#METABOLOMICS WORKBENCH ahmohamed_20200302_175121 DATATRACK_ID:1932 STUDY_ID:ST001323 ANALYSIS_ID:AN002200 PROJECT_ID:PR000900
VERSION             	1
CREATED_ON             	March 5, 2020, 9:58 pm
#PROJECT
PR:PROJECT_TITLE                 	Effect of high-fat diet on serum lipidome in mice
PR:PROJECT_SUMMARY               	We analyzed mouse serum samples from a mouse dietary intervention experiment.
PR:PROJECT_SUMMARY               	Briefly, C57BL/6 mice (n=44) were divided into 4 groups (n=11 per group) and fed
PR:PROJECT_SUMMARY               	High-fat diet (HFD), 1% deoxycholic acid (DCA) in drinking water, both, or left
PR:PROJECT_SUMMARY               	as control for 9 months. For quality control, 12 TQC samples and 2 blanks were
PR:PROJECT_SUMMARY               	also included in the analysis (total 58 samples and 6 groups). The two
PR:PROJECT_SUMMARY               	treatments were selected to demonstrate the ability of lipidomics to detect
PR:PROJECT_SUMMARY               	gross changes induced by HFD in the serum lipidome, as well as specific/minor
PR:PROJECT_SUMMARY               	changes induced by the secondary bile acid (DCA) through regulation of liver
PR:PROJECT_SUMMARY               	lipid metabolism.
PR:INSTITUTE                     	QIMR Berghofer Medical Research Institute
PR:DEPARTMENT                    	Precision & Systems Biomedicine
PR:LAST_NAME                     	Mohamed
PR:FIRST_NAME                    	Ahmed
PR:ADDRESS                       	300 Herston Road, Herston, QLD, 4006, Australia
PR:EMAIL                         	ahmed.mohamed@qimrberghofer.edu.au
PR:PHONE                         	+61738453992
#STUDY
ST:STUDY_TITLE                   	Effect of high-fat diet on serum lipidome in mice
ST:STUDY_SUMMARY                 	We analyzed mouse serum samples from a mouse dietary intervention experiment.
ST:STUDY_SUMMARY                 	Briefly, C57BL/6 mice (n=44) were divided into 4 groups (n=11 per group) and fed
ST:STUDY_SUMMARY                 	High-fat diet (HFD), 1% deoxycholic acid (DCA) in drinking water, both, or left
ST:STUDY_SUMMARY                 	as control for 9 months. For quality control, 12 TQC samples and 2 blanks were
ST:STUDY_SUMMARY                 	also included in the analysis (total 58 samples and 6 groups). The two
ST:STUDY_SUMMARY                 	treatments were selected to demonstrate the ability of lipidomics to detect
ST:STUDY_SUMMARY                 	gross changes induced by HFD in the serum lipidome, as well as specific/minor
ST:STUDY_SUMMARY                 	changes induced by the secondary bile acid (DCA) through regulation of liver
ST:STUDY_SUMMARY                 	lipid metabolism.
ST:INSTITUTE                     	QIMR Berghofer Medical Research Institute
ST:LAST_NAME                     	Mohamed
ST:FIRST_NAME                    	Ahmed
ST:ADDRESS                       	300 Herston Road
ST:EMAIL                         	ahmed.mohamed@qimrberghofer.edu.au
ST:PHONE                         	+61738453992
#SUBJECT
SU:SUBJECT_TYPE                  	Mammal
SU:SUBJECT_SPECIES               	Mus musculus
SU:TAXONOMY_ID                   	10090
SU:GENOTYPE_STRAIN               	C57BL/6
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data
SUBJECT_SAMPLE_FACTORS           	-	S1A	Diet:Normal | BileAcid:water	RAW_FILE_NAME=S1A.d
SUBJECT_SAMPLE_FACTORS           	-	S2A	Diet:Normal | BileAcid:water	RAW_FILE_NAME=S2A.d
SUBJECT_SAMPLE_FACTORS           	-	S3A	Diet:Normal | BileAcid:water	RAW_FILE_NAME=S3A.d
SUBJECT_SAMPLE_FACTORS           	-	S4A	Diet:Normal | BileAcid:water	RAW_FILE_NAME=S4A.d
SUBJECT_SAMPLE_FACTORS           	-	S5A	Diet:Normal | BileAcid:water	RAW_FILE_NAME=S5A.d
SUBJECT_SAMPLE_FACTORS           	-	S6A	Diet:Normal | BileAcid:water	RAW_FILE_NAME=S6A.d
SUBJECT_SAMPLE_FACTORS           	-	S7A	Diet:Normal | BileAcid:water	RAW_FILE_NAME=S7A.d
SUBJECT_SAMPLE_FACTORS           	-	S8A	Diet:Normal | BileAcid:water	RAW_FILE_NAME=S8A.d
SUBJECT_SAMPLE_FACTORS           	-	S9A	Diet:Normal | BileAcid:water	RAW_FILE_NAME=S9A.d
SUBJECT_SAMPLE_FACTORS           	-	S10A	Diet:Normal | BileAcid:water	RAW_FILE_NAME=S10A.d
SUBJECT_SAMPLE_FACTORS           	-	S11A	Diet:Normal | BileAcid:water	RAW_FILE_NAME=S11A.d
SUBJECT_SAMPLE_FACTORS           	-	S1B	Diet:Normal | BileAcid:DCA	RAW_FILE_NAME=S1B.d
SUBJECT_SAMPLE_FACTORS           	-	S2B	Diet:Normal | BileAcid:DCA	RAW_FILE_NAME=S2B.d
SUBJECT_SAMPLE_FACTORS           	-	S3B	Diet:Normal | BileAcid:DCA	RAW_FILE_NAME=S3B.d
SUBJECT_SAMPLE_FACTORS           	-	S4B	Diet:Normal | BileAcid:DCA	RAW_FILE_NAME=S4B.d
SUBJECT_SAMPLE_FACTORS           	-	S5B	Diet:Normal | BileAcid:DCA	RAW_FILE_NAME=S5B.d
SUBJECT_SAMPLE_FACTORS           	-	S6B	Diet:Normal | BileAcid:DCA	RAW_FILE_NAME=S6B.d
SUBJECT_SAMPLE_FACTORS           	-	S7B	Diet:Normal | BileAcid:DCA	RAW_FILE_NAME=S7B.d
SUBJECT_SAMPLE_FACTORS           	-	S8B	Diet:Normal | BileAcid:DCA	RAW_FILE_NAME=S8B.d
SUBJECT_SAMPLE_FACTORS           	-	S9B	Diet:Normal | BileAcid:DCA	RAW_FILE_NAME=S9B.d
SUBJECT_SAMPLE_FACTORS           	-	S10B	Diet:Normal | BileAcid:DCA	RAW_FILE_NAME=S10B.d
SUBJECT_SAMPLE_FACTORS           	-	S11B	Diet:Normal | BileAcid:DCA	RAW_FILE_NAME=S11B.d
SUBJECT_SAMPLE_FACTORS           	-	S1C	Diet:HighFat | BileAcid:water	RAW_FILE_NAME=S1C.d
SUBJECT_SAMPLE_FACTORS           	-	S2C	Diet:HighFat | BileAcid:water	RAW_FILE_NAME=S2C.d
SUBJECT_SAMPLE_FACTORS           	-	S3C	Diet:HighFat | BileAcid:water	RAW_FILE_NAME=S3C.d
SUBJECT_SAMPLE_FACTORS           	-	S4C	Diet:HighFat | BileAcid:water	RAW_FILE_NAME=S4C.d
SUBJECT_SAMPLE_FACTORS           	-	S5C	Diet:HighFat | BileAcid:water	RAW_FILE_NAME=S5C.d
SUBJECT_SAMPLE_FACTORS           	-	S6C	Diet:HighFat | BileAcid:water	RAW_FILE_NAME=S6C.d
SUBJECT_SAMPLE_FACTORS           	-	S7C	Diet:HighFat | BileAcid:water	RAW_FILE_NAME=S7C.d
