#METABOLOMICS WORKBENCH epannkuk_20190228_060422_mwtab.txt DATATRACK_ID:1657 STUDY_ID:ST001147 ANALYSIS_ID:AN001892 PROJECT_ID:PR000766 VERSION 1 CREATED_ON March 12, 2019, 11:29 am #PROJECT PR:PROJECT_TITLE NHP radiation metabolomics PR:PROJECT_SUMMARY Here, we use global metabolomics to differentiate temporal effects (1 – 60 d) PR:PROJECT_SUMMARY found in nonhuman primate (NHP) serum small molecule signatures after a 4 Gy PR:PROJECT_SUMMARY total body irradiation. PR:INSTITUTE Georgetown University PR:LAST_NAME Pannkuk PR:FIRST_NAME Evan PR:ADDRESS 3970 Reservoir Rd, NW New Research Build, washington dc, District of Columbia, PR:ADDRESS 20057, USA PR:EMAIL elp44@georgetown.edu PR:PHONE 2026875650 #STUDY ST:STUDY_TITLE Global NHP serum metabolomics (part-IV) ST:STUDY_SUMMARY Here, we use global metabolomics to differentiate temporal effects (1 – 60 d) ST:STUDY_SUMMARY found in nonhuman primate (NHP) serum small molecule signatures after a 4 Gy ST:STUDY_SUMMARY total body irradiation. ST:INSTITUTE Georgetown University ST:LAST_NAME Pannkuk ST:FIRST_NAME Evan ST:ADDRESS 3970 Reservoir Rd, NW New Research Build, washington dc, District of Columbia, ST:ADDRESS 20057, USA ST:EMAIL elp44@georgetown.edu ST:PHONE 2026875650 #SUBJECT SU:SUBJECT_TYPE Mammal SU:SUBJECT_SPECIES Macaca mulatta SU:TAXONOMY_ID 9544 #FACTORS #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS - POS_04 Time:5d SUBJECT_SAMPLE_FACTORS - POS_05 Time:28d SUBJECT_SAMPLE_FACTORS - POS_06 Time:Pre SUBJECT_SAMPLE_FACTORS - POS_07 Time:7d SUBJECT_SAMPLE_FACTORS - POS_08 Time:7d SUBJECT_SAMPLE_FACTORS - POS_10 Time:Pre SUBJECT_SAMPLE_FACTORS - POS_11 Time:Pre SUBJECT_SAMPLE_FACTORS - POS_12 Time:21d SUBJECT_SAMPLE_FACTORS - POS_13 Time:60d SUBJECT_SAMPLE_FACTORS - POS_14 Time:1d SUBJECT_SAMPLE_FACTORS - POS_20 Time:3d SUBJECT_SAMPLE_FACTORS - POS_21 Time:28d SUBJECT_SAMPLE_FACTORS - POS_22 Time:7d SUBJECT_SAMPLE_FACTORS - POS_23 Time:28d SUBJECT_SAMPLE_FACTORS - POS_24 Time:1d SUBJECT_SAMPLE_FACTORS - POS_26 Time:7d SUBJECT_SAMPLE_FACTORS - POS_27 Time:5d SUBJECT_SAMPLE_FACTORS - POS_28 Time:Pre SUBJECT_SAMPLE_FACTORS - POS_29 Time:5d SUBJECT_SAMPLE_FACTORS - POS_30 Time:21d SUBJECT_SAMPLE_FACTORS - POS_36 Time:Pre SUBJECT_SAMPLE_FACTORS - POS_37 Time:28d SUBJECT_SAMPLE_FACTORS - POS_38 Time:1d SUBJECT_SAMPLE_FACTORS - POS_39 Time:3d SUBJECT_SAMPLE_FACTORS - POS_40 Time:3d SUBJECT_SAMPLE_FACTORS - POS_42 Time:7d SUBJECT_SAMPLE_FACTORS - POS_43 Time:60d SUBJECT_SAMPLE_FACTORS - POS_44 Time:21d SUBJECT_SAMPLE_FACTORS - POS_45 Time:15d SUBJECT_SAMPLE_FACTORS - POS_46 Time:Pre SUBJECT_SAMPLE_FACTORS - POS_52 Time:Pre SUBJECT_SAMPLE_FACTORS - POS_53 Time:60d SUBJECT_SAMPLE_FACTORS - POS_54 Time:15d SUBJECT_SAMPLE_FACTORS - POS_55 Time:1d SUBJECT_SAMPLE_FACTORS - POS_56 Time:Pre SUBJECT_SAMPLE_FACTORS - POS_58 Time:21d SUBJECT_SAMPLE_FACTORS - POS_59 Time:Pre SUBJECT_SAMPLE_FACTORS - POS_60 Time:28d SUBJECT_SAMPLE_FACTORS - POS_61 Time:Pre SUBJECT_SAMPLE_FACTORS - POS_62 Time:60d SUBJECT_SAMPLE_FACTORS - POS_68 Time:Pre SUBJECT_SAMPLE_FACTORS - POS_69 Time:15d SUBJECT_SAMPLE_FACTORS - POS_70 Time:1d SUBJECT_SAMPLE_FACTORS - POS_71 Time:15d SUBJECT_SAMPLE_FACTORS - POS_72 Time:15d SUBJECT_SAMPLE_FACTORS - POS_74 