#METABOLOMICS WORKBENCH hormel101_20180409_155755 DATATRACK_ID:1361 STUDY_ID:ST000948 ANALYSIS_ID:AN001556 PROJECT_ID:PR000652 VERSION 1 CREATED_ON April 10, 2018, 3:05 pm #PROJECT PR:PROJECT_TITLE Mayo Pilot and Feasibility: Mechanisms for Insulin Resistance in Polycystic PR:PROJECT_TITLE Ovary Syndrome PR:PROJECT_SUMMARY Polycystic Ovary Syndrome (PCOS), a condition of androgen excess, infrequent PR:PROJECT_SUMMARY ovulation, and insulin resistance is the most common endocrine disorder among PR:PROJECT_SUMMARY premenopausal women. Little is known about the exact mechanisms of insulin PR:PROJECT_SUMMARY resistance in PCOS and how metformin can improve insulin sensitivity, increase PR:PROJECT_SUMMARY the frequency of ovulation and lower androgens in PCOS. Preliminary data from PR:PROJECT_SUMMARY metabolomic analyses of amino acids demonstrate increased concentrations of PR:PROJECT_SUMMARY lysine and its metabolite, α-aminoadipic acid (AAA), in PCOS versus obese PR:PROJECT_SUMMARY controls. Interestingly, greater AAA concentrations predicted the development of PR:PROJECT_SUMMARY type 2 diabetes in the Framingham epidemiologic cohort, experimentally lowers PR:PROJECT_SUMMARY glucose in animal models and increases insulin secretion in vitro. To date, the PR:PROJECT_SUMMARY mechanism for increased circulating concentrations of lysine and AAA in PR:PROJECT_SUMMARY insulin-resistant individuals is not known. Building upon these findings, we PR:PROJECT_SUMMARY have initiated a project to simultaneously study lysine and AAA kinetics for the PR:PROJECT_SUMMARY first time in insulin-resistant individuals using stable isotope tracer PR:PROJECT_SUMMARY methodology. We will evaluate: 1) whether lysine and AAA kinetics are altered in PR:PROJECT_SUMMARY PCOS versus healthy controls; 2) the effect of hyperinsulinemia on lysine and PR:PROJECT_SUMMARY AAA kinetics in PCOS versus controls; 3) whether treatment to improve insulin PR:PROJECT_SUMMARY sensitivity changes lysine and AAA kinetics in PCOS. The long-term goal is to PR:PROJECT_SUMMARY target pathways for the treatment of PCOS and the prevention of type 2 diabetes PR:PROJECT_SUMMARY in PCOS and other insulin-resistant individuals at greater risk for type 2 PR:PROJECT_SUMMARY diabetes. PR:INSTITUTE Mayo Clinic PR:LAST_NAME Chang PR:FIRST_NAME Alice PR:ADDRESS 200 First St. SW, Rochester, Minnesota, 55905, USA PR:EMAIL Chang.Alice1@mayo.edu PR:PHONE 507-286-0505 #STUDY ST:STUDY_TITLE Mechanisms for Insulin Resistance in Polycystic Ovary Syndrome: aminoadipic ST:STUDY_TITLE acid, lysine concentrations, and enrichment (part II) ST:STUDY_SUMMARY "To determine how metformin therapy changes lysine and AAA kinetics in PCOS and ST:STUDY_SUMMARY whether this is associated with improvements in insulin sensitivity. Changes in ST:STUDY_SUMMARY lysine and AAA flux before and after three months of metformin therapy will be ST:STUDY_SUMMARY compared to women with PCOS randomized to no treatment for three months. ST:INSTITUTE Mayo Clinic ST:LAST_NAME Chang ST:FIRST_NAME Alice ST:ADDRESS 200 First St. SW, Rochester, Minnesota, 55905, USA ST:EMAIL Chang.Alice1@mayo.edu ST:PHONE 507-286-0505 #SUBJECT SU:SUBJECT_TYPE Human SU:SUBJECT_SPECIES Homo sapiens SU:TAXONOMY_ID 9606 #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS 9980 ms6436-12 Time point:baseline | group:PCOS visit=1; Date of study=5/12/2016 SUBJECT_SAMPLE_FACTORS 9980 ms6436-13 Time point:-60 | group:PCOS visit=1; Date of study=5/13/2016 SUBJECT_SAMPLE_FACTORS 9980 ms6436-14 Time point:-45 | group:PCOS visit=1; Date of study=5/13/2016 SUBJECT_SAMPLE_FACTORS 9980 ms6436-15 Time point:-30 | group:PCOS visit=1; Date of study=5/13/2016 SUBJECT_SAMPLE_FACTORS 9980 ms6436-16 Time point:-15 | group:PCOS visit=1; Date of study=5/13/2016 SUBJECT_SAMPLE_FACTORS 9980 ms6436-17 Time point:60 | group:PCOS visit=1; Date of study=5/13/2016 SUBJECT_SAMPLE_FACTORS 9980 ms6436-18 Time point:120 | group:PCOS visit=1; Date of study=5/13/2016 SUBJECT_SAMPLE_FACTORS 9980 ms6436-19 Time point:135 | group:PCOS visit=1; Date of study=5/13/2016 SUBJECT_SAMPLE_FACTORS 9980 ms6436-20 Time point:150 | group:PCOS visit=1; Date of study=5/13/2016 SUBJECT_SAMPLE_FACTORS 9980 ms6436-21 Time point:165 | group:PCOS visit=1; Date of study=5/13/2016 SUBJECT_SAMPLE_FACTORS 9980 ms6436-22 Time point:180 | group:PCOS visit=1; Date of study=5/13/2016 SUBJECT_SAMPLE_FACTORS 2218 ms6437-12 Time point:baseline | group:PCOS visit=1; Date of study=5/9/2016 SUBJECT_SAMPLE_FACTORS 2218 ms6437-13 Time point:-60 | group:PCOS visit=1; Date of study=5/10/2016 SUBJECT_SAMPLE_FACTORS 2218 ms6437-14 Time point:-45 | group:PCOS visit=1; Date of study=5/10/2016 SUBJECT_SAMPLE_FACTORS 2218 ms6437-15 Time point:-30 | group:PCOS visit=1; Date of study=5/10/2016 SUBJECT_SAMPLE_FACTORS 2218 ms6437-16 Time point:-15 | group:PCOS visit=1; Date of study=5/10/2016 SUBJECT_SAMPLE_FACTORS 2218 ms6437-17 Time point:60 | group:PCOS visit=1; Date of study=5/10/2016 SUBJECT_SAMPLE_FACTORS 2218 ms6437-18 Time point:120 | group:PCOS visit=1; Date of study=5/10/2016 SUBJECT_SAMPLE_FACTORS 2218 ms6437-19 Time point:135 | group:PCOS visit=1; Date of study=5/10/2016 SUBJECT_SAMPLE_FACTORS 2218 ms6437-20 Time point:150 | group:PCOS visit=1; Date of study=5/10/2016 SUBJECT_SAMPLE_FACTORS 2218 ms6437-21 Time point:165 | group:PCOS visit=1; Date of study=5/10/2016 SUBJECT_SAMPLE_FACTORS 2218 ms6437-22 Time point:180 | group:PCOS visit=1; Date of study=5/10/2016 SUBJECT_SAMPLE_FACTORS 1228 ms6438-12 Time point:baseline | group:PCOS visit=1; Date of study=4/5/2016 SUBJECT_SAMPLE_FACTORS 1228 ms6438-13 Time point:-60 | group:PCOS visit=1; Date of study=4/6/2016 SUBJECT_SAMPLE_FACTORS 1228 ms6438-14 Time point:-45 | group:PCOS visit=1; Date of study=4/6/2016 SUBJECT_SAMPLE_FACTORS 1228 ms6438-15 Time point:-30 | group:PCOS visit=1; Date of study=4/6/2016 SUBJECT_SAMPLE_FACTORS 1228 ms6438-16 Time point:-15 | group:PCOS visit=1; Date of study=4/6/2016 SUBJECT_SAMPLE_FACTORS 1228 ms6438-17 Time point:60 | group:PCOS visit=1; Date of study=4/6/2016 SUBJECT_SAMPLE_FACTORS 1228 ms6438-18 Time point:120 | group:PCOS visit=1; Date of study=4/6/2016 SUBJECT_SAMPLE_FACTORS 1228 ms6438-19 Time point:135 | group:PCOS visit=1; Date of study=4/6/2016 SUBJECT_SAMPLE_FACTORS 1228 ms6438-20 Time point:150 | group:PCOS visit=1; Date of study=4/6/2016 SUBJECT_SAMPLE_FACTORS 1228 ms6438-21 Time point:165 | group:PCOS visit=1; Date of study=4/6/2016 SUBJECT_SAMPLE_FACTORS 1228 ms6438-22 Time point:180 | group:PCOS visit=1; Date of study=4/6/2016 SUBJECT_SAMPLE_FACTORS 4434 ms6439-12 Time point:baseline | group:PCOS visit=1; Date of study=5/4/2016 SUBJECT_SAMPLE_FACTORS 4434 ms6439-13 Time point:-60 | group:PCOS visit=1; Date of study=5/5/2016 SUBJECT_SAMPLE_FACTORS 4434 ms6439-14 Time point:-45 | group:PCOS visit=1; Date of study=5/5/2016 SUBJECT_SAMPLE_FACTORS 4434 ms6439-15 Time point:-30 | group:PCOS visit=1; Date of study=5/5/2016 SUBJECT_SAMPLE_FACTORS 4434 ms6439-16 Time point:-15 | group:PCOS visit=1; Date of study=5/5/2016 SUBJECT_SAMPLE_FACTORS 4434 ms6439-17 Time point:60 | group:PCOS visit=1; Date of study=5/5/2016 SUBJECT_SAMPLE_FACTORS 4434 ms6439-18 Time point:120 | group:PCOS visit=1; Date of study=5/5/2016 SUBJECT_SAMPLE_FACTORS 4434 ms6439-19 Time point:135 | group:PCOS visit=1; Date of study=5/5/2016 SUBJECT_SAMPLE_FACTORS 4434 ms6439-20 Time point:150 | group:PCOS visit=1; Date of study=5/5/2016 SUBJECT_SAMPLE_FACTORS 4434 ms6439-21 Time point:165 | group:PCOS visit=1; Date of study=5/5/2016 SUBJECT_SAMPLE_FACTORS 4434 ms6439-22 Time point:180 | group:PCOS visit=1; Date of study=5/5/2016 SUBJECT_SAMPLE_FACTORS 9980 ms6436-1 Time point:baseline | group:PCOS visit=2; Date of study=9/8/2016 SUBJECT_SAMPLE_FACTORS 9980 ms6436-2 Time point:-60 | group:PCOS