#METABOLOMICS WORKBENCH Codreags00_20200327_075634 DATATRACK_ID:1959 STUDY_ID:ST001333 ANALYSIS_ID:AN002222 PROJECT_ID:PR000910
VERSION             	1
CREATED_ON             	March 30, 2020, 10:57 am
#PROJECT
PR:PROJECT_TITLE                 	Huntington’s Disease Genotype Suppresses Global Manganese-Responsive Processes
PR:PROJECT_TYPE                  	Untargeted Metabolomics analysis
PR:PROJECT_SUMMARY               	Global untargeted metabolomics studies performed in the striatum tissue, the
PR:PROJECT_SUMMARY               	brain region most sensitive to neurodegeneration in Huntington’s Disease, to
PR:PROJECT_SUMMARY               	investigate global Mn-dependent and Mn-responsive biology following various Mn
PR:PROJECT_SUMMARY               	exposures in a mouse model of HD.
PR:INSTITUTE                     	Vanderbilt University
PR:DEPARTMENT                    	Chemistry
PR:LABORATORY                    	Center for Innovative Technology
PR:LAST_NAME                     	Codreanu
PR:FIRST_NAME                    	Simona
PR:ADDRESS                       	1234 Stevenson Center Lane
PR:EMAIL                         	simona.codreanu@vanderbilt.edu
PR:PHONE                         	6158758422
#STUDY
ST:STUDY_TITLE                   	Huntington’s Disease Genotype Suppresses Global Manganese-Responsive Processes
ST:STUDY_TYPE                    	Untargeted metabolomics analysis
ST:STUDY_SUMMARY                 	Global untargeted metabolomics studies performed in the striatum tissue, the
ST:STUDY_SUMMARY                 	brain region most sensitive to neurodegeneration in Huntington’s Disease, to
ST:STUDY_SUMMARY                 	investigate global Mn-dependent and Mn-responsive biology following various Mn
ST:STUDY_SUMMARY                 	exposures in a mouse model of HD.
ST:INSTITUTE                     	Vanderbilt University
ST:DEPARTMENT                    	Chemistry
ST:LABORATORY                    	Center for Innovative Technology
ST:LAST_NAME                     	Codreanu
ST:FIRST_NAME                    	Simona
ST:ADDRESS                       	1234 Stevenson Center Lane
ST:EMAIL                         	simona.codreanu@vanderbilt.edu
ST:PHONE                         	6158758422
#SUBJECT
SU:SUBJECT_TYPE                  	Other
SU:SUBJECT_SPECIES               	Mus musculus
SU:TAXONOMY_ID                   	10090
SU:GENOTYPE_STRAIN               	YAC128 and wildtype (WT)
SU:AGE_OR_AGE_RANGE              	12 week and 32 week
SU:GENDER                        	Male and female
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data
SUBJECT_SAMPLE_FACTORS           	1796	41_1796_C_M_128_0	Sex:M | Genotype:128 | Treatment Dosage:-	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP_41_1796_C_M_128_0
SUBJECT_SAMPLE_FACTORS           	1807	89_1807_C_M_WT_5	Sex:M | Genotype:WT | Treatment Dosage:5	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__89_1807_C_M_WT_5
SUBJECT_SAMPLE_FACTORS           	1808	23_1808_C_M_WT_15	Sex:M | Genotype:WT | Treatment Dosage:15	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__23_1808_C_M_WT_15
SUBJECT_SAMPLE_FACTORS           	1809	26_1809_C_M_128_15	Sex:M | Genotype:128 | Treatment Dosage:15	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__26_1809_C_M_128_15
SUBJECT_SAMPLE_FACTORS           	1811	30_1811_C_M_128_50	Sex:M | Genotype:128 | Treatment Dosage:50	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__30_1811_C_M_128_50
SUBJECT_SAMPLE_FACTORS           	1813	95_1813_C_M_WT_50	Sex:M | Genotype:WT | Treatment Dosage:50	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__95_1813_C_M_WT_50
SUBJECT_SAMPLE_FACTORS           	1814	113_1814_C_M_WT_5	Sex:M | Genotype:WT | Treatment Dosage:5	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__113_1814_C_M_WT_5
SUBJECT_SAMPLE_FACTORS           	1815	77_1815_C_M_WT_0	Sex:M | Genotype:WT | Treatment Dosage:-	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__77_1815_C_M_WT_0
SUBJECT_SAMPLE_FACTORS           	1816	38_1816_C_M_WT_15	Sex:M | Genotype:WT | Treatment Dosage:15	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__38_1816_C_M_WT_15
