#METABOLOMICS WORKBENCH araskind_20150415_9719251_mwtab.txt DATATRACK_ID:238 STUDY_ID:ST000169 ANALYSIS_ID:AN000263 PROJECT_ID:PR000147 VERSION 1 CREATED_ON 2016-09-17 #PROJECT PR:PROJECT_TITLE Early in life exposures studies PR:PROJECT_SUMMARY Early in life exposures affect innate immune cells activation PR:INSTITUTE University of Michigan PR:DEPARTMENT Pediatrics PR:LABORATORY Hershenson Lab PR:LAST_NAME Popova PR:FIRST_NAME Antonia PR:ADDRESS Ann Arbor, MI PR:EMAIL antoniap@med.umich.edu PR:PHONE 734-764-6736 #STUDY ST:STUDY_TITLE Baby Mice Hyperoxia treatment (G64-N.H-7d14d) ST:STUDY_TYPE In this experiment 2 day old baby mice were exposed to hyperoxia (75% O2) ST:STUDY_TYPE for 14 days. Control baby mice were housed in room air (normoxia). Plasma and ST:STUDY_TYPE lavage fluid (BALF) were harvested after 14 days of exposure (on Day of life ST:STUDY_SUMMARY In this experiment 2 day old baby mice were exposed to hyperoxia (75% O2) ST:STUDY_SUMMARY for 14 days. Control baby mice were housed in room air (normoxia). Plasma and ST:STUDY_SUMMARY lavage fluid (BALF) were harvested after 14 days of exposure (on Day of life ST:INSTITUTE University of Michigan ST:DEPARTMENT Biomedical Research Core Facilities ST:LABORATORY Metabolomics core ST:LAST_NAME Kachman ST:FIRST_NAME Maureen ST:ADDRESS 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 ST:EMAIL mkachman@umich.edu ST:PHONE - ST:NUM_GROUPS 1 ST:TOTAL_SUBJECTS 16 #SUBJECT SU:SUBJECT_TYPE - SU:SUBJECT_SPECIES Mus musculus SU:TAXONOMY_ID 10090 #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS SU0005232 S00016724 Type:BALF | Condition:Hyperoxia SUBJECT_SAMPLE_FACTORS SU0005233 S00016725 Type:BALF | Condition:Hyperoxia SUBJECT_SAMPLE_FACTORS SU0005234 S00016726 Type:BALF | Condition:Hyperoxia SUBJECT_SAMPLE_FACTORS SU0005235 S00016727 Type:BALF | Condition:Hyperoxia SUBJECT_SAMPLE_FACTORS SU0005228 S00016720 Type:BALF | Condition:Normoxia SUBJECT_SAMPLE_FACTORS SU0005229 S00016721 Type:BALF | Condition:Normoxia SUBJECT_SAMPLE_FACTORS SU0005230 S00016722 Type:BALF | Condition:Normoxia SUBJECT_SAMPLE_FACTORS SU0005231 S00016723 Type:BALF | Condition:Normoxia SUBJECT_SAMPLE_FACTORS SU0005232 S00016716 Type:Plasma | Condition:Hyperoxia SUBJECT_SAMPLE_FACTORS SU0005233 S00016717 Type:Plasma | Condition:Hyperoxia SUBJECT_SAMPLE_FACTORS SU0005234 S00016718 Type:Plasma | Condition:Hyperoxia SUBJECT_SAMPLE_FACTORS SU0005235 S00016719 Type:Plasma | Condition:Hyperoxia SUBJECT_SAMPLE_FACTORS SU0005228 S00016712 Type:Plasma | Condition:Normoxia SUBJECT_SAMPLE_FACTORS SU0005229 S00016713 Type:Plasma | Condition:Normoxia SUBJECT_SAMPLE_FACTORS SU0005230 S00016714 Type:Plasma | Condition:Normoxia SUBJECT_SAMPLE_FACTORS SU0005231 S00016715 Type:Plasma | Condition:Normoxia #COLLECTION CO:COLLECTION_SUMMARY - CO:SAMPLE_TYPE Blood plasma, Bronchoalveolar Lavage Fluid #TREATMENT TR:TREATMENT_SUMMARY - #SAMPLEPREP SP:SAMPLEPREP_SUMMARY - SP:SAMPLEPREP_PROTOCOL_FILENAME Gly-TCA-nucleotides_analysis_protocol-2015-03-09.docx #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE HILIC CH:INSTRUMENT_NAME Agilent 1260 CH:COLUMN_NAME Phenomenex Luna NH2 (150 x 1mm, 3um) CH:METHODS_ID AQM015 CH:METHODS_FILENAME QQQ-ATP,ADP,AMP,A-Insert.m #ANALYSIS AN:ANALYSIS_TYPE MS AN:LABORATORY_NAME MRC2 (University of Michigan) AN:ACQUISITION_PARAMETERS_FILE QQQ-ATP,ADP,AMP,A-Insert.m AN:PROCESSING_PARAMETERS_FILE EX00371-Nucleotides-Quant.m #MS MS:MS_COMMENTS - MS:INSTRUMENT_NAME Agilent 6520 QTOF MS:INSTRUMENT_TYPE QTOF MS:MS_TYPE ESI MS:ION_MODE NEGATIVE #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS mM MS_METABOLITE_DATA_START Samples S00016724 S00016725 S00016726 S00016727 S00016720 S00016721 S00016722 S00016723 S00016716 S00016717 S00016718 S00016719 S00016712 S00016713 S00016714 S00016715 Factors Type:BALF | Condition:Hyperoxia Type:BALF | Condition:Hyperoxia Type:BALF | Condition:Hyperoxia Type:BALF | Condition:Hyperoxia Type:BALF | Condition:Normoxia Type:BALF | Condition:Normoxia Type:BALF | Condition:Normoxia Type:BALF | Condition:Normoxia Type:Plasma | Condition:Hyperoxia Type:Plasma | Condition:Hyperoxia Type:Plasma | Condition:Hyperoxia Type:Plasma | Condition:Hyperoxia Type:Plasma | Condition:Normoxia Type:Plasma | Condition:Normoxia Type:Plasma | Condition:Normoxia Type:Plasma | Condition:Normoxia Adenosine 0.7900 0.1100 0.2900 0.6200 0.5100 0.6500 1.4200 0.4500 9.8600 8.5600 7.2600 11.8200 11.8600 7.2100 12.3100 7.1700 ADP 0.8100 0.1300 0.3500 0.8800 0.7200 0.8200 0.6100 0.4700 0.6500 6.7000 1.2100 0.6500 0.3900 0.5000 4.0200 0.4300 AMP 1.7800 0.0900 0.3200 1.0400 0.5700 0.7600 1.6900 0.5200 7.1000 21.5000 7.3100 8.3500 2.9500 2.5400 13.0500 0.2900 ATP 0.1900 0.0000 0.2000 0.2900 0.4400 0.4400 0.1900 0.4300 0.5500 1.7700 0.8500 0.5500 0.5700 0.5200 1.2800 0.9100 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name moverz_quant ri ri_type pubchem_id inchi_key kegg_id other_id other_id_type Adenosine 60961 UM_Target_ID ADP 6022 UM_Target_ID AMP 6083 UM_Target_ID ATP 5957 UM_Target_ID METABOLITES_END #END