#METABOLOMICS WORKBENCH CLJenkins1219_20200713_134751 DATATRACK_ID:2095 STUDY_ID:ST001426 ANALYSIS_ID:AN002384 PROJECT_ID:PR000978 VERSION 1 CREATED_ON July 14, 2020, 12:10 pm #PROJECT PR:PROJECT_TITLE Influence of growth medium on the volatilomes of Pseudomonas spp. and PR:PROJECT_TITLE Staphylococcus spp. PR:PROJECT_TYPE Untargeted MS PR:PROJECT_SUMMARY Pseudomonas aeruginosa and Staphylococcus aureus are the predominant PR:PROJECT_SUMMARY opportunistic lung pathogens persons with CF [2017 CF Foundation Annual Report] PR:PROJECT_SUMMARY and are leading causes of respiratory failure and mortality [Malhotra, et al. PR:PROJECT_SUMMARY Clin Microbiol Rev, 2019]. Currently, sputum culture remains the standard of PR:PROJECT_SUMMARY care for lung infection detection, but sputum production is on the decline due PR:PROJECT_SUMMARY to improvements in CF therapies. To fill this diagnostic gap, we are working PR:PROJECT_SUMMARY toward the development of breath tests for lung infections by characterizing the PR:PROJECT_SUMMARY volatile metabolome (or “volatilome”) of P. aeruginosa and S. aureus. In PR:PROJECT_SUMMARY this study, we explored the influence of growth medium on the volatilomes of two PR:PROJECT_SUMMARY strains of P. aeruginosa (PAO1 and PA14) and S. aureus, as well as two other PR:PROJECT_SUMMARY species from the same genera, S. epidermidis and P. chlororaphis. We PR:PROJECT_SUMMARY hypothesized that the volatilomes would be influenced by the growth medium, but PR:PROJECT_SUMMARY that biological differences between these species and strains would dominate the PR:PROJECT_SUMMARY volatilomes and facilitate identification. PR:INSTITUTE Arizona State University PR:DEPARTMENT School of Life Sciences PR:LABORATORY Heather D. Bean Lab PR:LAST_NAME Bean, Ph.D. PR:FIRST_NAME Heather PR:ADDRESS PO Box 874501 Tempe, AZ 85287 PR:EMAIL heather.d.bean@asu.edu PR:PHONE 480-727-3395 PR:PUBLICATIONS Jenkins, C. L., H. D. Bean (2020). Influence of media on the differentiation of PR:PUBLICATIONS Staphylococcus spp. by volatile compounds. Journal of breath research 14, 016007 PR:PUBLICATIONS doi:10.1088/1752-7163/ab3e9d #STUDY ST:STUDY_TITLE Dependence of the Staphylococcal Volatilome Composition on Microbial Nutrition ST:STUDY_TYPE Untargeted MS ST:STUDY_SUMMARY Introduction: In vitro cultivation of staphylococci is fundamental to both ST:STUDY_SUMMARY clinical and research microbiology, and selection of growth medium will ST:STUDY_SUMMARY substantially influence staph growth rates, genetic integrity, pathogenicity, ST:STUDY_SUMMARY