#METABOLOMICS WORKBENCH araskind_20190724_074339 DATATRACK_ID:1782 STUDY_ID:ST001229 ANALYSIS_ID:AN002042 VERSION 1 CREATED_ON 03-03-2022 #PROJECT PR:PROJECT_TITLE Aquamin and Prevention of Colon Cancer PR:PROJECT_TYPE MS analysis PR:PROJECT_SUMMARY Effects of calcium and multi-mineral supplementation on gut microbes and PR:PROJECT_SUMMARY microbially-derived metabolites in patients at risk for colon cancer PR:INSTITUTE University of Michigan PR:DEPARTMENT Pathology PR:LABORATORY Aslam Lab PR:LAST_NAME Aslam PR:FIRST_NAME Muhammad Nadeem PR:ADDRESS Ann Arbor, MI PR:EMAIL mnaslam@umich.edu PR:PHONE 734-936-1897 PR:DOI http://dx.doi.org/10.21228/M8F69D #STUDY ST:STUDY_TITLE Aquamin and Prevention of Colon Cancer (part-V) ST:STUDY_TYPE MS analysis ST:STUDY_SUMMARY We propose to evaluate microbial and metabolic profiles in baseline and endpoint ST:STUDY_SUMMARY colonic mucosal, fecal, and serum samples from human patients at risk for CRC ST:STUDY_SUMMARY and enrolled in a 90-day phase I clinical trial. Patients will receive daily ST:STUDY_SUMMARY supplementation with calcium alone, a calcium-rich multimineral (Aquamin?), or ST:STUDY_SUMMARY placebo (maltodextrin) (n=10 per group). We hypothesize that dietary ST:STUDY_SUMMARY supplementation will correlate with CRC-protective metabolic profiles and that ST:STUDY_SUMMARY multimineral supplementation will generate more favorable profiles than calcium ST:STUDY_SUMMARY supplementation alone. ST:INSTITUTE University of Michigan ST:DEPARTMENT Biomedical Research Core Facilities ST:LABORATORY Metabolomics core ST:LAST_NAME Kachman ST:FIRST_NAME Maureen ST:ADDRESS Ann Arbor, MI ST:EMAIL mkachman@med.umich.edu ST:PHONE 734-232-0842 ST:SUBMIT_DATE 2019-07-24 #SUBJECT SU:SUBJECT_TYPE Human SU:SUBJECT_SPECIES Homo sapiens SU:TAXONOMY_ID 9606 #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS SU0021803 S00031763 Sample Source:Feces | Time Point:Baseline Sample type=Sample; 10001 Visit 2 Colon NA#2=10001 Visit 2 Stool NA#7 SUBJECT_SAMPLE_FACTORS SU0021804 S00031764 Sample Source:Feces | Time Point:Baseline Sample type=Sample; 10001 Visit 2 Colon NA#2=10002 Visit 2 Stool NA#7 SUBJECT_SAMPLE_FACTORS SU0021805 S00031765 Sample Source:Feces | Time Point:Baseline Sample type=Sample; 10001 Visit 2 Colon NA#2=10003 Visit 2 Stool NA#7 SUBJECT_SAMPLE_FACTORS SU0021806 S00031766 Sample Source:Feces | Time Point:Baseline Sample type=Sample; 10001 Visit 2 Colon NA#2=10004 Visit 2 Stool NA#7 SUBJECT_SAMPLE_FACTORS SU0021807 S00031767 Sample Source:Feces | Time Point:Baseline Sample type=Sample; 10001 Visit 2 Colon NA#2=10005 Visit 2 Stool NA#7 SUBJECT_SAMPLE_FACTORS SU0021808 S00031768 Sample Source:Feces | Time Point:Baseline Sample type=Sample; 10001 Visit 2 Colon NA#2=10006 Visit 2 Stool NA#7 SUBJECT_SAMPLE_FACTORS SU0021809 S00031769 Sample Source:Feces | Time Point:Baseline Sample type=Sample; 10001 Visit 2 Colon NA#2=10007 Visit 2 Stool NA#7 SUBJECT_SAMPLE_FACTORS SU0021810 S00031770 Sample Source:Feces | Time Point:Baseline Sample type=Sample; 10001 Visit 2 Colon NA#2=10008 Visit 2 Stool NA#7 SUBJECT_SAMPLE_FACTORS SU0021811 S00031771 Sample Source:Feces | Time Point:Baseline Sample type=Sample; 10001 Visit 2 Colon NA#2=10009 Visit 2 Stool NA#7 SUBJECT_SAMPLE_FACTORS SU0021812 S00031772 Sample Source:Feces | Time Point:Baseline Sample type=Sample; 10001 Visit 2 Colon NA#2=10010 Visit 2 Stool NA#7 SUBJECT_SAMPLE_FACTORS SU0021813 S00031773 Sample Source:Feces | Time Point:Baseline Sample type=Sample; 10001 Visit 2 Colon NA#2=10011 Visit 2 Stool NA#7 SUBJECT_SAMPLE_FACTORS