#METABOLOMICS WORKBENCH michaelsa93_20151119_9939031_mwtab.txt DATATRACK_ID:432 efahy_20160115_125346 STUDY_ID:ST000318 ANALYSIS_ID:AN000505 PROJECT_ID:PR000257 VERSION 1 CREATED_ON January 15, 2016, 12:53 pm #PROJECT PR:PROJECT_TITLE Allantoin differences in Synechococcus cells grown in high versus low light PR:PROJECT_SUMMARY This experimented consisted of analysis of 500-1000ml of Synechococcus dense PR:PROJECT_SUMMARY cell cultures grown in high versus low light. The goal was to see any PR:PROJECT_SUMMARY differences in the metabolites between the two treatments, especially with PR:PROJECT_SUMMARY respect to Allantoin. PR:INSTITUTE University of California, Davis PR:DEPARTMENT Genome and Biomedical Sciences Facility PR:LABORATORY WCMC Metabolomics Core PR:LAST_NAME Fiehn PR:FIRST_NAME Oliver PR:ADDRESS 1315 Genome and Biomedical Sciences Facility, 451 Health Sciences Drive, Davis, PR:ADDRESS CA 95616 PR:EMAIL ofiehn@ucdavis.edu PR:PHONE (530) 754-8258 PR:FUNDING_SOURCE U24DK097154 #STUDY ST:STUDY_TITLE Allantoin differences in Synechococcus cells grown in high versus low ST:STUDY_TITLE lightgrowth ST:STUDY_SUMMARY This experimented consisted of analysis of 500-1000ml of Synechococcus dense ST:STUDY_SUMMARY cell cultures grown in high versus low light. The goal was to see any ST:STUDY_SUMMARY differences in the metabolites between the two treatments, especially with ST:STUDY_SUMMARY respect to Allantoin. ST:INSTITUTE University of California, Davis ST:DEPARTMENT Genome and Biomedical Sciences Facility ST:LABORATORY WCMC Metabolomics Core ST:LAST_NAME Fiehn ST:FIRST_NAME Oliver ST:ADDRESS 1315 Genome and Biomedical Sciences Facility, 451 Health Sciences Drive, Davis, ST:ADDRESS CA 95616 ST:EMAIL ofiehn@ucdavis.edu ST:PHONE (530) 754-8258 ST:NUM_GROUPS 4 ST:TOTAL_SUBJECTS 12 #SUBJECT SU:SUBJECT_TYPE Cells SU:SUBJECT_SPECIES Synechococcus sp. CC9605 #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS - 1L Light treatment:L SUBJECT_SAMPLE_FACTORS - 2L Light treatment:L SUBJECT_SAMPLE_FACTORS - 3L Light treatment:L SUBJECT_SAMPLE_FACTORS - 4D Light treatment:D SUBJECT_SAMPLE_FACTORS - 5D Light treatment:D SUBJECT_SAMPLE_FACTORS - 6D Light treatment:D #COLLECTION CO:COLLECTION_SUMMARY - CO:SAMPLE_TYPE Cells #TREATMENT TR:TREATMENT_SUMMARY Cells grown in high versus low light TR:TREATMENT_PROTOCOL_FILENAME StudyDesign_BrianPalenik_081914.pdf #SAMPLEPREP SP:SAMPLEPREP_SUMMARY 1. After quenching the cells add 1 X 106 dried cells to 1.5 ml eppendorff tube. SP:SAMPLEPREP_SUMMARY 2. Place the eppendorf tube with cells on dry ice for 20 minutes or til the SP:SAMPLEPREP_SUMMARY cells are completely frozen and ice for 20 minutes, till they are completely SP:SAMPLEPREP_SUMMARY thawed. Repeat this step twice. 3. Add 1ml of extraction solvent which has been SP:SAMPLEPREP_SUMMARY pre-chilled using the ThermoElectron Neslab RTE 740 cooling bath set to -20°C. SP:SAMPLEPREP_SUMMARY to the ependorff tube with cells. 4. Repeat step 2 twice with the extraction SP:SAMPLEPREP_SUMMARY solvent. 5. Vortex the sample for 10s and shake for 5min at 4°C using the SP:SAMPLEPREP_SUMMARY Orbital Mixing Chilling/Heating plate. Centrifuge samples for 2min at 14000 rcf SP:SAMPLEPREP_SUMMARY using the centrifuge Eppendorf 5415 D. 6. Aliquot two 500µL portions of the SP:SAMPLEPREP_SUMMARY supernatant. One for analysis and one for backup. Store one aliquot in the SP:SAMPLEPREP_SUMMARY -20°C freezer as a backup. 7. Evaporate one 500µL aliquot of the sample in the SP:SAMPLEPREP_SUMMARY Labconco Centrivap cold trap concentrator to complete dryness. . 8. Submit to SP:SAMPLEPREP_SUMMARY derivatization. SP:SAMPLEPREP_PROTOCOL_FILENAME SOP_Extraction_of_Cell_pellets.pdf #CHROMATOGRAPHY CH:CHROMATOGRAPHY_SUMMARY UPLC CH:CHROMATOGRAPHY_TYPE Reversed phase CH:INSTRUMENT_NAME Unspecified CH:COLUMN_NAME Waters Acquity CSH C18 (100 x 2.1mm, 1.7um) CH:COLUMN_NAME 1.7um Pre-Column CH:FLOW_GRADIENT 15% B to 99% B CH:FLOW_RATE 0.6 mL/min CH:COLUMN_TEMPERATURE 65 C CH:METHODS_FILENAME Data Dictionary Fiehn laboratory_CSH QTOF lipidomics_05-29-2014.pdf CH:SOLVENT_A 60:40 Acetonitrile:Water +10mM Ammonium Formate +10mM Formic Acid CH:SOLVENT_B 9:1 Isopropanol:Acetonitrile +10mM Ammonium Formate +10mM Formic Acid CH:COLUMN_PRESSURE 450-850 bar CH:INTERNAL_STANDARD See data dictionary CH:RETENTION_TIME See data dictionary CH:SAMPLE_INJECTION 1.67 uL CH:ANALYTICAL_TIME 13 min CH:CAPILLARY_VOLTAGE 3500 CH:TIME_PROGRAM 15 min CH:WEAK_WASH_SOLVENT_NAME Isopropanol CH:WEAK_WASH_VOLUME 5 seconds CH:STRONG_WASH_SOLVENT_NAME Isopropanol CH:TARGET_SAMPLE_TEMPERATURE Autosampler temp 4 C CH:RANDOMIZATION_ORDER Excel #ANALYSIS AN:ANALYSIS_TYPE MS AN:SOFTWARE_VERSION MassHunter AN:DATA_FORMAT .d #MS MS:MS_COMMENTS - MS:INSTRUMENT_NAME Agilent 6530 QTOF MS:INSTRUMENT_TYPE QTOF MS:MS_TYPE ESI MS:ION_MODE POSITIVE MS:CAPILLARY_VOLTAGE 3500 MS:COLLISION_GAS Nitrogen MS:DRY_GAS_FLOW 8 l/min MS:DRY_GAS_TEMP 325 MS:FRAGMENT_VOLTAGE 120 MS:FRAGMENTATION_METHOD Auto MS/MS MS:ION_SOURCE_TEMPERATURE 325 MS:ION_SPRAY_VOLTAGE 1000 MS:IONIZATION Pos MS:PRECURSOR_TYPE Intact Molecule MS:REAGENT_GAS Nitrogen MS:SOURCE_TEMPERATURE 325 C MS:DESOLVATION_GAS_FLOW 11 l/min MS:DESOLVATION_TEMPERATURE 350 C MS:NEBULIZER 35 psig MS:OCTPOLE_VOLTAGE 750 MS:RESOLUTION_SETTING Extended Dynamic Range MS:SCAN_RANGE_MOVERZ 60-1700 Da MS:SCANNING_CYCLE 2 Hz MS:SCANNING_RANGE 60-1700 Da MS:SKIMMER_VOLTAGE 65 #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS Peak height MS_METABOLITE_DATA_START Samples 1L 2L 3L 4D 5D 6D Factors Light treatment:L Light treatment:L Light