#METABOLOMICS WORKBENCH hormel101_20170623_152446 DATATRACK_ID:1102 STUDY_ID:ST000653 ANALYSIS_ID:AN000993 PROJECT_ID:PR000460
VERSION             	1
CREATED_ON             	June 26, 2017, 8:42 am
#PROJECT
PR:PROJECT_TITLE                 	The dystrophic muscle metabolome: effects of exercise and NO donor therapy
PR:PROJECT_SUMMARY               	"In Duchenne and Becker muscular dystrophy (DMD, BMD), loss of the cytoskeletal
PR:PROJECT_SUMMARY               	protein dystrophin weakens the sarcolemma and disrupts cellular signaling,
PR:PROJECT_SUMMARY               	rendering the diseased muscles susceptible to contractioninduced damage. We and
PR:PROJECT_SUMMARY               	others have shown that loss of neuronal nitric oxide synthase (nNOSμ) from the
PR:PROJECT_SUMMARY               	sarcolemma of dystrophin-deficient muscle causes functional muscle ischemia
PR:PROJECT_SUMMARY               	during exercise due to unopposed sympathetic vasoconstriction, thereby
PR:PROJECT_SUMMARY               	exacerbating fatigue and injury of the diseased muscles. Genetic and
PR:PROJECT_SUMMARY               	pharmacologic strategies targeting nNOSμ-NO signaling ameliorate functional
PR:PROJECT_SUMMARY               	muscle ischemia, as well as many other features of the dystrophic phenotype in
PR:PROJECT_SUMMARY               	the mdx mouse model of DMD/BMD. These findings suggest that the therapeutic
PR:PROJECT_SUMMARY               	benefit of NO likely extends beyond its vascular effects. A growing body of
PR:PROJECT_SUMMARY               	evidence indicates that NO directly influences muscle metabolism through effects
PR:PROJECT_SUMMARY               	on glucose transport as well as mitochondrial biogenesis and function. Both
PR:PROJECT_SUMMARY               	nNOS-/- mice and mdx mice exhibit muscle mitochondrial dysfunction, decreased
PR:PROJECT_SUMMARY               	resistance to fatigue, and exercise-induced muscle injury, suggesting a causal
PR:PROJECT_SUMMARY               	role of nNOSμ-NO deficiency. However, the specific metabolic changes resulting
PR:PROJECT_SUMMARY               	from reduced NO signaling that might render dystrophic muscle susceptible to
PR:PROJECT_SUMMARY               	fatigue and use-dependent injury remain poorly defined. Therefore, the goal of
PR:PROJECT_SUMMARY               	this pilot metabolomics study is to identify the unique biochemical profiles of
PR:PROJECT_SUMMARY               	skeletal and cardiac muscles of mdx mice to gain further mechanistic insight
PR:PROJECT_SUMMARY               	into the pathophysiological role of NO deficiency in muscular dystrophy. In Aim
PR:PROJECT_SUMMARY               	1, we will characterize the skeletal and cardiac muscle metabolomes of mdx and
PR:PROJECT_SUMMARY               	nNOS-/- mice at rest and following a single bout of treadmill exercise with the
PR:PROJECT_SUMMARY               	goal of discovering common metabolic signatures caused by loss of NO signaling.
PR:PROJECT_SUMMARY               	In Aim 2, we will evaluate the potential of a NO donor drug that is under
PR:PROJECT_SUMMARY               	development as a therapeutic for DMD/BMD to improve the skeletal and cardiac
PR:PROJECT_SUMMARY               	muscle metabolomes in mdx mice. As a result of this pilot study, we hope to gain
PR:PROJECT_SUMMARY               	new understanding of the metabolic derangements in dystrophin-deficient muscle,
PR:PROJECT_SUMMARY               	insight into the therapeutic effects of NO replacement, and to identify new
PR:PROJECT_SUMMARY               	pathogenic mechanisms and putative therapeutic targets that will form the basis
PR:PROJECT_SUMMARY               	of future grant applications."
PR:INSTITUTE                     	Mayo Clinic
PR:LAST_NAME                     	Thomas
PR:FIRST_NAME                    	Gail
PR:ADDRESS                       	Penn State Hershey Heart and Vascular Institute Penn State College of Medicine
PR:ADDRESS                       	500 University Drive, MC H047 Hershey, PA 17033
PR:EMAIL                         	gthomas4@hmc.psu.edu
PR:PHONE                         	717-531-0003, ext. 287087
#STUDY
ST:STUDY_TITLE                   	Effects of Exercise on Dystrophic Mouse Heart Meabolome (part IX)
ST:STUDY_SUMMARY                 	We will use male C57BL10, mdx, and nNOS-/- mice (n = 10 each group) to
ST:STUDY_SUMMARY                 	characterize the skeletal and cardiac muscle metabolomes. Half of the mice in
ST:STUDY_SUMMARY                 	each group will remain sedentary while the other half will be subjected to a
ST:STUDY_SUMMARY                 	single bout of treadmill exercise to exhaustion. Mice will be euthanized
ST:STUDY_SUMMARY                 	immediately postexercise and blood, hearts, and hindlimb muscles will be
ST:STUDY_SUMMARY                 	harvested and frozen as detailed in the General Methods. The heart and
ST:STUDY_SUMMARY                 	gastrocnemius, soleus, and quadriceps muscles of one hindlimb will be sent to
ST:STUDY_SUMMARY                 	the Mayo Clinic Metabolomics Resource Core. The heart and quadriceps muscle will
ST:STUDY_SUMMARY                 	be used for untargeted metabolomics profiling (LC/MS) while the gastrocnemius
ST:STUDY_SUMMARY                 	and soleus muscles will be used for targeted analyses of amino acids plus amino
ST:STUDY_SUMMARY                 	metabolites, non-esterified fatty acids, and citric acid cycle intermediates.
