#METABOLOMICS WORKBENCH michaelsa93_20160115_9104631_mwtab.txt DATATRACK_ID:486 kenanazam_20160122_141113 STUDY_ID:ST000339 ANALYSIS_ID:AN000550 PROJECT_ID:PR000271 VERSION 1 CREATED_ON January 22, 2016, 2:58 pm #PROJECT PR:PROJECT_TITLE Metabolites in peritoneal macrophages and bone marrow derived macrophages PR:PROJECT_SUMMARY "Experiment looks to identify metabolites in the major metabolic pathways, i.e. PR:PROJECT_SUMMARY glycolysis, tca, urea cycle and ppp along with amino acids." PR:INSTITUTE University of California, Davis PR:DEPARTMENT Genome and Biomedical Sciences Facility PR:LABORATORY WCMC Metabolomics Core PR:LAST_NAME Fiehn PR:FIRST_NAME Oliver PR:ADDRESS 1315 Genome and Biomedical Sciences Facility, 451 Health Sciences Drive, Davis, PR:ADDRESS CA 95616 PR:EMAIL ofiehn@ucdavis.edu PR:PHONE (530) 754-8258 PR:FUNDING_SOURCE NIH U24DK097154 #STUDY ST:STUDY_TITLE Metabolites in peritoneal macrophages and bone marrow derived macrophages ST:STUDY_SUMMARY "Experiment looks to identify metabolites in the major metabolic pathways, i.e. ST:STUDY_SUMMARY glycolysis, tca, urea cycle and ppp along with amino acids." ST:INSTITUTE University of California, Davis ST:DEPARTMENT Genome and Biomedical Sciences Facility ST:LABORATORY WCMC Metabolomics Core ST:LAST_NAME Fiehn ST:FIRST_NAME Oliver ST:ADDRESS 1315 Genome and Biomedical Sciences Facility, 451 Health Sciences Drive, Davis, ST:ADDRESS CA 95616 ST:EMAIL ofiehn@ucdavis.edu ST:PHONE (530) 754-8258 ST:NUM_GROUPS 4 ST:TOTAL_SUBJECTS 24 #SUBJECT SU:SUBJECT_TYPE Animal SU:SUBJECT_SPECIES Mus musculus SU:TAXONOMY_ID 10090 SU:SUBJECT_COMMENTS cells #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS Resident macrophage 1 Quigley_Inj12_SA001 Treatment:Resident macrophage Organ=Cells; Species=Mouse SUBJECT_SAMPLE_FACTORS Resident macrophage 2 Quigley_Inj03_SA002 Treatment:Resident macrophage Organ=Cells; Species=Mouse SUBJECT_SAMPLE_FACTORS Resident macrophage 3 Quigley_Inj09_SA003 Treatment:Resident macrophage Organ=Cells; Species=Mouse SUBJECT_SAMPLE_FACTORS Resident macrophage 4 Quigley_Inj08_SA004 Treatment:Resident macrophage Organ=Cells; Species=Mouse SUBJECT_SAMPLE_FACTORS Resident macrophage 5 Quigley_Inj11_SA005 Treatment:Resident macrophage Organ=Cells; Species=Mouse SUBJECT_SAMPLE_FACTORS Resident macrophage 6 Quigley_Inj05_SA006 Treatment:Resident macrophage Organ=Cells; Species=Mouse SUBJECT_SAMPLE_FACTORS Bone marrow macrophage 1 Quigley_Inj06_SA007 Treatment:Bone marrow macrophage Organ=Cells; Species=Mouse SUBJECT_SAMPLE_FACTORS Bone marrow macrophage 2 Quigley_Inj07_SA008 Treatment:Bone marrow macrophage Organ=Cells; Species=Mouse SUBJECT_SAMPLE_FACTORS Bone marrow macrophage 3 Quigley_Inj10_SA009 Treatment:Bone marrow macrophage Organ=Cells; Species=Mouse SUBJECT_SAMPLE_FACTORS Bone marrow macrophage 4 Quigley_Inj13_SA010 Treatment:Bone marrow macrophage Organ=Cells; Species=Mouse SUBJECT_SAMPLE_FACTORS Bone marrow macrophage 5 Quigley_Inj14_SA011 Treatment:Bone marrow macrophage Organ=Cells; Species=Mouse SUBJECT_SAMPLE_FACTORS Bone marrow macrophage 6 Quigley_Inj04_SA012 Treatment:Bone marrow macrophage Organ=Cells; Species=Mouse #COLLECTION CO:COLLECTION_SUMMARY "Cells were centrifuged at 500g in PBS, the supernatant aspirated and the cell CO:COLLECTION_SUMMARY pellet snap frozen in liquid nitrogen" CO:COLLECTION_PROTOCOL_FILENAME StudyDesign-LauraQuigley-121214.pdf CO:SAMPLE_TYPE Cell #TREATMENT TR:TREATMENT_SUMMARY "Different cell types: 1. Peritoneal macrophages 2. Bone marrow derived TR:TREATMENT_SUMMARY macrophages" TR:TREATMENT_PROTOCOL_FILENAME StudyDesign-LauraQuigley-121214.pdf #SAMPLEPREP SP:SAMPLEPREP_SUMMARY "1. Add 0.5mL of extraction solvent to tube, gently pipet to remove all cells, SP:SAMPLEPREP_SUMMARY transfer cells to 2mL eppendorf tube. Repeat for a total of 1mL extraction SP:SAMPLEPREP_SUMMARY solvent + cells in 2mL eppendorf tube. 2. Add 2 small stainless steel grinding SP:SAMPLEPREP_SUMMARY beads to eppendorf tube 3. Use the GenoGrinder to grind for 3 minutes at 1,250 SP:SAMPLEPREP_SUMMARY rpm. 4. Centrifuge at 14,000xg for 5 minutes. 5. Transfer supernatant to a fresh SP:SAMPLEPREP_SUMMARY 2mL eppendorf tube. 6. Add 1mL of extraction solvent to tube containing cell SP:SAMPLEPREP_SUMMARY pellet + beads, and repeat steps 3 and 4. 7. Collect supernatant, and combine SP:SAMPLEPREP_SUMMARY with supernatant collected in step 5. Total volume of extracted sample will be SP:SAMPLEPREP_SUMMARY approximately 2mL. 8. Dry down 50uL of extracted sample in 1.5mL eppendorf tube SP:SAMPLEPREP_SUMMARY for GC-TOF analysis. 