#METABOLOMICS WORKBENCH jgooding_20170227_142647 DATATRACK_ID:855 STUDY_ID:ST000565 ANALYSIS_ID:AN000868 PROJECT_ID:PR000415 VERSION 1 CREATED_ON March 6, 2017, 11:44 am #PROJECT PR:PROJECT_TITLE Association of hemodialysis patient plasma trace metals with response to PR:PROJECT_TITLE erythropoiesis stimulating agents PR:PROJECT_TYPE Metallomics PR:PROJECT_SUMMARY Erythropoietin (EPO) is a cytokine used to treat the anemia of chronic renal PR:PROJECT_SUMMARY disease in greater than 90% of all in-center hemodialysis patients at a cost of PR:PROJECT_SUMMARY approximately 2 billion dollars per year (2010). Despite protocols for anemia PR:PROJECT_SUMMARY management in the end stage kidney disease (ESKD) population, a large proportion PR:PROJECT_SUMMARY of patients do not predictably respond to typical doses of EPO. Several recent PR:PROJECT_SUMMARY randomized controlled trials looking to increase hemoglobin in patients with the PR:PROJECT_SUMMARY anemia of renal disease have uncovered many questions about the treatment of PR:PROJECT_SUMMARY anemia with erythropoietic stimulating agents (ESA) not previously addressed in PR:PROJECT_SUMMARY new drug applications or in subsequent research. We sought to test the PR:PROJECT_SUMMARY hypothesis that serum trace metal concentrations correlate with markers of PR:PROJECT_SUMMARY anemia and response to ESA treatment. EDTA-Plasma from 110 hemodialysis patients PR:PROJECT_SUMMARY participating in an NIDDK funded study (R01-01DK091584) were analyzed by ICP-MS PR:PROJECT_SUMMARY for the concentration of As, Cd, Co, Cr, Cu, Mn, Mo, Ni, Pb, Sb, Se, Sn, V, and PR:PROJECT_SUMMARY Zn. Associations were determined between trace metals and gender, race, PR:PROJECT_SUMMARY hemodialysis status, hemoglobin at the time of draw (Hgb), total ESA dose for PR:PROJECT_SUMMARY the month the sample was collected (EPO), and erythropoietin resistance index PR:PROJECT_SUMMARY determined over the 6 months of treatment leading up to sample collection (ERI). PR:INSTITUTE University of Louisville PR:DEPARTMENT School of Medicine, Division of Nephrology & Hypertension PR:LAST_NAME Merchant PR:FIRST_NAME Michael PR:ADDRESS 550 South Jackson Street, 3rd Floor, Louisville, Kentucky 40202 PR:EMAIL michael.merchant@louisville.edu PR:PHONE 502/852-0245 PR:FUNDING_SOURCE NIH Grants 1R01DK091584, U24DK097193, and K01GM109320 #STUDY ST:STUDY_TITLE Association of hemodialysis patient plasma trace metals with response to ST:STUDY_TITLE erythropoiesis stimulating agents ST:STUDY_TYPE Metallomics ST:STUDY_SUMMARY EDTA-Plasma from 110 hemodialysis patients participating in an NIDDK funded ST:STUDY_SUMMARY study were analyzed by ICP-MS for the concentration of As, Cd, Co, Cr, Cu, Mn, ST:STUDY_SUMMARY Mo, Ni, Pb, Sb, Se, Sn, V, and Zn. Associations were determined between trace ST:STUDY_SUMMARY metals and gender, race, hemodialysis status, hemoglobin at the time of draw ST:STUDY_SUMMARY (Hgb), total ESA dose for the month the sample was collected (EPO), and ST:STUDY_SUMMARY erythropoietin resistance index determined over the 6 months of treatment ST:STUDY_SUMMARY leading up to sample collection (ERI) ST:INSTITUTE RTI International ST:LABORATORY NIH Eastern Regional Comprehensive Metabolomics Resource Core at UNC Chapel Hill ST:LABORATORY (ERCMRC) ST:LAST_NAME Sumner ST:FIRST_NAME Susan ST:ADDRESS 3040 E. Cornwallis Road, Research Triangle Park, NC 27709, USA ST:EMAIL susan_sumner@unc.edu ST:PHONE 704-250-5000 ST:TOTAL_SUBJECTS 110 #SUBJECT SU:SUBJECT_TYPE Human SU:SUBJECT_SPECIES Homo sapiens SU:TAXONOMY_ID 9606 SU:GENDER Both SU:HUMAN_TRIAL_TYPE validation/discovery trial SU:HUMAN_INCLUSION_CRITERIA All subjects will receive hemodialysis in-center on a 3 times per week dialysis SU:HUMAN_INCLUSION_CRITERIA schedule. Subjects are eligible to participate if 1) subjects that have received SU:HUMAN_INCLUSION_CRITERIA EPO will have received it for 6 or more months, 2) between the ages of 18 and 80 SU:HUMAN_INCLUSION_CRITERIA years, 3) have an adequacy of dialysis (Kt/V) that is ≥ 1.2, 4) have adequate SU:HUMAN_INCLUSION_CRITERIA iron stores as measured by ferritin and transferrin saturation. SU:HUMAN_EXCLUSION_CRITERIA None #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS - M1 Gender:F | Race:Caucasian Months on dialysis=14; EPO=14000; ERI=1.69; Hb=11.9; Raw Data Folder and Date of Analysis=Merchant_010616B SUBJECT_SAMPLE_FACTORS - M10 Gender:M | Race:Other Months on dialysis=96; EPO=29200; ERI=3.96; Hb=10.7; Raw Data Folder and Date of Analysis=Merchant_010616B SUBJECT_SAMPLE_FACTORS - M100 Gender:M | Race:African American Months on dialysis=29; EPO=25400; ERI=2.98; Hb=9.6; Raw Data Folder and Date of Analysis=Merchant_011916 SUBJECT_SAMPLE_FACTORS - M101 Gender:F | Race:Other Months on dialysis=32; EPO=88500; ERI=4; Hb=10.1; Raw Data Folder and Date of Analysis=Merchant_011916 SUBJECT_SAMPLE_FACTORS - M102 Gender:M | Race:Caucasian Months on dialysis=1; EPO=-; ERI=-; Hb=10.2; Raw Data Folder and Date of Analysis=Merchant_011916 SUBJECT_SAMPLE_FACTORS - M103 Gender:M | Race:Caucasian Months on dialysis=37; EPO=26000; ERI=2.25; Hb=11.1; Raw Data Folder and Date of Analysis=Merchant_011916 SUBJECT_SAMPLE_FACTORS - M104 Gender:M | Race:Caucasian Months on dialysis=1; EPO=-; ERI=-; Hb=11.8; Raw Data Folder and Date of Analysis=Merchant_011916 SUBJECT_SAMPLE_FACTORS - M105 Gender:F | Race:Caucasian Months on dialysis=101; EPO=115500; ERI=9.1; Hb=7.3; Raw Data Folder and Date of Analysis=Merchant_011916 SUBJECT_SAMPLE_FACTORS - M106 Gender:F | Race:Caucasian Months on dialysis=10; EPO=132000; ERI=7.55; Hb=10; Raw Data Folder and Date of Analysis=Merchant_011916 SUBJECT_SAMPLE_FACTORS - M107 Gender:F | Race:Caucasian Months on dialysis=49; EPO=87100; ERI=7.47; Hb=11.5; Raw Data Folder and Date of Analysis=Merchant_011916 SUBJECT_SAMPLE_FACTORS - M109 Gender:M | Race:Caucasian Months on dialysis=6; EPO=10000; ERI=2.6; Hb=7.9; Raw Data Folder and Date of Analysis=Merchant_011916 SUBJECT_SAMPLE_FACTORS - M11 Gender:M | Race:Caucasian Months on dialysis=6; EPO=-; ERI=-; Hb=10.7; Raw Data Folder and Date of Analysis=Merchant_010616B SUBJECT_SAMPLE_FACTORS - M110 Gender:M | Race:African American Months on dialysis=2; EPO=1000; ERI=0.02; Hb=9.5; Raw Data Folder and Date of Analysis=Merchant_011916 SUBJECT_SAMPLE_FACTORS - M111 Gender:M | Race:Caucasian Months on dialysis=41; EPO=89500; ERI=7.64; Hb=8.7; Raw Data Folder and Date of Analysis=Merchant_011916 SUBJECT_SAMPLE_FACTORS - M112 Gender:M | Race:Caucasian Months on dialysis=6; EPO=30000; ERI=0.74; Hb=12.6; Raw Data Folder and Date of Analysis=Merchant_011916 SUBJECT_SAMPLE_FACTORS - M113 Gender:M | Race:Other Months on dialysis=67; EPO=28800; ERI=1.66; Hb=10.6; Raw Data Folder and Date of Analysis=Merchant_011916 SUBJECT_SAMPLE_FACTORS - M114 Gender:F | Race:Other Months on dialysis=13; EPO=75300; ERI=5.19; Hb=10.3; Raw Data Folder and Date of Analysis=Merchant_011916 SUBJECT_SAMPLE_FACTORS - M115 Gender:M | Race:Caucasian Months on dialysis=19; EPO=-; ERI=2.36; Hb=11.4; Raw Data Folder and Date of Analysis=Merchant_011916 SUBJECT_SAMPLE_FACTORS - M12 Gender:M | Race:Caucasian Months on dialysis=2; EPO=-; ERI=-; Hb=11.8; Raw Data Folder and Date of Analysis=Merchant_010616B SUBJECT_SAMPLE_FACTORS - M13 Gender:M | Race:Other Months on dialysis=1; EPO=6000; ERI=0.26; Hb=7.7; Raw Data Folder and Date of Analysis=Merchant_010616B SUBJECT_SAMPLE_FACTORS - M14 Gender:M | Race:Caucasian Months on dialysis=41; EPO=3200; ERI=1.09; Hb=11.5; Raw Data Folder and Date of Analysis=Merchant_010616B SUBJECT_SAMPLE_FACTORS - M15 Gender:F | Race:Caucasian Months on dialysis=23; EPO=106500; ERI=6.95; Hb=11.1; Raw Data Folder and Date of Analysis=Merchant_010616B SUBJECT_SAMPLE_FACTORS - M16 Gender:M | Race:African American Months on dialysis=8; EPO=14000; ERI=4.14; Hb=10.7; Raw Data Folder and Date of Analysis=Merchant_010616B SUBJECT_SAMPLE_FACTORS - M17 Gender:M | Race:Caucasian Months on dialysis=30; EPO=56000; ERI=9.46; Hb=10.9; Raw Data Folder and Date of Analysis=Merchant_010616B SUBJECT_SAMPLE_FACTORS - M18 Gender:M | Race:Other Months on dialysis=12; EPO=-; ERI=-; Hb=11.8; Raw Data Folder and Date of Analysis=Merchant_010616B SUBJECT_SAMPLE_FACTORS - M19 Gender:M | Race:African American Months on dialysis=12; EPO=-; ERI=-; Hb=13.3; Raw Data Folder and Date of Analysis=Merchant_010616B SUBJECT_SAMPLE_FACTORS - M2 Gender:M | Race:Caucasian Months on dialysis=48; EPO=39800; ERI=3.83; Hb=8.9; Raw Data Folder and Date of Analysis=Merchant_010616B SUBJECT_SAMPLE_FACTORS - M20 Gender:Pool | Race:Pool Months on dialysis=Pool; EPO=Pool; ERI=Pool; Hb=Pool; Raw Data Folder and Date of Analysis=Merchant_010616B SUBJECT_SAMPLE_FACTORS - M21 Gender:M | Race:Other Months on dialysis=31; EPO=18900; ERI=2.21; Hb=10.7; Raw Data Folder and Date of Analysis=Merchant_010616B SUBJECT_SAMPLE_FACTORS - M22 Gender:M | Race:Caucasian Months on dialysis=1; EPO=-; ERI=-; Hb=8.3; Raw Data Folder and Date of Analysis=Merchant_010616B SUBJECT_SAMPLE_FACTORS - M23 Gender:M | Race:Other Months on dialysis=58; EPO=37500; ERI=1.89; Hb=11.5; Raw Data Folder and Date of Analysis=Merchant_010616B SUBJECT_SAMPLE_FACTORS - M24 Gender:M | Race:African American Months on dialysis=1; EPO=5000; ERI=0.2; Hb=8.5; Raw Data Folder and Date of Analysis=Merchant_010616B SUBJECT_SAMPLE_FACTORS - M25 Gender:M | Race:Caucasian Months on dialysis=46; EPO=26900; ERI=2.6; Hb=10.8; Raw Data Folder and Date of Analysis=Merchant_010616B SUBJECT_SAMPLE_FACTORS - M26 Gender:F | Race:Caucasian Months on dialysis=36; EPO=35000; ERI=2.77; Hb=11.2; Raw Data Folder and Date of Analysis=Merchant_010616B SUBJECT_SAMPLE_FACTORS - M27 Gender:M | Race:Caucasian Months on dialysis=8; EPO=-; ERI=1.24; Hb=11.6; Raw Data Folder and Date of Analysis=Merchant_010616B SUBJECT_SAMPLE_FACTORS - M28 Gender:F | Race:Other Months on dialysis=36; EPO=34000; ERI=2.14; Hb=10.3; Raw Data Folder and Date of Analysis=Merchant_010616B SUBJECT_SAMPLE_FACTORS - M29 Gender:M | Race:Caucasian Months on dialysis=9; EPO=22500; ERI=1.36; Hb=12.4; Raw Data Folder and Date of Analysis=Merchant_010616B SUBJECT_SAMPLE_FACTORS - M3 Gender:F | Race:Caucasian Months on dialysis=10; EPO=4000; ERI=2.26; Hb=11.3; Raw Data Folder and Date of Analysis=Merchant_010616B SUBJECT_SAMPLE_FACTORS - M30 Gender:M | Race:Caucasian Months on dialysis=1; EPO=-; ERI=-; Hb=9.1; Raw Data Folder and Date of Analysis=Merchant_010616B SUBJECT_SAMPLE_FACTORS - M31 Gender:M | Race:Caucasian Months on dialysis=46; EPO=-; ERI=0.19; Hb=12.4; Raw Data Folder and Date of Analysis=Merchant_010616B SUBJECT_SAMPLE_FACTORS - M32 Gender:M | Race:African American Months on dialysis=6; EPO=-; ERI=-; Hb=12.6; Raw Data Folder and Date of Analysis=Merchant_010616B SUBJECT_SAMPLE_FACTORS - M33 Gender:F | Race:African American Months on dialysis=1; EPO=-; ERI=-; Hb=9.9; Raw Data Folder and Date of Analysis=Merchant_010616B SUBJECT_SAMPLE_FACTORS - M34 Gender:F | Race:Caucasian Months on dialysis=1; EPO=-; ERI=-; Hb=-; Raw Data Folder and Date of Analysis=Merchant_010616B SUBJECT_SAMPLE_FACTORS - M35 Gender:F | Race:Caucasian Months on dialysis=90; EPO=77000; ERI=6.86; Hb=10.4; Raw Data Folder and Date of Analysis=Merchant_010616B SUBJECT_SAMPLE_FACTORS - M36 Gender:M | Race:Other Months on dialysis=11; EPO=12400; ERI=5.36; Hb=11.9; Raw Data Folder and Date of Analysis=Merchant_010616B SUBJECT_SAMPLE_FACTORS - M37 Gender:M | Race:Caucasian Months on dialysis=49; EPO=39200; ERI=2.4; Hb=10.1; Raw Data Folder and Date of Analysis=Merchant_011116C SUBJECT_SAMPLE_FACTORS - M38 Gender:F | Race:Caucasian Months on dialysis=42; EPO=3000; ERI=0.05; Hb=10.5; Raw Data Folder and Date of Analysis=Merchant_011116C SUBJECT_SAMPLE_FACTORS - M39 Gender:F | Race:Caucasian Months on dialysis=50; EPO=11700; ERI=1.21; Hb=10.1; Raw Data Folder and Date of Analysis=Merchant_011116C SUBJECT_SAMPLE_FACTORS - M4 Gender:F | Race:Caucasian Months on dialysis=26; EPO=58500; ERI=4.95; Hb=10.8; Raw Data Folder and Date of Analysis=Merchant_010616B SUBJECT_SAMPLE_FACTORS - M40 Gender:M | Race:Caucasian Months on dialysis=14; EPO=30000; ERI=4.8; Hb=8.9; Raw Data Folder and Date of Analysis=Merchant_011116C SUBJECT_SAMPLE_FACTORS - M41 Gender:M | Race:Caucasian Months on dialysis=1; EPO=-; ERI=-; Hb=9.9; Raw Data Folder and Date of Analysis=Merchant_011116C SUBJECT_SAMPLE_FACTORS - M42 Gender:M | Race:Caucasian Months on dialysis=197; EPO=17600; ERI=1.52; Hb=10.5; Raw Data Folder and Date of Analysis=Merchant_011116C SUBJECT_SAMPLE_FACTORS - M43 Gender:M | Race:Other Months on