ST:INSTITUTE                     	Mayo Clinic
ST:LAST_NAME                     	Thomas
ST:FIRST_NAME                    	Gail
ST:ADDRESS                       	Penn State Hershey Heart and Vascular Institute Penn State College of Medicine
ST:ADDRESS                       	500 University Drive, MC H047 Hershey, PA 17033
ST:EMAIL                         	gthomas4@hmc.psu.edu
ST:PHONE                         	717-531-0003, ext. 287087
#SUBJECT
SU:SUBJECT_TYPE                  	Mouse
SU:SUBJECT_SPECIES               	Mus musculus
SU:TAXONOMY_ID                   	10090
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data
SUBJECT_SAMPLE_FACTORS           	ms5940-1	nC18-18may16-001-r001	Group:SED-nNOS-/- | rep:1	chromatograhy=NC18
SUBJECT_SAMPLE_FACTORS           	ms5940-1	nC18-18may16-001-r002	Group:SED-nNOS-/- | rep:2	chromatograhy=NC18
SUBJECT_SAMPLE_FACTORS           	ms5940-2	nC18-18may16-002-r001	Group:SED-MDX | rep:1	chromatograhy=NC18
SUBJECT_SAMPLE_FACTORS           	ms5940-2	nC18-18may16-002-r002	Group:SED-MDX | rep:2	chromatograhy=NC18
SUBJECT_SAMPLE_FACTORS           	ms5940-3	nC18-18may16-003-r001	Group:RUN-nNOS-/- | rep:1	chromatograhy=NC18
SUBJECT_SAMPLE_FACTORS           	ms5940-3	nC18-18may16-003-r002	Group:RUN-nNOS-/- | rep:2	chromatograhy=NC18
SUBJECT_SAMPLE_FACTORS           	ms5940-4	nC18-18may16-004-r001	Group:RUN-MDX | rep:1	chromatograhy=NC18
SUBJECT_SAMPLE_FACTORS           	ms5940-4	nC18-18may16-004-r002	Group:RUN-MDX | rep:2	chromatograhy=NC18
SUBJECT_SAMPLE_FACTORS           	ms5940-5	nC18-18may16-005-r001	Group:SED-MDX | rep:1	chromatograhy=NC18
SUBJECT_SAMPLE_FACTORS           	ms5940-5	nC18-18may16-005-r002	Group:SED-MDX | rep:2	chromatograhy=NC18
SUBJECT_SAMPLE_FACTORS           	ms5940-6	nC18-18may16-006-r001	Group:SED-BL10 Control | rep:1	chromatograhy=NC18
SUBJECT_SAMPLE_FACTORS           	ms5940-6	nC18-18may16-006-r002	Group:SED-BL10 Control | rep:2	chromatograhy=NC18
SUBJECT_SAMPLE_FACTORS           	ms5940-7	nC18-18may16-007-r001	Group:SED-MDX | rep:1	chromatograhy=NC18
SUBJECT_SAMPLE_FACTORS           	ms5940-7	nC18-18may16-007-r002	Group:SED-MDX | rep:2	chromatograhy=NC18
SUBJECT_SAMPLE_FACTORS           	ms5940-8	nC18-18may16-008-r001	Group:SED-nNOS-/- | rep:1	chromatograhy=NC18
SUBJECT_SAMPLE_FACTORS           	ms5940-8	nC18-18may16-008-r002	Group:SED-nNOS-/- | rep:2	chromatograhy=NC18
SUBJECT_SAMPLE_FACTORS           	ms5940-9	nC18-18may16-009-r001	Group:SED-BL10 Control | rep:1	chromatograhy=NC18
SUBJECT_SAMPLE_FACTORS           	ms5940-9	nC18-18may16-009-r002	Group:SED-BL10 Control | rep:2	chromatograhy=NC18
SUBJECT_SAMPLE_FACTORS           	ms5940-10	nC18-18may16-010-r001	Group:RUN-MDX | rep:1	chromatograhy=NC18
SUBJECT_SAMPLE_FACTORS           	ms5940-10	nC18-18may16-010-r002	Group:RUN-MDX | rep:2	chromatograhy=NC18
SUBJECT_SAMPLE_FACTORS           	ms5940-11	nC18-18may16-011-r001	Group:SED-BL10 Control | rep:1	chromatograhy=NC18
SUBJECT_SAMPLE_FACTORS           	ms5940-11	nC18-18may16-011-r002	Group:SED-BL10 Control | rep:2	chromatograhy=NC18
SUBJECT_SAMPLE_FACTORS           	ms5940-12	nC18-18may16-012-r001	Group:RUN-MDX | rep:1	chromatograhy=NC18
SUBJECT_SAMPLE_FACTORS           	ms5940-12	nC18-18may16-012-r002	Group:RUN-MDX | rep:2	chromatograhy=NC18
SUBJECT_SAMPLE_FACTORS           	ms5940-13	nC18-18may16-013-r001	Group:RUN-BL10 Control | rep:1	chromatograhy=NC18
SUBJECT_SAMPLE_FACTORS           	ms5940-13	nC18-18may16-013-r002	Group:RUN-BL10 Control | rep:2	chromatograhy=NC18
SUBJECT_SAMPLE_FACTORS           	ms5940-14	nC18-18may16-014-r001	Group:SED-nNOS-/- | rep:1	chromatograhy=NC18
SUBJECT_SAMPLE_FACTORS           	ms5940-14	nC18-18may16-014-r002	Group:SED-nNOS-/- | rep:2	chromatograhy=NC18
SUBJECT_SAMPLE_FACTORS           	ms5940-15	nC18-18may16-015-r001	Group:SED-BL10 Control | rep:1	chromatograhy=NC18
SUBJECT_SAMPLE_FACTORS           	ms5940-15	nC18-18may16-015-r002	Group:SED-BL10 Control | rep:2	chromatograhy=NC18
SUBJECT_SAMPLE_FACTORS           	ms5940-16	nC18-18may16-016-r001	Group:RUN-BL10 Control | rep:1	chromatograhy=NC18
SUBJECT_SAMPLE_FACTORS           	ms5940-16	nC18-18may16-016-r002	Group:RUN-BL10 Control | rep:2	chromatograhy=NC18
SUBJECT_SAMPLE_FACTORS           	ms5940-17	nC18-18may16-017-r001	Group:RUN-nNOS-/- | rep:1	chromatograhy=NC18
SUBJECT_SAMPLE_FACTORS           	ms5940-17	nC18-18may16-017-r002	Group:RUN-nNOS-/- | rep:2	chromatograhy=NC18
SUBJECT_SAMPLE_FACTORS           	ms5940-18	nC18-18may16-018-r001	Group:SED-MDX | rep:1	chromatograhy=NC18