treatment:L Light treatment:D Light treatment:D Light treatment:D PC (32:1) 3005288 3139328 1958176 1059581 2194299 665348 PC (33:2) 2974181 2699552 2581865 3174587 2852509 1576576 PC (34:1) 11773 18022 52925 6271 11347 14877 PC (34:2) 11593 81586 2083867 7989 44869 39477 PC (35:2) 169250 210295 200009 132846 201157 53249 PC (36:2) 8721 3749910 4687896 6899 1765380 2520310 PC (37:2) 40434 45415 20039 12693 PC (40:5) 14329 20470 10242 9954 SM (d34:0) 3910 3680 3202 2883 3525 2273 TG (46:0) 6226 3485 3231 4147 2212 TG (46:1) 5702 2280 2172 4076 TG (48:1) 15313 6719 5635 11305 TG (48:2) 8695 6727 TG (50:1) 17995 13525 10173 14320 5249 5217 TG (50:2) 12502 7138 5466 8854 TG (51:2) 2709 2111 TG (52:1) 8414 5633 5117 6669 TG (52:2) 11329 7816 5807 9530 4293 4029 TG (52:3) 10430 8807 5979 8769 4500 2467 TG (52:4) 2786 2692 2542 TG (54:1) 2985 2100 TG (54:2) 6598 2337 5122 TG (54:3) 18326 9991 7646 13927 5874 6964 TG (54:4) 8389 6334 4519 7070 2032 TG (54:5) 5038 5025 5047 CSH_posESI #015 9448 17889 19514 12726 10614 15525 CSH_posESI #134 2761 2165 2453 CSH_posESI #196 4605 2969 2390 3773 2433 CSH_posESI #275 3884215 3377576 2856661 2935445 3563782 2808739 CSH_posESI #285 117347 139160 47111 91631 CSH_posESI #286 3236 4208 3059 5169 14130 CSH_posESI #296 2460 842831 1190153 3003 337099 352557 0.52_138.06 44723 129594 141466 80489 95232 98422 0.81_453.17 79717 369896 327935 99031 325999 338231 0.82_459.25 37154 221670 197894 50297 204586 206450 0.82_461.27_0.82_483.25 11230 118953 94435 16712 85696 96814 0.82_281.14 4139 64698 45488 5928 40731 42940 0.82_119.09 8225 122138 85587 9961 74645 85363 0.82_135.08 3083 48012 36084 4052 31844 31120 0.82_432.24 72974 1426626 1000229 104448 884925 973237 0.82_867.37 2451 62457 47603 4057 42638 49545 0.82_473.27 52978 396175 327596 72776 308646 322909 0.82_415.21 109283 1906561 1330340 150932 1194217 1316414 0.82_446.25 4692 36307 29125 6460 27995 31467 0.82_437.19 108275 501057 461082 143913 451788 464650 0.82_846.44_0.82_851.40 7186 189076 139202 11927 120863 133162 0.86_658.47 4014 33739 16273 3703 6260 3398 0.90_288.44 482852 393710 186568 322732 0.91_332.32 95876 72282 28308 52001 8176 3515 0.90_288.29_0.91_310.27 7326901 5954675 2945358 4960222 943905 416527 0.94_244.26 343024 229281 231426 238194 66384 38437 0.95_929.50 13250 21512 7118 11768 0.96_485.27 14723 11926 5265 39631 4726 5803 0.97_304.29 38515 147031 13233 19819 23893 3806 1.00_644.39_1.00_649.34 11459 13695 7671 33354 10229 7582 1.22_523.36 5074 4952 16582 1.23_951.59 29674 27786 145093 1.23_447.30 28352 30104 14671 56377 7710 5926 1.23_487.29_1.23_465.31 402732 411136 141293 856714 74040 59549 1.23_482.33 64852 68031 30601 131552 15957 12950 1.23_303.25 100655 101259 48318 200223 23056 21060 1.23_503.26 25815 23665 10318 45947 5601 4433 1.30_316.32_1.29_338.30 4806304 6280638 6414296 5319096 1615453 1261011 1.30_316.48 318030 429255 429459 363016 96077 70469 1.30_360.35 71048 103230 67246 80294 20221 15581 1.35_272.30 281392 264779 337239 288219 98604 107988 1.36_513.30 12598 10382 5118 28489 5411 3031 1.38_532.38 16012 20030 1.39_488.36 15601 21164 1.45_653.55 15616 22207 5640 1.49_1035.64 19879 11376 10366 23932 16394 15351 1.49_1034.97 30936 17757 14897 33378 25297 24081 1.49_1552.45 6910 4201 3466 7739 5590 5048 1.50_1035.30 28107 16535 14310 32194 24118 22325 1.55_299.31_1.54_282.28 119831 535078 656354 230948 108042 121975 1.54_321.29 47073 143124 166184 77067 46686 50280 1.55_619.59 17315 100294 127681 41137 14487 19019 1.55_631.24 5927 4246 3184 8166 6937 3792 1.63_367.26 7610 28323 28275 6196 16849 9731 1.68_327.34 7614 29807 28675 14612 6418 4560 1.68_307.31 23104 50679 85962 31705 10769 7481 1.69_546.40 12575 11180 18106 17973 6125 7748 1.70_590.43 9164 8499 12700 14792 4854 6137 1.71_429.33 8645 28153 12718 15078 6142 1.71_284.33 77493 15452 5684 61237 31071 5820 1.73_502.37 12753 14330 23276 17725 7289 8758 1.73_458.35 11556 14744 27331 15717 8231 9624 1.74_414.32 7672 8607 20956 11020 4308 5712 1.79_402.36 7361 22112 7847 7771 6909 13370 1.81_393.28 28231 85664 89549 24433 53782 28255 1.90_759.54 10240 19731 14073 8757 10390 13844 1.97_644.50 16615 9314 3618 10901 2288 1.98_600.46 24687 12782 5083 16819 3143 2660 1.99_556.44 35004 18287 5745 20764 3616 2614 2.00_512.42 49359 24582 7194 28047 5095 3868 2.01_531.35 37652 5379 20380 2.02_468.39 59152 32693 8114 33180 6182 3800 2.02_424.36 58754 39556 8925 32345 7570 4774 2.04_380.34 58365 41356 9899 31043 8741 5786 2.04_963.78 5747 5482 4185 6786 2.04_964.28 6216 5635 2063 4300 6043 2.04_336.31_2.04_319.29 76153 54921 15560 41349 12424 10064 2.04_341.27 28172 21557 5694 13521 4033 3309 2.08_575.38 22178 3105 11362 2.10_327.30_2.10_349.28 41727 244454 83293 45999 50389 37529 2.16_344.32_2.17_366.30 214846 370087 113804 158648 81151 79207 2.17_709.61 57067 98896 19745 39494 13681 13399 2.18_341.32 9961 83969 25915 9348 13257 8285 2.19_936.89 6540 6065 4340 5407 6036 5027 2.20_614.46 44451 180649 22636 2.26_327.34 16423 107157 161317 28309 22972 19493 2.28_834.61 13839 20405 11059 10753 5557 3789 2.29_658.48 26431 122498 29200 14969 20492 12065 2.30_790.59 23484 34889 16527 20512 7942 5078 2.30_675.65 22343 50781 3825 2521 2144 2.32_531.27_2.29_548.30 19409 5900 6691 28818 2030 2879 2.33_702.54 61404 81689 23220 42284 16633 9489 2.35_658.51 86861 122498 29200 60038 20492 12065 2.36_614.48 129443 180649 36293 74080 30172 16163 2.37_370.33_2.37_392.31 14992 88758 26003 7239 8356 8588 2.38_570.46 172649 254212 42327 89601 40577 20460 2.39_526.43 193993 314353 44551 99643 50140 26897 2.40_609.27 9087 4408 7982 16111 