SUBJECT_SAMPLE_FACTORS           	1817	91_1817_C_M_WT_15	Sex:M | Genotype:WT | Treatment Dosage:15	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__91_1817_C_M_WT_15
SUBJECT_SAMPLE_FACTORS           	1818	110_1818_C_M_128_5	Sex:M | Genotype:128 | Treatment Dosage:5	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__110_1818_C_M_128_5
SUBJECT_SAMPLE_FACTORS           	1821	131_1821_C_M_WT_0	Sex:M | Genotype:WT | Treatment Dosage:-	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__131_1821_C_M_WT_0
SUBJECT_SAMPLE_FACTORS           	1822	76_1822_C_M_128_0	Sex:M | Genotype:128 | Treatment Dosage:-	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__76_1822_C_M_128_0
SUBJECT_SAMPLE_FACTORS           	1823	128_1823_C_M_WT_5	Sex:M | Genotype:WT | Treatment Dosage:5	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__128_1823_C_M_WT_5
SUBJECT_SAMPLE_FACTORS           	1824	129_1824_C_M_WT_15	Sex:M | Genotype:WT | Treatment Dosage:15	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__129_1824_C_M_WT_15
SUBJECT_SAMPLE_FACTORS           	1825	84_1825_C_M_WT_50	Sex:M | Genotype:WT | Treatment Dosage:50	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__84_1825_C_M_WT_50
SUBJECT_SAMPLE_FACTORS           	1827	133_1827_C_F_WT_5	Sex:F | Genotype:WT | Treatment Dosage:5	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__133_1827_C_F_WT_5
SUBJECT_SAMPLE_FACTORS           	1828	79_1828_C_F_WT_15	Sex:F | Genotype:WT | Treatment Dosage:15	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__79_1828_C_F_WT_15
SUBJECT_SAMPLE_FACTORS           	1830	66_1830_C_M_128_15	Sex:M | Genotype:128 | Treatment Dosage:15	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__66_1830_C_M_128_15
SUBJECT_SAMPLE_FACTORS           	1831	82_1831_C_M_128_50	Sex:M | Genotype:128 | Treatment Dosage:50	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__82_1831_C_M_128_50
SUBJECT_SAMPLE_FACTORS           	1832	107_1832_C_M_WT_0	Sex:M | Genotype:WT | Treatment Dosage:-	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__107_1832_C_M_WT_0
SUBJECT_SAMPLE_FACTORS           	1833	100_1833_C_F_128_0	Sex:F | Genotype:128 | Treatment Dosage:-	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__100_1833_C_F_128_0
SUBJECT_SAMPLE_FACTORS           	1834	69_1834_C_F_WT_50	Sex:F | Genotype:WT | Treatment Dosage:50	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__69_1834_C_F_WT_50
SUBJECT_SAMPLE_FACTORS           	1837	61_1837_C_M_128_0	Sex:M | Genotype:128 | Treatment Dosage:-	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__61_1837_C_M_128_0
SUBJECT_SAMPLE_FACTORS           	1838	5_1838_C_M_WT_15	Sex:M | Genotype:WT | Treatment Dosage:15	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__5_1838_C_M_WT_15
SUBJECT_SAMPLE_FACTORS           	1839	130_1839_C_M_WT_50	Sex:M | Genotype:WT | Treatment Dosage:50	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__130_1839_C_M_WT_50
SUBJECT_SAMPLE_FACTORS           	1840	21_1840_A_M_WT_50	Sex:M | Genotype:WT | Treatment Dosage:50	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__21_1840_A_M_WT_50
SUBJECT_SAMPLE_FACTORS           	1841	1_1841_C_F_128_5	Sex:F | Genotype:128 | Treatment Dosage:5	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__1_1841_C_F_128_5
SUBJECT_SAMPLE_FACTORS           	1842	68_1842_C_F_WT_15	Sex:F | Genotype:WT | Treatment Dosage:15	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__68_1842_C_F_WT_15
SUBJECT_SAMPLE_FACTORS           	1843	127_1843_C_F_WT_5	Sex:F | Genotype:WT | Treatment Dosage:5	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__127_1843_C_F_WT_5
SUBJECT_SAMPLE_FACTORS           	1843b	124_1843b_C_F_128_15	Sex:F | Genotype:128 | Treatment Dosage:15	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__124_1843b_C_F_128_15
SUBJECT_SAMPLE_FACTORS           	1845	56_1845_C_M_128_5	Sex:M | Genotype:128 | Treatment Dosage:5	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__56_1845_C_M_128_5
SUBJECT_SAMPLE_FACTORS           	1847	111_1847_C_F_128_50	Sex:F | Genotype:128 | Treatment Dosage:50	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__111_1847_C_F_128_50