9. Store backups in -20 or -80C." SP:SAMPLEPREP_PROTOCOL_FILENAME SOP_Extraction_of_Yeast_Cells.pdf #CHROMATOGRAPHY CH:CHROMATOGRAPHY_SUMMARY UPLC CH:CHROMATOGRAPHY_TYPE Reversed phase CH:INSTRUMENT_NAME Agilent 6530 CH:COLUMN_NAME Waters Acquity CSH C18 (100 x 2.1mm, 1.7um) CH:COLUMN_NAME 1.7um Pre-Column CH:FLOW_GRADIENT 15% B to 99% B CH:FLOW_RATE 0.6 mL/min CH:COLUMN_TEMPERATURE 65 C CH:METHODS_FILENAME Data Dictionary Fiehn laboratory_CSH QTOF lipidomics_05-29-2014.pdf CH:SOLVENT_A 60:40 Acetonitrile:Water +10mM Ammonium Formate +10mM Formic Acid CH:SOLVENT_B 9:1 Isopropanol:Acetonitrile +10mM Ammonium Formate +10mM Formic Acid CH:COLUMN_PRESSURE 450-850 bar CH:INTERNAL_STANDARD See data dictionary CH:RETENTION_TIME See data dictionary CH:SAMPLE_INJECTION 1.67 uL CH:ANALYTICAL_TIME 13 min CH:CAPILLARY_VOLTAGE 3500 CH:TIME_PROGRAM 15 min CH:WEAK_WASH_SOLVENT_NAME Isopropanol CH:WEAK_WASH_VOLUME 5 seconds CH:STRONG_WASH_SOLVENT_NAME same CH:TARGET_SAMPLE_TEMPERATURE Autosampler temp 4 C CH:RANDOMIZATION_ORDER Excel #ANALYSIS AN:ANALYSIS_TYPE MS AN:DETECTOR_TYPE TOF MCP AN:SOFTWARE_VERSION MassHunter AN:DATA_FORMAT .d #MS MS:INSTRUMENT_NAME Agilent 6530 QTOF MS:INSTRUMENT_TYPE QTOF MS:MS_TYPE ESI MS:ION_MODE POSITIVE MS:MS_COMMENTS Lipidomics runs are MS1 data; Identifications made from pooled MS/MS runs MS:MS_COMMENTS Lipidomics runs are performed in negative and positve mode (two filenames) MS:DRY_GAS_FLOW 8 l/min MS:DRY_GAS_TEMP 325 MS:FRAGMENT_VOLTAGE 120 MS:FRAGMENTATION_METHOD Auto MS/MS MS:ION_SOURCE_TEMPERATURE 325 MS:ION_SPRAY_VOLTAGE 1000 MS:IONIZATION Pos MS:PRECURSOR_TYPE Intact Molecule MS:REAGENT_GAS Nitrogen MS:SOURCE_TEMPERATURE 325 MS:DESOLVATION_GAS_FLOW 11 l/min MS:DESOLVATION_TEMPERATURE 350 MS:NEBULIZER 35 psig MS:OCTPOLE_VOLTAGE 750 MS:RESOLUTION_SETTING Extended Dynamic Range MS:SCAN_RANGE_MOVERZ 60-1700 Da MS:SCANNING_CYCLE 2 Hz MS:SCANNING_RANGE 60-1700 Da MS:SKIMMER_VOLTAGE 65 #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS peak height MS_METABOLITE_DATA_START Samples Quigley_Inj12_SA001 Quigley_Inj03_SA002 Quigley_Inj09_SA003 Quigley_Inj08_SA004 Quigley_Inj11_SA005 Quigley_Inj05_SA006 Quigley_Inj06_SA007 Quigley_Inj07_SA008 Quigley_Inj10_SA009 Quigley_Inj13_SA010 Quigley_Inj14_SA011 Quigley_Inj04_SA012 Factors Treatment:Resident macrophage Treatment:Resident macrophage Treatment:Resident macrophage Treatment:Resident macrophage Treatment:Resident macrophage Treatment:Resident macrophage Treatment:Bone marrow macrophage Treatment:Bone marrow macrophage Treatment:Bone marrow macrophage Treatment:Bone marrow macrophage Treatment:Bone marrow macrophage Treatment:Bone marrow macrophage CE (18:1) 6568 13106 7139 12237 5370 6395 CE (20:4) 44059 53391 48004 48177 34916 41437 CE (22:6) 4646 4455 4167 2621 3773 Ceramide (d18:1/23:0) 2326 8547 2254 5043 2444 2475 6226 11394 4728 16378 11682 Ceramide (d40:1) 3041 13338 2460 6446 2615 2955 36650 23739 44672 18592 56839 44269 Ceramide (d42:1) 9762 40953 9855 21316 10342 3796 84808 45857 87297 35414 143350 92946 Ceramide (d42:2) 45649 118885 46146 65716 47096 54560 119103 62341 122301 48210 137842 111003 Cholesterol 47910 54762 48432 48829 49636 48690 33980 15584 34902 14446 31486 33759 Gal-Gal-Cer(d18:1/16:0) or Lactosylceramide(d18:1/16:0) 3361 3720 2663 2794 3083 3509 15262 7669 13656 6131 10269 14116 GlcCer (d40:1) 5651 21245 5462 9537 5859 6420 56983 28244 50616 21968 77679 52921 GlcCer (d42:1) 10748 57687 10320 25872 11218 13578 62538 37442 68441 28320 122341 72515 GlcCer (d42:2) 18141 68829 16166 29070 17830 19787 24754 12485 24031 10868 32105 24291 LPC (16:0) 35300 38408 27223 31905 35880 37366 11137 5531 11907 4352 8893 9588 LPC (18:0) 45015 51751 38541 39645 45139 45945 12592 6389 12674 5309 10385 11045 LPC (18:1) 3120 3215 2488 2847 3053 3699 2156 1201 2169 864 1779 1957 PC (28:0) 10537 4625 9131 3869 7130 8333 PC (30:0) 157699 188921 130300 127718 150188 161562 867421 406537 777161 333011 611210 736542 PC (30:1) 11390 5604 10164 4109 8161 9245 PC (31:0) 27267 33142 25280 24529 26252 28641 240472 118165 213665 95507 167740 206739 PC (32:0) 2007299 2354472 1801955 1742434 1968502 2088035 3003290 1439351 2813402 1219461 2302467 2655055 PC (32:1) 125337 145986 101971 97888 110956 124080 392554 183548 345702 150472 274513 333649 PC (33:0) 21775 28437 20008 20487 22555 