dialysis=2; EPO=-; ERI=-; Hb=11.4; Raw Data Folder and Date of Analysis=Merchant_011116C SUBJECT_SAMPLE_FACTORS - M44 Gender:F | Race:Caucasian Months on dialysis=16; EPO=-; ERI=5.53; Hb=9.1; Raw Data Folder and Date of Analysis=Merchant_011116C SUBJECT_SAMPLE_FACTORS - M45 Gender:F | Race:Caucasian Months on dialysis=21; EPO=57600; ERI=4.74; Hb=10.7; Raw Data Folder and Date of Analysis=Merchant_011116C SUBJECT_SAMPLE_FACTORS - M46 Gender:M | Race:Caucasian Months on dialysis=35; EPO=-; ERI=-; Hb=11.5; Raw Data Folder and Date of Analysis=Merchant_011116C SUBJECT_SAMPLE_FACTORS - M47 Gender:F | Race:Caucasian Months on dialysis=1; EPO=-; ERI=-; Hb=-; Raw Data Folder and Date of Analysis=Merchant_011116C SUBJECT_SAMPLE_FACTORS - M48 Gender:M | Race:African American Months on dialysis=1; EPO=-; ERI=-; Hb=8.1; Raw Data Folder and Date of Analysis=Merchant_011116C SUBJECT_SAMPLE_FACTORS - M49 Gender:M | Race:Caucasian Months on dialysis=50; EPO=1800; ERI=0.43; Hb=11.2; Raw Data Folder and Date of Analysis=Merchant_011116C SUBJECT_SAMPLE_FACTORS - M5 Gender:M | Race:Caucasian Months on dialysis=1; EPO=-; ERI=-; Hb=13.2; Raw Data Folder and Date of Analysis=Merchant_010616B SUBJECT_SAMPLE_FACTORS - M50 Gender:M | Race:Caucasian Months on dialysis=1; EPO=-; ERI=-; Hb=10.7; Raw Data Folder and Date of Analysis=Merchant_011116C SUBJECT_SAMPLE_FACTORS - M51 Gender:M | Race:Caucasian Months on dialysis=34; EPO=69000; ERI=4.42; Hb=9.7; Raw Data Folder and Date of Analysis=Merchant_011116C SUBJECT_SAMPLE_FACTORS - M52 Gender:F | Race:Caucasian Months on dialysis=56; EPO=18000; ERI=2.21; Hb=11.7; Raw Data Folder and Date of Analysis=Merchant_011116C SUBJECT_SAMPLE_FACTORS - M53 Gender:M | Race:Caucasian Months on dialysis=24; EPO=26300; ERI=5.49; Hb=10.8; Raw Data Folder and Date of Analysis=Merchant_011116C SUBJECT_SAMPLE_FACTORS - M54 Gender:M | Race:Caucasian Months on dialysis=7; EPO=110000; ERI=11.81; Hb=10.1; Raw Data Folder and Date of Analysis=Merchant_011116C SUBJECT_SAMPLE_FACTORS - M55 Gender:F | Race:Caucasian Months on dialysis=15; EPO=78000; ERI=5.77; Hb=10.6; Raw Data Folder and Date of Analysis=Merchant_011116C SUBJECT_SAMPLE_FACTORS - M56 Gender:M | Race:Caucasian Months on dialysis=1; EPO=-; ERI=-; Hb=9.1; Raw Data Folder and Date of Analysis=Merchant_011116C SUBJECT_SAMPLE_FACTORS - M57 Gender:M | Race:African American Months on dialysis=9; EPO=12000; ERI=1.29; Hb=10.3; Raw Data Folder and Date of Analysis=Merchant_011116C SUBJECT_SAMPLE_FACTORS - M58 Gender:F | Race:Caucasian Months on dialysis=23; EPO=-; ERI=0.83; Hb=11.3; Raw Data Folder and Date of Analysis=Merchant_011116C SUBJECT_SAMPLE_FACTORS - M6 Gender:M | Race:Caucasian Months on dialysis=68; EPO=1300; ERI=1.93; Hb=11.5; Raw Data Folder and Date of Analysis=Merchant_010616B SUBJECT_SAMPLE_FACTORS - M61 Gender:F | Race:Caucasian Months on dialysis=50; EPO=30000; ERI=6.89; Hb=7.4; Raw Data Folder and Date of Analysis=Merchant_011116C SUBJECT_SAMPLE_FACTORS - M62 Gender:M | Race:Other Months on dialysis=98; EPO=40000; ERI=3.03; Hb=10.8; Raw Data Folder and Date of Analysis=Merchant_011116C SUBJECT_SAMPLE_FACTORS - M63 Gender:M | Race:Caucasian Months on dialysis=11; EPO=-; ERI=-; Hb=12; Raw Data Folder and Date of Analysis=Merchant_011116C SUBJECT_SAMPLE_FACTORS - M64 Gender:M | Race:Caucasian Months on dialysis=1; EPO=-; ERI=-; Hb=9.1; Raw Data Folder and Date of Analysis=Merchant_011116C SUBJECT_SAMPLE_FACTORS - M65 Gender:F | Race:Caucasian Months on dialysis=6; EPO=24000; ERI=0.37; Hb=11.3; Raw Data Folder and Date of Analysis=Merchant_011116C SUBJECT_SAMPLE_FACTORS - M66 Gender:M | Race:Caucasian Months on dialysis=1; EPO=-; ERI=-; Hb=11.7; Raw Data Folder and Date of Analysis=Merchant_011116C SUBJECT_SAMPLE_FACTORS - M67 Gender:M | Race:Caucasian Months on dialysis=44; EPO=5600; ERI=0.84; Hb=11.6; Raw Data Folder and Date of Analysis=Merchant_011116C SUBJECT_SAMPLE_FACTORS - M68 Gender:M | Race:Caucasian Months on dialysis=36; EPO=-; ERI=-; Hb=9.4; Raw Data Folder and Date of Analysis=Merchant_011116C SUBJECT_SAMPLE_FACTORS - M69 Gender:M | Race:Other Months on dialysis=68; EPO=130000; ERI=8.48; Hb=11.7; Raw Data Folder and Date of Analysis=Merchant_011116C