#METABOLOMICS WORKBENCH xujiuliang_20170704_000218 DATATRACK_ID:1109 STUDY_ID:ST000766 ANALYSIS_ID:AN001212 PROJECT_ID:PR000552 VERSION 1 CREATED_ON July 5, 2017, 9:57 am #PROJECT PR:PROJECT_TITLE Soybean project PR:PROJECT_SUMMARY Non-targeted metabolic profiling of 26 soybean varieties using liquid PR:PROJECT_SUMMARY chromatography-mass spectrometry (LC-MS) including 15 wild black soybeans (WBS) PR:PROJECT_SUMMARY and 11 cultivated black soybeans (CBS), combined with multivariate analysis, PR:PROJECT_SUMMARY revealed significant differences in25 differential metabolites PR:INSTITUTE KIST PR:LAST_NAME Xu PR:FIRST_NAME Jiuliang PR:ADDRESS saimdang-ro 679, Gangneung, Gangwon, 213510, Korea, South PR:EMAIL 213510@kist.re.kr PR:PHONE 01024398802 #STUDY ST:STUDY_TITLE Differences in metabolite profiles and antioxidant activities between wild and ST:STUDY_TITLE cultivated black soybeans evaluated by a correlation analysis ST:STUDY_SUMMARY Non-targeted metabolic profiling of 26 soybean varieties using liquid ST:STUDY_SUMMARY chromatography-mass spectrometry (LC-MS) including 15 wild black soybeans (WBS) ST:STUDY_SUMMARY and 11 cultivated black soybeans (CBS), combined with multivariate analysis, ST:STUDY_SUMMARY revealed significant differences in 25 differential metabolites ST:INSTITUTE KIST ST:LAST_NAME Xu ST:FIRST_NAME Jiuliang ST:ADDRESS saimdang-ro 679, Gangneung, Gangwon, 213510, Korea, South ST:EMAIL 213510@kist.re.kr ST:PHONE 01024398802 #SUBJECT SU:SUBJECT_TYPE plant SU:SUBJECT_SPECIES Glycine max SU:TAXONOMY_ID 3847 #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS - 01_CBS_1 type:Cultivar SUBJECT_SAMPLE_FACTORS - 02_CBS_2 type:Cultivar SUBJECT_SAMPLE_FACTORS - 03_CBS_3 type:Cultivar SUBJECT_SAMPLE_FACTORS - 04_CBS_4 type:Cultivar SUBJECT_SAMPLE_FACTORS - 05_CBS_5 type:Cultivar SUBJECT_SAMPLE_FACTORS - 06_CBS_6 type:Cultivar SUBJECT_SAMPLE_FACTORS - 07_CBS_7 type:Cultivar SUBJECT_SAMPLE_FACTORS - 11_CBS_8 type:Cultivar SUBJECT_SAMPLE_FACTORS - 12_CBS_9 type:Cultivar SUBJECT_SAMPLE_FACTORS - 13_CBS_10 type:Cultivar SUBJECT_SAMPLE_FACTORS - 14_CBS_11 type:Cultivar SUBJECT_SAMPLE_FACTORS - 08_CBS_QC1 type:Cultivar SUBJECT_SAMPLE_FACTORS - 18_CBS_QC2 type:Cultivar SUBJECT_SAMPLE_FACTORS - 28_CBS_QC3 type:Cultivar SUBJECT_SAMPLE_FACTORS - 15_WBS_1 type:Wild SUBJECT_SAMPLE_FACTORS - 16_WBS_2 type:Wild SUBJECT_SAMPLE_FACTORS - 17_WBS_3 type:Wild SUBJECT_SAMPLE_FACTORS - 21_WBS_4 type:Wild SUBJECT_SAMPLE_FACTORS - 22_WBS_5 type:Wild SUBJECT_SAMPLE_FACTORS - 23_WBS_6 type:Wild SUBJECT_SAMPLE_FACTORS - 24_WBS_7 type:Wild SUBJECT_SAMPLE_FACTORS - 25_WBS_8 type:Wild SUBJECT_SAMPLE_FACTORS - 26_WBS_9 type:Wild SUBJECT_SAMPLE_FACTORS - 27_WBS_10 type:Wild SUBJECT_SAMPLE_FACTORS - 31_WBS_11 type:Wild SUBJECT_SAMPLE_FACTORS - 32_WBS_12 type:Wild SUBJECT_SAMPLE_FACTORS - 33_WBS_13 type:Wild SUBJECT_SAMPLE_FACTORS - 34_WBS_14 type:Wild SUBJECT_SAMPLE_FACTORS - 35_WBS_15 type:Wild SUBJECT_SAMPLE_FACTORS - 09_WBS_QC1 type:Wild SUBJECT_SAMPLE_FACTORS - 19_WBS_QC2 type:Wild SUBJECT_SAMPLE_FACTORS - 29_WBS_QC3 type:Wild #COLLECTION CO:COLLECTION_SUMMARY Fifteen wild and 11 cultivated black soybeans were selected from the soybean CO:COLLECTION_SUMMARY germplasm collections that have been maintained by the National Institute of CO:COLLECTION_SUMMARY Crop Science in Korea #TREATMENT TR:TREATMENT_SUMMARY The planting density was 15 cm between plants in a row, and the rows were 70 cm TR:TREATMENT_SUMMARY apart. Field management, including irrigation, fertilizer application, and pest TR:TREATMENT_SUMMARY control followed essentially normal agricultural practices according to Rural TR:TREATMENT_SUMMARY Development Administration standard protocol #SAMPLEPREP SP:SAMPLEPREP_SUMMARY Dried black soybeans were ground into a fine powder and sieved through a screen SP:SAMPLEPREP_SUMMARY with a 55 mesh count, then 40 mg of the powder was mixed with 70% aqueous SP:SAMPLEPREP_SUMMARY methanol (4mL, 1% HCl) containing 1.5 μg/ml phlorizin as an internal standard SP:SAMPLEPREP_SUMMARY and sonicated for 30 min. The mixture was kept for approximately 16 h at room SP:SAMPLEPREP_SUMMARY temperature in the dark.vacuum.Supernatants were filtered through a 0.20-μm SP:SAMPLEPREP_SUMMARY syringe filter and freeze dried then reconstituted with 0.5 ml of 50% aqueous SP:SAMPLEPREP_SUMMARY methanol for LC-MS analysis. #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE Reversed phase CH:INSTRUMENT_NAME Agilent 1290 CH:COLUMN_NAME Prevail C18 #ANALYSIS AN:ANALYSIS_TYPE MS #MS MS:MS_COMMENTS - MS:INSTRUMENT_NAME Bruker Daltonics MS:INSTRUMENT_TYPE QTOF MS:MS_TYPE ESI MS:ION_MODE POSITIVE MS:MS_RESULTS_FILE ST000766_AN001212_Results.txt UNITS:peal area #END