#METABOLOMICS WORKBENCH depnerc_20190829_115830 DATATRACK_ID:1808 STUDY_ID:ST001440 ANALYSIS_ID:AN002405 PROJECT_ID:PR000990
VERSION             	1
CREATED_ON             	August 4, 2020, 3:30 pm
#PROJECT
PR:PROJECT_TITLE                 	Developing preliminary blood metabolomics-based biomarkers of insufficient sleep
PR:PROJECT_TITLE                 	in humans
PR:PROJECT_TYPE                  	Human clinical translational study
PR:PROJECT_SUMMARY               	Study Objective: Identify small molecule biomarkers of insufficient sleep using
PR:PROJECT_SUMMARY               	untargeted plasma metabolomics in humans undergoing experimental insufficient
PR:PROJECT_SUMMARY               	sleep. Methods: We conducted a cross-over laboratory study where 16 normal
PR:PROJECT_SUMMARY               	weight participants (8 men; age 22 ± 5 years; body mass index < 25 kg/m2)
PR:PROJECT_SUMMARY               	completed three baseline days (BL; 9h sleep opportunity per night) followed by
PR:PROJECT_SUMMARY               	five day insufficient (5H; 5h sleep opportunity per night) and adequate (9H; 9h
PR:PROJECT_SUMMARY               	sleep opportunity per night) sleep conditions. Energy balanced diets were
PR:PROJECT_SUMMARY               	provided during baseline, with ad libitum energy intake provided during the
PR:PROJECT_SUMMARY               	insufficient and adequate sleep conditions. Untargeted plasma metabolomics
PR:PROJECT_SUMMARY               	analyses were performed using blood samples collected every 4h across the final
PR:PROJECT_SUMMARY               	24h of each condition. Biomarker models were developed using logistic regression
PR:PROJECT_SUMMARY               	and linear support vector machine algorithms. Results: The top performing
PR:PROJECT_SUMMARY               	biomarker model was developed by linear support vector machine modeling,
PR:PROJECT_SUMMARY               	consisted of 65 compounds, and discriminated insufficient versus adequate sleep
PR:PROJECT_SUMMARY               	with 74% overall accuracy and a Matthew’s Correlation Coefficient of 0.39. The
PR:PROJECT_SUMMARY               	compounds in the top performing biomarker model were associated with ATP Binding
PR:PROJECT_SUMMARY               	Cassette Transporters in Lipid Homeostasis, Phospholipid Metabolic Process,
PR:PROJECT_SUMMARY               	Plasma Lipoprotein Remodeling, and sphingolipid metabolism. Conclusion: We
PR:PROJECT_SUMMARY               	identified potential metabolomics-based biomarkers of insufficient sleep in
PR:PROJECT_SUMMARY               	humans. Further development and validation of omics-based biomarkers of
PR:PROJECT_SUMMARY               	insufficient sleep will advance our understanding of the negative consequences
PR:PROJECT_SUMMARY               	of insufficient sleep, improve diagnosis of poor sleep health, and identify
PR:PROJECT_SUMMARY               	targets for countermeasures designed to mitigate the negative health
PR:PROJECT_SUMMARY               	consequences of insufficient sleep.
PR:INSTITUTE                     	University of Colorado Boulder
PR:DEPARTMENT                    	Integrative Physiology
PR:LABORATORY                    	Sleep and Chronobiology Laboratory, University of Colorado Boulder
PR:LAST_NAME                     	Depner
PR:FIRST_NAME                    	Christopher
PR:ADDRESS                       	354 UCB
PR:EMAIL                         	christopher.depner@colorado.edu
PR:PHONE                         	5098799151
PR:FUNDING_SOURCE                	NIH; Sleep Research Society Foundation
#STUDY
ST:STUDY_TITLE                   	Developing preliminary blood metabolomics-based biomarkers of insufficient sleep
ST:STUDY_TITLE                   	in humans
ST:STUDY_SUMMARY                 	Study Objective: Identify small molecule biomarkers of insufficient sleep using
ST:STUDY_SUMMARY                 	untargeted plasma metabolomics in humans undergoing experimental insufficient
ST:STUDY_SUMMARY                 	sleep. Methods: We conducted a cross-over laboratory study where 16 normal
ST:STUDY_SUMMARY                 	weight participants (8 men; age 22 ± 5 years; body mass index < 25 kg/m2)
ST:STUDY_SUMMARY                 	completed three baseline days (BL; 9h sleep opportunity per night) followed by
ST:STUDY_SUMMARY                 	five day insufficient (5H; 5h sleep opportunity per night) and adequate (9H; 9h
ST:STUDY_SUMMARY                 	sleep opportunity per night) sleep conditions. Energy balanced diets were
ST:STUDY_SUMMARY                 	provided during baseline, with ad libitum energy intake provided during the
ST:STUDY_SUMMARY                 	insufficient and adequate sleep conditions. Untargeted plasma metabolomics
ST:STUDY_SUMMARY                 	analyses were performed using blood samples collected every 4h across the final
ST:STUDY_SUMMARY                 	24h of each condition. Biomarker models were developed using logistic regression
ST:STUDY_SUMMARY                 	and linear support vector machine algorithms. Results: The top performing
ST:STUDY_SUMMARY                 	biomarker model was developed by linear support vector machine modeling,
ST:STUDY_SUMMARY                 	consisted of 65 compounds, and discriminated insufficient versus adequate sleep
ST:STUDY_SUMMARY                 	with 74% overall accuracy and a Matthew’s Correlation Coefficient of 0.39. The
ST:STUDY_SUMMARY                 	compounds in the top performing biomarker model were associated with ATP Binding
ST:STUDY_SUMMARY                 	Cassette Transporters in Lipid Homeostasis, Phospholipid Metabolic Process,
ST:STUDY_SUMMARY                 	Plasma Lipoprotein Remodeling, and sphingolipid metabolism. Conclusion: We
ST:STUDY_SUMMARY                 	identified potential metabolomics-based biomarkers of insufficient sleep in
ST:STUDY_SUMMARY                 	humans. Further development and validation of omics-based biomarkers of
ST:STUDY_SUMMARY                 	insufficient sleep will advance our understanding of the negative consequences