24208 127655 64273 112271 51218 86510 106501 PC (33:1) 11571 14004 10501 10263 11538 11503 130703 63492 111062 52494 91476 110918 PC (34:0) 261173 349513 232228 224821 270300 286561 1108096 554985 1018357 449974 802996 944057 PC (34:1) 1257177 1466862 1117567 1075363 1257773 1359932 2617784 1207315 2351998 1022913 1953824 2260384 PC (34:2) 920491 1087525 843918 797640 977492 1031498 338773 160536 296691 133273 240753 284391 PC (34:3) 8046 10018 6995 6651 7337 9685 14840 6892 12514 5868 11099 13292 PC (34:4) 3779 4384 3345 2894 3509 3843 32752 15539 26867 12062 21458 27282 PC (35:1) 18192 23978 17502 16746 19674 21613 123262 60504 114459 49420 87482 107638 PC (36:1) 292963 378274 258224 251500 299654 315515 623664 304017 591048 252847 470537 556187 PC (36:2) 660487 801431 613187 596002 723291 778794 510069 233995 468646 203541 385496 439704 PC (36:3) 294017 348818 251182 248817 291078 300172 242329 51350 94823 42472 76110 88649 PC (36:4) 2000322 2436690 1812344 1754336 1926388 2032660 2132340 1028831 1963645 854666 1551021 1869515 PC (36:5) 10541 12291 8110 7988 9012 10304 51275 26303 46661 20717 36058 44215 PC (38:2) 54327 74491 48310 48182 57615 61486 28969 14320 26998 11747 21175 24153 PC (38:3) 198879 246121 176568 172581 196313 208293 63656 29917 56161 26240 44130 52253 PC (38:4) 6262 8616 5798 6235 6766 6345 24646 11756 22733 9720 17591 21067 PC (38:5) 425645 509187 369548 354763 410684 428014 913361 432237 809226 363530 657010 793652 PC (38:6) 320011 370537 271349 260328 308878 331863 303386 150295 284287 121920 223603 257468 PC (40:4) 148016 184294 135619 131854 135647 150895 237244 7076 191405 92075 40916 196398 PC (40:5) 110926 132344 104763 97078 106635 120184 222747 109768 200301 88140 161541 195686 PC (40:6) 148418 175969 132524 129238 148363 158073 111658 52036 103728 44368 83811 97750 PC (40:7) 77095 88358 61583 60756 72417 77616 127489 58510 119537 49314 94740 106404 PC (40:8) 31053 37478 27890 26455 30969 32406 58222 30940 53251 23824 41612 53329 PC (42:5) 6158 7766 5492 5283 5420 5795 18349 8769 15125 6874 12484 15286 PC (42:6) 6335 7041 6100 5547 6454 6540 10421 4761 8865 5326 7011 8617 PC (42:7) 5129 6899 4255 4637 4321 4948 10064 4846 9308 4021 7378 8787 PC (o-32:0) 134201 163996 120430 114643 135294 144485 668763 351649 681716 278050 539239 589126 PC (o-34:0) 21711 32749 18034 18272 19667 23423 95471 49000 91655 39113 78253 83784 PC (p-34:0) or PC (o-34:1) 190895 237650 168277 165648 193492 209530 519910 251873 493017 210481 392748 461759 PC (p-34:1) or PC (o-34:2) 53121 63580 49040 47277 62003 64704 40137 18159 38962 15557 33302 33460 PC (p-36:1) or PC (o-36:2) 24701 31469 22728 21557 26750 29399 47343 23641 45079 19540 37224 40574 PC (p-36:3) or PC (o-36:4) 658845 798869 616522 592015 676656 714480 1968391 987518 1871093 810174 1539927 1745540 PC (p-36:4) or PC (o-36:5) 151497 180289 143918 141982 146438 152627 284043 145554 257661 117068 195471 243188 PC (p-38:3) or PC (o-38:4) 86611 111071 77267 75866 85730 90816 513759 261651 496532 212034 413264 455349 PC (p-38:4) or PC (o-38:5) 702333 825727 657786 639285 724182 736632 2015935 980413 1919760 831284 1612577 1843643 PC (p-38:5) or PC (o-38:6) 63664 78142 55631 52772 62648 68520 210904 107885 199987 85912 159809 187471 PC (p-40:3) or PC (o-40:4) 11744 16089 10923 11122 12404 13849 48086 26527 44078 20385 38571 41712 PC (p-40:4) or PC (o-40:5) 52133 64363 50212 47022 56363 59896 201935 104584 182099 83835 143532 173483 PC (p-40:6) or PC (o-40:7) 64382 124171 56808 54759 62151 69132 249329 126514 227392 104892 179857 216023 PC (p-42:3) or PC (o-42:4) 13288 7573 13775 5495 14129 12994 PC (p-42:4) or PC (o-42:5) 4065 5678 3831 3780 4446 4695 13087 6386 10896 5127 9606 11024 PC (p-42:5) or PC (o-42:6) 2839 3896 2885 2722 3024 3345 11842 6063 11818 4804 9090 10478 PE (38:6) 26484 32323 20434 21202 26021 27885 28607 14641 27691 12343 20941 25950 PE (p-36:4) or PE (o-36:5) 417021 521261 374741 356441 411912 437226 613136 312132 600913 251305 460781 554388 PE (p-38:4) or PE (o-38:5) 287322 461195 254630 265381 267618 291583 402066 215064 394706 170964 316293 358982 PE (p-38:5) or PE (o-38:6) 118081 147200 107787 100217 114708 122708 318688 158711 299459 130066 244264 283937 PE (p-40:5) or PE (o-40:6) 17388 30554 15386 16462 17884 19462 82732 44275 78579 35046 63602 73897 SM (d32:1) 3360 4027 3391 2938 3261 3629 23594 11275 21713 9154 17293 19615 SM (d33:1) 30070 38575 30212 28456 31650 34404 55960 27653 51165 