SUBJECT_SAMPLE_FACTORS           	-	S8C	Diet:HighFat | BileAcid:water	RAW_FILE_NAME=S8C.d
SUBJECT_SAMPLE_FACTORS           	-	S9C	Diet:HighFat | BileAcid:water	RAW_FILE_NAME=S9C.d
SUBJECT_SAMPLE_FACTORS           	-	S10C	Diet:HighFat | BileAcid:water	RAW_FILE_NAME=S10C.d
SUBJECT_SAMPLE_FACTORS           	-	S11C	Diet:HighFat | BileAcid:water	RAW_FILE_NAME=S11C.d
SUBJECT_SAMPLE_FACTORS           	-	S1D	Diet:HighFat | BileAcid:DCA	RAW_FILE_NAME=S1D.d
SUBJECT_SAMPLE_FACTORS           	-	S2D	Diet:HighFat | BileAcid:DCA	RAW_FILE_NAME=S2D.d
SUBJECT_SAMPLE_FACTORS           	-	S3D	Diet:HighFat | BileAcid:DCA	RAW_FILE_NAME=S3D.d
SUBJECT_SAMPLE_FACTORS           	-	S4D	Diet:HighFat | BileAcid:DCA	RAW_FILE_NAME=S4D.d
SUBJECT_SAMPLE_FACTORS           	-	S5D	Diet:HighFat | BileAcid:DCA	RAW_FILE_NAME=S5D.d
SUBJECT_SAMPLE_FACTORS           	-	S6D	Diet:HighFat | BileAcid:DCA	RAW_FILE_NAME=S6D.d
SUBJECT_SAMPLE_FACTORS           	-	S7D	Diet:HighFat | BileAcid:DCA	RAW_FILE_NAME=S7D.d
SUBJECT_SAMPLE_FACTORS           	-	S8D	Diet:HighFat | BileAcid:DCA	RAW_FILE_NAME=S8D.d
SUBJECT_SAMPLE_FACTORS           	-	S9D	Diet:HighFat | BileAcid:DCA	RAW_FILE_NAME=S9D.d
SUBJECT_SAMPLE_FACTORS           	-	S10D	Diet:HighFat | BileAcid:DCA	RAW_FILE_NAME=S10D.d
SUBJECT_SAMPLE_FACTORS           	-	S11D	Diet:HighFat | BileAcid:DCA	RAW_FILE_NAME=S11D.d
SUBJECT_SAMPLE_FACTORS           	-	Blank_1	Diet:blank | BileAcid:blank	RAW_FILE_NAME=Blank_1.d
SUBJECT_SAMPLE_FACTORS           	-	Blank_2	Diet:blank | BileAcid:blank	RAW_FILE_NAME=Blank_2.d
SUBJECT_SAMPLE_FACTORS           	-	TQC_1	Diet:QC | BileAcid:QC	RAW_FILE_NAME=TQC_1.d
SUBJECT_SAMPLE_FACTORS           	-	TQC_2	Diet:QC | BileAcid:QC	RAW_FILE_NAME=TQC_2.d
SUBJECT_SAMPLE_FACTORS           	-	TQC_3	Diet:QC | BileAcid:QC	RAW_FILE_NAME=TQC_3.d
SUBJECT_SAMPLE_FACTORS           	-	TQC_4	Diet:QC | BileAcid:QC	RAW_FILE_NAME=TQC_4.d
SUBJECT_SAMPLE_FACTORS           	-	TQC_5	Diet:QC | BileAcid:QC	RAW_FILE_NAME=TQC_5.d
SUBJECT_SAMPLE_FACTORS           	-	TQC_6	Diet:QC | BileAcid:QC	RAW_FILE_NAME=TQC_6.d
SUBJECT_SAMPLE_FACTORS           	-	TQC_7	Diet:QC | BileAcid:QC	RAW_FILE_NAME=TQC_7.d
SUBJECT_SAMPLE_FACTORS           	-	TQC_8	Diet:QC | BileAcid:QC	RAW_FILE_NAME=TQC_8.d
SUBJECT_SAMPLE_FACTORS           	-	TQC_9	Diet:QC | BileAcid:QC	RAW_FILE_NAME=TQC_9.d
SUBJECT_SAMPLE_FACTORS           	-	TQC_10	Diet:QC | BileAcid:QC	RAW_FILE_NAME=TQC_10.d
SUBJECT_SAMPLE_FACTORS           	-	TQC_11	Diet:QC | BileAcid:QC	RAW_FILE_NAME=TQC_11.d
SUBJECT_SAMPLE_FACTORS           	-	TQC_12	Diet:QC | BileAcid:QC	RAW_FILE_NAME=TQC_12.d
#COLLECTION
CO:COLLECTION_SUMMARY            	Mouse serum lipids were extracted using the lipid extraction method by Matyash
CO:COLLECTION_SUMMARY            	et al(J Lipid Res 2008, 49, (5), 1137-46). Mouse serum (30 µL) was added to 215
CO:COLLECTION_SUMMARY            	µL of ice-cold methanol containing 50 µg/mL butylated hydroxytoluene (BHT).
CO:COLLECTION_SUMMARY            	Samples were homogenized by three rounds of vortex mixing for 30 seconds,
CO:COLLECTION_SUMMARY            	freezing in liquid nitrogen for one minute, thawing for two minutes and
CO:COLLECTION_SUMMARY            	sonicating for 10 minutes at 15°C, power 100% in a Grant XUB18 bath sonicator.
CO:SAMPLE_TYPE                   	Blood (serum)
CO:STORAGE_CONDITIONS            	-80℃
#TREATMENT
TR:TREATMENT_SUMMARY             	C57BL/6 mice (n=44) were divided into 4 groups (n=11 per group) and fed High-fat
TR:TREATMENT_SUMMARY             	diet (HFD), 1% deoxycholic acid (DCA) in drinking water, both, or left as
TR:TREATMENT_SUMMARY             	control for 9 months. Serum samples were collected and analyzed by targeted
TR:TREATMENT_SUMMARY             	lipidomics as described in Supplemental Information. The two treatments were
TR:TREATMENT_SUMMARY             	selected to demonstrate the ability of lipidomics to detect gross changes
TR:TREATMENT_SUMMARY             	induced by HFD in the serum lipidome, as well as specific/minor changes induced
TR:TREATMENT_SUMMARY             	by the secondary bile acid (DCA) through regulation of liver lipid metabolism.
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	Mouse serum lipids were extracted using the lipid extraction method by Matyash
SP:SAMPLEPREP_SUMMARY            	et al. Mouse serum (30 µL) was added to 215 µL of ice-cold methanol containing
SP:SAMPLEPREP_SUMMARY            	50 µg/mL butylated hydroxytoluene (BHT). Samples were homogenized by three
SP:SAMPLEPREP_SUMMARY            	rounds of vortex mixing for 30 seconds, freezing in liquid nitrogen for one
SP:SAMPLEPREP_SUMMARY            	minute, thawing for two minutes and sonicating for 10 minutes at 15°C, power
SP:SAMPLEPREP_SUMMARY            	100% in a Grant XUB18 bath sonicator. SPLASH LipidoMix Mass Spec Standard (10
SP:SAMPLEPREP_SUMMARY            	µL, undiluted) and Cer/Sph mixture II (10 µL, undiluted) internal standards
SP:SAMPLEPREP_SUMMARY            	mixes from Avanti Polar Lipids were then added to each sample. After overnight
SP:SAMPLEPREP_SUMMARY            	incubation at -30°C, 750 µL MTBE was added and each tube was vortex mixed for
SP:SAMPLEPREP_SUMMARY            	10 seconds and shaken for 10 minutes on a tube rotator (4°C). MilliQ water (188
SP:SAMPLEPREP_SUMMARY            	µL) was then added, and the tube was vortex mixed for 30 seconds to form a
SP:SAMPLEPREP_SUMMARY            	biphasic separation. After centrifuging for 15 minutes at 15,000 ×g, the clear
SP:SAMPLEPREP_SUMMARY            	upper phase containing lipids in MTBE was transferred to another tube and dried
SP:SAMPLEPREP_SUMMARY            	down using a gentle stream of nitrogen. Prior to LC-MS/MS analysis, the samples
SP:SAMPLEPREP_SUMMARY            	were resuspended in a mixture of 50 µL methanol (containing 50 µg/mL
SP:SAMPLEPREP_SUMMARY            	BHT)/toluene (90/10%, v/v) and 4µL was used as the injection volume.