SUBJECT_SAMPLE_FACTORS - M7 Gender:F | Race:African American Months on dialysis=2; EPO=-; ERI=-; Hb=11.9; Raw Data Folder and Date of Analysis=Merchant_010616B SUBJECT_SAMPLE_FACTORS - M70 Gender:M | Race:Caucasian Months on dialysis=2; EPO=-; ERI=-; Hb=15.2; Raw Data Folder and Date of Analysis=Merchant_011316 SUBJECT_SAMPLE_FACTORS - M71 Gender:M | Race:Caucasian Months on dialysis=37; EPO=15100; ERI=1.41; Hb=9.1; Raw Data Folder and Date of Analysis=Merchant_011316 SUBJECT_SAMPLE_FACTORS - M72 Gender:F | Race:African American Months on dialysis=1; EPO=6000; ERI=0.25; Hb=8.8; Raw Data Folder and Date of Analysis=Merchant_011316 SUBJECT_SAMPLE_FACTORS - M73 Gender:M | Race:Caucasian Months on dialysis=33; EPO=64400; ERI=4.11; Hb=10.7; Raw Data Folder and Date of Analysis=Merchant_011316 SUBJECT_SAMPLE_FACTORS - M74 Gender:F | Race:Caucasian Months on dialysis=1; EPO=-; ERI=-; Hb=10.6; Raw Data Folder and Date of Analysis=Merchant_011316 SUBJECT_SAMPLE_FACTORS - M75 Gender:M | Race:African American Months on dialysis=63; EPO=-; ERI=-; Hb=14.7; Raw Data Folder and Date of Analysis=Merchant_011316 SUBJECT_SAMPLE_FACTORS - M76 Gender:F | Race:African American Months on dialysis=29; EPO=72000; ERI=4.33; Hb=10.8; Raw Data Folder and Date of Analysis=Merchant_011316 SUBJECT_SAMPLE_FACTORS - M77 Gender:F | Race:Caucasian Months on dialysis=49; EPO=53400; ERI=3.73; Hb=11.5; Raw Data Folder and Date of Analysis=Merchant_011316 SUBJECT_SAMPLE_FACTORS - M78 Gender:F | Race:Caucasian Months on dialysis=18; EPO=58000; ERI=9.61; Hb=9.2; Raw Data Folder and Date of Analysis=Merchant_011316 SUBJECT_SAMPLE_FACTORS - M79 Gender:M | Race:Caucasian Months on dialysis=1; EPO=-; ERI=-; Hb=10.3; Raw Data Folder and Date of Analysis=Merchant_011316 SUBJECT_SAMPLE_FACTORS - M8 Gender:M | Race:African American Months on dialysis=4; EPO=11000; ERI=2.55; Hb=9.8; Raw Data Folder and Date of Analysis=Merchant_010616B SUBJECT_SAMPLE_FACTORS - M80 Gender:F | Race:Caucasian Months on dialysis=20; EPO=124800; ERI=8.39; Hb=10.9; Raw Data Folder and Date of Analysis=Merchant_011916 SUBJECT_SAMPLE_FACTORS - M81 Gender:F | Race:Caucasian Months on dialysis=1; EPO=-; ERI=-; Hb=11.2; Raw Data Folder and Date of Analysis=Merchant_011916 SUBJECT_SAMPLE_FACTORS - M82 Gender:M | Race:African American Months on dialysis=26; EPO=19500; ERI=1.37; Hb=11.5; Raw Data Folder and Date of Analysis=Merchant_011916 SUBJECT_SAMPLE_FACTORS - M83 Gender:M | Race:Caucasian Months on dialysis=12; EPO=70100; ERI=7.36; Hb=10.5; Raw Data Folder and Date of Analysis=Merchant_011916 SUBJECT_SAMPLE_FACTORS - M84 Gender:F | Race:Caucasian Months on dialysis=48; EPO=15300; ERI=3.79; Hb=9.1; Raw Data Folder and Date of Analysis=Merchant_011916 SUBJECT_SAMPLE_FACTORS - M85 Gender:F | Race:Caucasian Months on dialysis=92; EPO=71000; ERI=6.88; Hb=12.3; Raw Data Folder and Date of Analysis=Merchant_011916 SUBJECT_SAMPLE_FACTORS - M86 Gender:F | Race:Other Months on dialysis=19; EPO=11100; ERI=1.2; Hb=11.5; Raw Data Folder and Date of Analysis=Merchant_011916 SUBJECT_SAMPLE_FACTORS - M87 Gender:F | Race:- Months on dialysis=34; EPO=21600; ERI=1.91; Hb=12.4; Raw Data Folder and Date of Analysis=Merchant_011916 SUBJECT_SAMPLE_FACTORS - M88 Gender:M | Race:Caucasian Months on dialysis=1; EPO=-; ERI=-; Hb=11.2; Raw Data Folder and Date of Analysis=Merchant_011916 SUBJECT_SAMPLE_FACTORS - M89 Gender:M | Race:Other Months on dialysis=1; EPO=-; ERI=-; Hb=-; Raw Data Folder and Date of Analysis=Merchant_011916 SUBJECT_SAMPLE_FACTORS - M9 Gender:M | Race:Other Months on dialysis=14; EPO=10000; ERI=0.89; Hb=10.8; Raw Data Folder and Date of Analysis=Merchant_010616B SUBJECT_SAMPLE_FACTORS - M90 Gender:M | Race:Caucasian Months on dialysis=61; EPO=11600; ERI=1.89; Hb=10.9; Raw Data Folder and Date of Analysis=Merchant_011916 SUBJECT_SAMPLE_FACTORS - M91 Gender:F | Race:Caucasian Months on dialysis=3; EPO=60000; ERI=2.53; Hb=8.7; Raw Data Folder and Date of Analysis=Merchant_011916 SUBJECT_SAMPLE_FACTORS - M92 Gender:F | Race:Caucasian Months on dialysis=1; EPO=-; ERI=-; Hb=11.3; Raw Data Folder and Date of Analysis=Merchant_011916 SUBJECT_SAMPLE_FACTORS - M93 Gender:M | Race:Caucasian Months on