#METABOLOMICS WORKBENCH araskind_20151211_9191311_mwtab.txt DATATRACK_ID:457 efahy_20151228_102042 STUDY_ID:ST000297 ANALYSIS_ID:AN000475 PROJECT_ID:PR000238
VERSION             	1
CREATED_ON             	December 28, 2015, 10:20 am
#PROJECT
PR:PROJECT_TITLE                 	Intestinal lipid studies
PR:PROJECT_SUMMARY               	Intestinal lipid oxidation after VSG
PR:INSTITUTE                     	University of Michigan
PR:DEPARTMENT                    	Surgery
PR:LABORATORY                    	Roelofs Lab
PR:LAST_NAME                     	Roelofs
PR:FIRST_NAME                    	Karen
PR:ADDRESS                       	Ann Arbor, MI
PR:EMAIL                         	roelofs@med.umich.edu
PR:PHONE                         	734-763-8526
#STUDY
ST:STUDY_TITLE                   	Intestinal lipid oxidation after VSG
ST:STUDY_SUMMARY                 	Postprandial lipids are lower after vertical sleeve gastrectomy (VSG) surgery
ST:STUDY_SUMMARY                 	and this efect is independent of lipid absorption and chylomicron production.
ST:STUDY_SUMMARY                 	This poses a question of where the lipids are going. In order to test the
ST:STUDY_SUMMARY                 	hypothesis that intestinal oxidation of lipids is increased, Sham or VSG animals
ST:STUDY_SUMMARY                 	were gavaged with glycerol trioleate or water and sacrificed 2h later.
ST:INSTITUTE                     	University of Michigan
ST:DEPARTMENT                    	Biomedical Research Core Facilities
ST:LABORATORY                    	Metabolomics core
ST:LAST_NAME                     	Kachman
ST:FIRST_NAME                    	Maureen
ST:ADDRESS                       	6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
ST:EMAIL                         	mkachman@med.umich.edu
ST:PHONE                         	(734) 232-8175
ST:NUM_GROUPS                    	4
ST:TOTAL_SUBJECTS                	34
#SUBJECT
SU:SUBJECT_TYPE                  	Animal
SU:SUBJECT_SPECIES               	Rattus norvegicus
SU:TAXONOMY_ID                   	10114
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data
SUBJECT_SAMPLE_FACTORS           	SU0011583	S00017088	Surgery Type:Sham | Treatment:glycerol trioleate gavage	
SUBJECT_SAMPLE_FACTORS           	SU0011584	S00017089	Surgery Type:Sham | Treatment:glycerol trioleate gavage	
SUBJECT_SAMPLE_FACTORS           	SU0011585	S00017090	Surgery Type:Sham | Treatment:glycerol trioleate gavage	
SUBJECT_SAMPLE_FACTORS           	SU0011586	S00017091	Surgery Type:Sham | Treatment:glycerol trioleate gavage	
SUBJECT_SAMPLE_FACTORS           	SU0011587	S00017092	Surgery Type:Sham | Treatment:glycerol trioleate gavage	
SUBJECT_SAMPLE_FACTORS           	SU0011588	S00017093	Surgery Type:Sham | Treatment:glycerol trioleate gavage	
SUBJECT_SAMPLE_FACTORS           	SU0011589	S00017094	Surgery Type:Sham | Treatment:glycerol trioleate gavage	