SU0021814 S00031774 Sample Source:Feces | Time Point:Baseline Sample type=Sample; 10001 Visit 2 Colon NA#2=10012 Visit 2 Stool NA#7 SUBJECT_SAMPLE_FACTORS SU0021815 S00031775 Sample Source:Feces | Time Point:Baseline Sample type=Sample; 10001 Visit 2 Colon NA#2=10013 Visit 2 Stool NA#7 SUBJECT_SAMPLE_FACTORS SU0021816 S00031776 Sample Source:Feces | Time Point:Baseline Sample type=Sample; 10001 Visit 2 Colon NA#2=10014 Visit 2 Stool NA#7 SUBJECT_SAMPLE_FACTORS SU0021817 S00031777 Sample Source:Feces | Time Point:Baseline Sample type=Sample; 10001 Visit 2 Colon NA#2=10015 Visit 2 Stool NA#7 SUBJECT_SAMPLE_FACTORS SU0021818 S00031778 Sample Source:Feces | Time Point:Baseline Sample type=Sample; 10001 Visit 2 Colon NA#2=10016 Visit 2 Stool NA#7 SUBJECT_SAMPLE_FACTORS SU0021819 S00031779 Sample Source:Feces | Time Point:Baseline Sample type=Sample; 10001 Visit 2 Colon NA#2=10017 Visit 2 Stool NA#7 SUBJECT_SAMPLE_FACTORS SU0021820 S00031780 Sample Source:Feces | Time Point:Baseline Sample type=Sample; 10001 Visit 2 Colon NA#2=10018 Visit 2 Stool NA#7 SUBJECT_SAMPLE_FACTORS SU0021803 S00031781 Sample Source:Feces | Time Point:Day 90 Sample type=Sample; 10001 Visit 2 Colon NA#2=10001 Visit 3 Stool NA#7 SUBJECT_SAMPLE_FACTORS SU0021804 S00031782 Sample Source:Feces | Time Point:Day 90 Sample type=Sample; 10001 Visit 2 Colon NA#2=10002 Visit 3 Stool NA#7 SUBJECT_SAMPLE_FACTORS SU0021805 S00031783 Sample Source:Feces | Time Point:Day 90 Sample type=Sample; 10001 Visit 2 Colon NA#2=10003 Visit 3 Stool NA#7 SUBJECT_SAMPLE_FACTORS SU0021806 S00031784 Sample Source:Feces | Time Point:Day 90 Sample type=Sample; 10001 Visit 2 Colon NA#2=10004 Visit 3 Stool NA#7 SUBJECT_SAMPLE_FACTORS SU0021807 S00031785 Sample Source:Feces | Time Point:Day 90 Sample type=Sample; 10001 Visit 2 Colon NA#2=10005 Visit 3 Stool NA#7 SUBJECT_SAMPLE_FACTORS SU0021808 S00031786 Sample Source:Feces | Time Point:Day 90 Sample type=Sample; 10001 Visit 2 Colon NA#2=10006 Visit 3 Stool NA#7 SUBJECT_SAMPLE_FACTORS SU0021809 S00031787 Sample Source:Feces | Time Point:Day 90 Sample type=Sample; 10001 Visit 2 Colon NA#2=10007 Visit 3 Stool NA#7 SUBJECT_SAMPLE_FACTORS SU0021810 S00031788 Sample Source:Feces | Time Point:Day 90 Sample type=Sample; 10001 Visit 2 Colon NA#2=10008 Visit 3 Stool NA#7 SUBJECT_SAMPLE_FACTORS SU0021811 S00031789 Sample Source:Feces | Time Point:Day 90 Sample type=Sample; 10001 Visit 2 Colon NA#2=10009 Visit 3 Stool NA#7 SUBJECT_SAMPLE_FACTORS SU0021812 S00031790 Sample Source:Feces | Time Point:Day 90 Sample type=Sample; 10001 Visit 2 Colon NA#2=10010 Visit 3 Stool NA#7 SUBJECT_SAMPLE_FACTORS SU0021813 S00031791 Sample Source:Feces | Time Point:Day 90 Sample type=Sample; 10001 Visit 2 Colon NA#2=10011 Visit 3 Stool NA#7 SUBJECT_SAMPLE_FACTORS SU0021814 S00031792 Sample Source:Feces | Time Point:Day 90 Sample type=Sample; 10001 Visit 2 Colon NA#2=10012 Visit 3 Stool NA#7 SUBJECT_SAMPLE_FACTORS SU0021815 S00031793 Sample Source:Feces | Time Point:Day 90 Sample type=Sample; 10001 Visit 2 Colon NA#2=10013 Visit 3 Stool NA#7 SUBJECT_SAMPLE_FACTORS SU0021816 S00031794 Sample Source:Feces | Time Point:Day 90 Sample type=Sample; 10001 Visit 2 Colon NA#2=10014 Visit 3 Stool NA#7 SUBJECT_SAMPLE_FACTORS SU0021817 S00031795 Sample Source:Feces | Time Point:Day 90 Sample type=Sample; 10001 Visit 2 Colon NA#2=10015 Visit 3 Stool NA#7 SUBJECT_SAMPLE_FACTORS SU0021818 S00031796 Sample Source:Feces | Time Point:Day 90 Sample type=Sample; 10001 Visit 2 Colon NA#2=10016 Visit 3 Stool NA#7 SUBJECT_SAMPLE_FACTORS SU0021819 S00031797 Sample