#METABOLOMICS WORKBENCH araskind_20170802_183923 DATATRACK_ID:1184 STUDY_ID:ST000834 ANALYSIS_ID:AN001335 STUDY_ID:ST000834 ANALYSIS_ID:AN001425 PROJECT_ID:PR000595 VERSION 1 CREATED_ON October 3, 2017, 9:37 am #PROJECT PR:PROJECT_TITLE Purine and TCA Measurements in SS Hypertension PR:PROJECT_TYPE MS analysis PR:PROJECT_SUMMARY Based on RNA-seq ontological and Bayesian analyses, both purine levels and PR:PROJECT_SUMMARY TCA-cycle intermediates have been nominated as being regulated by pressure in PR:PROJECT_SUMMARY the SS rat. This project seeks to verify the previous RNA-seq prediction. PR:INSTITUTE Medical College of Wisconsin PR:DEPARTMENT Physiology PR:LABORATORY Cowley Lab PR:LAST_NAME Cowley PR:FIRST_NAME Allen PR:ADDRESS Milwaukee, WI PR:EMAIL cowley@mcw.edu PR:PHONE 414-955-8277 #STUDY ST:STUDY_TITLE Purine and TCA measurements in salt sensitive (SS) hypertension ST:STUDY_TYPE MS analysis ST:STUDY_SUMMARY SS rats were surgically implanted with a chronic servo-control cuff, the purpose ST:STUDY_SUMMARY of which is to maintain normal pressure to the left kidney while the right ST:STUDY_SUMMARY kidney is exposed to high blood pressure. After 7 days of high salt treatment, ST:STUDY_SUMMARY which induces high blood pressure in the SS rat, rats were anesthetized with ST:STUDY_SUMMARY pentobarbital, kidneys were flushed and removed. The renal medulla was separated ST:STUDY_SUMMARY from the cortex using scissors, and the renal medullas were frozen in liquid ST:STUDY_SUMMARY nitrogen and stored at -80 C. ST:INSTITUTE University of Michigan ST:DEPARTMENT Biomedical Research Core Facilities ST:LABORATORY Metabolomics core ST:LAST_NAME Kachman ST:FIRST_NAME Maureen ST:ADDRESS 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 ST:EMAIL mkachman@med.umich.edu ST:PHONE (734) 232-8175 ST:NUM_GROUPS 11 ST:TOTAL_SUBJECTS 4 #SUBJECT SU:SUBJECT_TYPE RAT SU:SUBJECT_SPECIES Rattus norvegicus SU:TAXONOMY_ID 10114 #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS SU0016139 S00025608 Side:Left BP (mmHg)=127.0; Rat=1 SUBJECT_SAMPLE_FACTORS SU0016139 S00025609 Side:Right BP (mmHg)=147.0; Rat=1 SUBJECT_SAMPLE_FACTORS SU0016141 S00025610 Side:Left BP (mmHg)=128.0; Rat=2 SUBJECT_SAMPLE_FACTORS SU0016141 S00025611 Side:Right BP (mmHg)=147.0; Rat=2 SUBJECT_SAMPLE_FACTORS SU0016143 S00025612 Side:Left BP (mmHg)=124.0; Rat=3 SUBJECT_SAMPLE_FACTORS SU0016143 S00025613 Side:Right BP (mmHg)=149.0; Rat=3 SUBJECT_SAMPLE_FACTORS SU0016145 S00025614 Side:Left BP (mmHg)=n/a; Rat=n/a SUBJECT_SAMPLE_FACTORS SU0016145 S00025615 Side:Right BP (mmHg)=n/a; Rat=n/a #COLLECTION CO:COLLECTION_SUMMARY - #TREATMENT TR:TREATMENT_SUMMARY - #SAMPLEPREP SP:SAMPLEPREP_SUMMARY - SP:SAMPLEPREP_PROTOCOL_FILENAME A011_Glycolysis-TCA-nucleotides.pdf #CHROMATOGRAPHY CH:CHROMATOGRAPHY_SUMMARY 5% Phenyl Methyl Siloxane CH:CHROMATOGRAPHY_TYPE GC CH:INSTRUMENT_NAME