21547 41123 46768 SM (d34:1) 2358861 2783589 2079962 2044838 2275963 2402946 3346369 1599779 3142107 1355313 2613425 2936187 SM (d34:2) 41414 49057 32453 32613 37571 41139 28076 13908 26521 10885 21249 23546 SM (d36:1) 38559 48443 36834 34568 39239 41284 228371 114474 221574 94834 182610 213249 SM (d38:1) 12037 16848 11649 11770 11444 12865 102612 51388 99113 40791 76510 87262 SM (d39:1) 5169 2367 2660 2681 11074 5581 9777 4547 8392 9209 SM (d40:1) 46959 85118 42926 48719 45628 51513 649203 324207 607741 259390 514573 575094 SM (d41:1) 17675 46920 17832 24624 17438 19416 51493 25250 49525 21287 55626 46357 SM (d41:2) 10290 19086 10565 11273 9699 10921 70633 34571 65137 28429 56075 60527 SM (d42:1) 56659 185988 55541 90206 55347 62183 451766 225193 442406 180076 478913 397234 SM (d42:2) 547646 877416 503890 550516 518448 551805 1189168 580371 1099037 476587 908047 1021201 SM (d42:3) 146704 210280 130566 131118 141658 156730 329551 161737 295101 131590 237492 286851 SM (d43:2) 2802 9845 5004 9530 4043 9984 8852 TG (14:0/14:0/14:0) 6859 3256 4006 18232 6201 850 2944 1982 TG (46:0) 4236 8889 5144 6012 3223 15381 5218 9469 3561 2620 5051 3871 TG (48:0) 19787 33924 20841 22342 17184 41767 20416 38664 18599 19638 19447 17837 TG (48:1) 7396 17401 4669 8181 4455 7232 6476 4547 3195 2980 4616 3398 TG (48:2) 3368 9081 2223 4134 3182 2168 1346 1890 TG (49:0) 3965 2651 3036 4109 2795 3141 3241 2844 TG (50:0) 19978 27448 18880 19129 16923 22389 28527 69907 31117 58827 30589 30148 TG (50:1) 54184 72794 35563 42641 29540 40883 31455 35880 21510 25264 22745 21683 TG (50:2) 30051 63840 16897 28274 14357 18499 7493 7341 4145 4586 6281 4409 TG (51:1) 3120 3137 4218 2343 3360 2599 2366 TG (52:0) 2560 2550 2448 2552 2918 2316 5210 5379 4850 3267 5001 TG (52:1) 13164 18700 12021 12542 11457 13290 26028 75359 26665 66540 26285 26337 TG (52:2) 31155 30728 18820 20591 17905 23263 31905 63177 25451 47929 25294 25341 TG (52:3) 95931 134277 54160 66345 45220 50323 34822 29675 17537 19326 21457 18428 TG (52:4) 84559 146807 45329 66112 36856 46493 11460 15336 7514 9121 10247 6968 TG (52:4) 62906 89454 33946 45368 31927 26805 27526 18125 13095 12606 18380 10506 TG (54:1) 9779 6017 4361 2840 2331 2998 2084 1892 2234 TG (54:2) 16251 48621 9019 18081 2465 12752 5129 5344 5519 6331 7431 5512 TG (54:3) 8447 8520 5608 5897 6215 10029 12914 30007 9893 25641 9664 9533 TG (54:4) 19662 23590 11180 14170 10469 11875 11482 16503 6724 12737 7454 6651 TG (54:6) 39310 87023 21329 37379 16984 22521 12637 24853 13660 20620 14751 12872 TG (56:1) 4408 5496 2755 4473 3052 2597 4153 3040 4059 2809 2723 3236 TG (56:2) 14848 41263 4727 17845 4277 12624 7387 6703 7965 8722 10308 7453 TG (56:4) 3151 9837 4917 5515 2415 3560 1684 TG (56:5) 3330 3734 2311 2248 2941 3183 9215 4746 8924 4151 4285 TG (56:6) 3952 3600 2779 2993 2770 2394 3345 5586 2081 4695 2124 2167 TG (58:1) 9629 8239 5378 6603 4329 3723 18406 35696 19410 31613 17016 17994 TG (58:2) 16362 17918 11504 15281 9754 8721 19553 32498 20968 28410 20723 19294 TG (58:4) 4046 7907 3471 6002 3020 3556 4509 6565 4935 5415 5208 4723 TG (58:6) 2814 4031 2344 2460 2735 2094 12558 4977 13118 4353 4899 TG (58:8) 3188 3547 2602 2409 2512 2673 5304 3575 5004 2942 3072 TG (60:2) 3619 4158 2673 3429 2447 7857 21665 8392 18827 7388 8324 CSH_posESI #015 4544 6635 3381 5207 2448 2474 4801 8930 4902 7322 5621 4900 CSH_posESI #036 2708 6303 3883 3178 4107 3878 3417 4959 3328 CSH_posESI #042 3184 5075 2035 4356 2010 2021 CSH_posESI #109 3473 3351 2772 17319 3975 2726 4118 2348 4048 1624 7868 3496 CSH_posESI #116 40253 48348 36191 34017 35699 38003 158634 76021 138065 64304 107337 137638 CSH_posESI #130 33480 39287 30775 30126 32338 32324 23837 10524 24290 9732 19704 22571 CSH_posESI #141 4922 6388 3586 4870 3491 3317 9065 19631 14720 17592 15107 9732 CSH_posESI #156 8154 12957 7470 8660 8211 21821 18181 24189 14419 23940 21476 CSH_posESI #196 3793 3276 2739 2599 3533 4303 2831 3032 2440 2660 3010 CSH_posESI #201 17381 14051 10816 180237 22886 7856 35678 21435 27975 13321 76961 19122 CSH_posESI #230 1518614 1803458 1399877 1343903 1430779 1524394 1435344 682962 1326074 576889 1063437 1272910 CSH_posESI #270 11614 9578 7296 7980 6788 6967 4303 1709 2209 1159 2091 2223 CSH_posESI #275 4114 8534 16823 7862 15420 8382 8545 CSH_posESI #285 25436 30019 20522 20181 26096 25309 10560 5594 10724 4726 7848 9223 CSH_posESI #288 290220 346161 282145 263796 298895 313030 445027 206909 428961 185685 358536 