ST:STUDY_SUMMARY                 	of insufficient sleep, improve diagnosis of poor sleep health, and identify
ST:STUDY_SUMMARY                 	targets for countermeasures designed to mitigate the negative health
ST:STUDY_SUMMARY                 	consequences of insufficient sleep.
ST:INSTITUTE                     	University of Colorado Boulder
ST:LAST_NAME                     	Depner
ST:FIRST_NAME                    	Christopher
ST:ADDRESS                       	354 UCB, Clare Small Room 114
ST:EMAIL                         	christopher.depner@colorado.edu
ST:PHONE                         	5098799151
#SUBJECT
SU:SUBJECT_TYPE                  	Human
SU:SUBJECT_SPECIES               	Homo sapiens
SU:TAXONOMY_ID                   	9606
SU:GENDER                        	Male and female
#FACTORS
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data
SUBJECT_SAMPLE_FACTORS           	Subject_1	1_5H_0	Gender:M | Time:zero | Sleep Window (Hours):5H	RAW_FILE_NAME=Aqueous_B6_01_5H_T0A.d Lipid_Pos_B6_01_5H_T0.d
SUBJECT_SAMPLE_FACTORS           	Subject_1	1_5H_4	Gender:M | Time:4 | Sleep Window (Hours):5H	RAW_FILE_NAME=Aqueous_B3_01_5H_T4_RRB4.d Lipid_Pos_B4_01_5H_T4.d
SUBJECT_SAMPLE_FACTORS           	Subject_1	1_9H_12	Gender:M | Time:12 | Sleep Window (Hours):9H	RAW_FILE_NAME=Aqueous_B3_01_9H_T12.d Lipid_Pos_B2_01_9H_T12.d
SUBJECT_SAMPLE_FACTORS           	Subject_1	1_9H_16	Gender:M | Time:16 | Sleep Window (Hours):9H	RAW_FILE_NAME=Aqueous_B3_01_9H_T16.d Lipid_Pos_B6_01_9H_T16.d
SUBJECT_SAMPLE_FACTORS           	Subject_1	1_9H_20	Gender:M | Time:20 | Sleep Window (Hours):9H	RAW_FILE_NAME=Aqueous_B3_01_9H_T20.d Lipid_Pos_B3_01_9H_T20.d
SUBJECT_SAMPLE_FACTORS           	Subject_1	1_9H_4	Gender:M | Time:4 | Sleep Window (Hours):9H	RAW_FILE_NAME=Aqueous_B4_01_9H_T4.d Lipid_Pos_B1_01_9H_T4.d
SUBJECT_SAMPLE_FACTORS           	Subject_1	1_9H_8	Gender:M | Time:8 | Sleep Window (Hours):9H	RAW_FILE_NAME=Aqueous_B4_01_9H_T8.d Lipid_Pos_B5_01_9H_T8.d
SUBJECT_SAMPLE_FACTORS           	Subject_1	1_BL_0	Gender:M | Time:zero | Sleep Window (Hours):BL	RAW_FILE_NAME=Aqueous_B2_01_BL_T0.d Lipid_Pos_B1_01_BL_T0.d
SUBJECT_SAMPLE_FACTORS           	Subject_1	1_BL_12	Gender:M | Time:12 | Sleep Window (Hours):BL	RAW_FILE_NAME=Aqueous_B6_01_BL_T12.d Lipid_Pos_B3_01_BL_T12_B.d
SUBJECT_SAMPLE_FACTORS           	Subject_1	1_BL_16	Gender:M | Time:16 | Sleep Window (Hours):BL	RAW_FILE_NAME=Aqueous_B5_01_BL_T16.d Lipid_Pos_B4_01_BL_T16.d
SUBJECT_SAMPLE_FACTORS           	Subject_1	1_BL_20	Gender:M | Time:20 | Sleep Window (Hours):BL	RAW_FILE_NAME=Aqueous_B3_01_BL_T20.d Lipid_Pos_B2_01_BL_T20.d
SUBJECT_SAMPLE_FACTORS           	Subject_1	1_BL_4	Gender:M | Time:4 | Sleep Window (Hours):BL	RAW_FILE_NAME=Aqueous_B9_01_BL_T4.d Lipid_Pos_B5_01_BL_T4.d
SUBJECT_SAMPLE_FACTORS           	Subject_1	1_BL_8	Gender:M | Time:8 | Sleep Window (Hours):BL	RAW_FILE_NAME=Aqueous_B2_01_BL_T8.d Lipid_Pos_B6_01_BL_T8_B.d
SUBJECT_SAMPLE_FACTORS           	Subject_11	11_5H_12	Gender:M | Time:12 | Sleep Window (Hours):5H	RAW_FILE_NAME=Aqueous_B7_11_5H_T12.d Lipid_Pos_B2_11_5H_T12.d
SUBJECT_SAMPLE_FACTORS           	Subject_11	11_5H_16	Gender:M | Time:16 | Sleep Window (Hours):5H	RAW_FILE_NAME=Aqueous_B2_11_5H_T16.d Lipid_Pos_B7_11_5H_T16.d
SUBJECT_SAMPLE_FACTORS           	Subject_11	11_5H_20	Gender:M | Time:20 | Sleep Window (Hours):5H	RAW_FILE_NAME=Aqueous_B8_11_5H_T20.d Lipid_Pos_B4_11_5H_T20.d
SUBJECT_SAMPLE_FACTORS           	Subject_11	11_5H_4	Gender:M | Time:4 | Sleep Window (Hours):5H	RAW_FILE_NAME=Aqueous_B8_11_5H_T4.d Lipid_Pos_B4_11_5H_T4.d
SUBJECT_SAMPLE_FACTORS           	Subject_11	11_5H_8	Gender:M | Time:8 | Sleep Window (Hours):5H	RAW_FILE_NAME=Aqueous_B4_11_5H_T8.d Lipid_Pos_B4_11_5H_T8.d
SUBJECT_SAMPLE_FACTORS           	Subject_11	11_9H_0	Gender:M | Time:zero | Sleep Window (Hours):9H	RAW_FILE_NAME=Aqueous_B3_11_9H_T0.d Lipid_Pos_B6_11_9H_T0.d
SUBJECT_SAMPLE_FACTORS           	Subject_11	11_9H_12	Gender:M | Time:12 | Sleep Window (Hours):9H	RAW_FILE_NAME=Aqueous_B6_11_9H_T12.d Lipid_Pos_B3_11_9H_T12.d
SUBJECT_SAMPLE_FACTORS           	Subject_11	11_9H_16	Gender:M | Time:16 | Sleep Window (Hours):9H	RAW_FILE_NAME=Aqueous_B6_11_9H_T16.d Lipid_Pos_B1_11_9H_T16.d
SUBJECT_SAMPLE_FACTORS           	Subject_11	11_9H_4	Gender:M | Time:4 | Sleep Window (Hours):9H	RAW_FILE_NAME=Aqueous_B9_11_9H_T4.d Lipid_Pos_B5_11_9H_T4.d
SUBJECT_SAMPLE_FACTORS           	Subject_11	11_9H_8	Gender:M | Time:8 | Sleep Window (Hours):9H	RAW_FILE_NAME=Aqueous_B9_11_9H_T8.d Lipid_Pos_B1_11_9H_T8.d
SUBJECT_SAMPLE_FACTORS           	Subject_11	11_BL_0	Gender:M | Time:zero | Sleep Window (Hours):BL	RAW_FILE_NAME=Aqueous_B4_11_BL_T0.d Lipid_Pos_B4_11_BL_T0.d
SUBJECT_SAMPLE_FACTORS           	Subject_11	11_BL_12	Gender:M | Time:12 | Sleep Window (Hours):BL	RAW_FILE_NAME=Aqueous_B2_11_BL_T12.d Lipid_Pos_B6_11_BL_T12.d
SUBJECT_SAMPLE_FACTORS           	Subject_11	11_BL_16	Gender:M | Time:16 | Sleep Window (Hours):BL	RAW_FILE_NAME=Aqueous_B3_11_BL_T16A.d Lipid_Pos_B5_11_BL_T16.d
SUBJECT_SAMPLE_FACTORS           	Subject_11	11_BL_20	Gender:M | Time:20 | Sleep Window (Hours):BL	RAW_FILE_NAME=Aqueous_B5_11_BL_T20.d Lipid_Pos_B3_11_BL_T20.d
SUBJECT_SAMPLE_FACTORS           	Subject_11	11_BL_4	Gender:M | Time:4 | Sleep Window (Hours):BL	RAW_FILE_NAME=Aqueous_B3_11_BL_T4.d Lipid_Pos_B1_11_BL_T4.d
SUBJECT_SAMPLE_FACTORS           	Subject_11	11_BL_8	Gender:M | Time:8 | Sleep Window (Hours):BL	RAW_FILE_NAME=Aqueous_B5_11_BL_T8.d Lipid_Pos_B1_11_BL_T8.d
SUBJECT_SAMPLE_FACTORS           	Subject_14	14_5H_0	Gender:M | Time:zero | Sleep Window (Hours):5H	RAW_FILE_NAME=Aqueous_B7_14_5H_T0.d Lipid_Pos_B1_14_5H_T0_B.d
SUBJECT_SAMPLE_FACTORS           	Subject_14	14_5H_16	Gender:M | Time:16 | Sleep Window (Hours):5H	RAW_FILE_NAME=Aqueous_B6_14_5H_T16.d Lipid_Pos_B4_14_5H_T16_Rerun.d
SUBJECT_SAMPLE_FACTORS           	Subject_14	14_5H_20	Gender:M | Time:20 | Sleep Window (Hours):5H	RAW_FILE_NAME=Aqueous_B5_14_5H_T20.d Lipid_Pos_B7_14_5H_T20.d