Time:5d SUBJECT_SAMPLE_FACTORS - POS_75 Time:Pre SUBJECT_SAMPLE_FACTORS - POS_76 Time:15d SUBJECT_SAMPLE_FACTORS - POS_77 Time:Pre SUBJECT_SAMPLE_FACTORS - POS_78 Time:1d SUBJECT_SAMPLE_FACTORS - POS_84 Time:60d SUBJECT_SAMPLE_FACTORS - POS_85 Time:1d SUBJECT_SAMPLE_FACTORS - POS_86 Time:1d SUBJECT_SAMPLE_FACTORS - POS_87 Time:60d SUBJECT_SAMPLE_FACTORS - POS_88 Time:7d SUBJECT_SAMPLE_FACTORS - POS_90 Time:28d SUBJECT_SAMPLE_FACTORS - POS_91 Time:7d SUBJECT_SAMPLE_FACTORS - POS_92 Time:Pre SUBJECT_SAMPLE_FACTORS - POS_93 Time:3d SUBJECT_SAMPLE_FACTORS - POS_94 Time:21d SUBJECT_SAMPLE_FACTORS - POS_100 Time:3d SUBJECT_SAMPLE_FACTORS - POS_101 Time:28d SUBJECT_SAMPLE_FACTORS - POS_102 Time:7d SUBJECT_SAMPLE_FACTORS - POS_103 Time:7d SUBJECT_SAMPLE_FACTORS - POS_104 Time:21d SUBJECT_SAMPLE_FACTORS - POS_106 Time:3d SUBJECT_SAMPLE_FACTORS - POS_107 Time:5d SUBJECT_SAMPLE_FACTORS - POS_108 Time:60d SUBJECT_SAMPLE_FACTORS - POS_109 Time:5d SUBJECT_SAMPLE_FACTORS - POS_110 Time:60d SUBJECT_SAMPLE_FACTORS - POS_116 Time:1d SUBJECT_SAMPLE_FACTORS - POS_117 Time:5d SUBJECT_SAMPLE_FACTORS - POS_118 Time:21d SUBJECT_SAMPLE_FACTORS - POS_119 Time:3d SUBJECT_SAMPLE_FACTORS - POS_120 Time:15d SUBJECT_SAMPLE_FACTORS - POS_122 Time:60d SUBJECT_SAMPLE_FACTORS - POS_123 Time:15d SUBJECT_SAMPLE_FACTORS - POS_124 Time:3d SUBJECT_SAMPLE_FACTORS - POS_125 Time:28d SUBJECT_SAMPLE_FACTORS - POS_126 Time:15d SUBJECT_SAMPLE_FACTORS - POS_132 Time:28d SUBJECT_SAMPLE_FACTORS - POS_133 Time:21d SUBJECT_SAMPLE_FACTORS - POS_134 Time:21d SUBJECT_SAMPLE_FACTORS - POS_135 Time:21d SUBJECT_SAMPLE_FACTORS - POS_136 Time:5d SUBJECT_SAMPLE_FACTORS - POS_138 Time:Pre SUBJECT_SAMPLE_FACTORS - POS_139 Time:5d SUBJECT_SAMPLE_FACTORS - POS_140 Time:28d SUBJECT_SAMPLE_FACTORS - POS_141 Time:5d SUBJECT_SAMPLE_FACTORS - POS_142 Time:3d SUBJECT_SAMPLE_FACTORS - POS_148 Time:7d SUBJECT_SAMPLE_FACTORS - POS_149 Time:15d SUBJECT_SAMPLE_FACTORS - POS_150 Time:Pre SUBJECT_SAMPLE_FACTORS - POS_151 Time:Pre SUBJECT_SAMPLE_FACTORS - POS_152 Time:Pre SUBJECT_SAMPLE_FACTORS - POS_154 Time:3d SUBJECT_SAMPLE_FACTORS - POS_155 Time:60d SUBJECT_SAMPLE_FACTORS - POS_156 Time:1d SUBJECT_SAMPLE_FACTORS - POS_157 Time:Pre SUBJECT_SAMPLE_FACTORS - POS_158 Time:Pre #COLLECTION CO:COLLECTION_SUMMARY None CO:SAMPLE_TYPE Blood (serum) #TREATMENT TR:TREATMENT_SUMMARY 4 Gy at 0.6 Gy/min with a 60Co γ source #SAMPLEPREP SP:SAMPLEPREP_SUMMARY Serum (5 μl) was deproteinated with 195 μl 66% cold acetonitrile with internal SP:SAMPLEPREP_SUMMARY standards (2 μM debrisoquine, 176.1188 [M+H]+; 30 μM 4-nitrobenzoic acid, SP:SAMPLEPREP_SUMMARY 166.0140 [M-H]-), vortexed, placed on ice for 10 min, and centrifuged for 10 min SP:SAMPLEPREP_SUMMARY (10,000 x g, 4°C) SP:PROCESSING_STORAGE_CONDITIONS On ice SP:EXTRACT_STORAGE On ice #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE Reversed phase CH:INSTRUMENT_NAME Waters Acquity CH:COLUMN_NAME Waters Acquity BEH C18 (50 x 2.1mm, 1.7 um) #ANALYSIS AN:ANALYSIS_TYPE MS #MS MS:INSTRUMENT_NAME Waters Synapt G2 QTOF MS:INSTRUMENT_TYPE QTOF MS:MS_TYPE ESI MS:ION_MODE UNSPECIFIED MS:MS_COMMENTS None MS:MS_RESULTS_FILE ST001147_AN001892_Results.txt UNITS:peak area Has m/z:Yes Has RT:Yes RT units:Minutes #END