_SAMPLE_FACTORS - 3M_24 Group:3M | State:Unres Obesity (BMI>30=1)=1; Ischemia (1)=0; Age=47; Diabetes (1)=0; HTN (1)=0; CAD (1)=0; Beta (1)=1; Digoxin (1)=0; warfarin (1)=0; ASA (1)=0; Ace (1)=1; Statin (1)=0; EF%=NA; EF < 25=1=NA SUBJECT_SAMPLE_FACTORS - 3M_27 Group:3M | State:Unres Obesity (BMI>30=1)=0; Ischemia (1)=0; Age=61; Diabetes (1)=0; HTN (1)=1; CAD (1)=0; Beta (1)=0; Digoxin (1)=0; warfarin (1)=0; ASA (1)=1; Ace (1)=1; Statin (1)=1; EF%=NA; EF < 25=1=NA SUBJECT_SAMPLE_FACTORS - 3M_33 Group:3M | State:Unres Obesity (BMI>30=1)=1; Ischemia (1)=0; Age=76; Diabetes (1)=0; HTN (1)=0; CAD (1)=0; Beta (1)=1; Digoxin (1)=0; warfarin (1)=1; ASA (1)=0; Ace (1)=0; Statin (1)=1; EF%=NA; EF < 25=1=NA SUBJECT_SAMPLE_FACTORS - 3M_4 Group:3M | State:Unres Obesity (BMI>30=1)=1; Ischemia (1)=0; Age=82; Diabetes (1)=0; HTN (1)=1; CAD (1)=0; Beta (1)=1; Digoxin (1)=0; warfarin (1)=1; ASA (1)=1; Ace (1)=0; Statin (1)=1; EF%=NA; EF < 25=1=NA SUBJECT_SAMPLE_FACTORS - 3M_5 Group:3M | State:Unres Obesity (BMI>30=1)=1; Ischemia (1)=0; Age=42; Diabetes (1)=1; HTN (1)=0; CAD (1)=0; Beta (1)=1; Digoxin (1)=0; warfarin (1)=1; ASA (1)=0; Ace (1)=0; Statin (1)=1; EF%=NA; EF < 25=1=NA SUBJECT_SAMPLE_FACTORS - 3M_8 Group:3M | State:Unres Obesity (BMI>30=1)=1; Ischemia (1)=0; Age=63; Diabetes (1)=1; HTN (1)=0; CAD (1)=1; Beta (1)=1; Digoxin (1)=1; warfarin (1)=0; ASA (1)=1; Ace (1)=1; Statin (1)=1; EF%=NA; EF < 25=1=NA SUBJECT_SAMPLE_FACTORS - 11_1 Group:Control | State:Control Obesity (BMI>30=1)=0; Ischemia (1)=0; Age=66; Diabetes (1)=0; HTN (1)=1; CAD (1)=1; Beta (1)=1; Digoxin (1)=0; warfarin (1)=1; ASA (1)=1; Ace (1)=0; Statin (1)=0; EF%=65; EF < 25=1=0 SUBJECT_SAMPLE_FACTORS - 12_1 Group:Control | State:Control Obesity (BMI>30=1)=-; Ischemia (1)=0; Age=62; Diabetes (1)=0; HTN (1)=-; CAD (1)=-; Beta (1)=-; Digoxin (1)=-; warfarin (1)=-; ASA (1)=-; Ace (1)=-; Statin (1)=-; EF%=-; EF < 25=1=1 SUBJECT_SAMPLE_FACTORS - 13_1 Group:Control | State:Control Obesity (BMI>30=1)=0; Ischemia (1)=0; Age=65; Diabetes (1)=0; HTN (1)=1; CAD (1)=0; Beta (1)=1; Digoxin (1)=0; warfarin (1)=1; ASA (1)=0; Ace (1)=0; Statin (1)=0; EF%=65; EF < 25=1=0 SUBJECT_SAMPLE_FACTORS - 15_1 Group:Control | State:Control Obesity (BMI>30=1)=0; Ischemia (1)=0; Age=71; Diabetes (1)=0; HTN (1)=0; CAD (1)=0; Beta (1)=1; Digoxin (1)=0; warfarin (1)=1; ASA (1)=0; Ace (1)=0; Statin (1)=0; EF%=64; EF < 25=1=0 SUBJECT_SAMPLE_FACTORS - 16_1 Group:Control | State:Control Obesity (BMI>30=1)=0; Ischemia (1)=0; Age=37; Diabetes (1)=0; HTN (1)=0; CAD (1)=0; Beta (1)=0; Digoxin (1)=0; warfarin (1)=0; ASA (1)=0; Ace (1)=0; Statin (1)=0; EF%=63; EF < 25=1=0 SUBJECT_SAMPLE_FACTORS - 18_1 Group:Control | State:Control Obesity (BMI>30=1)=0; Ischemia (1)=0; Age=83; Diabetes (1)=0; HTN (1)=1; CAD (1)=0; Beta (1)=0; Digoxin (1)=0; warfarin (1)=1; ASA (1)=1; Ace (1)=0; Statin (1)=0; EF%=65; EF < 25=1=0 SUBJECT_SAMPLE_FACTORS - 19_1 Group:Control | State:Control Obesity (BMI>30=1)=1; Ischemia (1)=0; Age=69; Diabetes (1)=0; HTN (1)=0; CAD (1)=0; Beta (1)=0; Digoxin (1)=0; warfarin (1)=1; ASA (1)=1; Ace (1)=0; Statin (1)=0; EF%=66; EF < 25=1=0 SUBJECT_SAMPLE_FACTORS - 21_1 Group:Control | State:Control Obesity (BMI>30=1)=0; Ischemia (1)=0; Age=51; Diabetes (1)=0; HTN (1)=0; CAD (1)=0; Beta (1)=1; Digoxin (1)=0; warfarin (1)=0; ASA (1)=1; Ace (1)=0; Statin (1)=0; EF%=59; EF < 25=1=0 SUBJECT_SAMPLE_FACTORS - 22_1 Group:Control | State:Control Obesity (BMI>30=1)=0; Ischemia (1)=0; Age=72; Diabetes (1)=0; HTN (1)=0; CAD (1)=0; Beta (1)=0; Digoxin (1)=0; warfarin (1)=1; ASA (1)=1; Ace (1)=0; Statin (1)=0; EF%=55; EF < 25=1=0 SUBJECT_SAMPLE_FACTORS - 29_1 Group:Control | State:Control Obesity (BMI>30=1)=0; Ischemia (1)=0; Age=72; Diabetes (1)=0; HTN (1)=1; CAD (1)=0; Beta (1)=0; Digoxin (1)=0; warfarin (1)=1; ASA (1)=1; Ace (1)=1; Statin (1)=1; EF%=63; EF < 25=1=0 SUBJECT_SAMPLE_FACTORS - 2_1 Group:HF | State:Res Obesity (BMI>30=1)=0; Ischemia (1)=1; Age=71; Diabetes (1)=0; HTN (1)=0; CAD (1)=1; Beta (1)=0; Digoxin (1)=1; warfarin (1)=0; ASA (1)=1; Ace (1)=1; Statin (1)=0; EF%=18; EF < 25=1=1 SUBJECT_SAMPLE_FACTORS - 23_1 Group:HF | State:Res Obesity (BMI>30=1)=1; Ischemia (1)=0; Age=44; Diabetes (1)=0; HTN (1)=0; CAD (1)=0; Beta (1)=0; Digoxin (1)=0; warfarin (1)=0; ASA (1)=0; Ace (1)=1; Statin (1)=0; EF%=12; EF < 25=1=1 SUBJECT_SAMPLE_FACTORS - 25_1 Group:HF | State:Res Obesity (BMI>30=1)=1; Ischemia (1)=1; Age=67; Diabetes (1)=1; HTN (1)=1; CAD (1)=1; Beta (1)=1; Digoxin (1)=0; warfarin (1)=1; ASA (1)=1; Ace (1)=1; Statin (1)=1; EF%=20; EF < 25=1=1 SUBJECT_SAMPLE_FACTORS - 26_1 Group:HF | State:Res Obesity (BMI>30=1)=1; Ischemia (1)=0; Age=43; Diabetes (1)=0; HTN (1)=0; CAD (1)=0; Beta (1)=1; Digoxin (1)=0; warfarin (1)=0; ASA (1)=0; Ace (1)=0; Statin (1)=0; EF%=14; EF < 25=1=1 SUBJECT_SAMPLE_FACTORS - 28_1 Group:HF | State:Res Obesity (BMI>30=1)=1; Ischemia (1)=0; Age=69; Diabetes (1)=0; HTN (1)=1; CAD (1)=1; Beta (1)=1; Digoxin (1)=0; warfarin (1)=1; ASA (1)=1; Ace (1)=1; Statin (1)=1; EF%=21; EF < 25=1=1 SUBJECT_SAMPLE_FACTORS - 30_1 Group:HF | State:Res Obesity (BMI>30=1)=1; Ischemia (1)=1; Age=74; Diabetes (1)=1; HTN (1)=1; CAD (1)=1; Beta (1)=1; Digoxin (1)=0; warfarin (1)=1; ASA (1)=1; Ace (1)=1; Statin (1)=1; EF%=20; EF < 25=1=1 SUBJECT_SAMPLE_FACTORS - 3_1 Group:HF | State:Res Obesity (BMI>30=1)=1; Ischemia (1)=0; Age=39; Diabetes (1)=0; HTN (1)=0; CAD (1)=0; Beta (1)=1; Digoxin (1)=1; warfarin (1)=0; ASA (1)=1; Ace (1)=1; Statin (1)=1; EF%=21; EF < 25=1=1 SUBJECT_SAMPLE_FACTORS - 34_1 Group:HF | State:Res Obesity (BMI>30=1)=0; Ischemia (1)=1; Age=76; Diabetes (1)=0; HTN (1)=1; CAD (1)=1; Beta (1)=1; Digoxin (1)=0; warfarin (1)=0; ASA (1)=1; Ace (1)=0; Statin (1)=1; EF%=18; EF < 25=1=1 SUBJECT_SAMPLE_FACTORS - 6_1 Group:HF | State:Res Obesity (BMI>30=1)=1; Ischemia (1)=1; Age=80; Diabetes (1)=1; HTN (1)=1; CAD (1)=1; Beta (1)=1; Digoxin (1)=1; warfarin (1)=0; ASA (1)=1; Ace (1)=0; Statin (1)=1; EF%=15; EF < 25=1=1 SUBJECT_SAMPLE_FACTORS - 