SUBJECT_SAMPLE_FACTORS           	Subject_14	14_5H_4	Gender:M | Time:4 | Sleep Window (Hours):5H	RAW_FILE_NAME=Aqueous_B8_14_5H_T4_RS.d Lipid_Pos_B5_14_5H_T4.d
SUBJECT_SAMPLE_FACTORS           	Subject_14	14_5H_8	Gender:M | Time:8 | Sleep Window (Hours):5H	RAW_FILE_NAME=Aqueous_B2_14_5H_T8.d Lipid_Pos_B1_14_5H_T8.d
SUBJECT_SAMPLE_FACTORS           	Subject_14	14_9H_0	Gender:M | Time:zero | Sleep Window (Hours):9H	RAW_FILE_NAME=Aqueous_B8_14_9H_T0A.d Lipid_Pos_B5_14_9H_T0.d
SUBJECT_SAMPLE_FACTORS           	Subject_14	14_9H_12	Gender:M | Time:12 | Sleep Window (Hours):9H	RAW_FILE_NAME=Aqueous_B7_14_9H_T12.d Lipid_Pos_B3_14_9H_T12_B.d
SUBJECT_SAMPLE_FACTORS           	Subject_14	14_9H_16	Gender:M | Time:16 | Sleep Window (Hours):9H	RAW_FILE_NAME=Aqueous_B6_14_9H_T16.d Lipid_Pos_B5_14_9H_T16.d
SUBJECT_SAMPLE_FACTORS           	Subject_14	14_9H_20	Gender:M | Time:20 | Sleep Window (Hours):9H	RAW_FILE_NAME=Aqueous_B4_14_9H_T20B.d Lipid_Pos_B6_14_9H_T20_B.d
SUBJECT_SAMPLE_FACTORS           	Subject_14	14_9H_4	Gender:M | Time:4 | Sleep Window (Hours):9H	RAW_FILE_NAME=Aqueous_B4_14_9H_T4.d Lipid_Pos_B3_14_9H_T4.d
SUBJECT_SAMPLE_FACTORS           	Subject_14	14_9H_8	Gender:M | Time:8 | Sleep Window (Hours):9H	RAW_FILE_NAME=Aqueous_B4_14_9H_T8.d Lipid_Pos_B2_14_9H_T8.d
SUBJECT_SAMPLE_FACTORS           	Subject_14	14_BL_0	Gender:M | Time:zero | Sleep Window (Hours):BL	RAW_FILE_NAME=Aqueous_B8_14_BL_T0.d Lipid_Pos_B2_14_BL_T0.d
SUBJECT_SAMPLE_FACTORS           	Subject_14	14_BL_12	Gender:M | Time:12 | Sleep Window (Hours):BL	RAW_FILE_NAME=Aqueous_B4_14_BL_T12.d Lipid_Pos_B4_14_BL_T12.d
SUBJECT_SAMPLE_FACTORS           	Subject_14	14_BL_16	Gender:M | Time:16 | Sleep Window (Hours):BL	RAW_FILE_NAME=Aqueous_B8_14_BL_T16.d Lipid_Pos_B6_14_BL_T16.d
SUBJECT_SAMPLE_FACTORS           	Subject_14	14_BL_20	Gender:M | Time:20 | Sleep Window (Hours):BL	RAW_FILE_NAME=Aqueous_B9_14_BL_T20A.d Lipid_Pos_B6_14_BL_T20.d
SUBJECT_SAMPLE_FACTORS           	Subject_14	14_BL_4	Gender:M | Time:4 | Sleep Window (Hours):BL	RAW_FILE_NAME=Aqueous_B5_14_BL_T4.d Lipid_Pos_B4_14_BL_T4.d
SUBJECT_SAMPLE_FACTORS           	Subject_14	14_BL_8	Gender:M | Time:8 | Sleep Window (Hours):BL	RAW_FILE_NAME=Aqueous_B8_14_BL_T8.d Lipid_Pos_B7_14_BL_T8.d
SUBJECT_SAMPLE_FACTORS           	Subject_16	16_5H_0	Gender:M | Time:zero | Sleep Window (Hours):5H	RAW_FILE_NAME=Aqueous_B6_16_5H_T0.d Lipid_Pos_B4_16_5H_T0.d
SUBJECT_SAMPLE_FACTORS           	Subject_16	16_5H_12	Gender:M | Time:12 | Sleep Window (Hours):5H	RAW_FILE_NAME=Aqueous_B8_16_5H_T12.d Lipid_Pos_B5_16_5H_T12.d
SUBJECT_SAMPLE_FACTORS           	Subject_16	16_5H_16	Gender:M | Time:16 | Sleep Window (Hours):5H	RAW_FILE_NAME=Aqueous_B2_16_5H_T16.d Lipid_Pos_B4_16_5H_T16_B.d
SUBJECT_SAMPLE_FACTORS           	Subject_16	16_5H_20	Gender:M | Time:20 | Sleep Window (Hours):5H	RAW_FILE_NAME=Aqueous_B9_16_5H_T20.d Lipid_Pos_B5_16_5H_T20.d
SUBJECT_SAMPLE_FACTORS           	Subject_16	16_5H_4	Gender:M | Time:4 | Sleep Window (Hours):5H	RAW_FILE_NAME=Aqueous_B3_16_5H_T4.d Lipid_Pos_B5_16_5H_T4.d
SUBJECT_SAMPLE_FACTORS           	Subject_16	16_5H_8	Gender:M | Time:8 | Sleep Window (Hours):5H	RAW_FILE_NAME=Aqueous_B5_16_5H_T8.d Lipid_Pos_B1_16_5H_T8.d
SUBJECT_SAMPLE_FACTORS           	Subject_16	16_9H_0	Gender:M | Time:zero | Sleep Window (Hours):9H	RAW_FILE_NAME=Aqueous_B3_16_9H_T0.d Lipid_Pos_B1_16_9H_T0.d
SUBJECT_SAMPLE_FACTORS           	Subject_16	16_9H_12	Gender:M | Time:12 | Sleep Window (Hours):9H	RAW_FILE_NAME=Aqueous_B6_16_9H_T12.d Lipid_Pos_B5_16_9H_T12.d
SUBJECT_SAMPLE_FACTORS           	Subject_16	16_9H_4	Gender:M | Time:4 | Sleep Window (Hours):9H	RAW_FILE_NAME=Aqueous_B7_16_9H_T4.d Lipid_Pos_B1_16_9H_T4_B.d
SUBJECT_SAMPLE_FACTORS           	Subject_16	16_9H_8	Gender:M | Time:8 | Sleep Window (Hours):9H	RAW_FILE_NAME=Aqueous_B2_16_9H_T8.d Lipid_Pos_B1_16_9H_T8.d
SUBJECT_SAMPLE_FACTORS           	Subject_16	16_BL_0	Gender:M | Time:zero | Sleep Window (Hours):BL	RAW_FILE_NAME=Aqueous_B2_16_BL_T0.d Lipid_Pos_B5_16_BL_T0_B.d
SUBJECT_SAMPLE_FACTORS           	Subject_16	16_BL_12	Gender:M | Time:12 | Sleep Window (Hours):BL	RAW_FILE_NAME=Aqueous_B9_16_BL_T12.d Lipid_Pos_B4_16_BL_T12.d
SUBJECT_SAMPLE_FACTORS           	Subject_16	16_BL_16	Gender:M | Time:16 | Sleep Window (Hours):BL	RAW_FILE_NAME=Aqueous_B3_16_BL_T16.d Lipid_Pos_B6_16_BL_T16.d
SUBJECT_SAMPLE_FACTORS           	Subject_16	16_BL_20	Gender:M | Time:20 | Sleep Window (Hours):BL	RAW_FILE_NAME=Aqueous_B6_16_BL_T20.d Lipid_Pos_B1_16_BL_T20.d
SUBJECT_SAMPLE_FACTORS           	Subject_16	16_BL_4	Gender:M | Time:4 | Sleep Window (Hours):BL	RAW_FILE_NAME=Aqueous_B2_16_BL_T4.d Lipid_Pos_B4_16_BL_T4.d
SUBJECT_SAMPLE_FACTORS           	Subject_16	16_BL_8	Gender:M | Time:8 | Sleep Window (Hours):BL	RAW_FILE_NAME=Aqueous_B3_16_BL_T8.d Lipid_Pos_B6_16_BL_T8.d
SUBJECT_SAMPLE_FACTORS           	Subject_2	2_5H_0	Gender:M | Time:zero | Sleep Window (Hours):5H	RAW_FILE_NAME=Aqueous_B6_02_5H_T0.d Lipid_Pos_B2_02_5H_T0_B.d
SUBJECT_SAMPLE_FACTORS           	Subject_2	2_5H_12	Gender:M | Time:12 | Sleep Window (Hours):5H	RAW_FILE_NAME=Aqueous_B6_02_5H_T12.d Lipid_Pos_B2_02_5H_T12.d
SUBJECT_SAMPLE_FACTORS           	Subject_2	2_5H_16	Gender:M | Time:16 | Sleep Window (Hours):5H	RAW_FILE_NAME=Aqueous_B2_02_5H_T16A.d Lipid_Pos_B1_02_5H_T16.d
SUBJECT_SAMPLE_FACTORS           	Subject_2	2_5H_20	Gender:M | Time:20 | Sleep Window (Hours):5H	RAW_FILE_NAME=Aqueous_B3_02_5H_T20.d Lipid_Pos_B4_02_5H_T20.d
SUBJECT_SAMPLE_FACTORS           	Subject_2	2_5H_4	Gender:M | Time:4 | Sleep Window (Hours):5H	RAW_FILE_NAME=Aqueous_B6_02_5H_T4.d Lipid_Pos_B5_02_5H_T4.d
SUBJECT_SAMPLE_FACTORS           	Subject_2	2_5H_8	Gender:M | Time:8 | Sleep Window (Hours):5H	RAW_FILE_NAME=Aqueous_B5_02_5H_T8.d Lipid_Pos_B5_02_5H_T8.d
SUBJECT_SAMPLE_FACTORS           	Subject_2	2_9H_0	Gender:M | Time:zero | Sleep Window (Hours):9H	RAW_FILE_NAME=Aqueous_B4_02_9H_T0_RR.d Lipid_Pos_B2_02_9H_T0.d
SUBJECT_SAMPLE_FACTORS           	Subject_2	2_9H_12	Gender:M | Time:12 | Sleep Window (Hours):9H	RAW_FILE_NAME=Aqueous_B5_02_9H_T12_RR.d Lipid_Pos_B5_02_9H_T12_B.d