SUBJECT_SAMPLE_FACTORS           	ms5940-18	nC18-18may16-018-r002	Group:SED-MDX | rep:2	chromatograhy=NC18
SUBJECT_SAMPLE_FACTORS           	ms5940-19	nC18-18may16-019-r001	Group:RUN-BL10 Control | rep:1	chromatograhy=NC18
SUBJECT_SAMPLE_FACTORS           	ms5940-19	nC18-18may16-019-r002	Group:RUN-BL10 Control | rep:2	chromatograhy=NC18
SUBJECT_SAMPLE_FACTORS           	ms5940-20	nC18-18may16-020-r001	Group:SED-nNOS-/- | rep:1	chromatograhy=NC18
SUBJECT_SAMPLE_FACTORS           	ms5940-20	nC18-18may16-020-r002	Group:SED-nNOS-/- | rep:2	chromatograhy=NC18
SUBJECT_SAMPLE_FACTORS           	ms5940-21	nC18-18may16-021-r001	Group:RUN-BL10 Control | rep:1	chromatograhy=NC18
SUBJECT_SAMPLE_FACTORS           	ms5940-21	nC18-18may16-021-r002	Group:RUN-BL10 Control | rep:2	chromatograhy=NC18
SUBJECT_SAMPLE_FACTORS           	ms5940-22	nC18-18may16-022-r001	Group:RUN-nNOS-/- | rep:1	chromatograhy=NC18
SUBJECT_SAMPLE_FACTORS           	ms5940-22	nC18-18may16-022-r002	Group:RUN-nNOS-/- | rep:2	chromatograhy=NC18
SUBJECT_SAMPLE_FACTORS           	ms5940-23	nC18-18may16-023-r001	Group:RUN-MDX | rep:1	chromatograhy=NC18
SUBJECT_SAMPLE_FACTORS           	ms5940-23	nC18-18may16-023-r002	Group:RUN-MDX | rep:2	chromatograhy=NC18
SUBJECT_SAMPLE_FACTORS           	ms5940-24	nC18-18may16-024-r001	Group:RUN-nNOS-/- | rep:1	chromatograhy=NC18
SUBJECT_SAMPLE_FACTORS           	ms5940-24	nC18-18may16-024-r002	Group:RUN-nNOS-/- | rep:2	chromatograhy=NC18
SUBJECT_SAMPLE_FACTORS           	ms5940-25	nC18-18may16-025-r001	Group:RUN-BL10 Control | rep:1	chromatograhy=NC18
SUBJECT_SAMPLE_FACTORS           	ms5940-25	nC18-18may16-025-r002	Group:RUN-BL10 Control | rep:2	chromatograhy=NC18
SUBJECT_SAMPLE_FACTORS           	ms5940-26	nC18-18may16-026-r001	Group:RUN-nNOS-/- | rep:1	chromatograhy=NC18
SUBJECT_SAMPLE_FACTORS           	ms5940-26	nC18-18may16-026-r002	Group:RUN-nNOS-/- | rep:2	chromatograhy=NC18
SUBJECT_SAMPLE_FACTORS           	ms5940-27	nC18-18may16-027-r001	Group:RUN-MDX | rep:1	chromatograhy=NC18
SUBJECT_SAMPLE_FACTORS           	ms5940-27	nC18-18may16-027-r002	Group:RUN-MDX | rep:2	chromatograhy=NC18
SUBJECT_SAMPLE_FACTORS           	ms5940-28	nC18-18may16-028-r001	Group:SED-nNOS-/- | rep:1	chromatograhy=NC18
SUBJECT_SAMPLE_FACTORS           	ms5940-28	nC18-18may16-028-r002	Group:SED-nNOS-/- | rep:2	chromatograhy=NC18
SUBJECT_SAMPLE_FACTORS           	ms5940-29	nC18-18may16-029-r001	Group:SED-MDX | rep:1	chromatograhy=NC18
SUBJECT_SAMPLE_FACTORS           	ms5940-29	nC18-18may16-029-r002	Group:SED-MDX | rep:2	chromatograhy=NC18
SUBJECT_SAMPLE_FACTORS           	ms5940-30	nC18-18may16-030-r001	Group:SED-BL10 Control | rep:1	chromatograhy=NC18
SUBJECT_SAMPLE_FACTORS           	ms5940-30	nC18-18may16-030-r002	Group:SED-BL10 Control | rep:2	chromatograhy=NC18
SUBJECT_SAMPLE_FACTORS           	ms5940-1	pC18-16may16-001-r001	Group:SED-nNOS-/- | rep:1	chromatograhy=PC18
SUBJECT_SAMPLE_FACTORS           	ms5940-1	pC18-16may16-001-r002	Group:SED-nNOS-/- | rep:2	chromatograhy=PC18
SUBJECT_SAMPLE_FACTORS           	ms5940-2	pC18-16may16-002-r001	Group:SED-MDX | rep:1	chromatograhy=PC18
SUBJECT_SAMPLE_FACTORS           	ms5940-2	pC18-16may16-002-r002	Group:SED-MDX | rep:2	chromatograhy=PC18
SUBJECT_SAMPLE_FACTORS           	ms5940-3	pC18-16may16-003-r001	Group:RUN-nNOS-/- | rep:1	chromatograhy=PC18
SUBJECT_SAMPLE_FACTORS           	ms5940-3	pC18-16may16-003-r002	Group:RUN-nNOS-/- | rep:2	chromatograhy=PC18
SUBJECT_SAMPLE_FACTORS           	ms5940-4	pC18-16may16-004-r001	Group:RUN-MDX | rep:1	chromatograhy=PC18
SUBJECT_SAMPLE_FACTORS           	ms5940-4	pC18-16may16-004-r002	Group:RUN-MDX | rep:2	chromatograhy=PC18
SUBJECT_SAMPLE_FACTORS           	ms5940-5	pC18-16may16-005-r001	Group:SED-MDX | rep:1	chromatograhy=PC18
SUBJECT_SAMPLE_FACTORS           	ms5940-5	pC18-16may16-005-r002	Group:SED-MDX | rep:2	chromatograhy=PC18
SUBJECT_SAMPLE_FACTORS           	ms5940-6	pC18-16may16-006-r001	Group:SED-BL10 Control | rep:1	chromatograhy=PC18
SUBJECT_SAMPLE_FACTORS           	ms5940-6	pC18-16may16-006-r002	Group:SED-BL10 Control | rep:2	chromatograhy=PC18
SUBJECT_SAMPLE_FACTORS           	ms5940-7	pC18-16may16-007-r001	Group:SED-MDX | rep:1	chromatograhy=PC18
SUBJECT_SAMPLE_FACTORS           	ms5940-7	pC18-16may16-007-r002	Group:SED-MDX | rep:2	chromatograhy=PC18
SUBJECT_SAMPLE_FACTORS           	ms5940-8	pC18-16may16-008-r001	Group:SED-nNOS-/- | rep:1	chromatograhy=PC18