Source:Feces | Time Point:Day 90 Sample type=Sample; 10001 Visit 2 Colon NA#2=10017 Visit 3 Stool NA#7 SUBJECT_SAMPLE_FACTORS SU0021820 S00031798 Sample Source:Feces | Time Point:Day 90 Sample type=Sample; 10001 Visit 2 Colon NA#2=10018 Visit 3 Stool NA#7 SUBJECT_SAMPLE_FACTORS SU0021803 S00031799 Sample Source:Serum | Time Point:Baseline Sample type=Sample; 10001 Visit 2 Colon NA#2=10001 Visit 2 Serum SUBJECT_SAMPLE_FACTORS SU0021804 S00031800 Sample Source:Serum | Time Point:Baseline Sample type=Sample; 10001 Visit 2 Colon NA#2=10002 Visit 2 Serum SUBJECT_SAMPLE_FACTORS SU0021805 S00031801 Sample Source:Serum | Time Point:Baseline Sample type=Sample; 10001 Visit 2 Colon NA#2=10003 Visit 2 Serum SUBJECT_SAMPLE_FACTORS SU0021806 S00031802 Sample Source:Serum | Time Point:Baseline Sample type=Sample; 10001 Visit 2 Colon NA#2=10004 Visit 2 Serum SUBJECT_SAMPLE_FACTORS SU0021807 S00031803 Sample Source:Serum | Time Point:Baseline Sample type=Sample; 10001 Visit 2 Colon NA#2=10005 Visit 2 Serum SUBJECT_SAMPLE_FACTORS SU0021808 S00031804 Sample Source:Serum | Time Point:Baseline Sample type=Sample; 10001 Visit 2 Colon NA#2=10006 Visit 2 Serum SUBJECT_SAMPLE_FACTORS SU0021809 S00031805 Sample Source:Serum | Time Point:Baseline Sample type=Sample; 10001 Visit 2 Colon NA#2=10007 Visit 2 Serum SUBJECT_SAMPLE_FACTORS SU0021810 S00031806 Sample Source:Serum | Time Point:Baseline Sample type=Sample; 10001 Visit 2 Colon NA#2=10008 Visit 2 Serum SUBJECT_SAMPLE_FACTORS SU0021811 S00031807 Sample Source:Serum | Time Point:Baseline Sample type=Sample; 10001 Visit 2 Colon NA#2=10009 Visit 2 Serum SUBJECT_SAMPLE_FACTORS SU0021812 S00031808 Sample Source:Serum | Time Point:Baseline Sample type=Sample; 10001 Visit 2 Colon NA#2=10010 Visit 2 Serum SUBJECT_SAMPLE_FACTORS SU0021813 S00031809 Sample Source:Serum | Time Point:Baseline Sample type=Sample; 10001 Visit 2 Colon NA#2=10011 Visit 2 Serum SUBJECT_SAMPLE_FACTORS SU0021814 S00031810 Sample Source:Serum | Time Point:Baseline Sample type=Sample; 10001 Visit 2 Colon NA#2=10012 Visit 2 Serum SUBJECT_SAMPLE_FACTORS SU0021815 S00031811 Sample Source:Serum | Time Point:Baseline Sample type=Sample; 10001 Visit 2 Colon NA#2=10013 Visit 2 Serum SUBJECT_SAMPLE_FACTORS SU0021816 S00031812 Sample Source:Serum | Time Point:Baseline Sample type=Sample; 10001 Visit 2 Colon NA#2=10014 Visit 2 Serum SUBJECT_SAMPLE_FACTORS SU0021817 S00031813 Sample Source:Serum | Time Point:Baseline Sample type=Sample; 10001 Visit 2 Colon NA#2=10015 Visit 2 Serum SUBJECT_SAMPLE_FACTORS SU0021818 S00031814 Sample Source:Serum | Time Point:Baseline Sample type=Sample; 10001 Visit 2 Colon NA#2=10016 Visit 2 Serum SUBJECT_SAMPLE_FACTORS SU0021819 S00031815 Sample Source:Serum | Time Point:Baseline Sample type=Sample; 10001 Visit 2 Colon NA#2=10017 Visit 2 Serum SUBJECT_SAMPLE_FACTORS SU0021820 S00031816 Sample Source:Serum | Time Point:Baseline Sample type=Sample; 10001 Visit 2 Colon NA#2=10018 Visit 2 Serum SUBJECT_SAMPLE_FACTORS SU0021803 S00031817 Sample Source:Serum | Time Point:Day 90 Sample type=Sample; 10001 Visit 2 Colon NA#2=10001 Visit 3 Serum SUBJECT_SAMPLE_FACTORS SU0021804 S00031818 Sample Source:Serum | Time Point:Day 90 Sample type=Sample; 10001 Visit 2 Colon NA#2=10002 Visit 3 Serum SUBJECT_SAMPLE_FACTORS SU0021805 S00031819 Sample Source:Serum | Time Point:Day 90 Sample type=Sample; 10001 Visit 2 Colon NA#2=10003 Visit 3 Serum SUBJECT_SAMPLE_FACTORS SU0021806 S00031820 Sample Source:Serum | Time Point:Day 90 Sample type=Sample; 10001 Visit 2 Colon NA#2=10004 Visit 3 Serum SUBJECT_SAMPLE_FACTORS