3255 6754 2.42_482.41 195378 338455 46185 95741 62291 33350 2.43_545.37 47345 5196 35225 2.44_438.38_2.43_443.33 204055 348518 48864 102086 75313 44791 2.45_377.33 47328 75770 12835 26094 21139 12830 2.45_399.31_2.45_394.35 172795 260434 41071 87518 71366 44472 2.46_312.36 39348 16651 3928 10130 5057 7690 2.46_350.33_2.46_333.30 120636 182216 32911 63699 59276 39965 2.46_355.28 44176 66429 11539 23801 21651 14870 2.48_959.78 21987 12506 8788 27230 12861 10770 2.48_1279.71 18928 11637 6766 22659 11792 8576 2.48_1286.70 6682 4335 2729 8312 4519 3460 2.48_960.04 25253 14579 11141 33069 16394 12711 2.48_965.53 7386 4750 3195 8755 4359 4186 2.48_1279.38 15700 9185 6430 19657 9558 7785 2.48_1280.04 16089 9792 6577 20353 10421 7136 2.48_960.29_2.48_965.28 29138 16487 12146 35218 17139 14536 2.50_589.39 56492 5881 40148 2.58_430.39 26564 32344 10605 25080 9596 19650 2.58_433.31 14501 31206 37162 12009 28391 11994 2.59_543.40 3475 11692 9206 2924 4902 5423 2.60_508.42 83442 10493 2821 51163 2.61_464.40 55551 6133 36353 2.62_633.42 38254 23806 29169 2.62_980.70 6943 13273 9547 4840 4560 3823 2.63_499.37 11456 22863 17072 7408 8667 7910 2.63_1024.58 45885 6833 8751 2.63_975.47 4585 6041 2883 12268 2.63_654.33_2.63_659.29 693678 809507 676024 855262 448607 251935 2.63_972.99_2.63_977.94 9053 10052 5005 21996 2.63_388.28 42612 9282 6110 13776 2.63_681.34 60107 58145 41601 73359 27058 16779 2.63_695.36 119974 123287 84900 134968 43230 25305 2.63_1290.63_2.64_1295.59 47547 55593 26629 104618 7963 2.64_727.28 7568 5933 4392 31950 2894 2.64_477.36 13696 27457 20646 8909 10115 8729 2.64_936.68 17062 27098 16562 11772 8406 6695 2.65_629.48 35951 288331 2602 26787 2.66_892.65 34538 54446 32932 21491 14504 11657 2.66_455.35 16311 31935 21250 9416 10184 8490 2.68_848.63 60802 106891 50911 36469 26832 19132 2.70_804.60 106221 184498 73684 57922 41898 27876 2.70_677.45 15381 18032 3679 10382 3176 2256 2.71_760.58 156969 289776 89199 82131 58719 38244 2.73_673.50 105389 215730 44245 18472 39478 24739 2.73_717.55 84263 160106 41076 43031 30728 19571 2.75_672.52 288396 554922 125249 140977 107103 65175 2.77_628.49_2.77_633.45 373237 802453 140025 179504 139853 84542 2.79_589.43 12772 30805 4370 5690 5372 3586 2.79_780.49 33451 101748 37636 14257 36171 51572 2.81_540.45_2.81_545.40 430757 1034821 147133 225121 189285 120644 2.83_496.42_2.83_501.38 369261 905876 133948 212350 193909 131127 2.84_457.35_2.84_435.37 75175 174799 26866 50151 42209 32880 2.85_796.49_2.85_801.44 55559 227585 63254 28604 73362 153965 2.85_452.39 306361 588200 99917 204741 170940 120421 2.86_391.34 81820 113783 22399 58506 36472 30517 2.86_413.32_2.86_408.37 368120 501577 97175 255255 163197 128515 2.88_369.30 84479 104857 26234 60864 41604 34968 2.88_364.34_2.88_347.32 253557 318430 70924 181124 117778 102156 2.88_616.45 3111 9229 6462 2183 3609 4906 2.89_832.60 6491 13516 12217 2847 4528 4263 2.89_594.43 5395 15140 10720 3107 5193 8346 2.89_325.27 28674 50845 16264 23562 22780 22720 2.90_788.57 14490 24974 17592 8399 6982 6594 2.90_572.42 10286 24930 17393 5862 8531 11666 2.90_823.43 9235 20418 5414 6395 5709 13107 2.91_550.41 15672 39755 27849 9965 14346 16791 2.92_700.53 40680 59634 34728 26472 14721 12651 2.92_1038.75 9350 21775 14906 4496 6592 7835 2.93_528.39 26836 57078 42212 13784 20183 22324 2.93_656.51 43586 81107 40983 31257 18278 17024 2.94_506.38 41025 76781 53636 21164 27620 27191 2.94_994.73 21764 46950 29719 9862 14237 13908 2.94_355.33_2.94_377.31 21934 107247 98884 25385 25690 22906 2.94_612.47_2.94_617.43 60545 133635 34372 39008 28438 28642 2.95_806.50 18821 40641 15137 8669 13537 21356 2.95_573.40 4967 18819 4590 3157 3838 4103 2.95_484.37 56446 92316 68868 26897 35235 31340 2.95_950.70 47587 92035 56971 20566 27421 25447 2.95_529.38 6360 26104 9512 3787 5760 6254 2.96_524.42 26929 153556 24233 22533 31006 38206 2.96_462.35 62389 96299 59900 28894 37431 30951 2.96_906.67 98635 170600 95777 42928 51596 45069 2.97_485.36_2.98_480.39 35008 164104 32684 27357 35897 47933 2.98_440.34 33010 47125 27579 15228 19009 14258 2.98_862.65 179385 310784 163137 75604 98821 74732 2.98_822.50_2.98_827.46 28490 63061 21474 15969 22536 44503 2.99_372.35_2.99_394.33 144497 143638 71971 140774 39520 51144 2.99_436.36_2.99_441.32 30276 117479 25312 27212 26451 36931 2.99_818.62 291400 492561 215707 118332 157818 115342 3.01_774.59 393036 674842 250946 154183 218334 152501 3.01_779.54 13477 26192 8765 5286 7323 5787 3.02_1460.12 3968 4007 2723 5914 3.02_730.54 489615 972780 301036 187916 289486 206346 3.04_686.54 553612 1312145 315416 212860 350246 251690 3.05_1191.84 70969 81824 102457 71561 109179 63685 3.05_577.28 21375 12853 16639 38611 25995 50109 3.05_642.51_3.05_647.47 561194 1632350 324683 254421 415124 300611 3.05_587.44 31675 55873 79881 26828 46730 20299 3.06_545.46 52555 62963 39486 22980 24511 30684 3.06_585.43 361825 587177 851582 297460 522382 220389 3.07_599.45 213419 603059 112425 115429 149207 109984 3.07_603.44 21592 72470 12597 11022 14996 12042 3.07_625.48 4173 10696 5899 2751 3109 2830 3.09_607.41 13741 12932 18535 16109 19644 13439 3.09_1448.12 14685 10831 8786 23443 17328 15360 3.09_1455.45 9727 7511 5725 16013 11495 11455 3.09_733.47 127324 186115 94540 64738 71683 120111 3.09_1447.79 16199 11540 10530 25711 17660 18658 3.09_1455.12 10788 7730 6337 16551 11965 12363 3.09_711.46 36785 119835 26236 10107 27124 47507 3.09_554.46_3.09_559.42 615498 1687401 300586 435867 432736 332728 3.09_1447.46 11701 9757 7995 20896 13072 13241 3.09_1454.78 7962 5248 4665 13797 9795 8846 3.10_653.45 11805 8234 10735 