visit=2; Date of study=9/8/2016 SUBJECT_SAMPLE_FACTORS 9980 ms6436-3 Time point:-45 | group:PCOS visit=2; Date of study=9/8/2016 SUBJECT_SAMPLE_FACTORS 9980 ms6436-4 Time point:-30 | group:PCOS visit=2; Date of study=9/8/2016 SUBJECT_SAMPLE_FACTORS 9980 ms6436-5 Time point:-15 | group:PCOS visit=2; Date of study=9/8/2016 SUBJECT_SAMPLE_FACTORS 9980 ms6436-6 Time point:60 | group:PCOS visit=2; Date of study=9/8/2016 SUBJECT_SAMPLE_FACTORS 9980 ms6436-7 Time point:120 | group:PCOS visit=2; Date of study=9/8/2016 SUBJECT_SAMPLE_FACTORS 9980 ms6436-8 Time point:135 | group:PCOS visit=2; Date of study=9/8/2016 SUBJECT_SAMPLE_FACTORS 9980 ms6436-9 Time point:150 | group:PCOS visit=2; Date of study=9/8/2016 SUBJECT_SAMPLE_FACTORS 9980 ms6436-10 Time point:165 | group:PCOS visit=2; Date of study=9/8/2016 SUBJECT_SAMPLE_FACTORS 9980 ms6436-11 Time point:180 | group:PCOS visit=2; Date of study=9/8/2016 SUBJECT_SAMPLE_FACTORS 2218 ms6437-1 Time point:baseline | group:PCOS visit=2; Date of study=8/22/2016 SUBJECT_SAMPLE_FACTORS 2218 ms6437-2 Time point:-60 | group:PCOS visit=2; Date of study=8/22/2016 SUBJECT_SAMPLE_FACTORS 2218 ms6437-3 Time point:-45 | group:PCOS visit=2; Date of study=8/22/2016 SUBJECT_SAMPLE_FACTORS 2218 ms6437-4 Time point:-30 | group:PCOS visit=2; Date of study=8/22/2016 SUBJECT_SAMPLE_FACTORS 2218 ms6437-5 Time point:-15 | group:PCOS visit=2; Date of study=8/22/2016 SUBJECT_SAMPLE_FACTORS 2218 ms6437-6 Time point:60 | group:PCOS visit=2; Date of study=8/22/2016 SUBJECT_SAMPLE_FACTORS 2218 ms6437-7 Time point:120 | group:PCOS visit=2; Date of study=8/22/2016 SUBJECT_SAMPLE_FACTORS 2218 ms6437-8 Time point:135 | group:PCOS visit=2; Date of study=8/22/2016 SUBJECT_SAMPLE_FACTORS 2218 ms6437-9 Time point:150 | group:PCOS visit=2; Date of study=8/22/2016 SUBJECT_SAMPLE_FACTORS 2218 ms6437-10 Time point:165 | group:PCOS visit=2; Date of study=8/22/2016 SUBJECT_SAMPLE_FACTORS 2218 ms6437-11 Time point:180 | group:PCOS visit=2; Date of study=8/22/2016 SUBJECT_SAMPLE_FACTORS 1228 ms6438-1 Time point:baseline | group:PCOS visit=2; Date of study=7/13/2016 SUBJECT_SAMPLE_FACTORS 1228 ms6438-2 Time point:-60 | group:PCOS visit=2; Date of study=7/13/2016 SUBJECT_SAMPLE_FACTORS 1228 ms6438-3 Time point:-45 | group:PCOS visit=2; Date of study=7/13/2016 SUBJECT_SAMPLE_FACTORS 1228 ms6438-4 Time point:-30 | group:PCOS visit=2; Date of study=7/13/2016 SUBJECT_SAMPLE_FACTORS 1228 ms6438-5 Time point:-15 | group:PCOS visit=2; Date of study=7/13/2016 SUBJECT_SAMPLE_FACTORS 1228 ms6438-6 Time point:60 | group:PCOS visit=2; Date of study=7/13/2016 SUBJECT_SAMPLE_FACTORS 1228 ms6438-7 Time point:120 | group:PCOS visit=2; Date of study=7/13/2016 SUBJECT_SAMPLE_FACTORS 1228 ms6438-8 Time point:135 | group:PCOS visit=2; Date of study=7/13/2016 SUBJECT_SAMPLE_FACTORS 1228 ms6438-9 Time point:150 | group:PCOS visit=2; Date of study=7/13/2016 SUBJECT_SAMPLE_FACTORS 1228 ms6438-10 Time point:165 | group:PCOS visit=2; Date of study=7/13/2016 SUBJECT_SAMPLE_FACTORS 1228 ms6438-11 Time point:180 | group:PCOS visit=2; Date of study=7/13/2016 SUBJECT_SAMPLE_FACTORS 4434 ms6439-1 Time point:baseline | group:PCOS visit=2; Date of study=8/4/2016 SUBJECT_SAMPLE_FACTORS 4434 ms6439-2 Time point:-60 | group:PCOS visit=2; Date of study=8/4/2016 SUBJECT_SAMPLE_FACTORS 4434 ms6439-3 Time point:-45 | group:PCOS visit=2; Date of study=8/4/2016 SUBJECT_SAMPLE_FACTORS 4434 ms6439-4 Time point:-30 | group:PCOS visit=2; Date of study=8/4/2016 SUBJECT_SAMPLE_FACTORS 4434 ms6439-5 Time point:-15 | group:PCOS visit=2; Date of study=8/4/2016 SUBJECT_SAMPLE_FACTORS 4434 ms6439-6 Time point:60 | group:PCOS visit=2; Date of study=8/4/2016 SUBJECT_SAMPLE_FACTORS 4434 ms6439-7 Time point:120 | group:PCOS visit=2; Date of study=8/4/2016 SUBJECT_SAMPLE_FACTORS 4434 ms6439-8 Time point:135 | group:PCOS visit=2; Date of study=8/4/2016 SUBJECT_SAMPLE_FACTORS 4434 ms6439-9 Time point:150 | group:PCOS visit=2; Date of study=8/4/2016 SUBJECT_SAMPLE_FACTORS 4434 ms6439-10 Time point:165 | group:PCOS visit=2; Date of study=8/4/2016 SUBJECT_SAMPLE_FACTORS 4434 ms6439-11 Time point:180 | group:PCOS visit=2; Date of study=8/4/2016 SUBJECT_SAMPLE_FACTORS 5908 ms6440-12 Time point:baseline | group:PCOS visit=1; Date of study=5/3/2016 SUBJECT_SAMPLE_FACTORS 5908 ms6440-13 Time point:-60 | group:PCOS visit=1; Date of study=5/4/2016 SUBJECT_SAMPLE_FACTORS 5908 ms6440-14 Time point:-45 | group:PCOS visit=1; Date of study=5/4/2016 SUBJECT_SAMPLE_FACTORS 5908 ms6440-15 Time point:-30 | group:PCOS visit=1; Date of study=5/4/2016 SUBJECT_SAMPLE_FACTORS 5908 ms6440-16 Time point:-15 | group:PCOS visit=1; Date of study=5/4/2016 SUBJECT_SAMPLE_FACTORS 5908 ms6440-17 Time point:60 | group:PCOS visit=1; Date of study=5/4/2016 SUBJECT_SAMPLE_FACTORS 5908 ms6440-18 Time point:120 | group:PCOS visit=1; Date of study=5/4/2016 SUBJECT_SAMPLE_FACTORS 5908 ms6440-19 Time point:135 | group:PCOS visit=1; Date of study=5/4/2016 SUBJECT_SAMPLE_FACTORS 5908 ms6440-20 Time point:150 | group:PCOS visit=1; Date of study=5/4/2016 SUBJECT_SAMPLE_FACTORS 5908 ms6440-21 Time point:165 | group:PCOS visit=1; Date of study=5/4/2016 SUBJECT_SAMPLE_FACTORS 5908 ms6440-22 Time point:180 | group:PCOS visit=1; Date of study=5/4/2016 SUBJECT_SAMPLE_FACTORS 9931 ms6441-12 Time point:baseline | group:PCOS visit=1; Date of study=3/10/2016 SUBJECT_SAMPLE_FACTORS 9931 ms6441-13 Time point:-60 | group:PCOS visit=1; Date of study=3/11/2016 SUBJECT_SAMPLE_FACTORS 9931 ms6441-14 Time point:-45 | group:PCOS visit=1; Date of study=3/11/2016 SUBJECT_SAMPLE_FACTORS 9931 ms6441-15 Time point:-30 | group:PCOS visit=1; Date of study=3/11/2016 SUBJECT_SAMPLE_FACTORS 9931 ms6441-16 Time point:-15 | group:PCOS visit=1; Date of study=3/11/2016 SUBJECT_SAMPLE_FACTORS 9931 ms6441-17 Time point:60 | group:PCOS visit=1; Date of study=3/11/2016 SUBJECT_SAMPLE_FACTORS 9931 ms6441-18 Time point:120 | group:PCOS visit=1; Date of study=3/11/2016 SUBJECT_SAMPLE_FACTORS 9931 ms6441-19 Time point:135 | group:PCOS visit=1; Date of study=3/11/2016 SUBJECT_SAMPLE_FACTORS 9931 ms6441-20 Time point:150 | group:PCOS visit=1; Date of study=3/11/2016 SUBJECT_SAMPLE_FACTORS 9931 ms6441-21 Time point:165 | group:PCOS visit=1; Date of study=3/11/2016 SUBJECT_SAMPLE_FACTORS 9931 ms6441-22 Time point:180 | group:PCOS visit=1; Date of study=3/11/2016 SUBJECT_SAMPLE_FACTORS 4620 ms6442-12 Time point:baseline | group:PCOS visit=1; Date of study=4/21/2016 SUBJECT_SAMPLE_FACTORS 4620 ms6442-13 Time point:-60 | group:PCOS visit=1; Date of study=4/22/2016 SUBJECT_SAMPLE_FACTORS 4620 ms6442-14 Time point:-45 | group:PCOS visit=1; Date of study=4/22/2016 SUBJECT_SAMPLE_FACTORS 4620 ms6442-15 Time point:-30 | group:PCOS visit=1; Date of study=4/22/2016 SUBJECT_SAMPLE_FACTORS 4620 ms6442-16 Time point:-15 | group:PCOS visit=1; Date of study=4/22/2016 SUBJECT_SAMPLE_FACTORS 4620 ms6442-17 Time point:60 | group:PCOS visit=1; Date of study=4/22/2016 SUBJECT_SAMPLE_FACTORS 4620 ms6442-18 Time point:120 | group:PCOS visit=1; Date of study=4/22/2016 SUBJECT_SAMPLE_FACTORS 4620 ms6442-19 Time point:135 | group:PCOS visit=1; Date of study=4/22/2016 SUBJECT_SAMPLE_FACTORS 4620 ms6442-20 Time point:150 | group:PCOS visit=1; Date of study=4/22/2016 SUBJECT_SAMPLE_FACTORS 4620 ms6442-21 Time point:165 | group:PCOS visit=1; Date of study=4/22/2016 SUBJECT_SAMPLE_FACTORS 4620 ms6442-22 Time point:180 | group:PCOS visit=1; Date of study=4/22/2016 SUBJECT_SAMPLE_FACTORS 8404 ms6443-12 Time point:baseline | group:PCOS visit=1; Date of study=3/29/2016 SUBJECT_SAMPLE_FACTORS 8404 ms6443-13 Time point:-60 | group:PCOS visit=1; Date of study=3/30/2016 SUBJECT_SAMPLE_FACTORS 8404 ms6443-14 Time point:-45 | group:PCOS visit=1; Date of study=3/30/2016 SUBJECT_SAMPLE_FACTORS 8404 ms6443-15 Time point:-30 | group:PCOS visit=1; Date