and metabolic capacity. Few studies, to-date, have investigated how the ST:STUDY_SUMMARY differences in rich media can influence the volatilome of cultivated bacteria. ST:STUDY_SUMMARY Objectives: The objective of this study was to determine the influence of rich ST:STUDY_SUMMARY media composition on the chemical characteristics of the volatilomes of ST:STUDY_SUMMARY Staphylococcus aureus and S. epidermidis. Methods: S. aureus (ATCC 12600) and S. ST:STUDY_SUMMARY epidermidis (ATCC 12228) were cultured in triplicate in four rich complex media ST:STUDY_SUMMARY (BHI, LB, MHB, and TSB), and the volatile metabolites produced by each culture ST:STUDY_SUMMARY were analyzed using headspace solid-phase microextraction combined with ST:STUDY_SUMMARY comprehensive two-dimensional gas chromatography – time-of-flight mass ST:STUDY_SUMMARY spectrometry (HS-SPME-GC×GC-TOFMS). Results: When comparing the chemical ST:STUDY_SUMMARY compositions of the staph volatilomes produced in each medium, we observed few ST:STUDY_SUMMARY differences when the presence versus absence of volatiles were compared. ST:STUDY_SUMMARY However, when the relative abundances of volatiles were included in the ST:STUDY_SUMMARY analyses, we observed that culturing staph in media containing free glucose (BHI ST:STUDY_SUMMARY and TSB) resulted in volatilomes dominated by acids and esters (67%). The ST:STUDY_SUMMARY low-glucose media (LB and MHB) produced ketones in greatest relative abundances, ST:STUDY_SUMMARY but the volatilome compositions in these two media were highly dissimilar. ST:STUDY_SUMMARY Conclusion: The staphylococcal volatilome is strongly influenced by the ST:STUDY_SUMMARY nutritional composition of the growth medium, especially the availability of ST:STUDY_SUMMARY free glucose, which is much more evident when the relative abundances of the ST:STUDY_SUMMARY volatiles are analyzed, compared to the presence versus absence. ST:INSTITUTE Arizona State University ST:DEPARTMENT School of Life Sciences ST:LABORATORY Heather D. Bean Lab ST:LAST_NAME Bean, Ph.D. ST:FIRST_NAME Heather ST:ADDRESS PO Box 874501 Tempe, AZ 85287 ST:EMAIL heather.d.bean@asu.edu ST:PHONE 4807273395 ST:STUDY_COMMENTS Staphylococcus aureus (ATCC 12600) and Stpahylococcus epidermidis (ATCC 12228) ST:STUDY_COMMENTS grown in four complex media ST:PUBLICATIONS Jenkins, C. L., H. D. Bean (2020). Influence of media on the differentiation of ST:PUBLICATIONS Staphylococcus spp. by volatile compounds. Journal of breath research 14, 016007 ST:PUBLICATIONS