SUBJECT_SAMPLE_FACTORS           	Subject_2	2_9H_4	Gender:M | Time:4 | Sleep Window (Hours):9H	RAW_FILE_NAME=Aqueous_B9_02_9H_T4.d Lipid_Pos_B6_02_9H_T4.d
SUBJECT_SAMPLE_FACTORS           	Subject_2	2_9H_8	Gender:M | Time:8 | Sleep Window (Hours):9H	RAW_FILE_NAME=Aqueous_B8_02_9H_T8.d Lipid_Pos_B1_02_9H_T8.d
SUBJECT_SAMPLE_FACTORS           	Subject_4	4_5H_0	Gender:M | Time:zero | Sleep Window (Hours):5H	RAW_FILE_NAME=Aqueous_B4_04_5H_T0A.d Lipid_Pos_B7_04_5H_T0.d
SUBJECT_SAMPLE_FACTORS           	Subject_4	4_5H_12	Gender:M | Time:12 | Sleep Window (Hours):5H	RAW_FILE_NAME=Aqueous_B4_04_5H_T12.d Lipid_Pos_B4_04_5H_T12.d
SUBJECT_SAMPLE_FACTORS           	Subject_4	4_5H_16	Gender:M | Time:16 | Sleep Window (Hours):5H	RAW_FILE_NAME=Aqueous_B9_04_5H_T16.d Lipid_Pos_B3_04_5H_T16.d
SUBJECT_SAMPLE_FACTORS           	Subject_4	4_5H_20	Gender:M | Time:20 | Sleep Window (Hours):5H	RAW_FILE_NAME=Aqueous_B2_04_5H_T20.d Lipid_Pos_B4_04_5H_T20.d
SUBJECT_SAMPLE_FACTORS           	Subject_4	4_5H_4	Gender:M | Time:4 | Sleep Window (Hours):5H	RAW_FILE_NAME=Aqueous_B5_04_5H_T4.d Lipid_Pos_B2_04_5H_T4.d
SUBJECT_SAMPLE_FACTORS           	Subject_4	4_5H_8	Gender:M | Time:8 | Sleep Window (Hours):5H	RAW_FILE_NAME=Aqueous_B9_04_5H_T8.d Lipid_Pos_B2_04_5H_T8.d
SUBJECT_SAMPLE_FACTORS           	Subject_4	4_9H_0	Gender:M | Time:zero | Sleep Window (Hours):9H	RAW_FILE_NAME=Aqueous_B2_04_9H_T0.d Lipid_Pos_B6_04_9H_T0.d
SUBJECT_SAMPLE_FACTORS           	Subject_4	4_9H_12	Gender:M | Time:12 | Sleep Window (Hours):9H	RAW_FILE_NAME=Aqueous_B7_04_9H_T12.d Lipid_Pos_B6_04_9H_T12.d
SUBJECT_SAMPLE_FACTORS           	Subject_4	4_9H_16	Gender:M | Time:16 | Sleep Window (Hours):9H	RAW_FILE_NAME=Aqueous_B9_04_9H_T16.d Lipid_Pos_B1_04_9H_T16.d
SUBJECT_SAMPLE_FACTORS           	Subject_4	4_9H_20	Gender:M | Time:20 | Sleep Window (Hours):9H	RAW_FILE_NAME=Aqueous_B7_04_9H_T20.d Lipid_Pos_B4_04_9H_T20_B.d
SUBJECT_SAMPLE_FACTORS           	Subject_4	4_9H_4	Gender:M | Time:4 | Sleep Window (Hours):9H	RAW_FILE_NAME=Aqueous_B6_04_9H_T4.d Lipid_Pos_B5_04_9H_T4.d
SUBJECT_SAMPLE_FACTORS           	Subject_4	4_9H_8	Gender:M | Time:8 | Sleep Window (Hours):9H	RAW_FILE_NAME=Aqueous_B2_04_9H_T8.d Lipid_Pos_B3_04_9H_T8_B.d
SUBJECT_SAMPLE_FACTORS           	Subject_4	4_BL_0	Gender:M | Time:zero | Sleep Window (Hours):BL	RAW_FILE_NAME=Aqueous_B3_04_BL_T0B.d Lipid_Pos_B3_04_BL_T0.d
SUBJECT_SAMPLE_FACTORS           	Subject_4	4_BL_12	Gender:M | Time:12 | Sleep Window (Hours):BL	RAW_FILE_NAME=Aqueous_B7_04_BL_T12.d Lipid_Pos_B7_04_BL_T12.d
SUBJECT_SAMPLE_FACTORS           	Subject_4	4_BL_16	Gender:M | Time:16 | Sleep Window (Hours):BL	RAW_FILE_NAME=Aqueous_B4_04_BL_T16.d Lipid_Pos_B6_04_BL_T16.d
SUBJECT_SAMPLE_FACTORS           	Subject_4	4_BL_20	Gender:M | Time:20 | Sleep Window (Hours):BL	RAW_FILE_NAME=Aqueous_B6_04_BL_T20.d Lipid_Pos_B7_04_BL_T20.d
SUBJECT_SAMPLE_FACTORS           	Subject_4	4_BL_4	Gender:M | Time:4 | Sleep Window (Hours):BL	RAW_FILE_NAME=Aqueous_B4_04_BL_T4.d Lipid_Pos_B3_04_BL_T4.d
SUBJECT_SAMPLE_FACTORS           	Subject_4	4_BL_8	Gender:M | Time:8 | Sleep Window (Hours):BL	RAW_FILE_NAME=Aqueous_B2_04_BL_T8.d Lipid_Pos_B4_04_BL_T8.d
SUBJECT_SAMPLE_FACTORS           	Subject_8	8_9H_0	Gender:M | Time:zero | Sleep Window (Hours):9H	RAW_FILE_NAME=Aqueous_B8_08_9H_T0.d Lipid_Pos_B4_08_9H_T0.d
SUBJECT_SAMPLE_FACTORS           	Subject_8	8_9H_12	Gender:M | Time:12 | Sleep Window (Hours):9H	RAW_FILE_NAME=Aqueous_B5_08_9H_T12.d Lipid_Pos_B5_08_9H_T12.d
SUBJECT_SAMPLE_FACTORS           	Subject_8	8_9H_16	Gender:M | Time:16 | Sleep Window (Hours):9H	RAW_FILE_NAME=Aqueous_B2_08_9H_T16.d Lipid_Pos_B4_08_9H_T16_B.d
SUBJECT_SAMPLE_FACTORS           	Subject_8	8_9H_20	Gender:M | Time:20 | Sleep Window (Hours):9H	RAW_FILE_NAME=Aqueous_B6_08_9H_T20.d Lipid_Pos_B5_08_9H_T20.d
SUBJECT_SAMPLE_FACTORS           	Subject_8	8_9H_4	Gender:M | Time:4 | Sleep Window (Hours):9H	RAW_FILE_NAME=Aqueous_B4_08_9H_T4.d Lipid_Pos_B2_08_9H_T4.d
SUBJECT_SAMPLE_FACTORS           	Subject_9	9_5H_0	Gender:M | Time:zero | Sleep Window (Hours):5H	RAW_FILE_NAME=Aqueous_B8_09_5H_T0_RS.d Lipid_Pos_B2_09_5H_T0.d
SUBJECT_SAMPLE_FACTORS           	Subject_9	9_5H_12	Gender:M | Time:12 | Sleep Window (Hours):5H	RAW_FILE_NAME=Aqueous_B2_09_5H_T12.d Lipid_Pos_B7_09_5H_T12.d
SUBJECT_SAMPLE_FACTORS           	Subject_9	9_5H_16	Gender:M | Time:16 | Sleep Window (Hours):5H	RAW_FILE_NAME=Aqueous_B3_09_5H_T16.d Lipid_Pos_B3_09_5H_T16.d
SUBJECT_SAMPLE_FACTORS           	Subject_9	9_5H_20	Gender:M | Time:20 | Sleep Window (Hours):5H	RAW_FILE_NAME=Aqueous_B3_09_5H_T20_RRB4.d Lipid_Pos_B6_09_5H_T20.d
SUBJECT_SAMPLE_FACTORS           	Subject_9	9_5H_4	Gender:M | Time:4 | Sleep Window (Hours):5H	RAW_FILE_NAME=Aqueous_B4_09_5H_T4.d Lipid_Pos_B6_09_5H_T4.d
SUBJECT_SAMPLE_FACTORS           	Subject_9	9_5H_8	Gender:M | Time:8 | Sleep Window (Hours):5H	RAW_FILE_NAME=Aqueous_B4_09_5H_T8.d Lipid_Pos_B5_09_5H_T8.d
SUBJECT_SAMPLE_FACTORS           	Subject_9	9_9H_0	Gender:M | Time:zero | Sleep Window (Hours):9H	RAW_FILE_NAME=Aqueous_B9_09_9H_T0.d Lipid_Pos_B3_09_9H_T0.d
SUBJECT_SAMPLE_FACTORS           	Subject_9	9_9H_12	Gender:M | Time:12 | Sleep Window (Hours):9H	RAW_FILE_NAME=Aqueous_B6_09_9H_T12.d Lipid_Pos_B5_09_9H_T12.d
SUBJECT_SAMPLE_FACTORS           	Subject_9	9_9H_16	Gender:M | Time:16 | Sleep Window (Hours):9H	RAW_FILE_NAME=Aqueous_B2_09_9H_T16.d Lipid_Pos_B3_09_9H_T16.d
SUBJECT_SAMPLE_FACTORS           	Subject_9	9_9H_20	Gender:M | Time:20 | Sleep Window (Hours):9H	RAW_FILE_NAME=Aqueous_B3_09_9H_T20.d Lipid_Pos_B6_09_9H_T20.d
SUBJECT_SAMPLE_FACTORS           	Subject_9	9_9H_4	Gender:M | Time:4 | Sleep Window (Hours):9H	RAW_FILE_NAME=Aqueous_B2_09_9H_T4.d Lipid_Pos_B3_09_9H_T4.d
SUBJECT_SAMPLE_FACTORS           	Subject_9	9_9H_8	Gender:M | Time:8 | Sleep Window (Hours):9H	RAW_FILE_NAME=Aqueous_B4_09_9H_T8.d Lipid_Pos_B2_09_9H_T8_B.d
SUBJECT_SAMPLE_FACTORS           	Subject_9	9_BL_0	Gender:M | Time:zero | Sleep Window (Hours):BL	RAW_FILE_NAME=Aqueous_B7_09_BL_T0.d Lipid_Pos_B3_09_BL_T0.d
