#METABOLOMICS WORKBENCH myaish_20190318_122642 DATATRACK_ID:1679 STUDY_ID:ST001156 ANALYSIS_ID:AN001913 PROJECT_ID:PR000775 VERSION 1 CREATED_ON March 22, 2019, 11:55 am #PROJECT PR:PROJECT_TITLE The Effect of Silicon on Salinity Tolerance and the Associated Metabolomics PR:PROJECT_TITLE Profile Changes in Date Palm PR:PROJECT_SUMMARY Since metabolites are the net products of the central dogma of cellular biology, PR:PROJECT_SUMMARY this study was aimed to decipher salinity tolerance depends on the information PR:PROJECT_SUMMARY encoded by the metabolomic profiles of the salt tolerant ‘Umsila’ and PR:PROJECT_SUMMARY susceptible ‘Zabad’ cultivars when grown under salinity. Changes in the PR:PROJECT_SUMMARY metabolomic profiles of the leaf and root tissues were determined using PR:PROJECT_SUMMARY hydrophilic interaction liquid chromatography (HILIC) and reverse phase liquid PR:PROJECT_SUMMARY (RPLC) mass spectrometery. PR:INSTITUTE Sultan Qaboos University, College of Science PR:DEPARTMENT Biology PR:LABORATORY Plant Molecular Biology PR:LAST_NAME Yaish PR:FIRST_NAME Mahmoud PR:ADDRESS Department of Biology, College of Science, Sultan Qaboos University, PR:EMAIL mack.yaish@gmail.com PR:PHONE 96824146823 #STUDY ST:STUDY_TITLE Comparative Metabolomic Profiling of Two Contrasting Date Palm Genotypes under ST:STUDY_TITLE Salinity Stress (part-II) ST:STUDY_SUMMARY Since metabolites are the net products of the central dogma of cellular biology, ST:STUDY_SUMMARY this study was aimed to decipher salinity tolerance depends on the information ST:STUDY_SUMMARY encoded by the metabolomic profiles of the salt tolerant ‘Umsila’ and ST:STUDY_SUMMARY susceptible ‘Zabad’ cultivars when grown under salinity. Changes in the ST:STUDY_SUMMARY metabolomic profiles of the leaf and root tissues were determined using ST:STUDY_SUMMARY hydrophilic interaction liquid chromatography (HILIC) and reverse phase liquid ST:STUDY_SUMMARY (RPLC) mass spectrometry. ST:INSTITUTE Sultan Qaboos University ST:LAST_NAME Yaish ST:FIRST_NAME Mahmoud ST:ADDRESS Department of Biology, College of Science, Sultan Qaboos University ST:EMAIL mack.yaish@gmail.com ST:PHONE 968 24146823 #SUBJECT SU:SUBJECT_TYPE Plant SU:SUBJECT_SPECIES Phoenix dactylifera SU:TAXONOMY_ID 42345 #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS Control MCLU-1 Factor:control leaf Umsila SUBJECT_SAMPLE_FACTORS Control MCLU-2 Factor:control leaf Umsila SUBJECT_SAMPLE_FACTORS Control MCLU-3 Factor:control leaf Umsila SUBJECT_SAMPLE_FACTORS Control MCLZ-1 Factor:control leaf Zabad SUBJECT_SAMPLE_FACTORS Control MCLZ-2 Factor:control leaf Zabad SUBJECT_SAMPLE_FACTORS Control MCLZ-3 Factor:control leaf Zabad SUBJECT_SAMPLE_FACTORS Control MCRU-1 Factor:Control root Umsila SUBJECT_SAMPLE_FACTORS Control MCRU-2 Factor:Control root Umsila SUBJECT_SAMPLE_FACTORS Control MCRU-3 Factor:Control root Umsila SUBJECT_SAMPLE_FACTORS Control MCRZ-1 Factor:Control root Zabad SUBJECT_SAMPLE_FACTORS Control MCRZ-2 