#METABOLOMICS WORKBENCH epannkuk_20190227_104940 DATATRACK_ID:1653 STUDY_ID:ST001146 ANALYSIS_ID:AN001891 PROJECT_ID:PR000766 VERSION 1 CREATED_ON March 12, 2019, 10:01 am #PROJECT PR:PROJECT_TITLE Nonhuman primate (NHP) radiation metabolomics PR:PROJECT_SUMMARY Here, we use global metabolomics to differentiate temporal effects (1 – 60 d) PR:PROJECT_SUMMARY found in nonhuman primate (NHP) urine small molecule signatures after a 4 Gy PR:PROJECT_SUMMARY total body irradiation. PR:INSTITUTE Georgetown University PR:LAST_NAME Pannkuk PR:FIRST_NAME Evan PR:ADDRESS 3970 Reservoir Rd, NW New Research Build, washington dc, District of Columbia, PR:ADDRESS 20057, USA PR:EMAIL elp44@georgetown.edu PR:PHONE 2026875650 #STUDY ST:STUDY_TITLE Global Nonhuman primate (NHP) urine metabolomics ST:STUDY_TYPE Nonhuman primate metabolomics ST:STUDY_SUMMARY Here, we use global metabolomics to differentiate temporal effects (1 – 60 d) ST:STUDY_SUMMARY found in nonhuman primate (NHP) urine small molecule signatures after a 4 Gy ST:STUDY_SUMMARY total body irradiation. ST:INSTITUTE Georgetown University ST:LAST_NAME Pannkuk ST:FIRST_NAME Evan ST:ADDRESS 3970 Reservoir Rd, NW New Research Build, washington dc, District of Columbia, ST:ADDRESS 20057, USA ST:EMAIL elp44@georgetown.edu ST:PHONE 2026875650 #SUBJECT SU:SUBJECT_TYPE Mammal SU:SUBJECT_SPECIES Macaca mulatta SU:TAXONOMY_ID 9544 #FACTORS #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS - POS_04 Time:5d SUBJECT_SAMPLE_FACTORS - POS_05 Time:28d SUBJECT_SAMPLE_FACTORS - POS_06 Time:pre SUBJECT_SAMPLE_FACTORS - POS_07 Time:7d SUBJECT_SAMPLE_FACTORS - POS_08 Time:7d SUBJECT_SAMPLE_FACTORS - POS_09 Time:pre SUBJECT_SAMPLE_FACTORS - POS_10 Time:pre SUBJECT_SAMPLE_FACTORS - POS_11 Time:21d SUBJECT_SAMPLE_FACTORS - POS_12 Time:60d SUBJECT_SAMPLE_FACTORS - POS_13 Time:1d SUBJECT_SAMPLE_FACTORS - POS_17 Time:3d SUBJECT_SAMPLE_FACTORS - POS_18 Time:28d SUBJECT_SAMPLE_FACTORS - POS_19 Time:7d SUBJECT_SAMPLE_FACTORS - POS_20 Time:28d SUBJECT_SAMPLE_FACTORS - POS_21 Time:1d SUBJECT_SAMPLE_FACTORS - POS_22 Time:7d SUBJECT_SAMPLE_FACTORS - POS_23 Time:5d SUBJECT_SAMPLE_FACTORS - POS_24 Time:pre SUBJECT_SAMPLE_FACTORS - POS_25 Time:5d SUBJECT_SAMPLE_FACTORS - POS_26 Time:21d SUBJECT_SAMPLE_FACTORS - POS_30 Time:pre SUBJECT_SAMPLE_FACTORS - POS_31 Time:28d SUBJECT_SAMPLE_FACTORS - POS_32 Time:1d SUBJECT_SAMPLE_FACTORS - POS_33 Time:3d SUBJECT_SAMPLE_FACTORS - POS_34 Time:3d SUBJECT_SAMPLE_FACTORS - POS_35 Time:7d SUBJECT_SAMPLE_FACTORS - POS_36 Time:60d SUBJECT_SAMPLE_FACTORS - POS_37 Time:21d SUBJECT_SAMPLE_FACTORS - POS_38 Time:15d SUBJECT_SAMPLE_FACTORS - POS_39 Time:pre SUBJECT_SAMPLE_FACTORS - POS_43 Time:pre SUBJECT_SAMPLE_FACTORS - POS_44 Time:60d SUBJECT_SAMPLE_FACTORS - POS_45 Time:15d SUBJECT_SAMPLE_FACTORS - POS_46 Time:1d SUBJECT_SAMPLE_FACTORS - POS_47 Time:pre SUBJECT_SAMPLE_FACTORS - POS_48 Time:21d SUBJECT_SAMPLE_FACTORS - POS_49 Time:pre SUBJECT_SAMPLE_FACTORS - POS_50 Time:28d SUBJECT_SAMPLE_FACTORS - POS_51 Time:pre SUBJECT_SAMPLE_FACTORS - POS_52 Time:60d SUBJECT_SAMPLE_FACTORS - POS_56 Time:pre SUBJECT_SAMPLE_FACTORS - POS_57 Time:15d SUBJECT_SAMPLE_FACTORS - POS_58 Time:1d SUBJECT_SAMPLE_FACTORS - POS_59 Time:15d SUBJECT_SAMPLE_FACTORS - POS_60 Time:15d SUBJECT_SAMPLE_FACTORS - POS_61 Time:5d SUBJECT_SAMPLE_FACTORS - POS_62 Time:pre SUBJECT_SAMPLE_FACTORS - POS_63 