SUBJECT_SAMPLE_FACTORS           	ms5940-8	pC18-16may16-008-r002	Group:SED-nNOS-/- | rep:2	chromatograhy=PC18
SUBJECT_SAMPLE_FACTORS           	ms5940-9	pC18-16may16-009-r001	Group:SED-BL10 Control | rep:1	chromatograhy=PC18
SUBJECT_SAMPLE_FACTORS           	ms5940-9	pC18-16may16-009-r002	Group:SED-BL10 Control | rep:2	chromatograhy=PC18
SUBJECT_SAMPLE_FACTORS           	ms5940-10	pC18-16may16-010-r001	Group:RUN-MDX | rep:1	chromatograhy=PC18
SUBJECT_SAMPLE_FACTORS           	ms5940-10	pC18-16may16-010-r002	Group:RUN-MDX | rep:2	chromatograhy=PC18
SUBJECT_SAMPLE_FACTORS           	ms5940-11	pC18-16may16-011-r001	Group:SED-BL10 Control | rep:1	chromatograhy=PC18
SUBJECT_SAMPLE_FACTORS           	ms5940-11	pC18-16may16-011-r002	Group:SED-BL10 Control | rep:2	chromatograhy=PC18
SUBJECT_SAMPLE_FACTORS           	ms5940-12	pC18-16may16-012-r001	Group:RUN-MDX | rep:1	chromatograhy=PC18
SUBJECT_SAMPLE_FACTORS           	ms5940-12	pC18-16may16-012-r002	Group:RUN-MDX | rep:2	chromatograhy=PC18
SUBJECT_SAMPLE_FACTORS           	ms5940-13	pC18-16may16-013-r001	Group:RUN-BL10 Control | rep:1	chromatograhy=PC18
SUBJECT_SAMPLE_FACTORS           	ms5940-13	pC18-16may16-013-r002	Group:RUN-BL10 Control | rep:2	chromatograhy=PC18
SUBJECT_SAMPLE_FACTORS           	ms5940-14	pC18-16may16-014-r001	Group:SED-nNOS-/- | rep:1	chromatograhy=PC18
SUBJECT_SAMPLE_FACTORS           	ms5940-14	pC18-16may16-014-r002	Group:SED-nNOS-/- | rep:2	chromatograhy=PC18
SUBJECT_SAMPLE_FACTORS           	ms5940-15	pC18-16may16-015-r001	Group:SED-BL10 Control | rep:1	chromatograhy=PC18
SUBJECT_SAMPLE_FACTORS           	ms5940-15	pC18-16may16-015-r002	Group:SED-BL10 Control | rep:2	chromatograhy=PC18
SUBJECT_SAMPLE_FACTORS           	ms5940-16	pC18-16may16-016-r001	Group:RUN-BL10 Control | rep:1	chromatograhy=PC18
SUBJECT_SAMPLE_FACTORS           	ms5940-16	pC18-16may16-016-r002	Group:RUN-BL10 Control | rep:2	chromatograhy=PC18
SUBJECT_SAMPLE_FACTORS           	ms5940-17	pC18-16may16-017-r001	Group:RUN-nNOS-/- | rep:1	chromatograhy=PC18
SUBJECT_SAMPLE_FACTORS           	ms5940-17	pC18-16may16-017-r002	Group:RUN-nNOS-/- | rep:2	chromatograhy=PC18
SUBJECT_SAMPLE_FACTORS           	ms5940-18	pC18-16may16-018-r001	Group:SED-MDX | rep:1	chromatograhy=PC18
SUBJECT_SAMPLE_FACTORS           	ms5940-18	pC18-16may16-018-r002	Group:SED-MDX | rep:2	chromatograhy=PC18
SUBJECT_SAMPLE_FACTORS           	ms5940-19	pC18-16may16-019-r001	Group:RUN-BL10 Control | rep:1	chromatograhy=PC18
SUBJECT_SAMPLE_FACTORS           	ms5940-19	pC18-16may16-019-r002	Group:RUN-BL10 Control | rep:2	chromatograhy=PC18
SUBJECT_SAMPLE_FACTORS           	ms5940-20	pC18-16may16-020-r001	Group:SED-nNOS-/- | rep:1	chromatograhy=PC18
SUBJECT_SAMPLE_FACTORS           	ms5940-20	pC18-16may16-020-r002	Group:SED-nNOS-/- | rep:2	chromatograhy=PC18
SUBJECT_SAMPLE_FACTORS           	ms5940-21	pC18-16may16-021-r001	Group:RUN-BL10 Control | rep:1	chromatograhy=PC18
SUBJECT_SAMPLE_FACTORS           	ms5940-21	pC18-16may16-021-r002	Group:RUN-BL10 Control | rep:2	chromatograhy=PC18
SUBJECT_SAMPLE_FACTORS           	ms5940-22	pC18-16may16-022-r001	Group:RUN-nNOS-/- | rep:1	chromatograhy=PC18
SUBJECT_SAMPLE_FACTORS           	ms5940-22	pC18-16may16-022-r002	Group:RUN-nNOS-/- | rep:2	chromatograhy=PC18
SUBJECT_SAMPLE_FACTORS           	ms5940-23	pC18-16may16-023-r001	Group:RUN-MDX | rep:1	chromatograhy=PC18
SUBJECT_SAMPLE_FACTORS           	ms5940-23	pC18-16may16-023-r002	Group:RUN-MDX | rep:2	chromatograhy=PC18
SUBJECT_SAMPLE_FACTORS           	ms5940-24	pC18-16may16-024-r001	Group:RUN-nNOS-/- | rep:1	chromatograhy=PC18
SUBJECT_SAMPLE_FACTORS           	ms5940-24	pC18-16may16-024-r002	Group:RUN-nNOS-/- | rep:2	chromatograhy=PC18
SUBJECT_SAMPLE_FACTORS           	ms5940-25	pC18-16may16-025-r001	Group:RUN-BL10 Control | rep:1	chromatograhy=PC18
SUBJECT_SAMPLE_FACTORS           	ms5940-25	pC18-16may16-025-r002	Group:RUN-BL10 Control | rep:2	chromatograhy=PC18
SUBJECT_SAMPLE_FACTORS           	ms5940-26	pC18-16may16-026-r001	Group:RUN-nNOS-/- | rep:1	chromatograhy=PC18
SUBJECT_SAMPLE_FACTORS           	ms5940-26	pC18-16may16-026-r002	Group:RUN-nNOS-/- | rep:2	chromatograhy=PC18
SUBJECT_SAMPLE_FACTORS           	ms5940-27	pC18-16may16-027-r001	Group:RUN-MDX | rep:1	chromatograhy=PC18
SUBJECT_SAMPLE_FACTORS           	ms5940-27	pC18-16may16-027-r002	Group:RUN-MDX | rep:2	chromatograhy=PC18
SUBJECT_SAMPLE_FACTORS           	ms5940-28	pC18-16may16-028-r001	Group:SED-nNOS-/- | rep:1	chromatograhy=PC18