SUBJECT_SAMPLE_FACTORS           	Subject_9	9_BL_12	Gender:M | Time:12 | Sleep Window (Hours):BL	RAW_FILE_NAME=Aqueous_B8_09_BL_T12A.d Lipid_Pos_B1_09_BL_T12.d
SUBJECT_SAMPLE_FACTORS           	Subject_9	9_BL_16	Gender:M | Time:16 | Sleep Window (Hours):BL	RAW_FILE_NAME=Aqueous_B7_09_BL_T16.d Lipid_Pos_B2_09_BL_T16.d
SUBJECT_SAMPLE_FACTORS           	Subject_9	9_BL_20	Gender:M | Time:20 | Sleep Window (Hours):BL	RAW_FILE_NAME=Aqueous_B8_09_BL_T20A.d Lipid_Pos_B2_09_BL_T20_B.d
SUBJECT_SAMPLE_FACTORS           	Subject_9	9_BL_4	Gender:M | Time:4 | Sleep Window (Hours):BL	RAW_FILE_NAME=Aqueous_B9_09_BL_T4.d Lipid_Pos_B2_09_BL_T4.d
SUBJECT_SAMPLE_FACTORS           	Subject_9	9_BL_8	Gender:M | Time:8 | Sleep Window (Hours):BL	RAW_FILE_NAME=Aqueous_B5_09_BL_T8A.d Lipid_Pos_B4_09_BL_T8.d
SUBJECT_SAMPLE_FACTORS           	Subject_10	10_5H_12	Gender:F | Time:12 | Sleep Window (Hours):5H	RAW_FILE_NAME=Aqueous_B7_10_5H_T12.d Lipid_Pos_B5_10_5H_T12.d
SUBJECT_SAMPLE_FACTORS           	Subject_10	10_5H_16	Gender:F | Time:16 | Sleep Window (Hours):5H	RAW_FILE_NAME=Aqueous_B9_10_5H_T16.d Lipid_Pos_B7_10_5H_T16.d
SUBJECT_SAMPLE_FACTORS           	Subject_10	10_5H_4	Gender:F | Time:4 | Sleep Window (Hours):5H	RAW_FILE_NAME=Aqueous_B9_10_5H_T4.d Lipid_Pos_B2_10_5H_T4.d
SUBJECT_SAMPLE_FACTORS           	Subject_10	10_5H_8	Gender:F | Time:8 | Sleep Window (Hours):5H	RAW_FILE_NAME=Aqueous_B6_10_5H_T8.d Lipid_Pos_B2_10_5H_T8.d
SUBJECT_SAMPLE_FACTORS           	Subject_10	10_9H_12	Gender:F | Time:12 | Sleep Window (Hours):9H	RAW_FILE_NAME=Aqueous_B8_10_9H_T12.d Lipid_Pos_B3_10_9H_T12.d
SUBJECT_SAMPLE_FACTORS           	Subject_10	10_9H_16	Gender:F | Time:16 | Sleep Window (Hours):9H	RAW_FILE_NAME=Aqueous_B7_10_9H_T16.d Lipid_Pos_B7_10_9H_T16.d
SUBJECT_SAMPLE_FACTORS           	Subject_10	10_9H_20	Gender:F | Time:20 | Sleep Window (Hours):9H	RAW_FILE_NAME=Aqueous_B5_10_9H_T20.d Lipid_Pos_B6_10_9H_T20.d
SUBJECT_SAMPLE_FACTORS           	Subject_10	10_9H_4	Gender:F | Time:4 | Sleep Window (Hours):9H	RAW_FILE_NAME=Aqueous_B3_10_9H_T4.d Lipid_Pos_B7_10_9H_T4.d
SUBJECT_SAMPLE_FACTORS           	Subject_10	10_9H_8	Gender:F | Time:8 | Sleep Window (Hours):9H	RAW_FILE_NAME=Aqueous_B6_10_9H_T8.d Lipid_Pos_B2_10_9H_T8.d
SUBJECT_SAMPLE_FACTORS           	Subject_10	10_BL_0	Gender:F | Time:zero | Sleep Window (Hours):BL	RAW_FILE_NAME=Aqueous_B2_10_BL_T0.d Lipid_Pos_B3_10_BL_T0_B.d
SUBJECT_SAMPLE_FACTORS           	Subject_10	10_BL_12	Gender:F | Time:12 | Sleep Window (Hours):BL	RAW_FILE_NAME=Aqueous_B6_10_BL_T12.d Lipid_Pos_B2_10_BL_T12.d
SUBJECT_SAMPLE_FACTORS           	Subject_10	10_BL_16	Gender:F | Time:16 | Sleep Window (Hours):BL	RAW_FILE_NAME=Aqueous_B8_10_BL_T16.d Lipid_Pos_B6_10_BL_T16.d
SUBJECT_SAMPLE_FACTORS           	Subject_10	10_BL_20	Gender:F | Time:20 | Sleep Window (Hours):BL	RAW_FILE_NAME=Aqueous_B9_10_BL_T20A.d Lipid_Pos_B6_10_BL_T20.d
SUBJECT_SAMPLE_FACTORS           	Subject_10	10_BL_4	Gender:F | Time:4 | Sleep Window (Hours):BL	RAW_FILE_NAME=Aqueous_B9_10_BL_T4.d Lipid_Pos_B3_10_BL_T4.d
SUBJECT_SAMPLE_FACTORS           	Subject_10	10_BL_8	Gender:F | Time:8 | Sleep Window (Hours):BL	RAW_FILE_NAME=Aqueous_B7_10_BL_T8.d Lipid_Pos_B2_10_BL_T8.d
SUBJECT_SAMPLE_FACTORS           	Subject_12	12_5H_0	Gender:F | Time:zero | Sleep Window (Hours):5H	RAW_FILE_NAME=Aqueous_B4_12_5H_T0.d Lipid_Pos_B3_12_5H_T0.d
SUBJECT_SAMPLE_FACTORS           	Subject_12	12_5H_12	Gender:F | Time:12 | Sleep Window (Hours):5H	RAW_FILE_NAME=Aqueous_B5_12_5H_T12A.d Lipid_Pos_B1_12_5H_T12_B.d
SUBJECT_SAMPLE_FACTORS           	Subject_12	12_5H_16	Gender:F | Time:16 | Sleep Window (Hours):5H	RAW_FILE_NAME=Aqueous_B8_12_5H_T16.d Lipid_Pos_B2_12_5H_T16_B.d
SUBJECT_SAMPLE_FACTORS           	Subject_12	12_5H_20	Gender:F | Time:20 | Sleep Window (Hours):5H	RAW_FILE_NAME=Aqueous_B6_12_5H_T20.d Lipid_Pos_B3_12_5H_T20.d
SUBJECT_SAMPLE_FACTORS           	Subject_12	12_5H_4	Gender:F | Time:4 | Sleep Window (Hours):5H	RAW_FILE_NAME=Aqueous_B2_12_5H_T4.d Lipid_Pos_B7_12_5H_T4.d
SUBJECT_SAMPLE_FACTORS           	Subject_12	12_5H_8	Gender:F | Time:8 | Sleep Window (Hours):5H	RAW_FILE_NAME=Aqueous_B7_12_5H_T8.d Lipid_Pos_B5_12_5H_T8.d
SUBJECT_SAMPLE_FACTORS           	Subject_12	12_9H_0	Gender:F | Time:zero | Sleep Window (Hours):9H	RAW_FILE_NAME=Aqueous_B3_12_9H_T0.d Lipid_Pos_B4_12_9H_T0.d
SUBJECT_SAMPLE_FACTORS           	Subject_12	12_9H_12	Gender:F | Time:12 | Sleep Window (Hours):9H	RAW_FILE_NAME=Aqueous_B4_12_9H_T12_RR.d Lipid_Pos_B4_12_9H_T12.d
SUBJECT_SAMPLE_FACTORS           	Subject_12	12_9H_20	Gender:F | Time:20 | Sleep Window (Hours):9H	RAW_FILE_NAME=Aqueous_B8_12_9H_T20.d Lipid_Pos_B5_12_9H_T20.d
SUBJECT_SAMPLE_FACTORS           	Subject_12	12_9H_4	Gender:F | Time:4 | Sleep Window (Hours):9H	RAW_FILE_NAME=Aqueous_B7_12_9H_T4.d Lipid_Pos_B2_12_9H_T4.d
SUBJECT_SAMPLE_FACTORS           	Subject_12	12_9H_8	Gender:F | Time:8 | Sleep Window (Hours):9H	RAW_FILE_NAME=Aqueous_B7_12_9H_T8.d Lipid_Pos_B2_12_9H_T8.d
SUBJECT_SAMPLE_FACTORS           	Subject_12	12_BL_0	Gender:F | Time:zero | Sleep Window (Hours):BL	RAW_FILE_NAME=Aqueous_B6_12_BL_T0.d Lipid_Pos_B3_12_BL_T0.d
SUBJECT_SAMPLE_FACTORS           	Subject_12	12_BL_12	Gender:F | Time:12 | Sleep Window (Hours):BL	RAW_FILE_NAME=Aqueous_B3_12_BL_T12.d Lipid_Pos_B7_12_BL_T12.d
SUBJECT_SAMPLE_FACTORS           	Subject_12	12_BL_16	Gender:F | Time:16 | Sleep Window (Hours):BL	RAW_FILE_NAME=Aqueous_B8_12_BL_T16.d Lipid_Pos_B2_12_BL_T16.d
SUBJECT_SAMPLE_FACTORS           	Subject_12	12_BL_20	Gender:F | Time:20 | Sleep Window (Hours):BL	RAW_FILE_NAME=Aqueous_B8_12_BL_T20.d Lipid_Pos_B7_12_BL_T20.d
SUBJECT_SAMPLE_FACTORS           	Subject_12	12_BL_4	Gender:F | Time:4 | Sleep Window (Hours):BL	RAW_FILE_NAME=Aqueous_B3_12_BL_T4.d Lipid_Pos_B5_12_BL_T4.d
SUBJECT_SAMPLE_FACTORS           	Subject_12	12_BL_8	Gender:F | Time:8 | Sleep Window (Hours):BL	RAW_FILE_NAME=Aqueous_B6_12_BL_T8.d Lipid_Pos_B2_12_BL_T8.d
SUBJECT_SAMPLE_FACTORS           	Subject_13	13_9H_0	Gender:F | Time:zero | Sleep Window (Hours):9H	RAW_FILE_NAME=Aqueous_B9_13_9H_T0.d Lipid_Pos_B7_13_9H_T0_B.d