7_1 Group:HF | State:Res Obesity (BMI>30=1)=0; Ischemia (1)=0; Age=52; Diabetes (1)=0; HTN (1)=0; CAD (1)=0; Beta (1)=1; Digoxin (1)=0; warfarin (1)=0; ASA (1)=1; Ace (1)=1; Statin (1)=1; EF%=21; EF < 25=1=1 SUBJECT_SAMPLE_FACTORS - 9_1 Group:HF | State:Res Obesity (BMI>30=1)=1; Ischemia (1)=0; Age=58; Diabetes (1)=1; HTN (1)=1; CAD (1)=0; Beta (1)=1; Digoxin (1)=1; warfarin (1)=0; ASA (1)=1; Ace (1)=0; Statin (1)=0; EF%=33; EF < 25=1=0 SUBJECT_SAMPLE_FACTORS - 20_1 Group:HF | State:uc Obesity (BMI>30=1)=0; Ischemia (1)=1; Age=76; Diabetes (1)=0; HTN (1)=0; CAD (1)=0; Beta (1)=0; Digoxin (1)=0; warfarin (1)=1; ASA (1)=1; Ace (1)=1; Statin (1)=1; EF%=45; EF < 25=1=0 SUBJECT_SAMPLE_FACTORS - 32_1 Group:HF | State:uc Obesity (BMI>30=1)=1; Ischemia (1)=1; Age=56; Diabetes (1)=1; HTN (1)=1; CAD (1)=1; Beta (1)=1; Digoxin (1)=0; warfarin (1)=1; ASA (1)=1; Ace (1)=0; Statin (1)=0; EF%=25; EF < 25=1=0 SUBJECT_SAMPLE_FACTORS - 10_1 Group:HF | State:Unres Obesity (BMI>30=1)=1; Ischemia (1)=0; Age=47; Diabetes (1)=0; HTN (1)=0; CAD (1)=1; Beta (1)=0; Digoxin (1)=1; warfarin (1)=0; ASA (1)=1; Ace (1)=0; Statin (1)=1; EF%=30; EF < 25=1=0 SUBJECT_SAMPLE_FACTORS - 1_1 Group:HF | State:Unres Obesity (BMI>30=1)=1; Ischemia (1)=1; Age=76; Diabetes (1)=1; HTN (1)=1; CAD (1)=1; Beta (1)=1; Digoxin (1)=0; warfarin (1)=1; ASA (1)=1; Ace (1)=1; Statin (1)=1; EF%=15; EF < 25=1=1 SUBJECT_SAMPLE_FACTORS - 14_1 Group:HF | State:Unres Obesity (BMI>30=1)=0; Ischemia (1)=1; Age=77; Diabetes (1)=0; HTN (1)=1; CAD (1)=0; Beta (1)=1; Digoxin (1)=0; warfarin (1)=0; ASA (1)=1; Ace (1)=0; Statin (1)=0; EF%=23; EF < 25=1=1 SUBJECT_SAMPLE_FACTORS - 17_1 Group:HF | State:Unres Obesity (BMI>30=1)=0; Ischemia (1)=1; Age=87; Diabetes (1)=0; HTN (1)=0; CAD (1)=1; Beta (1)=1; Digoxin (1)=0; warfarin (1)=0; ASA (1)=0; Ace (1)=1; Statin (1)=0; EF%=25; EF < 25=1=0 SUBJECT_SAMPLE_FACTORS - 24_1 Group:HF | State:Unres Obesity (BMI>30=1)=1; Ischemia (1)=0; Age=47; Diabetes (1)=0; HTN (1)=0; CAD (1)=0; Beta (1)=1; Digoxin (1)=0; warfarin (1)=0; ASA (1)=0; Ace (1)=1; Statin (1)=0; EF%=43; EF < 25=1=0 SUBJECT_SAMPLE_FACTORS - 27_1 Group:HF | State:Unres Obesity (BMI>30=1)=0; Ischemia (1)=0; Age=61; Diabetes (1)=0; HTN (1)=1; CAD (1)=0; Beta (1)=0; Digoxin (1)=0; warfarin (1)=0; ASA (1)=1; Ace (1)=1; Statin (1)=1; EF%=21; EF < 25=1=1 SUBJECT_SAMPLE_FACTORS - 33_1 Group:HF | State:Unres Obesity (BMI>30=1)=1; Ischemia (1)=0; Age=76; Diabetes (1)=0; HTN (1)=0; CAD (1)=0; Beta (1)=1; Digoxin (1)=0; warfarin (1)=1; ASA (1)=0; Ace (1)=0; Statin (1)=1; EF%=30; EF < 25=1=0 SUBJECT_SAMPLE_FACTORS - 4_1 Group:HF | State:Unres Obesity (BMI>30=1)=1; Ischemia (1)=0; Age=82; Diabetes (1)=0; HTN (1)=1; CAD (1)=0; Beta (1)=1; Digoxin (1)=0; warfarin (1)=1; ASA (1)=1; Ace (1)=0; Statin (1)=1; EF%=20; EF < 25=1=1 SUBJECT_SAMPLE_FACTORS - 5_1 Group:HF | State:Unres Obesity (BMI>30=1)=1; Ischemia (1)=0; Age=42; Diabetes (1)=1; HTN (1)=0; CAD (1)=0; Beta (1)=1; Digoxin (1)=0; warfarin (1)=1; ASA (1)=0; Ace (1)=0; Statin (1)=1; EF%=26; EF < 25=1=0 SUBJECT_SAMPLE_FACTORS - 8_1 Group:HF | State:Unres Obesity (BMI>30=1)=1; Ischemia (1)=0; Age=63; Diabetes (1)=1; HTN (1)=0; CAD (1)=1; Beta (1)=1; Digoxin (1)=1; warfarin (1)=0; ASA (1)=1; Ace (1)=1; Statin (1)=1; EF%=25; EF < 25=1=0 SUBJECT_SAMPLE_FACTORS - 8_1A Group:HF | State:Unres Obesity (BMI>30=1)=1; Ischemia (1)=0; Age=63; Diabetes (1)=1; HTN (1)=0; CAD (1)=1; Beta (1)=1; Digoxin (1)=1; warfarin (1)=0; ASA (1)=1; Ace (1)=1; Statin (1)=1; EF%=25; EF < 25=1=0 #COLLECTION CO:COLLECTION_SUMMARY Blood samples were taken from a peripheral vein. These blood samples (5 mL) were CO:COLLECTION_SUMMARY collected into EDTA tubes containing 100 ?L of injected stop solution CO:COLLECTION_SUMMARY (dipyrimidole,10 ?M; EHNA, 5 ?M; iodotubercidin, 2 ?M; AOPCP, 50 ?M, in 0.9% CO:COLLECTION_SUMMARY NaCl) to reduce blood nucleotide metabolism; stored on ice; and then centrifuged CO:COLLECTION_SUMMARY at 3,200 rpm for 10 minutes at 4°C. Plasma aliquots were stored at ?80°C until CO:COLLECTION_SUMMARY analyses. CO:SAMPLE_TYPE Blood #TREATMENT TR:TREATMENT_SUMMARY Heart failure|3-month follow up after cardiac resynchronization therapy TR:TREATMENT_SUMMARY (CRT)|Controls TR:TREATMENT_PROTOCOL_COMMENTS All HF patients underwent a baseline evaluation before CRT, including assessment TR:TREATMENT_PROTOCOL_COMMENTS of New York Heart Association (NYHA) functional class, concomitant TR:TREATMENT_PROTOCOL_COMMENTS cardiovascular conditions (e.g., hypertension, coronary artery disease, and TR:TREATMENT_PROTOCOL_COMMENTS diabetes mellitus), electrocardiographic QRS duration and morphologic TR:TREATMENT_PROTOCOL_COMMENTS characteristics, and transthoracic echocardiography. Echocardiographic TR:TREATMENT_PROTOCOL_COMMENTS parameters included LV end-systolic and diastolic dimensions, LVEF, and TR:TREATMENT_PROTOCOL_COMMENTS pulmonary artery systolic pressure. Medication use was recorded and confirmed TR:TREATMENT_PROTOCOL_COMMENTS that patients were taking optimal medication dose for HF. Patients continued on TR:TREATMENT_PROTOCOL_COMMENTS stable medication dosage during the study.|The HF patients returned for a TR:TREATMENT_PROTOCOL_COMMENTS clinical follow-up 3 months after CRT. The NYHA functional class was reassessed, TR:TREATMENT_PROTOCOL_COMMENTS and echocardiography was repeated.|Ten age-matched control patients underwent TR:TREATMENT_PROTOCOL_COMMENTS catheter ablation for supraventricular arrhythmia with a normal left ventricular TR:TREATMENT_PROTOCOL_COMMENTS ejection fraction (LVEF) of greater than 55%. #SAMPLEPREP SP:SAMPLEPREP_SUMMARY For gas chromatography–mass spectrometry (GC-MS), plasma (100 ?L) was extracted SP:SAMPLEPREP_SUMMARY using a 900-?L methanol:water (8:1, v/v) mixture containing 5 ?g internal SP:SAMPLEPREP_SUMMARY standard, myristic-d27 acid, at ambient temperature 33. Supernatant (900 ?L) was SP:SAMPLEPREP_SUMMARY transferred and completely dried in a vacuum concentrator. Subsequently, the SP:SAMPLEPREP_SUMMARY tubes were methoximated and derivatized and then analyzed using an Agilent 6890 SP:SAMPLEPREP_SUMMARY GC oven with Agilent 5973 MS 34.