SUBJECT_SAMPLE_FACTORS           	1848	22_1848_C_F_WT_15	Sex:F | Genotype:WT | Treatment Dosage:15	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__22_1848_C_F_WT_15
SUBJECT_SAMPLE_FACTORS           	1849	55_1849_C_F_WT_0	Sex:F | Genotype:WT | Treatment Dosage:-	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__55_1849_C_F_WT_0
SUBJECT_SAMPLE_FACTORS           	1850	50_1850_C_F_WT_50	Sex:F | Genotype:WT | Treatment Dosage:50	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__50_1850_C_F_WT_50
SUBJECT_SAMPLE_FACTORS           	1852	32_1852_C_M_WT_5	Sex:M | Genotype:WT | Treatment Dosage:5	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__32_1852_C_M_WT_5
SUBJECT_SAMPLE_FACTORS           	1855	94_1855_C_F_WT_5	Sex:F | Genotype:WT | Treatment Dosage:5	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__94_1855_C_F_WT_5
SUBJECT_SAMPLE_FACTORS           	1856	28_1856_C_F_128_0	Sex:F | Genotype:128 | Treatment Dosage:-	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__28_1856_C_F_128_0
SUBJECT_SAMPLE_FACTORS           	1857	135_1857_C_F_128_5	Sex:F | Genotype:128 | Treatment Dosage:5	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__135_1857_C_F_128_5
SUBJECT_SAMPLE_FACTORS           	1858	64_1858_C_M_WT_50	Sex:M | Genotype:WT | Treatment Dosage:50	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__64_1858_C_M_WT_50
SUBJECT_SAMPLE_FACTORS           	1860	102_1860_C_M_WT_0	Sex:M | Genotype:WT | Treatment Dosage:-	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__102_1860_C_M_WT_0
SUBJECT_SAMPLE_FACTORS           	1861	112_1861_C_M_128_50	Sex:M | Genotype:128 | Treatment Dosage:50	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__112_1861_C_M_128_50
SUBJECT_SAMPLE_FACTORS           	1862	125_1862_C_M_WT_5	Sex:M | Genotype:WT | Treatment Dosage:5	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__125_1862_C_M_WT_5
SUBJECT_SAMPLE_FACTORS           	1864	73_1864_C_F_128_15	Sex:F | Genotype:128 | Treatment Dosage:15	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__73_1864_C_F_128_15
SUBJECT_SAMPLE_FACTORS           	1865	86_1865_C_F_WT_15	Sex:F | Genotype:WT | Treatment Dosage:15	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__86_1865_C_F_WT_15
SUBJECT_SAMPLE_FACTORS           	1866	78_1866_C_F_WT_50	Sex:F | Genotype:WT | Treatment Dosage:50	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__78_1866_C_F_WT_50
SUBJECT_SAMPLE_FACTORS           	1867	123_1867_C_F_128_50	Sex:F | Genotype:128 | Treatment Dosage:50	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__123_1867_C_F_128_50
SUBJECT_SAMPLE_FACTORS           	1869	80_1869_C_F_WT_0	Sex:F | Genotype:WT | Treatment Dosage:-	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__80_1869_C_F_WT_0
SUBJECT_SAMPLE_FACTORS           	1871	54_1871_C_M_128_5	Sex:M | Genotype:128 | Treatment Dosage:5	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__54_1871_C_M_128_5
SUBJECT_SAMPLE_FACTORS           	1872	59_1872_C_M_WT_50	Sex:M | Genotype:WT | Treatment Dosage:50	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__59_1872_C_M_WT_50
SUBJECT_SAMPLE_FACTORS           	1874	104_1874_C_F_128_0	Sex:F | Genotype:128 | Treatment Dosage:-	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__104_1874_C_F_128_0
SUBJECT_SAMPLE_FACTORS           	1875	87_1875_C_F_WT_5	Sex:F | Genotype:WT | Treatment Dosage:5	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__87_1875_C_F_WT_5
SUBJECT_SAMPLE_FACTORS           	1876	37_1876_C_F_WT_15	Sex:F | Genotype:WT | Treatment Dosage:15	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__37_1876_C_F_WT_15
SUBJECT_SAMPLE_FACTORS           	1877	85_1877_C_F_128_5	Sex:F | Genotype:128 | Treatment Dosage:5	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__85_1877_C_F_128_5
SUBJECT_SAMPLE_FACTORS           	1878	108_1878_C_F_WT_50	Sex:F | Genotype:WT | Treatment Dosage:50	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__108_1878_C_F_WT_50
SUBJECT_SAMPLE_FACTORS           	1879	116_1879_C_F_128_15	Sex:F | Genotype:128 | Treatment Dosage:15	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__116_1879_C_F_128_15