402867 CSH_posESI #301 237671 277155 228571 211331 241763 250534 361017 163734 337700 145219 287165 319740 CSH_posESI #307 19083 22083 16121 15852 19263 21163 12007 5381 10982 4676 9287 10499 CSH_posESI #308 7842 9025 6740 6176 5369 7395 14863 8058 14233 6369 11137 12934 CSH_posESI #363 34675 41106 31413 30571 35756 36544 42861 16791 40976 16588 36030 39883 0.56_131.16 45422 53044 39412 39786 43155 44150 38753 17509 37444 16222 31383 36064 0.56_130.26 4993 6084 4216 3810 5617 6311 17574 8549 15385 6777 11538 14576 0.66_167.99 64279 87458 58317 55950 63274 66500 113160 62178 108901 50639 83886 99405 0.72_279.09 28405 35895 18037 13929 24580 23382 14466 12958 5716 12945 19948 1.15_493.35 94297 115920 53686 51434 70785 67869 38654 45330 39510 17747 43341 66737 1.16_317.20 12840 2517 18104 6126 13596 11246 9739 1.16_155.07 21028 3897 4127 31451 10808 25793 19398 17824 1.16_473.25 666784 1305812 1060278 982181 1594215 806964 776701 390891 1301119 368430 848035 570242 1.16_457.28_1.16_435.30_1.16_452.32 90098 147289 128942 121167 169724 101730 95851 46502 136212 44762 97488 76787 1.16_479.33 136366 147997 143786 108113 137909 181923 100326 63684 51767 25312 79879 44357 1.18_412.32 168315 288406 251006 212139 290115 180274 188859 87119 243630 82280 185042 147236 1.37_707.16 1499054 2849309 2387363 2082315 3017506 1707221 1763493 818041 2461848 784041 1838331 1360035 1.37_709.16 36082 75471 35254 9646 18571 28743 31740 14849 21355 10446 15248 28884 1.37_156.14 1172976 1863913 1772126 1126014 1457057 1411072 1208197 593361 1391771 456796 1090521 988517 1.37_323.12_1.37_345.11 235616 377864 368873 223569 296149 281975 246245 117221 280384 91777 218184 197284 1.37_426.07 729634 1799884 700839 412414 575010 735670 801749 319393 670429 208981 548338 701962 1.40_353.23 4704 10112 24046 12209 23505 9021 21564 19436 1.42_300.99 781903 1949840 770069 470067 689430 872843 912942 347323 779280 233558 654547 786919 1.42_403.94 215584 316760 230149 151491 200808 134698 150542 118924 169843 57598 181039 143656 1.50_353.27 17927 22401 11708 10612 16633 18979 51217 27070 45471 20002 33005 40279 1.54_272.26 36669 47512 32352 31912 35913 35917 42720 21645 38103 18295 31334 37001 1.55_744.49 21497 26771 19233 19365 20962 22089 43196 20786 37285 17095 30249 36855 1.55_722.98 29451 17359 31408 13107 24918 27409 1.65_444.05 4975 6293 4259 4650 4762 5446 90570 45901 85438 36981 67321 76973 1.66_854.58 244324 329109 243707 137941 192745 144523 142310 101931 163427 48951 162268 130427 1.67_810.56 42709 48037 46257 36067 43438 39181 15346 7673 17946 6693 16929 15383 1.68_766.53 37392 48586 35883 33909 43534 41916 169027 84312 154475 71428 126091 152470 1.68_442.05 6682 9026 3236 3411 6398 7393 24212 12887 21959 9571 15350 19522 1.69_722.50 6334 7665 4725 5622 6332 29592 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5543 11.07_911.82 6884 5429 5302 5260 4870 6132 3896 2142 2566 1631 2340 2742 11.11_884.80 9777 7252 5788 5321 6130 5684 5469 3905 2788 3387 2917 2552 11.19_962.85 14496 9813 8119 7551 7597 7419 6643 4319 3356 3742 2796 2957 11.21_746.68 14645 8871 7250 7461 9233 8026 3058 5033 2449 7235 2726 11.21_1574.43 5023 7604 4925 7045 5561 4853 11.25_954.85 2747 4785 3102 4291 3739 3092 11.26_988.87 2531 3942 4819 9266 6071 7932 6378 5278 11.34_910.82 3744 7916 4639 6878 4191 4233 11.35_936.84 2468 7717 2834 7424 2101 2806 11.37_886.82 7245 8070 4839 6344 7093 8766 5601 3088 6351 2823 6297 5322 11.37_884.79 5618 6384 3869 5458 3149 2969 7784 19502 8653 16509 8201 8190 11.39_1514.46 2684 5905 3048 4956 2970 2825 11.41_912.84 5284 5146 3830 3749 3475 2975 4836 10272 5348 9029 4759 4753 11.42_939.86 2019 3930 3387 3631 2077 1973 11.46_938.85 2173 2548 4238 10767 4894 9657 4556 4702 11.53_722.68 6109 4837 3972 4031 3876 3942 5036 5983 4387 5102 3268 3932 11.53_836.81 9096 9224 4720 3368 7721 6141 6730 3819 8320 2068 6396 5280 11.53_990.88 2420 3412 6671 20002 8271 18070 8261 7240 11.54_367.34 2272 5535 2649 4996 2654 2172 11.54_862.82 2625 7051 3183 6818 3505 2694 11.56_957.86 3480 2923 2419 2494 2573 2954 9000 2722 10297 2189 2978 11.56_866.82 3266 7868 3484 6738 3652 3127 11.58_1588.48 2135 5027 2526 4544 2432 2383 11.65_982.88 2683 2712 2465 2409 2255 9641 2461 9695 2064 2474 11.72_914.85 2244 5378 2546 4815 2857 2347 11.74_1663.50 2899 3368 2294 2737 5988 15536 10381 13315 10315 5908 11.75_940.87 2480 4613 2704 4004 2546 2764 11.83_864.84 8713 9001 4462 3434 7558 6470 7186 4068 7882 2210 6246 5477 