12318 10341 8735 3.10_493.40 11984 39437 9924 9585 11659 8902 3.10_510.44_3.10_515.39 416422 1338957 260551 319970 391063 326190 3.11_507.41 14691 56087 12280 6529 21450 32801 3.11_704.50_3.11_709.45 132560 515790 98681 44087 132787 298028 3.12_466.41_3.12_449.38 397436 898425 221605 372708 318188 264611 3.12_471.37 69346 166893 34613 59714 51351 47473 3.12_623.41 40898 44014 69582 33320 34947 27878 3.14_422.38_3.14_405.36 647699 686138 213726 636667 270980 238188 3.14_427.34 123318 134836 39767 114349 49731 48269 3.13_383.31_3.15_378.36 544957 429268 164656 511450 184327 176573 3.15_361.33 141134 111756 43532 135772 48109 44777 3.15_419.38 13537 10957 5608 12219 5410 5247 3.17_745.54 3991 13955 2894 2579 2419 5577 3.17_334.33 23594 30464 16665 22158 16318 15220 3.17_517.42 10974 19234 7483 4211 6101 8321 3.17_626.49 5994 9359 3640 4945 3630 4643 3.18_778.48 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5.24_773.59 28148 24346 63911 11869 26229 8189 5.24_763.54 17282 15534 18790 9782 11365 3503 5.26_785.58 38269 111317 18278 5.26_977.53 5768 7236 10258 4038 2827 5.30_784.57 2971 74715 232697 2782 25939 38274 5.38_840.59_5.37_845.54 203009 258019 265448 187717 153789 135186 5.37_744.56 6680 15639 18085 3433 3937 2455 5.38_741.49 69773 72433 68662 59339 71900 49279 5.38_1548.07_5.38_1570.06 10138 13149 11096 7496 8915 5.38_761.59 31880 30031 29674 25841 31665 17794 5.39_742.53 31339 31084 144130 25721 31004 21020 5.39_725.51 1835090 1958158 1781131 1351442 1829685 947761 5.39_793.51 33055 34061 28426 25157 34576 16921 5.40_591.53 8129 21610 2352 6813 3671 5.40_721.56_5.39_726.52 1703670 2079693 1680819 1044170 1575933 669768 5.40_1428.05 221445 309246 228424 85296 195046 36684 5.40_742.50 31339 31084 144130 25721 31004 21020 5.40_523.47 291712 380379 290730 139763 254580 76350 5.40_541.48 91486 120721 89483 45046 78112 23406 5.40_286.25 34233 42469 30544 17944 30042 9887 5.42_866.60 4914 71657 80736 4076 41301 49954 5.43_1454.06 133019 180872 157199 65138 109484 27369 5.44_746.58 1974571 3251445 3314265 1312716 1611574 588752 5.44_339.29 34450 37625 28732 22355 30176 12139 5.44_313.27 168612 242515 246958 89367 142490 45645 5.44_819.52 36758 39118 37902 32496 34321 20044 5.44_767.51 69084 69030 69701 65228 70961 47455 5.47_549.49 120216 203046 203606 77389 100176 34513 5.47_963.67 10456 10646 8396 6684 7663 9333 5.47_1480.08_5.47_1502.06 172281 296532 314288 102172 126284 28003 5.48_768.54 30355 32620 31884 29033 30193 21175 5.49_731.59 4485 6323 10653 3268 3397 5.49_751.54 1735749 2036091 2032845 1476786 1687337 888551 5.50_1506.09 9315 13599 12890 5471 9084 5.52_866.55 4914 9426 11962 4076 41301 6542 5.52_787.60 31295 1886679 2360036 28823 884602 1273733 5.52_949.58_5.53_971.56 18899 26112 19996 23439 31162 23317 5.54_1235.84 92923 54274 82049 97022 36116 48138 5.54_910.65_5.54_915.60 511494 337137 253641 329444 311449 135852 5.57_773.56 51634 64436 63911 38005 9605 15290 5.58_810.58 3389 86823 109930 3033 38998 60317 5.60_936.66_5.60_941.61 21643 18056 13803 16578 16355 7451 5.63_768.57 9360 11524 31884 9936 5112 8990 5.64_844.65 20130 23631 9053 14656 5.66_824.56 16312 20723 12538 5.66_1099.91 19883 22836 24509 3583 6164 5625 5.67_556.49_5.67_561.45 137640 155833 147802 53919 83449 56971 5.67_814.63 83839 111510 58704 56270 5.67_577.42 16935 17201 16139 9046 11113 10752 5.70_605.55 58318 59576 30850 34804 5.70_576.49 16258 10937 8465 14202 4391 5539 5.70_965.60 7712 15146 11657 7849 9923 4925 5.70_794.59 14021 13290 6606 7397 5.73_734.58_5.73_739.52 234438 454313 219630 59981 255210 34239 5.73_755.51 6965 12175 6676 2177 8067 5.74_587.47 39984 33687 30185 11832 14336 5408 5.74_536.61 12205 2723 2756 2470 2150 5.79_591.42 7558 3718 4169 6443 9577 4569 5.81_1159.85 2043 2434 2755 7726 5.82_679.60 17655 20534 8258 12246 5.83_945.66 12388 11762 5185 5.85_705.61_5.85_727.60 450560 560455 155175 260139 5.93_1096.05 6847 29086 13908 5138 5.94_595.59 30432 67610 43349 23714 8885 10053 5.95_538.54 93401 189715 119088 75741 27381 27672 5.98_551.43 22777 35547 57712 23682 38203 17807 5.98_1199.98 5355 10561 6601 4610 5.99_451.36 26203 30698 25579 28405 36681 17868 6.00_1011.64_6.01_1033.62 25320 42439 35156 27295 34842 12817 6.02_1631.04 8751 8906 8802 8225 7543 4068 6.03_812.60 2108 3021 3041 2350 2157 6.03_1572.99_6.03_1594.97 104633 150068 126062 119372 89907 48518 6.04_1417.00 11529 8253 12715 9024 5841 8034 6.04_1415.99 13564 10137 12990 8825 6121 9950 6.05_769.52 19274 15943 17958 12493 14930 11472 6.06_1484.08 8107 8107 7804 8592 5049 3672 6.06_1554.98 12149 12848 12362 11797 8059 5693 6.06_1386.79 2692 5298 3837 4087 4559 6.06_748.59_6.07_753.55 908778 980932 993220 672947 794932 395617 6.07_341.31 27041 32553 29703 21029 26398 10753 6.07_999.51 23161 21814 23411 25506 26493 21556 6.07_569.51 20926 21607 22853 18368 21426 7067 6.07_1641.10_6.08_1663.08 93098 140204 120814 74425 95810 21362 6.08_910.54 587449 1006653 779908 730542 775642 367555 6.08_967.59 6236 5385 5835 6620 7408 5848 6.08_1067.49 6552 5147 5864 7666 6549 6243 6.08_911.54 337512 550643 436753 405127 444477 196572 6.10_976.52 2794 4689 3868 3522 2782 2754 6.10_613.23 7931 12346 9611 9304 8783 4341 6.10_908.53 36242 78141 54650 44296 37615 28121 6.10_931.52 352894 410411 384122 416378 420594 242621 6.10_551.51 59212 69143 64579 43834 50906 23293 6.11_891.53 575919 1001953 757364 711946 703180 343999 6.11_1689.10 7695 6860 6654 6950 7595 6.13_774.61_6.13_779.57 92693 59577 66218 74252 78808 35688 6.14_928.54 27079 45981 33621 19555 40082 9811 6.15_925.55 249501 506471 340236 249470 379558 109750 6.16_693.61 