SUBJECT_SAMPLE_FACTORS           	ms5940-28	pC18-16may16-028-r002	Group:SED-nNOS-/- | rep:2	chromatograhy=PC18
SUBJECT_SAMPLE_FACTORS           	ms5940-29	pC18-16may16-029-r001	Group:SED-MDX | rep:1	chromatograhy=PC18
SUBJECT_SAMPLE_FACTORS           	ms5940-29	pC18-16may16-029-r002	Group:SED-MDX | rep:2	chromatograhy=PC18
SUBJECT_SAMPLE_FACTORS           	ms5940-30	pC18-16may16-030-r001	Group:SED-BL10 Control | rep:1	chromatograhy=PC18
SUBJECT_SAMPLE_FACTORS           	ms5940-30	pC18-16may16-030-r002	Group:SED-BL10 Control | rep:2	chromatograhy=PC18
SUBJECT_SAMPLE_FACTORS           	ms5940-1	nhilic-18may16-001-r001	Group:SED-nNOS-/- | rep:1	chromatograhy=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-1	nhilic-18may16-001-r002	Group:SED-nNOS-/- | rep:2	chromatograhy=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-2	nhilic-18may16-002-r001	Group:SED-MDX | rep:1	chromatograhy=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-2	nhilic-18may16-002-r002	Group:SED-MDX | rep:2	chromatograhy=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-3	nhilic-18may16-003-r001	Group:RUN-nNOS-/- | rep:1	chromatograhy=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-3	nhilic-18may16-003-r002	Group:RUN-nNOS-/- | rep:2	chromatograhy=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-4	nhilic-18may16-004-r001	Group:RUN-MDX | rep:1	chromatograhy=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-4	nhilic-18may16-004-r002	Group:RUN-MDX | rep:2	chromatograhy=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-5	nhilic-18may16-005-r001	Group:SED-MDX | rep:1	chromatograhy=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-5	nhilic-18may16-005-r002	Group:SED-MDX | rep:2	chromatograhy=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-6	nhilic-18may16-006-r001	Group:SED-BL10 Control | rep:1	chromatograhy=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-6	nhilic-18may16-006-r002	Group:SED-BL10 Control | rep:2	chromatograhy=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-7	nhilic-18may16-007-r001	Group:SED-MDX | rep:1	chromatograhy=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-7	nhilic-18may16-007-r002	Group:SED-MDX | rep:2	chromatograhy=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-8	nhilic-18may16-008-r001	Group:SED-nNOS-/- | rep:1	chromatograhy=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-8	nhilic-18may16-008-r002	Group:SED-nNOS-/- | rep:2	chromatograhy=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-9	nhilic-18may16-009-r001	Group:SED-BL10 Control | rep:1	chromatograhy=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-9	nhilic-18may16-009-r002	Group:SED-BL10 Control | rep:2	chromatograhy=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-10	nhilic-18may16-010-r001	Group:RUN-MDX | rep:1	chromatograhy=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-10	nhilic-18may16-010-r002	Group:RUN-MDX | rep:2	chromatograhy=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-11	nhilic-18may16-011-r001	Group:SED-BL10 Control | rep:1	chromatograhy=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-11	nhilic-18may16-011-r002	Group:SED-BL10 Control | rep:2	chromatograhy=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-12	nhilic-18may16-012-r001	Group:RUN-MDX | rep:1	chromatograhy=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-12	nhilic-18may16-012-r002	Group:RUN-MDX | rep:2	chromatograhy=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-13	nhilic-18may16-013-r001	Group:RUN-BL10 Control | rep:1	chromatograhy=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-13	nhilic-18may16-013-r002	Group:RUN-BL10 Control | rep:2	chromatograhy=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-14	nhilic-18may16-014-r001	Group:SED-nNOS-/- | rep:1	chromatograhy=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-14	nhilic-18may16-014-r002	Group:SED-nNOS-/- | rep:2	chromatograhy=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-15	nhilic-18may16-015-r001	Group:SED-BL10 Control | rep:1	chromatograhy=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-15	nhilic-18may16-015-r002	Group:SED-BL10 Control | rep:2	chromatograhy=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-16	nhilic-18may16-016-r001	Group:RUN-BL10 Control | rep:1	chromatograhy=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-16	nhilic-18may16-016-r002	Group:RUN-BL10 Control | rep:2	chromatograhy=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-17	nhilic-18may16-017-r001	