#METABOLOMICS WORKBENCH araskind_20170619_175520 DATATRACK_ID:1036 STUDY_ID:ST000734 ANALYSIS_ID:AN001148 PROJECT_ID:PR000531 VERSION 1 CREATED_ON July 3, 2017, 11:09 am #PROJECT PR:PROJECT_TITLE SCFA During Infection PR:PROJECT_TYPE MS analysis PR:PROJECT_SUMMARY Measuring SCFA following 24hr colonization by either C. difficile strain 630 or PR:PROJECT_SUMMARY strain VPI. Compared to uninfected mice. PR:INSTITUTE University of Michigan PR:DEPARTMENT Internal Medicine PR:LABORATORY Young Lab PR:LAST_NAME Young PR:FIRST_NAME Vincent PR:ADDRESS Ann Arbor, MI PR:EMAIL youngvi@med.umich.edu PR:PHONE 734-764-2237 #STUDY ST:STUDY_TITLE Measuring SCFA in mice gut following colonization by C. difficile ST:STUDY_TYPE MS analysis ST:STUDY_SUMMARY 3 cages of 5-8 week old C57B/6 mice were placed on the antibiotic cefoperazone ST:STUDY_SUMMARY for 10 days followed by 2 days of reovery on plain water. One cage remained on ST:STUDY_SUMMARY plain water the entire time. Of the three antibiotic treated cages. One was mock ST:STUDY_SUMMARY infected (uninfected control), one was infected with C. difficile strain VPI ST:STUDY_SUMMARY 10463, while the other was infected with C. difficile strain 630. Twenty-four ST:STUDY_SUMMARY hours after the infection the mice were eutanized and the ceal content was ST:STUDY_SUMMARY collected and snap frozen in liquid nitrogen. Samples were stored in the -80 ST:STUDY_SUMMARY until analysis. ST:INSTITUTE University of Michigan ST:DEPARTMENT Biomedical Research Core Facilities ST:LABORATORY Metabolomics core ST:LAST_NAME Kachman ST:FIRST_NAME Maureen ST:ADDRESS 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 ST:EMAIL mkachman@med.umich.edu ST:PHONE (734) 232-8175 ST:NUM_GROUPS 4 ST:TOTAL_SUBJECTS 4 #SUBJECT SU:SUBJECT_TYPE Mouse SU:SUBJECT_SPECIES Mus musculus SU:TAXONOMY_ID 10090 #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS SU0014790 S00022595 CEFOPERAZONE:NO | Infection:NO SUBJECT_SAMPLE_FACTORS SU0014790 S00022596 CEFOPERAZONE:NO | Infection:NO SUBJECT_SAMPLE_FACTORS SU0014790 S00022597 CEFOPERAZONE:NO | Infection:NO SUBJECT_SAMPLE_FACTORS SU0014791 S00022598 CEFOPERAZONE:YES | Infection:NO SUBJECT_SAMPLE_FACTORS SU0014791 S00022599 CEFOPERAZONE:YES | Infection:NO SUBJECT_SAMPLE_FACTORS SU0014791 S00022600 CEFOPERAZONE:YES | Infection:NO SUBJECT_SAMPLE_FACTORS SU0014791 S00022601 CEFOPERAZONE:YES | Infection:NO SUBJECT_SAMPLE_FACTORS SU0014791 S00022602 CEFOPERAZONE:YES | Infection:NO SUBJECT_SAMPLE_FACTORS SU0014792 S00022603 CEFOPERAZONE:YES | Infection:C.difficile VPI 10463 SUBJECT_SAMPLE_FACTORS SU0014792 S00022604 CEFOPERAZONE:YES | Infection:C.difficile VPI 10463 SUBJECT_SAMPLE_FACTORS SU0014792 S00022605 CEFOPERAZONE:YES | Infection:C.difficile VPI 10463 SUBJECT_SAMPLE_FACTORS SU0014792 S00022606 CEFOPERAZONE:YES | Infection:C.difficile VPI 10463 SUBJECT_SAMPLE_FACTORS SU0014792 S00022607 CEFOPERAZONE:YES | Infection:C.difficile VPI 10463 SUBJECT_SAMPLE_FACTORS SU0014793 S00022608 CEFOPERAZONE:YES | Infection:C.difficile 630 SUBJECT_SAMPLE_FACTORS SU0014793 S00022609 CEFOPERAZONE:YES | Infection:C.difficile 630 SUBJECT_SAMPLE_FACTORS SU0014793 S00022610 CEFOPERAZONE:YES | Infection:C.difficile 630 SUBJECT_SAMPLE_FACTORS SU0014793 S00022611 CEFOPERAZONE:YES | Infection:C.difficile 630 SUBJECT_SAMPLE_FACTORS SU0014793 S00022612 CEFOPERAZONE:YES | Infection:C.difficile 630 #COLLECTION CO:COLLECTION_SUMMARY - #TREATMENT TR:TREATMENT_SUMMARY - #SAMPLEPREP SP:SAMPLEPREP_SUMMARY - SP:SAMPLEPREP_PROTOCOL_FILENAME A007 - Short Chain Fatty Acids.pdf #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE GC CH:INSTRUMENT_NAME Agilent 7890N CH:COLUMN_NAME Phenomenex Zebron ZB-WAXplus (30m x 0.25mm, 0.25um) #ANALYSIS AN:ANALYSIS_TYPE MS AN:ANALYSIS_PROTOCOL_FILE A007 - Short Chain Fatty Acids.pdf #MS MS:MS_COMMENTS - MS:INSTRUMENT_NAME Agilent 5975 MS:INSTRUMENT_TYPE Single quadrupole MS:MS_TYPE EI MS:ION_MODE POSITIVE #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS nmol/mg MS_METABOLITE_DATA_START Samples S00022595 S00022596 S00022597 S00022598 S00022599 S00022600 S00022601 S00022602 S00022603 S00022604 S00022605 S00022606 S00022607 S00022608 S00022609 S00022610 S00022611 S00022612 Factors CEFOPERAZONE:NO | Infection:NO CEFOPERAZONE:NO | Infection:NO CEFOPERAZONE:NO | Infection:NO CEFOPERAZONE:YES | Infection:NO CEFOPERAZONE:YES | Infection:NO CEFOPERAZONE:YES | Infection:NO CEFOPERAZONE:YES | Infection:NO CEFOPERAZONE:YES | Infection:NO CEFOPERAZONE:YES | Infection:C.difficile VPI 10463 CEFOPERAZONE:YES | Infection:C.difficile VPI 10463 CEFOPERAZONE:YES | Infection:C.difficile VPI 10463 CEFOPERAZONE:YES | Infection:C.difficile VPI 10463 CEFOPERAZONE:YES | Infection:C.difficile VPI 10463 CEFOPERAZONE:YES | Infection:C.difficile 630 CEFOPERAZONE:YES | Infection:C.difficile 630 CEFOPERAZONE:YES | Infection:C.difficile 630 CEFOPERAZONE:YES | Infection:C.difficile 630 CEFOPERAZONE:YES | Infection:C.difficile 630 PROPIONIC ACID 5.840084378 5.025164743 5.728007939 4.664915791 5.003174825 2.074300723 6.533748413 5.290048349 .070904863 .045333804 .077458368 .071814321 .059701876 .053712994 .053209404 .038634593 .031298615 .04201874 ISOVALERIC ACID .089706861 .069187095 .084817708 .072504929 .068732594 .015811164 .06252755 .083709214 .010654133 .009235816 .014520628 .013444063 .012007614 .003276861 .009653162 .004418113 .004534621 .004965868 ACETIC ACID 25.59294197 25.41492366 28.4688173 2.866606396 9.63153058 4.594908649 7.921260485 3.878906437 1.479032999 .972716676 1.973832838 1.746918212 1.191550721 1.188376034 1.341207373 .806126607 .764126837 1.174697872 BUTYRIC ACID 8.61465711 11.65673252 12.05432854 1.923871524 3.514100872 .493128939 1.179575308 1.868520115 .180448284 .057834738 .153882618 .128358278 .11396217 .149965956 .076179454 .055860094 .039527266 .093361116 OCTANOIC ACID .025932249 .010373652 .012243175 .009419153 .0126574 .011599976 .008438677 .01961425 .005805574 .005689784 .007483888 .007176709 .00638579 .010068842 .008814481 .008850281 .012685614 .012274529 VALERIC ACID .432063293 .44141974 .504528232 .061734397 .288796793 .015504573 .021711761 .047579943 .012914933 .005975966 .012380643 .009758525 .010318437 .027223253 .008541028 .007159443 .00445641 .015370977 HEPTANOIC ACID .005741537 .002880174 .002584118 .001060799 .00103679 .00144478 .001079936 .000378714 .000863618 .000221175 .000322775 .000258454 .000538541 .00149324 .000292595 .000379465 .000354646 .000674351 HEXANOIC ACID .018829258 .011292956 .011096831 .004118441 .006877033 .004368695 .003672636 .003144908 .00137236 .001552075 .001409975 .001526006 .002077386 .003169532 .002258753 .002408635 .001950788 .004657346 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name moverz_quant ri ri_type pubchem_id inchi_key kegg_id other_id other_id_type PROPIONIC ACID 1032 ISOVALERIC ACID 10430 ACETIC ACID 176 BUTYRIC ACID 264 OCTANOIC ACID 379 VALERIC ACID 7991 HEPTANOIC ACID 8094 HEXANOIC ACID 8892 METABOLITES_END #END