SUBJECT_SAMPLE_FACTORS           	1884	33_1884_C_F_WT_50	Sex:F | Genotype:WT | Treatment Dosage:50	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__33_1884_C_F_WT_50
SUBJECT_SAMPLE_FACTORS           	1885	114_1885_C_F_128_5	Sex:F | Genotype:128 | Treatment Dosage:5	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__114_1885_C_F_128_5
SUBJECT_SAMPLE_FACTORS           	1886	93_1886_C_F_WT_5	Sex:F | Genotype:WT | Treatment Dosage:5	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__93_1886_C_F_WT_5
SUBJECT_SAMPLE_FACTORS           	1887	117_1887_C_F_WT_0	Sex:F | Genotype:WT | Treatment Dosage:-	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__117_1887_C_F_WT_0
SUBJECT_SAMPLE_FACTORS           	1891	39_1891_C_F_128_50	Sex:F | Genotype:128 | Treatment Dosage:50	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__39_1891_C_F_128_50
SUBJECT_SAMPLE_FACTORS           	1892	99_1892_A_M_WT_0	Sex:M | Genotype:WT | Treatment Dosage:-	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__99_1892_A_M_WT_0
SUBJECT_SAMPLE_FACTORS           	1893	90_1893_C_F_128_0	Sex:F | Genotype:128 | Treatment Dosage:-	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__90_1893_C_F_128_0
SUBJECT_SAMPLE_FACTORS           	1894	75_1894_A_F_WT_0	Sex:F | Genotype:WT | Treatment Dosage:-	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__75_1894_A_F_WT_0
SUBJECT_SAMPLE_FACTORS           	1895	47_1895_C_M_WT_15	Sex:M | Genotype:WT | Treatment Dosage:15	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__47_1895_C_M_WT_15
SUBJECT_SAMPLE_FACTORS           	1896	45_1896_C_M_128_15	Sex:M | Genotype:128 | Treatment Dosage:15	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__45_1896_C_M_128_15
SUBJECT_SAMPLE_FACTORS           	1898	57_1898_A_M_WT_50	Sex:M | Genotype:WT | Treatment Dosage:50	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__57_1898_A_M_WT_50
SUBJECT_SAMPLE_FACTORS           	1899	132_1899_C_M_128_50	Sex:M | Genotype:128 | Treatment Dosage:50	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__132_1899_C_M_128_50
SUBJECT_SAMPLE_FACTORS           	1901	72_1901_C_M_128_5	Sex:M | Genotype:128 | Treatment Dosage:5	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__72_1901_C_M_128_5
SUBJECT_SAMPLE_FACTORS           	1903	101_1903_C_M_128_0	Sex:M | Genotype:128 | Treatment Dosage:-	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__101_1903_C_M_128_0
SUBJECT_SAMPLE_FACTORS           	1904	58_1904_C_M_128_15	Sex:M | Genotype:128 | Treatment Dosage:15	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__58_1904_C_M_128_15
SUBJECT_SAMPLE_FACTORS           	1905	24_1905_A_F_WT_0	Sex:F | Genotype:WT | Treatment Dosage:-	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__24_1905_A_F_WT_0
SUBJECT_SAMPLE_FACTORS           	1906	3_1906_A_F_WT_50	Sex:F | Genotype:WT | Treatment Dosage:50	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__3_1906_A_F_WT_50
SUBJECT_SAMPLE_FACTORS           	1907	42_1907_C_F_128_5	Sex:F | Genotype:128 | Treatment Dosage:5	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__42_1907_C_F_128_5
SUBJECT_SAMPLE_FACTORS           	1908	49_1908_A_F_WT_0	Sex:F | Genotype:WT | Treatment Dosage:-	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__49_1908_A_F_WT_0
SUBJECT_SAMPLE_FACTORS           	1909	7_1909_C_F_128_15	Sex:F | Genotype:128 | Treatment Dosage:15	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__7_1909_C_F_128_15
SUBJECT_SAMPLE_FACTORS           	1910	4_1910_A_F_WT_50	Sex:F | Genotype:WT | Treatment Dosage:50	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__4_1910_A_F_WT_50
SUBJECT_SAMPLE_FACTORS           	1911	136_1911_A_M_WT_0	Sex:M | Genotype:WT | Treatment Dosage:-	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__136_1911_A_M_WT_0
SUBJECT_SAMPLE_FACTORS           	1912	96_1912_A_M_WT_50	Sex:M | Genotype:WT | Treatment Dosage:50	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__96_1912_A_M_WT_50