dialysis=38; EPO=130000; ERI=8.69; Hb=8.5; Raw Data Folder and Date of Analysis=Merchant_011916 SUBJECT_SAMPLE_FACTORS - M94 Gender:F | Race:Caucasian Months on dialysis=115; EPO=120000; ERI=6.68; Hb=-; Raw Data Folder and Date of Analysis=Merchant_011916 SUBJECT_SAMPLE_FACTORS - M95 Gender:F | Race:Caucasian Months on dialysis=29; EPO=25000; ERI=2.25; Hb=8.1; Raw Data Folder and Date of Analysis=Merchant_011916 SUBJECT_SAMPLE_FACTORS - M96 Gender:M | Race:Caucasian Months on dialysis=1; EPO=-; ERI=-; Hb=11; Raw Data Folder and Date of Analysis=Merchant_011916 SUBJECT_SAMPLE_FACTORS - M97 Gender:M | Race:Caucasian Months on dialysis=15; EPO=72000; ERI=10.4; Hb=11.3; Raw Data Folder and Date of Analysis=Merchant_011916 SUBJECT_SAMPLE_FACTORS - M98 Gender:F | Race:Caucasian Months on dialysis=47; EPO=136200; ERI=9.93; Hb=10.9; Raw Data Folder and Date of Analysis=Merchant_011916 SUBJECT_SAMPLE_FACTORS - M99 Gender:Pool | Race:Pool Months on dialysis=Pool; EPO=Pool; ERI=Pool; Hb=Pool; Raw Data Folder and Date of Analysis=Merchant_011916 #COLLECTION CO:COLLECTION_SUMMARY Subjects that passed the screening were asked to donate 20 mL of blood for CO:COLLECTION_SUMMARY analysis following an informed consent process. The blood was processed to CO:COLLECTION_SUMMARY plasma and serum and stored as 0.5mL aliquots at -70 degrees until analyzed. CO:SAMPLE_TYPE Blood CO:ADDITIVES EDTA CO:BLOOD_SERUM_OR_PLASMA Plasma #TREATMENT TR:TREATMENT_SUMMARY NA #SAMPLEPREP SP:SAMPLEPREP_SUMMARY A volume of 150 µL of each EDTA-plasma sample was dispensed into an acid-washed SP:SAMPLEPREP_SUMMARY plastic digestion tube. To each digestion vessel, 300 µL of concentrated HNO3, SP:SAMPLEPREP_SUMMARY 150 µL of concentrated HCl, and 100 µL of H2O2 solution was added. Samples SP:SAMPLEPREP_SUMMARY were loosely capped and placed in the graphite digestion block and heated at a SP:SAMPLEPREP_SUMMARY temperature of 90 °C for 2 hours. At the end of digestion, all samples were SP:SAMPLEPREP_SUMMARY removed from the heating block and allowed to cool to room temperature. In the SP:SAMPLEPREP_SUMMARY clean hood, samples were spiked with a multielement internal standard to provide SP:SAMPLEPREP_SUMMARY a final concentration of 1.0 ng/mL Sc, In, and Pr, and diluted to the final SP:SAMPLEPREP_SUMMARY volume with deionized (DI) H2O. SP:PROCESSING_METHOD Acid Digestion SP:PROCESSING_STORAGE_CONDITIONS 90°C SP:EXTRACT_STORAGE 8°C SP:SAMPLE_SPIKING Sc, In, Pr #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE None CH:INSTRUMENT_NAME Thermo Element 2 ICP-MS CH:COLUMN_NAME None #ANALYSIS AN:ANALYSIS_TYPE MS AN:LABORATORY_NAME RTI AN:OPERATOR_NAME James Harrington AN:SOFTWARE_VERSION Element ICP-MS, Version 3.1.2.242 AN:ACQUISITION_DATE 1/6/16, 1/11/16, 1/13/16, and 1/19/16 #MS MS:MS_COMMENTS - MS:INSTRUMENT_NAME Thermo Element 2 ICP MS:INSTRUMENT_TYPE Sector Field MS:MS_TYPE Other MS:ION_MODE POSITIVE MS:MASS_ACCURACY 7000 (medium resolution) and 10000 (high resolution) MS:DATAFORMAT .dat MS:SCAN_RANGE_MOVERZ 27-209 #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS ng/mL plasma MS_METABOLITE_DATA_START Samples M1 M10 M100 M101 M102 M103 M104 M105 M106 M107 M109 M11 M110 M111 M112 M113 M114 M115 M12 M13 M14 M15 M16 M17 M18 M19 M2 M20 M21 M22 M23 M24 M25 M26 M27 M28 M29 M3 M30 M31 M32 M33 M34 M35 M36 M37 M38 M39 M4 M40 M41 M42 M43 M44 M45 M46 M47 M48 M49 M5 M50 M51 M52 M53 M54 M55 M56 M57 M58 M6 M61 M62 M63 M64 M65 M66 M67 M68 M69 M7 M70 M71 M72 M73 M74 M75 M76 M77 M78 M79 M8 M80 M81 M82 M83 M84 M85 M86 M87 M88 M89 M9 M90 M91 M92 M93 M94 M95 M96 M97 M98 M99 Factors Gender:F | Race:Caucasian Gender:M | Race:Other Gender:M | Race:African American Gender:F | Race:Other Gender:M | Race:Caucasian Gender:M | Race:Caucasian Gender:M | Race:Caucasian Gender:F | Race:Caucasian Gender:F | Race:Caucasian Gender:F | Race:Caucasian Gender:M | Race:Caucasian Gender:M | Race:Caucasian Gender:M | Race:African American Gender:M | Race:Caucasian Gender:M | Race:Caucasian Gender:M | Race:Other Gender:F | Race:Other Gender:M | Race:Caucasian Gender:M | Race:Caucasian Gender:M | Race:Other Gender:M | Race:Caucasian Gender:F | Race:Caucasian Gender:M | Race:African American Gender:M | Race:Caucasian Gender:M | Race:Other Gender:M | Race:African American Gender:M | Race:Caucasian Gender:Pool | Race:Pool Gender:M | Race:Other Gender:M | Race:Caucasian Gender:M | Race:Other Gender:M | Race:African American Gender:M | Race:Caucasian Gender:F | Race:Caucasian Gender:M | Race:Caucasian Gender:F | Race:Other Gender:M | Race:Caucasian Gender:F | Race:Caucasian Gender:M | Race:Caucasian Gender:M | Race:Caucasian Gender:M | Race:African American Gender:F | Race:African American Gender:F | Race:Caucasian Gender:F | Race:Caucasian Gender:M | Race:Other Gender:M | Race:Caucasian Gender:F | Race:Caucasian Gender:F | Race:Caucasian Gender:F | Race:Caucasian Gender:M | Race:Caucasian Gender:M | Race:Caucasian Gender:M | Race:Caucasian Gender:M | Race:Other Gender:F | Race:Caucasian Gender:F | Race:Caucasian Gender:M | Race:Caucasian Gender:F | Race:Caucasian Gender:M | Race:African American Gender:M | Race:Caucasian Gender:M | Race:Caucasian Gender:M | Race:Caucasian Gender:M | Race:Caucasian Gender:F | Race:Caucasian Gender:M | Race:Caucasian Gender:M | Race:Caucasian Gender:F | Race:Caucasian Gender:M | Race:Caucasian Gender:M | Race:African American Gender:F | Race:Caucasian Gender:M | Race:Caucasian Gender:F | Race:Caucasian Gender:M | Race:Other Gender:M | Race:Caucasian Gender:M | Race:Caucasian Gender:F | Race:Caucasian Gender:M | Race:Caucasian Gender:M | Race:Caucasian Gender:M | Race:Caucasian Gender:M | Race:Other Gender:F | Race:African American Gender:M | Race:Caucasian Gender:M | Race:Caucasian Gender:F | Race:African American Gender:M | Race:Caucasian Gender:F | Race:Caucasian Gender:M | Race:African American Gender:F | Race:African American Gender:F | Race:Caucasian Gender:F | Race:Caucasian Gender:M | Race:Caucasian Gender:M | Race:African American Gender:F | Race:Caucasian Gender:F | Race:Caucasian Gender:M | Race:African American Gender:M | Race:Caucasian Gender:F | Race:Caucasian Gender:F | Race:Caucasian Gender:F | Race:Other Gender:F | Race:- Gender:M | Race:Caucasian Gender:M | Race:Other Gender:M | Race:Other Gender:M | Race:Caucasian Gender:F | Race:Caucasian Gender:F | Race:Caucasian Gender:M | Race:Caucasian Gender:F | Race:Caucasian Gender:F | Race:Caucasian Gender:M | Race:Caucasian Gender:M | Race:Caucasian Gender:F | Race:Caucasian Gender:Pool | Race:Pool V 1.86 1.62 2.15 1.94 1.33 0.778 1.12 0.947 2.68 1.32 1.05 1.03 1.3 2.52 3.08 1.09 1.13 1.24 1.29 1.68 2.15 1.15 1.53 0.8 2.46 1.19 2.34 0.819 2.05 2.35 1.07 2.24 1.23 1.74 1.73 2.15 0.673 0.96 1.06 0.981 0.968 1.08 2 2.69 1.74 1.42 1.94 0.869 1.7 1.58 1.45 0.8 1.65 1.02 2.66 2.74 2.15 2.67 0.61 1.4 1.25 2.22 2.16 1.95 1.31 0.655 2.43 0.916 0.847 1.35 1.98 0.958 Cr 3.13 4.06 9.82 2.22 0.517 6.17 6.91 4.54 5.2 5.81 14 7.6 0.588 3.8 6.04 27.3 5.37 18 20.5 11.8 2.47 1.56 1.71 12 12.3 14.9 3.7 10.7 0.581 2.14 21 3.01 13 3.45 29.9 13.9 12.6 18.1 15 10.4 6.75 22.4 3.9 2.11 2.75 0.48 3.28 10.5 0.423 11.6 4.01 2.84 2.32 0.512 11.3 0.521 1.54 24.6 2.85 11.2 0.627 2.74 5.63 2.52 2.83 17.4 0.637 4.67 3.9 Mn 0.643 0.695 2.7 2.21 1.27 2.13 1.58 2.13 2.15 0.514 0.358 0.372 0.445 2.54 2.58 0.498 2.9 2.27 0.808 0.355 0.452 0.79 1.2 2.53 0.429 2.53 0.376 0.59 0.42 0.461 0.626 0.385 2.49 0.456 0.401 2.48 2.44 2.56 0.677 0.796 0.79 1.25 1.71 0.943 2.72 1.43 2.87 2.73 0.726 0.86 3.46 2.88 0.617 3.86 2.63 2.92 0.735 0.593 0.608 0.615 6.15 0.575 0.365 0.603 1.26 2.75 3.28 0.697 2.63 0.827 0.601 2.49 2.99 3.76 2.55 2.79 2.48 2.63 2.34 2.67 1.44 1.1 1.17 2.55 0.359 2.87 2.8 0.453 0.712 6.28 2.06 0.745 2.58 0.497 4.09 1.57 Co 0.452 0.59 2.4 0.452 0.412 0.446 0.526 0.48 0.393 0.525 0.537 0.41 0.399 0.402 0.417 0.885 0.748 0.468 0.399 0.581 0.557 0.659 0.502 0.639 0.639 0.633 0.57 0.589 0.528 0.471 0.589 0.45 0.506 0.466 0.445 1.25 0.515 0.516 0.505 0.606 Ni 3.5 3.37 1.53 1.69 1.96 1.72 1.49 0.996 1.46 2.27 0.802 1.14 1.89 2.53 2.21 1.26 0.768 1.64 3.01 2.03 3.32 1.52 4.65 2.14 1.94 1.73 0.779 3.28 1.03 1.55 2.23 1.67 3.34 1.71 2.71 1.59 1.62 1 2.54 2.44 2.02 2.24 1.61 1.71 2.8 2.8 3.98 0.896 2.09 1.09 1.64 1.97 1.51 1.13 1.23 2.95 2.18 3.48 2.51 2.29 1.7 2.35 1.86 1.22 1.76 2.35 4.07 1.99 2.43 0.78 2.37 2.61 2.08 2.09 0.832 0.752 0.94 2.42 1.22 2.77 0.864 