doi:10.1088/1752-7163/ab3e9d #SUBJECT SU:SUBJECT_TYPE Bacteria SU:SUBJECT_SPECIES Staphylococcus epidermidis SU:TAXONOMY_ID 1282 SU:GENOTYPE_STRAIN ATCC 12600; ATCC 12228 SU:CELL_BIOSOURCE_OR_SUPPLIER ATCC SU:CELL_STRAIN_DETAILS ATCC 12600; ATCC 12228 #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data SUBJECT_SAMPLE_FACTORS Media MediaBlank1_BHI_1 Treatment:BLANK | Culture Medium:BHI Culture Time=24; RAW_FILE_NAME=MediaBlank1_BHI_1.SMP SUBJECT_SAMPLE_FACTORS Media MediaBlank1_BHI_2 Treatment:BLANK | Culture Medium:BHI Culture Time=24; RAW_FILE_NAME=MediaBlank1_BHI_2.SMP SUBJECT_SAMPLE_FACTORS Media MediaBlank1_BHI_3 Treatment:BLANK | Culture Medium:BHI Culture Time=24; RAW_FILE_NAME=MediaBlank1_BHI_3.SMP SUBJECT_SAMPLE_FACTORS Media MediaBlank1_LB_1 Treatment:BLANK | Culture Medium:LB_LENNOX Culture Time=24; RAW_FILE_NAME=MediaBlank1_LB_1.SMP SUBJECT_SAMPLE_FACTORS Media MediaBlank1_LB_2 Treatment:BLANK | Culture Medium:LB_LENNOX Culture Time=24; RAW_FILE_NAME=MediaBlank1_LB_2.SMP SUBJECT_SAMPLE_FACTORS Media MediaBlank1_LB_3 Treatment:BLANK | Culture Medium:LB_LENNOX Culture Time=24; RAW_FILE_NAME=MediaBlank1_LB_3.SMP SUBJECT_SAMPLE_FACTORS Media MediaBlank1_MH_1 Treatment:BLANK | Culture Medium:MUELLER-HINTON Culture Time=24; RAW_FILE_NAME=MediaBlank1_MH_1.SMP SUBJECT_SAMPLE_FACTORS Media MediaBlank1_MH_2 Treatment:BLANK | Culture Medium:MUELLER-HINTON Culture Time=24; RAW_FILE_NAME=MediaBlank1_MH_2.SMP SUBJECT_SAMPLE_FACTORS Media MediaBlank1_MH_3 Treatment:BLANK | Culture Medium:MUELLER-HINTON Culture Time=24; RAW_FILE_NAME=MediaBlank1_MH_3.SMP SUBJECT_SAMPLE_FACTORS Media MediaBlank1_TSB_1 Treatment:BLANK | Culture Medium:TSB Culture Time=24; RAW_FILE_NAME=MediaBlank1_TSB_1.SMP SUBJECT_SAMPLE_FACTORS Media MediaBlank1_TSB_2 Treatment:BLANK | Culture Medium:TSB Culture Time=24; RAW_FILE_NAME=MediaBlank1_TSB_2.SMP SUBJECT_SAMPLE_FACTORS Media MediaBlank1_TSB_3 Treatment:BLANK | Culture Medium:TSB Culture Time=24; RAW_FILE_NAME=MediaBlank1_TSB_3.SMP SUBJECT_SAMPLE_FACTORS Media MediaBlank2_BHI_1 Treatment:BLANK | Culture Medium:BHI Culture Time=24; RAW_FILE_NAME=MediaBlank2_BHI_1.SMP SUBJECT_SAMPLE_FACTORS Media MediaBlank2_BHI_2 Treatment:BLANK | Culture Medium:BHI Culture Time=24; RAW_FILE_NAME=MediaBlank2_BHI_2.SMP SUBJECT_SAMPLE_FACTORS Media MediaBlank2_BHI_3 Treatment:BLANK | Culture Medium:BHI Culture Time=24; RAW_FILE_NAME=MediaBlank2_BHI_3.SMP SUBJECT_SAMPLE_FACTORS Media MediaBlank2_LB_1 Treatment:BLANK | Culture Medium:LB_LENNOX Culture Time=24; RAW_FILE_NAME=MediaBlank2_LB_1.SMP