Time:15d SUBJECT_SAMPLE_FACTORS - POS_64 Time:pre SUBJECT_SAMPLE_FACTORS - POS_65 Time:1d SUBJECT_SAMPLE_FACTORS - POS_69 Time:60d SUBJECT_SAMPLE_FACTORS - POS_70 Time:1d SUBJECT_SAMPLE_FACTORS - POS_71 Time:1d SUBJECT_SAMPLE_FACTORS - POS_72 Time:60d SUBJECT_SAMPLE_FACTORS - POS_73 Time:7d SUBJECT_SAMPLE_FACTORS - POS_74 Time:28d SUBJECT_SAMPLE_FACTORS - POS_75 Time:7d SUBJECT_SAMPLE_FACTORS - POS_76 Time:pre SUBJECT_SAMPLE_FACTORS - POS_77 Time:3d SUBJECT_SAMPLE_FACTORS - POS_78 Time:21d SUBJECT_SAMPLE_FACTORS - POS_82 Time:3d SUBJECT_SAMPLE_FACTORS - POS_83 Time:28d SUBJECT_SAMPLE_FACTORS - POS_84 Time:7d SUBJECT_SAMPLE_FACTORS - POS_85 Time:7d SUBJECT_SAMPLE_FACTORS - POS_86 Time:21d SUBJECT_SAMPLE_FACTORS - POS_87 Time:3d SUBJECT_SAMPLE_FACTORS - POS_88 Time:5d SUBJECT_SAMPLE_FACTORS - POS_89 Time:60d SUBJECT_SAMPLE_FACTORS - POS_90 Time:5d SUBJECT_SAMPLE_FACTORS - POS_91 Time:60d SUBJECT_SAMPLE_FACTORS - POS_95 Time:1d SUBJECT_SAMPLE_FACTORS - POS_96 Time:5d SUBJECT_SAMPLE_FACTORS - POS_97 Time:21d SUBJECT_SAMPLE_FACTORS - POS_98 Time:3d SUBJECT_SAMPLE_FACTORS - POS_99 Time:15d SUBJECT_SAMPLE_FACTORS - POS_100 Time:60d SUBJECT_SAMPLE_FACTORS - POS_101 Time:15d SUBJECT_SAMPLE_FACTORS - POS_102 Time:3d SUBJECT_SAMPLE_FACTORS - POS_103 Time:28d SUBJECT_SAMPLE_FACTORS - POS_104 Time:15d SUBJECT_SAMPLE_FACTORS - POS_108 Time:28d SUBJECT_SAMPLE_FACTORS - POS_109 Time:21d SUBJECT_SAMPLE_FACTORS - POS_110 Time:21d SUBJECT_SAMPLE_FACTORS - POS_111 Time:21d SUBJECT_SAMPLE_FACTORS - POS_112 Time:5d SUBJECT_SAMPLE_FACTORS - POS_113 Time:pre SUBJECT_SAMPLE_FACTORS - POS_114 Time:5d SUBJECT_SAMPLE_FACTORS - POS_115 Time:28d SUBJECT_SAMPLE_FACTORS - POS_116 Time:5d SUBJECT_SAMPLE_FACTORS - POS_117 Time:3d SUBJECT_SAMPLE_FACTORS - POS_121 Time:7d SUBJECT_SAMPLE_FACTORS - POS_122 Time:15d SUBJECT_SAMPLE_FACTORS - POS_123 Time:pre SUBJECT_SAMPLE_FACTORS - POS_124 Time:pre SUBJECT_SAMPLE_FACTORS - POS_125 Time:pre SUBJECT_SAMPLE_FACTORS - POS_126 Time:3d SUBJECT_SAMPLE_FACTORS - POS_127 Time:60d SUBJECT_SAMPLE_FACTORS - POS_128 Time:1d SUBJECT_SAMPLE_FACTORS - POS_129 Time:pre SUBJECT_SAMPLE_FACTORS - POS_130 Time:pre #COLLECTION CO:COLLECTION_SUMMARY urine (20 μl) was deproteinated with 80 μl 50% cold acetonitrile with internal CO:COLLECTION_SUMMARY standards (2 μM debrisoquine, 176.1188 [M+H]+; 30 μM 4-nitrobenzoic acid, CO:COLLECTION_SUMMARY 166.0140 [M-H]-), vortexed, placed on ice for 10 min, and centrifuged for 10 min CO:COLLECTION_SUMMARY (10,000 x g, 4°C) CO:SAMPLE_TYPE Urine #TREATMENT TR:TREATMENT_SUMMARY 4 Gy at 0.6 Gy/min with a 60Co γ source #SAMPLEPREP SP:SAMPLEPREP_SUMMARY urine (20 μl) was deproteinated with 80 μl 50% cold acetonitrile with internal SP:SAMPLEPREP_SUMMARY standards (2 μM debrisoquine, 176.1188 [M+H]+; 30 μM 4-nitrobenzoic acid, SP:SAMPLEPREP_SUMMARY 166.0140 [M-H]-), vortexed, placed on ice for 10 min, and centrifuged for 10 min SP:SAMPLEPREP_SUMMARY (10,000 x g, 4°C) #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE Reversed phase CH:INSTRUMENT_NAME Waters Acquity CH:COLUMN_NAME Waters Acquity BEH C18 (50 x 2.1mm, 1.7 um) #ANALYSIS AN:ANALYSIS_TYPE MS #MS MS:INSTRUMENT_NAME Waters Synapt G2 QTOF MS:INSTRUMENT_TYPE QTOF MS:MS_TYPE ESI MS:ION_MODE UNSPECIFIED MS:MS_COMMENTS None MS:MS_RESULTS_FILE ST001146_AN001891_Results.txt UNITS:peak area Has m/z:Yes Has RT:Yes RT units:Minutes #END