11457 17013 12759 8769 6.18_923.56_6.17_945.54 644522 1281683 805518 922965 976295 357700 6.18_1013.52 32242 45234 36803 42268 42273 20064 6.18_924.55 204841 541608 278330 316020 334508 121409 6.19_1081.51 3748 4849 4302 5543 4949 3018 6.19_981.61 12237 12389 11857 14358 14673 6811 6.19_789.62 2904 16264 18925 2031 8601 21137 6.20_921.54 80687 125457 97654 79555 106876 58269 6.24_563.47 23364 24351 41419 12379 12215 10181 6.25_1103.94 8704 13328 27122 2497 2994 6.25_947.67 10553 37862 22949 5222 26455 16164 6.26_1487.01 4934 11520 8044 3201 4207 6.27_1457.00 12215 9069 24278 5916 5674 6182 6.28_951.60 14961 8698 14976 12166 11883 12546 6.30_915.53 197721 116355 275711 274678 215073 333204 6.30_918.54 28490 16332 39238 39528 30902 44906 6.30_1051.50 5567 3742 5671 6243 5444 6799 6.30_983.53 16163 10350 17440 17863 15846 19835 6.30_894.54 1057215 758076 1950719 1940387 1275326 2530627 6.30_615.24 6010 4753 11439 10937 7283 14051 6.31_1483.02 8685 4123 10137 3236 6.35_684.20 2027 7938 8265 6.36_1529.02 6448 3939 7657 2396 6.38_762.60 7304 10323 7484 4612 9120 2140 6.38_629.22 8688 10044 8912 8958 9944 7211 6.39_907.52 186340 220755 194628 177633 211699 154522 6.39_940.56 2129351 2427625 2203090 2080070 2380262 1820060 6.39_938.54 107024 155697 127698 104363 122957 102532 6.40_975.71 5264 10382 9135 2478 8912 8633 6.42_865.51 12652 16244 31506 10294 23945 5646 6.42_961.53 445514 489430 448773 454858 503511 418523 6.42_965.56 18325 21973 18993 18724 22019 17530 6.43_957.56 9522 12983 11238 9602 11243 8951 6.43_1097.50 7368 7198 6718 6996 7054 7562 6.43_1007.53_6.43_1029.52 36062 39517 36238 35652 39285 36554 6.43_968.74_6.44_973.70 16044 37657 31743 8768 29770 30133 6.45_977.50 20527 19393 18788 19874 22565 14632 6.45_997.60 21106 20661 20871 21300 22760 16635 6.48_551.43 11618 17152 14534 11879 16202 10471 6.48_987.58 8956 16607 8495 7485 19126 6142 6.49_979.54 23614 55164 25809 17072 50459 12414 6.49_980.59 14697 34247 16738 10635 31078 8068 6.57_577.48 16430 10241 7569 11255 3880 6.61_983.51 8324 8815 6803 8977 7639 4551 6.61_896.55 49613 55678 44647 54672 38230 22582 6.63_1459.11 3706 8736 4590 3830 6.66_709.59 14413 10444 16047 6088 6.72_1010.72 6980 8455 6469 5181 4220 3156 6.73_1159.85 2996 2977 6068 2447 5728 6.74_781.58 9691 8972 9488 7402 6206 3176 6.87_992.71 3786 6958 5666 2241 5928 3960 6.90_970.76_6.90_975.71 8050 26776 26267 4800 16703 17246 6.94_1018.72 6034 7938 7375 3447 6696 3181 6.96_607.47 13323 18656 25893 10740 9612 9053 6.97_1160.00 30507 66156 188374 13781 10900 10175 6.97_591.50 66618 89907 186349 46730 42267 28522 6.97_659.48 3536 6173 14588 2502 2024 7.00_1143.93 3796 4789 6516 3014 3384 2127 7.00_554.44 50016 82186 103072 50934 65087 34578 7.12_887.57 482451 512182 379151 568793 324106 259266 7.12_910.55 127214 119038 101060 142528 94498 65439 7.13_925.53 17092 20974 17836 19131 15428 11828 7.14_903.57 68381 113787 90837 61703 62207 30772 7.29_918.58 26912 42422 33884 27880 34744 20989 7.34_917.58 42961 70655 52749 43876 56041 45391 7.39_944.74_7.38_949.70 90463 79227 63644 51961 33826 23269 7.39_994.72 5616 12404 14086 4637 8208 10883 7.39_1486.15 2573 3076 5609 4827 5035 7.39_1487.15 3055 3656 6385 4688 2022 5013 7.43_871.57 183197 135992 464674 441081 227801 607585 7.44_1020.74_7.44_1025.69 22377 36574 34798 17338 33312 24105 7.47_901.58_7.48_923.57 138388 276813 153784 208297 172547 76365 7.48_1046.75 6021 7975 6090 4425 5970 3857 7.64_619.53 12560 13133 32431 9036 4538 3571 7.67_1090.80 6513 5295 4205 4502 6692 2450 7.72_926.73_7.71_931.69 19545 24802 18994 12539 19358 12317 7.93_1022.75 6337 10236 10199 4322 6965 6874 7.95_594.48 7141 14923 10962 7784 11614 7471 7.99_1048.77 6915 9691 7635 5805 7950 5080 8.02_671.55 19909 17772 14325 28344 23527 26005 8.03_650.62_8.03_655.58 55581 44703 34807 97429 58660 63490 8.15_928.75 35460 65228 49809 24283 51924 23917 8.22_933.70 33266 62683 47982 19209 41640 24768 8.24_1118.83_8.24_1123.79 9457 10459 7905 6219 13876 2710 8.27_954.76_8.27_959.72 105520 128182 100833 58177 103263 50381 8.47_1050.79 7607 10071 12673 5022 5626 8277 8.47_1120.84 6685 5872 5103 8.49_1128.83 17106 16313 9517 8452 8.50_947.72 3951 7302 5563 2193 4474 3108 8.52_444.37 8368 8108 9204 7641 7619 8303 8.53_581.44 27534 27198 32528 21366 20788 25686 8.53_553.44 19387 18713 20389 15279 18099 18873 8.54_1056.82 29480 22422 8184 11721 8.55_1061.78 13210 9392 4211 8.69_956.78 35265 64968 57119 19521 54791 21792 8.72_957.78 21782 39601 35254 12522 32408 14658 8.78_961.73 38193 62078 58837 21216 46467 24887 8.79_982.79 4111 5511 5630 2335 4221 2138 9.08_1211.80 16087 5168 3205 9.13_1211.79 16087 5621 3205 9.46_921.75 7561 15568 28332 4967 9887 12220 9.47_787.58 55116 47366 35995 56771 54834 51201 9.48_1218.79 5371 7224 6371 5248 3147 2803 9.49_1229.77 4959 5040 5166 4694 2581 2793 9.49_766.65_9.50_771.61 312064 266807 168453 319395 223909 201510 9.53_885.67_9.52_880.72 219153 427952 122996 159936 9.53_863.69 27318 49349 14426 16919 9.53_901.65 21307 38194 16065 20531 9.54_907.73 10589 20390 8179 9330 10.12_1277.35 6005 5245 8797 11165 4294 10.63_869.70 2969 2151 10.69_1426.39 4784 5549 6712 8341 3195 10.95_1500.41 3530 4466 5232 6393 2594 11.00_923.75 4355 2133 3581 2391 11.18_1574.43 3121 3837 4291 5757 2124 11.87_908.86 9428 5621 6115 7104 5504 5658 12.02_936.89 9090 3424 2902 6176 12.07_950.91 8281 2342 2508 6102 12.10_964.93 6522 2364 2377 4405 12.17_1286.06 6771 4887 6468 3630 7360 7191 12.17_1287.06 6559 4792 6185 3898 7838 6934 12.65_146.17 64935 80676 74640 37200 34789 33129 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name