of study=3/30/2016 SUBJECT_SAMPLE_FACTORS 8404 ms6443-16 Time point:-15 | group:PCOS visit=1; Date of study=3/30/2016 SUBJECT_SAMPLE_FACTORS 8404 ms6443-17 Time point:60 | group:PCOS visit=1; Date of study=3/30/2016 SUBJECT_SAMPLE_FACTORS 8404 ms6443-18 Time point:120 | group:PCOS visit=1; Date of study=3/30/2016 SUBJECT_SAMPLE_FACTORS 8404 ms6443-19 Time point:135 | group:PCOS visit=1; Date of study=3/30/2016 SUBJECT_SAMPLE_FACTORS 8404 ms6443-20 Time point:150 | group:PCOS visit=1; Date of study=3/30/2016 SUBJECT_SAMPLE_FACTORS 8404 ms6443-21 Time point:165 | group:PCOS visit=1; Date of study=3/30/2016 SUBJECT_SAMPLE_FACTORS 8404 ms6443-22 Time point:180 | group:PCOS visit=1; Date of study=3/30/2016 SUBJECT_SAMPLE_FACTORS 5908 ms6440-1 Time point:baseline | group:PCOS visit=2; Date of study=7/18/2016 SUBJECT_SAMPLE_FACTORS 5908 ms6440-2 Time point:-60 | group:PCOS visit=2; Date of study=7/18/2016 SUBJECT_SAMPLE_FACTORS 5908 ms6440-3 Time point:-45 | group:PCOS visit=2; Date of study=7/18/2016 SUBJECT_SAMPLE_FACTORS 5908 ms6440-4 Time point:-30 | group:PCOS visit=2; Date of study=7/18/2016 SUBJECT_SAMPLE_FACTORS 5908 ms6440-5 Time point:-15 | group:PCOS visit=2; Date of study=7/18/2016 SUBJECT_SAMPLE_FACTORS 5908 ms6440-6 Time point:60 | group:PCOS visit=2; Date of study=7/18/2016 SUBJECT_SAMPLE_FACTORS 5908 ms6440-7 Time point:120 | group:PCOS visit=2; Date of study=7/18/2016 SUBJECT_SAMPLE_FACTORS 5908 ms6440-8 Time point:135 | group:PCOS visit=2; Date of study=7/18/2016 SUBJECT_SAMPLE_FACTORS 5908 ms6440-9 Time point:150 | group:PCOS visit=2; Date of study=7/18/2016 SUBJECT_SAMPLE_FACTORS 5908 ms6440-10 Time point:165 | group:PCOS visit=2; Date of study=7/18/2016 SUBJECT_SAMPLE_FACTORS 5908 ms6440-11 Time point:180 | group:PCOS visit=2; Date of study=7/18/2016 SUBJECT_SAMPLE_FACTORS 9931 ms6441-1 Time point:baseline | group:PCOS visit=2; Date of study=7/12/2016 SUBJECT_SAMPLE_FACTORS 9931 ms6441-2 Time point:-60 | group:PCOS visit=2; Date of study=7/12/2016 SUBJECT_SAMPLE_FACTORS 9931 ms6441-3 Time point:-45 | group:PCOS visit=2; Date of study=7/12/2016 SUBJECT_SAMPLE_FACTORS 9931 ms6441-4 Time point:-30 | group:PCOS visit=2; Date of study=7/12/2016 SUBJECT_SAMPLE_FACTORS 9931 ms6441-5 Time point:-15 | group:PCOS visit=2; Date of study=7/12/2016 SUBJECT_SAMPLE_FACTORS 9931 ms6441-6 Time point:60 | group:PCOS visit=2; Date of study=7/12/2016 SUBJECT_SAMPLE_FACTORS 9931 ms6441-7 Time point:120 | group:PCOS visit=2; Date of study=7/12/2016 SUBJECT_SAMPLE_FACTORS 9931 ms6441-8 Time point:135 | group:PCOS visit=2; Date of study=7/12/2016 SUBJECT_SAMPLE_FACTORS 9931 ms6441-9 Time point:150 | group:PCOS visit=2; Date of study=7/12/2016 SUBJECT_SAMPLE_FACTORS 9931 ms6441-10 Time point:165 | group:PCOS visit=2; Date of study=7/12/2016 SUBJECT_SAMPLE_FACTORS 9931 ms6441-11 Time point:180 | group:PCOS visit=2; Date of study=7/12/2016 SUBJECT_SAMPLE_FACTORS 4620 ms6442-1 Time point:baseline | group:PCOS visit=2; Date of study=8/12/2016 SUBJECT_SAMPLE_FACTORS 4620 ms6442-2 Time point:-60 | group:PCOS visit=2; Date of study=8/12/2016 SUBJECT_SAMPLE_FACTORS 4620 ms6442-3 Time point:-45 | group:PCOS visit=2; Date of study=8/12/2016 SUBJECT_SAMPLE_FACTORS 4620 ms6442-4 Time point:-30 | group:PCOS visit=2; Date of study=8/12/2016 SUBJECT_SAMPLE_FACTORS 4620 ms6442-5 Time point:-15 | group:PCOS visit=2; Date of study=8/12/2016 SUBJECT_SAMPLE_FACTORS 4620 ms6442-6 Time point:60 | group:PCOS visit=2; Date of study=8/12/2016 SUBJECT_SAMPLE_FACTORS 4620 ms6442-7 Time point:120 | group:PCOS visit=2; Date of study=8/12/2016 SUBJECT_SAMPLE_FACTORS 4620 ms6442-8 Time point:135 | group:PCOS visit=2; Date of study=8/12/2016 SUBJECT_SAMPLE_FACTORS 4620 ms6442-9 Time point:150 | group:PCOS visit=2; Date of study=8/12/2016 SUBJECT_SAMPLE_FACTORS 4620 ms6442-10 Time point:165 | group:PCOS visit=2; Date of study=8/12/2016 SUBJECT_SAMPLE_FACTORS 4620 ms6442-11 Time point:180 | group:PCOS visit=2; Date of study=8/12/2016 SUBJECT_SAMPLE_FACTORS 8404 ms6443-1 Time point:baseline | group:PCOS visit=2; Date of study=7/11/2016 SUBJECT_SAMPLE_FACTORS 8404 ms6443-2 Time point:-60 | group:PCOS visit=2; Date of study=7/11/2016 SUBJECT_SAMPLE_FACTORS 8404 ms6443-3 Time point:-45 | group:PCOS visit=2; Date of study=7/11/2016 SUBJECT_SAMPLE_FACTORS 8404 ms6443-4 Time point:-30 | group:PCOS visit=2; Date of study=7/11/2016 SUBJECT_SAMPLE_FACTORS 8404 ms6443-5 Time point:-15 | group:PCOS visit=2; Date of study=7/11/2016 SUBJECT_SAMPLE_FACTORS 8404 ms6443-6 Time point:60 | group:PCOS visit=2; Date of study=7/11/2016 SUBJECT_SAMPLE_FACTORS 8404 ms6443-7 Time point:120 | group:PCOS visit=2; Date of study=7/11/2016 SUBJECT_SAMPLE_FACTORS 8404 ms6443-8 Time point:135 | group:PCOS visit=2; Date of study=7/11/2016 SUBJECT_SAMPLE_FACTORS 8404 ms6443-9 Time point:150 | group:PCOS visit=2; Date of study=7/11/2016 SUBJECT_SAMPLE_FACTORS 8404 ms6443-10 Time point:165 | group:PCOS visit=2; Date of study=7/11/2016 SUBJECT_SAMPLE_FACTORS 8404 ms6443-11 Time point:180 | group:PCOS visit=2; Date of study=7/11/2016 SUBJECT_SAMPLE_FACTORS 3463 ms6712-1 Time point:baseline | group:PCOS visit=1; Date of study=12/2/2015 SUBJECT_SAMPLE_FACTORS 3463 ms6712-2 Time point:-60 | group:PCOS visit=1; Date of study=12/2/2015 SUBJECT_SAMPLE_FACTORS 3463 ms6712-3 Time point:-45 | group:PCOS visit=1; Date of study=12/2/2015 SUBJECT_SAMPLE_FACTORS 3463 ms6712-4 Time point:-30 | group:PCOS visit=1; Date of study=12/2/2015 SUBJECT_SAMPLE_FACTORS 3463 ms6712-5 Time point:-15 | group:PCOS visit=1; Date of study=12/2/2015 SUBJECT_SAMPLE_FACTORS 3463 ms6712-6 Time point:60 | group:PCOS visit=1; Date of study=12/2/2015 SUBJECT_SAMPLE_FACTORS 3463 ms6712-7 Time point:120 | group:PCOS visit=1; Date of study=12/2/2015 SUBJECT_SAMPLE_FACTORS 3463 ms6712-8 Time point:135 | group:PCOS visit=1; Date of study=12/2/2015 SUBJECT_SAMPLE_FACTORS 3463 ms6712-9 Time point:150 | group:PCOS visit=1; Date of study=12/2/2015 SUBJECT_SAMPLE_FACTORS 3463 ms6712-10 Time point:165 | group:PCOS visit=1; Date of study=12/2/2015 SUBJECT_SAMPLE_FACTORS 3463 ms6712-11 Time point:180 | group:PCOS visit=1; Date of study=12/2/2015 SUBJECT_SAMPLE_FACTORS 3463 ms6712-12 Time point:baseline | group:PCOS visit=2; Date of study=3/16/2016 SUBJECT_SAMPLE_FACTORS 3463 ms6712-13 Time point:-60 | group:PCOS visit=2; Date of study=3/16/2016 SUBJECT_SAMPLE_FACTORS 3463 ms6712-14 Time point:-45 | group:PCOS visit=2; Date of study=3/16/2016 SUBJECT_SAMPLE_FACTORS 3463 ms6712-15 Time point:-30 | group:PCOS visit=2; Date of study=3/16/2016 SUBJECT_SAMPLE_FACTORS 3463 ms6712-16 Time point:-15 | group:PCOS visit=2; Date of study=3/16/2016 SUBJECT_SAMPLE_FACTORS 3463 ms6712-17 Time point:60 | group:PCOS visit=2; Date of study=3/16/2016 SUBJECT_SAMPLE_FACTORS 3463 ms6712-18 Time point:120 | group:PCOS visit=2; Date of study=3/16/2016 SUBJECT_SAMPLE_FACTORS 3463 ms6712-19 Time point:135 | group:PCOS visit=2; Date of study=3/16/2016 SUBJECT_SAMPLE_FACTORS 3463 ms6712-20 Time point:150 | group:PCOS visit=2; Date of study=3/16/2016 SUBJECT_SAMPLE_FACTORS 3463 ms6712-21 Time point:165 | group:PCOS visit=2; Date of study=3/16/2016 SUBJECT_SAMPLE_FACTORS 3463 ms6712-22 Time point:180 | group:PCOS visit=2; Date of study=3/16/2016 SUBJECT_SAMPLE_FACTORS 7182 ms6713-1 Time point:baseline | group:PCOS visit=1; Date of study=12/19/2015 SUBJECT_SAMPLE_FACTORS 7182 ms6713-2 Time point:-60 | group:PCOS visit=1; Date of study=12/19/2015 SUBJECT_SAMPLE_FACTORS 7182 ms6713-3 Time point:-45 | group:PCOS visit=1; Date of study=12/19/2015 SUBJECT_SAMPLE_FACTORS 7182 ms6713-4 Time point:-30 | group:PCOS visit=1; Date of study=12/19/2015 SUBJECT_SAMPLE_FACTORS 7182 ms6713-5 Time point:-15 | group:PCOS visit=1; Date of study=12/19/2015 SUBJECT_SAMPLE_FACTORS 7182 ms6713-6 Time point:60 | group:PCOS visit=1; Date