SU0021807 S00031821 Sample Source:Serum | Time Point:Day 90 Sample type=Sample; 10001 Visit 2 Colon NA#2=10005 Visit 3 Serum SUBJECT_SAMPLE_FACTORS SU0021808 S00031822 Sample Source:Serum | Time Point:Day 90 Sample type=Sample; 10001 Visit 2 Colon NA#2=10006 Visit 3 Serum SUBJECT_SAMPLE_FACTORS SU0021809 S00031823 Sample Source:Serum | Time Point:Day 90 Sample type=Sample; 10001 Visit 2 Colon NA#2=10007 Visit 3 Serum SUBJECT_SAMPLE_FACTORS SU0021810 S00031824 Sample Source:Serum | Time Point:Day 90 Sample type=Sample; 10001 Visit 2 Colon NA#2=10008 Visit 3 Serum SUBJECT_SAMPLE_FACTORS SU0021811 S00031825 Sample Source:Serum | Time Point:Day 90 Sample type=Sample; 10001 Visit 2 Colon NA#2=10009 Visit 3 Serum SUBJECT_SAMPLE_FACTORS SU0021812 S00031826 Sample Source:Serum | Time Point:Day 90 Sample type=Sample; 10001 Visit 2 Colon NA#2=10010 Visit 3 Serum SUBJECT_SAMPLE_FACTORS SU0021813 S00031827 Sample Source:Serum | Time Point:Day 90 Sample type=Sample; 10001 Visit 2 Colon NA#2=10011 Visit 3 Serum SUBJECT_SAMPLE_FACTORS SU0021814 S00031828 Sample Source:Serum | Time Point:Day 90 Sample type=Sample; 10001 Visit 2 Colon NA#2=10012 Visit 3 Serum SUBJECT_SAMPLE_FACTORS SU0021815 S00031829 Sample Source:Serum | Time Point:Day 90 Sample type=Sample; 10001 Visit 2 Colon NA#2=10013 Visit 3 Serum SUBJECT_SAMPLE_FACTORS SU0021816 S00031830 Sample Source:Serum | Time Point:Day 90 Sample type=Sample; 10001 Visit 2 Colon NA#2=10014 Visit 3 Serum SUBJECT_SAMPLE_FACTORS SU0021817 S00031831 Sample Source:Serum | Time Point:Day 90 Sample type=Sample; 10001 Visit 2 Colon NA#2=10015 Visit 3 Serum SUBJECT_SAMPLE_FACTORS SU0021818 S00031832 Sample Source:Serum | Time Point:Day 90 Sample type=Sample; 10001 Visit 2 Colon NA#2=10016 Visit 3 Serum SUBJECT_SAMPLE_FACTORS SU0021819 S00031833 Sample Source:Serum | Time Point:Day 90 Sample type=Sample; 10001 Visit 2 Colon NA#2=10017 Visit 3 Serum SUBJECT_SAMPLE_FACTORS SU0021820 S00031834 Sample Source:Serum | Time Point:Day 90 Sample type=Sample; 10001 Visit 2 Colon NA#2=10018 Visit 3 Serum SUBJECT_SAMPLE_FACTORS SU0021803 S00031727 Sample Source:Tissues | Time Point:Baseline Sample type=Sample; 10001 Visit 2 Colon NA#2=10001 Visit 2 Colon NA#2 SUBJECT_SAMPLE_FACTORS SU0021804 S00031728 Sample Source:Tissues | Time Point:Baseline Sample type=Sample; 10001 Visit 2 Colon NA#2=10002 Visit 2 Colon NA#2 SUBJECT_SAMPLE_FACTORS SU0021805 S00031729 Sample Source:Tissues | Time Point:Baseline Sample type=Sample; 10001 Visit 2 Colon NA#2=10003 Visit 2 Colon NA#2 SUBJECT_SAMPLE_FACTORS SU0021806 S00031730 Sample Source:Tissues | Time Point:Baseline Sample type=Sample; 10001 Visit 2 Colon NA#2=10004 Visit 2 Colon NA#2 SUBJECT_SAMPLE_FACTORS SU0021807 S00031731 Sample Source:Tissues | Time Point:Baseline Sample type=Sample; 10001 Visit 2 Colon NA#2=10005 Visit 2 Colon NA#2 SUBJECT_SAMPLE_FACTORS SU0021808 S00031732 Sample Source:Tissues | Time Point:Baseline Sample type=Sample; 10001 Visit 2 Colon NA#2=10006 Visit 2 Colon NA#2 SUBJECT_SAMPLE_FACTORS SU0021809 S00031733 Sample Source:Tissues | Time Point:Baseline Sample type=Sample; 10001 Visit 2 Colon NA#2=10007 Visit 2 Colon NA#2 SUBJECT_SAMPLE_FACTORS SU0021810 S00031734 Sample Source:Tissues | Time Point:Baseline Sample type=Sample; 10001 Visit 2 Colon NA#2=10008 Visit 2 Colon NA#2 SUBJECT_SAMPLE_FACTORS SU0021811 S00031735 Sample Source:Tissues | Time Point:Baseline Sample type=Sample; 10001 Visit 2 Colon NA#2=10009 Visit 2 Colon NA#2 SUBJECT_SAMPLE_FACTORS SU0021812 S00031736 Sample Source:Tissues | Time Point:Baseline Sample