Sample Treatment and Instrumental Conditions SP:SAMPLEPREP_SUMMARY for 1H NMR Metabolomic Analysis
For nuclear magnetic resonance (NMR) SP:SAMPLEPREP_SUMMARY imaging, plasma (60 ?L) was diluted 140 ?L with 0.2M phosphate buffer (pH SP:SAMPLEPREP_SUMMARY 7.4):D2O containing a mixture of 16 mM formate and 4 mM TSP (1:1, v/v). After SP:SAMPLEPREP_SUMMARY filtering (0.22 ?m), the samples were transferred into a 3-mm-diameter NMR tube. SP:SAMPLEPREP_SUMMARY High-resolution 1H NMR spectra were acquired at 600 MHz on a Bruker Avance III SP:SAMPLEPREP_SUMMARY 600 spectrometer. Spectra peaks were identified according to Chenomx NMR Suite SP:SAMPLEPREP_SUMMARY 6.1 software and data in the literature 35, 36.
SP:SAMPLEPREP_PROTOCOL_FILENAME Methods-For-Upload.docx #CHROMATOGRAPHY CH:CHROMATOGRAPHY_SUMMARY For gas chromatographymass spectrometry (GC-MS), plasma (100 ?L) was extracted CH:CHROMATOGRAPHY_SUMMARY using a 900-?L methanol:water (8:1, v/v) mixture containing 5 ?g internal CH:CHROMATOGRAPHY_SUMMARY standard, myristic-d27 acid, at ambient temperature 33. Supernatant (900 ?L) was CH:CHROMATOGRAPHY_SUMMARY transferred and completely dried in a vacuum concentrator. Subsequently, the CH:CHROMATOGRAPHY_SUMMARY tubes were methoximated and derivatized and then analyzed using an Agilent 6890 CH:CHROMATOGRAPHY_SUMMARY GC oven with Agilent 5973 MS 34.
Sample Treatment and Instrumental Conditions CH:CHROMATOGRAPHY_SUMMARY for 1H NMR Metabolomic Analysis
For nuclear magnetic resonance (NMR) CH:CHROMATOGRAPHY_SUMMARY imaging, plasma (60 ?L) was diluted 140 ?L with 0.2M phosphate buffer (pH CH:CHROMATOGRAPHY_SUMMARY 7.4):D2O containing a mixture of 16 mM formate and 4 mM TSP (1:1, v/v). After CH:CHROMATOGRAPHY_SUMMARY filtering (0.22 ?m), the samples were transferred into a 3-mm-diameter NMR tube. CH:CHROMATOGRAPHY_SUMMARY High-resolution 1H NMR spectra were acquired at 600 MHz on a Bruker Avance III CH:CHROMATOGRAPHY_SUMMARY 600 spectrometer. Spectra peaks were identified according to Chenomx NMR Suite CH:CHROMATOGRAPHY_SUMMARY 6.1 software and data in the literature 35, 36.
CH:INSTRUMENT_NAME Agilent 6890 GC CH:CHROMATOGRAPHY_TYPE GC #ANALYSIS AN:LABORATORY_NAME Mayo Clinic Metabolomics Core AN:ANALYSIS_TYPE MS AN:ANALYSIS_COMMENTS GC-MS #MS MS:INSTRUMENT_NAME Agilent 5973 MS:INSTRUMENT_TYPE Single quadrupole MS:MS_TYPE EI MS:ION_MODE POSITIVE #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS Peak area MS_METABOLITE_DATA_START Samples 3M_2 3M_20 3M_23 3M_26 3M_28 3M_30 3M_34 3M_6 3M_7 3M_9 3M_10 3M_14 3M_17 3M_24 3M_27 3M_33 3M_4 3M_5 3M_8 11_1 12_1 13_1 15_1 16_1 18_1 19_1 21_1 22_1 29_1 2_1 23_1 25_1 26_1 28_1 30_1 3_1 34_1 6_1 7_1 9_1 20_1 32_1 10_1 1_1 14_1 17_1 24_1 27_1 33_1 4_1 5_1 8_1 8_1A Factors Group:3M | State:Res Group:3M | State:Res Group:3M | State:Res Group:3M | State:Res Group:3M | State:Res Group:3M | State:Res Group:3M | State:Res Group:3M | State:Res Group:3M | State:Res Group:3M | State:Res Group:3M | State:Unres Group:3M | State:Unres Group:3M | State:Unres Group:3M | State:Unres Group:3M | State:Unres Group:3M | State:Unres Group:3M | State:Unres Group:3M | State:Unres Group:3M | State:Unres Group:Control | State:Control Group:Control | State:Control Group:Control | State:Control Group:Control | State:Control Group:Control | State:Control Group:Control | State:Control Group:Control | State:Control Group:Control | State:Control Group:Control | State:Control Group:Control | State:Control Group:HF | State:Res Group:HF | State:Res Group:HF | State:Res Group:HF | State:Res Group:HF | State:Res Group:HF | State:Res Group:HF | State:Res Group:HF | State:Res Group:HF | State:Res Group:HF | State:Res Group:HF | State:Res Group:HF | State:uc Group:HF | State:uc Group:HF | State:Unres Group:HF | State:Unres Group:HF | State:Unres Group:HF | State:Unres Group:HF | State:Unres Group:HF | State:Unres Group:HF | State:Unres Group:HF | State:Unres Group:HF | State:Unres Group:HF | State:Unres Group:HF | State:Unres 2-HB (2-hydroxybutyric acid) 0.3766 0.5248 0.4917 0.3380 0.5304 0.3847 0.2351 0.4828 1.9819 2.1263 0.6662 0.3271 0.2094 0.6143 0.2937 0.4733 0.3986 0.4174 0.4664 1.3473 4.3238 1.0380 2.1264 0.3890 0.6249 1.3016 0.8563 1.0852 1.2712 0.9965 0.6015 1.2090 0.8416 2.9405 1.1482 1.2937 1.5876 1.1172 1.0244 1.2320 0.9391 0.2692 0.7743 0.9992 0.8224 1.1560 1.1581 0.8696 0.8827 1.0049 0.7959 1.7203 1.9136 2-hydroxypyridine 0.7190 1.1716 1.0718 1.2630 1.2288 2.4652 1.6602 1.0393 1.2006 0.9472 0.8550 1.0150 0.7939 1.5166 1.5677 2.1665 0.7777 0.9877 0.9884 0.6046 0.3361 0.8921 0.6067 1.3441 0.7394 0.6493 1.3447 0.8134 0.9194 1.0016 1.1908 0.2197 0.4290 0.6425 0.8507 0.4554 0.7021 0.7554 0.4965 0.8166 1.0392 1.0686 0.5121 0.7768 1.2072 0.9558 1.5494 0.8529 1.7241 0.6194 1.1924 1.1817 1.0752 3-AIB (a-amino[1-(14)C]isobutyric acid) 1.4075 0.4856 0.6095 1.5564 1.2780 1.4054 0.4379 0.7342 1.7727 1.6023 2.5394 0.9647 0.7443 0.8738 1.3842 1.2538 1.3107 1.5651 1.9159 0.3392 1.5518 0.9825 1.1494 0.8827 0.6962 1.1169 0.3179 0.4473 0.3661 0.4166 0.3553 0.5767 0.4247 1.0360 0.9144 1.1068 1.1609 0.5583 0.6242 1.3882 0.4278 0.6885 1.7649 0.6789 0.8538 1.3679 1.1672 1.1139 1.1485 1.5097 1.1241 0.4223 0.4790 3-HB (3-hydroxybutyric acid) 1.1499 0.5836 0.6087 1.4039 1.1441 1.2484 0.6594 0.9124 1.6621 1.4655 2.3938 0.8728 0.7198 0.8805 1.2656 1.0761 1.1477 1.3467 1.6905 0.5479 1.3454 0.8977 1.1421 0.9093 0.7306 1.1372 0.4517 0.6879 0.6738 0.6675 0.5492 0.7331 0.6643 1.0847 0.9610 1.0051 1.1681 0.5915 0.7876 1.2749 0.6313 0.7178 1.6998 0.6343 0.8291 1.2935 1.0230 1.0511 1.0541 1.4056 1.1647 0.6659 0.5875 Acetate 1.9563 0.4411 0.2794 2.1482 1.6576 1.8673 0.2817 0.5428 1.9509 1.6945 2.9076 0.4451 0.6186 0.3900 1.7503 1.8677 1.6966 2.3235 2.2607 0.1532 1.4426 1.3412 1.5850 0.3944 0.3559 0.4474 0.1956 0.9591 0.2670 0.4747 0.2968 0.5582 0.3694 