of study=12/19/2015 SUBJECT_SAMPLE_FACTORS 7182 ms6713-7 Time point:120 | group:PCOS visit=1; Date of study=12/19/2015 SUBJECT_SAMPLE_FACTORS 7182 ms6713-8 Time point:135 | group:PCOS visit=1; Date of study=12/19/2015 SUBJECT_SAMPLE_FACTORS 7182 ms6713-9 Time point:150 | group:PCOS visit=1; Date of study=12/19/2015 SUBJECT_SAMPLE_FACTORS 7182 ms6713-10 Time point:165 | group:PCOS visit=1; Date of study=12/19/2015 SUBJECT_SAMPLE_FACTORS 7182 ms6713-11 Time point:180 | group:PCOS visit=1; Date of study=12/19/2015 SUBJECT_SAMPLE_FACTORS 7182 ms6713-12 Time point:baseline | group:PCOS visit=2; Date of study=4/9/2016 SUBJECT_SAMPLE_FACTORS 7182 ms6713-13 Time point:-60 | group:PCOS visit=2; Date of study=4/9/2016 SUBJECT_SAMPLE_FACTORS 7182 ms6713-14 Time point:-45 | group:PCOS visit=2; Date of study=4/9/2016 SUBJECT_SAMPLE_FACTORS 7182 ms6713-15 Time point:-30 | group:PCOS visit=2; Date of study=4/9/2016 SUBJECT_SAMPLE_FACTORS 7182 ms6713-16 Time point:-15 | group:PCOS visit=2; Date of study=4/9/2016 SUBJECT_SAMPLE_FACTORS 7182 ms6713-17 Time point:60 | group:PCOS visit=2; Date of study=4/9/2016 SUBJECT_SAMPLE_FACTORS 7182 ms6713-18 Time point:120 | group:PCOS visit=2; Date of study=4/9/2016 SUBJECT_SAMPLE_FACTORS 7182 ms6713-19 Time point:135 | group:PCOS visit=2; Date of study=4/9/2016 SUBJECT_SAMPLE_FACTORS 7182 ms6713-20 Time point:150 | group:PCOS visit=2; Date of study=4/9/2016 SUBJECT_SAMPLE_FACTORS 7182 ms6713-21 Time point:165 | group:PCOS visit=2; Date of study=4/9/2016 SUBJECT_SAMPLE_FACTORS 7182 ms6713-22 Time point:180 | group:PCOS visit=2; Date of study=4/9/2016 SUBJECT_SAMPLE_FACTORS 8999 ms6714-1 Time point:baseline | group:PCOS visit=1; Date of study=12/16/2015 SUBJECT_SAMPLE_FACTORS 8999 ms6714-2 Time point:-60 | group:PCOS visit=1; Date of study=12/16/2015 SUBJECT_SAMPLE_FACTORS 8999 ms6714-3 Time point:-45 | group:PCOS visit=1; Date of study=12/16/2015 SUBJECT_SAMPLE_FACTORS 8999 ms6714-4 Time point:-30 | group:PCOS visit=1; Date of study=12/16/2015 SUBJECT_SAMPLE_FACTORS 8999 ms6714-5 Time point:-15 | group:PCOS visit=1; Date of study=12/16/2015 SUBJECT_SAMPLE_FACTORS 8999 ms6714-6 Time point:60 | group:PCOS visit=1; Date of study=12/16/2015 SUBJECT_SAMPLE_FACTORS 8999 ms6714-7 Time point:120 | group:PCOS visit=1; Date of study=12/16/2015 SUBJECT_SAMPLE_FACTORS 8999 ms6714-8 Time point:135 | group:PCOS visit=1; Date of study=12/16/2015 SUBJECT_SAMPLE_FACTORS 8999 ms6714-9 Time point:150 | group:PCOS visit=1; Date of study=12/16/2015 SUBJECT_SAMPLE_FACTORS 8999 ms6714-10 Time point:165 | group:PCOS visit=1; Date of study=12/16/2015 SUBJECT_SAMPLE_FACTORS 8999 ms6714-11 Time point:180 | group:PCOS visit=1; Date of study=12/16/2015 SUBJECT_SAMPLE_FACTORS 8999 ms6714-12 Time point:baseline | group:PCOS visit=2; Date of study=3/25/2016 SUBJECT_SAMPLE_FACTORS 8999 ms6714-13 Time point:-60 | group:PCOS visit=2; Date of study=3/25/2016 SUBJECT_SAMPLE_FACTORS 8999 ms6714-14 Time point:-45 | group:PCOS visit=2; Date of study=3/25/2016 SUBJECT_SAMPLE_FACTORS 8999 ms6714-15 Time point:-30 | group:PCOS visit=2; Date of study=3/25/2016 SUBJECT_SAMPLE_FACTORS 8999 ms6714-16 Time point:-15 | group:PCOS visit=2; Date of study=3/25/2016 SUBJECT_SAMPLE_FACTORS 8999 ms6714-17 Time point:60 | group:PCOS visit=2; Date of study=3/25/2016 SUBJECT_SAMPLE_FACTORS 8999 ms6714-18 Time point:120 | group:PCOS visit=2; Date of study=3/25/2016 SUBJECT_SAMPLE_FACTORS 8999 ms6714-19 Time point:135 | group:PCOS visit=2; Date of study=3/25/2016 SUBJECT_SAMPLE_FACTORS 8999 ms6714-20 Time point:150 | group:PCOS visit=2; Date of study=3/25/2016 SUBJECT_SAMPLE_FACTORS 8999 ms6714-21 Time point:165 | group:PCOS visit=2; Date of study=3/25/2016 SUBJECT_SAMPLE_FACTORS 8999 ms6714-22 Time point:180 | group:PCOS visit=2; Date of study=3/25/2016 SUBJECT_SAMPLE_FACTORS 7725 ms6715-1 Time point:baseline | group:PCOS visit=1; Date of study=7/14/2016 SUBJECT_SAMPLE_FACTORS 7725 ms6715-2 Time point:-60 | group:PCOS visit=1; Date of study=7/14/2016 SUBJECT_SAMPLE_FACTORS 7725 ms6715-3 Time point:-45 | group:PCOS visit=1; Date of study=7/14/2016 SUBJECT_SAMPLE_FACTORS 7725 ms6715-4 Time point:-30 | group:PCOS visit=1; Date of study=7/14/2016 SUBJECT_SAMPLE_FACTORS 7725 ms6715-5 Time point:-15 | group:PCOS visit=1; Date of study=7/14/2016 SUBJECT_SAMPLE_FACTORS 7725 ms6715-6 Time point:60 | group:PCOS visit=1; Date of study=7/14/2016 SUBJECT_SAMPLE_FACTORS 7725 ms6715-7 Time point:120 | group:PCOS visit=1; Date of study=7/14/2016 SUBJECT_SAMPLE_FACTORS 7725 ms6715-8 Time point:135 | group:PCOS visit=1; Date of study=7/14/2016 SUBJECT_SAMPLE_FACTORS 7725 ms6715-9 Time point:150 | group:PCOS visit=1; Date of study=7/14/2016 SUBJECT_SAMPLE_FACTORS 7725 ms6715-10 Time point:165 | group:PCOS visit=1; Date of study=7/14/2016 SUBJECT_SAMPLE_FACTORS 7725 ms6715-11 Time point:180 | group:PCOS visit=1; Date of study=7/14/2016 SUBJECT_SAMPLE_FACTORS 7725 ms6715-12 Time point:baseline | group:PCOS visit=2; Date of study=10/21/2016 SUBJECT_SAMPLE_FACTORS 7725 ms6715-13 Time point:-60 | group:PCOS visit=2; Date of study=10/21/2016 SUBJECT_SAMPLE_FACTORS 7725 ms6715-14 Time point:-45 | group:PCOS visit=2; Date of study=10/21/2016 SUBJECT_SAMPLE_FACTORS 7725 ms6715-15 Time point:-30 | group:PCOS visit=2; Date of study=10/21/2016 SUBJECT_SAMPLE_FACTORS 7725 ms6715-16 Time point:-15 | group:PCOS visit=2; Date of study=10/21/2016 SUBJECT_SAMPLE_FACTORS 7725 ms6715-17 Time point:60 | group:PCOS visit=2; Date of study=10/21/2016 SUBJECT_SAMPLE_FACTORS 7725 ms6715-18 Time point:120 | group:PCOS visit=2; Date of study=10/21/2016 SUBJECT_SAMPLE_FACTORS 7725 ms6715-19 Time point:135 | group:PCOS visit=2; Date of study=10/21/2016 SUBJECT_SAMPLE_FACTORS 7725 ms6715-20 Time point:150 | group:PCOS visit=2; Date of study=10/21/2016 SUBJECT_SAMPLE_FACTORS 7725 ms6715-21 Time point:165 | group:PCOS visit=2; Date of study=10/21/2016 SUBJECT_SAMPLE_FACTORS 7725 ms6715-22 Time point:180 | group:PCOS visit=2; Date of study=10/21/2016 SUBJECT_SAMPLE_FACTORS 3635 ms6716-1 Time point:baseline | group:PCOS visit=1; Date of study=1/13/2016 SUBJECT_SAMPLE_FACTORS 3635 ms6716-2 Time point:-60 | group:PCOS visit=1; Date of study=1/13/2016 SUBJECT_SAMPLE_FACTORS 3635 ms6716-3 Time point:-45 | group:PCOS visit=1; Date of study=1/13/2016 SUBJECT_SAMPLE_FACTORS 3635 ms6716-4 Time point:-30 | group:PCOS visit=1; Date of study=1/13/2016 SUBJECT_SAMPLE_FACTORS 3635 ms6716-5 Time point:-15 | group:PCOS visit=1; Date of study=1/13/2016 SUBJECT_SAMPLE_FACTORS 3635 ms6716-6 Time point:60 | group:PCOS visit=1; Date of study=1/13/2016 SUBJECT_SAMPLE_FACTORS 3635 ms6716-7 Time point:120 | group:PCOS visit=1; Date of study=1/13/2016 SUBJECT_SAMPLE_FACTORS 3635 ms6716-8 Time point:135 | group:PCOS visit=1; Date of study=1/13/2016 SUBJECT_SAMPLE_FACTORS 3635 ms6716-9 Time point:150 | group:PCOS visit=1; Date of study=1/13/2016 SUBJECT_SAMPLE_FACTORS 3635 ms6716-10 Time point:165 | group:PCOS visit=1; Date of study=1/13/2016 SUBJECT_SAMPLE_FACTORS 3635 ms6716-11 Time point:180 | group:PCOS visit=1; Date of study=1/13/2016 SUBJECT_SAMPLE_FACTORS 3635 ms6716-12 Time point:baseline | group:PCOS visit=2; Date of study=4/30/2016 SUBJECT_SAMPLE_FACTORS 3635 ms6716-13 Time point:-60 | group:PCOS visit=2; Date of study=4/30/2016 SUBJECT_SAMPLE_FACTORS 3635 ms6716-14 Time point:-45 | group:PCOS visit=2; Date of study=4/30/2016 SUBJECT_SAMPLE_FACTORS 3635 ms6716-15 Time point:-30 | group:PCOS visit=2; Date of study=4/30/2016 SUBJECT_SAMPLE_FACTORS 3635 ms6716-16 Time point:-15 | group:PCOS visit=2; Date of study=4/30/2016 SUBJECT_SAMPLE_FACTORS 3635 ms6716-17 Time point:60 | group:PCOS visit=2; Date of study=4/30/2016 SUBJECT_SAMPLE_FACTORS 3635 ms6716-18 Time point:120 | group:PCOS visit=2; Date of study=4/30/2016 SUBJECT_SAMPLE_FACTORS 3635 ms6716-19 