SUBJECT_SAMPLE_FACTORS           	Subject_13	13_9H_12	Gender:F | Time:12 | Sleep Window (Hours):9H	RAW_FILE_NAME=Aqueous_B2_13_9H_T12A.d Lipid_Pos_B1_13_9H_T12_B.d
SUBJECT_SAMPLE_FACTORS           	Subject_13	13_9H_20	Gender:F | Time:20 | Sleep Window (Hours):9H	RAW_FILE_NAME=Aqueous_B2_13_9H_T20.d Lipid_Pos_B2_13_9H_T20.d
SUBJECT_SAMPLE_FACTORS           	Subject_13	13_9H_4	Gender:F | Time:4 | Sleep Window (Hours):9H	RAW_FILE_NAME=Aqueous_B7_13_9H_T4.d Lipid_Pos_B6_13_9H_T4.d
SUBJECT_SAMPLE_FACTORS           	Subject_13	13_9H_8	Gender:F | Time:8 | Sleep Window (Hours):9H	RAW_FILE_NAME=Aqueous_B7_13_9H_T8.d Lipid_Pos_B7_13_9H_T8.d
SUBJECT_SAMPLE_FACTORS           	Subject_13	13_BL_0	Gender:F | Time:zero | Sleep Window (Hours):BL	RAW_FILE_NAME=Aqueous_B5_13_BL_T0.d Lipid_Pos_B5_13_BL_T0.d
SUBJECT_SAMPLE_FACTORS           	Subject_13	13_BL_12	Gender:F | Time:12 | Sleep Window (Hours):BL	RAW_FILE_NAME=Aqueous_B4_13_BL_T12.d Lipid_Pos_B7_13_BL_T12.d
SUBJECT_SAMPLE_FACTORS           	Subject_13	13_BL_4	Gender:F | Time:4 | Sleep Window (Hours):BL	RAW_FILE_NAME=Aqueous_B5_13_BL_T4B.d Lipid_Pos_B1_13_BL_T4.d
SUBJECT_SAMPLE_FACTORS           	Subject_15	15_5H_0	Gender:F | Time:zero | Sleep Window (Hours):5H	RAW_FILE_NAME=Aqueous_B2_15_5H_T0.d Lipid_Pos_B3_15_5H_T0.d
SUBJECT_SAMPLE_FACTORS           	Subject_15	15_5H_12	Gender:F | Time:12 | Sleep Window (Hours):5H	RAW_FILE_NAME=Aqueous_B9_15_5H_T12.d Lipid_Pos_B6_15_5H_T12.d
SUBJECT_SAMPLE_FACTORS           	Subject_15	15_5H_16	Gender:F | Time:16 | Sleep Window (Hours):5H	RAW_FILE_NAME=Aqueous_B5_15_5H_T16.d Lipid_Pos_B5_15_5H_T16.d
SUBJECT_SAMPLE_FACTORS           	Subject_15	15_5H_20	Gender:F | Time:20 | Sleep Window (Hours):5H	RAW_FILE_NAME=Aqueous_B6_15_5H_T20.d Lipid_Pos_B1_15_5H_T20.d
SUBJECT_SAMPLE_FACTORS           	Subject_15	15_5H_4	Gender:F | Time:4 | Sleep Window (Hours):5H	RAW_FILE_NAME=Aqueous_B3_15_5H_T4A.d Lipid_Pos_B1_15_5H_T4.d
SUBJECT_SAMPLE_FACTORS           	Subject_15	15_5H_8	Gender:F | Time:8 | Sleep Window (Hours):5H	RAW_FILE_NAME=Aqueous_B8_15_5H_T8.d Lipid_Pos_B3_15_5H_T8.d
SUBJECT_SAMPLE_FACTORS           	Subject_15	15_9H_0	Gender:F | Time:zero | Sleep Window (Hours):9H	RAW_FILE_NAME=Aqueous_B2_15_9H_T0.d Lipid_Pos_B4_15_9H_T0.d
SUBJECT_SAMPLE_FACTORS           	Subject_15	15_9H_12	Gender:F | Time:12 | Sleep Window (Hours):9H	RAW_FILE_NAME=Aqueous_B7_15_9H_T12.d Lipid_Pos_B6_15_9H_T12.d
SUBJECT_SAMPLE_FACTORS           	Subject_15	15_9H_16	Gender:F | Time:16 | Sleep Window (Hours):9H	RAW_FILE_NAME=Aqueous_B9_15_9H_T16.d Lipid_Pos_B6_15_9H_T16.d
SUBJECT_SAMPLE_FACTORS           	Subject_15	15_9H_20	Gender:F | Time:20 | Sleep Window (Hours):9H	RAW_FILE_NAME=Aqueous_B9_15_9H_T20.d Lipid_Pos_B7_15_9H_T20.d
SUBJECT_SAMPLE_FACTORS           	Subject_15	15_9H_4	Gender:F | Time:4 | Sleep Window (Hours):9H	RAW_FILE_NAME=Aqueous_B9_15_9H_T4.d Lipid_Pos_B1_15_9H_T4.d
SUBJECT_SAMPLE_FACTORS           	Subject_15	15_9H_8	Gender:F | Time:8 | Sleep Window (Hours):9H	RAW_FILE_NAME=Aqueous_B9_15_9H_T8.d Lipid_Pos_B1_15_9H_T8.d
SUBJECT_SAMPLE_FACTORS           	Subject_15	15_BL_0	Gender:F | Time:zero | Sleep Window (Hours):BL	RAW_FILE_NAME=Aqueous_B7_15_BL_T0.d Lipid_Pos_B6_15_BL_T0.d
SUBJECT_SAMPLE_FACTORS           	Subject_15	15_BL_12	Gender:F | Time:12 | Sleep Window (Hours):BL	RAW_FILE_NAME=Aqueous_B7_15_BL_T12.d Lipid_Pos_B2_15_BL_T12.d
SUBJECT_SAMPLE_FACTORS           	Subject_15	15_BL_16	Gender:F | Time:16 | Sleep Window (Hours):BL	RAW_FILE_NAME=Aqueous_B3_15_BL_T16.d Lipid_Pos_B6_15_BL_T16.d
SUBJECT_SAMPLE_FACTORS           	Subject_15	15_BL_20	Gender:F | Time:20 | Sleep Window (Hours):BL	RAW_FILE_NAME=Aqueous_B5_15_BL_T20.d Lipid_Pos_B7_15_BL_T20.d
SUBJECT_SAMPLE_FACTORS           	Subject_15	15_BL_4	Gender:F | Time:4 | Sleep Window (Hours):BL	RAW_FILE_NAME=Aqueous_B6_15_BL_T4.d Lipid_Pos_B4_15_BL_T4.d
SUBJECT_SAMPLE_FACTORS           	Subject_15	15_BL_8	Gender:F | Time:8 | Sleep Window (Hours):BL	RAW_FILE_NAME=Aqueous_B6_15_BL_T8B.d Lipid_Pos_B2_15_BL_T8.d
SUBJECT_SAMPLE_FACTORS           	Subject_17	17_5H_0	Gender:F | Time:zero | Sleep Window (Hours):5H	RAW_FILE_NAME=Aqueous_B3_17_5H_T0.d Lipid_Pos_B5_17_5H_T0_B.d
SUBJECT_SAMPLE_FACTORS           	Subject_17	17_5H_12	Gender:F | Time:12 | Sleep Window (Hours):5H	RAW_FILE_NAME=Aqueous_B9_17_5H_T12.d Lipid_Pos_B6_17_5H_T12.d
SUBJECT_SAMPLE_FACTORS           	Subject_17	17_5H_16	Gender:F | Time:16 | Sleep Window (Hours):5H	RAW_FILE_NAME=Aqueous_B7_17_5H_T16.d Lipid_Pos_B5_17_5H_T16.d
SUBJECT_SAMPLE_FACTORS           	Subject_17	17_5H_20	Gender:F | Time:20 | Sleep Window (Hours):5H	RAW_FILE_NAME=Aqueous_B4_17_5H_T20.d Lipid_Pos_B3_17_5H_T20.d
SUBJECT_SAMPLE_FACTORS           	Subject_17	17_5H_4	Gender:F | Time:4 | Sleep Window (Hours):5H	RAW_FILE_NAME=Aqueous_B9_17_5H_T4.d Lipid_Pos_B2_17_5H_T4.d
SUBJECT_SAMPLE_FACTORS           	Subject_17	17_5H_8	Gender:F | Time:8 | Sleep Window (Hours):5H	RAW_FILE_NAME=Aqueous_B4_17_5H_T8.d Lipid_Pos_B2_17_5H_T8.d
SUBJECT_SAMPLE_FACTORS           	Subject_17	17_9H_0	Gender:F | Time:zero | Sleep Window (Hours):9H	RAW_FILE_NAME=Aqueous_B5_17_9H_T0.d Lipid_Pos_B6_17_9H_T0_B.d
SUBJECT_SAMPLE_FACTORS           	Subject_17	17_9H_12	Gender:F | Time:12 | Sleep Window (Hours):9H	RAW_FILE_NAME=Aqueous_B7_17_9H_T12.d Lipid_Pos_B4_17_9H_T12.d
SUBJECT_SAMPLE_FACTORS           	Subject_17	17_9H_16	Gender:F | Time:16 | Sleep Window (Hours):9H	RAW_FILE_NAME=Aqueous_B6_17_9H_T16.d Lipid_Pos_B4_17_9H_T16.d
SUBJECT_SAMPLE_FACTORS           	Subject_17	17_9H_20	Gender:F | Time:20 | Sleep Window (Hours):9H	RAW_FILE_NAME=Aqueous_B3_17_9H_T20.d Lipid_Pos_B6_17_9H_T20_B.d
SUBJECT_SAMPLE_FACTORS           	Subject_17	17_9H_4	Gender:F | Time:4 | Sleep Window (Hours):9H	RAW_FILE_NAME=Aqueous_B7_17_9H_T4.d Lipid_Pos_B3_17_9H_T4.d
SUBJECT_SAMPLE_FACTORS           	Subject_17	17_9H_8	Gender:F | Time:8 | Sleep Window (Hours):9H	RAW_FILE_NAME=Aqueous_B9_17_9H_T8.d Lipid_Pos_B1_17_9H_T8.d