SUBJECT_SAMPLE_FACTORS           	1913	20_1913_A_M_WT_0	Sex:M | Genotype:WT | Treatment Dosage:-	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__20_1913_A_M_WT_0
SUBJECT_SAMPLE_FACTORS           	1914	46_1914_C_M_WT_0	Sex:M | Genotype:WT | Treatment Dosage:-	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__46_1914_C_M_WT_0
SUBJECT_SAMPLE_FACTORS           	1915	70_1915_C_M_128_50	Sex:M | Genotype:128 | Treatment Dosage:50	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__70_1915_C_M_128_50
SUBJECT_SAMPLE_FACTORS           	1916	43_1916_A_M_WT_50	Sex:M | Genotype:WT | Treatment Dosage:50	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__43_1916_A_M_WT_50
SUBJECT_SAMPLE_FACTORS           	1917	71_1917_A_M_128_0	Sex:M | Genotype:128 | Treatment Dosage:-	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__71_1917_A_M_128_0
SUBJECT_SAMPLE_FACTORS           	1918	115_1918_A_F_WT_50	Sex:F | Genotype:WT | Treatment Dosage:50	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__115_1918_A_F_WT_50
SUBJECT_SAMPLE_FACTORS           	1919	88_1919_C_F_128_0	Sex:F | Genotype:128 | Treatment Dosage:-	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__88_1919_C_F_128_0
SUBJECT_SAMPLE_FACTORS           	1920	62_1920_C_F_128_5	Sex:F | Genotype:128 | Treatment Dosage:5	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__62_1920_C_F_128_5
SUBJECT_SAMPLE_FACTORS           	1921	10_1921_C_F_128_15	Sex:F | Genotype:128 | Treatment Dosage:15	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__10_1921_C_F_128_15
SUBJECT_SAMPLE_FACTORS           	1922	122_1922_A_F_WT_0	Sex:F | Genotype:WT | Treatment Dosage:-	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__122_1922_A_F_WT_0
SUBJECT_SAMPLE_FACTORS           	1923	60_1923_C_F_128_50	Sex:F | Genotype:128 | Treatment Dosage:50	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__60_1923_C_F_128_50
SUBJECT_SAMPLE_FACTORS           	1924	27_1924_C_M_WT_0	Sex:M | Genotype:WT | Treatment Dosage:-	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__27_1924_C_M_WT_0
SUBJECT_SAMPLE_FACTORS           	1928	134_1928_A_F_128_0	Sex:F | Genotype:128 | Treatment Dosage:-	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__134_1928_A_F_128_0
SUBJECT_SAMPLE_FACTORS           	1929	25_1929_A_F_WT_50	Sex:F | Genotype:WT | Treatment Dosage:50	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__25_1929_A_F_WT_50
SUBJECT_SAMPLE_FACTORS           	1930	65_1930_C_M_128_15	Sex:M | Genotype:128 | Treatment Dosage:15	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__65_1930_C_M_128_15
SUBJECT_SAMPLE_FACTORS           	1931	6_1931_C_M_WT_15	Sex:M | Genotype:WT | Treatment Dosage:15	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__6_1931_C_M_WT_15
SUBJECT_SAMPLE_FACTORS           	1933	119_1933_C_F_WT_0	Sex:F | Genotype:WT | Treatment Dosage:-	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__119_1933_C_F_WT_0
SUBJECT_SAMPLE_FACTORS           	1935	74_1935_A_M_WT_0	Sex:M | Genotype:WT | Treatment Dosage:-	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__74_1935_A_M_WT_0
SUBJECT_SAMPLE_FACTORS           	1936	29_1936_A_M_WT_50	Sex:M | Genotype:WT | Treatment Dosage:50	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__29_1936_A_M_WT_50
SUBJECT_SAMPLE_FACTORS           	1937	92_1937_A_F_128_50	Sex:F | Genotype:128 | Treatment Dosage:50	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__92_1937_A_F_128_50
SUBJECT_SAMPLE_FACTORS           	1938	97_1938_A_M_128_50	Sex:M | Genotype:128 | Treatment Dosage:50	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__97_1938_A_M_128_50
SUBJECT_SAMPLE_FACTORS           	1939	105_1939_A_F_128_50	Sex:F | Genotype:128 | Treatment Dosage:50	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__105_1939_A_F_128_50
SUBJECT_SAMPLE_FACTORS           	1943	121_1943_A_F_WT_50	Sex:F | Genotype:WT | Treatment Dosage:50	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__121_1943_A_F_WT_50
SUBJECT_SAMPLE_FACTORS           	1944	31_1944_A_M_WT_0	Sex:M | Genotype:WT | Treatment Dosage:-	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__31_1944_A_M_WT_0