SP:PROCESSING_STORAGE_CONDITIONS 	On ice
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_SUMMARY        	buffer A contained 50% acetonitrile and buffer B contained 95% acetonitrile in
CH:CHROMATOGRAPHY_SUMMARY        	water. Buffers were supplemented with 25 mM ammonium formate (pH 4.6) and 0.1%
CH:CHROMATOGRAPHY_SUMMARY        	formic acid.
CH:CHROMATOGRAPHY_TYPE           	HILIC
CH:INSTRUMENT_NAME               	Agilent 1290 Infinity
CH:COLUMN_NAME                   	Agilent HILIC Plus RRHD (100 x 2.1mm, 1.8um)
#ANALYSIS
AN:ANALYSIS_TYPE                 	MS
#MS
MS:INSTRUMENT_NAME               	Agilent 6490 QQQ
MS:INSTRUMENT_TYPE               	Triple quadrupole
MS:MS_TYPE                       	ESI
MS:ION_MODE                      	POSITIVE
MS:MS_COMMENTS                   	Targeted lipidomics were performed on an Agilent Technologies 1290 Infinity
MS:MS_COMMENTS                   	UHPLC system with an Agilent HILIC Plus RRHD 2.1×100 mm 1.8-micron column,
MS:MS_COMMENTS                   	coupled online to an Agilent 6490A Triple Quadrupole mass spectrometer with
MS:MS_COMMENTS                   	iFunnel and Agilent Jet Stream (AJS) electrospray ionization (ESI) source,
MS:MS_COMMENTS                   	operated in dynamic MRM mode. The source nitrogen gas temperature was set to
MS:MS_COMMENTS                   	250°C at a flow of 15 L/min and a sheath gas temperature of 400°C at a flow of
MS:MS_COMMENTS                   	12 L/min. The capillary voltage was set to 4000 V for positive mode and 5000 V
MS:MS_COMMENTS                   	for negative mode and the nebulizer operated at 30 psi. Ion funnel high and low
MS:MS_COMMENTS                   	pressure in positive and negative mode were 150 and 120. Check tunes were
MS:MS_COMMENTS                   	performed in wide, unit and enhanced modes prior to each experiment to confirm
MS:MS_COMMENTS                   	the performance of the mass spectrometer. The quadrupole was tuned to reference
MS:MS_COMMENTS                   	masses 118.09, 322.05, 622.03, 922.01 and 1221.99 in positive ionization mode;
MS:MS_COMMENTS                   	112.99, 302.00, 601.98, 1033.99 and 1333.97 in negative ionization mode.
#MS_METABOLITE_DATA
MS_METABOLITE_DATA:UNITS	intensity
MS_METABOLITE_DATA_START
Samples	S1A	S2A	S3A	S4A	S5A	S6A	S7A	S8A	S9A	S10A	S11A	S1B	S2B	S3B	S4B	S5B	S6B	S7B	S8B	S9B	S10B	S11B	S1C	S2C	S3C	S4C	S5C	S6C	S7C	S8C	S9C	S10C	S11C	S1D	S2D	S3D	S4D	S5D	S6D	S7D	S8D	S9D	S10D	S11D	Blank_1	Blank_2	TQC_1	TQC_2	TQC_3	TQC_4	TQC_5	TQC_6	TQC_7	TQC_8	TQC_9	TQC_10	TQC_11	TQC_12
Factors	Diet:Normal | BileAcid:water	Diet:Normal | BileAcid:water	Diet:Normal | BileAcid:water	Diet:Normal | BileAcid:water	Diet:Normal | BileAcid:water	Diet:Normal | BileAcid:water	Diet:Normal | BileAcid:water	Diet:Normal | BileAcid:water	Diet:Normal | BileAcid:water	Diet:Normal | BileAcid:water	Diet:Normal | BileAcid:water	Diet:Normal | BileAcid:DCA	Diet:Normal | BileAcid:DCA	Diet:Normal | BileAcid:DCA	Diet:Normal | BileAcid:DCA	Diet:Normal | BileAcid:DCA	Diet:Normal | BileAcid:DCA	Diet:Normal | BileAcid:DCA	Diet:Normal | BileAcid:DCA	Diet:Normal | BileAcid:DCA	Diet:Normal | BileAcid:DCA	Diet:Normal | BileAcid:DCA	Diet:HighFat | BileAcid:water	Diet:HighFat | BileAcid:water	Diet:HighFat | BileAcid:water	Diet:HighFat | BileAcid:water	Diet:HighFat | BileAcid:water	Diet:HighFat | BileAcid:water	Diet:HighFat | BileAcid:water	Diet:HighFat | BileAcid:water	Diet:HighFat | BileAcid:water	Diet:HighFat | BileAcid:water	Diet:HighFat | BileAcid:water	Diet:HighFat | BileAcid:DCA	Diet:HighFat | BileAcid:DCA	Diet:HighFat | BileAcid:DCA	Diet:HighFat | BileAcid:DCA	Diet:HighFat | BileAcid:DCA	Diet:HighFat | BileAcid:DCA	Diet:HighFat | BileAcid:DCA	Diet:HighFat | BileAcid:DCA	Diet:HighFat | BileAcid:DCA	Diet:HighFat | BileAcid:DCA	Diet:HighFat | BileAcid:DCA	Diet:blank | BileAcid:blank	Diet:blank | BileAcid:blank	Diet:QC | BileAcid:QC	Diet:QC | BileAcid:QC	Diet:QC | BileAcid:QC	Diet:QC | BileAcid:QC	Diet:QC | BileAcid:QC	Diet:QC | BileAcid:QC	Diet:QC | BileAcid:QC	Diet:QC | BileAcid:QC	Diet:QC | BileAcid:QC	Diet:QC | BileAcid:QC	Diet:QC | BileAcid:QC	Diet:QC | BileAcid:QC
LPC 18:1(d7)	1603849	1581032	1600005	1695387	1465645	1636445	1376774	1767487	1539081	1840203	1670646	1408949	1634418	1879388	1974894	1535695	1888508	1819749	1746384	1763604	1660647	1595085	1019896	1233172	1251974	1259922	1078267	993273	1423639	1228218	1204068	1163541	877594	1522280	1747300	1151871	1313185	1316070	2447729	1440231	1534797	1604056	1668310	1689275	163	113	1359	1592	1615	1895	691	1768	2019	1958	2426	2414	2238	2371
LPC 14:0	22126	28497	27138	18417	24130	29301	26282	21445	26397	22157	25207	73009	40757	41657	38364	41035	62841	46699	38409	37622	37360	36723	15623	16324	15043	15092	20225	16217	21576	15613	22508	23623	17490	25150	24086	20395	18980	18180	21022	20622	20168	24504	21555	19786	262	128	21887	21432	20645	20984	20967	20006	22189	23111	22680	23619	22449	23608
LPC 16:0	81086552	81251024	63627568	77089160	80788904	85888928	75634056	73151064	76055928	63727624	73839032	76181016	80974648	74980448	66583168	82754480	74340248	75017320	77119352	74661224	80104072	75465480	80250376	77755584	73363936	77060376	85288296	87986592	66388816	77433880	86382096	81864968	83046616	70296976	59969860	75736400	70606440	77381608	71738840	73265544	74162800	71920152	69800872	57861448	60089	63137	68195976	67685872	67956760	67870888	68726328	70267080	73735272	74420736	75366520	76737072	77179888	79096744
LPC 16:1	3071952	3572977	2752344	2376122	3464364	2684225	3445417	3398876	3153821	2649190	3206263	5038443	4533861	4267147	4925561	5563660	4295792	5361596	5519924	5988015	5738762	5577604	2190621	1891395	2129439	1821580	2853612	2514998	2284806	2075347	2494664	2190315	3260623	2336628	2120035	2890456	2316856	2890687	2542919	2905160	2634707	2732754	2274145	1775611	1369	2540	2871854	2894272	2815516	2896038	2960510	2944976	3087477	3064457	3158173	3225785	3202608	3284994
LPC 18:0	40955684	38107916	25790836	37962420	35516948	39465312	32870852	32919356	32731942	28183446	28706382	20339188	27276518	19347082	19903626	21711940	20422852	18799754	26423508	23950742	28034880	25636778	56890764	50405068	50817688	51782868	52917260	54973748	35563532	52866560	52587588	55040324	44279756	37672876	35408872	42310456	37846980	38810356	37854672	41251796	44840540	40773800	38011880	32623740	32349	30711	35438064	34507264	34515264	34586276	35774544	34915796	37440904	37446600	40731908	39065488	40332732	40725828
