#METABOLOMICS WORKBENCH araskind_20150608_9482051_mwtab.txt DATATRACK_ID:285 STUDY_ID:ST000188 ANALYSIS_ID:AN000289 PROJECT_ID:PR000159 VERSION 1 CREATED_ON 2016-09-17 #PROJECT PR:PROJECT_TITLE Murine AxP PR:PROJECT_SUMMARY Plasma Nucleotide/adenosine concentrations PR:INSTITUTE University of Michigan PR:DEPARTMENT Internal Medicine-Cardiology PR:LABORATORY Visovatti Lab PR:LAST_NAME Visovatti PR:FIRST_NAME Scott PR:ADDRESS Ann Arbor, MI PR:EMAIL shv@med.umich.edu PR:PHONE 734-657-6917 #STUDY ST:STUDY_TITLE Murine apyrase ST:STUDY_TYPE This experiment was performed in wild type vs. CD39 knockout (KO) mice that had ST:STUDY_TYPE infusions of either saline or apyrase over the course of 2 weeks. Whole blood ST:STUDY_TYPE obtained via intracardiac puncture, and was drawn into a syringe prefilled with ST:STUDY_TYPE anti-enzyme stop solution. ST:STUDY_SUMMARY This experiment was performed in wild type vs. CD39 knockout (KO) mice that had ST:STUDY_SUMMARY infusions of either saline or apyrase over the course of 2 weeks. Whole blood ST:STUDY_SUMMARY obtained via intracardiac puncture, and was drawn into a syringe prefilled with ST:STUDY_SUMMARY anti-enzyme stop solution. ST:INSTITUTE University of Michigan ST:DEPARTMENT Biomedical Research Core Facilities ST:LABORATORY Metabolomics core ST:LAST_NAME Kachman ST:FIRST_NAME Maureen ST:ADDRESS 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 ST:EMAIL mkachman@umich.edu ST:PHONE - ST:NUM_GROUPS 4 ST:TOTAL_SUBJECTS 15 #SUBJECT SU:SUBJECT_TYPE - SU:SUBJECT_SPECIES Mus musculus SU:TAXONOMY_ID 10090 #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS SU0011768 S00017521 Type:KO | Treatment:apyrase SUBJECT_SAMPLE_FACTORS SU0011769 S00017522 Type:KO | Treatment:apyrase SUBJECT_SAMPLE_FACTORS SU0011770 S00017523 Type:KO | Treatment:apyrase SUBJECT_SAMPLE_FACTORS SU0011771 S00017524 Type:KO | Treatment:apyrase SUBJECT_SAMPLE_FACTORS SU0011772 S00017525 Type:KO | Treatment:apyrase SUBJECT_SAMPLE_FACTORS SU0011764 S00017517 Type:KO | Treatment:saline SUBJECT_SAMPLE_FACTORS SU0011765 S00017518 Type:KO | Treatment:saline SUBJECT_SAMPLE_FACTORS SU0011766 S00017519 Type:KO | Treatment:saline SUBJECT_SAMPLE_FACTORS SU0011767 S00017520 Type:KO | Treatment:saline SUBJECT_SAMPLE_FACTORS SU0011761 S00017514 Type:wild type | Treatment:apyrase SUBJECT_SAMPLE_FACTORS SU0011762 S00017515 Type:wild type | Treatment:apyrase SUBJECT_SAMPLE_FACTORS SU0011763 S00017516 Type:wild type | Treatment:apyrase SUBJECT_SAMPLE_FACTORS SU0011758 S00017511 Type:wild type | Treatment:saline SUBJECT_SAMPLE_FACTORS SU0011759 S00017512 Type:wild type | Treatment:saline SUBJECT_SAMPLE_FACTORS SU0011760 S00017513 Type:wild type | Treatment:saline #COLLECTION CO:COLLECTION_SUMMARY - CO:SAMPLE_TYPE Plasma #TREATMENT TR:TREATMENT_SUMMARY - #SAMPLEPREP SP:SAMPLEPREP_SUMMARY - SP:SAMPLEPREP_PROTOCOL_FILENAME Gly-TCA-nucleotides_analysis_protocol-2015-03-09.docx #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE HILIC CH:INSTRUMENT_NAME Agilent 1260 CH:COLUMN_NAME Phenomenex Luna NH2 (150 x 1mm, 3um) CH:METHODS_ID AQM016 CH:METHODS_FILENAME QQQ-ATP_ADP_AMP_A-update.m.zip #ANALYSIS AN:ANALYSIS_TYPE MS AN:LABORATORY_NAME MRC2 (University of Michigan) AN:ACQUISITION_PARAMETERS_FILE QQQ-ATP_ADP_AMP_A-update.m.zip #MS MS:MS_COMMENTS - MS:INSTRUMENT_NAME Agilent 6530 QTOF MS:INSTRUMENT_TYPE QTOF MS:MS_TYPE ESI MS:ION_MODE NEGATIVE #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS nM MS_METABOLITE_DATA_START Samples S00017521 S00017522 S00017523 S00017524 S00017525 S00017517 S00017518 S00017519 S00017520 S00017514 S00017515 S00017516 S00017511 S00017512 S00017513 Factors Type:KO | Treatment:apyrase Type:KO | Treatment:apyrase Type:KO | Treatment:apyrase Type:KO | Treatment:apyrase Type:KO | Treatment:apyrase Type:KO | Treatment:saline Type:KO | Treatment:saline Type:KO | Treatment:saline Type:KO | Treatment:saline Type:wild type | Treatment:apyrase Type:wild type | Treatment:apyrase Type:wild type | Treatment:apyrase Type:wild type | Treatment:saline Type:wild type | Treatment:saline Type:wild type | Treatment:saline Adenosine 1007.6439 1988.2942 679.9533 551.9662 569.2651 496.4092 431.3598 476.1889 507.5593 1037.3029 401.5582 1231.7558 654.5788 505.2897 1478.9099 ADP 678.3967 307.0083 335.1569 712.2886 914.1922 373.0058 1227.3699 593.9100 385.4795 412.2719 499.7845 1650.6618 422.9659 560.9905 1377.3388 AMP 1489.2214 468.3687 651.4922 1134.1691 1127.8465 456.9382 1312.9491 793.3782 683.6140 1581.2815 862.9433 3830.1950 997.9530 1428.6222 3966.3137 ATP 559.9511 227.4434 616.6604 941.8111 1399.1277 555.2832 1926.9506 744.4616 869.5108 248.0403 688.3181 2039.4192 501.3441 578.7499 1304.7201 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name moverz_quant ri ri_type pubchem_id inchi_key kegg_id other_id other_id_type Adenosine 60961 Adenosine UM_Target_ID ADP 6022 ADP UM_Target_ID AMP 6083 AMP UM_Target_ID ATP 5957 ATP UM_Target_ID METABOLITES_END #END