Identifier m/z RT Adduct InChI Key Molecular Formula PC (32:1) 5.12_732.56_5.15_737.52 732.5592_737.5176 5.134 [M+H]+ QIBZFHLFHCIUOT-NPBIGWJUSA-N C40H78NO8P PC (33:2) 4.91_744.56_4.94_749.52 744.5599_749.5206 4.925 [M+H]+ SBNDHGBVMZMSNL-UESLNCBNSA-N C41H78NO8P PC (34:1) 5.70_760.59 760.5882 5.704 [M+H]+ WTJKGGKOPKCXLL-VYOBOKEXSA-N C42H82NO8P PC (34:2) 5.09_758.57 758.5716 5.087 [M+H]+ JLPULHDHAOZNQI-ZTIMHPMXSA-N C42H80NO8P PC (35:2) 5.51_772.59_5.52_777.55 772.5906_777.5503 5.5145 [M+H]+ LNGBVAOHJZCRIL-GPDPEMMZSA-N C43H82NO8P PC (36:2) 5.65_786.60 786.6013 5.651 [M+H]+ SNKAWJBJQDLSFF-NVKMUCNASA-N C44H84NO8P PC (37:2) 5.86_800.62 800.6166 5.858 [M+H]+ MCZUABDVGPPWPM-HJTCUGKVSA-N C45H86NO8P PC (40:5) 5.66_836.61 836.6147 5.658 [M+H]+ UCKDHIBTSOLZAW-OMGITNNSSA-N C48H86NO8P SM (d34:0) 4.92_705.58 705.5836 4.923 [M+H]+ QHZIGNLCLJPLCU-QPPIDDCLSA-N C39H81N2O6P TG (46:0) 10.53_796.74 796.7406 10.528 [M+NH4]+ JWVXCFSNEOMSHH-UHFFFAOYSA-N C49H94O6 TG (46:1) 10.12_799.68 799.6789 10.122 [M+Na]+ RSDIQTNCDWUNDP-VHXPQNKSSA-N C49H92O6 TG (48:1) 10.57_827.71_10.58_822.76 827.7104_822.7560 10.575 [M+Na]+_[M+NH4]+ FEKLSEFRUGWUOS-DLOIZKPKSA-N C51H96O6 TG (48:2) 10.18_820.74_10.18_825.70 820.7406_825.6952 10.182 [M+NH4]+_[M+Na]+ RUOVJPPUXXFZPC-YZEIBMOJSA-N C51H94O6 TG (50:1) 10.97_855.74_10.98_850.79 855.7424_850.7866 10.974 [M+Na]+_[M+NH4]+ YHMDGPZOSGBQRH-YYSBDVFPSA-N C53H100O6 TG (50:2) 10.59_853.73_10.60_848.77 853.7268_848.7715 10.597 [M+Na]+_[M+NH4]+ QEZWFCZNHWUARW-XQCAQTCHSA-N C53H98O6 TG (51:2) 10.78_867.74 867.7424 10.782 [M+Na]+ NSNSZGBCOIKUBU-SZOKBDNISA-N C54H100O6 TG (52:1) 11.35_878.82_11.35_883.77 878.8179_883.7729 11.351 [M+NH4]+_[M+Na]+ NPCZZYKITFKRQZ-RFBIWTDZSA-N C55H104O6 TG (52:2) 10.98_881.76 881.7575 10.98 [M+Na]+ JFISYPWOVQNHLS-NBHCHVEOSA-N C55H102O6 TG (52:3) 10.62_874.79_10.63_879.74 874.7877_879.7424 10.621 [M+NH4]+_[M+Na]+ KGLAHZTWGPHKFF-FBSASISJSA-N C55H100O6 TG (52:4) 10.24_877.73 877.7276 10.243 [M+Na]+ WHSWXEYWNPTUPW-HNJDVRDNSA-N C55H98O6 TG (54:1) 11.72_911.80 911.8032 11.722 [M+Na]+ YFFIQXNTTVSKJC-NZEOUKRFSA-N C57H108O6 TG (54:2) 11.36_909.79_11.37_904.83 909.7889_904.8336 11.3615 [M+Na]+_[M+NH4]+ RYNHWWNZNIGDAQ-WGSDILPMSA-N C57H106O6 TG (54:3) 10.99_902.82_11.00_907.77 902.8172_907.7736 10.9965 [M+NH4]+_[M+Na]+ PHYFQTYBJUILEZ-IUPFWZBJSA-N C57H104O6 TG (54:4) 10.64_900.80_10.65_905.76 900.8011_905.7572 10.6465 [M+NH4]+_[M+Na]+ BRLGHZXETDWABO-NOFIOOQLSA-N C57H102O6 TG (54:5) 10.27_898.79_10.28_903.74 898.7859_903.7427 10.275 [M+NH4]+_[M+Na]+ VVEBTVMJPTZDHO-YQFZQLSNSA-N C57H100O6 CSH_posESI #015 3.48_279.16 279.16 3.476 CSH_posESI #134 10.64_895.72 895.7176 10.639 CSH_posESI #196 10.98_897.73 897.7323 10.98 CSH_posESI #275 4.91_882.61_4.92_887.57 882.6149_887.5694 4.9125 CSH_posESI #285 5.64_876.57 876.5711 5.643 CSH_posESI #286 5.25_1159.85 1159.8455 5.25 CSH_posESI #296 5.13_603.54 603.5353 5.127 0.52_138.06 0.52_138.06 138.0553 0.516 0.81_453.17 0.81_453.17 453.1681 0.815 0.82_459.25 0.82_459.25 459.2497 0.816 0.82_461.27_0.82_483.25 0.82_461.27_0.82_483.25 461.2663_483.2497 0.817 0.82_281.14 0.82_281.14 281.1387 0.817 0.82_119.09 0.82_119.09 119.0856 0.818 0.82_135.08 0.82_135.08 135.0803 0.818 0.82_432.24 0.82_432.24 432.2389 0.819 0.82_867.37 0.82_867.37 867.3717 0.819 0.82_473.27 0.82_473.27 473.2651 0.819 0.82_415.21 0.82_415.21 415.2124 0.82 0.82_446.25 0.82_446.25 446.2544 0.82 0.82_437.19 0.82_437.19 437.1942 0.821 0.82_846.44_0.82_851.40 0.82_846.44_0.82_851.40 846.4418_851.3980 0.821 0.86_658.47 0.86_658.47 658.4678 0.865 0.90_288.44 0.90_288.44 288.4431 0.9 0.91_332.32 0.91_332.32 332.3164 0.907 0.90_288.29_0.91_310.27 0.90_288.29_0.91_310.27 288.2900_310.2715 0.9075 0.94_244.26 0.94_244.26 244.2638 0.944 0.95_929.50 0.95_929.50 929.5028 0.95 0.96_485.27 0.96_485.27 485.2723 0.961 0.97_304.29 0.97_304.29 304.2864 0.966 1.00_644.39_1.00_649.34 1.00_644.39_1.00_649.34 644.3860_649.3414 1.003 1.22_523.36 1.22_523.36 523.3591 1.224 1.23_951.59 1.23_951.59 951.5869 1.226 1.23_447.30 1.23_447.30 447.2958 1.23 1.23_487.29_1.23_465.31 1.23_487.29_1.23_465.31 487.2885_465.3062 1.2305 1.23_482.33 1.23_482.33 482.3329 1.232 1.23_303.25 1.23_303.25 303.2535 1.232 1.23_503.26 1.23_503.26 503.2626 1.234 1.30_316.32_1.29_338.30 1.30_316.32_1.29_338.30 316.3213_338.3030 1.2945 1.30_316.48 1.30_316.48 316.4826 1.296 1.30_360.35 1.30_360.35 360.3474 1.3 1.35_272.30 1.35_272.30 272.295 1.35 1.36_513.30 1.36_513.30 513.3039 1.357 1.38_532.38 1.38_532.38 532.3845 1.382 1.39_488.36 1.39_488.36 488.3578 1.388 1.45_653.55 1.45_653.55 653.5458 1.448 1.49_1035.64 1.49_1035.64 1035.637 1.494 1.49_1034.97 1.49_1034.97 1034.969 1.495 1.49_1552.45 1.49_1552.45 1552.4481 1.495 1.50_1035.30 1.50_1035.30 1035.303 1.498 1.55_299.31_1.54_282.28 1.55_299.31_1.54_282.28 299.3063_282.2846 1.5435 1.54_321.29 1.54_321.29 321.288 1.545 1.55_619.59 1.55_619.59 619.5867 1.546 1.55_631.24 1.55_631.24 631.2402 1.548 1.63_367.26 1.63_367.26 367.2634 1.627 1.68_327.34 1.68_327.34 327.3375 1.676 1.68_307.31 1.68_307.31 307.3111 1.681 1.69_546.40 1.69_546.40 546.3999 1.689 1.70_590.43 1.70_590.43 590.4263 1.705 1.71_429.33 1.71_429.33 429.3325 1.71 1.71_284.33 1.71_284.33 284.3313 1.71 1.73_502.37 1.73_502.37 502.3743 1.726 1.73_458.35 1.73_458.35 458.3477 1.73 1.74_414.32 1.74_414.32 414.3214 1.737 1.79_402.36 1.79_402.36 402.3581 1.788 1.81_393.28 1.81_393.28 393.2796 1.813 1.90_759.54 1.90_759.54 759.537 1.904 1.97_644.50 1.97_644.50 644.495 1.965 1.98_600.46 1.98_600.46 600.465 1.983 1.99_556.44 1.99_556.44 556.4423 1.992 2.00_512.42 2.00_512.42 512.4152 1.996 2.01_531.35 2.01_531.35 