LPC 18:1	41000184	40302324	38572612	39807812	40210348	38245152	41316216	40800372	38652996	41845516	38402052	51982128	42732568	47205452	44894556	53403780	45422832	45242432	44175456	48985332	46863116	46305008	38074296	34495564	39150448	32783154	46318340	46870404	40661136	36084580	42105288	37789716	54758668	37805376	39941152	50300924	44496316	53618308	46225776	48796696	43703288	43754392	44405128	39352228	27147	32829	40636892	40984572	39868284	39353488	40462604	40486592	43231320	43839920	45793908	45173320	46593032	48055136
LPC 18:2	44892640	42097032	40552100	42635536	41699096	45054192	41446504	41421732	41189852	50569800	36845268	45793080	40192500	34773240	39903584	41048060	34917640	32386002	31750898	36914876	34428652	33638692	43750760	37971920	49381596	35715040	38372476	42502000	42900564	49572360	43327152	42126064	45720028	42187024	43014572	44508156	41851576	33795004	45167292	43935484	35100628	36890120	35630588	34426996	21665	25979	36267920	35586848	35066896	36542560	35686904	35669724	39923052	39618152	40036156	41760892	41651768	43131976
LPC 20:0	1254552	1448722	910493	1897974	1574802	2007047	1278581	1215500	1520365	1479071	1459092	901662	856038	627150	913053	775340	784733	679969	1325566	1118022	1298121	1178267	739988	688351	679819	843861	507381	346346	411511	784847	591977	660198	460829	821829	1128578	383147	491907	284887	562258	783883	802109	730708	603801	661654	1811	1771	685971	681818	664808	708507	720813	713115	746877	788032	792233	804569	794340	810599
LPC 20:1	1760017	1848933	1460968	1641930	1682634	1685180	1766254	1720312	1694272	1492591	1654441	1444615	1494072	1132422	1601336	1437151	1529789	1390480	1927090	1874076	1909163	1928553	1038811	986308	1003613	1045241	1066500	924710	906694	1054548	1106913	1107715	1167967	1164261	1205055	945565	987142	913105	964356	1329135	1305994	1284576	1236336	999520	1025	2858	1152329	1140449	1192278	1130215	1168845	1230590	1306734	1313839	1289484	1332998	1326581	1360128
LPC 20:2	1472863	1284226	1138538	977622	1250095	902566	1340712	1271377	1218604	1109649	1078948	781811	923047	778889	945488	939411	769477	869679	952059	1003760	900873	953727	1349771	1110804	1495094	1121902	1340127	1374441	1210134	1360148	1260062	1182086	1712087	1062270	1083345	1336673	1196718	1267911	1124383	1466818	1209212	1176243	1001698	913999	180	433	1113352	1117800	1128711	1075637	1128541	1089489	1208407	1261591	1265639	1229490	1306241	1270600
LPC 20:3	11362663	8793557	8632598	5982569	7888215	5554479	9187480	8843999	8129615	7883263	7094471	5774545	7084531	6022789	6959119	6965162	5889557	6629057	6152899	6817664	5651559	6567714	12312874	9289400	14310234	9921169	12106161	13335363	11100179	12410816	10782096	9604951	15166592	8388595	8368372	11616150	10530391	12144768	9296660	11334936	10579893	10171531	8782898	7198627	3906	3583	9345728	9140932	9191148	9340314	9362309	9302146	9806544	9774077	10298754	10410019	10374224	10768024
LPC 20:4	17482664	13383533	16008231	8100404	11159028	9451223	12336227	15382918	11296997	14804728	10728347	9723011	9723321	8160663	8308957	6249612	5705477	8223146	6315235	6702610	6229675	6582292	33035992	31369834	35866184	28715796	35544652	41789852	34769412	31963060	35296756	31728832	34010836	20797736	16288739	30123608	27726924	30864816	25552870	24087806	24943552	22015340	21444460	16443780	9582	7171	20326436	20816192	20728822	20306874	21424086	20797128	22434532	21840582	23261232	22942812	24672728	24474676
LPC 20:5	1387767	1485649	857184	1094414	1430790	1257209	1289769	1237368	1206837	1192302	1148721	871478	1259397	825611	1535467	989546	593500	802567	1098699	1233804	1207295	1287030	701153	566902	855370	563279	753386	827460	613567	776199	715753	638996	1065861	840135	954870	1011649	862742	931648	883969	811582	830445	913583	743421	788335	458	287	874941	878205	893261	897077	931987	903848	949883	972431	969869	1043015	1008620	1091648
LPC 22:0	414150	619206	523193	464468	459667	565013	479299	543545	531840	512041	631922	330038	363843	286981	530287	271884	456735	364199	412094	406747	316516	506998	1276962	1202275	1306771	1178254	949068	1135847	702715	857439	1052436	1824917	1285168	635929	712513	734485	768145	663685	481632	465724	697150	733862	1033300	1153753	9174	18308	397432	404800	425523	457914	562064	640087	672597	605935	662224	723802	774380	777286
LPC 22:1	145134	167568	143906	174020	173396	194529	161612	164707	174770	176867	176691	129236	125839	105853	149706	106923	115853	102017	204193	180349	195539	184868	97385	104271	101057	113578	77129	76961	82205	106413	93350	108951	84705	106639	139477	64843	76924	65319	82850	100805	104445	96740	95621	97675	95	435	100804	95278	97226	97421	106364	105489	107248	111459	118901	113474	119156	118965
LPC 22:2	23390	24471	24088	22278	25452	22470	18277	22362	26251	30070	23688	20069	15484	16380	19086	19297	19862	19153	22882	20889	22011	20929	17882	15570	18828	20287	10951	14639	13241	15367	13170	17497	13889	16329	16586	13945	11641	12167	11768	15407	15576	15923	12800	14194	42	90	13452	13115	12236	16904	13624	14246	18563	17550	14961	18234	16550	19387
LPC 22:3	40730	38056	42282	31613	38711	30222	41382	38465	34872	36347	35045	29535	26128	30172	26937	26744	24914	28585	31924	29587	28873	28417	49955	41429	50653	44104	48313	52194	49948	43455	41097	36999	53232	31161	28678	45959	41613	44750	36706	47168	39330	39141	33255	30024	34	49	37284	38501	36764	36744	36768	36971	40101	40095	41446	39805	42482	42125
LPC 22:4	328983	318546	282753	224499	318292	186286	334602	278374	275393	251299	254824	221071	166636	218084	181198	193230	151103	177172	194386	169732	182042	178182	466305	392197	536196	402459	481319	511389	404180	411888	391455	352247	514905	229409	238269	401562	372450	405020	335855	384170	325792	322445	265961	259515	149	98	303010	310906	303950	307880	311161	308345	329753	345192	345212	364598	363904	357650
LPC 22:5	2893400	2736792	2113405	2043342	2798188	1730398	2899024	2517611	2329408	2324587	2335969	2373655	1689699	2119834	1876194	1841299	1345527	1494579	1696258	1694364	1676088	1674856	2627861	2438756	2993763	2515823	2667025	3048641	1937208	2805365	2421026	2192108	2796145	1561594	1756170	2573813	2431769	2308221	2109952	2077918	2327668	1931346	1684659	1785993	364	319	2075696	2090272	2177173	2172984	2139958	2166844	2270464	2324913	2327455	2335627	2412990	2388036