1.19 1.36 1.69 1.88 3.82 3.12 2.58 3.21 2.15 1.64 1.81 1.19 1.81 2.37 1.55 2.39 6.53 2.03 Cu 915 908 531 1140 1030 758 898 777 930 605 960 658 552 875 829 711 1090 752 578 944 747 885 602 1060 570 865 940 578 523 1010 613 779 631 833 788 751 759 853 1050 695 451 814 818 568 593 1110 780 734 785 506 1290 630 671 508 834 541 864 849 1180 807 938 295 696 1010 978 818 711 1000 1030 922 656 725 706 1280 921 766 625 595 618 1250 724 729 662 1200 1440 992 1360 1000 1030 633 659 609 754 592 658 620 737 652 372 1000 458 933 523 690 760 927 795 1040 847 631 554 1020 Zn 538 584 646 441 457 503 459 487 494 566 731 825 590 575 543 522 534 405 460 717 676 499 469 483 644 705 595 449 604 429 598 515 552 640 437 615 710 590 465 591 569 524 648 528 570 627 569 521 528 752 657 757 519 549 478 530 578 530 639 528 593 647 494 549 520 543 785 823 917 451 746 601 463 555 579 559 688 587 678 563 610 812 1170 1210 1120 951 1300 611 654 756 1160 515 447 614 537 454 593 408 486 675 449 791 570 408 497 514 505 701 Mo 6.65 2.95 1.67 5.64 1.21 5.32 1.85 2.91 3.52 2.04 6.83 7.33 4.48 2.1 3.27 5.57 5.28 3.02 1.59 4.09 3.3 3.1 3.81 1.62 5.77 2.73 5.19 2.76 3.77 10.5 4.26 5 7.49 2.51 3.22 4.67 2.22 1.51 2.95 1.87 2.89 3.78 1.79 7.1 3.46 12.7 5.66 4.04 5.67 0.874 2.18 2.6 2.4 7.02 1.7 2.4 1.48 7.41 3.34 2.48 1.3 0.97 1.12 5.54 6.45 2.45 5.7 3.97 1.39 3.42 4.44 0.848 1.69 3.26 2.73 3.17 4.62 4.51 3.24 1.94 4.96 6.77 4.28 4.22 7.04 1.96 2.48 7.5 10.4 7.68 4.09 3.98 4.02 3.92 3.41 9.71 5.67 3.72 4.05 3.86 5.3 2.08 6.82 2.21 2.67 8.24 1.21 1.85 7.09 5.1 Cd 0.141 0.212 0.198 0.138 0.274 0.109 0.233 0.161 0.27 0.235 0.2 0.131 0.143 0.156 0.307 0.27 0.222 0.114 0.204 0.224 0.139 0.116 0.195 0.667 0.196 0.199 0.244 0.319 0.185 0.216 0.141 0.226 0.155 0.214 0.243 0.205 0.179 0.173 0.147 0.281 0.177 0.237 0.224 0.252 0.243 0.325 0.249 0.129 0.249 0.569 0.171 0.171 0.356 0.192 0.264 0.209 0.222 0.246 0.373 0.309 0.279 0.214 0.329 0.22 0.358 0.259 0.217 0.171 0.194 0.203 0.423 0.218 0.22 0.256 0.201 0.182 0.174 0.21 0.266 0.42 0.218 0.24 0.108 0.32 0.239 0.251 Sn 1.59 2.18 4.67 2.16 1.45 2.75 1.62 1.48 4.62 1.7 15.8 1.39 0.842 0.879 2.27 4.28 3.97 1.27 0.757 1.5 1.32 2.65 0.97 2.99 1.69 4.75 2.44 0.492 2.18 5.3 0.61 6.53 1.56 5.39 0.497 2.28 0.609 1.75 0.968 0.86 1.86 1.33 1.14 5.79 2.33 1.12 2.51 0.745 1.3 2.29 1.64 0.444 0.874 5.56 0.847 1.39 1.81 2.68 1.36 1.63 2.44 1.21 1.72 2.5 1.76 10.2 1.18 0.698 4.78 2.06 3.41 1.69 1.26 1.9 0.55 4.59 0.61 2.49 0.867 2.98 6.53 1.21 3.26 7.71 6.05 2.22 1.05 1.09 1.05 8.06 1.03 1.79 1.29 1.83 1.8 1.69 2.64 3.38 0.736 8.45 1.7 2.68 0.977 5.23 2.46 3.32 Sb 4.44 3.78 3.26 3.73 4.01 3.6 3.24 2.46 3.26 3.2 3.02 2.87 4.42 4.09 4.59 3.59 3.75 3.5 2.98 3.39 3.54 4.38 3.58 4.1 4.67 3.65 3.99 2.62 5.62 3.98 3.7 3.32 3.62 4.35 3.09 4.68 3.88 3.92 3.65 4.73 3.7 3.86 3.08 3.68 3.35 4.86 5.06 4.68 3.51 3.42 4 4.46 3.48 2.87 3.69 3.3 3.22 3.85 3.51 6.05 3.84 4.15 3.23 3.54 3.99 3.75 3.68 5.8 4.55 4.17 2.87 3.95 3.49 5.17 4.09 3.88 3.92 2.68 3 3.68 6.83 4.03 4.38 4.56 4.55 6.23 3.9 4.94 4.79 4.5 3.15 3.85 4.49 3.52 4.55 3.46 4.19 3.33 4.42 3.97 3.39 2.78 4.01 3.54 4 3.03 3.07 2.84 4.23 4.24 3.01 4.56 Pb 0.377 0.413 0.864 0.586 0.352 0.452 0.426 0.471 0.403 0.333 0.452 0.747 0.701 0.515 0.32 0.442 0.353 0.416 0.316 0.383 0.384 0.451 0.394 0.493 0.474 0.332 0.338 0.693 0.33 0.396 0.91 0.391 0.404 0.751 0.525 0.621 0.416 0.466 2.08 0.596 0.47 1.85 0.717 0.725 1.02 1.94 As 6.5 6.94 6.5 11 11.7 6.49 7.65 20.9 7.98 11.6 7.83 Se 94.7 126 122 88 98.5 106 102 94.4 104 96.4 107 112 107 104 119 118 105 110 119 109 143 98.1 116 99.8 99.7 120 127 81.1 123 102 126 114 121 140 120 68.6 126 108 91.7 140 93.8 143 139 108 110 93.1 103 119 124 133 114 146 122 132 113 124 136 107 116 85.4 152 73.7 101 127 117 111 129 172 142 92.7 100 118 60.3 98.7 113 108 112 138 145 87.6 179 162 113 148 155 184 151 121 169 154 113 77.4 120 113 126 114 99.1 126 126 105 97 81.8 145 87.4 109 86.1 80.4 117 100 97.9 102 143 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name retention index quantified m/z PubChem ID KEGG ID V 23990 Cr 23976 Mn 23930 Co 104730 Ni 935 Cu 23978 Zn 23994 Mo 23932 Cd 23973 Sn 5352426 Sb 5354495 Pb 5352425 As 5359596 Se 6326970 METABOLITES_END #END