11.92_942.88 1912 5186 2204 4603 2038 2147 11.97_838.81 2321 2967 9698 32712 10864 30182 10581 9948 11.98_892.87 2602 5069 16003 5565 15026 5283 5138 12.00_1038.94 3319 11959 6894 10141 6793 6086 12.01_1012.93 2817 18661 7200 16879 5665 6521 12.04_970.91 2617 4264 3640 7020 4089 6333 4357 4115 12.04_936.89 2753 2350 2202 6432 3092 6513 2166 2793 12.06_996.93 2828 5370 2285 5119 2424 1949 12.07_866.84 3100 6180 2341 5516 2233 2004 12.09_712.67 2727 7076 2665 6703 2278 2793 12.12_965.93 4293 5436 4339 3824 4283 4175 9605 5383 9688 3945 5067 12.15_738.69 2612 6570 2831 6757 2346 2290 12.16_1433.92 3259 3905 2998 2292 2480 17682 6113 17437 4554 5418 12.16_750.70 3413 3502 3496 2569 2908 2699 7500 2463 7153 2197 2416 12.16_1470.92 2356 3366 2401 2180 2262 2476 4554 2520 5214 2342 2663 12.16_1263.19 3432 3410 3368 2268 2620 2489 8299 2056 7551 1827 2428 12.16_1470.43 7440 7086 4767 4454 9609 7218 980 8283 6580 5502 12.16_1264.19 3318 3326 3343 2512 7368 2012 7073 1751 2272 12.17_1544.94 7726 8456 6151 4518 9558 8491 952 8763 7286 6099 12.18_1581.95 18402 8268 17318 8251 17140 14759 15846 4865 19263 4299 6192 10052 12.18_1581.45 8939 8215 5589 4878 9545 8404 7489 1030 8761 809 8313 6137 12.18_1618.46 18258 6900 17487 8454 16927 15493 4498 19802 4295 6304 10121 12.19_1618.96 8116 8679 6500 5433 10534 9206 1250 8833 867 7909 6624 12.19_1655.97 9337 9011 5913 5144 10419 9797 1262 9830 791 7623 6288 12.19_1655.47 8043 9437 5862 4965 9938 9091 9131 1107 8938 859 7637 6107 12.19_1693.48 8660 8980 6777 5342 11444 8609 1223 9791 7626 6417 12.22_1331.25 8559 8372 6835 5678 11015 10117 1239 9445 913 7915 6555 12.22_1332.26 8781 9417 6625 5419 11437 10752 9110 1182 10626 882 8049 6726 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name retention index quantified m/z PubChem ID KEGG ID CE (18:1) 11.0525 673.5897_668.6326 CE (20:4) 10.3465 695.5740_690.6186 CE (22:6) 10.101 719.574 Ceramide (d18:1/23:0) 8.144 658.6101_636.6285 Ceramide (d40:1) 7.812333333 644.5951_622.6129_604.6019 Ceramide (d42:1) 8.458 672.6255_632.6330_650.6441 Ceramide (d42:2) 7.779666667 648.6266_670.6107_630.6179 Cholesterol 4.971 369.3517 Gal-Gal-Cer(d18:1/16:0) or Lactosylceramide(d18:1/16:0) 5.004 884.6153 GlcCer (d40:1) 7.146666667 806.6475_784.6647_766.6548 GlcCer (d42:1) 7.791666667 812.6968_834.6788_794.6861 GlcCer (d42:2) 7.121 832.6628_810.6787 LPC (16:0) 1.6 496.3399 LPC (18:0) 2.371 524.3712 LPC (18:1) 1.748 522.3556 PC (28:0) 4.338 678.5063 PC (30:0) 4.933 706.5420_728.5221 PC (30:1) 4.48 704.5224 PC (31:0) 5.255 720.5549 PC (32:0) 5.5815 734.5702_756.5521 PC (32:1) 5.003 732.5539 PC (33:0) 5.975 748.5903 PC (33:1) 5.328 746.5687 PC (34:0) 6.2485 762.6008_784.5827 PC (34:1) 5.64 760.5848 PC (34:2) 5.156 758.5686 PC (34:3) 4.779 756.5531 PC (34:4) 4.456 754.5376 PC (35:1) 5.965 774.5971 PC (36:1) 6.311 788.6166_810.6040 PC (36:2) 5.768 786.6007 PC (36:3) 5.298 784.5837 PC (36:4) 5.0535 782.5699_804.5524 PC (36:5) 4.584 780.5532 PC (38:2) 6.38 814.6351 PC (38:3) 5.964 812.6133 PC (38:4) 5.706 832.5824_810.601 PC (38:5) 5.108 808.5839_830.5664 PC (38:6) 4.913 806.5681_828.5525 PC (40:4) 6.118 838.6243 PC (40:5) 5.7305 836.6143_858.5964 PC (40:6) 5.515 834.5972 PC (40:7) 4.9545 832.5832_854.5686 PC (40:8) 4.537 830.5691 PC (42:5) 6.311 864.6465 PC (42:6) 5.691 862.6262 PC (42:7) 5.336 860.6059 PC (o-32:0) 5.9955 720.5897_742.5714 PC (o-34:0) 6.689 748.6209_770.6027 PC (p-34:0) or PC (o-34:1) 6.046 746.605 PC (p-34:1) or PC (o-34:2) 5.534 744.5893 PC (p-36:1) or PC (o-36:2) 6.142 772.6199 PC (p-36:3) or PC (o-36:4) 5.439 768.5856_790.5633 PC (p-36:4) or PC (o-36:5) 5.326 766.5707 PC (p-38:3) or PC (o-38:4) 6.114 796.6165 PC (p-38:4) or PC (o-38:5) 5.483 794.6005_816.5845 PC (p-38:5) or PC (o-38:6) 5.268 792.5881 PC (p-40:3) or PC (o-40:4) 6.546 824.6514 PC (p-40:4) or PC (o-40:5) 5.972 822.6360_844.6198 PC (p-40:6) or PC (o-40:7) 5.319 840.5828_818.6003 PC (p-42:3) or PC (o-42:4) 7.459 852.6833 PC (p-42:4) or PC (o-42:5) 6.506 850.6669 PC (p-42:5) or PC (o-42:6) 6.111 848.6503 PE (38:6) 5.014 764.5276 PE (p-36:4) or PE (o-36:5) 5.51 724.5278 PE (p-38:4) or PE (o-38:5) 6.179 752.5587 PE (p-38:5) or PE (o-38:6) 5.55 750.5438 PE (p-40:5) or PE (o-40:6) 6.195 778.5739 SM (d32:1) 4.245 675.5429 SM (d33:1) 4.543 689.5588 SM (d34:1) 4.8515 703.5747_725.5569 SM (d34:2) 4.341 701.5585 SM (d36:1) 5.516 731.6056_753.5753 SM (d38:1) 6.202 759.6366_781.6133 SM (d39:1) 6.571 773.6494 SM (d40:1) 6.9045 787.6685_809.6502 