Group:RUN-nNOS-/- | rep:1	chromatograhy=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-17	nhilic-18may16-017-r002	Group:RUN-nNOS-/- | rep:2	chromatograhy=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-18	nhilic-18may16-018-r001	Group:SED-MDX | rep:1	chromatograhy=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-18	nhilic-18may16-018-r002	Group:SED-MDX | rep:2	chromatograhy=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-19	nhilic-18may16-019-r001	Group:RUN-BL10 Control | rep:1	chromatograhy=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-19	nhilic-18may16-019-r002	Group:RUN-BL10 Control | rep:2	chromatograhy=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-20	nhilic-18may16-020-r001	Group:SED-nNOS-/- | rep:1	chromatograhy=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-20	nhilic-18may16-020-r002	Group:SED-nNOS-/- | rep:2	chromatograhy=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-21	nhilic-18may16-021-r001	Group:RUN-BL10 Control | rep:1	chromatograhy=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-21	nhilic-18may16-021-r002	Group:RUN-BL10 Control | rep:2	chromatograhy=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-22	nhilic-18may16-022-r001	Group:RUN-nNOS-/- | rep:1	chromatograhy=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-22	nhilic-18may16-022-r002	Group:RUN-nNOS-/- | rep:2	chromatograhy=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-23	nhilic-18may16-023-r001	Group:RUN-MDX | rep:1	chromatograhy=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-23	nhilic-18may16-023-r002	Group:RUN-MDX | rep:2	chromatograhy=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-24	nhilic-18may16-024-r001	Group:RUN-nNOS-/- | rep:1	chromatograhy=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-24	nhilic-18may16-024-r002	Group:RUN-nNOS-/- | rep:2	chromatograhy=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-25	nhilic-18may16-025-r001	Group:RUN-BL10 Control | rep:1	chromatograhy=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-25	nhilic-18may16-025-r002	Group:RUN-BL10 Control | rep:2	chromatograhy=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-26	nhilic-18may16-026-r001	Group:RUN-nNOS-/- | rep:1	chromatograhy=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-26	nhilic-18may16-026-r002	Group:RUN-nNOS-/- | rep:2	chromatograhy=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-27	nhilic-18may16-027-r001	Group:RUN-MDX | rep:1	chromatograhy=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-27	nhilic-18may16-027-r002	Group:RUN-MDX | rep:2	chromatograhy=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-28	nhilic-18may16-028-r001	Group:SED-nNOS-/- | rep:1	chromatograhy=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-28	nhilic-18may16-028-r002	Group:SED-nNOS-/- | rep:2	chromatograhy=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-29	nhilic-18may16-029-r001	Group:SED-MDX | rep:1	chromatograhy=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-29	nhilic-18may16-029-r002	Group:SED-MDX | rep:2	chromatograhy=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-30	nhilic-18may16-030-r001	Group:SED-BL10 Control | rep:1	chromatograhy=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-30	nhilic-18may16-030-r002	Group:SED-BL10 Control | rep:2	chromatograhy=NHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-1	philic-16may16-001-r001	Group:SED-nNOS-/- | rep:1	chromatograhy=PHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-1	philic-16may16-001-r002	Group:SED-nNOS-/- | rep:2	chromatograhy=PHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-2	philic-16may16-002-r001	Group:SED-MDX | rep:1	chromatograhy=PHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-2	philic-16may16-002-r002	Group:SED-MDX | rep:2	chromatograhy=PHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-3	philic-16may16-003-r001	Group:RUN-nNOS-/- | rep:1	chromatograhy=PHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-3	philic-16may16-003-r002	Group:RUN-nNOS-/- | rep:2	chromatograhy=PHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-4	philic-16may16-004-r001	Group:RUN-MDX | rep:1	chromatograhy=PHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-4	philic-16may16-004-r002	Group:RUN-MDX | rep:2	chromatograhy=PHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-5	philic-16may16-005-r001	Group:SED-MDX | rep:1	chromatograhy=PHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-5	