LPC 22:6	21636128	17699582	14023841	12553743	15257860	11444690	17219260	17551066	13376466	16784354	12959568	12229519	11608781	11914300	12369093	9096606	8566223	9032050	9172927	9603417	9355497	9749237	17691382	17582776	20187398	16719722	16128351	21321972	11934185	21861054	17820018	16474065	19641214	12366489	11432863	18046182	16654844	16850432	14569770	13876706	17146606	13663442	12095783	12141811	2905	2731	14139866	14021866	13605528	13258240	14446397	14702745	14742821	15404511	15880728	15721619	16585971	16804442
LPC 24:0	478214	571501	436133	521462	468764	534696	501489	499644	496144	584223	585045	318461	343762	265727	390597	299017	284698	284271	369985	368776	356479	372403	895137	825850	936663	775158	643671	784510	528256	699103	759375	1104277	738153	431399	520995	445835	455973	406075	375464	382910	547123	486772	529911	595189	5544	6745	388134	400528	394638	439943	481921	516576	534766	537349	573528	558880	604993	621747
LPC 24:1	116994	142904	118206	141536	130237	147648	136590	134128	131594	141623	143905	112906	106456	92443	135467	91449	120013	98248	143123	128313	136674	137525	139312	143718	155077	140777	121058	139451	111967	127468	135436	196516	149486	99563	125392	94566	100841	95553	82166	90228	110926	111297	135706	150177	1187	2243	88847	91441	92099	91282	103298	116464	117377	117153	122594	124602	135165	129342
LPC 26:0	42539	54256	64994	38636	33440	39981	43260	53043	44433	46144	67546	38647	35787	42439	48797	42524	44232	52169	37040	61166	31461	51419	116670	127566	137881	98443	91037	93641	80814	88066	110699	141768	119509	81189	79144	79864	81494	84622	75598	65125	74418	76491	95561	100131	504	3653	29875	33880	37065	49925	68049	84562	80446	75114	77642	92208	93822	102580
LPE 18:1(d7)	52585	41325	41386	46552	40611	42524	39860	42948	37628	38211	44273	36324	41052	40392	42816	41169	38042	44279	36556	42420	38810	36817	38091	40194	41769	41629	42061	34659	39907	39180	41987	37175	35184	38802	43908	40115	38995	43813	71048	42963	42928	49038	43922	36212	NA	35	29	40	10	18	54	40	67	63	52	89	25	99
LPE 16:0	446002	374697	294191	459100	448765	402277	459246	340593	377254	373664	408275	296352	375412	237052	325101	359653	308971	330404	345519	385044	414205	314967	288021	237906	240605	252436	230579	337554	256327	312410	272547	282777	353716	207906	232032	306593	248504	300696	246013	296213	319295	321310	278775	267693	132	68	314728	329537	311716	295841	359168	321844	344961	370296	324296	361045	328180	314815
LPE 16:1	5589	7975	5496	7470	7679	7282	7612	6250	8482	8374	7792	6476	9014	9538	10304	11074	8452	10083	13759	13571	17686	12757	4166	4141	4581	3285	3419	4910	4140	5102	3735	4229	6610	3455	4166	7378	5163	5054	5315	5472	3769	4506	4424	3746	1	0	7127	5479	5755	5066	5618	6595	6527	6456	7340	7290	6823	6504
LPE 18:0	91135	73213	62477	92526	96940	87668	86479	76748	83600	75102	83502	56694	65175	48570	47721	60150	45171	47572	61426	78133	68977	63477	116941	87994	118158	101271	100874	134029	73747	137061	100686	106225	123078	70409	71475	96867	81084	92920	76551	89987	77032	92502	79347	65216	62	71	81892	80225	76256	80583	78285	76972	82325	80480	100543	89293	86006	92093
LPE 18:1	121835	112389	133135	130231	143316	167506	132750	80198	125054	125624	102431	179877	135911	161267	116089	194968	129330	109322	162609	178983	210349	132412	76636	102708	87351	70599	65797	102212	74721	122302	71619	88537	95822	69734	72399	106096	90200	77084	78397	78272	55588	71640	72537	64737	70	194	98846	94163	94755	104536	94822	96099	104645	117284	106948	112153	101011	114032
LPE 18:2	264970	221887	238853	335938	287971	341499	273125	193406	264038	299382	287319	265221	232944	210016	184376	294673	235750	138095	195568	205872	247045	172436	240250	245436	275615	165409	134564	257227	169127	330360	181169	244992	282734	202561	211638	448298	371149	167555	296062	265173	110751	173337	185973	149729	28	10	234195	226563	220732	203660	215651	217888	239597	239906	240425	249533	231750	230925
LPE 20:0	3088	3441	2522	4621	2896	3859	3627	3169	3258	4137	3657	2692	1968	1285	1994	2014	1813	1325	2521	3011	2974	2159	1440	1506	1532	1645	1255	857	1050	1615	1391	1777	1363	1546	1782	1173	1057	855	1476	1411	1470	1628	1202	1199	30	41	1284	1596	1712	2134	1698	1259	1259	1964	1632	1588	2059	1756
LPE 20:1	7044	7321	4753	6575	8457	6444	7876	6688	6526	6959	6662	2930	4542	2778	4176	2834	3198	2715	4364	5052	5276	4906	3958	3986	4663	4609	3050	4633	3133	5112	3866	4118	5163	2895	3511	4202	3770	3309	3739	4442	3685	4216	2725	3077	0	12	4857	4054	4499	5020	3791	4594	4222	4348	5174	5384	5523	5810
LPE 20:2	3153	3994	2411	3334	4469	2680	4093	3782	3088	4890	3494	1401	2118	2409	2988	2466	2076	1728	2553	2749	2811	2118	2269	2396	2606	2064	1723	3148	2058	3260	1670	2390	3682	1867	2672	3926	3294	2396	2263	2677	2478	1983	1611	1120	17	0	2548	2512	2394	2564	2838	2895	2643	2673	2334	2978	2631	2974
LPE 20:3	37483	40697	20430	35661	43945	25858	49064	32595	36639	40481	33536	16460	23483	23982	26189	26969	23706	21443	27272	25176	28784	21914	29777	29898	31452	29629	19594	39653	22479	41965	23070	28178	35684	25581	26008	41358	31419	28936	28683	30263	27049	25555	27080	20901	1	0	30774	28226	32750	32512	30899	28530	30652	30160	31595	31879	31839	28972
LPE 20:4	271203	251288	153238	220673	276511	191372	290533	248376	228648	263371	214770	77860	107755	105268	99375	86080	86471	82170	95491	106274	123359	92600	296291	313426	352732	299836	221564	412521	240457	391091	255843	302860	366377	203954	183596	353843	277353	290257	263596	262342	233993	227041	254807	184244	51	41	259249	234989	243344	233396	273502	275690	274695	254506	285059	274734	262141	283933
LPE 20:5	17514	22061	6511	18431	26728	16947	22564	16954	17890	18834	19905	5947	10029	7931	11783	10098	7422	5582	10795	12361	13065	10488	6542	5271	8495	6802	4394	8103	4459	11154	5419	7238	10455	7828	8562	12644	8629	8440	8777	7646	7781	7846	6339	7351	4	1	11575	10086	10228	10552	11186	9407	10804	11330	11496	11793	10883	11634
LPE 22:4	3599	3963	1760	3889	5271	2268	4547	3177	4272	4537	3392	1983	1628	2736	1728	2431	1387	1941	2501	2310	2754	2152	3924	3835	3632	3415	2039	3580	3119	3275	2550	3439	4096	2132	2752	4666	3550	3833	2952	3527	2549	2381	2620	2305	0	1	2710	2547	2550	1883	3262	3293	2485	2843	3581	3504	3665	3034
LPE 22:5	44246	46829	20021	49213	58599	29714	52703	38484	38851	44430	44720	20996	22720	32279	23023	27844	18796	17542	29012	25115	35742	23561	25740	25380	30185	31880	18258	32819	19266	35524	20424	20575	33163	19003	26235	34190	31427	24744	27219	24560	30650	23213	22348	24724	0	0	29873	31349	30990	29864	30600	28570	32041	29079	31209	35084	34493	34053
