#METABOLOMICS WORKBENCH araskind_20160413_131722 DATATRACK_ID:586 STUDY_ID:ST000375 ANALYSIS_ID:AN000609 PROJECT_ID:PR000295 VERSION 1 CREATED_ON April 19, 2016, 3:02 pm #PROJECT PR:PROJECT_TITLE Metabolomics Mice Studies PR:PROJECT_SUMMARY Analysis of glycolysis/TCA cycle in skeletal muscle + acyl-carnitine in serum of PR:PROJECT_SUMMARY wt and 113Q mice PR:INSTITUTE University of Michigan PR:DEPARTMENT Pathology PR:LABORATORY Lieberman, Andrew PR:LAST_NAME Lieberman PR:FIRST_NAME Andrew PR:ADDRESS Ann Arbor, MI PR:EMAIL liebermn@umich.edu PR:PHONE 734-936-1889 #STUDY ST:STUDY_TITLE Mice exercise metabolomics (part II) ST:STUDY_TYPE Regular ST:STUDY_SUMMARY Mice were run for 15 minutes on a treadmill and then mice were induced into ST:STUDY_SUMMARY anesthesia at a dose of 5% isoflurane, then maintained by continuous inhalation ST:STUDY_SUMMARY of 2% isoflurane. Quadriceps muscle was collected first and flash frozen in ST:STUDY_SUMMARY liquid nitrogen. Then blood was collected from the left ventricle of the heart ST:STUDY_SUMMARY and placed into serum separating tubes. Blood was allowed to clot for 40 minutes ST:STUDY_SUMMARY and centrifuged, the supernatant was frozen in liquid nitrogen ST:INSTITUTE University of Michigan ST:DEPARTMENT Biomedical Research Core Facilities ST:LABORATORY Metabolomics core ST:LAST_NAME Kachman ST:FIRST_NAME Maureen ST:ADDRESS 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 ST:EMAIL mkachman@med.umich.edu ST:PHONE (734) 232-8175 ST:NUM_GROUPS 4 ST:TOTAL_SUBJECTS 18 #SUBJECT SU:SUBJECT_TYPE Animal SU:SUBJECT_SPECIES Mus musculus SU:TAXONOMY_ID 10090 #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS SU0012359 S00018461 Wild Type:yes | Mutants:no SUBJECT_SAMPLE_FACTORS SU0012360 S00018462 Wild Type:yes | Mutants:no SUBJECT_SAMPLE_FACTORS SU0012361 S00018463 Wild Type:yes | Mutants:no SUBJECT_SAMPLE_FACTORS SU0012362 S00018464 Wild Type:yes | Mutants:no SUBJECT_SAMPLE_FACTORS SU0012363 S00018465 Wild Type:no | Mutants:yes SUBJECT_SAMPLE_FACTORS SU0012364 S00018466 Wild Type:no | Mutants:yes SUBJECT_SAMPLE_FACTORS SU0012365 S00018467 Wild Type:no | Mutants:yes SUBJECT_SAMPLE_FACTORS SU0012366 S00018468 Wild Type:no | Mutants:yes SUBJECT_SAMPLE_FACTORS SU0012359 S00018469 Wild Type:yes | Mutants:no SUBJECT_SAMPLE_FACTORS SU0012360 S00018470 Wild Type:yes | Mutants:no SUBJECT_SAMPLE_FACTORS SU0012361 S00018471 Wild Type:yes | Mutants:no SUBJECT_SAMPLE_FACTORS SU0012362 S00018472 Wild Type:yes | Mutants:no SUBJECT_SAMPLE_FACTORS SU0012363 S00018473 Wild Type:no | Mutants:yes SUBJECT_SAMPLE_FACTORS SU0012364 S00018474 Wild Type:no | Mutants:yes SUBJECT_SAMPLE_FACTORS SU0012365 S00018475 Wild Type:no | Mutants:yes SUBJECT_SAMPLE_FACTORS SU0012366 S00018476 Wild Type:no | Mutants:yes #COLLECTION CO:COLLECTION_SUMMARY NONE CO:SAMPLE_TYPE Serum #TREATMENT TR:TREATMENT_SUMMARY None #SAMPLEPREP SP:SAMPLEPREP_SUMMARY none SP:SAMPLEPREP_PROTOCOL_FILENAME Gly-TCA-nucleotides analysis protocol-2015-03-09.docx #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE None CH:INSTRUMENT_NAME QTOF-002-HILIC-35min-1mm-No Insert.m.zip CH:COLUMN_NAME Phenomenex Luna NH2 (150 x 1mm, 3um) CH:METHODS_ID DQM0166 #ANALYSIS AN:ANALYSIS_TYPE MS AN:ANALYSIS_PROTOCOL_FILE QTOF-002-HILIC-35min-1mm-No Insert.m.zip #MS MS:MS_COMMENTS - MS:INSTRUMENT_NAME Agilent 6410A QQQ MS:INSTRUMENT_TYPE Triple quadrupole MS:MS_TYPE ESI MS:ION_MODE NEGATIVE #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS pmol/mg tissue MS_METABOLITE_DATA_START Samples S00018469 S00018470 S00018471 S00018472 S00018473 S00018474 S00018475 S00018476 Factors Wild Type:yes | Mutants:no Wild Type:yes | Mutants:no Wild Type:yes | Mutants:no Wild Type:yes | Mutants:no Wild Type:no | Mutants:yes Wild Type:no | Mutants:yes Wild Type:no | Mutants:yes Wild Type:no | Mutants:yes 2 or 3-phosphoglycerate 2.287 0.601 1.617 1.770 1.250 0.889 1.819 0.801 6-phosphogluconate 7.399 7.198 8.152 7.461 7.076 7.302 7.475 7.130 acetyl-coa adenosine diphosphate 95.155 63.713 71.123 60.065 51.669 66.962 80.071 70.488 adenosine monophosphate 52.512 4.211 21.349 31.086 25.919 27.713 44.820 31.979 adenosine triphosphate 2.158 2.308 3.137 3.259 2.036 2.810 3.619 2.114 citrate or isocitrate 221.192 235.857 137.673 100.820 183.330 162.433 126.434 116.209 erythrose 4-phosphate 7.695 19.532 17.975 18.527 27.396 11.619 13.400 14.021 glucose 6-phosphate or fructose-6-phosphate 6.693 9.202 7.663 7.470 8.534 6.363 6.848 6.954 flavin adenine dinucleotide 2.338 3.334 1.699 2.482 2.492 1.751 1.830 1.754 fructose 1,6-bisphosphate 21.110 18.351 22.241 19.877 17.386 18.850 19.063 18.546 malate 63.219 99.217 38.470 67.778 97.975 37.941 51.511 38.968 nicotinamide adenine dinucleotide oxidized 25.118 8.027 18.917 16.636 6.957 12.091 9.170 9.217 nicotinamide adenine dinucleotide reduced 0.208 0.090 0.347 0.259 0.070 0.300 0.211 0.232 nicotinamide adenine dinucleotide phosphate oxidized 0.607 0.191 0.597 0.488 0.250 0.310 0.522 0.291 nicotinamide adenine dinucleotide phosphate reduced phosphoenolpyruvate ribose-5-phosphate or xylulose 5-phosphate 118.434 144.870 96.410 118.674 115.499 128.752 97.159 86.947 sedoheptulose 7-phosphate 1.680 1.788 1.009 1.315 2.725 1.746 2.269 1.275 sucrose MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name retention index quantified m/z PubChem ID KEGG ID 2 or 3-phosphoglycerate 724 6-phosphogluconate 91493 acetyl-coa 444493 adenosine diphosphate 6022 adenosine monophosphate 6083 adenosine triphosphate 5957 citrate or isocitrate 311 erythrose 4-phosphate 122357 glucose 6-phosphate or fructose-6-phosphate 5958 flavin adenine dinucleotide 46906035 fructose 1,6-bisphosphate 21125049 malate 525 nicotinamide adenine dinucleotide oxidized 15938971 nicotinamide adenine dinucleotide reduced 439153 nicotinamide adenine dinucleotide phosphate oxidized 15938972 nicotinamide adenine dinucleotide phosphate reduced 22833512 phosphoenolpyruvate 1005 ribose-5-phosphate or xylulose 5-phosphate 77982 sedoheptulose 7-phosphate 165007 sucrose 5988 METABOLITES_END #END