philic-16may16-005-r002	Group:SED-MDX | rep:2	chromatograhy=PHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-6	philic-16may16-006-r001	Group:SED-BL10 Control | rep:1	chromatograhy=PHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-6	philic-16may16-006-r002	Group:SED-BL10 Control | rep:2	chromatograhy=PHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-7	philic-16may16-007-r001	Group:SED-MDX | rep:1	chromatograhy=PHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-7	philic-16may16-007-r002	Group:SED-MDX | rep:2	chromatograhy=PHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-8	philic-16may16-008-r001	Group:SED-nNOS-/- | rep:1	chromatograhy=PHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-8	philic-16may16-008-r002	Group:SED-nNOS-/- | rep:2	chromatograhy=PHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-9	philic-16may16-009-r001	Group:SED-BL10 Control | rep:1	chromatograhy=PHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-9	philic-16may16-009-r002	Group:SED-BL10 Control | rep:2	chromatograhy=PHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-10	philic-16may16-010-r001	Group:RUN-MDX | rep:1	chromatograhy=PHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-10	philic-16may16-010-r002	Group:RUN-MDX | rep:2	chromatograhy=PHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-11	philic-16may16-011-r001	Group:SED-BL10 Control | rep:1	chromatograhy=PHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-11	philic-16may16-011-r002	Group:SED-BL10 Control | rep:2	chromatograhy=PHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-12	philic-16may16-012-r001	Group:RUN-MDX | rep:1	chromatograhy=PHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-12	philic-16may16-012-r002	Group:RUN-MDX | rep:2	chromatograhy=PHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-13	philic-16may16-013-r001	Group:RUN-BL10 Control | rep:1	chromatograhy=PHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-13	philic-16may16-013-r002	Group:RUN-BL10 Control | rep:2	chromatograhy=PHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-14	philic-16may16-014-r001	Group:SED-nNOS-/- | rep:1	chromatograhy=PHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-14	philic-16may16-014-r002	Group:SED-nNOS-/- | rep:2	chromatograhy=PHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-15	philic-16may16-015-r001	Group:SED-BL10 Control | rep:1	chromatograhy=PHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-15	philic-16may16-015-r002	Group:SED-BL10 Control | rep:2	chromatograhy=PHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-16	philic-16may16-016-r001	Group:RUN-BL10 Control | rep:1	chromatograhy=PHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-16	philic-16may16-016-r002	Group:RUN-BL10 Control | rep:2	chromatograhy=PHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-17	philic-16may16-017-r001	Group:RUN-nNOS-/- | rep:1	chromatograhy=PHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-17	philic-16may16-017-r002	Group:RUN-nNOS-/- | rep:2	chromatograhy=PHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-18	philic-16may16-018-r001	Group:SED-MDX | rep:1	chromatograhy=PHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-18	philic-16may16-018-r002	Group:SED-MDX | rep:2	chromatograhy=PHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-19	philic-16may16-019-r001	Group:RUN-BL10 Control | rep:1	chromatograhy=PHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-19	philic-16may16-019-r002	Group:RUN-BL10 Control | rep:2	chromatograhy=PHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-20	philic-16may16-020-r001	Group:SED-nNOS-/- | rep:1	chromatograhy=PHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-20	philic-16may16-020-r002	Group:SED-nNOS-/- | rep:2	chromatograhy=PHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-21	philic-16may16-021-r001	Group:RUN-BL10 Control | rep:1	chromatograhy=PHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-21	philic-16may16-021-r002	Group:RUN-BL10 Control | rep:2	chromatograhy=PHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-22	philic-16may16-022-r001	Group:RUN-nNOS-/- | rep:1	chromatograhy=PHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-22	philic-16may16-022-r002	Group:RUN-nNOS-/- | rep:2	chromatograhy=PHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-23	philic-16may16-023-r001	Group:RUN-MDX | rep:1	chromatograhy=PHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-23	philic-16may16-023-r002	