LPE 22:6	513698	484681	241294	431088	459015	313803	517001	489061	388745	505353	418222	172178	247022	263236	228896	233505	187005	195752	228215	228770	276055	241881	288444	316834	375876	396604	225487	375237	209049	389236	278532	310583	382013	243647	272587	433489	382006	388576	326682	284737	471478	288630	291849	295113	3	126	358893	366612	351929	348391	342431	375780	352060	371842	384849	371246	380489	357502
PC(O-32:0)	610748	745249	716785	779117	762418	765347	695984	798276	782352	910352	682526	1171930	748641	919951	784006	794095	1020499	911018	735843	720975	650690	728374	731776	730473	610757	726677	633451	654233	813923	679951	631290	598676	645070	609883	669585	599157	720112	614679	691284	599280	701338	605957	603010	614100	177	37	661201	582332	619452	635075	647364	652443	673229	701232	656573	689301	706157	721004
PC(O-34:1)	579188	650163	565010	705076	798405	726802	702264	703804	705635	860836	672012	2079759	902157	1527409	1063797	1335080	1784564	1311975	916156	825752	885333	964196	566635	562064	600283	592862	620998	687590	640006	580735	608316	603090	666118	657790	777302	666729	652944	659307	732898	685184	718483	672486	587147	671635	3910	3255	639438	678843	688455	631466	771041	699660	731220	768085	738480	802304	819565	788269
PC(O-34:2)	361772	490953	443158	514525	508165	522849	418006	503314	480698	700547	437881	2832468	674000	1172668	759965	1072673	2214561	999520	489657	550720	512150	557230	253013	261567	260049	259178	184632	227401	235542	277863	217994	260244	211411	372596	472170	231900	255236	202152	267829	256143	296802	264073	280308	358304	1395	1714	354478	351498	343862	355705	369497	380611	375964	378188	366889	379685	385496	444968
PC(O-34:3)	21902	34898	24943	48244	41441	34016	26948	22868	35143	30998	29158	32032	26446	16989	17108	36253	24104	19317	29221	28014	31864	35395	8260	6650	7274	5189	4211	3748	8271	6338	6392	3643	4454	8602	10221	7226	6760	6501	6330	9360	12603	8583	5235	6790	101	57	10817	10619	11126	10443	12130	10063	10457	12088	12530	12050	13425	13244
PC(O-36:2)	678462	839325	709639	773712	794506	787463	619505	777909	651906	961531	653904	1845564	766011	1039951	828176	1081266	1583537	967780	650689	673647	587276	665342	625547	589661	673810	597774	534031	560620	523541	733900	550044	656695	561587	640350	731496	569416	547387	446589	607815	579952	635661	590388	561719	648098	7321	7949	581362	550145	599104	603597	650940	639193	693918	676638	700430	708199	691834	723153
PC(O-36:3)	100678	119794	105019	144191	118328	132635	116061	124352	118845	174204	120694	314821	132665	209779	190408	203497	307364	192983	156903	158457	126919	156554	69984	66135	65916	64966	67223	61436	85080	88060	69938	70971	67523	89245	126801	77910	88782	69752	99026	93351	90732	88651	89127	97533	706	764	85077	96236	90832	93780	91060	90854	87172	87488	106751	104118	93938	108999
PC(O-36:4)	208162	205575	215164	263982	232217	288695	195337	200164	201785	234498	184243	407111	223685	254590	182731	255629	286638	238939	209036	189514	210846	211286	142615	139689	144285	150619	127639	133136	204091	151552	147104	113427	135957	207741	188427	156374	141405	148979	193090	194263	181125	169821	166887	153565	661	481	154441	154296	166934	138267	152734	165846	184746	172496	168511	189547	175972	176483
PC(O-36:5)	294494	338114	273151	348401	377858	316971	301932	287862	377948	369301	287556	262027	242854	218699	196289	253488	158726	172914	262182	219840	270196	246555	369374	317409	341905	335043	260747	388424	393889	320007	263397	274068	305114	285815	324976	438471	385308	509116	369733	460983	501821	358310	311412	272876	59	192	316165	339422	321095	338304	323306	392704	339856	360762	358723	367549	386579	370622
PC(O-38:0)	556335	739612	433263	519011	672893	517090	658003	599834	616558	662279	684473	838735	622515	719483	747709	745204	603577	759618	878631	828791	761059	795969	349383	382331	420872	371931	386528	394397	327428	448102	378502	347511	409549	429227	525574	426906	464683	452002	519757	467462	482525	451224	520609	446946	1880	1623	410844	411291	425066	473060	418638	468761	491369	477921	445768	452293	469929	475840
PC(O-38:2)	255435	322556	338904	442832	266220	374322	265423	243179	212533	326594	269511	300011	219993	292142	280283	285099	303735	282790	269551	211126	210088	226773	70840	76622	85029	78256	79029	69929	87853	111645	82098	92488	76878	93210	134662	89823	89319	61307	114561	111916	102049	100540	86101	91917	180	NA	128194	133473	137939	134780	146104	143271	146049	154166	154918	168291	146344	149153
PC(O-38:3)	86971	112711	110394	99156	102479	96602	98158	112524	108957	112586	98041	224259	116073	171609	124812	166125	244380	175398	102847	107055	116555	109737	88555	80492	80401	80968	71977	66562	78724	96793	78184	80912	75640	101313	120061	83578	78359	72997	90900	95681	91189	88980	82657	95305	11	246	74772	84826	89725	91449	79248	85361	90490	82973	84693	97769	87204	95351
PC(O-38:4)	203457	219788	194540	199187	207702	233713	180413	226782	176147	264856	168732	545834	222570	294657	205995	320016	367821	274217	196657	168535	172670	189832	213121	213468	222972	224264	188079	191225	246287	262092	218541	207651	191998	246584	290098	183579	176011	182137	254464	203469	201486	232670	240765	195542	1548	1520	193141	209397	173505	194773	179718	212515	195772	219593	201768	226818	216836	233690
PC(O-38:5)	384178	377370	361266	421472	490691	529660	386414	385341	421032	451868	385926	427430	334929	354940	342947	433437	305186	295823	345127	296911	344040	309019	442296	472053	393812	448882	345522	456489	549766	451100	371478	365017	354344	425869	419622	369996	420451	325399	393405	429281	319156	347819	415826	323338	1146	807	355053	335026	314755	349235	345345	368272	392352	384540	371341	427426	427008	397317
PC(O-40:0)	1000430	1062022	765564	651084	999470	698131	1011195	950859	751793	1001239	912486	938081	908788	1226709	1062966	921298	746944	888152	1032565	932498	889279	939897	696716	895194	833760	868291	873561	943174	753114	1084717	862729	1054377	1045715	895242	796176	746212	752148	865603	963871	811336	1038034	961247	1010723	821286	1652	1084	741806	830505	847892	891512	928509	788728	917200	707968	942345	963254	963096	965882