SUBJECT_SAMPLE_FACTORS Media MediaBlank2_LB_2 Treatment:BLANK | Culture Medium:LB_LENNOX Culture Time=24; RAW_FILE_NAME=MediaBlank2_LB_2.SMP SUBJECT_SAMPLE_FACTORS Media MediaBlank2_LB_3 Treatment:BLANK | Culture Medium:LB_LENNOX Culture Time=24; RAW_FILE_NAME=MediaBlank2_LB_3.SMP SUBJECT_SAMPLE_FACTORS Media MediaBlank2_MH_1 Treatment:BLANK | Culture Medium:MUELLER-HINTON Culture Time=24; RAW_FILE_NAME=MediaBlank2_MH_1.SMP SUBJECT_SAMPLE_FACTORS Media MediaBlank2_MH_2 Treatment:BLANK | Culture Medium:MUELLER-HINTON Culture Time=24; RAW_FILE_NAME=MediaBlank2_MH_2.SMP SUBJECT_SAMPLE_FACTORS Media MediaBlank2_MH_3 Treatment:BLANK | Culture Medium:MUELLER-HINTON Culture Time=24; RAW_FILE_NAME=MediaBlank2_MH_3.SMP SUBJECT_SAMPLE_FACTORS PA01 PA01_BHI_1 Treatment:MEDIUM | Culture Medium:BHI Culture Time=24; RAW_FILE_NAME=PA01_BHI_1.SMP SUBJECT_SAMPLE_FACTORS PA01 PA01_BHI_2 Treatment:MEDIUM | Culture Medium:BHI Culture Time=24; RAW_FILE_NAME=PA01_BHI_2.SMP SUBJECT_SAMPLE_FACTORS PA01 PA01_BHI_3 Treatment:MEDIUM | Culture Medium:BHI Culture Time=24; RAW_FILE_NAME=PA01_BHI_3.SMP SUBJECT_SAMPLE_FACTORS PA01 PA01_LB_1 Treatment:MEDIUM | Culture Medium:LB_LENNOX Culture Time=24; RAW_FILE_NAME=PA01_LB_1.SMP SUBJECT_SAMPLE_FACTORS PA01 PA01_LB_2 Treatment:MEDIUM | Culture Medium:LB_LENNOX Culture Time=24; RAW_FILE_NAME=PA01_LB_2.SMP SUBJECT_SAMPLE_FACTORS PA01 PA01_LB_3 Treatment:MEDIUM | Culture Medium:LB_LENNOX Culture Time=24; RAW_FILE_NAME=PA01_LB_3.SMP SUBJECT_SAMPLE_FACTORS PA01 PA01_MH_1 Treatment:MEDIUM | Culture Medium:MUELLER-HINTON Culture Time=24; RAW_FILE_NAME=PA01_MH_1.SMP SUBJECT_SAMPLE_FACTORS PA01 PA01_MH_2 Treatment:MEDIUM | Culture Medium:MUELLER-HINTON Culture Time=24; RAW_FILE_NAME=PA01_MH_2.SMP SUBJECT_SAMPLE_FACTORS PA01 PA01_MH_3 Treatment:MEDIUM | Culture Medium:MUELLER-HINTON Culture Time=24; RAW_FILE_NAME=PA01_MH_3.SMP SUBJECT_SAMPLE_FACTORS PA14 PA14_BHI_1 Treatment:MEDIUM | Culture Medium:BHI Culture Time=24; RAW_FILE_NAME=PA14_BHI_1.SMP SUBJECT_SAMPLE_FACTORS PA14 PA14_BHI_2 Treatment:MEDIUM | Culture Medium:BHI Culture Time=24; RAW_FILE_NAME=PA14_BHI_2.SMP SUBJECT_SAMPLE_FACTORS PA14 PA14_BHI_3 Treatment:MEDIUM | Culture Medium:BHI Culture Time=24; RAW_FILE_NAME=PA14_BHI_3.SMP SUBJECT_SAMPLE_FACTORS PA14 PA14_LB_1 Treatment:MEDIUM | Culture Medium:LB_LENNOX Culture Time=24; RAW_FILE_NAME=PA14_LB_1.SMP SUBJECT_SAMPLE_FACTORS PA14 PA14_LB_2 Treatment:MEDIUM | Culture Medium:LB_LENNOX Culture Time=24; RAW_FILE_NAME=PA14_LB_2.SMP SUBJECT_SAMPLE_FACTORS PA14 PA14_LB_3 Treatment:MEDIUM | Culture Medium:LB_LENNOX Culture Time=24; RAW_FILE_NAME=PA14_LB_3.SMP SUBJECT_SAMPLE_FACTORS PA14 PA14_MH_1 Treatment:MEDIUM | Culture Medium:MUELLER-HINTON Culture Time=24; RAW_FILE_NAME=PA14_MH_1.SMP SUBJECT_SAMPLE_FACTORS PA14 PA14_MH_2 Treatment:MEDIUM | Culture Medium:MUELLER-HINTON Culture Time=24; RAW_FILE_NAME=PA14_MH_2.SMP SUBJECT_SAMPLE_FACTORS PA14 PA14_MH_3 Treatment:MEDIUM | Culture Medium:MUELLER-HINTON Culture Time=24; RAW_FILE_NAME=PA14_MH_3.SMP SUBJECT_SAMPLE_FACTORS Pcl Pcl_BHI_1 Treatment:MEDIUM | Culture Medium:BHI Culture Time=24; RAW_FILE_NAME=Pcl_BHI_1.SMP SUBJECT_SAMPLE_FACTORS Pcl Pcl_BHI_2 Treatment:MEDIUM | Culture Medium:BHI Culture Time=24; RAW_FILE_NAME=Pcl_BHI_2.SMP SUBJECT_SAMPLE_FACTORS Pcl Pcl_BHI_3 Treatment:MEDIUM | Culture Medium:BHI Culture Time=24; RAW_FILE_NAME=Pcl_BHI_3.SMP SUBJECT_SAMPLE_FACTORS Pcl Pcl_LB_1 Treatment:MEDIUM | Culture Medium:LB_LENNOX Culture Time=24; RAW_FILE_NAME=Pcl_LB_1.SMP SUBJECT_SAMPLE_FACTORS Pcl Pcl_LB_2 Treatment:MEDIUM | Culture Medium:LB_LENNOX Culture Time=24; RAW_FILE_NAME=Pcl_LB_2.SMP SUBJECT_SAMPLE_FACTORS Pcl Pcl_LB_3 Treatment:MEDIUM | Culture Medium:LB_LENNOX Culture Time=24; RAW_FILE_NAME=Pcl_LB_3.SMP SUBJECT_SAMPLE_FACTORS Pcl Pcl_MH_1 Treatment:MEDIUM | Culture Medium:MUELLER-HINTON Culture Time=24; RAW_FILE_NAME=Pcl_MH_1.SMP SUBJECT_SAMPLE_FACTORS Pcl Pcl_MH_2 Treatment:MEDIUM | Culture Medium:MUELLER-HINTON Culture Time=24; RAW_FILE_NAME=Pcl_MH_2.SMP SUBJECT_SAMPLE_FACTORS Pcl Pcl_MH_3 Treatment:MEDIUM | Culture Medium:MUELLER-HINTON Culture Time=24; RAW_FILE_NAME=Pcl_MH_3.SMP SUBJECT_SAMPLE_FACTORS Sa Staphau_BHI_1 Treatment:MEDIUM | Culture Medium:BHI Culture Time=24; RAW_FILE_NAME=Staphau_BHI_1.SMP SUBJECT_SAMPLE_FACTORS Sa Staphau_BHI_2 Treatment:MEDIUM | Culture Medium:BHI Culture Time=24; RAW_FILE_NAME=Staphau_BHI_2.SMP SUBJECT_SAMPLE_FACTORS Sa Staphau_BHI_3 Treatment:MEDIUM | Culture Medium:BHI Culture Time=24; RAW_FILE_NAME=Staphau_BHI_3.SMP SUBJECT_SAMPLE_FACTORS Sa Staphau_LB_1 Treatment:MEDIUM | Culture Medium:LB_LENNOX Culture Time=24; RAW_FILE_NAME=Staphau_LB_1.SMP SUBJECT_SAMPLE_FACTORS Sa Staphau_LB_2 Treatment:MEDIUM | Culture Medium:LB_LENNOX Culture Time=24; RAW_FILE_NAME=Staphau_LB_2.SMP SUBJECT_SAMPLE_FACTORS Sa Staphau_LB_3 Treatment:MEDIUM | Culture Medium:LB_LENNOX Culture Time=24; RAW_FILE_NAME=Staphau_LB_3.SMP SUBJECT_SAMPLE_FACTORS Sa Staphau_MH_1 Treatment:MEDIUM | Culture Medium:MUELLER-HINTON Culture Time=24; RAW_FILE_NAME=Staphau_MH_1.SMP SUBJECT_SAMPLE_FACTORS Sa Staphau_MH_2 