SUBJECT_SAMPLE_FACTORS           	SU0011590	S00017095	Surgery Type:Sham | Treatment:glycerol trioleate gavage	
SUBJECT_SAMPLE_FACTORS           	SU0011591	S00017096	Surgery Type:Sham | Treatment:water gavage	
SUBJECT_SAMPLE_FACTORS           	SU0011592	S00017097	Surgery Type:Sham | Treatment:water gavage	
SUBJECT_SAMPLE_FACTORS           	SU0011593	S00017098	Surgery Type:Sham | Treatment:water gavage	
SUBJECT_SAMPLE_FACTORS           	SU0011594	S00017099	Surgery Type:Sham | Treatment:water gavage	
SUBJECT_SAMPLE_FACTORS           	SU0011595	S00017100	Surgery Type:Sham | Treatment:water gavage	
SUBJECT_SAMPLE_FACTORS           	SU0011596	S00017101	Surgery Type:Sham | Treatment:water gavage	
SUBJECT_SAMPLE_FACTORS           	SU0011597	S00017102	Surgery Type:Sham | Treatment:water gavage	
SUBJECT_SAMPLE_FACTORS           	SU0011598	S00017103	Surgery Type:Sham | Treatment:water gavage	
SUBJECT_SAMPLE_FACTORS           	SU0011599	S00017104	Surgery Type:Sham | Treatment:water gavage	
SUBJECT_SAMPLE_FACTORS           	SU0011600	S00017105	Surgery Type:VSG | Treatment:glycerol trioleate gavage	
SUBJECT_SAMPLE_FACTORS           	SU0011601	S00017106	Surgery Type:VSG | Treatment:glycerol trioleate gavage	
SUBJECT_SAMPLE_FACTORS           	SU0011602	S00017107	Surgery Type:VSG | Treatment:glycerol trioleate gavage	
SUBJECT_SAMPLE_FACTORS           	SU0011603	S00017108	Surgery Type:VSG | Treatment:glycerol trioleate gavage	
SUBJECT_SAMPLE_FACTORS           	SU0011604	S00017109	Surgery Type:VSG | Treatment:glycerol trioleate gavage	
SUBJECT_SAMPLE_FACTORS           	SU0011605	S00017110	Surgery Type:VSG | Treatment:glycerol trioleate gavage	
SUBJECT_SAMPLE_FACTORS           	SU0011606	S00017111	Surgery Type:VSG | Treatment:glycerol trioleate gavage	
SUBJECT_SAMPLE_FACTORS           	SU0011607	S00017112	Surgery Type:VSG | Treatment:glycerol trioleate gavage	
SUBJECT_SAMPLE_FACTORS           	SU0011608	S00017113	Surgery Type:VSG | Treatment:glycerol trioleate gavage	
SUBJECT_SAMPLE_FACTORS           	SU0011609	S00017114	Surgery Type:VSG | Treatment:water gavage	
SUBJECT_SAMPLE_FACTORS           	SU0011610	S00017115	Surgery Type:VSG | Treatment:water gavage	
SUBJECT_SAMPLE_FACTORS           	SU0011611	S00017116	Surgery Type:VSG | Treatment:water gavage	
SUBJECT_SAMPLE_FACTORS           	SU0011612	S00017117	Surgery Type:VSG | Treatment:water gavage	
SUBJECT_SAMPLE_FACTORS           	SU0011613	S00017118	Surgery Type:VSG | Treatment:water gavage	
SUBJECT_SAMPLE_FACTORS           	SU0011614	S00017119	Surgery Type:VSG | Treatment:water gavage	
SUBJECT_SAMPLE_FACTORS           	SU0011615	S00017120	Surgery Type:VSG | Treatment:water gavage	
SUBJECT_SAMPLE_FACTORS           	SU0011616	S00017121	Surgery Type:VSG | Treatment:water gavage	
#COLLECTION
CO:COLLECTION_SUMMARY            	-
CO:SAMPLE_TYPE                   	Tissues
#TREATMENT
TR:TREATMENT_SUMMARY             	-
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	-