0.5064 1.4149 0.4506 1.5110 0.5044 0.5056 0.4764 1.3682 0.1722 2.3560 0.2017 0.3443 0.7587 1.3599 1.5737 0.4662 1.9040 0.4928 0.3149 0.3978 Acetoacetate 1.6393 0.5711 0.3178 1.8255 1.3536 1.4262 0.5277 0.8010 1.6949 1.3185 2.2888 0.4472 0.5524 0.8587 1.3361 1.4649 1.3653 1.8124 1.8126 0.2314 1.3225 1.0025 1.3633 0.9308 0.5153 0.4531 0.5023 1.0154 0.6320 1.0134 0.6472 0.8671 0.6425 1.0039 1.5595 0.7405 1.3961 0.8289 0.9295 0.4044 1.4201 0.2776 2.1087 0.3080 0.3586 0.7365 1.2339 1.3467 0.4978 1.6211 0.5029 0.5410 0.6318 Acetone 1.1866 0.5184 0.4538 1.6081 1.3804 1.2966 0.4348 0.8365 2.1310 1.2258 2.0608 0.7253 0.4383 1.3976 0.9638 1.1806 1.0930 1.0880 1.8374 0.2387 2.6841 0.6085 0.9903 0.7704 0.2886 0.4913 1.0043 0.4862 0.4282 0.5578 1.0630 0.6078 0.5948 0.9519 1.2850 1.4116 0.8647 0.5548 0.8521 0.5949 1.1152 0.2480 2.1724 0.8976 0.5288 0.8919 1.9185 0.9613 0.6424 1.1468 0.6442 1.2042 1.4426 Acetylsalicylic acid 7.9157 0.3946 0.0563 0.0563 0.0563 1.4313 1.9134 0.5581 0.0563 0.0563 2.9238 3.3865 0.0563 0.0563 2.0631 0.0563 0.3586 0.0563 0.4297 0.0563 0.0563 0.0563 0.0563 0.0563 0.0563 0.0563 0.0563 0.0563 0.7502 2.6737 0.0563 1.4037 0.0563 0.8862 0.0563 0.4688 0.6254 0.0563 10.2738 0.0563 0.0563 0.0563 0.3707 0.0563 1.5006 0.0563 0.0563 8.7164 0.0563 0.1470 0.0563 1.0659 1.0548 Alanine 1.6791 0.3373 0.6374 1.9654 1.4901 1.7309 0.2660 0.3733 1.7514 1.6697 2.7113 0.8329 0.3559 1.0106 1.4326 1.5630 1.4001 1.8175 1.9996 0.2021 1.4119 1.3177 1.5168 1.5217 0.7734 0.7348 0.5651 0.8620 0.2672 0.3200 0.4589 0.2098 0.2700 0.7188 1.3340 0.9853 1.2298 0.2788 0.4079 0.7280 1.2092 0.5452 2.1567 0.6237 0.6312 0.8254 1.2500 1.3924 0.7626 1.5705 0.2426 0.1740 0.4784 Allo-inositol 2.8699 0.6397 1.0588 0.5691 1.0544 2.1045 0.5842 0.9738 0.7471 1.0487 1.0823 1.1200 3.8480 0.7202 0.7409 0.6634 2.6340 1.3300 0.7228 0.1765 0.9847 0.2251 0.2307 0.4131 0.7462 0.6850 0.2879 0.6577 0.7399 2.4510 0.3752 0.8336 0.3628 0.4490 1.2002 0.1952 0.7240 0.6295 0.2630 1.1588 0.5933 0.4848 0.3534 1.2049 1.1390 2.4804 0.3164 0.2203 0.7542 3.7803 2.4582 0.4538 0.4600 Arabitol 2.1645 0.6355 0.8831 0.6685 0.7230 1.7177 0.3116 0.7334 0.5414 0.8016 1.4279 1.7013 4.6295 0.3861 0.5460 0.6502 3.2225 1.8213 0.7136 0.6103 0.3671 0.6404 0.0607 0.2240 1.0880 0.4518 0.1876 0.7391 0.9270 3.4015 1.0263 1.2001 0.3983 0.6794 0.9883 0.3538 0.3984 0.9206 0.5618 0.5618 0.2284 0.4351 0.9341 1.0536 0.7997 1.8379 0.4789 0.3996 0.6490 3.1009 2.0259 0.4237 0.5682 Benzoic acid 0.0723 0.3824 0.7236 0.6049 0.7401 0.6249 0.9155 0.5734 0.8493 1.0517 0.3711 0.7868 0.0723 0.2500 0.7217 0.2831 0.0723 0.0723 0.5960 0.5235 0.3669 1.1958 0.6271 1.8138 2.5097 5.2777 0.5908 1.2986 0.7096 0.0723 2.7820 1.2902 0.8593 1.7506 1.0975 0.4589 0.4151 0.6821 0.7631 1.1723 0.0723 0.7748 1.3887 0.1682 1.0964 0.5413 1.0306 1.1560 0.7201 0.0723 8.3276 0.7992 0.8315 Beta- alanine 1.3355 0.9723 1.6175 1.7313 1.2039 2.0666 1.0164 1.0173 0.2962 1.5175 0.9605 1.3696 1.2830 0.4401 1.4681 0.6448 1.0151 0.7523 0.8518 1.6547 0.5513 1.5143 0.9154 0.9537 1.1840 0.5222 0.9059 0.4573 0.8029 0.3721 1.8453 0.4391 0.6408 0.7251 0.7172 1.1356 0.5328 0.7118 0.5595 0.7918 1.2711 1.2110 0.8443 0.7668 1.4055 0.9702 0.9527 0.6680 1.2109 0.7289 1.4265 1.1435 0.9083 Cholesterol 0.8131 0.8360 1.4538 1.0045 1.2966 0.8862 0.5743 0.9590 0.9870 1.7598 0.8752 1.4124 0.6484 1.0680 1.3453 0.7723 1.3169 0.7660 1.1356 1.2996 1.4832 1.4936 0.7766 1.9901 0.7771 1.0591 0.8875 1.1723 0.5720 0.6836 1.0952 0.6949 0.9946 0.9935 0.8382 1.1062 0.5687 0.7612 0.9509 1.2088 0.6617 1.1017 0.9478 0.5633 1.5684 0.6196 1.0972 1.2122 0.7391 0.8822 0.6423 0.8239 0.8232 Citrate 1.9075 0.5469 0.4043 1.9641 1.4019 1.8349 0.3130 1.1742 1.7993 1.3684 2.8028 0.8119 1.4834 0.3806 1.5207 1.5399 1.5158 2.3296 2.2747 0.1129 1.1430 0.9526 1.0716 0.0155 0.8250 0.8511 0.2822 0.2755 0.3331 0.6865 0.0155 0.7664 0.6368 0.8283 0.9063 0.4637 1.3637 0.8200 0.7106 1.3650 0.4989 0.0155 1.8034 0.1968 0.2586 1.6840 0.9597 1.0264 1.0338 1.5886 1.2550 0.4174 0.4628 Creatine 1.6491 0.8082 0.5693 1.5076 1.1146 1.2886 0.7890 1.2045 1.3412 1.2957 2.0558 0.7411 1.0732 0.8719 1.0384 1.3236 1.3569 1.8494 1.6279 0.3663 1.0048 0.8746 1.0654 0.9539 0.7389 0.6236 0.5521 0.7252 0.6432 0.9171 0.7523 0.9376 0.8474 0.7539 0.9491 0.7571 1.1050 1.0794 0.9348 0.6841 1.3206 0.4078 1.6109 0.5897 0.5953 1.1316 0.8636 1.0453 0.8951 1.4659 0.9671 0.6016 0.7338 Creatinine 1.6439 0.7512 0.4320 1.6755 1.2650 1.5069 0.6586 1.2050 1.5942 1.3121 2.4017 0.6167 0.8397 0.7849 1.1533 1.5307 1.4335 2.0574 1.9026 0.2001 0.9774 0.8724 1.0820 0.7835 0.7091 0.5806 0.4855 0.6696 0.5378 0.7762 0.5533 0.8356 0.7856 0.7742 1.0184 0.7294 1.2831 1.0062 0.9235 0.7214 1.4571 0.2123 1.7516 0.3793 0.4012 1.0625 0.9151 1.1935 1.0185 1.4963 0.8388 0.5535 0.6505 Creatinine g 2.0583 0.5604 0.9699 0.7701 0.5757 0.6539 0.7301 0.6523 0.4459 1.7433 0.9300 1.1758 2.4685 0.8634 0.7314 0.1484 2.2421 1.8819 0.5799 0.6194 1.1592 0.9922 0.6675 0.4147 0.6484 0.1484 0.1484 0.4601 0.5412 3.5191 1.4891 1.7289 0.7173 0.9272 0.1484 1.3993 0.5001 0.6143 0.8511 0.3944 1.0364 0.4970 1.8403 1.4621 1.0078 2.5799 0.5892 0.5937 0.4826 2.3651 1.5503 0.5771 0.1484 Cysteine 1.5021 0.9242 0.9816 1.2223 1.5239 0.9478 0.3603 0.9610 0.7400 1.1272 0.8272 1.3893 1.2049 0.8328 1.4862 0.6192 1.3942 1.0972 1.2201 2.2945 0.6684 1.8830 0.8896 1.6649 1.4518 0.6330 0.7226 1.6361 0.7663 0.4384 0.4814 1.2503 0.6046 0.8903 1.4788 0.7239 0.7616 0.3383 0.3220 0.6864 0.9701 0.4542 1.3152 0.5472 0.9928 0.9084 0.4382 0.6197 0.4820 1.4191 1.2754 1.6586 0.9714 Fumaric acid 1.8194 0.9211 1.0535 0.4130 0.7664 0.6588 0.9730 0.7058 0.8957 1.5145 1.2229 1.1352 3.4559 0.8227 0.6764 1.0230 1.8227 2.2622 0.6495 0.7443 0.4767 0.8038 0.2447 0.2483 1.3466 0.4216 0.5986 0.1115 0.9847 2.3376 1.1399 0.7843 0.2701 0.8739 1.0238 0.3896 0.5402 0.6897 0.4114 1.1209 0.3357 0.4905 0.9807 0.7328 1.0467 1.6566 0.5694 0.4642 0.6712 4.2482 2.2423 0.6440 0.5636 Galactinol 4.7718 0.6282 0.6149 0.1973 1.0889 7.6186 4.4077 0.9475 3.2182 0.4577 0.6462 0.9238 