#METABOLOMICS WORKBENCH cclish_20201105_092933_mwtab.txt DATATRACK_ID:2230 STUDY_ID:ST001522 ANALYSIS_ID:AN002540 PROJECT_ID:000000
VERSION             	1
CREATED_ON             	November 5, 2020, 2:22 pm
#PROJECT
PR:PROJECT_TITLE                 	Role of Diet in the Reconstitution of the Human Gut Microbiome and its
PR:PROJECT_TITLE                 	Metabolome
PR:PROJECT_TYPE                  	Observational study
PR:PROJECT_SUMMARY               	We studied the impact of three divergent diets, vegan, omnivore, and a synthetic
PR:PROJECT_SUMMARY               	enteral nutrition (EEN) diet lacking fiber, on the human gut microbiome and its
PR:PROJECT_SUMMARY               	metabolome in a longitudinal analysis that included a microbiota depletion
PR:PROJECT_SUMMARY               	intervention. Hybrid nontargeted LC-MS methods were used to profile stool and
PR:PROJECT_SUMMARY               	plasma metabolites.
PR:INSTITUTE                     	Broad Institute of MIT and Harvard
PR:LAST_NAME                     	Clish
PR:FIRST_NAME                    	Clary
PR:ADDRESS                       	415 Main Street, Cambridge, MA, 02142, USA
PR:EMAIL                         	clary@broadinstitute.org
PR:PHONE                         	617-714-7654
PR:FUNDING_SOURCE                	Crohn’s & Colitis Foundation, P30 DK 050306, PennCHOP Microbiome Program, and
PR:FUNDING_SOURCE                	the Penn Center for Nutritional Science and Medicine
PR:CONTRIBUTORS                  	Ceylan Tanes, Kyle Bittinger, Yuan Gao, Elliot S. Friedman, Lisa Nessel, Unmesha
PR:CONTRIBUTORS                  	Roy Paladhi, Lillian Chau, Erika Panfen, Michael A. Fischbach, Jonathan Braun,
PR:CONTRIBUTORS                  	Ramnik J. Xavier, Clary B. Clish, Hongzhe Li, Frederic D. Bushman, James D.
PR:CONTRIBUTORS                  	Lewis, Gary D. Wu
#STUDY
ST:STUDY_TITLE                   	Plasma unknowns profiled using hybrid nontargeted methods (part-IV)
ST:STUDY_SUMMARY                 	We determined the effect of diet on the composition and metabolic function of
ST:STUDY_SUMMARY                 	human gut microbiome using a controlled feeding experiment with three divergent
ST:STUDY_SUMMARY                 	diets (vegan, omnivore, and an exclusive enteral nutrition diet (EEN) devoid of
ST:STUDY_SUMMARY                 	dietary fiber) in the Food And Resulting Microbial Metabolites (FARMM) study.
ST:STUDY_SUMMARY                 	The study included an antibiotic and polyethylene glycol (Abx/PEG) intervention
ST:STUDY_SUMMARY                 	to dynamically assess the effect of diet on the reconstitution of the gut
ST:STUDY_SUMMARY                 	microbiota and its associated fecal and plasma metabolome. Samples from thirty
ST:STUDY_SUMMARY                 	healthy volunteers between the ages of 18 and 60, 10 mper diet group were
ST:STUDY_SUMMARY                 	analyzed. Self-reported vegans were required to have followed a vegan diet for a
ST:STUDY_SUMMARY                 	minimum of 6 months prior to enrollment. Key exclusion criteria included
ST:STUDY_SUMMARY                 	inflammatory bowel disease, celiac disease, or other chronic intestinal
ST:STUDY_SUMMARY                 	disorders; prior bowel resection surgery other than appendectomy; baseline bowel
ST:STUDY_SUMMARY                 	frequency less than every 2 days or greater than 3 times daily; creatinine
ST:STUDY_SUMMARY                 	concentration greater than the upper limit of normal; diabetes mellitus;
ST:STUDY_SUMMARY                 	currently smoking; body mass index (BMI) <18.5 or >35; and use of antibiotics
ST:STUDY_SUMMARY                 	and probiotics in the prior 6 months. The 10 vegans continued to follow their
ST:STUDY_SUMMARY                 	usual diet as outpatients. All participants completed the Diet History