SP:SAMPLEPREP_PROTOCOL_FILENAME  	AcylCarnitines - 20150305.docx
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_TYPE           	Reversed phase
CH:INSTRUMENT_NAME               	Agilent 1260
CH:COLUMN_NAME                   	Waters X BridgeC18 1mm x 50mm
CH:METHODS_FILENAME              	QM_004XBridge2mm_ACAR_MRM20150412.m
#ANALYSIS
AN:ANALYSIS_TYPE                 	MS
AN:LABORATORY_NAME               	MRC2 (University of Michigan)
AN:ANALYSIS_PROTOCOL_FILE        	QM_004XBridge2mm_ACAR_MRM20150412.m
#MS
MS:MS_COMMENTS                   	-
MS:INSTRUMENT_TYPE               	Triple quadrupole
MS:MS_TYPE                       	ESI
MS:ION_MODE                      	POSITIVE
MS:INSTRUMENT_NAME               	Agilent 6410 QQQ
MS:INSTRUMENT_NAME               	Agilent 6410 QQQ
#MS_METABOLITE_DATA
MS_METABOLITE_DATA:UNITS         	pmol/mg
MS_METABOLITE_DATA_START
Samples	S00017088	S00017089	S00017090	S00017091	S00017092	S00017093	S00017094	S00017095	S00017096	S00017097	S00017098	S00017099	S00017100	S00017101	S00017102	S00017103	S00017104	S00017105	S00017106	S00017107	S00017108	S00017109	S00017110	S00017111	S00017112	S00017113	S00017114	S00017115	S00017116	S00017117	S00017118	S00017119	S00017120
Factors	Surgery Type:Sham | Treatment:glycerol trioleate gavage	Surgery Type:Sham | Treatment:glycerol trioleate gavage	Surgery Type:Sham | Treatment:glycerol trioleate gavage	Surgery Type:Sham | Treatment:glycerol trioleate gavage	Surgery Type:Sham | Treatment:glycerol trioleate gavage	Surgery Type:Sham | Treatment:glycerol trioleate gavage	Surgery Type:Sham | Treatment:glycerol trioleate gavage	Surgery Type:Sham | Treatment:glycerol trioleate gavage	Surgery Type:Sham | Treatment:water gavage	Surgery Type:Sham | Treatment:water gavage	Surgery Type:Sham | Treatment:water gavage	Surgery Type:Sham | Treatment:water gavage	Surgery Type:Sham | Treatment:water gavage	Surgery Type:Sham | Treatment:water gavage	Surgery Type:Sham | Treatment:water gavage	Surgery Type:Sham | Treatment:water gavage	Surgery Type:Sham | Treatment:water gavage	Surgery Type:VSG | Treatment:glycerol trioleate gavage	Surgery Type:VSG | Treatment:glycerol trioleate gavage	Surgery Type:VSG | Treatment:glycerol trioleate gavage	Surgery Type:VSG | Treatment:glycerol trioleate gavage	Surgery Type:VSG | Treatment:glycerol trioleate gavage	Surgery Type:VSG | Treatment:glycerol trioleate gavage	Surgery Type:VSG | Treatment:glycerol trioleate gavage	Surgery Type:VSG | Treatment:glycerol trioleate gavage	Surgery Type:VSG | Treatment:glycerol trioleate gavage	Surgery Type:VSG | Treatment:water gavage	Surgery Type:VSG | Treatment:water gavage	Surgery Type:VSG | Treatment:water gavage	Surgery Type:VSG | Treatment:water gavage	Surgery Type:VSG | Treatment:water gavage	Surgery Type:VSG | Treatment:water gavage	Surgery Type:VSG | Treatment:water gavage
