#METABOLOMICS WORKBENCH myaish_20190318_070818 DATATRACK_ID:1677 STUDY_ID:ST001155 ANALYSIS_ID:AN001912 PROJECT_ID:PR000774 VERSION 1 CREATED_ON March 22, 2019, 11:27 am #PROJECT PR:PROJECT_TITLE The Effect of Silicon on Salinity Tolerance and the Associated Metabolomics PR:PROJECT_TITLE Profile Changes in Date Palm (Phoenix dactylifera L.) PR:PROJECT_TYPE Plant Metabolites PR:PROJECT_SUMMARY Silicon has a promising role in the growth and salinity tolerance in plants. PR:PROJECT_SUMMARY While the results obtained from the current study showed that silicon enhanced PR:PROJECT_SUMMARY growth in date palm seedlings, the mechanism behind this observation was also PR:PROJECT_SUMMARY investigated by studying changes occurred in metabolomic profiles triggered by PR:PROJECT_SUMMARY silicon under salinity. The global metabolomic analysis using PR:PROJECT_SUMMARY liquid-chromatography-mass-spectrometry revealed the presence of a number PR:PROJECT_SUMMARY significantly (p ≤ 0.05) accumulated metabolites in leaves and roots when PR:PROJECT_SUMMARY plants were irrigated with silicon and grown under control and salinity PR:PROJECT_SUMMARY conditions. PR:INSTITUTE Sultan Qaboos University PR:DEPARTMENT Biology PR:LABORATORY Mahmoud Yaish PR:LAST_NAME Yaish PR:FIRST_NAME Mahmoud PR:ADDRESS Department of Biology, College of Science, Sultan Qaboos University PR:EMAIL mack.yaish@gmail.com PR:PHONE 968 24146823 PR:FUNDING_SOURCE The research council of Oman, Oman #STUDY ST:STUDY_TITLE The Effect of Silicon on Salinity Tolerance and the Associated Metabolomics ST:STUDY_TITLE Profile Changes in Date Palm (part-I) ST:STUDY_SUMMARY Silicon has a promising role in the growth and salinity tolerance in plants. ST:STUDY_SUMMARY While the results obtained from the current study showed that silicon enhanced ST:STUDY_SUMMARY growth in date palm seedlings, the mechanism behind this observation was also ST:STUDY_SUMMARY investigated by studying changes occurred in metabolomic profiles triggered by ST:STUDY_SUMMARY silicon under salinity. The global metabolomic analysis using ST:STUDY_SUMMARY liquid-chromatography-mass-spectrometry revealed the presence of a number ST:STUDY_SUMMARY significantly (p ≤ 0.05) accumulated metabolites in leaves and roots when ST:STUDY_SUMMARY plants were irrigated with silicon and grown under control and salinity ST:STUDY_SUMMARY conditions. ST:INSTITUTE Sultan Qaboos University ST:DEPARTMENT Biology ST:LAST_NAME Yaish ST:FIRST_NAME Mahmoud ST:ADDRESS Sultan Qaboos University, Department of Biology, College of Science ST:EMAIL mack.yaish@gmail.com ST:PHONE 968 24146823 #SUBJECT SU:SUBJECT_TYPE Plant SU:SUBJECT_SPECIES Phoenix dactylifera SU:TAXONOMY_ID 42345 #FACTORS #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS SiR SiR3 Treatment:Siicon-root SUBJECT_SAMPLE_FACTORS SiR SiR2 Treatment:Siicon-root SUBJECT_SAMPLE_FACTORS SiR SiR1 Treatment:Siicon-root SUBJECT_SAMPLE_FACTORS SiL SiL3 Treatment:silicon-leaf SUBJECT_SAMPLE_FACTORS SiL SiL2 Treatment:silicon-leaf SUBJECT_SAMPLE_FACTORS SiL SiL1 Treatment:silicon-leaf SUBJECT_SAMPLE_FACTORS NSiR NSiR3 Treatment:NaCl-Root-Si SUBJECT_SAMPLE_FACTORS NSiR NSiR2 Treatment:NaCl-Root-Si SUBJECT_SAMPLE_FACTORS NSiR NSiR1 Treatment:NaCl-Root-Si SUBJECT_SAMPLE_FACTORS NSiL NSiL3 Treatment:NaCl-Leaf-Si SUBJECT_SAMPLE_FACTORS NSiL NSiL2 Treatment:NaCl-Leaf-Si SUBJECT_SAMPLE_FACTORS NSiL NSiL1 Treatment:NaCl-Leaf-Si SUBJECT_SAMPLE_FACTORS NR NR3 Treatment:NaCl-Root