0.5954 1.3176 0.9128 0.9354 1.0331 1.6857 4.7418 1.3305 0.9430 0.4429 0.0745 0.7846 0.1622 0.2403 0.3135 0.3321 0.4891 0.6227 0.3915 0.1671 0.3850 0.2326 0.3106 1.6799 0.4894 0.4798 0.4899 0.1042 0.3803 0.7989 0.1747 0.1503 0.2687 0.2112 1.1783 0.7396 0.8823 0.5688 0.1698 0.1351 0.1341 Galacturonic acid 0.1822 0.1822 0.1822 0.1822 0.1822 0.1822 0.1822 0.1822 0.1822 0.1822 0.1822 0.1822 0.1822 0.1822 0.1822 0.1822 0.1822 0.1822 0.1822 0.1822 0.1822 0.1822 1.3689 0.1822 1.4083 1.0118 0.1822 0.1822 0.3643 5.8777 0.1822 2.0100 1.4089 2.6217 3.1553 1.4198 0.1822 0.1822 0.1822 0.1822 0.8118 0.1822 0.1822 3.2658 0.1822 5.2975 0.1822 1.5508 0.1822 7.6533 7.0343 0.1822 0.1822 Gluconic acid 0.2074 0.2074 0.2074 0.2074 0.2074 0.2074 0.2074 0.2074 0.2074 0.2074 0.2074 0.2074 0.2074 0.2074 0.2074 0.2074 0.2074 0.2074 0.2074 0.2074 0.2074 0.2074 0.2074 0.7253 2.0219 1.0046 0.2074 0.2074 2.6183 2.9679 0.2074 2.0601 2.8911 0.2074 4.5545 0.4147 0.2074 0.2074 0.2074 0.2074 0.2074 0.2074 0.2074 2.5417 0.2074 3.2800 0.2074 0.2074 0.2074 6.2837 5.9095 5.8453 2.0018 Glucose_A 0.6783 0.8600 0.7466 1.0853 0.8823 1.4927 0.8154 1.1792 1.2015 1.6909 3.1795 0.6720 0.9138 0.8666 0.9038 0.8648 0.6865 1.1668 1.2377 0.7670 1.8852 0.9019 0.9764 1.2524 0.8721 0.7542 0.6612 0.8320 0.7556 0.6061 0.8506 1.0062 0.9473 0.9088 1.1321 0.9188 0.8212 0.5340 1.1253 1.0100 1.0681 0.8127 1.4027 1.1684 0.8521 0.9544 0.7814 0.5070 0.7969 0.9977 0.9818 0.8214 1.2129 Glucose_beta 0.8207 1.2694 0.5227 1.2942 0.6116 1.4715 0.9641 1.3246 0.0298 1.6184 3.1169 0.4063 2.0464 0.7224 0.6391 1.0585 0.4518 1.3952 0.7853 1.2783 2.3680 1.2346 0.5513 0.9632 0.5867 0.9303 0.3791 0.6101 0.6868 0.4121 1.0196 1.3014 0.8948 0.9373 0.7070 1.0214 0.4236 0.3959 1.5028 2.3731 0.9085 0.4938 0.8971 0.9864 1.4019 1.3733 0.6349 0.7655 0.8143 0.5871 0.9880 0.4997 1.5234 Glutamic acid 0.0604 0.3873 1.0680 0.3343 1.1079 0.5296 0.2759 0.4068 1.1353 0.7805 0.3706 0.3523 0.4030 0.9002 0.4455 0.6770 0.0604 1.6416 0.7340 0.6857 0.7129 0.6427 0.1219 0.1502 0.4934 0.1949 0.4779 0.2190 0.6417 2.4437 0.7621 2.5022 2.4258 1.7543 1.9437 1.9821 1.5142 0.5522 2.2325 1.0004 0.9763 0.4441 1.2905 1.4190 1.4579 0.9166 1.1607 2.0240 0.9321 0.7399 2.6244 2.1316 1.7588 Glutamic acid (dehydrated) 1.1062 0.9990 0.7891 0.7707 1.4059 1.3728 0.9478 1.0249 0.7870 0.9518 0.7909 0.9689 1.0837 0.8992 1.5492 1.1983 1.0169 0.9246 0.6315 1.3808 1.1520 1.3143 0.7844 1.0548 0.9340 1.0017 1.1281 1.4282 0.8290 0.8501 0.7375 1.0421 0.8975 0.6032 0.9342 0.9887 1.0832 1.0790 1.0066 0.7990 1.0086 0.7830 0.7769 1.1005 0.9775 0.8625 0.7721 0.9427 0.7275 0.9231 1.4351 1.2293 1.2142 Glutamine 1.8064 0.5976 0.3301 1.9407 1.4915 1.6602 0.5209 0.8310 1.6888 1.4445 2.5087 0.4548 0.5064 0.6945 1.5446 1.6572 1.5206 2.0249 2.0142 0.2912 1.2320 1.2276 1.5739 1.1023 0.5541 0.4534 0.4485 1.0469 0.4123 0.5601 0.5791 0.5437 0.5719 0.5127 1.4570 0.6358 1.3327 0.6544 0.7510 0.2754 1.5584 0.2627 2.2697 0.3105 0.2790 0.6003 1.2220 1.5487 0.4078 1.7103 0.3527 0.4685 0.5560 Glyceric acid 1.2961 1.4651 0.5065 0.7711 0.8790 1.4459 1.4577 2.0993 1.4141 0.6606 0.5204 0.7251 1.1093 0.2861 0.7402 1.6401 1.1967 0.5074 0.3028 1.3210 0.3539 0.4834 0.7877 0.5987 0.7517 0.3511 0.3481 0.8322 0.3938 4.7094 1.2660 1.0805 1.1667 0.7884 1.3148 1.4276 1.8478 1.5023 1.2584 0.7487 1.9440 0.1154 0.5960 1.7006 0.4818 1.1315 0.4493 1.3452 0.5653 1.0108 1.2264 0.0260 0.0521 Glycerol 0.0829 0.5110 0.9002 0.3566 0.7526 1.7972 0.4810 0.8104 0.2205 0.6960 0.1517 0.4471 0.3370 0.3488 1.3084 0.8325 0.8139 0.7034 0.4904 2.8588 1.0105 1.5446 0.4222 4.2261 1.1399 2.9978 0.6975 3.2077 1.1111 0.9171 0.3751 0.7492 0.6637 1.3241 1.4876 0.6455 0.8362 1.0289 0.5550 0.7005 0.8857 0.3707 0.5973 0.5087 0.4535 0.9639 1.0555 3.7707 1.2500 0.8514 0.4892 0.5420 0.7207 Glycerol -1-P 0.9789 0.9974 1.9398 1.3095 1.5533 1.1439 0.8742 0.8308 1.1992 2.2529 1.6109 2.0723 0.7378 1.2187 1.5590 0.8958 1.3960 0.1583 1.3220 1.4249 0.8920 0.8148 0.4807 3.5663 0.4634 0.4368 0.7637 0.8106 0.4051 0.6271 1.2508 0.6731 0.5268 0.8155 0.6088 0.7615 0.4613 0.5524 1.4791 0.8036 0.4699 0.9852 1.1401 0.6045 1.4520 0.8923 1.1260 0.5740 0.7576 0.7528 0.4342 0.1583 0.9841 Glycine 1.2364 1.0904 0.8847 0.6583 1.0232 1.7907 0.9131 0.8540 0.5285 0.8454 0.9594 1.0553 0.9053 0.7804 1.3292 0.9369 0.8632 2.9040 0.8101 0.6987 0.9991 3.0538 0.5722 1.6431 1.2355 1.3117 0.9061 0.9817 1.4790 1.0741 0.7887 0.6265 0.5515 0.6101 1.3239 0.8794 0.9031 0.4684 0.5579 1.0091 0.8882 0.6154 0.8637 1.1895 0.9083 0.6564 0.6137 1.2384 0.6314 0.6529 1.2432 0.6995 0.7575 Glycolic acid 0.9225 1.2295 1.2257 0.9129 1.1765 1.3054 1.6320 1.2351 1.5038 0.9565 1.0446 0.7927 1.1639 1.2331 0.9175 1.7932 0.2673 1.5853 0.8413 1.0657 1.2133 1.0279 0.6359 0.6862 0.8986 1.0067 0.6137 1.1401 0.6457 1.0558 0.8604 0.8071 0.6562 0.5484 0.6698 1.4146 0.7235 0.9633 0.8048 1.5366 0.9113 0.3319 0.7826 1.3501 0.8444 0.7135 0.7477 0.8171 0.6800 0.8768 1.2113 1.3610 1.6593 Heptadecanoic acid 0.3544 0.3544 0.3544 0.3544 0.3544 0.3544 0.3544 0.3544 0.3544 0.3544 0.3544 0.3544 0.3544 0.3544 0.3544 0.3544 0.3544 0.3544 0.3544 0.3544 0.3544 0.3544 2.2444 8.6085 1.5450 2.6620 0.3544 0.3544 1.1709 2.6842 0.3544 2.3608 1.0103 2.9535 1.6416 0.3544 0.3544 0.3544 0.3544 0.3544 2.5454 0.3544 0.3544 1.7071 0.3544 2.1599 0.3544 0.7557 0.3544 2.4146 1.5227 1.9005 0.7088 Iminodiacetic acid 0.8520 0.9034 1.0655 0.8128 1.1046 0.7708 0.9930 0.9210 1.0234 1.4681 0.9675 1.0439 1.1448 0.9168 1.4953 0.9875 0.8830 1.3813 0.8048 1.1242 0.8073 1.1921 0.7593 1.0403 0.9473 0.7700 0.8470 0.8701 1.0762 1.1984 0.8392 0.9559 1.0971 1.0385 0.9841 1.0819 1.3829 0.4201 0.9254 1.1869 1.1778 0.6895 1.0884 0.7469 1.0776 0.7929 0.7074 1.4133 0.9074 0.9858 1.1300 1.1762 1.0232 Isoleucine 1.3509 0.8433 0.6355 1.4711 1.1730 1.2929 0.7840 1.0137 1.4228 1.3101 1.9738 0.8946 0.8325 1.0819 1.2216 1.2043 1.1232 1.4123 1.5877 0.5870 1.1203 0.8854 1.1315 1.4554 0.7708 0.9524 0.5733 0.6925 0.5676 0.5747 0.7676 0.7635 0.6969 0.8740 1.0219 1.0532 0.9832 0.7614 0.8427 0.9751 