Treatment:MEDIUM | Culture Medium:MUELLER-HINTON Culture Time=24; RAW_FILE_NAME=Staphau_MH_2.SMP SUBJECT_SAMPLE_FACTORS Sa Staphau_MH_3 Treatment:MEDIUM | Culture Medium:MUELLER-HINTON Culture Time=24; RAW_FILE_NAME=Staphau_MH_3.SMP SUBJECT_SAMPLE_FACTORS Sa Staphau_TSB_1 Treatment:MEDIUM | Culture Medium:TSB Culture Time=24; RAW_FILE_NAME=Staphau TSB 1.SMP SUBJECT_SAMPLE_FACTORS Sa Staphau_TSB_2 Treatment:MEDIUM | Culture Medium:TSB Culture Time=24; RAW_FILE_NAME=Staphau_TSB_2.SMP SUBJECT_SAMPLE_FACTORS Sa Staphau_TSB_3 Treatment:MEDIUM | Culture Medium:TSB Culture Time=24; RAW_FILE_NAME=Staphau TSB 3.SMP SUBJECT_SAMPLE_FACTORS Se Staphep_BHI_1 Treatment:MEDIUM | Culture Medium:BHI Culture Time=24; RAW_FILE_NAME=Staphep_BHI_1.SMP SUBJECT_SAMPLE_FACTORS Se Staphep_BHI_2 Treatment:MEDIUM | Culture Medium:BHI Culture Time=24; RAW_FILE_NAME=Staphep_BHI_2.SMP SUBJECT_SAMPLE_FACTORS Se Staphep_BHI_3 Treatment:MEDIUM | Culture Medium:BHI Culture Time=24; RAW_FILE_NAME=Staphep_BHI_3.SMP SUBJECT_SAMPLE_FACTORS Se Staphep_LB_1 Treatment:MEDIUM | Culture Medium:LB_LENNOX Culture Time=24; RAW_FILE_NAME=Staphep_LB_1.SMP SUBJECT_SAMPLE_FACTORS Se Staphep_LB_2 Treatment:MEDIUM | Culture Medium:LB_LENNOX Culture Time=24; RAW_FILE_NAME=Staphep_LB_2.SMP SUBJECT_SAMPLE_FACTORS Se Staphep_LB_3 Treatment:MEDIUM | Culture Medium:LB_LENNOX Culture Time=24; RAW_FILE_NAME=Staphep_LB_3.SMP SUBJECT_SAMPLE_FACTORS Se Staphep_MH_1 Treatment:MEDIUM | Culture Medium:MUELLER-HINTON Culture Time=24; RAW_FILE_NAME=Staphep_MH_1.SMP SUBJECT_SAMPLE_FACTORS Se Staphep_MH_2 Treatment:MEDIUM | Culture Medium:MUELLER-HINTON Culture Time=24; RAW_FILE_NAME=Staphep_MH_2.SMP SUBJECT_SAMPLE_FACTORS Se Staphep_MH_3 Treatment:MEDIUM | Culture Medium:MUELLER-HINTON Culture Time=24; RAW_FILE_NAME=Staphep_MH_3.SMP SUBJECT_SAMPLE_FACTORS Se Staphep_TSB_1 Treatment:MEDIUM | Culture Medium:TSB Culture Time=24; RAW_FILE_NAME=Staphep_TSB_1.SMP SUBJECT_SAMPLE_FACTORS Se Staphep_TSB_2 Treatment:MEDIUM | Culture Medium:TSB Culture Time=24; RAW_FILE_NAME=Staphep_TSB_2.SMP SUBJECT_SAMPLE_FACTORS Se Staphep_TSB_3 Treatment:MEDIUM | Culture Medium:TSB Culture Time=24; RAW_FILE_NAME=Staphep_TSB_3.SMP SUBJECT_SAMPLE_FACTORS Alkane mixture KIMix_1 Treatment:Chemical Standards | Culture Medium:none Culture Time=-; RAW_FILE_NAME=KIMix_1_.SMP SUBJECT_SAMPLE_FACTORS Alkane mixture KIMix_2 Treatment:Chemical Standards | Culture Medium:none Culture Time=-; RAW_FILE_NAME=KIMix_2_.SMP SUBJECT_SAMPLE_FACTORS Alkane mixture KIMix_3 Treatment:Chemical Standards | Culture