PC(O-40:1)	591640	614754	520465	559198	579140	440767	493715	643305	513181	624505	543404	457501	505293	589068	540652	555975	374369	437110	564048	518843	529100	488101	1524266	1369935	1604701	1321612	1356571	2243613	1250682	1501498	1259615	1156057	1429505	856985	1055808	1151719	884913	1079291	980793	970825	1169960	811962	1052358	828608	5	607	901795	820798	1064265	970873	759702	913766	1082493	864847	1002681	1075704	1124183	1004749
PC(O-40:2)	64817	75751	45903	64410	58426	58305	57986	62970	65574	84670	71084	60886	48403	53748	65896	52808	46131	50167	57693	56291	59076	61913	65796	53394	82225	64992	57661	84575	36102	73380	50694	54022	76214	56411	73353	66846	52114	69049	63192	52691	82905	63548	51959	54471	40	68	60027	50782	51519	54310	58912	57842	59313	57388	61124	53418	60442	59639
PC(O-40:3)	37326	42155	37156	43855	41147	42782	37135	41656	36614	44939	34644	40190	43206	39282	38875	40886	43922	41556	42159	35643	37389	38485	22886	22462	23919	21666	17244	16256	21525	23880	23496	22624	18894	24006	30630	19707	17325	15589	24018	24322	21663	20744	23008	20337	43	17	23708	19259	22464	20720	23530	21609	26336	24779	26707	26857	30257	26372
PC(O-40:4)	88623	102760	124428	119014	107074	112538	93565	110123	114605	152137	89024	110756	76154	95984	88448	88957	77097	87352	93637	74686	79094	77088	88469	108192	90098	94154	74432	73843	118931	106244	101924	88513	82581	100476	115724	76500	86224	69703	105978	96676	94837	113008	90614	86264	654	848	80015	71298	77616	74973	83701	82756	88443	91081	90293	87245	97279	104209
PC(O-40:5)	91515	100703	96076	105653	115548	105505	96394	105466	115232	116681	83413	200062	92634	128354	97519	137242	148546	128064	87616	87147	96285	88290	102856	112132	96171	104733	92478	115104	111601	99398	92401	100102	84822	105217	125257	90126	87106	74111	93045	100069	97478	83648	104700	91285	646	425	86997	86289	80874	91135	80274	79061	95235	101605	95280	101404	103021	96832
PC(O-40:6)	274241	304911	235911	340636	337533	345393	345071	289344	287276	338877	266557	738198	332892	514389	328329	516911	706578	411704	274211	291343	308804	288934	244059	252386	241064	259847	176253	219516	215455	286031	207297	206554	224073	278683	372043	217482	261984	186085	289209	245543	268344	227650	289172	283155	351	1121	243946	223051	242249	230500	236064	212710	243542	260530	275382	261736	297906	279956
PC(O-40:7)	498422	473894	425238	614191	540125	443501	446668	523712	518061	542763	449171	432788	361250	403691	405378	458910	291856	319392	419430	359937	421775	371592	442821	488307	422020	506812	310992	437057	387839	484799	328805	391401	411365	370446	504942	375622	419920	343756	417779	444788	479180	335687	432768	399905	237	946	367598	336690	413255	367134	406701	344860	433154	428612	431857	461404	520770	470118
PC(P-32:0)	40154	40299	41789	35463	48177	37677	45521	40460	45223	70265	43059	81380	53805	86590	79095	82603	88563	69430	61292	55066	55646	61464	29053	30265	30459	30621	28701	27926	41058	30145	31194	28723	28799	33302	45464	28657	30413	30460	34745	35647	38980	38231	27989	30115	79	135	36979	43706	40413	37119	42744	45804	42420	41239	44009	44630	44423	47511
PC(P-34:1)	363385	437704	378509	484243	466643	465862	376458	418242	425641	622636	404801	2610838	616550	1087923	655229	970572	2086630	874752	459044	504662	465049	522139	199339	218027	211096	186122	134295	175128	203577	234479	160314	203041	158071	328302	428156	186136	217639	148084	207066	219662	230751	198446	230330	301536	890	1152	298115	323752	289395	299025	304378	343255	334699	325929	276258	342693	320086	382731
PC(P-34:2)	21902	34898	24943	48244	41441	34016	26948	22868	35143	30998	29158	32032	26446	16989	17108	36253	24104	19317	29221	28014	31864	35395	8159	6706	7274	5189	4594	3770	8271	6330	6377	3369	4527	8602	10221	7191	6760	6547	6330	9360	12603	8583	5147	6790	101	57	10817	10619	11126	10443	12130	10063	10457	12088	12530	12050	13425	13244
PC(P-34:3)	6123	5595	6588	10033	5886	8617	5806	4891	6488	6168	6564	9010	7126	5173	5409	7873	6110	5024	6109	5579	7981	8944	2843	1709	1992	1455	1498	1244	1559	1503	1673	1668	1617	2208	2152	2336	1850	1846	2085	2158	1385	2336	2643	2075	21	28	2717	2370	2551	3583	2792	2951	2538	3057	3075	3411	2887	3610
PC(P-36:1)	697525	868767	723734	830591	807316	818075	610979	789866	662557	1002466	722203	1866581	789823	1059939	830610	1097093	1596334	993246	678250	702009	610100	683404	606307	571294	681875	603297	514591	539737	509585	729533	535456	664139	564843	647304	764763	561137	579691	437865	640516	592755	642175	598429	550203	644840	7521	9273	603482	590168	625260	611460	654869	674561	704508	678471	693850	700151	687361	757641
PC(P-36:2)	170275	207467	173556	225782	201130	212863	195461	201289	206515	284307	201610	479011	230947	303906	294719	316244	448403	249835	237177	237315	207656	252685	101199	105477	132391	122264	114647	107856	149149	132257	129101	134550	129511	168571	217625	138052	151214	125491	167011	154577	152307	153056	157299	167132	1317	1257	148150	154872	155084	145934	153457	160208	149892	148339	180271	169316	175521	193519
PC(P-36:3)	208162	205575	215164	263982	232217	288695	195337	200164	208455	234498	184243	408273	224884	258465	182731	258198	287761	241427	212307	189252	215425	211286	142615	139689	144285	150619	127639	133136	204091	151552	147104	113427	134555	207741	188427	156374	141405	148979	193090	197448	181125	170807	166887	153565	648	474	154441	154296	166934	138267	152734	165846	184746	172496	168511	189547	175972	176483
PC(P-36:4)	294494	338114	273151	348401	377858	316971	301932	287862	377948	369301	287556	262027	242854	218699	196289	253488	158726	172914	262182	219840	270196	246555	369374	317409	341905	335043	260747	388424	393889	320007	263397	274068	305114	285815	324976	438471	385308	509116	369733	460983	501821	358310	311412	272876	20	192	316165	339422	321095	338304	323306	392704	339856	360762	358723	367549	386579	370622
PC(P-36:5)	35695	40856	26309	50701	41539	40091	35481	33643	34666	43444	35159	23501	37357	22136	35471	44838	21465	21638	33254	34117	38455	46498	14688	11278	14838	10979	8124	10904	12361	15674	11091	10840	11850	14122	17446	14347	13387	15081	13498	17327	17064	11593	13490	12891	83	45	18058	20117	19814	18450	17403	18651	21251	21058	19025	22919	18620	24614
PC(P-38:0)	45833	67683	56207	85746	51219	59790	51676	57326	42053	47631	49225	75076	61992	71716	74788	69424	73602	62331	63195	47734	60797	49874	12941	14260	15796	14792	15988	15683	19870	19375	20485	17082	15455	17939	27888	16990	18578	14046	20935	18956	16460	22674	20183	17817	148	92	24272	23483	22304	23816	25432	25058	27640	26341	29358	34443	27607	29013