SUBJECT_SAMPLE_FACTORS           	1945	137_1945_A_M_128_0	Sex:M | Genotype:128 | Treatment Dosage:-	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__137_1945_A_M_128_0
SUBJECT_SAMPLE_FACTORS           	1946	138_1946_A_M_WT_50	Sex:M | Genotype:WT | Treatment Dosage:50	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__138_1946_A_M_WT_50
SUBJECT_SAMPLE_FACTORS           	1948	139_1948_A_M_WT_0	Sex:M | Genotype:WT | Treatment Dosage:-	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__139_1948_A_M_WT_0
SUBJECT_SAMPLE_FACTORS           	1949	140_1949_A_F_128_0	Sex:F | Genotype:128 | Treatment Dosage:-	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__140_1949_A_F_128_0
SUBJECT_SAMPLE_FACTORS           	1950	141_1950_A_F_128_50	Sex:F | Genotype:128 | Treatment Dosage:50	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__141_1950_A_F_128_50
SUBJECT_SAMPLE_FACTORS           	1951	48_1951_A_F_WT_0	Sex:F | Genotype:WT | Treatment Dosage:-	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__48_1951_A_F_WT_0
SUBJECT_SAMPLE_FACTORS           	1953	103_1953_A_M_128_50	Sex:M | Genotype:128 | Treatment Dosage:50	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__103_1953_A_M_128_50
SUBJECT_SAMPLE_FACTORS           	1954	126_1954_C_F_128_0	Sex:F | Genotype:128 | Treatment Dosage:-	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__126_1954_C_F_128_0
SUBJECT_SAMPLE_FACTORS           	1955	52_1955_C_F_WT_0	Sex:F | Genotype:WT | Treatment Dosage:-	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__106_1956_C_M_WT_0
SUBJECT_SAMPLE_FACTORS           	1957	35_1957_C_M_WT_5	Sex:M | Genotype:WT | Treatment Dosage:5	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__35_1957_C_M_WT_5
SUBJECT_SAMPLE_FACTORS           	1958	8_1958_C_M_128_5	Sex:M | Genotype:128 | Treatment Dosage:5	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__8_1958_C_M_128_5
SUBJECT_SAMPLE_FACTORS           	1959	118_1959_C_M_WT_15	Sex:M | Genotype:WT | Treatment Dosage:15	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__118_1959_C_M_WT_15
SUBJECT_SAMPLE_FACTORS           	1960	63_1960_C_M_WT_50	Sex:M | Genotype:WT | Treatment Dosage:50	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__63_1960_C_M_WT_50
SUBJECT_SAMPLE_FACTORS           	1961	120_1961_A_M_WT_0	Sex:M | Genotype:WT | Treatment Dosage:-	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__120_1961_A_M_WT_0
SUBJECT_SAMPLE_FACTORS           	1963	40_1963_C_F_128_5	Sex:F | Genotype:128 | Treatment Dosage:5	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__40_1963_C_F_128_5
SUBJECT_SAMPLE_FACTORS           	1964	9_1964_C_F_128_15	Sex:F | Genotype:128 | Treatment Dosage:15	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__9_1964_C_F_128_15
SUBJECT_SAMPLE_FACTORS           	1965	36_1965_C_F_128_50	Sex:F | Genotype:128 | Treatment Dosage:50	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__36_1965_C_F_128_50
SUBJECT_SAMPLE_FACTORS           	1966	14_1966_A_M_128_0	Sex:M | Genotype:128 | Treatment Dosage:-	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__14_1966_A_M_128_0
SUBJECT_SAMPLE_FACTORS           	1967	15_1967_A_M_128_50	Sex:M | Genotype:128 | Treatment Dosage:50	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__15_1967_A_M_128_50
SUBJECT_SAMPLE_FACTORS           	1970	81_1970_A_M_WT_50	Sex:M | Genotype:WT | Treatment Dosage:50	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__81_1970_A_M_WT_50
SUBJECT_SAMPLE_FACTORS           	1971	13_1971_A_M_WT_50	Sex:M | Genotype:WT | Treatment Dosage:50	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__13_1971_A_M_WT_50
SUBJECT_SAMPLE_FACTORS           	1972	98_1972_A_M_128_0	Sex:M | Genotype:128 | Treatment Dosage:-	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__98_1972_A_M_128_0
SUBJECT_SAMPLE_FACTORS           	1973	51_1973_A_M_WT_0	Sex:M | Genotype:WT | Treatment Dosage:-	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__51_1973_A_M_WT_0
SUBJECT_SAMPLE_FACTORS           	1974	12_1974_A_M_128_50	Sex:M | Genotype:128 | Treatment Dosage:50	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__12_1974_A_M_128_50