type=Sample; 10001 Visit 2 Colon NA#2=10010 Visit 2 Colon NA#2 SUBJECT_SAMPLE_FACTORS SU0021813 S00031737 Sample Source:Tissues | Time Point:Baseline Sample type=Sample; 10001 Visit 2 Colon NA#2=10011 Visit 2 Colon NA#2 SUBJECT_SAMPLE_FACTORS SU0021814 S00031738 Sample Source:Tissues | Time Point:Baseline Sample type=Sample; 10001 Visit 2 Colon NA#2=10012 Visit 2 Colon NA#2 SUBJECT_SAMPLE_FACTORS SU0021815 S00031739 Sample Source:Tissues | Time Point:Baseline Sample type=Sample; 10001 Visit 2 Colon NA#2=10013 Visit 2 Colon NA#2 SUBJECT_SAMPLE_FACTORS SU0021816 S00031740 Sample Source:Tissues | Time Point:Baseline Sample type=Sample; 10001 Visit 2 Colon NA#2=10014 Visit 2 Colon NA#2 SUBJECT_SAMPLE_FACTORS SU0021817 S00031741 Sample Source:Tissues | Time Point:Baseline Sample type=Sample; 10001 Visit 2 Colon NA#2=10015 Visit 2 Colon NA#2 SUBJECT_SAMPLE_FACTORS SU0021818 S00031742 Sample Source:Tissues | Time Point:Baseline Sample type=Sample; 10001 Visit 2 Colon NA#2=10016 Visit 2 Colon NA#2 SUBJECT_SAMPLE_FACTORS SU0021819 S00031743 Sample Source:Tissues | Time Point:Baseline Sample type=Sample; 10001 Visit 2 Colon NA#2=10017 Visit 2 Colon NA#2 SUBJECT_SAMPLE_FACTORS SU0021820 S00031744 Sample Source:Tissues | Time Point:Baseline Sample type=Sample; 10001 Visit 2 Colon NA#2=10018 Visit 2 Colon NA#2 SUBJECT_SAMPLE_FACTORS SU0021803 S00031745 Sample Source:Tissues | Time Point:Day 90 Sample type=Sample; 10001 Visit 2 Colon NA#2=10001 Visit 3 Colon NA#2 SUBJECT_SAMPLE_FACTORS SU0021804 S00031746 Sample Source:Tissues | Time Point:Day 90 Sample type=Sample; 10001 Visit 2 Colon NA#2=10002 Visit 3 Colon NA#2 SUBJECT_SAMPLE_FACTORS SU0021805 S00031747 Sample Source:Tissues | Time Point:Day 90 Sample type=Sample; 10001 Visit 2 Colon NA#2=10003 Visit 3 Colon NA#2 SUBJECT_SAMPLE_FACTORS SU0021806 S00031748 Sample Source:Tissues | Time Point:Day 90 Sample type=Sample; 10001 Visit 2 Colon NA#2=10004 Visit 3 Colon NA#2 SUBJECT_SAMPLE_FACTORS SU0021807 S00031749 Sample Source:Tissues | Time Point:Day 90 Sample type=Sample; 10001 Visit 2 Colon NA#2=10005 Visit 3 Colon NA#2 SUBJECT_SAMPLE_FACTORS SU0021808 S00031750 Sample Source:Tissues | Time Point:Day 90 Sample type=Sample; 10001 Visit 2 Colon NA#2=10006 Visit 3 Colon NA#2 SUBJECT_SAMPLE_FACTORS SU0021809 S00031751 Sample Source:Tissues | Time Point:Day 90 Sample type=Sample; 10001 Visit 2 Colon NA#2=10007 Visit 3 Colon NA#2 SUBJECT_SAMPLE_FACTORS SU0021810 S00031752 Sample Source:Tissues | Time Point:Day 90 Sample type=Sample; 10001 Visit 2 Colon NA#2=10008 Visit 3 Colon NA#2 SUBJECT_SAMPLE_FACTORS SU0021811 S00031753 Sample Source:Tissues | Time Point:Day 90 Sample type=Sample; 10001 Visit 2 Colon NA#2=10009 Visit 3 Colon NA#2 SUBJECT_SAMPLE_FACTORS SU0021812 S00031754 Sample Source:Tissues | Time Point:Day 90 Sample type=Sample; 10001 Visit 2 Colon NA#2=10010 Visit 3 Colon NA#2 SUBJECT_SAMPLE_FACTORS SU0021813 S00031755 Sample Source:Tissues | Time Point:Day 90 Sample type=Sample; 10001 Visit 2 Colon NA#2=10011 Visit 3 Colon NA#2 SUBJECT_SAMPLE_FACTORS SU0021814 S00031756 Sample Source:Tissues | Time Point:Day 90 Sample type=Sample; 10001 Visit 2 Colon NA#2=10012 Visit 3 Colon NA#2 SUBJECT_SAMPLE_FACTORS SU0021815 S00031757 Sample Source:Tissues | Time Point:Day 90 Sample type=Sample; 10001 Visit 2 Colon NA#2=10013 Visit 3 Colon NA#2 SUBJECT_SAMPLE_FACTORS SU0021816 S00031758 Sample Source:Tissues | Time Point:Day 90 Sample type=Sample; 10001 Visit 2 Colon NA#2=10014 Visit 3 Colon NA#2 SUBJECT_SAMPLE_FACTORS SU0021817 S00031759 Sample Source:Tissues | Time