531.3509 2.006 2.02_468.39 2.02_468.39 468.3899 2.018 2.02_424.36 2.02_424.36 424.3638 2.024 2.04_380.34 2.04_380.34 380.3374 2.035 2.04_963.78 2.04_963.78 963.7835 2.037 2.04_964.28 2.04_964.28 964.2843 2.038 2.04_336.31_2.04_319.29 2.04_336.31_2.04_319.29 336.3115_319.2852 2.044 2.04_341.27 2.04_341.27 341.2665 2.045 2.08_575.38 2.08_575.38 575.3774 2.075 2.10_327.30_2.10_349.28 2.10_327.30_2.10_349.28 327.3012_349.2830 2.104 2.16_344.32_2.17_366.30 2.16_344.32_2.17_366.30 344.3164_366.2983 2.1665 2.17_709.61 2.17_709.61 709.6074 2.168 2.18_341.32 2.18_341.32 341.3165 2.183 2.19_936.89 2.19_936.89 936.8858 2.194 2.20_614.46 2.20_614.46 614.4567 2.205 2.26_327.34 2.26_327.34 327.3372 2.264 2.28_834.61 2.28_834.61 834.6128 2.28 2.29_658.48 2.29_658.48 658.484 2.287 2.30_790.59 2.30_790.59 790.5858 2.296 2.30_675.65 2.30_675.65 675.6488 2.305 2.32_531.27_2.29_548.30 2.32_531.27_2.29_548.30 531.2749_548.3008 2.308 2.33_702.54 2.33_702.54 702.5358 2.329 2.35_658.51 2.35_658.51 658.5103 2.346 2.36_614.48 2.36_614.48 614.484 2.362 2.37_370.33_2.37_392.31 2.37_370.33_2.37_392.31 370.3325_392.3143 2.365 2.38_570.46 2.38_570.46 570.4582 2.382 2.39_526.43 2.39_526.43 526.4295 2.393 2.40_609.27 2.40_609.27 609.2711 2.396 2.42_482.41 2.42_482.41 482.4056 2.418 2.43_545.37 2.43_545.37 545.3664 2.432 2.44_438.38_2.43_443.33 2.44_438.38_2.43_443.33 438.3793_443.3345 2.4355 2.45_377.33 2.45_377.33 377.3261 2.448 2.45_399.31_2.45_394.35 2.45_399.31_2.45_394.35 399.3089_394.3527 2.449 2.46_312.36 2.46_312.36 312.3628 2.457 2.46_350.33_2.46_333.30 2.46_350.33_2.46_333.30 350.3252_333.3006 2.4605 2.46_355.28 2.46_355.28 355.2836 2.463 2.48_959.78 2.48_959.78 959.7847 2.479 2.48_1279.71 2.48_1279.71 1279.7093 2.479 2.48_1286.70 2.48_1286.70 1286.7024 2.479 2.48_960.04 2.48_960.04 960.0351 2.48 2.48_965.53 2.48_965.53 965.531 2.481 2.48_1279.38 2.48_1279.38 1279.3753 2.481 2.48_1280.04 2.48_1280.04 1280.043 2.483 2.48_960.29_2.48_965.28 2.48_960.29_2.48_965.28 960.2862_965.2799 2.484 2.50_589.39 2.50_589.39 589.3931 2.504 2.58_430.39 2.58_430.39 430.3894 2.575 2.58_433.31 2.58_433.31 433.3136 2.577 2.59_543.40 2.59_543.40 543.3989 2.589 2.60_508.42 2.60_508.42 508.421 2.595 2.61_464.40 2.61_464.40 464.3953 2.605 2.62_633.42 2.62_633.42 633.4242 2.622 2.62_980.70 2.62_980.70 980.7049 2.625 2.63_499.37 2.63_499.37 499.3716 2.628 2.63_1024.58 2.63_1024.58 1024.5822 2.63 2.63_975.47 2.63_975.47 975.4654 2.631 2.63_654.33_2.63_659.29 2.63_654.33_2.63_659.29 654.3331_659.2883 2.6315 2.63_972.99_2.63_977.94 2.63_972.99_2.63_977.94 972.9867_977.9404 2.633 2.63_388.28 2.63_388.28 388.282 2.633 2.63_681.34 2.63_681.34 681.3439 2.634 2.63_695.36 2.63_695.36 695.3602 2.634 2.63_1290.63_2.64_1295.59 2.63_1290.63_2.64_1295.59 1290.6311_1295.5872 2.635 2.64_727.28 2.64_727.28 727.2763 2.64 2.64_477.36 2.64_477.36 477.3593 2.643 2.64_936.68 2.64_936.68 936.678 2.643 2.65_629.48 2.65_629.48 629.4782 2.645 2.66_892.65 2.66_892.65 892.6519 2.658 2.66_455.35 2.66_455.35 455.3467 2.664 2.68_848.63 2.68_848.63 848.6282 2.678 2.70_804.60 2.70_804.60 804.6009 2.698 2.70_677.45 2.70_677.45 677.4495 2.703 2.71_760.58 2.71_760.58 760.5754 2.715 2.73_673.50 2.73_673.50 673.5045 2.726 2.73_717.55 2.73_717.55 717.5515 2.732 2.75_672.52 2.75_672.52 672.5222 2.755 2.77_628.49_2.77_633.45 2.77_628.49_2.77_633.45 628.4936_633.4542 2.7705 2.79_589.43 2.79_589.43 589.4285 2.791 2.79_780.49 2.79_780.49 780.4862 2.791 2.81_540.45_2.81_545.40 2.81_540.45_2.81_545.40 540.4474_545.4030 2.8085 2.83_496.42_2.83_501.38 2.83_496.42_2.83_501.38 496.4211_501.3763 2.828 2.84_457.35_2.84_435.37 2.84_457.35_2.84_435.37 457.3503_435.3683 2.8435 2.85_796.49_2.85_801.44 2.85_796.49_2.85_801.44 796.4889_801.4443 2.8455 2.85_452.39 2.85_452.39 452.395 2.846 2.86_391.34 2.86_391.34 391.3424 2.861 2.86_413.32_2.86_408.37 2.86_413.32_2.86_408.37 413.3244_408.3683 2.863 2.88_369.30 2.88_369.30 369.2978 2.877 2.88_364.34_2.88_347.32 2.88_364.34_2.88_347.32 364.3424_347.316 2.878 2.88_616.45 2.88_616.45 616.4451 2.878 2.89_832.60 2.89_832.60 832.602 2.886 2.89_594.43 2.89_594.43 594.434 2.888 2.89_325.27 2.89_325.27 325.2716 2.892 2.90_788.57 2.90_788.57 788.575 2.896 2.90_572.42 2.90_572.42 572.4207 2.9 2.90_823.43 2.90_823.43 823.4344 2.904 2.91_550.41 2.91_550.41 550.4079 2.914 2.92_700.53 2.92_700.53 700.5275 2.922 2.92_1038.75 2.92_1038.75 1038.7512 2.925 2.93_528.39 2.93_528.39 528.3941 2.926 2.93_656.51 2.93_656.51 656.506 2.932 2.94_506.38 2.94_506.38 506.3815 2.938 2.94_994.73 2.94_994.73 994.7262 2.938 2.94_355.33_2.94_377.31 2.94_355.33_2.94_377.31 355.3320_377.3144 2.9425 2.94_612.47_2.94_617.43 2.94_612.47_2.94_617.43 612.4724_617.4267 2.9435 2.95_806.50 2.95_806.50 806.5043 2.946 2.95_573.40 2.95_573.40 573.4004 2.946 2.95_484.37 2.95_484.37 484.3682 2.951 2.95_950.70 2.95_950.70 950.6992 2.951 2.95_529.38 2.95_529.38 529.3816 2.953 2.96_524.42 2.96_524.42 524.4205 2.964 2.96_462.35 2.96_462.35 462.355 2.965 2.96_906.67 2.96_906.67 906.6728 2.965 2.97_485.36_2.98_480.39 2.97_485.36_2.98_480.39 485.3562_480.3942 2.9725 2.98_440.34 2.98_440.34 440.3416 2.979 2.98_862.65 2.98_862.65 862.6464 2.98 2.98_822.50_2.98_827.46 2.98_822.50_2.98_827.46 822.5043_827.4596 2.98 2.99_372.35_2.99_394.33 2.99_372.35_2.99_394.33 372.3475_394.3297 2.987 2.99_436.36_2.99_441.32 2.99_436.36_2.99_441.32 436.3621_441.3247 2.988 2.99_818.62 2.99_818.62 818.62 2.993 3.01_774.59 3.01_774.59 774.593 3.006 3.01_779.54 3.01_779.54 779.539 3.009 3.02_1460.12 3.02_1460.12 1460.1152 3.019 3.02_730.54 3.02_730.54 730.5402 3.022 3.04_686.54 3.04_686.54 686.5411 3.038 3.05_1191.84 