SUBJECT_SAMPLE_FACTORS           	1976	18_1976_A_F_128_0	Sex:F | Genotype:128 | Treatment Dosage:-	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__18_1976_A_F_128_0
SUBJECT_SAMPLE_FACTORS           	1978	67_1978_A_M_128_50	Sex:M | Genotype:128 | Treatment Dosage:50	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__67_1978_A_M_128_50
SUBJECT_SAMPLE_FACTORS           	1979	83_1979_A_M_WT_50	Sex:M | Genotype:WT | Treatment Dosage:50	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__83_1979_A_M_WT_50
SUBJECT_SAMPLE_FACTORS           	1980	109_1980_A_M_128_0	Sex:M | Genotype:128 | Treatment Dosage:-	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__109_1980_A_M_128_0
SUBJECT_SAMPLE_FACTORS           	1981	53_1981_A_M_WT_0	Sex:M | Genotype:WT | Treatment Dosage:-	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__53_1981_A_M_WT_0
SUBJECT_SAMPLE_FACTORS           	1982	2_1982_A_M_128_50	Sex:M | Genotype:128 | Treatment Dosage:50	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__2_1982_A_M_128_50
SUBJECT_SAMPLE_FACTORS           	1983	17_1983_A_M_128_0	Sex:M | Genotype:128 | Treatment Dosage:-	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__17_1983_A_M_128_0
SUBJECT_SAMPLE_FACTORS           	1984	11_1984_A_F_128_0	Sex:F | Genotype:128 | Treatment Dosage:-	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__11_1984_A_F_128_0
SUBJECT_SAMPLE_FACTORS           	1985	19_1985_A_F_128_50	Sex:F | Genotype:128 | Treatment Dosage:50	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__19_1985_A_F_128_50
SUBJECT_SAMPLE_FACTORS           	1986	16_1986_A_F_128_0	Sex:F | Genotype:128 | Treatment Dosage:-	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__16_1986_A_F_128_0
SUBJECT_SAMPLE_FACTORS           	1987	34_1987_A_F_128_50	Sex:F | Genotype:128 | Treatment Dosage:50	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__34_1987_A_F_128_50
SUBJECT_SAMPLE_FACTORS           	1890	44_1890_A_F_128_0	Sex:F | Genotype:128 | Treatment Dosage:-	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__44_1890_A_F_128_0
SUBJECT_SAMPLE_FACTORS           	1956	106_1956_C_M_WT_0	Sex:M | Genotype:WT | Treatment Dosage:-	RAW_FILE_NAME=SC_20180814_HILICp_PRM_AP__106_1956_C_M_WT_0
#COLLECTION
CO:COLLECTION_SUMMARY            	For the single injection exposure, mice were sacrificed either 0, 1, 4, 12 or 24
CO:COLLECTION_SUMMARY            	hours after the injection to examine acute changes to Mn. For 1-week and 20-week
CO:COLLECTION_SUMMARY            	Mn exposures, mice were sacrificed 24 hours after the final injection. For all
CO:COLLECTION_SUMMARY            	studies, mice were sacrificed by cervical dislocation without anesthesia. Mice
CO:COLLECTION_SUMMARY            	were decapitated with sharp scissors and the brain removed. The striatum was
CO:COLLECTION_SUMMARY            	dissected out under a dissecting microscope, flash-frozen in liquid nitrogen and
CO:COLLECTION_SUMMARY            	stored at -80C until later analysis.
CO:SAMPLE_TYPE                   	Brain
#TREATMENT
TR:TREATMENT_SUMMARY             	This study utilized three Mn exposure paradigms: 1) a single subcutaneous
TR:TREATMENT_SUMMARY             	exposure of either a 1% solution of MnCl2•4(H2O) in filtered MilliQ water at
TR:TREATMENT_SUMMARY             	50 mg/kg body weight or vehicle (filtered water), 2) a 1-week, and 3) 20-week
TR:TREATMENT_SUMMARY             	chronic exposure. The 1-week exposure is a previously published week-long
TR:TREATMENT_SUMMARY             	exposure protocol (intermittent injections given on days 0, 3 and 6, with
TR:TREATMENT_SUMMARY             	sacrifice on day 7) known to elevate brain Mn levels1. The 20-week exposure is a
TR:TREATMENT_SUMMARY             	twice weekly injection (Mondays and Thursdays) based on the estimated half-life
TR:TREATMENT_SUMMARY             	of 3.5 days for Mn administered via this route and pilot work suggesting the
TR:TREATMENT_SUMMARY             	absence of observable toxicity with this exposure paradigm (including no weight
TR:TREATMENT_SUMMARY             	loss or physical signs of distress).
