#METABOLOMICS WORKBENCH yli_20161229_150141 DATATRACK_ID:807 STUDY_ID:ST000531 ANALYSIS_ID:AN000810 PROJECT_ID:PR000390 VERSION 1 CREATED_ON January 17, 2017, 10:29 am #PROJECT PR:PROJECT_TITLE CHEAR Plasma Reference Material Proficiency Test PR:PROJECT_TYPE Cytokine Analysis PR:PROJECT_SUMMARY CHEAR Reference Material Plasma was provided by Emory University. The material PR:PROJECT_SUMMARY was prepared and analyzed using the cytokine array workflow of the Eastern PR:PROJECT_SUMMARY Regional Metabolomics Resource Core (protocols based on Ray Biotech's PR:PROJECT_SUMMARY manufacturer recommendations). Six replicate aliquots of the pooled reference PR:PROJECT_SUMMARY sample were analyzed on 6 arrays. PR:INSTITUTE RTI International PR:DEPARTMENT CHEAR - Children's Health Exposure Analysis Resource PR:LAST_NAME Fennell PR:FIRST_NAME Timothy PR:ADDRESS 3040 East Cornwallis Road, RTI International, Little Building, Research Triangle PR:ADDRESS Park, North Carolina, 27709, USA PR:EMAIL Fennell@rti.org PR:PHONE 919 485 2781 #STUDY ST:STUDY_TITLE CHEAR Pooled Plasma Reference Material Proficiency Test ST:STUDY_TYPE Cytokine Analysis ST:STUDY_SUMMARY CHEAR Reference Material Plasma was provided by Emory University. The material ST:STUDY_SUMMARY was prepared and analyzed using the cytokine array workflow of the Eastern ST:STUDY_SUMMARY Regional Metabolomics Resource Core (protocols based on Ray Biotech's ST:STUDY_SUMMARY manufacturer recommendations). Six replicate aliquots of the pooled reference ST:STUDY_SUMMARY sample were anlyzed on 6 arrays. ST:INSTITUTE RTI International ST:DEPARTMENT Analytical Chemistry & Pharmaceuticals ST:LABORATORY CHEAR CC - UARC ST:LAST_NAME Fennell ST:FIRST_NAME Timothy ST:ADDRESS 3040 E. Cornwallis Road, Research Triangle Park, NC 27709 ST:EMAIL fennell@rti.org ST:PHONE 919-485-2781 #SUBJECT SU:SUBJECT_TYPE Human SU:SUBJECT_SPECIES Homo sapiens SU:TAXONOMY_ID 9606 #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS Pooled Plasma 14402855_A1 SampleType:Replicate Subject identifier (if any)=CHE000054 0200 SUBJECT_SAMPLE_FACTORS Pooled Plasma 14402855_A2 SampleType:Replicate Subject identifier (if any)=CHE000054 0200 SUBJECT_SAMPLE_FACTORS Pooled Plasma 14402855_A3 SampleType:Replicate Subject identifier (if any)=CHE000054 0200 SUBJECT_SAMPLE_FACTORS Pooled Plasma 14402855_A4 SampleType:Replicate Subject identifier (if any)=CHE000054 0200 SUBJECT_SAMPLE_FACTORS Pooled Plasma 14402855_A5 SampleType:Replicate Subject identifier (if any)=CHE000054 0200 SUBJECT_SAMPLE_FACTORS Pooled Plasma 14402855_A6 SampleType:Replicate Subject identifier (if any)=CHE000054 0200 #COLLECTION CO:COLLECTION_SUMMARY Provided by CHEAR #TREATMENT TR:TREATMENT_SUMMARY N.A. #SAMPLEPREP SP:SAMPLEPREP_SUMMARY Pooled plasma was shipped to the NIH RTI-RCMRC on dry ice and immediately stored SP:SAMPLEPREP_SUMMARY at -80 °C after being logged in for metabolomics analysis. One aliquot was SP:SAMPLEPREP_SUMMARY thawed on ice prior to hybridization on Ray Biotech Cytokine Array G5 (Cat # SP:SAMPLEPREP_SUMMARY AAH-CYT-G5-8). Procedures were followed according to the manufacturer’s SP:SAMPLEPREP_SUMMARY recommendations. #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE fluorescent detection CH:INSTRUMENT_NAME Molecular Devices, GenePix Personal 4000 scanner CH:COLUMN_NAME N.A. #ANALYSIS AN:ANALYSIS_TYPE MS #MS MS:INSTRUMENT_NAME GenePix Personal 4000 scanner MS:INSTRUMENT_TYPE fluorescent MS:MS_TYPE Other MS:ION_MODE UNSPECIFIED #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS ABS MS_METABOLITE_DATA_START Samples 14402855_A1 14402855_A2 14402855_A3 14402855_A4 14402855_A5 