0.9082 0.7463 1.6424 0.7288 0.8618 1.0691 1.0157 1.0772 0.9247 1.2920 0.8541 0.5772 0.6231 Isopropyl beta--thiogalactopyranoside 0.4321 0.8850 0.7766 0.6742 0.7383 1.2192 0.3857 0.7973 0.8222 2.8080 4.3321 0.6928 0.9988 0.6251 0.7454 0.7374 0.9589 1.1158 0.7825 0.5984 5.1951 0.6336 0.5201 0.7042 0.7597 0.5346 0.4534 1.0107 0.6119 0.6674 0.7322 0.9256 0.8189 0.9209 1.7724 0.7716 0.7395 0.8567 0.6127 0.7738 0.7338 0.7997 1.1457 1.8461 1.2994 0.8298 0.5247 0.8700 0.6920 0.8403 0.5818 0.8379 0.8581 Lactate 1.0074 0.4764 0.7291 1.4187 1.2448 1.2171 0.4920 0.9521 1.8328 1.4511 1.8947 0.8567 0.4414 1.6531 0.7993 0.9028 0.9393 0.8892 1.5199 0.5091 3.0532 0.6846 1.0457 1.4730 0.6564 0.6870 1.1599 0.5661 0.4353 0.4630 1.2025 0.4854 0.5955 0.8519 1.1802 1.4887 0.7522 0.3288 0.7089 0.7026 1.0438 0.5708 1.9330 1.0049 0.8347 0.6596 1.8343 0.9386 0.6921 1.0374 0.4573 0.9375 1.3081 Leucine 1.3266 0.7744 0.6335 1.4704 1.1698 1.2637 0.7385 0.9640 1.5460 1.3381 2.0855 0.8748 0.7493 1.1055 1.1858 1.2196 1.1158 1.4243 1.5884 0.5388 1.1875 0.8730 1.1867 1.4197 0.7630 0.9372 0.6045 0.6690 0.5492 0.5218 0.7654 0.7419 0.6476 0.8946 1.0373 1.1369 1.0067 0.7546 0.8261 0.9709 0.8826 0.7287 1.7066 0.7490 0.8557 1.0558 1.0596 1.0766 0.9497 1.2967 0.8343 0.5605 0.6376 Linoleic acid 0.2632 0.7446 0.4720 0.2988 0.4554 1.6923 0.3720 0.9752 0.5430 1.1139 0.4700 0.3943 0.2237 0.5444 0.6461 0.7663 0.9114 0.5258 0.4752 4.5367 0.9230 0.6078 0.6105 4.7184 0.8310 2.2739 0.7395 2.4414 0.8357 0.9538 0.5562 1.2738 0.7228 1.0350 2.3629 0.7732 0.6483 1.2430 0.8321 0.7143 1.0289 0.4192 0.8447 0.8125 0.6208 1.0881 1.2392 1.3143 0.8387 1.0201 0.5570 0.9155 0.7799 Lysine 2.3045 1.6761 1.2135 1.4088 1.0513 0.5552 0.4950 0.1324 2.3720 1.4294 0.1324 1.5937 0.6701 1.5445 0.1324 0.9863 0.1324 1.3689 1.3293 0.8901 0.9295 1.3257 0.8434 0.4465 1.1635 0.9059 0.9311 0.9406 0.9333 0.6355 1.3939 0.9665 0.1324 0.9542 0.7882 1.0749 1.4495 0.2676 1.4381 1.6534 0.1324 0.3551 1.3236 0.9961 1.3706 0.4794 1.0795 0.8896 0.7235 0.7348 0.1324 2.2422 1.9482 Malic acid 0.3388 0.3388 0.3388 0.3388 0.3388 0.3388 0.3388 0.3388 0.3388 0.3388 0.3388 0.3388 0.3388 0.3388 0.3388 0.3388 0.3388 0.3388 0.3388 0.3388 0.3388 0.3388 0.3388 0.3388 3.1729 2.5667 0.3388 0.3388 0.3388 5.5066 0.3388 2.3531 0.3388 3.8166 1.9801 0.3388 0.3388 0.3388 0.3388 0.3388 3.9197 0.3388 0.3388 3.1872 0.3388 4.6749 0.3388 0.3388 0.3388 0.3388 4.6014 1.7226 1.6070 Malonate 1.1706 0.5331 0.5625 1.1483 0.8967 4.2198 0.6566 4.0668 1.1096 0.8968 1.8808 0.5169 0.5150 0.6831 0.7373 0.9363 0.9359 1.2323 1.1890 0.3690 0.5850 0.6027 0.9042 0.8837 0.8033 0.5474 0.5029 0.7294 0.5749 0.7383 0.6881 0.7106 0.7235 0.5842 1.8243 0.7108 0.8195 0.8779 3.4985 0.5466 1.1591 0.5340 1.4192 1.3297 0.5976 0.6378 0.6596 0.7697 0.6739 1.2162 0.7142 0.5041 0.6725 Mannitol 3.6008 1.4870 0.2702 0.2709 0.6005 2.6506 0.5026 1.1734 0.1581 0.2224 0.3411 0.3430 2.1418 0.0134 6.1296 0.2331 0.0134 0.2052 0.5619 0.0749 1.2174 0.1034 0.0134 0.0134 5.8777 1.1710 1.1416 1.6241 0.0134 3.0857 2.3968 0.4678 5.0486 0.3406 0.2753 0.3435 0.7264 0.2476 0.4124 0.3008 0.0134 0.1187 3.4310 1.3392 0.1488 1.4908 0.0134 0.1265 0.0573 0.0134 0.0134 0.2093 0.2102 Melezitose 0.0961 0.0961 0.0961 0.0961 0.0961 0.0961 0.0961 0.0961 0.0961 0.0961 0.0961 0.0961 0.0961 0.0961 0.0961 0.0961 0.0961 0.0961 0.0961 0.0961 0.0961 0.0961 0.0961 2.4313 2.4591 4.1960 0.0961 0.0961 5.0483 6.5515 0.0961 3.4420 1.7007 0.8345 1.4763 0.7279 0.0961 0.0961 0.0961 0.0961 0.0961 0.0961 0.0961 2.9438 0.0961 3.2714 0.0961 0.0961 0.0961 9.8561 3.9722 0.4367 0.0961 Methylamine 3.0544 0.0069 0.0069 3.3130 2.2924 1.6633 0.0069 0.0069 1.7794 1.7204 3.1918 0.0069 0.2226 0.0069 2.2286 2.6362 1.9074 3.0564 2.6478 0.0069 1.8657 1.8501 2.2867 0.0069 0.0069 0.0069 0.0069 0.0138 0.0069 0.0405 0.0069 0.0069 0.0069 0.0069 1.7534 0.0069 1.4973 0.0069 0.0069 0.9302 0.8052 0.0069 3.6304 0.0069 0.0069 1.5173 1.7596 2.1591 0.0069 2.7274 0.2769 0.0069 0.0069 Methylguanidine 1.8942 0.0771 0.0025 2.7256 1.6468 1.2485 0.2145 0.0025 1.4020 1.2704 2.3488 0.0025 0.0025 0.6436 1.1872 2.1565 1.3596 2.1716 1.8745 0.0025 1.2225 1.7522 2.4486 3.1217 0.6508 0.0025 0.3899 2.4122 0.1707 0.0025 1.3370 0.0025 0.1805 0.0025 2.1416 0.6743 1.0617 0.2168 0.5543 0.0025 4.0713 0.0025 2.9373 0.0025 0.0025 0.0025 1.4619 2.0671 0.0025 1.8626 0.0025 0.0025 0.0025 Myristic acid 0.0722 0.5704 0.5005 0.1847 0.5325 0.5764 0.3467 0.6971 0.5284 0.7893 0.3015 0.2588 0.1444 0.4402 0.6574 0.8935 0.6751 0.6735 0.3019 2.8697 0.8149 0.8456 0.4622 2.0349 1.4060 2.0505 0.9187 3.7525 1.4051 2.0408 0.6974 0.8574 0.6470 1.9408 1.1856 1.0767 1.6342 1.5047 0.9079 0.6277 1.0881 0.3718 1.5008 0.6661 0.6898 1.6735 1.0537 1.7618 0.9114 0.8577 1.1737 1.1314 1.2959 Nicotinic acid 0.0347 1.0648 0.8657 0.9489 0.0347 0.0347 2.0998 0.0347 0.0347 0.0347 0.0347 0.7233 0.7696 1.3987 0.7692 2.4137 0.0347 0.0347 0.0347 1.2195 1.7495 1.3579 1.2615 1.3920 1.2262 0.7280 1.2108 2.8168 1.1855 1.2945 2.0114 0.5226 0.4998 0.7067 0.9799 0.8382 0.0347 1.4710 1.1793 1.5245 2.4334 1.1759 0.6041 1.3590 1.2874 1.7742 1.5072 1.0183 0.0695 1.0852 1.9554 1.0455 1.0732 N-methylhistidine 5.0316 0.7113 0.4574 1.3798 1.1745 1.0144 0.8295 1.5679 4.4031 0.9846 6.4377 0.9428 0.6209 1.0016 0.8897 0.9578 1.3166 2.2412 2.2259 0.3597 0.5745 0.5159 0.7268 0.6027 0.4175 0.4550 0.3406 0.3341 0.3262 0.3287 0.5159 0.4621 0.5464 0.3499 0.3945 0.7193 0.6556 0.9461 0.6528 0.4506 0.5991 0.7229 1.0432 0.4186 0.7139 0.4576 0.6192 0.3118 0.8514 0.8091 0.5080 0.5022 0.5797 Norleucine 0.6615 1.2051 0.9471 1.1129 1.8970 0.3561 0.6144 0.6108 2.1603 0.7802 1.2816 0.8244 0.8639 2.7003 1.2897 1.1818 1.0248 1.0448 0.7842 1.2160 1.1084 0.6571 1.2753 0.3164 0.6861 0.9056 1.1521 0.9796 0.7001 0.5169 0.5571 1.1746 0.7937 1.9622 0.8055 2.1934 1.4361 0.4409 0.7848 1.1522 0.5735 0.5502 1.2759 0.7936 0.8133 0.5473 0.9103 0.5073 0.7307 0.9041 1.3924 0.8014 1.0447 Norvaline 0.1471 0.5503 0.6738 1.0526 0.8699 0.8183 0.5485 0.4898 2.4702 1.5586 1.0515 1.0175 0.7122 1.4920 0.6041 