SUBJECT_SAMPLE_FACTORS           	Subject_17	17_BL_12	Gender:F | Time:12 | Sleep Window (Hours):BL	RAW_FILE_NAME=Aqueous_B6_17_BL_T12.d Lipid_Pos_B3_17_BL_T12.d
SUBJECT_SAMPLE_FACTORS           	Subject_17	17_BL_16	Gender:F | Time:16 | Sleep Window (Hours):BL	RAW_FILE_NAME=Aqueous_B4_17_BL_T16A.d Lipid_Pos_B1_17_BL_T16.d
SUBJECT_SAMPLE_FACTORS           	Subject_17	17_BL_20	Gender:F | Time:20 | Sleep Window (Hours):BL	RAW_FILE_NAME=Aqueous_B8_17_BL_T20.d Lipid_Pos_B1_17_BL_T20.d
SUBJECT_SAMPLE_FACTORS           	Subject_17	17_BL_4	Gender:F | Time:4 | Sleep Window (Hours):BL	RAW_FILE_NAME=Aqueous_B2_17_BL_T4.d Lipid_Pos_B6_17_BL_T4.d
SUBJECT_SAMPLE_FACTORS           	Subject_17	17_BL_8	Gender:F | Time:8 | Sleep Window (Hours):BL	RAW_FILE_NAME=Aqueous_B6_17_BL_T8.d Lipid_Pos_B4_17_BL_T8.d
SUBJECT_SAMPLE_FACTORS           	Subject_3	3_5H_0	Gender:F | Time:zero | Sleep Window (Hours):5H	RAW_FILE_NAME=Aqueous_B2_03_5H_T0.d Lipid_Pos_B4_03_5H_T0_B.d
SUBJECT_SAMPLE_FACTORS           	Subject_3	3_5H_12	Gender:F | Time:12 | Sleep Window (Hours):5H	RAW_FILE_NAME=Aqueous_B6_03_5H_T12B.d Lipid_Pos_B2_03_5H_T12.d
SUBJECT_SAMPLE_FACTORS           	Subject_3	3_5H_16	Gender:F | Time:16 | Sleep Window (Hours):5H	RAW_FILE_NAME=Aqueous_B6_03_5H_T16.d Lipid_Pos_B1_03_5H_T16.d
SUBJECT_SAMPLE_FACTORS           	Subject_3	3_5H_20	Gender:F | Time:20 | Sleep Window (Hours):5H	RAW_FILE_NAME=Aqueous_B7_03_5H_T20.d Lipid_Pos_B4_03_5H_T20.d
SUBJECT_SAMPLE_FACTORS           	Subject_3	3_5H_4	Gender:F | Time:4 | Sleep Window (Hours):5H	RAW_FILE_NAME=Aqueous_B2_03_5H_T4A.d Lipid_Pos_B6_03_5H_T4.d
SUBJECT_SAMPLE_FACTORS           	Subject_3	3_5H_8	Gender:F | Time:8 | Sleep Window (Hours):5H	RAW_FILE_NAME=Aqueous_B9_03_5H_T8.d Lipid_Pos_B5_03_5H_T8.d
SUBJECT_SAMPLE_FACTORS           	Subject_3	3_9H_12	Gender:F | Time:12 | Sleep Window (Hours):9H	RAW_FILE_NAME=Aqueous_B9_03_9H_T12.d Lipid_Pos_B3_03_9H_T12.d
SUBJECT_SAMPLE_FACTORS           	Subject_3	3_9H_16	Gender:F | Time:16 | Sleep Window (Hours):9H	RAW_FILE_NAME=Aqueous_B7_03_9H_T16.d Lipid_Pos_B1_03_9H_T16.d
SUBJECT_SAMPLE_FACTORS           	Subject_3	3_9H_20	Gender:F | Time:20 | Sleep Window (Hours):9H	RAW_FILE_NAME=Aqueous_B2_03_9H_T20.d Lipid_Pos_B3_03_9H_T20.d
SUBJECT_SAMPLE_FACTORS           	Subject_3	3_9H_4	Gender:F | Time:4 | Sleep Window (Hours):9H	RAW_FILE_NAME=Aqueous_B6_03_9H_T4.d Lipid_Pos_B4_03_9H_T4.d
SUBJECT_SAMPLE_FACTORS           	Subject_3	3_9H_8	Gender:F | Time:8 | Sleep Window (Hours):9H	RAW_FILE_NAME=Aqueous_B9_03_9H_T8.d Lipid_Pos_B1_03_9H_T8.d
SUBJECT_SAMPLE_FACTORS           	Subject_3	3_BL_0	Gender:F | Time:zero | Sleep Window (Hours):BL	RAW_FILE_NAME=Aqueous_B6_03_BL_T0.d Lipid_Pos_B6_03_BL_T0.d
SUBJECT_SAMPLE_FACTORS           	Subject_3	3_BL_12	Gender:F | Time:12 | Sleep Window (Hours):BL	RAW_FILE_NAME=Aqueous_B5_03_BL_T12.d Lipid_Pos_B2_03_BL_T12.d
SUBJECT_SAMPLE_FACTORS           	Subject_3	3_BL_16	Gender:F | Time:16 | Sleep Window (Hours):BL	RAW_FILE_NAME=Aqueous_B9_03_BL_T16B.d Lipid_Pos_B1_03_BL_T16.d
SUBJECT_SAMPLE_FACTORS           	Subject_3	3_BL_20	Gender:F | Time:20 | Sleep Window (Hours):BL	RAW_FILE_NAME=Aqueous_B9_03_BL_T20.d Lipid_Pos_B3_03_BL_T20.d
SUBJECT_SAMPLE_FACTORS           	Subject_3	3_BL_4	Gender:F | Time:4 | Sleep Window (Hours):BL	RAW_FILE_NAME=Aqueous_B8_03_BL_T4.d Lipid_Pos_B7_03_BL_T4_B.d
SUBJECT_SAMPLE_FACTORS           	Subject_3	3_BL_8	Gender:F | Time:8 | Sleep Window (Hours):BL	RAW_FILE_NAME=Aqueous_B3_03_BL_T8.d Lipid_Pos_B1_03_BL_T8.d
SUBJECT_SAMPLE_FACTORS           	Subject_510	510_5H_12	Gender:F | Time:12 | Sleep Window (Hours):5H	RAW_FILE_NAME=Aqueous_B3_0510_5H_T12.d Lipid_Pos_B5_0510_5H_T12.d
SUBJECT_SAMPLE_FACTORS           	Subject_510	510_5H_16	Gender:F | Time:16 | Sleep Window (Hours):5H	RAW_FILE_NAME=Aqueous_B6_0510_5H_T16.d Lipid_Pos_B1_0510_5H_T16.d
SUBJECT_SAMPLE_FACTORS           	Subject_510	510_5H_4	Gender:F | Time:4 | Sleep Window (Hours):5H	RAW_FILE_NAME=Aqueous_B8_0510_5H_T4.d Lipid_Pos_B4_0510_5H_T4.d
SUBJECT_SAMPLE_FACTORS           	Subject_510	510_5H_8	Gender:F | Time:8 | Sleep Window (Hours):5H	RAW_FILE_NAME=Aqueous_B5_0510_5H_T8.d Lipid_Pos_B2_0510_5H_T8.d
SUBJECT_SAMPLE_FACTORS           	Subject_510	510_9H_0	Gender:F | Time:zero | Sleep Window (Hours):9H	RAW_FILE_NAME=Aqueous_B8_0510_9H_T0.d Lipid_Pos_B5_0510_9H_T0.d
SUBJECT_SAMPLE_FACTORS           	Subject_510	510_9H_12	Gender:F | Time:12 | Sleep Window (Hours):9H	RAW_FILE_NAME=Aqueous_B9_0510_9H_T12.d Lipid_Pos_B7_0510_9H_T12.d
SUBJECT_SAMPLE_FACTORS           	Subject_510	510_9H_16	Gender:F | Time:16 | Sleep Window (Hours):9H	RAW_FILE_NAME=Aqueous_B8_0510_9H_T16.d Lipid_Pos_B5_0510_9H_T16.d
SUBJECT_SAMPLE_FACTORS           	Subject_510	510_9H_4	Gender:F | Time:4 | Sleep Window (Hours):9H	RAW_FILE_NAME=Aqueous_B3_0510_9H_T4.d Lipid_Pos_B5_0510_9H_T4.d
SUBJECT_SAMPLE_FACTORS           	Subject_510	510_9H_8	Gender:F | Time:8 | Sleep Window (Hours):9H	RAW_FILE_NAME=Aqueous_B9_0510_9H_T8.d Lipid_Pos_B7_0510_9H_T8_B.d
SUBJECT_SAMPLE_FACTORS           	Subject_7	7_5H_0	Gender:F | Time:zero | Sleep Window (Hours):5H	RAW_FILE_NAME=Aqueous_B5_07_5H_T0.d Lipid_Pos_B5_07_5H_T0.d
SUBJECT_SAMPLE_FACTORS           	Subject_7	7_5H_12	Gender:F | Time:12 | Sleep Window (Hours):5H	RAW_FILE_NAME=Aqueous_B7_07_5H_T12.d Lipid_Pos_B4_07_5H_T12.d
SUBJECT_SAMPLE_FACTORS           	Subject_7	7_5H_16	Gender:F | Time:16 | Sleep Window (Hours):5H	RAW_FILE_NAME=Aqueous_B4_07_5H_T16.d Lipid_Pos_B5_07_5H_T16_B.d
SUBJECT_SAMPLE_FACTORS           	Subject_7	7_5H_20	Gender:F | Time:20 | Sleep Window (Hours):5H	RAW_FILE_NAME=Aqueous_B6_07_5H_T20.d Lipid_Pos_B7_07_5H_T20.d
SUBJECT_SAMPLE_FACTORS           	Subject_7	7_5H_4	Gender:F | Time:4 | Sleep Window (Hours):5H	RAW_FILE_NAME=Aqueous_B4_07_5H_T4.d Lipid_Pos_B6_07_5H_T4.d
SUBJECT_SAMPLE_FACTORS           	Subject_7	7_5H_8	Gender:F | Time:8 | Sleep Window (Hours):5H	RAW_FILE_NAME=Aqueous_B4_07_5H_T8.d Lipid_Pos_B4_07_5H_T8.d
SUBJECT_SAMPLE_FACTORS           	Subject_7	7_9H_12	Gender:F | Time:12 | Sleep Window (Hours):9H	RAW_FILE_NAME=Aqueous_B8_07_9H_T12.d Lipid_Pos_B6_07_9H_T12.d