14402855_A6 Factors SampleType:Replicate SampleType:Replicate SampleType:Replicate SampleType:Replicate SampleType:Replicate SampleType:Replicate Angiogenin 71175.8166 68822.08498 65360.62108 62294.86591 62511.81661 63088.76202 BDNF 689.9074689 573.0131162 852.3516104 828.6603826 958.1509692 731.5925957 BLC 932.0279923 1052.891869 1111.306528 1350.44481 970.1099179 1177.014737 Ck beta 8-1 210.5632417 189.4258235 183.4680592 187.7470907 189.9081052 201.2362219 EGF 2353.193851 2248.379289 2649.53873 2825.73667 2505.160572 2388.775296 ENA-78 565.3106153 708.2421069 1179.294691 1600.13891 1186.806068 666.9267594 Eotaxin 1802.029603 1397.541632 1407.255005 1446.700933 1632.157318 1294.764469 Eotaxin-2 2744.939417 1253.893715 2681.033247 2880.059432 2975.864793 2786.904512 Eotaxin-3 216.5482434 174.1665211 224.4609226 212.0493791 228.655099 185.7936341 FGF-4 372.1582876 297.8194892 401.4301133 337.3729447 350.1580177 278.9317416 FGF-6 393.9219302 298.3456721 434.9242821 311.1645945 485.5333169 285.6878738 FGF-7 304.1469047 268.35325 329.9425589 313.5471718 392.7318751 274.5885138 FGF-9 350.3946451 343.0712138 439.4234988 413.1389027 475.4878 290.5136825 Flt-3 Ligand 797.0934084 977.6477227 1269.279026 1161.744688 1024.164366 716.1500079 Fractalkine 144.728223 139.9646363 109.48094 113.4106792 173.1655771 87.82971796 G-CSF 593.6033506 564.0680079 807.3594433 825.8012899 847.1719254 807.8403728 GCP-2 198.5932383 101.5532887 90.98416017 73.38338063 227.2200252 63.70067456 GDNF 448.3310366 506.714078 553.9035685 472.703335 512.321362 422.7408403 GM-CSF 618.0874485 575.6440305 1019.822455 963.9907729 935.1897878 819.9048945 GRO 108.8182128 82.61070638 114.9799826 111.5046173 146.377532 81.0735858 GRO-alpha 114.8032145 79.45360932 122.9785901 114.8402255 133.9402254 145.2568412 HGF 313.9405438 382.534927 403.9296781 357.8631095 319.5431091 310.7820789 I-309 69.0995651 75.77032942 90.98416017 122.9409883 134.4185833 141.3961943 IFN-gamma 447.2428544 544.5992427 712.3759793 846.7679701 776.8533071 576.6841371 IGF-I 168.1241387 31.04478775 97.98294172 71.95383426 204.2588437 98.44649705 IGFBP-1 5495.863835 7897.478293 9845.285991 7886.807356 6668.309791 7347.776294 IGFBP-2 2225.332451 1906.360441 2681.53316 2389.24851 2376.482284 2347.273341 IGFBP-3 320.4696366 286.7696495 393.4315058 342.1380993 316.6729614 329.1201519 IGFBP-4 112.6268502 25.78295932 60.98938209 98.16218449 147.3342479 32.81549902 IL-1 alpha 432.0083046 385.6920241 677.8819846 627.5708591 706.0563308 516.8441095 IL-1 beta 117.5236698 136.2813564 252.9559618 79.10156614 242.0491215 247.0814044 IL-10 748.6693038 816.6357726 1205.790079 1098.844648 1058.606138 898.565576 IL-12 p70 347.6741898 317.8144373 502.4125328 416.9510263 503.7109189 390.890503 IL-13 661.0706425 717.7133981 957.8332466 929.6816599 923.709197 658.7228847 IL-15 533.7533336 613.5291952 809.3590951 935.8763608 734.7578077 608.5344744 IL-16 391.7455659 354.1210535 448.4219323 361.6752331 395.1236648 295.3394911 IL-2 582.7215293 557.7538138 1033.820018 829.6134135 898.3562258 783.2287486 IL-3 350.3946451 286.2434667 559.4026111 384.0714597 558.7220829 380.2737239 IL-4 504.9165072 343.0712138 747.8698001 447.4480157 810.3383634 395.7163117 IL-5 719.2883863 718.7657638 945.8353354 1085.025699 1066.738223 887.9487969 IL-6 681.2020119 793.4837275 869.8485643 933.970299 986.8524461 836.7952249 IL-7 543.5469727 683.5115133 812.3585729 1129.818152 1075.827024 895.1875099 IL-8 825.3861438 792.9575447 1159.798086 1125.052998 1133.229978 1122.483099 IP-10 1778.089596 1627.483534 1667.709661 1675.904868 2058.374249 1322.754159 LIF 365.0851038 273.6150785 