Carnitine	471.9	734.8	464.8	447.2	820.2	663.9	649.9	499.6	417.6	506.5	536.1	444.8	316.5	517.2	456.1	512.3	548.5	661.5	366.5	492.4	765	713.4	435.9	322.1	483.5	593.4	421.2	340.9	459.2	319.9	533.3	530.6	536.1
CAR(2:0)	222.3	341.1	214.8	186.1	371.1	322.6	287.78	322.2	183.3	195.2	181.1	285.3	137.48	244.8	148.9	253.9	191.8	254.5	116	167	417.2	327.4	108.2	180.6	199.9	227.8	138.6	113.5	199	104.9	125.8	184.5	138.5
CAR(3:0)	4.68	4.94	6.87	5.02	5.77	3.26	5.53	5.48	4.45	3.54	3.84	5.7	4.22	3.74	4.9	4.45	4.25	6.21	3.17	3.93	5.66	8.01	2.71	3.88	3.5	2.87	7.51	2.89	2.85	4.16	4.5	4.55	4.26
CAR(4:0)	11.7	14.6	10.51	10.79	13.9	12.15	14.8	15.46	13.75	13.78	11.39	17	7.48	15.28	11.84	16.66	12.61	17.19	10	11.22	14.19	21	9.22	7.25	11.83	15.45	19.13	11	11.45	8.6	10.42	20.52	12.52
CAR(5:0-DC)	0.1	0.13	0.3	0.19	0.39	0.29	0.19	0.24	0.11	0.1	0.11	0.16	0.13	0.11	0.08	0.2	0.13	0.14	0.08	0.14	0.15	0.16	0.05	0.24	0.16	0.17	0.09	0.09	0.13	0.07	0.1	0.14	0.14
CAR(5:0)	1.82	1.7	2.12	1.67	1.99	2.36	1.91	2.08	3.01	3	2.73	2.9	2.28	3.45	3.04	3.07		2.46	1.97	2.44	2.35	2.35	1.41	2.11	1.66	2.49	3.18	2.74	2.14	1.7	2.81	2.91	3.45
CAR(6:0)	2.84	2.22	1.11	1.42	1.41	1.99	1.56	1.65	2.43	0.95	0.71	1.9	0	1.16	1.47	1.74	1.81	5.29	1.84	1.15	1.67	2.49	1.12	0.77	1.44	2.52	2.82	1.26	2.01	0.99	2.03	2.7	1.62
CAR(8:1)	0.25	0.31	0.18	0.11	0.49	0.18	0.22	0.21	0.06	0.01	0.01	0.01	0.04	0.03	0.03	0.03	0.02	0.34	0.14	0.07	.0.28	0.46	0.09	0.06	0.12	0.09	0.02	0.01	0.01	0.01	0.02	0.03	0.02
CAR(8:0)	1.04	0.29	0.22	0.18	0.3	0.28	0.38	0.35	0.42	0.09	0.09	0.14	0.17	0.2	0.17	0.25	0.18	0.66	0.12	0.18	0.17	0.25	0.18	0.1	0.16	0.22	0.22	0.11	0.13	0.13	0.16	0.24	0.15
CAR(10:1)	0.17	0.18	0.1	0.05	0.28	0.37	0.14	0.12	0.09	0.05	0.08	0.08	0.1	0.09	0.07	0.1	0.08	0.18	0.11	0.07	0.09	0.18	0.12	0.04	0.1	0.19	0.08	0.04	0.09	0.06	0.18	0.08	0.1
CAR(10:0)	0.89	0.6	0.4	0.22	0.61	0.53	0.95	0.44	0.93	0.12	0.17	0.24	0.38	0.62	0.69	0.69	0.69	1.25	0.18	0.16	0.4	0.45	0.58	0.16	0.4	0.49	0.57	0.27	0.31	0.45	0.31	0.48	0.35
CAR(12:0-OH)	0.11	0.1	0.18	0.06	0.22	0.1	0.25	0.11	0.21	0.03	0.08	0.09	0.08	0.16	0.17	0.16	0.2	0.26	0.03	0.05	0.09	0.2	0.1	0.05	0.08	0.09	0.13	0.08	0.1	0.12	0.12	0.21	0.13
CAR(12:1)	0.11	0.13	0.06	0.03	0.13	0.04	0.17	0.12	0.32	0.06	0.25	0.24	0.21	0.1	0.24	0.23	0.15	0.29	0.05	0.05	0.1	0.15	0.05	0.03	0.07	0.18	0.13	0.17	0.21	0.07	0.32	0.18	0.22
CAR(14:2)	0.1	0.12	0.03	0.02	0.09	0.03	0.06	0.08	0.18	0.07	0.19	0.23	0.14	0.06	0.16	0.19	0.09	0.25	0.03	0.04	0.09	0.1	0.02	0.03	0.04	0.12	0.06	0.13	0.17	0.04	0.23	0.13	0.17
CAR(12:0)	0.79	0.66	0.71	0.27	0.63	0.37	0.95	0.66	1.67	0.26	0.81	0.83	1.02	0.85	1.31	1.57	1.15	1.21	0.23	0.26	0.46	0.68	0.56	0.27	0.49	0.7	0.9	0.76	0.55	0.59	0.98	1.16	1.09
CAR(14-OH)	0.12	0.11	0.12	0.07	0.27	0.12	0.15	0.14	0.31	0.07	0.18	0.2	0.12	0.21	0.22	0.28	0.3	0.25	0.05	0.09	0.14	0.22	0.08	0.08	0.15	0.14	0.15	0.13	0.17	0.11	0.22	0.26	0.2
CAR(14:1)	1.12	1.28	0.43	0.39	1.33	0.61	0.83	1.29	1.86	0.46	2.05	1.74	1.36	0.7	1.22	1.63	0.94	1.63	0.4	0.48	0.93	1.46	0.25	0.45	0.51	1.23	0.74	1.19	1.34	0.44	1.77	1.38	1.61