Point:Day 90 Sample type=Sample; 10001 Visit 2 Colon NA#2=10015 Visit 3 Colon NA#2 SUBJECT_SAMPLE_FACTORS SU0021818 S00031760 Sample Source:Tissues | Time Point:Day 90 Sample type=Sample; 10001 Visit 2 Colon NA#2=10016 Visit 3 Colon NA#2 SUBJECT_SAMPLE_FACTORS SU0021819 S00031761 Sample Source:Tissues | Time Point:Day 90 Sample type=Sample; 10001 Visit 2 Colon NA#2=10017 Visit 3 Colon NA#2 SUBJECT_SAMPLE_FACTORS SU0021820 S00031762 Sample Source:Tissues | Time Point:Day 90 Sample type=Sample; 10001 Visit 2 Colon NA#2=10018 Visit 3 Colon NA#2 #COLLECTION CO:COLLECTION_SUMMARY Not available. CO:SAMPLE_TYPE Feces #TREATMENT TR:TREATMENT_SUMMARY Not available. #SAMPLEPREP SP:SAMPLEPREP_SUMMARY Profile of 28 Eicosanoids in plasma or tissue. Eicosanoids are extracted and SP:SAMPLEPREP_SUMMARY concentrated using solid phase extraction. The eluant is dried and re-suspended SP:SAMPLEPREP_SUMMARY for LCMS separation by RPLC and measurements by ESI- QQQ MRM methods. Analytes SP:SAMPLEPREP_SUMMARY are reported as uM or ug/mg (tissue), and CV’s are generally <20%. SP:SAMPLEPREP_PROTOCOL_FILENAME EX00759 _eicosanoid_protocol.pdf #CHROMATOGRAPHY CH:INSTRUMENT_NAME Agilent 1290 CH:COLUMN_NAME Phenomenex Luna-C18 CH:CHROMATOGRAPHY_TYPE Reversed phase #ANALYSIS AN:ANALYSIS_TYPE MS AN:ANALYSIS_PROTOCOL_FILE EX00759 - eicosanoid protocol.pdf AN:ACQUISITION_PARAMETERS_FILE Eis_LunaC18_HA64FA02_AC55_MRM_Halade_2Seg_B10-port1-6-a1-b1.m #MS MS:INSTRUMENT_NAME Agilent 6490 QQQ MS:INSTRUMENT_TYPE Triple quadrupole MS:MS_TYPE ESI MS:MS_COMMENTS NA MS:ION_MODE NEGATIVE #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS pmol/mg MS_METABOLITE_DATA_START Samples S00031727 S00031728 S00031729 S00031730 S00031731 S00031732 S00031733 S00031734 S00031735 S00031736 S00031737 S00031738 S00031739 S00031740 S00031741 S00031742 S00031743 S00031744 S00031745 S00031746 S00031747 S00031748 S00031749 S00031750 S00031751 S00031752 S00031753 S00031754 S00031755 S00031756 S00031757 S00031758 S00031759 S00031760 S00031761 S00031762 Factors Sample Source:Tissues | Time Point:Baseline Sample Source:Tissues | Time Point:Baseline Sample Source:Tissues | Time Point:Baseline Sample Source:Tissues | Time Point:Baseline Sample Source:Tissues | Time Point:Baseline Sample Source:Tissues | Time Point:Baseline Sample Source:Tissues | Time Point:Baseline Sample Source:Tissues | Time Point:Baseline Sample Source:Tissues | Time Point:Baseline Sample Source:Tissues | Time Point:Baseline Sample Source:Tissues | Time Point:Baseline Sample Source:Tissues | Time Point:Baseline Sample Source:Tissues | Time Point:Baseline Sample Source:Tissues | Time Point:Baseline Sample Source:Tissues | Time Point:Baseline Sample Source:Tissues | Time Point:Baseline Sample Source:Tissues | Time Point:Baseline Sample Source:Tissues | Time Point:Baseline Sample Source:Tissues | Time Point:Day 90 Sample Source:Tissues | Time Point:Day 90 Sample Source:Tissues | Time Point:Day 90 Sample Source:Tissues | Time Point:Day 90 Sample Source:Tissues | Time Point:Day 90 Sample Source:Tissues | Time Point:Day 90 Sample Source:Tissues | Time Point:Day 90 Sample Source:Tissues | Time Point:Day 90 Sample Source:Tissues | Time Point:Day 90 Sample Source:Tissues | Time Point:Day 90 Sample Source:Tissues | Time Point:Day 90 Sample Source:Tissues | Time Point:Day 90 Sample Source:Tissues | Time Point:Day 90 Sample Source:Tissues | Time Point:Day 90 Sample Source:Tissues | Time Point:Day 90 Sample Source:Tissues | Time Point:Day 90 Sample Source:Tissues | Time Point:Day 90 Sample Source:Tissues | Time