461.4196694 240.1637912 453.4833344 198.3407367 LIGHT 290.000537 296.2409407 334.4417756 299.251708 304.7140127 262.0414113 Leptin 6616.691427 24083.91492 33252.71089 17024.94431 8004.363539 26111.96818 MCP-1 285.1037174 322.0239 351.9387294 383.1184288 340.5908588 383.1692091 MCP-2 139.8314034 161.01195 280.951088 183.934967 248.7461328 186.7587959 MCP-3 406.9801157 354.1210535 564.4017408 403.132078 563.5056624 341.6672545 MCP-4 138.1991302 68.92995246 94.48355094 67.18867967 105.2387485 87.34713709 MCSF 135.4786749 154.6977559 157.4725849 185.8410289 205.2155596 131.2619961 MDC 1059.345301 1510.14476 1552.729678 1655.414703 1459.470098 1239.75025 MIF 469.550588 434.6270285 442.4229766 335.4668829 421.4333519 246.5988235 MIG 835.1797829 894.5108334 908.8417758 922.0574125 924.187555 772.6119695 MIP-1 beta 1068.594849 1345.44953 1807.185379 1553.916911 1431.725337 1458.359383 MIP-1delta 4286.3494 6315.246484 7852.632901 8003.553644 7439.422803 6611.840471 MIP-3 alpha 220.3568808 270.9841642 200.4651002 197.2773999 269.3155246 160.699429 NAP-2 11112.51589 15369.27467 18177.83534 15406.22129 18806.64271 14215.38463 NEG 4.284717127 6.182648408 6.186422979 5.36079891 4.962963707 4.222582594 NT-3 516.3424195 369.3803559 549.4043518 421.7161809 532.4123958 281.8272268 NT-4 221.989154 262.0390559 219.4617929 212.5258946 205.6939175 189.6542811 Oncostatin M 218.1805166 162.0643157 177.4691036 182.0289052 253.0513543 133.1923195 Osteopontin 5902.29986 6173.703299 7675.66371 6985.240109 6149.291418 6228.671262 Osteoprotegerin 167.5800476 166.2737784 197.9655353 190.129668 225.7849513 150.5652308 PARC 529.4006051 425.1557373 539.9060054 441.7298302 445.8296073 362.4182318 PDGF-BB 25597.85228 34610.20269 39566.11176 38628.24914 32323.60324 42498.96671 PIGF 205.6664221 34.72806765 257.4551785 206.8077091 200.4319801 172.7639507 POS 43877.86111 43877.86111 43877.86111 43877.86111 43877.86111 43877.86111 RANTES 71194.3157 68845.23702 65386.61656 62291.5303 62567.30613 63110.96074 SCF 379.2314715 252.5677647 385.4328983 390.2661606 337.2423531 338.7717693 SDF-1 346.5860076 192.0567378 288.9496955 336.8964293 387.9482956 275.0710947 TARC 1160.546239 923.4508898 1724.699739 1221.309121 1528.832001 918.3513916 TGF-beta 1 784.579314 779.2767908 1244.78329 959.2256183 1129.403115 727.2493679 TGF-beta 2 248.6496161 245.2012049 321.4440384 218.7205955 360.2035346 204.614288 TGF-beta 3 118.0677608 152.5930245 173.4697999 152.9614622 197.0834745 173.7291124 TIMP-1 8358.871013 9345.533478 10331.2014 8888.919366 10065.60794 6817.902501 TIMP-2 4654.699051 5336.546396 5589.026982 4826.625081 4981.619667 2954.360073 TNF-alpha 801.4461369 413.0535319 1061.815144 993.5347313 1237.512011 856.0984596 TNF-beta 1216.043528 988.1713795 1595.722194 1062.152957 1651.769993 960.3359271 Thrombopoietin 146.3604962 166.7999613 194.4661445 216.8145337 191.821537 173.2465316 VEGF 62.02638127 39.46371324 39.99303744 42.88639128 65.05668089 38.12388856 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name Angiogenin BDNF BLC Ck beta 8-1 EGF ENA-78 Eotaxin Eotaxin-2 Eotaxin-3 FGF-4 FGF-6 FGF-7 FGF-9 Flt-3 Ligand Fractalkine G-CSF GCP-2 GDNF GM-CSF GRO GRO-alpha HGF I-309 IFN-gamma IGF-I IGFBP-1 IGFBP-2 IGFBP-3 IGFBP-4 IL-1 alpha IL-1 beta IL-10 IL-12 p70 IL-13 IL-15 IL-16 IL-2 IL-3 IL-4 IL-5 IL-6 IL-7 IL-8 IP-10 LIF LIGHT Leptin MCP-1 MCP-2 MCP-3 MCP-4 MCSF MDC MIF MIG MIP-1 beta MIP-1delta MIP-3 alpha NAP-2 NEG NT-3 NT-4 Oncostatin M Osteopontin Osteoprotegerin PARC PDGF-BB PIGF POS RANTES SCF SDF-1 TARC TGF-beta 1 TGF-beta 2 TGF-beta 3 TIMP-1 TIMP-2 TNF-alpha TNF-beta Thrombopoietin VEGF METABOLITES_END #END