3.05_1191.84 1191.8363 3.047 3.05_577.28 3.05_577.28 577.2818 3.049 3.05_642.51_3.05_647.47 3.05_642.51_3.05_647.47 642.5148_647.4714 3.053 3.05_587.44 3.05_587.44 587.4376 3.055 3.06_545.46 3.06_545.46 545.4566 3.063 3.06_585.43 3.06_585.43 585.4311 3.064 3.07_599.45 3.07_599.45 599.4451 3.07 3.07_603.44 3.07_603.44 603.4431 3.07 3.07_625.48 3.07_625.48 625.4777 3.073 3.09_607.41 3.09_607.41 607.4131 3.085 3.09_1448.12 3.09_1448.12 1448.1232 3.087 3.09_1455.45 3.09_1455.45 1455.4518 3.087 3.09_733.47 3.09_733.47 733.4743 3.087 3.09_1447.79 3.09_1447.79 1447.7895 3.087 3.09_1455.12 3.09_1455.12 1455.1166 3.087 3.09_711.46 3.09_711.46 711.4645 3.088 3.09_554.46_3.09_559.42 3.09_554.46_3.09_559.42 554.4624_559.4189 3.088 3.09_1447.46 3.09_1447.46 1447.4557 3.088 3.09_1454.78 3.09_1454.78 1454.7839 3.088 3.10_653.45 3.10_653.45 653.4538 3.098 3.10_493.40 3.10_493.40 493.3963 3.101 3.10_510.44_3.10_515.39 3.10_510.44_3.10_515.39 510.4369_515.3923 3.105 3.11_507.41 3.11_507.41 507.4055 3.106 3.11_704.50_3.11_709.45 3.11_704.50_3.11_709.45 704.4954_709.4512 3.11 3.12_466.41_3.12_449.38 3.12_466.41_3.12_449.38 466.4106_449.3843 3.121 3.12_471.37 3.12_471.37 471.3661 3.121 3.12_623.41 3.12_623.41 623.4086 3.121 3.14_422.38_3.14_405.36 3.14_422.38_3.14_405.36 422.3836_405.3582 3.138 3.14_427.34 3.14_427.34 427.3392 3.138 3.13_383.31_3.15_378.36 3.13_383.31_3.15_378.36 383.31_378.3581 3.1445 3.15_361.33 3.15_361.33 361.3318 3.154 3.15_419.38 3.15_419.38 419.3827 3.154 3.17_745.54 3.17_745.54 745.5367 3.167 3.17_334.33 3.17_334.33 334.3319 3.171 3.17_517.42 3.17_517.42 517.4249 3.175 3.17_626.49 3.17_626.49 626.4863 3.175 3.18_778.48 3.18_778.48 778.4794 3.184 3.18_713.48 3.18_713.48 713.4833 3.185 3.19_533.42 3.19_533.42 533.4207 3.186 3.19_735.49 3.19_735.49 735.4864 3.186 3.19_803.46 3.19_803.46 803.4561 3.189 3.20_894.58_3.19_899.54 3.20_894.58_3.19_899.54 894.5793_899.5354 3.1955 3.20_601.43 3.20_601.43 601.4291 3.197 3.20_582.46 3.20_582.46 582.4581 3.2 3.20_670.31_3.20_675.27 3.20_670.31_3.20_675.27 670.3096_675.2655 3.2035 3.21_808.52 3.21_808.52 808.5151 3.207 3.23_1223.82 3.23_1223.82 1223.8238 3.232 3.23_851.46_3.23_829.48 3.23_851.46_3.23_829.48 851.4608_829.4756 3.234 3.23_1207.83 3.23_1207.83 1207.8315 3.235 3.24_824.52 3.24_824.52 824.5197 3.236 3.24_753.47 3.24_753.47 753.4736 3.24 3.37_436.40 3.37_436.40 436.3986 3.367 3.39_464.39 3.39_464.39 464.3922 3.386 3.39_1344.35 3.39_1344.35 1344.3487 3.392 3.39_1339.75 3.39_1339.75 1339.7533 3.393 3.40_1339.95 3.40_1339.95 1339.9522 3.401 3.41_775.56 3.41_775.56 775.5627 3.41 3.41_569.44 3.41_569.44 569.4357 3.414 3.41_551.43 3.41_551.43 551.4265 3.414 3.41_627.48 3.41_627.48 627.4788 3.414 3.41_477.37 3.41_477.37 477.3731 3.414 3.41_681.49 3.41_681.49 681.494 3.414 3.42_568.43 3.42_568.43 568.4295 3.415 3.42_570.44 3.42_570.44 570.4398 3.415 3.42_761.54 3.42_761.54 761.5447 3.416 3.42_717.52_3.41_739.49 3.42_717.52_3.41_739.49 717.5193_739.4945 3.4165 3.42_285.24 3.42_285.24 285.2423 3.417 3.42_699.50 3.42_699.50 699.5007 3.417 3.42_519.44 3.42_519.44 519.441 3.417 3.42_309.24 3.42_309.24 309.2425 3.418 3.42_613.43 3.42_613.43 613.4266 3.418 3.43_420.37 3.43_420.37 420.369 3.429 3.44_754.47 3.44_754.47 754.4742 3.436 3.44_836.55 3.44_836.55 836.5509 3.442 3.45_553.45 3.45_553.45 553.4457 3.446 3.45_325.24 3.45_325.24 325.238 3.447 3.45_535.44 3.45_535.44 535.4365 3.449 3.45_738.49 3.45_738.49 738.4857 3.45 3.46_732.53 3.46_732.53 732.5263 3.457 3.46_773.55 3.46_773.55 773.5532 3.458 3.46_805.48 3.46_805.48 805.4765 3.46 3.46_759.54 3.46_759.54 759.5395 3.461 3.46_836.54 3.46_836.54 836.5369 3.461 3.47_826.53 3.47_826.53 826.5341 3.469 3.48_391.28 3.48_391.28 391.2849 3.476 3.48_261.15 3.48_261.15 261.1493 3.477 3.48_149.02 3.48_149.02 149.0235 3.478 3.48_852.55 3.48_852.55 852.5512 3.485 3.50_371.32 3.50_371.32 371.3162 3.503 3.51_563.47 3.51_563.47 563.4662 3.507 3.52_1336.55 3.52_1336.55 1336.5534 3.518 3.52_850.55 3.52_850.55 850.5526 3.518 3.52_774.56 3.52_774.56 774.5585 3.519 3.52_783.51 3.52_783.51 783.5129 3.523 3.53_780.49_3.53_785.45 3.53_780.49_3.53_785.45 780.4941_785.4509 3.528 3.53_561.45 3.53_561.45 561.4523 3.531 3.53_1569.89 3.53_1569.89 1569.8877 3.531 3.53_763.50 3.53_763.50 763.4982 3.533 3.54_547.40 3.54_547.40 547.4013 3.54 3.56_799.57 3.56_799.57 799.5666 3.561 3.59_592.43 3.59_592.43 592.4338 3.591 3.59_806.51 3.59_806.51 806.51 3.594 3.60_573.42 3.60_573.42 573.4169 3.601 3.61_833.45 3.61_833.45 833.4484 3.613 3.62_922.61 3.62_922.61 922.6097 3.617 3.62_768.51 3.62_768.51 768.5058 3.624 3.63_769.50 3.63_769.50 769.4975 3.626 3.63_1241.62_3.63_1258.64 3.63_1241.62_3.63_1258.64 1241.6152_1258.6416 3.628 3.64_876.57 3.64_876.57 876.5685 3.636 3.66_751.50 3.66_751.50 751.4976 3.656 3.66_1361.32 3.66_1361.32 1361.3168 3.661 3.68_351.27 3.68_351.27 351.2681 3.681 3.70_794.51_3.70_799.46 3.70_794.51_3.70_799.46 794.5099_799.4646 3.699 3.70_1245.47 3.70_1245.47 1245.4724 3.699 3.72_1241.22 3.72_1241.22 1241.2245 3.715 3.72_1241.47 3.72_1241.47 1241.4748 3.715 3.72_992.98 3.72_992.98 992.9812 3.717 3.72_1252.47 3.72_1252.47 1252.4655 3.717 3.72_1246.72 3.72_1246.72 1246.7199 3.718 3.72_1246.97 3.72_1246.97 1246.97 3.718 3.72_1252.21 3.72_1252.21 1252.2149 3.719 3.75_734.53 3.75_734.53 734.5338 3.754 3.75_756.50_3.76_761.45 3.75_756.50_3.76_761.45 756.4972_761.4508 3.755 3.76_1494.95_3.76_1499.91 3.76_1494.95_3.76_1499.91 1494.9523_1499.9092 3.762 3.78_667.44 3.78_667.44 667.4402 3.776 3.78_789.50 3.78_789.50 789.5048 3.782 3.79_1520.97_3.79_1525.92 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