Agilent 7890N CH:COLUMN_NAME Agilent HP5-MS (15m × 0.25mm, 0.25 um) #ANALYSIS AN:ANALYSIS_TYPE MS AN:ANALYSIS_PROTOCOL_FILE A011 - Glycolysis-TCA-nucleotides.pdf AN:ACQUISITION_PARAMETERS_FILE HEIDI_FAMES_10MIN.M.zip #MS MS:MS_COMMENTS - MS:INSTRUMENT_NAME Agilent 5975 MS:INSTRUMENT_TYPE Single quadrupole MS:MS_TYPE EI MS:ION_MODE POSITIVE #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS nmol/mg MS_METABOLITE_DATA_START Samples S00025608 S00025609 S00025610 S00025611 S00025612 S00025613 S00025614 S00025615 Factors Side:Left Side:Right Side:Left Side:Right Side:Left Side:Right Side:Left Side:Right PHOSPHOENOLPYRUVATE 0.002238075 0.002936561 0.001497128 0.001079579 0.001768693 D-ERYTHROSE 4-PHOSPHATE 0.014010332 0.016984821 0.01526438 0.052889418 0.036581065 0.000853245 BETA-NAD+ 0.06374692 0.0700109 0.054146377 0.067540817 0.08577401 0.133515745 0.09906959 0.091508614 COENZYME II 0.009813115 0.009884822 0.014766304 0.0121121 0.012978596 0.007816356 0.008386053 0.012208726 SEDOHEPTULOSE 7-PHOSPHATE 0.083986073 0.077729168 0.061124042 0.098403809 0.10426337 0.070846085 0.072724669 0.087073947 D-FRUCTOSE, 1,6-BIS(DIHYDROGEN PHOSPHATE) 0.013320433 0.014285476 0.016721931 0.014346555 0.019849205 0.018468204 0.012829552 0.017093124 NADP-REDUCED 0.008387778 0.008428906 0.007512053 0.009540162 0.011727217 0.00976995 0.007538922 0.008385149 CITRIC ACID 0.030662316 0.022396995 0.011537887 0.021981468 0.012954692 0.033076688 0.017976974 0.013836711 NADH 0.017926504 0.022909499 0.024443716 0.018134525 0.018851791 MALIC ACID 0.022499058 0.021519623 0.080753346 0.027962912 0.026168585 0.023875585 0.017976334 0.040948187 ADENOSINE TRIPHOSPHATE 0.152075649 0.287947471 0.061864497 0.24955847 0.230327265 0.163777487 0.143692973 0.178190663 SUCROSE 0.010345131 0.012354612 0.022875568 0.014202025 0.01962373 0.013422085 0.008699082 0.022132336 ADENOSINE 5'-DIPHOSPHATE 0.089739374 0.096910332 0.04465881 0.09643351 0.075164528 0.053531037 0.060481792 0.063066025 GLYCERATE 3-PHOSPHATE 0.159103562 0.175000564 0.152744093 0.202390679 0.296113929 2.481637296 0.317153825 0.295905343 RIBOSE PHOSPHATE 0.02365829 0.027799435 0.030244893 0.026928424 0.040660352 0.05223231 0.026300231 0.033856338 6-PHOSPHOGLUCONIC ACID 0.009685428 0.011277527 0.013712325 0.010531027 0.016499074 0.009863179 0.009594665 0.01428308 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name moverz_quant ri ri_type pubchem_id inchi_key kegg_id other_id other_id_type PHOSPHOENOLPYRUVATE 1005 D-ERYTHROSE 4-PHOSPHATE 122357 BETA-NAD+ 15938971 COENZYME II 15938972 SEDOHEPTULOSE 7-PHOSPHATE 165007 D-FRUCTOSE, 1,6-BIS(DIHYDROGEN PHOSPHATE) 21125049 NADP-REDUCED 22833512 CITRIC ACID 311 NADH 439153 MALIC ACID 525 ADENOSINE TRIPHOSPHATE 5957 SUCROSE 5988 ADENOSINE 5'-DIPHOSPHATE 6022 GLYCERATE 3-PHOSPHATE 724 RIBOSE PHOSPHATE 77982 6-PHOSPHOGLUCONIC ACID 91493 METABOLITES_END #END