1.0257 0.7549 1.1494 1.1738 1.2446 1.8736 0.7806 1.0378 0.3641 0.8613 1.1069 1.1296 1.2573 0.9751 0.4142 0.7005 1.0975 0.7457 1.2235 0.9773 1.9012 1.0881 0.8884 0.8568 1.4103 0.4742 0.4607 0.8413 1.0208 1.0397 0.7299 1.2556 0.6503 1.1619 1.2325 1.0077 1.4708 1.5602 Oleic acid 0.1300 0.6703 0.6943 0.1298 0.5876 0.6233 0.2900 1.1189 0.4817 0.7212 0.2032 0.2326 0.1069 0.3612 0.6713 0.6861 1.2978 0.3955 0.2443 3.0335 1.2153 0.4504 0.7077 2.0094 0.7720 2.2598 0.5058 2.7099 1.3073 1.6373 0.9486 1.4167 1.0180 2.2261 1.4700 0.7204 1.2367 1.9805 1.2415 0.6168 1.2299 0.3190 1.0963 0.8580 0.6292 1.1740 1.3564 1.8241 0.8059 1.5820 0.7390 1.1533 1.1030 Ornithine 0.1127 1.8392 1.0850 1.0378 1.0138 1.2426 1.0620 0.6532 1.8654 1.1344 0.9741 0.9933 1.0181 1.3270 0.7943 1.0350 0.9780 1.2495 1.3548 1.2802 0.9364 1.4799 0.7557 0.5156 1.4692 0.4999 0.7551 1.0769 0.7189 1.1140 0.6158 1.3973 0.8676 0.9259 0.8723 1.0716 1.6999 0.6032 0.8954 1.1468 1.3841 0.1127 1.2877 0.8027 0.9846 0.6623 0.7551 0.7721 0.7211 1.0390 0.9855 1.1046 0.9206 Oxalic acid 0.0311 1.7969 1.3284 1.5849 1.7358 2.8092 2.4970 3.1851 2.1196 0.0311 0.0311 1.5145 1.3704 1.9163 2.4953 3.7326 0.0311 0.0311 0.0311 0.0311 0.0311 0.5714 0.5248 1.0121 0.5566 0.5814 1.1227 0.7251 0.8407 0.8550 1.4722 0.0311 0.5589 0.5433 0.8708 0.0311 0.5420 0.8736 0.3473 0.7400 0.8283 1.3101 0.0311 0.6224 1.0163 0.9487 1.5634 0.6249 1.4718 0.4781 1.2140 0.8832 0.8728 Palmitic acid 0.4788 0.8419 0.7151 0.4465 0.8874 0.8375 0.4684 1.0399 0.7325 1.1909 0.7663 0.5673 0.3989 0.6047 0.7911 0.7836 1.0287 0.7230 0.5620 2.3430 1.3135 0.7338 0.7727 2.2193 0.8373 1.5135 0.7346 1.7750 1.0619 1.2337 0.7515 1.0429 0.9688 1.8516 1.1500 0.9991 0.9475 1.3569 1.1901 0.8153 1.1188 0.5096 1.2732 1.0018 0.7572 1.1419 1.3421 1.4706 0.8358 1.1311 0.8238 1.0685 1.0489 Palmitoleic acid 0.0421 0.5876 0.0421 0.0421 0.7747 0.2868 0.0421 0.8672 0.3727 0.6672 0.0421 0.0421 0.0421 0.0421 1.0317 0.5025 1.1764 0.0421 0.0421 3.4840 1.2897 0.4417 0.4088 1.4308 0.9428 4.5583 0.3203 4.0541 1.1790 2.3729 1.3715 0.9616 1.3353 3.0869 1.4428 0.9139 0.7462 0.9074 1.5325 0.4771 0.9207 0.0421 1.1082 0.2431 0.5473 0.8527 1.7381 3.4947 0.5873 0.9804 0.5169 1.1101 0.9133 Phenylalanine 10.4594 0.6726 0.6341 1.0203 1.2218 1.0294 0.9702 1.5450 4.0419 2.1732 5.3745 0.5118 0.9223 0.6237 1.1606 0.8633 0.0234 0.0234 0.8305 0.8595 0.3001 0.5261 0.7890 0.7894 0.4543 0.3209 0.2140 1.1346 0.5317 0.7006 0.6146 0.7602 0.9470 0.4358 0.4035 0.7795 0.2907 0.5096 0.4791 0.8043 0.9464 0.5445 1.0208 0.0234 0.3282 0.4230 0.5173 0.1799 0.3421 0.7037 0.6955 0.4826 0.0468 Phosphoric acid 1.1951 1.0072 1.0999 0.7311 1.1966 1.0189 0.9673 0.9306 1.0602 1.2002 0.9643 1.3208 0.9945 1.2028 1.4460 0.9342 1.2572 0.8104 1.0597 1.3406 0.7602 0.9885 0.6828 1.3166 1.6014 0.7427 0.5654 1.0170 0.7971 0.9149 0.8945 0.8415 0.7197 0.8510 0.7979 1.1283 0.8659 0.8878 0.7260 1.3389 0.7450 1.1641 0.9892 0.8190 1.0874 0.7412 0.9542 1.0895 1.0996 1.0899 1.1406 0.9190 0.9856 Porphine 1.5714 2.0162 0.7168 0.9750 0.8453 1.2460 1.7118 1.7521 1.2708 1.1572 0.9543 0.8533 0.7508 1.1589 1.4343 1.9782 0.1041 2.7760 1.0487 0.6381 0.1041 0.8969 0.4605 1.1670 0.8962 0.6851 0.4652 0.8526 1.1692 0.6531 1.1925 0.3588 0.7644 0.4962 0.5889 0.5790 0.4896 0.8393 0.6853 0.6654 1.1377 0.8541 0.6503 1.6868 0.9157 0.6479 1.1752 1.0223 1.4445 0.7280 1.5471 1.0729 1.1487 Proline 1.7052 1.1401 1.2658 1.1666 1.0417 1.1176 0.8771 0.6694 0.8996 1.0180 2.1662 1.4888 1.6783 1.7622 1.4861 1.2955 0.7723 1.5739 0.8449 0.6400 1.2665 1.1809 0.4076 0.4388 0.8755 0.3059 1.0832 0.7991 0.5661 0.7313 0.4676 1.1603 0.8544 0.4284 0.9942 1.7327 1.2870 0.5592 0.7061 1.1166 1.1786 0.2553 1.2539 0.7278 0.8937 0.5998 0.7619 0.8409 0.6187 1.1238 1.8068 0.5607 0.8077 Pyrophosphate 0.0917 0.0917 0.0917 0.0917 0.0917 12.4790 3.0135 0.0917 0.0917 0.0917 0.0917 0.0917 0.0917 0.0917 0.0917 15.6833 0.0917 0.0917 0.0917 0.8598 0.0917 0.2866 0.0917 0.1933 0.3711 0.0917 0.7082 0.0917 0.3328 0.0917 6.0499 0.0917 0.0917 0.0917 0.2657 0.0917 0.0917 0.0917 0.0917 0.7219 0.5639 0.0917 0.0917 0.0917 1.3020 0.9039 0.6705 0.4544 0.0917 2.6528 0.6939 1.2832 0.5770 Pyruvic acid 0.0315 0.8750 1.9363 0.9342 0.2661 1.4261 0.0631 1.2121 0.8264 2.8087 1.3202 0.4432 1.1513 0.7386 0.0315 0.6579 0.7702 1.5698 0.8178 0.9975 3.8360 2.0927 0.1766 0.6391 1.0550 0.4024 1.1375 0.6404 0.5084 0.5131 1.6391 0.2867 0.9391 0.9998 0.6283 2.5765 0.2268 1.1006 1.6341 1.3174 0.3650 0.9833 1.4024 1.2393 1.6028 0.8872 0.6394 0.3006 0.9597 0.8141 0.4825 0.9755 1.1215 Serine 0.7439 1.6189 0.8057 0.2549 1.4400 0.8223 0.4205 0.9651 1.1748 1.1015 0.7805 0.7219 0.7059 1.0700 0.6214 0.5659 0.9661 1.1193 0.5664 1.3847 1.6310 1.6927 0.6848 2.2979 1.7549 1.5201 0.7885 1.1452 1.2743 0.8943 0.5327 1.5748 0.4471 0.9537 1.1312 2.0263 1.0741 0.7725 1.1798 1.0770 1.3107 0.3351 0.7613 1.0497 1.0052 0.9003 0.5357 0.8399 0.6682 0.9971 0.8583 0.7785 0.6578 Serotonin 0.1694 0.1694 1.6513 0.1694 1.6367 0.1694 1.1616 0.1694 0.1694 0.1694 0.1694 0.1694 0.1694 1.5927 1.6796 1.3377 0.1694 0.1694 0.1694 1.8505 1.9686 1.6461 2.7705 1.6213 1.3249 1.6115 1.1537 1.6746 0.5976 1.2767 1.2284 1.1106 0.1694 0.4892 0.6304 1.8531 0.1694 0.1694 1.7669 1.7308 1.6589 0.1694 1.5105 1.2346 1.9316 1.1217 1.6043 0.6976 0.1694 1.1523 1.4397 1.1783 1.0562 Stearic acid 0.7289 0.8848 1.1133 0.5990 0.9369 1.0710 0.7259 1.0069 0.7780 1.0078 0.6542 0.6655 0.4738 0.8295 1.1288 0.8176 0.8293 0.7266 0.6060 1.7834 1.1473 0.8459 0.8334 1.9261 1.0977 1.0108 0.8033 1.1134 0.9273 1.1513 0.7223 1.1346 0.8333 1.3206 1.0277 1.0550 1.1829 1.1614 1.2843 0.8727 1.1674 0.7610 1.2030 1.1063 0.9237 1.1807 1.1922 1.3856 0.8173 1.2793 0.9966 1.0987 1.0699 Succinate 2.3413 0.2837 0.0952 2.1976 1.6243 1.8156 0.1231 0.6435 1.8352 1.5739 2.9412 0.2215 0.4300 0.3483 1.6218 1.9163 1.7469 2.4963 2.2883 0.0132 1.1704 1.2510 3.5640 0.4501 0.3838 0.3885 0.0786 1.3549 0.3075 0.4684 0.1169 0.4234 0.1971 0.4471 1.5370 0.2828 1.5171 0.7161 0.5984 0.0793 1.3000 0.0132 2.4468 0.2626 0.0132 0.7800 1.1712 1.6252 