CAR(14:0)	1.12	1.2	1.06	0.84	1.58	0.86	1.13	1.63	2.25	1.02	2.15	2.74	1.68	1.29	1.8	2.6	1.89	1.52	0.77	0.87	1.07	1.86	1.08	0.96	1.15	1.31	1.63	1.48	1.23	1.06	1.47	1.66	1.78
CAR(16:0-OH)	0.38	0.3	0.28	0.22	0.84	0.44	0.48	0.29	0.82	0.24	0.29	0.51	0.23	0.86	0.56	0.74	1.02	0.53	0.11	0.19	0.46	0.39	0.26	0.14	0.79	0.39	0.39	0.34	0.48	0.23	0.5	0.5	0.5
CAR(16:1)	2.36	2.43	1.07	1.24	3.48	1.81	1.96	2.77	3.37	1.57	2.73	2.76	1.81	2.02	2.07	2.64	2.17	2.64	1.18	1.41	1.84	3.13	1.15	1.01	1.68	2.45	1.96	1.86	2.2	1.12	2.98	2.86	2.92
CAR(18:2-OH)	1.24	0.96	0.59	0.62	2.56	1.72	1.14	0.96	0.62	0.17	0.3	0.45	0.21	0.64	0.37	0.53	0.7	0.8	0.32	0.45	0.71	0.72	0.31	0.44	0.78	0.35	0.35	0.18	0.21	0.18	0.29	0.33	0.35
CAR(18:2)	1.62	1.4	0.78	0.76	1.59	1.21	1.23	1.48	0.74	0.51	0.52	0.66	0.34	0.5	0.54	0.73	0.43	0.93	0.42	0.81	1.8	0.63	0.55	0.88	0.7	1.85	0.66	0.59	1.77	0.82	1.94	1.18	0.54
CAR(20:4)	0.11	0.21	0.11	0.09	0.13	0.14	0.17	0.16	0.48	1.09	1.26	0.45	0.42	0.46	0.47	0.46	0.83	0.15	0.06	0.17	0.34	0.18	0.38	0.06	0.4	0.36	0.26	0.6	1.21	0.49	0.79	0.44	0.58
CAR(20:3)	0.04	0.01	0.02	0.03	0.03	0.04	0.04	0.04	0.03	0.06	0.07	0.05	0.03	0.02	0.03	0.02	0.06	0.05	0.02	0.02	0.02	0.02	0.01	0.04	0.02	0.03	0.03	0.03	0.04	0.03	0.03	0.02	0.03
CAR(16:0)	2.43	2.49	3.01	2.03	3.01	1.23	2.93	3.01	4.14	2.7	3.64	5.03	2.79	3.23	2.85	3.68	4.04	3.11	2.09	1.97	2.75	3.2	3	3.52	3.12	3.3	3.57	3.21	3.19	2.17	3.47	3.3	3.66
CAR(18:1)	5.52	5.03	5.2	3.96	6.59	5.54	4.81	5.81	5.72	2.93	4.03	5.97	3.22	4.2	3.94	5	4.87	5.97	4.03	3.31	4.95	5	4.31	7.07	4.71	5.43	4.63	3.83	4.15	2.51	4.96	3.91	4.72
CAR(20:2)	0.49	0.37	0.31	0.43	0.55	0.6	0.57	0.4	0.28	0.22	0.09	0.37	0.08	0.31	0.24	0.32	0.27	0.62	0.25	0.33	0.31	0.31	0.36	0.2	0.44	0.56	0.23	0.21	0.31	0.11	0.24	0.29	0.24
CAR(18:0)	2.15	2.22	3.4	2.42	2.71	2.48	2.28	3.05	3.52	3.01	3.31	4.41	2.52	2.65	2.45	3.54	3.04	2.5	11.84	1.92	2.49	1.95	3.32	3.13	3.41	3.4	3.34	3.23	3.33	2.11	3.6	2.94	3.7
CAR(20:1)	2.7	3	3.42	2.95	3.88	3.77	2.76	3.53	2.06	1.45	1.51	1.56	1.09	2.57	1.49	1.9	1.96	3.25	2.02	2.42	2.72	2.64	2.18	1.86	2.32	3.18	2.42	1.34	1.96	1.44	2.19	1.48	1.75
CAR(20:0)	0.67	0.91	0.81	0.66	1.13	1.04	0.59	0.92	0.71	0.62	0.83	0.55	0.43	1.48	0.54	0.69	1.09	0.51	0.38	0.54	0.84	0.51	0.63	0.41	0.89	0.74	0.85	0.85	0.89	0.55	1.01	0.45	0.75
MS_METABOLITE_DATA_END
#METABOLITES
METABOLITES_START
metabolite_name	PubChem CID
Carnitine	288
CAR(2:0)	1
CAR(3:0)	107738
CAR(4:0)	213144
CAR(5:0-DC)	71317118
CAR(5:0)	6426851
CAR(6:0)	3082079
CAR(8:1)	77908888
CAR(8:0)	123701
CAR(10:1)	57357170
CAR(10:0)	10245190
CAR(12:0-OH)	71464535
CAR(12:1)	87087953
CAR(14:2)	87087940
CAR(12:0)	10427569
CAR(14-OH)	71464541
CAR(14:1)	87088002
CAR(14:0)	6426854
CAR(16:0-OH)	71464553
CAR(16:1)	76040365
CAR(18:2-OH)	71464556
CAR(18:2)	53477834
CAR(20:4)	53477832
CAR(20:3)	54500892
CAR(16:0)	11953816
CAR(18:1)	6441392
CAR(20:2)	71464509
CAR(18:0)	6426855
CAR(20:1)	71464507
CAR(20:0)	53477833
METABOLITES_END
#END