Factor:Control root Zabad SUBJECT_SAMPLE_FACTORS Control MCRZ-3 Factor:Control root Zabad SUBJECT_SAMPLE_FACTORS Salinity MTLU-1 Factor:Treated leaf Umsila SUBJECT_SAMPLE_FACTORS Salinity MTLU-2 Factor:Treated leaf Umsila SUBJECT_SAMPLE_FACTORS Salinity MTLU-3 Factor:Treated leaf Umsila SUBJECT_SAMPLE_FACTORS Salinity MTLZ-1 Factor:Treated leaf Zabad SUBJECT_SAMPLE_FACTORS Salinity MTLZ-2 Factor:Treated leaf Zabad SUBJECT_SAMPLE_FACTORS Salinity MTLZ-3 Factor:Treated leaf Zabad SUBJECT_SAMPLE_FACTORS Salinity MTRU-1 Factor:Treated root Umsila SUBJECT_SAMPLE_FACTORS Salinity MTRU-2 Factor:Treated root Umsila SUBJECT_SAMPLE_FACTORS Salinity MTRU-3 Factor:Treated root Umsila SUBJECT_SAMPLE_FACTORS Salinity MTRZ-1 Factor:Treated root Zabad SUBJECT_SAMPLE_FACTORS Salinity MTRZ-2 Factor:Treated root Zabad SUBJECT_SAMPLE_FACTORS Salinity MTRZ-3 Factor:Treated root Zabad #COLLECTION CO:COLLECTION_SUMMARY Leaf and root tissues of Umsila and Zabad cultivars were collected after CO:COLLECTION_SUMMARY irrigation either with water or NaCl solution. CO:SAMPLE_TYPE Plant #TREATMENT TR:TREATMENT_SUMMARY Seedlings were either irrigated with water or NaCl solution for about 2 months. #SAMPLEPREP SP:SAMPLEPREP_SUMMARY Metabolomics and basic data analysis were carried out for service charges at SP:SAMPLEPREP_SUMMARY proteomics and mass spectrometry facility, Donald Danforth Plant Science Centre, SP:SAMPLEPREP_SUMMARY Sant Louis, USA, using liquid chromatography mass spectrometry. Briefly, a total SP:SAMPLEPREP_SUMMARY of 24 samples were analyzed for polar metabolites using hydrophilic interaction SP:SAMPLEPREP_SUMMARY liquid chromatography (HILIC) and nonpolar metabolites using reverse phase SP:SAMPLEPREP_SUMMARY liquid chromatography (RPLC). The Samples were extracted in 80% methanol at a SP:SAMPLEPREP_SUMMARY tissue concentration of 100 mg/mL by a vortex for 10 minutes. The solids were SP:SAMPLEPREP_SUMMARY collected by centrifugation, and then the supernatant was filtered through a 0.8 SP:SAMPLEPREP_SUMMARY μm spin filter. The extracts were injected into the LC-MS and the data was SP:SAMPLEPREP_SUMMARY acquired in polarity switching mode with data dependent acquisition of MS/MS SP:SAMPLEPREP_SUMMARY spectra. #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE HILIC CH:INSTRUMENT_NAME Thermo Q-Exactive CH:COLUMN_NAME chromatography on a 0.5 x 150 mm #ANALYSIS AN:ANALYSIS_TYPE MS AN:LABORATORY_NAME Donald Danforth Plant Science Center, Saint Louis, MO 63132, USA AN:OPERATOR_NAME Bradley S. Evans #MS MS:INSTRUMENT_NAME Thermo Q Exactive Orbitrap MS:INSTRUMENT_TYPE Orbitrap MS:MS_TYPE ESI MS:ION_MODE UNSPECIFIED MS:MS_COMMENTS The ion mode is polarity-switching, which means it scans in positive and MS:MS_COMMENTS negative ion mode during the sample run, switching between the two modes about MS:MS_COMMENTS every 20 milliseconds. MS:MS_RESULTS_FILE ST001156_AN001913_Results.txt UNITS:log10(peak area) Has m/z:No Has RT:Yes RT units:Minutes #END