Time point:135 | group:PCOS visit=2; Date of study=4/30/2016 SUBJECT_SAMPLE_FACTORS 3635 ms6716-20 Time point:150 | group:PCOS visit=2; Date of study=4/30/2016 SUBJECT_SAMPLE_FACTORS 3635 ms6716-21 Time point:165 | group:PCOS visit=2; Date of study=4/30/2016 SUBJECT_SAMPLE_FACTORS 3635 ms6716-22 Time point:180 | group:PCOS visit=2; Date of study=4/30/2016 SUBJECT_SAMPLE_FACTORS 9103 ms6717-1 Time point:baseline | group:PCOS visit=1; Date of study=8/1/2016 SUBJECT_SAMPLE_FACTORS 9103 ms6717-2 Time point:-60 | group:PCOS visit=1; Date of study=8/1/2016 SUBJECT_SAMPLE_FACTORS 9103 ms6717-3 Time point:-45 | group:PCOS visit=1; Date of study=8/1/2016 SUBJECT_SAMPLE_FACTORS 9103 ms6717-4 Time point:-30 | group:PCOS visit=1; Date of study=8/1/2016 SUBJECT_SAMPLE_FACTORS 9103 ms6717-5 Time point:-15 | group:PCOS visit=1; Date of study=8/1/2016 SUBJECT_SAMPLE_FACTORS 9103 ms6717-6 Time point:60 | group:PCOS visit=1; Date of study=8/1/2016 SUBJECT_SAMPLE_FACTORS 9103 ms6717-7 Time point:120 | group:PCOS visit=1; Date of study=8/1/2016 SUBJECT_SAMPLE_FACTORS 9103 ms6717-8 Time point:135 | group:PCOS visit=1; Date of study=8/1/2016 SUBJECT_SAMPLE_FACTORS 9103 ms6717-9 Time point:150 | group:PCOS visit=1; Date of study=8/1/2016 SUBJECT_SAMPLE_FACTORS 9103 ms6717-10 Time point:165 | group:PCOS visit=1; Date of study=8/1/2016 SUBJECT_SAMPLE_FACTORS 9103 ms6717-11 Time point:180 | group:PCOS visit=1; Date of study=8/1/2016 SUBJECT_SAMPLE_FACTORS 9103 ms6717-12 Time point:baseline | group:PCOS visit=2; Date of study=11/14/2016 SUBJECT_SAMPLE_FACTORS 9103 ms6717-13 Time point:-60 | group:PCOS visit=2; Date of study=11/14/2016 SUBJECT_SAMPLE_FACTORS 9103 ms6717-14 Time point:-45 | group:PCOS visit=2; Date of study=11/14/2016 SUBJECT_SAMPLE_FACTORS 9103 ms6717-15 Time point:-30 | group:PCOS visit=2; Date of study=11/14/2016 SUBJECT_SAMPLE_FACTORS 9103 ms6717-16 Time point:-15 | group:PCOS visit=2; Date of study=11/14/2016 SUBJECT_SAMPLE_FACTORS 9103 ms6717-17 Time point:60 | group:PCOS visit=2; Date of study=11/14/2016 SUBJECT_SAMPLE_FACTORS 9103 ms6717-18 Time point:120 | group:PCOS visit=2; Date of study=11/14/2016 SUBJECT_SAMPLE_FACTORS 9103 ms6717-19 Time point:135 | group:PCOS visit=2; Date of study=11/14/2016 SUBJECT_SAMPLE_FACTORS 9103 ms6717-20 Time point:150 | group:PCOS visit=2; Date of study=11/14/2016 SUBJECT_SAMPLE_FACTORS 9103 ms6717-21 Time point:165 | group:PCOS visit=2; Date of study=11/14/2016 SUBJECT_SAMPLE_FACTORS 9103 ms6717-22 Time point:180 | group:PCOS visit=2; Date of study=11/14/2016 SUBJECT_SAMPLE_FACTORS 6936 ms6718-1 Time point:baseline | group:PCOS visit=1; Date of study=7/28/2016 SUBJECT_SAMPLE_FACTORS 6936 ms6718-2 Time point:-60 | group:PCOS visit=1; Date of study=7/28/2016 SUBJECT_SAMPLE_FACTORS 6936 ms6718-3 Time point:-45 | group:PCOS visit=1; Date of study=7/28/2016 SUBJECT_SAMPLE_FACTORS 6936 ms6718-4 Time point:-30 | group:PCOS visit=1; Date of study=7/28/2016 SUBJECT_SAMPLE_FACTORS 6936 ms6718-5 Time point:-15 | group:PCOS visit=1; Date of study=7/28/2016 SUBJECT_SAMPLE_FACTORS 6936 ms6718-6 Time point:60 | group:PCOS visit=1; Date of study=7/28/2016 SUBJECT_SAMPLE_FACTORS 6936 ms6718-7 Time point:120 | group:PCOS visit=1; Date of study=7/28/2016 SUBJECT_SAMPLE_FACTORS 6936 ms6718-8 Time point:135 | group:PCOS visit=1; Date of study=7/28/2016 SUBJECT_SAMPLE_FACTORS 6936 ms6718-9 Time point:150 | group:PCOS visit=1; Date of study=7/28/2016 SUBJECT_SAMPLE_FACTORS 6936 ms6718-10 Time point:165 | group:PCOS visit=1; Date of study=7/28/2016 SUBJECT_SAMPLE_FACTORS 6936 ms6718-11 Time point:180 | group:PCOS visit=1; Date of study=42579 SUBJECT_SAMPLE_FACTORS 6936 ms6718-12 Time point:baseline | group:PCOS visit=2; Date of study=42677 SUBJECT_SAMPLE_FACTORS 6936 ms6718-13 Time point:-60 | group:PCOS visit=2; Date of study=42677 SUBJECT_SAMPLE_FACTORS 6936 ms6718-14 Time point:-45 | group:PCOS visit=2; Date of study=42677 SUBJECT_SAMPLE_FACTORS 6936 ms6718-15 Time point:-30 | group:PCOS visit=2; Date of study=42677 SUBJECT_SAMPLE_FACTORS 6936 ms6718-16 Time point:-15 | group:PCOS visit=2; Date of study=42677 SUBJECT_SAMPLE_FACTORS 6936 ms6718-17 Time point:60 | group:PCOS visit=2; Date of study=42677 SUBJECT_SAMPLE_FACTORS 6936 ms6718-18 Time point:120 | group:PCOS visit=2; Date of study=42677 SUBJECT_SAMPLE_FACTORS 6936 ms6718-19 Time point:135 | group:PCOS visit=2; Date of study=42677 SUBJECT_SAMPLE_FACTORS 6936 ms6718-20 Time point:150 | group:PCOS visit=2; Date of study=42677 SUBJECT_SAMPLE_FACTORS 6936 ms6718-21 Time point:165 | group:PCOS visit=2; Date of study=42677 SUBJECT_SAMPLE_FACTORS 6936 ms6718-22 Time point:180 | group:PCOS visit=2; Date of study=42677 #COLLECTION CO:COLLECTION_SUMMARY "We will perform a hyperinsulinemic-euglycemic clamp as previously described CO:COLLECTION_SUMMARY except that in this current proposal we will perform the study for 3 hours.(36) CO:COLLECTION_SUMMARY The goal for this infusion is to assess the effect of hyperinsulinemia on lysine CO:COLLECTION_SUMMARY and AAA kinetics and whether metformin changes the effect of hyperinsulinemia. CO:COLLECTION_SUMMARY We will collect samples every 10 min for glucose. 40% dextrose will be infused CO:COLLECTION_SUMMARY at a variable rate during the clamp to maintain euglycemia.(36) During the last CO:COLLECTION_SUMMARY hour of the clamp, 5 blood samples will be drawn for measurement of stable CO:COLLECTION_SUMMARY isotope tracer enrichment and amino acid concentrations. Previous studies have CO:COLLECTION_SUMMARY established that the combination of metformin and pioglitazone decrease lysine CO:COLLECTION_SUMMARY and AAA concentrations in overweight and obese adults with impaired fasting CO:COLLECTION_SUMMARY glucose or untreated diabetes.(7) Aim 3 is designed to establish whether changes CO:COLLECTION_SUMMARY in lysine and AAA flux are associated with changes in insulin sensitivity. CO:COLLECTION_SUMMARY Measurement of lysine and AAA flux will be repeated in 10 women with PCOS after CO:COLLECTION_SUMMARY three months of metformin and 10 women with PCOS randomized to receive no CO:COLLECTION_SUMMARY treatment for three months. Insulin sensitivity and other measures of glucose CO:COLLECTION_SUMMARY metabolism will be calculated from an oral glucose tolerance test using the oral CO:COLLECTION_SUMMARY minimal model as described below." CO:SAMPLE_TYPE Blood (plasma) #TREATMENT TR:TREATMENT_SUMMARY "Ten women with PCOS will receive metformin therapy, and ten women will be TR:TREATMENT_SUMMARY randomized to receive no therapy and undergo the same repeat visits after 3 TR:TREATMENT_SUMMARY months. Ten age-matched women without PCOS with a BMI < 25 will be recruited as TR:TREATMENT_SUMMARY the control group for the baseline visit. Aside from criteria that they do not TR:TREATMENT_SUMMARY have PCOS and have a BMI < 25, this control group will have the same inclusion TR:TREATMENT_SUMMARY and exclusion criteria below. They will not receive metformin and will not TR:TREATMENT_SUMMARY return for repeat visits after 3 months. Metformin therapy: Previous studies TR:TREATMENT_SUMMARY with metformin demonstrated improvement in insulin sensitivity as early as 3 TR:TREATMENT_SUMMARY months with 1000 mg daily.