#METABOLOMICS WORKBENCH sean_harshman_20181008_113044_mwtab.txt DATATRACK_ID:1533 STUDY_ID:ST001070 ANALYSIS_ID:AN001752 PROJECT_ID:PR000715 VERSION 1 CREATED_ON October 10, 2018, 9:37 am #PROJECT PR:PROJECT_TITLE The proteomic and metabolomic characterization of exercise-induced sweat PR:PROJECT_SUMMARY Proteomic and Metabolomic analysis of exercise-induced sweat to evaluate analyte PR:PROJECT_SUMMARY correlation with human performance parameters. PR:INSTITUTE UES Inc, Air Force Research Laboratory PR:LAST_NAME Harshman PR:FIRST_NAME Sean PR:ADDRESS 2510 Fifth Street, Area B, Building 840, WPAFB, OH 45433 PR:EMAIL sean.harshman.ctr@us.af.mil PR:PHONE 937-938-3785 #STUDY ST:STUDY_TITLE The global proteomic characterization of exercise-induced sweat - (part II) ST:STUDY_SUMMARY The global proteomic characterization of exercise-induced sweat to evaluate the ST:STUDY_SUMMARY sweat protein content. ST:INSTITUTE UES Inc, Air Force Research Laboratory ST:LAST_NAME Harshman ST:FIRST_NAME Sean ST:ADDRESS 2510 Fifth Street, Area B, Building 840, WPAFB, OH 45433 ST:EMAIL sean.harshman.ctr@us.af.mil ST:PHONE 937-938-3785 #SUBJECT SU:SUBJECT_TYPE Human SU:SUBJECT_SPECIES Homo sapiens SU:TAXONOMY_ID 9606 #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS - Subject_#16_InSol Treadmill Speed:Hard SUBJECT_SAMPLE_FACTORS - Subject_#17_InSol Treadmill Speed:Low SUBJECT_SAMPLE_FACTORS - Subject_#19_InSol Treadmill Speed:Medium SUBJECT_SAMPLE_FACTORS - Subject_#20_InSol Treadmill Speed:Low SUBJECT_SAMPLE_FACTORS - Subject_#21_InSol Treadmill Speed:Low SUBJECT_SAMPLE_FACTORS - Subject_#22_InSol Treadmill Speed:Low SUBJECT_SAMPLE_FACTORS - Subject_#23_InSol Treadmill Speed:Medium #COLLECTION CO:COLLECTION_SUMMARY Sweat collected from the forearms of participants. CO:SAMPLE_TYPE Sweat #TREATMENT TR:TREATMENT_SUMMARY Sweat induced by exercise on a treadmill. #SAMPLEPREP SP:SAMPLEPREP_SUMMARY Sweat samples were collected, pooled, and frozen on liquid nitrogen. Following, SP:SAMPLEPREP_SUMMARY frozen samples were lyophilized and frozen until analysis. #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE Reversed phase CH:INSTRUMENT_NAME Thermo Dionex Ultimate 3000 RS CH:COLUMN_NAME Easy-Spray PepMap 50µm x 150µm, 100Å, 2µm #ANALYSIS AN:ANALYSIS_TYPE MS #MS MS:MS_COMMENTS - MS:INSTRUMENT_NAME Thermo LTQ XL MS:INSTRUMENT_TYPE Single quadrupole MS:MS_TYPE ESI MS:ION_MODE POSITIVE #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS Spectral Counts MS_METABOLITE_DATA_START Samples Subject_#16_InSol Subject_#17_InSol Subject_#19_InSol Subject_#20_InSol Subject_#21_InSol Subject_#22_InSol Subject_#23_InSol Factors Treadmill Speed:Hard Treadmill Speed:Low Treadmill Speed:Medium Treadmill Speed:Low Treadmill Speed:Low Treadmill Speed:Low Treadmill Speed:Medium Keratin; type I cytoskeletal 10 5 142 0 1 0 0 0 Serum albumin 0 0 0 96 0 7 0 Dermcidin 319 235 239 190 141 221 194 Keratin; type II cytoskeletal 1 0 83 0 9 0 0 0 Keratin; type II cytoskeletal 2 epidermal 0 72 0 1 0 0 0 Prolactin-inducible protein 82 64 35 65 19 27 39 "Serum albumin, Bovine" 3 3 10 28 0 1 0 Zinc-alpha-2-glycoprotein 28 24 17 27 5 13 4 Keratin; type I cytoskeletal 14 0 15 0 0 0 0 0 Secretoglobin family 1D member 2 18 13 3 10 1 6 0 Keratin; type II cytoskeletal 6C 0 12 0 0 0 0 0 Keratin; type I cytoskeletal 16 0 12 0 0 0 0 0 Ig kappa chain C region 0 0 0 14 0 3 0 Clusterin 5 7 3 0 0 2 0 Keratin; type I cytoskeletal 9 0 8 0 0 0 0 0 Desmoglein-1 0 6 2 0 0 3 0 Mucin-like protein 1 3 0 14 0 3 3 0 Calcium-transporting ATPase type 2C member 2 4 12 7 6 0 0 0 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name UniProt ID Keratin; type I cytoskeletal 10 P13645 Serum albumin P02768 Dermcidin P81605 Keratin; type II cytoskeletal 1 P04264 Keratin; type II cytoskeletal 2 epidermal P35908 Prolactin-inducible protein P12273 "Serum albumin, Bovine" P02769 Zinc-alpha-2-glycoprotein P25311 Keratin; type I cytoskeletal 14 P02533 Secretoglobin family 1D member 2 95969 Keratin; type II cytoskeletal 6C P48668 Keratin; type I cytoskeletal 16 P08779 Ig kappa chain C region P01834 Clusterin P10909 Keratin; type I cytoskeletal 9 P35527 Desmoglein-1 Q02413 Mucin-like protein 1 Q96DR8 Calcium-transporting ATPase type 2C member 2 O75185 METABOLITES_END #END