Point:Day 90 11(S)-Hydroxy-5Z_8Z_12E_14Z-eicosatetraenoic acid 7.4250 20.4060 13.6060 26.0210 5.1340 35.8790 8.2070 8.6440 26.9380 23.7800 5.9690 7.3380 22.1540 27.4070 16.6260 21.3770 30.3490 10.9310 13.6750 26.9070 13.2210 25.5470 10.1090 12.5210 29.8240 19.0310 4.9460 33.6500 10.9140 18.9110 22.0480 12.4240 12.1090 28.0840 38.5200 17.1420 12-oxo-6E_8Z_10E_14Z-eicosatetraenoic acid 3.8490 27.1470 2.9530 17.2710 2.2920 14.0510 2.2290 1.8870 18.9550 13.1980 6.5540 8.1390 19.4940 18.0970 5.4530 36.9330 10.3690 9.4540 11.7960 14.9890 4.5280 21.3370 4.9130 6.2910 4.9280 15.4400 3.9080 57.5380 28.2540 31.1560 21.9360 6.2630 12.8900 17.5880 12.8630 20.6080 12(S)-Hydroxy-5Z_8Z_10E_14Z-eicosatetraenoic acid 4.1550 9.5230 4.6920 13.6680 1.8580 19.1740 3.9110 2.5780 15.1450 13.2380 4.0560 3.9490 9.8720 11.3510 6.5580 11.0400 20.2070 6.5200 7.8550 11.9680 5.9060 10.6590 2.5950 5.2590 15.7670 8.2890 1.4490 17.5290 6.3220 11.7730 10.5530 5.0340 4.3260 13.1770 20.1610 9.0090 13S-hydroxy-9Z_11E-octadecadienoic acid 125.9080 158.6760 76.8190 154.8720 21.2700 593.2550 77.2660 48.5980 277.5040 219.4260 30.1490 68.9370 141.5050 182.5050 63.5480 116.7120 106.1670 58.9620 130.8880 381.6570 161.0480 325.5680 35.2860 85.2200 189.2350 202.4800 31.2450 290.7590 100.3080 97.2610 267.7160 162.1190 68.2830 202.1590 201.5140 145.9070 15(S)-Hydroxy-65Z_8Z_11Z_13E-eicosatetraenoic acid 11.9620 42.6080 20.0220 52.9230 8.4450 133.1950 10.6240 7.8310 56.1980 50.2100 8.6780 19.4430 30.4520 35.0970 18.0900 33.0840 30.7350 23.6810 31.7570 42.5670 19.1600 84.7270 10.1070 24.3670 31.8400 37.6140 6.0470 93.0130 37.9000 45.2070 70.5700 20.6960 22.3580 45.1980 38.3510 57.4710 20-Hydroxy-5Z_8Z_11Z_14Z-eicosatetraenoic acid 0.2140 0.4470 0.9430 0.5700 0.3380 0.5770 0.2780 0.2150 0.0990 0.4150 0.0190 0.2200 0.4860 0.2490 0.2190 0.0740 0.4100 0.2750 0.1920 0.2480 0.1030 0.0650 0.1970 0.5290 0.1510 0.1960 0.0940 0.2020 0.2690 0.3210 0.1160 0.1180 0.3110 0.1600 0.3410 0.5780 5-oxo-6E_8Z_11Z_14Z-eicosatetraenoic acid 3.3280 27.1010 3.3560 18.1270 2.6110 13.6190 2.1010 1.8040 18.7650 14.0220 5.9990 8.3160 17.1360 18.9060 6.5950 34.6660 10.7410 9.0550 12.3060 15.8380 4.5390 20.8860 4.2480 6.0130 4.6120 17.4600 3.9480 58.0790 28.8770 32.4590 24.9240 7.1210 14.6490 18.0320 13.3350 22.6390 5(S)-Hydroxy-6E_8Z_11Z_14Z-eicosatetraenoic acid 11.9980 30.3930 15.7650 39.9490 6.7280 144.5190 7.2720 6.6870 60.0140 46.6660 8.8400 13.2310 36.0270 41.7240 17.7180 36.8390 31.2370 25.0660 26.5620 47.2230 16.3550 85.9090 11.3190 17.1510 31.1000 48.9050 5.0200 77.5170 34.7850 32.9310 78.5420 20.4660 19.6160 40.7340 50.4880 41.9730 6-Keto-Prostaglandin F1alpha 0.7020 10.4100 7.8200 0.7430 3.5510 7.8660 2.4170 7.1060 3.7160 1.8320 1.2900 0.4350 4.4020 7.1430 7.4310 7.8630 7.3560 0.6900 0.4070 10.3210 2.4110 0.4440 10.2490 5.7420 11.1970 1.9380 2.6770 4.6050 0.6800 0.4220 4.9230 7.4020 5.6770 4.2850 6.7370 0.5030 7S_16R_17S-trihydroxy-4Z_8E_10Z_12E_14E_19Z-docosahexaenoic acid 0.0690 0.0630 0.0960 0.1230 0.0510 0.1700 0.0610 0.0570 0.1930 0.0540 0.0270 0.3330 0.0520 0.0510 0.0800 0.0640 0.0530 0.0420 0.1010 0.1760 0.1230 0.2240 0.0670 0.1280 0.0230 0.0760 0.0410 0.1190 0.0610 0.1180 0.1710 0.0510 0.0640 0.0580 0.0480 0.0500 7S_8R_17S-trihydroxy-4Z_9E_11E_13Z_15E_19Z-docosahexaenoic acid 0.2100 0.1090 0.2960 0.1690 0.1280 0.1660 0.1580 0.0660 0.2800 0.0330 0.0190 0.8550 0.0760 0.1150 0.9120 0.0400 0.0850 0.0400 0.1480 0.3300 0.2080 0.0950 0.0560 0.2120 0.2830 0.1180 0.0770 0.1180 0.1040 0.1400 0.1470 0.1410 0.2750 0.0750 0.0420 0.0600 8(S)-Hydroxy-5ZE_9E_11Z_14Z-eicosatetraenoic