SUBJECT_SAMPLE_FACTORS NR NR2 Treatment:NaCl-Root SUBJECT_SAMPLE_FACTORS NR NR1 Treatment:NaCl-Root SUBJECT_SAMPLE_FACTORS NL NL3 Treatment:NaCl-Leaf SUBJECT_SAMPLE_FACTORS NL NL2 Treatment:NaCl-Leaf SUBJECT_SAMPLE_FACTORS NL NL1 Treatment:NaCl-Leaf SUBJECT_SAMPLE_FACTORS CR CR3 Treatment:Contro-leaf SUBJECT_SAMPLE_FACTORS CR CR2 Treatment:Contro-leaf SUBJECT_SAMPLE_FACTORS CR CR1 Treatment:Contro-leaf SUBJECT_SAMPLE_FACTORS CL CL3 Treatment:Control-leaf SUBJECT_SAMPLE_FACTORS CL CL2 Treatment:Control-leaf SUBJECT_SAMPLE_FACTORS CL CL1 Treatment:Control-leaf #COLLECTION CO:COLLECTION_SUMMARY Leaf and root tissues were collected from date palm seedlings after treatment CO:COLLECTION_SUMMARY with NaCl and/or silicon, flash frozen with liquid nitrogen, and freeze dried. CO:SAMPLE_TYPE Plant #TREATMENT TR:TREATMENT_SUMMARY Once the radicle of the seeds had emerged, they were then transferred to potting TR:TREATMENT_SUMMARY compost and maintained at 32C/30C with a photoperiod of 16/8 h in the growth TR:TREATMENT_SUMMARY chamber. The seedlings were then divided into four sets based on their TR:TREATMENT_SUMMARY treatments and were irrigated either with tap water with an electrical TR:TREATMENT_SUMMARY conductivity of 50 mS/m, considered as a control treatment (control), 300 mM TR:TREATMENT_SUMMARY NaCl without additional silicon for salt stress (NaCl), 5 mM Na2SiO3 (+Si) for TR:TREATMENT_SUMMARY silicon treatment or 5 mM of sodium silicate (Na2SiO3) along with 300 mM NaCl TR:TREATMENT_SUMMARY (NaCl+Si) for salt stress and silicon treatment. The NaCl and silicon treatments TR:TREATMENT_SUMMARY were administered on a weekly basis, as irrigation. #SAMPLEPREP SP:SAMPLEPREP_SUMMARY A total of 24 samples were analyzed using LC-MS by HILIC chromatography on a 0.5 SP:SAMPLEPREP_SUMMARY x 150 mm Zic-pHILIC column using 10 mM NH4HCO3 in water (A) and 10 mM NH4HCO3 in SP:SAMPLEPREP_SUMMARY 95% acetonitrile (B) as solvents and by RPLC chromatography using the same SP:SAMPLEPREP_SUMMARY solvents and a 0.5 x 100 mm PLRPS column. The Samples were extracted at a tissue SP:SAMPLEPREP_SUMMARY concentration of 100 mg/mL (leaves) or 50 mg/mL (roots) in 80% methanol by SP:SAMPLEPREP_SUMMARY vortexing for 10 minutes. The solids were collected by centrifugation, and then SP:SAMPLEPREP_SUMMARY the supernatant was filtered through a 0.8 μm spin filter. Samples were SP:SAMPLEPREP_SUMMARY injected onto the LC-MS and data was acquired in polarity switching mode with SP:SAMPLEPREP_SUMMARY data dependent acquisition of MS/MS spectra. #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE Reversed phase CH:INSTRUMENT_NAME Thermo Q-Exactive CH:COLUMN_NAME 0.5 x 100 mm PLRPS column #ANALYSIS AN:ANALYSIS_TYPE MS AN:LABORATORY_NAME Donald Danforth Plant Science Center, Saint Louis, MO 63132, USA AN:OPERATOR_NAME Bradley S. Evans #MS MS:INSTRUMENT_NAME Thermo Q Exactive Orbitrap MS:INSTRUMENT_TYPE Orbitrap MS:MS_TYPE ESI MS:ION_MODE UNSPECIFIED MS:MS_COMMENTS Samples were injected onto the LC-MS and data was acquired in polarity switching MS:MS_COMMENTS mode with data dependent acquisition of MS/MS spectra. The ion mode is MS:MS_COMMENTS polarity-switching, which means it scans in positive and negative ion mode MS:MS_COMMENTS during the sample run, switching between the two modes about every 20 MS:MS_COMMENTS milliseconds. MS:MS_RESULTS_FILE ST001155_AN001912_Results.txt UNITS:log10(peak area) Has m/z:No Has RT:Yes RT units:Minutes #END