(5, 33, 34) Metformin will be initiated with 500 mg TR:TREATMENT_SUMMARY extended-release tablet daily for one week, 1000 mg daily for one week and then TR:TREATMENT_SUMMARY 1500 mg daily. Visits 4 and 5 will be conducted three months after full dose is TR:TREATMENT_SUMMARY achieved. Oral glucose tolerance test: After 2 baseline fasting samples, 75 g of TR:TREATMENT_SUMMARY oral dextrose will be ingested with blood samples will be drawn at 10’, 20’, TR:TREATMENT_SUMMARY 30’, 60’, 90’, 120’, 150’and 180’ for measurement of glucose, TR:TREATMENT_SUMMARY insulin, c-peptide. Insulin sensitivity will be calculated using the oral TR:TREATMENT_SUMMARY glucose minimal model. Stable isotope tracer infusions (Figure 5): Three days TR:TREATMENT_SUMMARY prior to the tracer study, the participants will be placed on a TR:TREATMENT_SUMMARY weight-maintaining diet consisting of 50% carbohydrates, 20% protein, and 30% TR:TREATMENT_SUMMARY fats. Fat free mass (FFM) measured by dual-energy x-ray absorptiometry will be TR:TREATMENT_SUMMARY used for dose calculations of the stable isotope tracers and insulin infusions TR:TREATMENT_SUMMARY for the hyperinsulinemic-euglycemic clamp. A priming bolus dose of TR:TREATMENT_SUMMARY L-[α-15N]-lysine, 3 to 5 μmol/kg FFM, will be given at the start of a 3 hour TR:TREATMENT_SUMMARY infusion of 3 to 5 μmol/kg FFM/hr to achieve a plateau as previously TR:TREATMENT_SUMMARY described.(29) At the same time, a priming bolus of 1 to 2 μmol/kg FFM TR:TREATMENT_SUMMARY L-[1-13C]-2-aminoadipic acid will be given followed by infusion of 1 to 2 TR:TREATMENT_SUMMARY μmol/kg/hr based on prior study.(30) A retrograde hand intravenous line will be TR:TREATMENT_SUMMARY placed with the hand placed in a warm box maintained at 140°F to obtain TR:TREATMENT_SUMMARY arterialized venous blood samples for measurement of lysine and AAA TR:TREATMENT_SUMMARY concentrations and stable isotopic enrichment at steady state and during the TR:TREATMENT_SUMMARY clamp. Hyperinsulinemic-euglycemic clamp: We will perform a TR:TREATMENT_SUMMARY hyperinsulinemic-euglycemic clamp as previously described except that in this TR:TREATMENT_SUMMARY current proposal we will perform the study for 3 hours.(36) The goal for this TR:TREATMENT_SUMMARY infusion is to assess the effect of hyperinsulinemia on lysine and AAA kinetics TR:TREATMENT_SUMMARY and whether metformin changes the effect of hyperinsulinemia. We will collect TR:TREATMENT_SUMMARY samples every 10 min for glucose. 40% dextrose will be infused at a variable TR:TREATMENT_SUMMARY rate during the clamp to maintain euglycemia.(36) During the last hour of the TR:TREATMENT_SUMMARY clamp, 5 blood samples will be drawn for measurement of stable isotope tracer TR:TREATMENT_SUMMARY enrichment and amino acid concentrations. " #SAMPLEPREP SP:SAMPLEPREP_SUMMARY Plasma lysine and AAA concentrations and isotopic enrichment will be analyzed as SP:SAMPLEPREP_SUMMARY previous described via a Thermo Fisher Q Exactive plus mass spectrometer coupled SP:SAMPLEPREP_SUMMARY with a Dionex UltiMate 3000 Binary RSLC liquid chromatograph. #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE Reversed phase CH:INSTRUMENT_NAME Thermo Dionex Ultimate 3000 CH:COLUMN_NAME Unspecified #ANALYSIS AN:ANALYSIS_TYPE MS #MS MS:INSTRUMENT_NAME Thermo Q Exactive Plus Orbitrap MS:INSTRUMENT_TYPE Orbitrap MS:MS_TYPE ESI MS:ION_MODE POSITIVE MS:MS_COMMENTS uM #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS uM MS_METABOLITE_DATA_START Samples ms6436-12 ms6436-13 ms6436-14 ms6436-15 ms6436-16 ms6436-17 ms6436-18 ms6436-19 ms6436-20 ms6436-21 ms6436-22 ms6437-12 ms6437-13 ms6437-14 ms6437-15 ms6437-16 ms6437-17 ms6437-18 ms6437-19 ms6437-20 ms6437-21 ms6437-22 ms6438-12 ms6438-13 ms6438-14 ms6438-15 ms6438-16 ms6438-17 ms6438-18 ms6438-19 ms6438-20 ms6438-21 ms6438-22 ms6439-12 ms6439-13 ms6439-14 ms6439-15 ms6439-16 ms6439-17 ms6439-18 ms6439-19 ms6439-20 ms6439-21 ms6439-22 ms6436-1 ms6436-2 ms6436-3 ms6436-4 ms6436-5 ms6436-6 ms6436-7 ms6436-8 ms6436-9 ms6436-10 ms6436-11 ms6437-1 ms6437-2 ms6437-3 ms6437-4 ms6437-5 ms6437-6 ms6437-7 ms6437-8 ms6437-9 ms6437-10 ms6437-11 ms6438-1 ms6438-2 ms6438-3 ms6438-4 ms6438-5 ms6438-6 ms6438-7 ms6438-8 ms6438-9 ms6438-10 ms6438-11 ms6439-1 ms6439-2 ms6439-3 ms6439-4 ms6439-5 ms6439-6 ms6439-7 ms6439-8 ms6439-9 ms6439-10 ms6439-11 ms6440-12 ms6440-13 ms6440-14 ms6440-15 ms6440-16 ms6440-17 ms6440-18 ms6440-19 ms6440-20 ms6440-21 ms6440-22 ms6441-12 ms6441-13 ms6441-14 ms6441-15 ms6441-16 ms6441-17 ms6441-18 ms6441-19 ms6441-20 ms6441-21 ms6441-22 ms6442-12 ms6442-13 ms6442-14 ms6442-15 ms6442-16 ms6442-17 ms6442-18 ms6442-19 ms6442-20 ms6442-21 ms6442-22 ms6443-12 ms6443-13 ms6443-14 ms6443-15 ms6443-16 ms6443-17 ms6443-18 ms6443-19 ms6443-20 ms6443-21 ms6443-22 ms6440-1 ms6440-2 ms6440-3 ms6440-4 ms6440-5 ms6440-6 ms6440-7 ms6440-8 ms6440-9 ms6440-10 ms6440-11 ms6441-1 ms6441-2 ms6441-3 ms6441-4 ms6441-5 ms6441-6 ms6441-7 ms6441-8 ms6441-9 ms6441-10 ms6441-11 ms6442-1 ms6442-2 ms6442-3 ms6442-4 ms6442-5 ms6442-6 ms6442-7 ms6442-8 ms6442-9 ms6442-10 ms6442-11 ms6443-1 ms6443-2 ms6443-3 ms6443-4 ms6443-5 ms6443-6 ms6443-7 ms6443-8 ms6443-9 ms6443-10 ms6443-11 ms6712-1 ms6712-2 ms6712-3 ms6712-4 ms6712-5 ms6712-6 ms6712-7 ms6712-8 ms6712-9 ms6712-10 ms6712-11 ms6712-12 ms6712-13 ms6712-14 ms6712-15 ms6712-16 ms6712-17 ms6712-18 ms6712-19 ms6712-20 ms6712-21 ms6712-22 ms6713-1 ms6713-2 ms6713-3 ms6713-4 ms6713-5 ms6713-6 ms6713-7 ms6713-8 ms6713-9 ms6713-10 ms6713-11 ms6713-12 ms6713-13 ms6713-14 ms6713-15 ms6713-16 ms6713-17 ms6713-18 ms6713-19 ms6713-20 ms6713-21 ms6713-22 ms6714-1 ms6714-2 ms6714-3 ms6714-4 ms6714-5 ms6714-6 ms6714-7 ms6714-8 ms6714-9 ms6714-10 ms6714-11 ms6714-12 ms6714-13 ms6714-14 ms6714-15 ms6714-16 ms6714-17 ms6714-18 ms6714-19 ms6714-20 ms6714-21 ms6714-22 ms6715-1 ms6715-2 ms6715-3 ms6715-4 ms6715-5 ms6715-6 ms6715-7 ms6715-8 ms6715-9 ms6715-10 ms6715-11 ms6715-12 ms6715-13 ms6715-14 ms6715-15 ms6715-16 ms6715-17 ms6715-18 ms6715-19 ms6715-20 ms6715-21 ms6715-22 ms6716-1 ms6716-2 ms6716-3 ms6716-4 ms6716-5 ms6716-6 ms6716-7 ms6716-8 ms6716-9 ms6716-10 ms6716-11 ms6716-12 ms6716-13 ms6716-14 ms6716-15 ms6716-16 ms6716-17 ms6716-18 ms6716-19 ms6716-20 ms6716-21 ms6716-22 ms6717-1 ms6717-2 ms6717-3 ms6717-4 ms6717-5 ms6717-6 ms6717-7 ms6717-8 ms6717-9 ms6717-10 ms6717-11 ms6717-12 ms6717-13 ms6717-14 ms6717-15 ms6717-16 ms6717-17 ms6717-18 ms6717-19 ms6717-20 ms6717-21 ms6717-22 ms6718-1 ms6718-2 ms6718-3 ms6718-4 ms6718-5 ms6718-6 ms6718-7 ms6718-8 ms6718-9 ms6718-10 ms6718-11 ms6718-12 ms6718-13 ms6718-14 ms6718-15 ms6718-16 ms6718-17 ms6718-18 ms6718-19 ms6718-20 ms6718-21 ms6718-22 Factors Time point:baseline | group:PCOS Time point:-60 | group:PCOS Time point:-45 | group:PCOS Time point:-30 | group:PCOS Time point:-15 | group:PCOS Time point:60 | group:PCOS Time point:120 | group:PCOS Time point:135 | group:PCOS Time point:150 | group:PCOS Time point:165 | group:PCOS Time point:180 | group:PCOS Time point:baseline | group:PCOS Time point:-60 | group:PCOS Time point:-45 | group:PCOS Time point:-30 | group:PCOS Time point:-15 | group:PCOS Time point:60 | group:PCOS Time point:120 | group:PCOS Time point:135 | group:PCOS Time point:150 | group:PCOS Time point:165 | group:PCOS Time point:180 | group:PCOS Time point:baseline | group:PCOS Time point:-60 | group:PCOS Time point:-45 | group:PCOS Time point:-30 | group:PCOS Time point:-15 | group:PCOS Time point:60 | group:PCOS Time point:120 | group:PCOS Time point:135 | group:PCOS Time point:150 | group:PCOS Time point:165 | group:PCOS Time point:180 | group:PCOS Time point:baseline | group:PCOS Time point:-60 | group:PCOS Time point:-45 | group:PCOS Time point:-30 | group:PCOS Time point:-15 | group:PCOS Time point:60 | group:PCOS Time point:120 | group:PCOS Time point:135 | group:PCOS Time point:150 | group:PCOS Time point:165 | group:PCOS Time point:180 | group:PCOS Time point:baseline | group:PCOS Time point:-60 | group:PCOS Time point:-45 | group:PCOS Time point:-30 | group:PCOS Time point:-15 | group:PCOS Time point:60 | group:PCOS Time point:120 | group:PCOS Time point:135 | group:PCOS Time point:150 | group:PCOS Time point:165 | group:PCOS Time point:180 | group:PCOS Time point:baseline | group:PCOS Time point:-60 | group:PCOS Time point:-45 | group:PCOS Time point:-30 | group:PCOS Time point:-15 | group:PCOS