Group:RUN-MDX | rep:2	chromatograhy=PHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-24	philic-16may16-024-r001	Group:RUN-nNOS-/- | rep:1	chromatograhy=PHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-24	philic-16may16-024-r002	Group:RUN-nNOS-/- | rep:2	chromatograhy=PHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-25	philic-16may16-025-r001	Group:RUN-BL10 Control | rep:1	chromatograhy=PHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-25	philic-16may16-025-r002	Group:RUN-BL10 Control | rep:2	chromatograhy=PHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-26	philic-16may16-026-r001	Group:RUN-nNOS-/- | rep:1	chromatograhy=PHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-26	philic-16may16-026-r002	Group:RUN-nNOS-/- | rep:2	chromatograhy=PHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-27	philic-16may16-027-r001	Group:RUN-MDX | rep:1	chromatograhy=PHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-27	philic-16may16-027-r002	Group:RUN-MDX | rep:2	chromatograhy=PHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-28	philic-16may16-028-r001	Group:SED-nNOS-/- | rep:1	chromatograhy=PHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-28	philic-16may16-028-r002	Group:SED-nNOS-/- | rep:2	chromatograhy=PHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-29	philic-16may16-029-r001	Group:SED-MDX | rep:1	chromatograhy=PHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-29	philic-16may16-029-r002	Group:SED-MDX | rep:2	chromatograhy=PHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-30	philic-16may16-030-r001	Group:SED-BL10 Control | rep:1	chromatograhy=PHILIC
SUBJECT_SAMPLE_FACTORS           	ms5940-30	philic-16may16-030-r002	Group:SED-BL10 Control | rep:2	chromatograhy=PHILIC
#COLLECTION
CO:COLLECTION_SUMMARY            	Blood and Tissue Harvesting: Mice will be euthanized and blood will be taken by
CO:COLLECTION_SUMMARY            	cardiac puncture, centrifuged, and the plasma will be stored at -80ºC. The
CO:COLLECTION_SUMMARY            	heart, diaphragm, and muscles of both hindlimbs (gastrocnemius, soleus,
CO:COLLECTION_SUMMARY            	quadriceps) will be dissected and snap frozen in liquid nitrogen. Tibialis
CO:COLLECTION_SUMMARY            	anterior and plantaris muscles from both hindlimbs also will be dissected and
CO:COLLECTION_SUMMARY            	one set will be snap frozen in liquid nitrogen while the other set will be
CO:COLLECTION_SUMMARY            	mounted in OCT and frozen in isopentane cooled by liquid nitrogen. Cryosections
CO:COLLECTION_SUMMARY            	will be used to evaluate disease activity and exercise-induced muscle injury by
CO:COLLECTION_SUMMARY            	staining with: (a) hematoxylin and eosin to assess gross morphology, cellular
CO:COLLECTION_SUMMARY            	infiltration, and necrosis, (b) anti-F4/80 to label macrophages, and (c)
CO:COLLECTION_SUMMARY            	anti-IgG or IgM to label damaged muscle fibers.
#TREATMENT
TR:TREATMENT_SUMMARY             	"Studies will be performed in 12-16 week old male mdx mice, C57BL10 control
TR:TREATMENT_SUMMARY             	mice, and nNOS-/- mice obtained from Jackson Laboratory. All protocols will be
TR:TREATMENT_SUMMARY             	approved by the Penn State College of Medicine Institutional Animal Care and Use
TR:TREATMENT_SUMMARY             	Committee. Treadmill Exercise: Mice will run on a horizontal treadmill (Columbus
TR:TREATMENT_SUMMARY             	Instruments) to assess fatigue by a single bout of exercise beginning at 5 m/min
TR:TREATMENT_SUMMARY             	for 5 min followed by 1 m/min increases every minute until exhaustion. Electric
TR:TREATMENT_SUMMARY             	shocks will not be used to stimulate running due to adverse effects in mdx
TR:TREATMENT_SUMMARY             	mice."
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	mouse heart untarged metabolomics profiling
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_TYPE           	Reversed phase
CH:INSTRUMENT_NAME               	Agilent 1290 Infinity
CH:COLUMN_NAME                   	Waters Acquity HSS C18 (150 x 2.1mm, 1.8um)
#ANALYSIS
AN:ANALYSIS_TYPE                 	MS
#MS
MS:MS_COMMENTS                   	-
MS:INSTRUMENT_NAME               	Agilent 6550 QTOF
MS:INSTRUMENT_TYPE               	QTOF
MS:MS_TYPE                       	ESI
MS:ION_MODE                      	POSITIVE
MS:MS_RESULTS_FILE               	ST000653_AN000993_Results.txt	UNITS:intensity
#END