SM (d41:1) 7.253 801.684 SM (d41:2) 6.559 799.6665 SM (d42:1) 7.5885 815.6998_837.6815 SM (d42:2) 6.8805 813.6843_835.6660 SM (d42:3) 6.3075 811.6663_833.6497 SM (d43:2) 7.216 827.6982 TG (14:0/14:0/14:0) 9.617 740.675 TG (46:0) 10.576 796.7383 TG (48:0) 10.9985 829.7256_824.7695 TG (48:1) 10.597 822.7543 TG (48:2) 10.183 820.7382 TG (49:0) 11.174 838.7849 TG (50:0) 11.3865 857.7569_852.8010 TG (50:1) 11.002 855.7410_850.7854 TG (50:2) 10.623 848.7699 TG (51:1) 11.183 864.7997 TG (52:0) 11.7225 885.789_880.8323 TG (52:1) 11.3835 883.7729_878.8168 TG (52:2) 11.0165 881.7566_876.8011 TG (52:3) 10.6625 874.7856_879.7439 TG (52:4) 10.4195 877.7277_872.7693 TG (52:4) 10.4565 903.743_898.7849 TG (54:1) 11.732 906.8478 TG (54:2) 11.392 904.8321 TG (54:3) 11.0435 902.8154_907.7781 TG (54:4) 10.795 900.8012 TG (54:6) 9.972 896.7691 TG (56:1) 11.922 934.8777 TG (56:2) 11.731 932.8638 TG (56:4) 11.22 933.7858_928.8321 TG (56:5) 10.931 931.7716_926.8162 TG (56:6) 10.745 924.7975 TG (58:1) 11.996 962.9089 TG (58:2) 11.921 960.8913 TG (58:4) 11.566 956.8642 TG (58:6) 10.911 952.8322 TG (58:8) 10.403 948.8003 TG (60:2) 12.039 988.9272 CSH_posESI #015 3.485 279.1603 CSH_posESI #036 4.9835 858.5982_880.5814 CSH_posESI #042 5.707 900.5699 CSH_posESI #109 11.177 929.8334 CSH_posESI #116 9.5765 885.6728_880.7174 CSH_posESI #130 6.876 903.6534 CSH_posESI #141 11.389 899.7473 CSH_posESI #156 3.481 803.5432 CSH_posESI #196 11.009 897.7305 CSH_posESI #201 10.778 369.3517 CSH_posESI #230 5.156 848.5396 CSH_posESI #270 4.981 627.5351 CSH_posESI #275 4.981666667 909.5463_904.5908_887.5644 CSH_posESI #285 5.785 876.5694 CSH_posESI #288 5.008 577.5185 CSH_posESI #301 5.644 850.5549 CSH_posESI #307 5.054 872.5389 CSH_posESI #308 6.307 878.5854 CSH_posESI #363 5.672 792.5608 0.56_131.16 0.56 131.1623 0.56_130.26 0.561 130.2645 0.66_167.99 0.664 167.9935 0.72_279.09 0.721 279.0937 1.15_493.35 1.155 493.3483 1.16_317.20 1.156 317.1958 1.16_155.07 1.156 155.0703 1.16_473.25 1.156 473.2513 1.16_457.28_1.16_435.30_1.16_452.32 1.158 457.2775_435.2953_452.3221 1.16_479.33 1.16 479.3325 1.18_412.32 1.184 412.3212 1.37_707.16 1.367 707.1637 1.37_709.16 1.367 709.1619 1.37_156.14 1.369 156.1381 1.37_323.12_1.37_345.11 1.3695 323.1249_345.1065 1.37_426.07 1.37 426.073 1.40_353.23 1.403 353.2302 1.42_300.99 1.416 300.9924 1.42_403.94 1.416 403.9406 1.50_353.27 1.496 353.2662 1.54_272.26 1.54 272.2585 1.55_744.49 1.547 744.4931 1.55_722.98 1.552 722.9807 1.65_444.05 1.646 444.0481 1.66_854.58 1.659 854.5823 1.67_810.56 1.669 810.5565 1.68_766.53 1.68 766.5301 1.68_442.05 1.683 442.0499 1.69_722.50 1.687 722.5038 1.70_678.48 1.699 678.4782 1.71_634.45 1.708 634.4521 1.72_546.40 1.716 546.3998 1.72_590.43 1.717 590.426 1.74_502.37_1.74_507.33 1.737 502.3736_507.3290 1.75_419.28_1.75_414.32_1.75_397.29 1.745333333 419.2763_414.3213_397.2949 1.75_370.29_1.75_375.25 1.746 370.2947_375.2502 1.74_463.30_1.75_441.32_1.75_458.35 1.746 463.3026_441.3198_458.3473 1.78_553.33 1.78 553.3349 1.90_156.14 1.896 156.1381 1.90_667.22 1.897 667.2238 1.90_323.12_1.90_345.11 1.898 323.1249_345.1067 1.94_604.44 1.941 604.4415 1.95_560.42 1.955 560.4153 1.96_516.39 1.965 516.3893 2.00_482.38 2 482.381 2.03_833.24 2.03 833.2353 2.03_835.23 2.03 835.2344 2.05_786.18 2.046 786.1764 2.05_788.18 2.048 788.1758 2.06_739.12 2.064 739.1178 2.06_741.12 2.064 741.116 2.07_403.02 2.066 403.0217 2.07_401.02 2.067 401.0237 2.29_300.29_2.29_322.27 2.2885 300.2901_322.2719 2.29_599.57_2.29_621.55 2.29 599.5719_621.5542 2.45_433.00 2.454 432.996 2.46_434.99 2.455 434.9935 2.46_371.07 2.455 371.074 2.54_361.23 2.542 361.2342 2.56_297.28 2.563 297.2791 2.56_337.27 2.564 337.2713 2.62_330.30_2.62_313.27_2.62_335.26 2.62 330.303_313.2738_335.2558 2.83_397.38 2.834 397.379 2.89_439.13 2.894 439.1333 2.90_441.13 2.895 441.1311 2.90_480.16 2.896 480.1595 2.90_817.33 2.897 817.3346 2.90_815.34 2.898 815.3363 3.03_455.13 3.031 455.128 3.03_395.20 3.031 395.2041 3.03_393.21_3.03_415.19 3.0315 393.2066_415.1886 3.04_326.31 3.041 326.3056 3.06_372.36 3.061 372.3574 3.08_655.63_3.07_677.62 3.0745 655.6347_677.6167 3.07_328.32_3.08_350.30 3.0745 328.3213_350.3034 3.08_328.49 3.075 328.4853 3.11_487.24 3.114 487.2423 3.17_421.24_3.16_443.22 3.166 421.2355_443.2170 3.19_284.30 3.187 284.2951 3.21_670.31 3.209 670.3084 3.21_879.28 3.213 879.2808 3.21_881.28 3.214 881.2789 3.23_409.18_3.22_431.17 3.221 409.1839_431.1656 3.23_411.18_3.22_433.16 3.221 411.1810_433.1627 