PC(P-38:1)	255435	322556	338904	442832	266220	374322	265423	243179	212533	326594	269511	281553	219993	292142	277888	267277	290287	282790	269551	211126	205035	226773	70840	76622	85029	78256	79029	69929	87853	111645	82098	92488	76878	93210	134662	89823	89319	61307	114561	111916	102049	100540	86101	91917	957	NA	128194	133473	137939	134780	146104	143271	146049	154166	154918	168291	146344	149153
PC(P-38:2)	86971	112711	110394	99156	102479	96602	98158	112524	108957	112586	98041	224259	116073	171609	124812	166125	244380	175398	102847	107055	116555	109737	88555	74911	80401	73966	71977	66562	78724	91470	78184	80912	75640	92031	112838	83578	78359	72997	90900	95681	91189	88980	82657	95305	114	498	74772	82985	83242	85239	79248	85361	87050	82973	84693	93980	87204	95351
PC(P-38:3)	203457	219788	194540	199187	207702	233713	180413	226782	176147	264856	168732	545834	222570	294657	205995	320016	367821	274217	196657	168535	172670	189832	213121	213468	222972	224264	188079	191225	246287	262092	218541	207651	191998	246584	290098	183579	176011	182137	254464	203469	201486	232670	240765	195542	1548	1520	193141	209397	173505	194773	179718	212515	195772	219593	201768	226818	216836	233690
PC(P-38:4)	384178	377370	361266	421472	490691	529660	386414	385341	421032	451868	385926	427430	334929	354940	342947	433437	305186	295823	345127	296911	344040	309019	442296	472053	393812	448882	345522	456489	549766	451100	371478	365017	354344	425869	419622	369996	420451	325399	393405	429281	319156	347819	415826	323338	1146	807	355053	335026	314755	349235	345345	368272	392352	384540	371341	427426	427008	397317
PC(P-38:5)	373185	364609	270772	375530	361609	386151	351232	313015	317267	347346	287149	586989	311095	395824	300637	410441	495708	334200	294713	284841	311207	297337	223364	237528	223838	246034	148034	228152	232412	231694	194734	177009	198790	245766	261566	197866	230971	189398	242115	249617	218104	199898	211023	192060	598	526	221138	232342	237869	256866	226532	232070	262854	246208	270787	246592	258782	264054
PC(P-38:6)	4256	4311	3888	6482	4921	4484	3624	5007	4716	5902	3705	2163	2856	2871	3002	3308	1995	3021	2997	2535	3059	2445	3315	2716	3008	2828	1636	2670	2661	3283	2871	3178	3154	2455	3019	3094	2440	3186	2739	2818	3210	2173	2556	2360	0	5	2649	3170	2955	2510	2552	2938	3530	2409	2799	3457	4268	3966
PC(P-40:0)	591640	614754	520465	559198	579140	440767	493715	643305	513181	624505	543404	457501	505293	589068	540652	555975	374369	437110	564048	518843	529100	488101	1524266	1369935	1604701	1321612	1356571	2243613	1250682	1501498	1259615	1156057	1429505	856985	1055808	1151719	884913	1079291	980793	970825	1169960	811962	1052358	828608	487	607	901795	820798	1064265	970873	759702	913766	1082493	864847	1002681	1075704	1124183	1004749
PC(P-40:1)	64817	75751	45900	65020	58000	57970	57986	64170	65574	84670	71084	60886	48403	53748	65896	52808	46131	50167	57693	56291	59076	61913	64728	53394	82602	65604	57542	84443	36023	74205	50408	53606	76106	56411	73353	65805	52217	69287	63192	52691	82157	63548	51959	47685	245	45	59911	50665	51519	54310	58073	58593	59238	57271	63022	53151	61106	59639
PC(P-40:2)	36904	41840	37156	43200	41147	42782	37135	41656	36614	44939	34644	40190	43531	39282	38875	40886	43922	41556	42159	35643	37271	38485	22965	21998	23274	21406	17006	16256	21717	23958	23711	22564	18912	24006	30630	19826	17527	15636	24083	24426	21827	20892	22730	20014	34	704	23562	19496	22472	21023	23530	21723	26457	24593	26886	27136	30257	26634
PC(P-40:3)	88623	102760	124428	119014	107074	112538	93565	110123	114605	152137	89024	110756	76154	95984	88448	88957	77097	87352	93637	74686	79094	77088	88469	108192	90098	94154	74432	73843	118931	106244	101924	88513	82581	100476	115724	76500	86224	69703	105978	96676	94837	113008	90614	86264	654	1252	80015	71298	77616	74973	83701	82756	88443	91081	90293	87245	97279	104209
PC(P-40:4)	91515	100703	96076	105653	115548	105505	96394	105466	115232	116681	83413	200062	92634	128354	97519	137242	148546	128064	87616	87147	96285	88290	102856	112132	96171	104733	92478	115104	111601	99398	92401	100102	84822	105217	125257	90126	87106	74111	93045	100069	97478	83648	104700	91285	646	425	86997	86289	80874	91135	80274	79061	95235	101605	95280	101404	103021	96832
PC(P-40:5)	274241	304911	235911	340636	337533	345393	345071	289344	287276	338877	266557	738198	332892	514389	328329	516911	706578	411704	274211	291343	308804	288934	244059	252386	241064	259847	176253	219516	215455	286031	207297	206554	224073	278683	372043	217482	261984	186085	289209	245543	268344	227650	289172	283155	351	1121	243946	223051	242249	230500	236064	212710	243542	260530	275382	261736	297906	279956
PC(P-40:6)	498422	473894	425238	614191	540125	443501	446668	523712	518061	542763	449171	432788	361250	403691	405378	458910	291856	319392	419430	359937	421775	371592	442821	488307	422020	506812	310992	437057	387839	484799	328805	391401	411365	370446	504942	375622	419920	343756	417779	444788	479180	335687	432768	399905	237	946	367598	336690	413255	367134	406701	344860	433154	428612	431857	461404	520770	470118
PC 15:0/18:1(d7)	2255225	2282772	2456303	2196114	1991054	1918726	2166109	2689280	1883015	1911299	2019282	2467845	2526041	2869455	2784730	2279189	2978723	3208680	2367059	2543834	2155888	2333410	1605379	1630844	1697751	1583876	1387168	1440288	2091602	1677586	1666250	1652766	1245912	2114906	1899888	1356626	1579128	1790619	3122693	1839624	1977808	2185684	2321410	2066063	934	819	5225	4853	7417	4181	5221	6443	13206	7149	5478	9810	9496	7489
MS_METABOLITE_DATA_END
#METABOLITES
METABOLITES_START
metabolite_name
LPC 18:1(d7)
LPC 14:0
LPC 16:0
LPC 16:1
LPC 18:0
LPC 18:1
LPC 18:2
LPC 20:0
LPC 20:1
LPC 20:2
LPC 20:3
LPC 20:4
LPC 20:5
LPC 22:0
LPC 22:1
LPC 22:2
LPC 22:3
LPC 22:4
LPC 22:5
LPC 22:6
LPC 24:0
LPC 24:1
LPC 26:0
LPE 18:1(d7)
LPE 16:0
LPE 16:1
LPE 18:0
LPE 18:1
LPE 18:2
LPE 20:0
LPE 20:1
LPE 20:2
LPE 20:3
LPE 20:4
LPE 20:5
LPE 22:4
LPE 22:5
LPE 22:6
PC(O-32:0)
PC(O-34:1)
PC(O-34:2)
PC(O-34:3)
PC(O-36:2)
PC(O-36:3)
PC(O-36:4)
PC(O-36:5)
PC(O-38:0)
PC(O-38:2)
PC(O-38:3)
PC(O-38:4)
PC(O-38:5)
PC(O-40:0)
PC(O-40:1)
PC(O-40:2)
PC(O-40:3)
PC(O-40:4)
PC(O-40:5)
PC(O-40:6)
PC(O-40:7)
PC(P-32:0)
PC(P-34:1)
PC(P-34:2)
PC(P-34:3)
PC(P-36:1)
PC(P-36:2)
PC(P-36:3)
PC(P-36:4)
PC(P-36:5)
PC(P-38:0)
PC(P-38:1)
PC(P-38:2)
PC(P-38:3)
PC(P-38:4)
PC(P-38:5)
PC(P-38:6)
PC(P-40:0)
PC(P-40:1)
PC(P-40:2)
PC(P-40:3)
PC(P-40:4)
PC(P-40:5)
PC(P-40:6)
PC 15:0/18:1(d7)
METABOLITES_END
#END