TR:TREATMENT_DOSE                	0, 5, 15, 50 mg/kg body weight
TR:TREATMENT_COMPOUND            	Mn
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	Previously frozen striatal tissue was lysed in 400µL ice-cold lysing buffer
SP:SAMPLEPREP_SUMMARY            	(1:1:2, ACN:MeOH:Ammonium Bicarbonate (0.1M, pH 8.0) (LC-MS grade). Individual
SP:SAMPLEPREP_SUMMARY            	samples were sonicated using a probe tip sonicator, 10 pulses, at 30% power and
SP:SAMPLEPREP_SUMMARY            	cooled down on ice between samples. A BCA protein assay was used to determine
SP:SAMPLEPREP_SUMMARY            	the protein concentration for each individual sample, and adjusted to a total
SP:SAMPLEPREP_SUMMARY            	amount of protein of 200µg in 200 µL of lysis buffer. Isotopically labeled
SP:SAMPLEPREP_SUMMARY            	standards, Creatinine-D3 and Lysine-D4, were added to each sample to assess
SP:SAMPLEPREP_SUMMARY            	sample processing steps (metabolite extraction and reconstitution). Following
SP:SAMPLEPREP_SUMMARY            	lysis and standard addition, protein precipitation was performed by adding
SP:SAMPLEPREP_SUMMARY            	800µL of ice-cold methanol (4x by volume). Samples were incubated at -80°C
SP:SAMPLEPREP_SUMMARY            	overnight. Following incubation, samples were centrifuged at 10,000 rpm for 10
SP:SAMPLEPREP_SUMMARY            	min to eliminate proteins. The supernatants containing metabolites were dried
SP:SAMPLEPREP_SUMMARY            	via speed-vacuum. Dried metabolite extracts were stored frozen at -80C until
SP:SAMPLEPREP_SUMMARY            	ready to use. Prior to mass spectrometry analysis, extracts were reconstituted
SP:SAMPLEPREP_SUMMARY            	in 100 μl of acetonitrile/ water (80:20, v/v) and centrifuged for 5 min at 15K
SP:SAMPLEPREP_SUMMARY            	rpm to remove insoluble material. Quality control samples were prepared by
SP:SAMPLEPREP_SUMMARY            	pooling equal volumes of each sample. Isotopically labeled standards, Valine-D8
SP:SAMPLEPREP_SUMMARY            	and Inosine-4N15, were added to each sample to determine MS instrument
SP:SAMPLEPREP_SUMMARY            	reproducibility.
SP:SAMPLEPREP_PROTOCOL_FILENAME  	Codreags00_20200327_075634_PR_MS_Metabolomics_Protocol.pdf
SP:PROCESSING_STORAGE_CONDITIONS 	-80℃
SP:EXTRACTION_METHOD             	Following lysis and standard addition, protein precipitation was performed by
SP:EXTRACTION_METHOD             	adding 800µL of ice-cold methanol (4x by volume). Samples were incubated at
SP:EXTRACTION_METHOD             	-80°C overnight. Following incubation, samples were centrifuged at 10,000 rpm
SP:EXTRACTION_METHOD             	for 10 min to eliminate proteins. The supernatants containing metabolites were
SP:EXTRACTION_METHOD             	dried via speed-vacuum.
SP:EXTRACT_STORAGE               	-80℃
SP:SAMPLE_RESUSPENSION           	100 μl of acetonitrile/ water (80:20, v/v) and centrifuged for 5 min at 15K rpm
SP:SAMPLE_RESUSPENSION           	to remove insoluble material
SP:SAMPLE_SPIKING                	Valine-D8 and Inosine-4N15, were added to each sample to determine MS instrument
SP:SAMPLE_SPIKING                	reproducibility.
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_TYPE           	HILIC
CH:INSTRUMENT_NAME               	Vanquish UHPLC binary system
CH:COLUMN_NAME                   	SeQuant ZIC-HILIC 3.5-µm, 2.1 mm × 100 mm column
CH:FLOW_RATE                     	0.2 mL/min
CH:COLUMN_TEMPERATURE            	40
CH:SOLVENT_A                     	5mM Ammonium formate in 90% water, 10% acetonitrile
CH:SOLVENT_B                     	5mM Ammonium formate in 90% acetonitrile, 10% water
#ANALYSIS
AN:ANALYSIS_TYPE                 	MS
AN:ANALYSIS_PROTOCOL_FILE        	Codreags00_20200327_075634_PR_MS_Metabolomics_Protocol.pdf
#MS
MS:INSTRUMENT_NAME               	Thermo Q Exactive HF hybrid Orbitrap
MS:INSTRUMENT_TYPE               	Orbitrap
MS:MS_TYPE                       	ESI
MS:ION_MODE                      	POSITIVE
MS:MS_COMMENTS                   	Ultra-performance liquid chromatography - tandem mass spectrometry (UPLC-MS/MS
MS:MS_COMMENTS                   	and UPLC-MS) raw data were imported, processed, normalized and reviewed using
MS:MS_COMMENTS                   	Progenesis QI v.2.1 (Non-linear Dynamics, Newcastle, UK).
MS:MS_RESULTS_FILE               	ST001333_AN002222_Results.txt	UNITS:peak area	Has m/z:Yes	Has RT:Yes	RT units:Minutes
#END