3.22_839.34 3.223 839.3422 3.22_174.11 3.225 174.1123 3.22_280.03 3.225 280.0317 3.23_172.12 3.225 172.1154 3.23_413.18 3.225 413.1782 3.23_612.26 3.226 612.264 3.23_204.09 3.226 204.0876 3.23_116.05 3.227 116.0529 3.23_616.26 3.228 616.2588 3.23_447.14 3.23 447.1395 3.24_467.24 3.237 467.2367 3.25_471.11 3.248 471.1054 3.25_473.10 3.25 473.1032 3.30_370.33 3.299 370.3314 3.31_310.31 3.311 310.3106 3.37_572.45 3.366 572.4511 3.40_378.30 3.402 378.2983 3.49_149.02 3.486 149.0234 3.49_391.28 3.487 391.2847 3.49_167.04 3.487 167.036 3.49_113.13 3.49 113.134 3.50_783.57 3.498 783.5741 3.50_71.09 3.499 71.0884 3.52_111.04 3.515 111.0446 3.52_393.30_3.52_388.34 3.5165 393.2978_388.3423 3.52_147.07 3.517 147.0652 3.52_409.27 3.517 409.2715 3.52_259.19 3.517 259.1905 3.52_371.32 3.518 371.316 3.52_241.18 3.518 241.1799 3.52_129.05 3.519 129.0547 3.52_101.06 3.519 101.0602 3.52_763.61 3.521 763.606 3.53_430.39 3.526 430.3877 3.54_354.34 3.539 354.3368 3.58_515.27 3.579 515.2738 3.59_356.35 3.586 356.3523 3.59_588.45 3.594 588.4465 3.60_841.56 3.599 841.555 3.61_487.22 3.615 487.2216 3.62_507.32 3.615 507.3188 3.62_919.51 3.62 919.5052 3.63_1013.60 3.626 1013.5998 3.64_449.27_3.62_471.25 3.628 449.2661_471.2476 3.63_452.27 3.635 452.2664 3.63_282.28 3.635 282.2793 3.64_1091.55 3.637 1091.5508 3.67_793.51 3.668 793.5064 3.69_867.52_3.69_884.54 3.687 867.5161_884.5431 3.72_312.33 3.719 312.3264 3.73_988.66 3.729 988.6563 3.73_385.27 3.729 385.2741 3.74_843.52_3.76_865.50 3.748 843.5182_865.4985 3.78_433.29 3.777 433.2886 3.79_267.02 3.788 267.0172 3.79_532.17 3.79 532.1652 3.79_744.12 3.79 744.1163 3.79_308.04 3.791 308.0439 3.79_310.04 3.791 310.0418 3.79_530.17 3.791 530.1659 3.79_516.15 3.791 516.15 3.79_738.12 3.792 738.1226 3.79_860.55 3.793 860.5482 3.79_740.12 3.793 740.1208 3.79_472.11 3.794 472.1119 3.80_475.11 3.796 475.1073 3.80_733.13 3.797 733.1279 3.80_473.11 3.799 473.1057 3.80_471.11 3.799 471.1054 3.80_735.12 3.8 735.1239 3.80_600.48 3.802 600.4834 3.85_679.55 3.845 679.5477 3.88_361.27 3.876 361.2741 3.90_419.32 3.901 419.3158 3.90_859.61 3.905 859.6052 3.92_463.35 3.921 463.3525 3.93_886.56 3.93 886.5589 3.95_387.29 3.945 387.2894 3.95_477.37 3.953 477.3692 4.05_862.56 4.054 862.5589 4.09_871.55_4.09_888.57 4.0935 871.5472_888.5741 4.10_366.37 4.103 366.3732 4.18_455.42 4.179 455.4203 4.18_480.30 4.182 480.2974 4.18_310.31 4.182 310.3107 4.18_515.25 4.182 515.2528 4.18_477.30_4.18_499.28 4.1835 477.2975_499.2791 4.18_975.57 4.184 975.5683 4.19_535.35 4.187 535.3502 4.29_562.44 4.291 562.4438 4.33_487.38 4.334 487.3761 4.39_449.36_4.39_444.40_4.39_427.38 4.387666667 449.3601_444.4045_427.3782 4.39_471.42 4.388 471.4153 4.39_465.33 4.389 465.3343 4.39_485.43 4.391 485.431 4.40_854.57 4.404 854.5691 4.41_599.50 4.407 599.503 4.46_625.52 4.46 625.5183 4.47_885.55 4.467 885.548 4.60_856.58 4.6 856.5844 4.62_692.52 4.621 692.5227 4.66_807.57 4.658 807.5721 4.69_852.25 4.687 852.2506 4.74_620.50 4.736 620.5012 4.74_626.58 4.737 626.5825 4.74_582.55_4.74_604.53 4.7375 582.5455_604.5276 4.75_769.56 4.753 769.5568 4.78_505.43_4.79_483.45 4.7885 505.4336_483.4519 4.79_718.54 4.789 718.5383 4.80_740.56 4.804 740.5567 4.82_882.60 4.819 882.6 4.83_741.54 4.835 741.538 4.85_793.54 4.846 793.5447 4.85_1077.34 4.851 1077.3419 4.85_1406.14_4.85_1428.12 4.852 1406.1414_1428.1229 4.85_184.07 4.852 184.0732 4.85_705.81 4.853 705.8149 4.88_762.54 4.883 762.5392 4.89_790.56 4.887 790.5635 4.89_793.58 4.893 793.5791 4.90_730.55 4.897 730.5452 4.93_783.57 4.931 783.5697 4.93_836.54 4.934 836.5442 4.94_767.57 4.938 767.5747 4.94_778.57 4.94 778.5688 4.94_729.55 4.944 729.5517 4.94_729.58 4.944 729.5765 4.95_661.53 4.95 661.527 4.95_515.41 4.952 515.4061 4.99_796.52 4.989 796.5244 4.99_736.52 4.989 736.5159 4.99_752.55 4.994 752.5501 5.00_913.58 4.998 913.5796 5.00_653.55 4.999 653.5491 5.00_735.56 4.999 735.5612 5.02_762.53 5.023 762.5289 5.04_727.57 5.043 727.5698 5.05_722.52 5.052 722.5238 5.06_820.53 5.058 820.529 5.06_754.54 5.063 754.5423 5.07_860.56 5.074 860.5613 5.08_840.60 5.077 840.6024 5.08_818.06 5.081 818.0604 5.09_817.57 5.087 817.5677 5.10_812.55 5.098 812.5468 5.11_839.56 5.107 839.561 5.11_898.55 5.108 898.554 5.11_723.55 5.11 723.5545 5.11_846.55 5.111 846.5451 5.11_838.56 5.113 838.5573 5.11_700.57_5.14_722.55 5.122 700.5715_722.5509 5.13_776.56 5.126 776.5559 5.14_535.28 5.136 535.2845 5.14_791.57 5.139 791.5708 5.14_802.30 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