0.1657 1.8463 0.4135 0.6008 0.4714 Sucrose 0.1993 0.4870 19.2371 0.2090 0.5065 0.2890 0.3292 0.1240 0.0645 0.0071 3.0035 0.2211 4.3389 0.0071 0.1601 0.0909 9.0549 0.1787 0.0579 0.0698 0.2355 0.0071 0.0071 0.0769 0.1746 0.0071 0.0071 0.0071 0.0071 1.8656 0.4328 0.0071 0.0071 0.0071 0.0071 0.0071 0.0071 0.1884 0.0231 0.0071 0.2070 0.3244 0.0071 0.0071 0.0071 0.4719 0.0071 0.0071 0.0071 9.3080 0.9070 0.0071 0.0071 Tagatose 0.1397 0.1397 0.1397 0.1397 0.1397 0.1397 0.1397 0.1397 0.1397 0.1397 0.1397 0.1397 0.1397 0.1397 0.1397 0.1397 0.1397 0.1397 0.1397 0.1397 0.1397 0.1397 1.0104 0.1397 0.6943 0.7865 0.1397 0.1397 1.2764 2.9226 0.1397 4.2844 1.1636 1.3141 2.7904 0.5520 0.1397 0.1397 0.1397 0.1397 15.6897 0.1397 0.1397 3.8134 0.1397 2.5659 0.1397 0.4024 0.1397 0.6118 1.4216 2.7553 4.0571 Threitol 2.2222 0.6299 0.6450 0.7333 0.7286 2.6267 0.5314 1.0984 0.6009 0.7895 0.8571 1.2191 3.8148 0.5598 0.4447 0.5366 1.9124 1.5399 0.7525 0.3796 0.5142 0.4873 0.3620 0.2918 1.1101 0.4571 0.2903 0.3519 0.5635 3.3444 0.9383 1.1795 0.7396 0.9215 1.2796 0.6096 0.4304 1.1210 0.4351 0.7614 0.4869 0.4782 0.8454 0.8519 0.8895 2.0749 0.6798 1.6576 0.3348 2.2464 1.7105 1.0130 0.9201 Threonine 0.7987 1.8013 0.7677 0.6044 1.1532 0.6425 0.8470 0.5440 1.4703 1.1277 1.0619 0.6963 0.8925 1.4004 0.6944 0.5635 1.1543 1.3968 1.0384 1.2225 1.6764 1.8549 0.7135 1.9855 2.1520 0.9375 1.0601 1.5600 0.7875 0.7931 0.4823 0.8364 0.5414 0.4912 0.7013 1.7818 1.5153 0.4704 0.7989 1.0966 1.2224 0.2603 0.5778 0.7319 0.7726 0.5528 0.8450 0.6680 0.5290 1.1182 1.3098 1.2477 1.0504 Tryptophan 0.2717 0.2717 0.2717 0.2717 0.2717 0.2717 0.2717 0.2717 0.2717 0.2717 0.2717 0.2717 0.2717 0.2717 0.2717 0.2717 0.2717 0.2717 0.2717 0.2717 0.2717 0.2717 2.0979 1.9259 1.5029 1.0160 0.2717 0.2717 0.7186 2.2298 0.2717 2.5587 0.9169 2.4935 1.3322 4.4253 0.2717 0.2717 0.2717 0.2717 2.0685 0.2717 0.2717 2.0398 0.2717 2.1039 0.2717 3.7600 0.2717 2.6023 3.8108 4.0148 2.1460 Tyrosine 5.0127 0.6084 0.6821 1.0069 1.4127 0.9602 0.6932 1.0984 4.7475 0.5722 6.7879 1.0030 0.6277 0.8360 1.0584 0.9233 1.9353 3.1174 3.0664 0.4133 0.6064 0.4351 0.6015 0.5800 0.5224 0.5246 0.4250 0.5592 0.2230 0.2543 0.3900 0.3058 0.4660 0.6813 0.5304 0.5483 0.4772 0.8135 0.6304 0.5952 0.5095 0.6190 0.6963 0.5167 0.4541 0.4140 0.3854 0.4661 0.5896 0.5268 0.3577 0.2992 0.4328 Urea 1.9774 1.0258 0.9889 0.8517 0.6518 0.6208 0.6757 0.7367 0.8613 1.2831 1.0304 1.3880 2.0673 0.7764 0.6155 0.8978 2.1977 1.8771 0.6110 0.8537 1.0878 0.8365 0.4310 0.3319 1.0890 0.5436 0.5969 0.5542 0.8693 2.1500 1.1606 1.4502 0.8902 0.8673 0.9551 0.7386 0.8418 1.1235 0.6795 1.0728 0.5156 0.6944 0.9999 0.8430 1.1114 1.2370 0.6501 0.5162 0.6363 2.1158 1.8034 0.7410 0.8781 Uric acid 2.0682 0.4734 1.2609 0.4569 0.9362 1.7984 0.4135 1.1774 0.6347 0.7460 0.9328 0.7744 3.8107 0.8085 0.5898 0.6182 1.8388 1.1334 0.5375 0.2325 0.7569 0.2048 0.5669 0.1895 0.4972 0.3636 0.7367 0.5546 0.5761 2.6392 1.1114 1.4228 0.7571 1.1181 0.7669 1.2082 0.5837 1.2852 0.5981 1.0207 0.3658 0.3409 1.2338 0.6865 0.9729 1.7658 1.1731 0.5094 0.6486 2.4157 2.4245 1.2379 1.0252 Valine 1.3366 0.7202 0.6521 1.5480 1.2031 1.3070 0.6853 0.8984 1.5641 1.3343 2.1697 0.8955 0.7362 1.0849 1.2220 1.2661 1.1722 1.5195 1.6631 0.4758 1.2142 0.9368 1.2540 1.4669 0.7902 0.9381 0.5279 0.6339 0.4891 0.4486 0.6745 0.6885 0.5555 0.9025 1.0694 1.0680 1.0821 0.7266 0.7953 1.0032 0.8315 0.7192 1.7458 0.7279 0.8363 1.0989 1.0672 1.1569 0.9415 1.3710 0.7892 0.4319 0.5630 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name pubchem_id inchi_key kegg_id other_id other_id_type ri ri_type moverz_quant 2-HB (2-hydroxybutyric acid) MAYO_ID 2-hydroxypyridine 8871 C02502 MAYO_ID 3-AIB (a-amino[1-(14)C]isobutyric acid) MAYO_ID 3-HB (3-hydroxybutyric acid) MAYO_ID Acetate 176 C00033 MAYO_ID Acetoacetate 96 C00164 MAYO_ID Acetone 180 C00207 MAYO_ID Acetylsalicylic acid MAYO_ID Alanine 5950 C00041 MAYO_ID Allo-inositol MAYO_ID Arabitol 827 C01904 MAYO_ID Benzoic acid 243 C00180 MAYO_ID Beta- alanine 239 C00099 MAYO_ID Cholesterol 5997 C00187 MAYO_ID Citrate 311 C00158 MAYO_ID Creatine 586 C00300 MAYO_ID Creatinine 588 C00791 MAYO_ID Creatinine g MAYO_ID Cysteine 594 C00097 MAYO_ID Fumaric acid 444972 C00122 MAYO_ID Galactinol 11727586 C01235 MAYO_ID Galacturonic acid 84740 C08348 MAYO_ID Gluconic acid 10690 C00257 MAYO_ID Glucose_A 5793 C00031 MAYO_ID Glucose_beta 5793 C00031 MAYO_ID Glutamic acid 33032 MAYO_ID Glutamic acid (dehydrated) MAYO_ID Glutamine 5961 C00064 MAYO_ID Glyceric acid 439194 C00258 MAYO_ID Glycerol 753 C00116 MAYO_ID Glycerol -1-P MAYO_ID Glycine 750 C00037 MAYO_ID Glycolic acid 757 C00160 MAYO_ID Heptadecanoic acid 10465 MAYO_ID Iminodiacetic acid MAYO_ID Isoleucine 6306 C00407 MAYO_ID Isopropyl beta--thiogalactopyranoside MAYO_ID Lactate 107689 C00186 MAYO_ID Leucine 6106 C00123 MAYO_ID Linoleic acid 5280450 C01595 MAYO_ID Lysine 5962 C00047 MAYO_ID Malic acid 222656 C00149 MAYO_ID Malonate 867 C00383 MAYO_ID Mannitol 6251 C00392 MAYO_ID Melezitose 92817 C08243 MAYO_ID Methylamine MAYO_ID Methylguanidine 10111 C02294 MAYO_ID Myristic acid 11005 C06424 MAYO_ID Nicotinic acid 938 C00253 MAYO_ID N-methylhistidine C03298 MAYO_ID Norleucine 21236 C01933 MAYO_ID Norvaline 439575 C01799 MAYO_ID Oleic acid 445639 C00712 MAYO_ID Ornithine 6262 C00077 MAYO_ID Oxalic acid 971 C00209 MAYO_ID Palmitic acid 985 C00249 MAYO_ID Palmitoleic acid 445638 C08362 MAYO_ID Phenylalanine 6140 C00079 MAYO_ID Phosphoric acid 1004 C00009 MAYO_ID Porphine 5481287 MAYO_ID Proline 145742 C00148 MAYO_ID Pyrophosphate 644102 C00013 MAYO_ID Pyruvic acid 1060 C00022 MAYO_ID Serine 5951 C00065 MAYO_ID Serotonin 5202 C00780 MAYO_ID Stearic acid 5281 C01530 MAYO_ID Succinate 1110 C00042 MAYO_ID Sucrose 5988 C00089 MAYO_ID Tagatose 1101 MAYO_ID Threitol 169019 C16884 MAYO_ID Threonine 6288 C00188 MAYO_ID Tryptophan 6305 C00078 MAYO_ID Tyrosine 6057 C00082 MAYO_ID Urea 1176 C00086 MAYO_ID Uric acid 1175 C00366 MAYO_ID Valine 6287 C00183 MAYO_ID METABOLITES_END #END