Medium:none Culture Time=-; RAW_FILE_NAME=KIMix_3_.SMP SUBJECT_SAMPLE_FACTORS Empty sampling vial Vial-Blank_1 Treatment:Vial Blank | Culture Medium:none Culture Time=-; RAW_FILE_NAME=Vial-Blank_1.SMP SUBJECT_SAMPLE_FACTORS Empty sampling vial VIAL_BLANK_1 Treatment:Vial Blank | Culture Medium:none Culture Time=-; RAW_FILE_NAME=VIAL_BLANK_1 #COLLECTION CO:COLLECTION_SUMMARY Staphylococcus aureus (ATCC 12600) and Staphylococcus epidermidis (ATCC 12228) CO:COLLECTION_SUMMARY were cultured in four filter-sterilized complex media for metabolomics analysis: CO:COLLECTION_SUMMARY Brain Heart Infusion broth (BHI; Bacto); lysogeny broth Lennox (LB; Fisher CO:COLLECTION_SUMMARY Scientific); Mueller Hinton broth (MHB; Difco); and Tryptic Soy broth (TSB; CO:COLLECTION_SUMMARY Bacto). Each species was prepared in biological triplicate. Uninoculated media CO:COLLECTION_SUMMARY control blanks were prepared in six replicates for BHI, LB, and MHB, and in CO:COLLECTION_SUMMARY triplicate for TSB, following the same procedures as the bacterial samples and CO:COLLECTION_SUMMARY processed in parallel. Samples were transferred to capped GC vials and stored at CO:COLLECTION_SUMMARY -20 °C for approximately two weeks prior to GC×GC-TOFMS analysis. CO:COLLECTION_PROTOCOL_FILENAME CLJenkins1210_2020713_134751_Collection_Protocol_Metadata.txt CO:SAMPLE_TYPE Bacterial cells CO:COLLECTION_FREQUENCY Once, at the completion of 24 hour aerobic incubation at 37 C with orbital CO:COLLECTION_FREQUENCY shaking #TREATMENT TR:TREATMENT_SUMMARY Two ATCC strains of staphylococci were grown in four different filter-sterilized TR:TREATMENT_SUMMARY complex media under identical conditions. Please see the Collection Protocol for TR:TREATMENT_SUMMARY details. TR:CELL_MEDIA BHI Broth, LB Lennox, Mueller Hinton Broth, Tryptic Soy Broth #SAMPLEPREP SP:SAMPLEPREP_SUMMARY No sample preparation was required due to sampling via Head-Space Solid-phase SP:SAMPLEPREP_SUMMARY microextraction by autosampler robot. #CHROMATOGRAPHY CH:CHROMATOGRAPHY_SUMMARY Comprehensive two-dimensional gas chromatography with multi-dimensional column CH:CHROMATOGRAPHY_SUMMARY configuration CH:CHROMATOGRAPHY_TYPE GCxGC CH:INSTRUMENT_NAME Agilent 7890B CH:COLUMN_NAME Multidimensional column configuration #ANALYSIS AN:ANALYSIS_TYPE MS #MS MS:INSTRUMENT_NAME Leco Pegasus 4D GCxGC TOF MS:INSTRUMENT_TYPE GC x GC-TOF MS:MS_TYPE EI MS:ION_MODE POSITIVE MS:MS_COMMENTS none MS:MS_RESULTS_FILE ST001426_AN002384_Results.txt UNITS:peak area Has m/z:Yes Has RT:Yes RT units:Seconds #END