Time point:60 | group:PCOS Time point:120 | group:PCOS Time point:135 | group:PCOS Time point:150 | group:PCOS Time point:165 | group:PCOS Time point:180 | group:PCOS Time point:baseline | group:PCOS Time point:-60 | group:PCOS Time point:-45 | group:PCOS Time point:-30 | group:PCOS Time point:-15 | group:PCOS Time point:60 | group:PCOS Time point:120 | group:PCOS Time point:135 | group:PCOS Time point:150 | group:PCOS Time point:165 | group:PCOS Time point:180 | group:PCOS Time point:baseline | group:PCOS Time point:-60 | group:PCOS Time point:-45 | group:PCOS Time point:-30 | group:PCOS Time point:-15 | group:PCOS Time point:60 | group:PCOS Time point:120 | group:PCOS Time point:135 | group:PCOS Time point:150 | group:PCOS Time point:165 | group:PCOS Time point:180 | group:PCOS Time point:baseline | group:PCOS Time point:-60 | group:PCOS Time point:-45 | group:PCOS Time point:-30 | group:PCOS Time point:-15 | group:PCOS Time point:60 | group:PCOS Time point:120 | group:PCOS Time point:135 | group:PCOS Time point:150 | group:PCOS Time point:165 | group:PCOS Time point:180 | group:PCOS Time point:baseline | group:PCOS Time point:-60 | group:PCOS Time point:-45 | group:PCOS Time point:-30 | group:PCOS Time point:-15 | group:PCOS Time point:60 | group:PCOS Time point:120 | group:PCOS Time point:135 | group:PCOS Time point:150 | group:PCOS Time point:165 | group:PCOS Time point:180 | group:PCOS Time point:baseline | group:PCOS Time point:-60 | group:PCOS Time point:-45 | group:PCOS Time point:-30 | group:PCOS Time point:-15 | group:PCOS Time point:60 | group:PCOS Time point:120 | group:PCOS Time point:135 | group:PCOS Time point:150 | group:PCOS Time point:165 | group:PCOS Time point:180 | group:PCOS Time point:baseline | group:PCOS Time point:-60 | group:PCOS Time point:-45 | group:PCOS Time point:-30 | group:PCOS Time point:-15 | group:PCOS Time point:60 | group:PCOS Time point:120 | group:PCOS Time point:135 | group:PCOS Time point:150 | group:PCOS Time point:165 | group:PCOS Time point:180 | group:PCOS Time point:baseline | group:PCOS Time point:-60 | group:PCOS Time point:-45 | group:PCOS Time point:-30 | group:PCOS Time point:-15 | group:PCOS Time point:60 | group:PCOS Time point:120 | group:PCOS Time point:135 | group:PCOS Time point:150 | group:PCOS Time point:165 | group:PCOS Time point:180 | group:PCOS Time point:baseline | group:PCOS Time point:-60 | group:PCOS Time point:-45 | group:PCOS Time point:-30 | group:PCOS Time point:-15 | group:PCOS Time point:60 | group:PCOS Time point:120 | group:PCOS Time point:135 | group:PCOS Time point:150 | group:PCOS Time point:165 | group:PCOS Time point:180 | group:PCOS Time point:baseline | group:PCOS Time point:-60 | group:PCOS Time point:-45 | group:PCOS Time point:-30 | group:PCOS Time point:-15 | group:PCOS Time point:60 | group:PCOS Time point:120 | group:PCOS Time point:135 | group:PCOS Time point:150 | group:PCOS Time point:165 | group:PCOS Time point:180 | group:PCOS Time point:baseline | group:PCOS Time point:-60 | group:PCOS Time point:-45 | group:PCOS Time point:-30 | group:PCOS Time point:-15 | group:PCOS Time point:60 | group:PCOS Time point:120 | group:PCOS Time point:135 | group:PCOS Time point:150 | group:PCOS Time point:165 | group:PCOS Time point:180 | group:PCOS Time point:baseline | group:PCOS Time point:-60 | group:PCOS Time point:-45 | group:PCOS Time point:-30 | group:PCOS Time point:-15 | group:PCOS Time point:60 | group:PCOS Time point:120 | group:PCOS Time point:135 | group:PCOS Time point:150 | group:PCOS Time point:165 | group:PCOS Time point:180 | group:PCOS Time point:baseline | group:PCOS Time point:-60 | group:PCOS Time point:-45 | group:PCOS Time point:-30 | group:PCOS Time point:-15 | group:PCOS Time point:60 | group:PCOS Time point:120 | group:PCOS Time point:135 | group:PCOS Time point:150 | group:PCOS Time point:165 | group:PCOS Time point:180 | group:PCOS Time point:baseline | group:PCOS Time point:-60 | group:PCOS Time point:-45 | group:PCOS Time point:-30 | group:PCOS Time point:-15 | group:PCOS Time point:60 | group:PCOS Time point:120 | group:PCOS Time point:135 | group:PCOS Time point:150 | group:PCOS Time point:165 | group:PCOS Time point:180 | group:PCOS Time point:baseline | group:PCOS Time point:-60 | group:PCOS Time point:-45 | group:PCOS Time point:-30 | group:PCOS Time point:-15 | group:PCOS Time point:60 | group:PCOS Time point:120 | group:PCOS Time point:135 | group:PCOS Time point:150 | group:PCOS Time point:165 | group:PCOS Time point:180 | group:PCOS Time point:baseline | group:PCOS Time point:-60 | group:PCOS Time point:-45 | group:PCOS Time point:-30 | group:PCOS Time point:-15 | group:PCOS Time point:60 | group:PCOS Time point:120 | group:PCOS Time point:135 | group:PCOS Time point:150 | group:PCOS Time point:165 | group:PCOS Time point:180 | group:PCOS Time point:baseline | group:PCOS Time point:-60 | group:PCOS Time point:-45 | group:PCOS Time point:-30 | group:PCOS Time point:-15 | group:PCOS Time point:60 | group:PCOS Time point:120 | group:PCOS Time point:135 | group:PCOS Time point:150 | group:PCOS Time point:165 | group:PCOS Time point:180 | group:PCOS Time point:baseline | group:PCOS Time point:-60 | group:PCOS Time point:-45 | group:PCOS Time point:-30 | group:PCOS Time point:-15 | group:PCOS Time point:60 | group:PCOS Time point:120 | group:PCOS Time point:135 | group:PCOS Time point:150 | group:PCOS Time point:165 | group:PCOS Time point:180 | group:PCOS Time point:baseline | group:PCOS Time point:-60 | group:PCOS Time point:-45 | group:PCOS Time point:-30 | group:PCOS Time point:-15 | group:PCOS Time point:60 | group:PCOS Time point:120 | group:PCOS Time point:135 | group:PCOS Time point:150 | group:PCOS Time point:165 | group:PCOS Time point:180 | group:PCOS Time point:baseline | group:PCOS Time point:-60 | group:PCOS Time point:-45 | group:PCOS Time point:-30 | group:PCOS Time point:-15 | group:PCOS Time point:60 | group:PCOS Time point:120 | group:PCOS Time point:135 | group:PCOS Time point:150 | group:PCOS Time point:165 | group:PCOS Time point:180 | group:PCOS Time point:baseline | group:PCOS Time point:-60 | group:PCOS Time point:-45 | group:PCOS Time point:-30 | group:PCOS Time point:-15 | group:PCOS Time point:60 | group:PCOS Time point:120 | group:PCOS Time point:135 | group:PCOS Time point:150 | group:PCOS Time point:165 | group:PCOS Time point:180 | group:PCOS Time point:baseline | group:PCOS Time point:-60 | group:PCOS Time point:-45 | group:PCOS Time point:-30 | group:PCOS Time point:-15 | group:PCOS Time point:60 | group:PCOS Time point:120 | group:PCOS Time point:135 | group:PCOS Time point:150 | group:PCOS Time point:165 | group:PCOS Time point:180 | group:PCOS Time point:baseline | group:PCOS Time point:-60 | group:PCOS Time point:-45 | group:PCOS Time point:-30 | group:PCOS Time point:-15 | group:PCOS Time point:60 | group:PCOS Time point:120 | group:PCOS Time point:135 | group:PCOS Time point:150 | group:PCOS Time point:165 | group:PCOS Time point:180 | group:PCOS Time point:baseline | group:PCOS Time point:-60 | group:PCOS Time point:-45 | group:PCOS Time point:-30 | group:PCOS Time point:-15 | group:PCOS Time point:60 | group:PCOS Time point:120 | group:PCOS Time point:135 | group:PCOS Time point:150 | group:PCOS Time point:165 | group:PCOS Time point:180 | group:PCOS Time point:baseline | group:PCOS Time point:-60 | group:PCOS Time point:-45 | group:PCOS Time point:-30 | group:PCOS Time point:-15 | group:PCOS Time point:60 | group:PCOS Time point:120 | group:PCOS Time point:135 | group:PCOS Time point:150 | group:PCOS Time point:165 | group:PCOS Time point:180 | group:PCOS aminoadipic 1.165605023 0.960399004 0.957366386 0.935123831 0.789500425 0.689340973 0.520374439 0.654354673 0.598773698 0.723580862 0.647846515 