acid 6.7300 17.3590 8.3900 26.3890 3.2400 41.7020 8.2130 4.1520 32.5230 25.4090 6.8570 7.2500 19.1650 21.0180 11.6230 22.1650 43.5390 12.5860 14.8190 25.9890 11.4610 21.2630 4.4630 8.8560 32.4000 17.3200 2.9840 33.0810 12.3830 18.7010 22.0210 9.9330 8.5600 24.9810 43.7250 17.1900 9(S)-Hydroxy-5Z_7E_11Z_14Z-eicosatetraenoic acid 11.8340 28.3100 11.7610 40.5340 5.5590 77.2050 11.2290 7.9250 59.4030 42.8580 11.4050 11.6930 32.4700 37.6730 20.0500 35.6200 62.7890 19.1950 25.0820 43.4750 19.1940 40.0310 7.4280 15.9200 49.0550 30.1590 4.6430 62.5330 18.8560 34.1970 41.4690 16.4050 13.3470 46.8830 62.5790 28.8960 Leukotriene B4 0.1520 0.9490 0.3200 0.9460 0.2060 5.2750 0.1470 0.0950 1.4840 2.5130 0.1580 0.7400 1.3210 1.0980 0.2810 0.9990 0.4970 0.6320 0.5990 1.0160 0.5070 3.1180 0.2080 0.4850 0.4200 1.0430 0.1410 2.9600 0.8840 1.3560 1.8540 0.4640 0.7210 1.3540 0.9240 1.4560 Progtaglandin F2a 0.6880 14.1900 4.4890 1.8360 2.1670 10.4110 8.4380 4.6040 4.8590 4.7270 2.6380 1.2440 2.5800 3.5280 5.3710 6.1170 3.1740 2.0190 0.7400 4.6380 3.0040 0.9640 2.3460 2.8640 3.9510 3.5200 5.4910 9.4340 0.9990 0.8040 3.0470 2.4580 5.5510 2.2070 4.4730 2.0010 Prostaglandin A2 0.0940 0.7330 1.7750 0.1540 0.1590 0.9610 0.2040 0.4810 0.5100 0.3070 0.1140 0.1370 0.2040 0.2430 0.5840 0.4160 0.2620 0.1170 0.1160 0.5130 0.3940 0.3050 0.2620 0.2650 0.4590 0.3370 0.0970 0.3480 0.1470 0.1810 0.4950 0.2250 0.3930 0.3360 0.5780 0.4580 Prostaglandin B2 0.0440 0.0720 0.1150 0.0390 0.0410 0.0780 0.0440 0.0650 0.0670 0.0530 0.0250 0.1280 0.0470 0.0460 0.0520 0.0320 0.0400 0.0380 0.0540 0.0760 0.0640 0.0600 0.0640 0.1080 0.0590 0.0990 0.0510 0.0470 0.0290 0.0730 0.0480 0.0350 0.0560 0.0440 0.0390 0.0350 Prostaglandin D2 1.0460 6.8870 4.5440 4.3250 1.3630 14.5030 0.8270 2.1820 7.1950 7.4930 1.6640 2.0410 3.6630 4.6690 2.8860 5.1640 2.6870 3.0790 4.1250 5.8820 2.1950 7.7740 1.7450 2.8520 2.8760 4.5460 2.1660 12.2430 5.0010 3.8220 5.7310 2.9470 5.4290 5.1040 4.8700 5.8540 Prostaglandin E2 1.3220 8.0280 4.6840 6.1240 1.5830 20.5450 0.5630 2.2780 9.2820 9.4750 2.6070 2.4950 4.3710 5.0160 2.7200 6.1590 2.9030 3.7420 5.3630 6.7480 2.6040 10.0690 1.5190 2.6150 3.2670 3.7930 2.3020 16.2620 5.5660 4.4940 7.8230 4.1490 7.3040 5.3020 5.1220 6.8910 Thromboxane B2 0.3260 0.6730 1.8070 0.1330 0.3650 1.1260 0.2700 0.3320 0.5660 0.3820 0.1040 0.8150 0.4310 0.2850 0.7290 0.3890 0.1060 0.0410 0.5300 0.5540 0.1010 0.8250 0.7860 1.6340 0.4690 0.6940 0.7210 0.0790 0.0100 0.5170 0.7550 0.3810 1.4870 0.3920 0.0680 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name pubchem_id inchi_key kegg_id other_id other_id_type ri ri_type moverz_quant 11(S)-Hydroxy-5Z,8Z,12E,14Z-eicosatetraenoic acid 5283152 12-oxo-6E,8Z,10E,14Z-eicosatetraenoic acid 5283162 12(S)-Hydroxy-5Z,8Z,10E,14Z-eicosatetraenoic acid 5283155 13S-hydroxy-9Z,11E-octadecadienoic acid 6443013 15(S)-Hydroxy-65Z,8Z,11Z,13E-eicosatetraenoic acid 5280724 20-Hydroxy-5Z,8Z,11Z,14Z-eicosatetraenoic acid 5283157 5-oxo-6E,8Z,11Z,14Z-eicosatetraenoic acid 5283159 5(S)-Hydroxy-6E,8Z,11Z,14Z-eicosatetraenoic acid 5280733 6-Keto-Prostaglandin F1alpha 5280888 7S,16R,17S-trihydroxy-4Z,8E,10Z,12E,14E,19Z-docosahexaenoic acid 11383310 7S,8R,17S-trihydroxy-4Z,9E,11E,13Z,15E,19Z-docosahexaenoic acid 44251266 8(S)-Hydroxy-5ZE,9E,11Z,14Z-eicosatetraenoic acid 5283154 9(S)-Hydroxy-5Z,7E,11Z,14Z-eicosatetraenoic acid 5283167 Leukotriene B4 5280492 Progtaglandin F2a 5280363 Prostaglandin A2 5280880 Prostaglandin B2 5280881 Prostaglandin D2 448457 Prostaglandin E2 5280360 Thromboxane B2 5283137 METABOLITES_END #END