SUBJECT_SAMPLE_FACTORS           	Subject_7	7_9H_16	Gender:F | Time:16 | Sleep Window (Hours):9H	RAW_FILE_NAME=Aqueous_B4_07_9H_T16.d Lipid_Pos_B1_07_9H_T16.d
SUBJECT_SAMPLE_FACTORS           	Subject_7	7_9H_20	Gender:F | Time:20 | Sleep Window (Hours):9H	RAW_FILE_NAME=Aqueous_B5_07_9H_T20.d Lipid_Pos_B5_07_9H_T20.d
SUBJECT_SAMPLE_FACTORS           	Subject_7	7_9H_4	Gender:F | Time:4 | Sleep Window (Hours):9H	RAW_FILE_NAME=Aqueous_B7_07_9H_T4.d Lipid_Pos_B3_07_9H_T4.d
SUBJECT_SAMPLE_FACTORS           	Subject_7	7_9H_8	Gender:F | Time:8 | Sleep Window (Hours):9H	RAW_FILE_NAME=Aqueous_B4_07_9H_T8.d Lipid_Pos_B2_07_9H_T8.d
#COLLECTION
CO:COLLECTION_SUMMARY            	These blood (plasma) samples were collected through an IV catheter in human
CO:COLLECTION_SUMMARY            	participants in a Clinical Translational Study investigating the metabolic
CO:COLLECTION_SUMMARY            	consequences of insufficient sleep.
CO:SAMPLE_TYPE                   	Blood (plasma)
#TREATMENT
TR:TREATMENT_SUMMARY             	This was a cross-over design with baseline (BL; 9h per night sleep with
TR:TREATMENT_SUMMARY             	controlled energy intake), control (9H; 9h per night sleep with ad libitum
TR:TREATMENT_SUMMARY             	energy intake), and insufficient sleep (5H; 5h per night sleep with ad libitum
TR:TREATMENT_SUMMARY             	energy intake) conditions. Baseline lasted 3 days and control and insufficient
TR:TREATMENT_SUMMARY             	sleep conditions lasted 5 days.
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	For sample preparation, plasma samples were thawed at room temperature and
SP:SAMPLEPREP_SUMMARY            	briefly vortexed. 100 uL of each sample was transferred to a 1.5 mL
SP:SAMPLEPREP_SUMMARY            	microcentrifuge tube and kept at 0°C. 10 uL of hydrophobic and hydrophilic
SP:SAMPLEPREP_SUMMARY            	standards and spikes at room temperature were added to each sample, and then
SP:SAMPLEPREP_SUMMARY            	each sample vortexed for 10s. 400 uL of ice-cold methanol was added to
SP:SAMPLEPREP_SUMMARY            	precipitate proteins. Tubes were then vortexed for 10s and then centrifuged for
SP:SAMPLEPREP_SUMMARY            	15 min at 0°C at 18,000 x g. The supernatant was transferred to a clean glass
SP:SAMPLEPREP_SUMMARY            	culture tube using a plastic pipette. Samples were dried in glass culture tubes
SP:SAMPLEPREP_SUMMARY            	placed under N2 at 35°C for approximately 1h. Using a glass pipette, 3 mL of
SP:SAMPLEPREP_SUMMARY            	methyl tert-butyl ether (MTBE) was added to the dried methanol residue in each
SP:SAMPLEPREP_SUMMARY            	glass culture tube. Tubes were vortexed for 30s. 750 uL of water was added and
SP:SAMPLEPREP_SUMMARY            	tubes were vortexed for 10s. Tubes were centrifuged for 10 min at room
SP:SAMPLEPREP_SUMMARY            	temperature at ~200 x g. 2.5 mL of the resulting MTBE layer (hydrophobic
SP:SAMPLEPREP_SUMMARY            	fraction) was transferred to a new glass culture tube; the remaining layer was
SP:SAMPLEPREP_SUMMARY            	the hydrophilic fraction. 3.0 mL of MTBE was added to the remaining hydrophilic
SP:SAMPLEPREP_SUMMARY            	fraction and tubes were vortexed for 10s. These tubes were again centrifuged for
SP:SAMPLEPREP_SUMMARY            	10 min at room temperature at ~200 x g. 3.0 mL of MTBE was aspirated and
SP:SAMPLEPREP_SUMMARY            	combined with the first MTBE layer. The MTBE fractions were dried under N2 at
SP:SAMPLEPREP_SUMMARY            	35°C and immediately re-suspended in 200 uL of methanol. Each tube was vortexed
SP:SAMPLEPREP_SUMMARY            	for 5s, transferred to a glass auto-sampler vial with a glass insert using a
SP:SAMPLEPREP_SUMMARY            	Pasteur pipette. Samples were stored at -80°C. The hydrophilic fractions were
SP:SAMPLEPREP_SUMMARY            	dried under N2 at 35°C. 100 uL of water and 400 uL of ice-cold methanol were
SP:SAMPLEPREP_SUMMARY            	added to the dried hydrophilic fraction. Tubes were vortexed for 10s and
SP:SAMPLEPREP_SUMMARY            	centrifuged immediately at ~200 x g for 1 min. Supernatants from each tube were
SP:SAMPLEPREP_SUMMARY            	transferred to a 1.5 mL microcentrifuge tube using a Pasteur pipette. These
SP:SAMPLEPREP_SUMMARY            	tubes were then stored at -80°C for 25 min then centrifuged for 15 min at 0°C
SP:SAMPLEPREP_SUMMARY            	and 18,000 x g. The supernatant was transferred to a new 1.5 mL microcentrifuge
SP:SAMPLEPREP_SUMMARY            	tube using a plastic pipette. These tubes were dried in a vacuum centrifugal
SP:SAMPLEPREP_SUMMARY            	concentrator at 45°C and re-suspended in 100 uL of 95:5 water: acetonitrile.
SP:SAMPLEPREP_SUMMARY            	Each tube was vortexed for 30s and transferred to a glass auto-sampler vial with
SP:SAMPLEPREP_SUMMARY            	a glass insert using a plastic pipette tip. Samples were stored at -80°C.
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_TYPE           	Reversed phase
CH:INSTRUMENT_NAME               	Agilent 1290
CH:COLUMN_NAME                   	Agilent Zorbax RRHD SB-C18 (100 x 2.1mm, 1.8um)
#ANALYSIS
AN:ANALYSIS_TYPE                 	MS
#MS
MS:INSTRUMENT_NAME               	Agilent 6220 TOF
MS:INSTRUMENT_TYPE               	QTOF
MS:MS_TYPE                       	ESI
MS:ION_MODE                      	POSITIVE
MS:MS_COMMENTS                   	The hydrophobic fraction MS conditions were as follows: Agilent 6220
MS:MS_COMMENTS                   	Time-of-Flight (TOF)-MS with dual ESI source, scan rate 2.02 spectra/s, mass
MS:MS_COMMENTS                   	range 60–1600 m/z, gas temperature 300°C, gas flow 12.0 L/min, nebulizer 30
MS:MS_COMMENTS                   	psi, skimmer 60V, capillary voltage 4000V, fragmentor 120V, reference masses
MS:MS_COMMENTS                   	121.050873 and 922.009798 (Agilent reference mix).
MS:MS_RESULTS_FILE               	ST001440_AN002405_Results.txt	UNITS:raw counts	Has m/z:Yes	Has RT:Yes	RT units:Minutes
#END