1.397621633 0.668405583 0.633304836 0.608809724 0.620805912 0.470184945 0.479470363 0.551693149 0.618034409 0.569324058 0.510231458 1.459662029 1.087925608 1.124455 1.306361189 1.230513314 1.005061876 0.814505341 1.042006689 1.025575085 0.977779846 1.04283407 1.456292372 1.124608299 0.962185149 1.202013609 1.368985956 1.259130112 1.024536056 1.138101735 1.036574056 1.186907217 1.033213294 1.047156915 0.787492134 0.761325252 0.90963562 0.775581265 0.690722624 0.642132256 0.664363593 0.645262499 0.611606511 0.633949119 1.444804327 0.51058234 0.534725864 0.551662942 0.593066762 0.526337349 0.477124499 0.723012715 0.54996738 0.555381828 0.639195908 1.900166792 1.161349674 1.162606878 1.126740713 1.48478205 1.304800904 0.974396374 0.910465783 0.85656035 0.985345602 0.909058188 1.417891756 1.051037272 1.239165669 1.203856163 1.130863097 1.337877789 0.997476125 1.162335513 0.992083149 0.977833541 1.109194416 0.845355235 0.982350664 1.320307849 1.130643504 1.041831381 0.937630688 0.696498829 0.858578323 0.773483702 0.901068389 0.551122692 1.751506252 0.837075056 0.782376623 0.862418128 0.759029157 0.794523326 0.804241201 0.687191099 0.589299211 0.734562594 0.700808352 1.509419961 0.88420231 0.834254569 0.860894159 0.887332957 0.690077444 0.558827105 0.58764375 0.588848178 0.579470859 0.672504613 1.259297082 0.988582303 0.760841569 1.000165715 1.165396547 0.669501524 0.610090995 0.613318851 0.833968531 0.844755058 0.958248961 1.770796374 1.094134123 1.206760047 1.140627424 0.94 1.016657566 0.660485256 0.953093132 0.801038245 0.889219771 0.747142525 0.971086583 0.850830506 0.96531954 0.926354395 0.947041282 0.95323872 0.742363249 0.911694303 0.761940989 0.776049492 0.863158009 1.340592285 0.77529796 0.702518833 0.708583546 0.601131146 0.593147748 0.544406483 0.560003507 0.512443691 0.505457833 0.562602741 1.250942998 0.932516019 0.982833377 1.093066151 0.9699594 0.788451549 0.600866499 0.794224812 1.024473461 0.541638565 0.686488646 1.056500863 1.009422564 0.966756069 0.805314322 0.820671201 0.695058565 0.674013447 0.578565026 0.591149477 0.591402437 0.665378102 1.034352974 1.008536619 0.815357003 0.7616398 0.833293511 0.822442651 0.608896186 0.586299896 0.635694529 0.65577687 0.790360214 0.595933929 0.706016766 0.793808877 0.820521916 0.837881713 0.564362819 0.487216325 0.733833567 0.691947748 0.571733588 0.570513688 0.853340229 0.748156429 0.72079718 0.815051682 0.699558825 0.969562268 0.562553758 0.80039102 0.533553859 0.734933335 0.387557915 1.167728858 0.904407609 0.868908969 0.963017354 0.834523285 0.76578535 0.606522176 0.692658262 0.684165438 0.545059639 0.622676074 1.058035723 0.907507068 0.848783894 0.803315723 0.669557858 0.681433613 0.512349461 0.626767621 0.494742621 0.642895173 0.622621844 1.753863291 1.345975298 1.329462093 1.312362809 1.254870733 1.190890379 0.986437845 1.144366793 1.127404941 1.090865175 0.997784736 1.69117582 1.074708784 0.995367997 1.124872803 0.94860888 0.95219874 0.588148913 0.902938747 0.964182299 1.180781778 0.837793473 0.582996857 0.391950594 0.429895992 0.476721233 0.464303407 0.386260075 0.3262776 0.370698606 0.346062271 0.403505853 0.356436687 0.412014039 0.33547514 0.393335781 0.338487153 0.409661368 0.410740771 0.33047343 0.366787165 0.351751185 0.391393937 0.374115897 1.519477815 0.848782018 0.921254486 0.925073928 0.918632688 0.676172188 0.561613325 0.597380612 0.664794352 0.697550401 0.712790637 1.166628859 0.590878289 0.510871609 0.62484124 0.483348329 0.388104327 0.435842135 0.469448265 0.378941281 0.531293763 2.314155767 1.000821586 1.0831644 0.976585387 0.98358207 0.858127446 0.721035965 0.817812479 0.758030693 1.034495585 0.589782324 1.826174873 1.040569551 1.147663995 1.020502801 0.93275654 0.889707039 0.757693918 0.925297353 0.983414191 0.833523808 0.824989251 Lysine 198.6328475 168.5092306 162.6808553 165.2957778 148.252891 164.3198726 147.0176239 154.6236175 157.7335335 160.6053623 157.1740848 261.6634987 181.3724291 201.4773171 175.6544568 186.5811251 167.5651645 169.4490412 181.4992784 185.8383756 174.822679 192.1222181 203.5715281 214.9079188 200.6150984 226.4399697 219.2399372 195.2775979 188.7185062 197.3630329 195.0558856 197.5147314 203.4330811 273.0689428 269.8362317 261.6429019 272.9457953 277.1949968 258.4057633 244.2489258 239.719016 221.5638761 259.2019287 231.3864836 187.6654065 176.2149409 168.2490842 175.2588701 168.4893686 154.1250731 156.6818584 167.9669694 166.0168305 163.47154 182.856651 232.0254509 179.8636143 174.5103995 191.0778481 170.07104 167.6774116 187.2887258 180.4972166 185.549896 198.7872605 160.9677046 201.2482594 184.3894752 192.8314975 187.7191592 186.7741603 170.5669017 175.8878758 150.6566876 152.8057445 148.2786177 150.0857824 266.9243154 252.8383765 266.5582549 256.4637915 236.2969358 255.8839377 246.6401794 224.6543648 244.7338804 234.265299 256.5271036 184.0846169 177.8587513 214.5115353 191.9241192 192.0084971 176.7921423 182.8002005 179.9368389 162.8331503 192.553501 198.5198418 264.6012446 212.3244968 199.8149747 210.0114846 210.9630903 194.6425917 198.1395057 175.5946265 178.1221045 195.5528217 173.4623378 293.2588919 227.9716517 220.5443198 213.5753073 210.165443 185.9331264 159.1877112 143.2666889 145.8154004 143.2895038 156.9514997 275.8870653 237.2127302 210.9854011 210.0127319 240.4200504 169.7981649 190.2192003 175.4219512 204.4761506 217.1109668 227.1012122 220.4798067 212.8323653 195.9497021 189.9310115 204.0284364 180.7579912 177.8594355 193.9542589 204.2722588 182.8734696 202.2965623 210.1880682 216.955188 207.2177177 210.2265518 186.0901442 203.4412234 170.0936059 184.3787458 161.3171707 179.5344824 177.3005328 269.8215496 218.0740888 188.3533539 194.8265518 243.3021867 185.8416335 144.73314 166.1327357 167.9445538 163.9876778 161.5164559 270.7488342 214.5124214 221.4300115 216.969598 222.9534166 201.0732165 198.2864529 198.1112153 191.4451824 173.9042273 181.0928553 242.5303166 198.9700811 199.7649099 191.6046645 191.7115587 204.2085382 185.5936386 193.401836 188.9479045 175.6015809 192.6880827 229.3111408 222.9933044 208.5522948 195.2703304 198.7705606 196.0162178 176.7682822 184.3233647 182.5215337 175.3073622 673.6588748 296.554553 198.3667105 200.884512 202.4881515 187.0868373 192.3966334 165.2785575 186.1801324 198.6385708 177.6163471 182.1041818 198.6234078 198.8229917 178.5627801 167.7596824 171.9797033 190.4385869 150.5787766 162.3645015 162.4965996 147.3832551 162.8217935 296.934312 248.5836179 236.3172251 251.4755196 223.9024176 217.1607825 218.792354 230.3198769 231.3411962 195.8363015 219.9135411 243.6035044 221.5224077 217.2837348 203.5767349 182.2240091 204.9816647 180.236018 205.0864045 175.1468053 229.0052593 213.7500155 254.3884352 219.2332242 229.5169258 220.2459087 223.5137797 191.7322836 205.4374352 214.7050101 226.0680866 224.4379882 217.2735177 263.2836563 219.9105867 218.0813591 214.4290588 218.0604538 220.7787004 179.8172159 209.0354888 202.9422293 211.0081679 193.0840993 156.4708245 154.0376875 156.7922629 160.2555878 154.3273246 148.9013064 139.9520584 139.0326762 145.2135408 134.3077335 122.5173466 149.9100771 161.1344217 154.3490363 163.7157939 160.6240239 147.2732179 148.4127876 145.1414071 140.8040743 135.2065566 143.2268648 258.5914376 173.2843505 171.4141971 174.4456352 169.5535371 158.561894 140.0886214 147.2474305 153.5029889 156.2225706 159.989798 252.4799803 164.8486723 140.3217665 161.922524 162.6675856 146.907505 143.0426701 145.2857793 121.5535992 169.4650075 259.5872107 248.6025456 244.5266088 257.3391526 222.6724669 243.100957 231.583832 204.4380392 219.7777986 229.2208562 165.430132 242.1244631 259.747835 254.0923752 262.2296203 252.6203645 261.3378792 229.3760178 232.3177393 234.0024658 223.8913708 232.9877109 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name aminoadipic Lysine METABOLITES_END #END