ST:STUDY_SUMMARY                 	Questionnaire II (DHQ II), a food frequency questionnaire developed by the Risk
ST:STUDY_SUMMARY                 	Factor Monitoring and Methods Branch of the National Cancer Institute. The vegan
ST:STUDY_SUMMARY                 	participants also completed three 24 hour diet recalls with a dietitian in the
ST:STUDY_SUMMARY                 	week prior to starting antibiotics. We randomly assigned the 20 omnivores to
ST:STUDY_SUMMARY                 	receive an omnivore diet or EEN (Modulen® IBD) while residing in an inpatient
ST:STUDY_SUMMARY                 	research unit. The macronutrient composition of Modulen® is protein 36g, fat
ST:STUDY_SUMMARY                 	47g, and carbohydrate 110g per 1000 Kcal. The two omnivore diets were engineered
ST:STUDY_SUMMARY                 	to have a similar composition to EEN. All the subjects consumed the menu A on
ST:STUDY_SUMMARY                 	day 11 and menu B on days 4 and 14. Diets for omnivores were constructed to
ST:STUDY_SUMMARY                 	provide the expected total calories required per day for the participant to
ST:STUDY_SUMMARY                 	maintain their current weight and were adjusted if there was weight gain or loss
ST:STUDY_SUMMARY                 	of more than 2.5 pounds. On days 6, 7, and 8, inpatients participants received
ST:STUDY_SUMMARY                 	vancomycin 500mg orally every 6 hours and neomycin 1000mg orally every 6 hours.
ST:STUDY_SUMMARY                 	On day 7, participants consumed 4L of polyethylene glycol (PEG) based bowel
ST:STUDY_SUMMARY                 	purgative (GoLytely®). Participants in the omnivore and EEN arms left the
ST:STUDY_SUMMARY                 	inpatient research unit only under the direct supervision of a research staff
ST:STUDY_SUMMARY                 	member. The vegan outpatients reported to the hospital twice on days 6,7 and 8
ST:STUDY_SUMMARY                 	to receive antibiotics and to consume PEG on day 7. The first stool sample of
ST:STUDY_SUMMARY                 	each day of the inpatient groups was collected, aliquoted and frozen immediately
ST:STUDY_SUMMARY                 	at -80oC. Blood was collected on days 1, 5, 9, 12 and 15 from which plasma
ST:STUDY_SUMMARY                 	aliquots were immediately isolated and frozen at -80oC. The outpatient
ST:STUDY_SUMMARY                 	participants following the vegan diet also had blood collected on days 1, 5, 9,
ST:STUDY_SUMMARY                 	12, and 15 from which plasma aliquots were immediately isolated and frozen at
ST:STUDY_SUMMARY                 	-80oC. Among these participants, the first stool of the day was collected at
ST:STUDY_SUMMARY                 	home daily and kept on ice packs until it was brought to the research unit where
ST:STUDY_SUMMARY                 	it could be aliquoted and frozen. Samples from vegans were received for
ST:STUDY_SUMMARY                 	aliquoting within 24 hours and on average within 4 hours (Wu et al., 2010). Day
ST:STUDY_SUMMARY                 	0 stool was not collected from vegans since their diet did not change. The
ST:STUDY_SUMMARY                 	aliquoted amounts for all samples ranged from 500 mg to 1 g. The aliquots were
ST:STUDY_SUMMARY                 	taken from different areas of the sample. Remaining sample was then collected
ST:STUDY_SUMMARY                 	into one residual 50 ml conical tube with a tongue depressor. Any remaining
ST:STUDY_SUMMARY                 	stool was discarded. The University of Pennsylvania Institutional Review Board
ST:STUDY_SUMMARY                 	(IRB) approved the research protocol.
ST:INSTITUTE                     	Broad Institute of MIT and Harvard
ST:LAST_NAME                     	Clish
ST:FIRST_NAME                    	Clary
ST:ADDRESS                       	415 Main Street, Cambridge, MA, 02142, USA
ST:EMAIL                         	clary@broadinstitute.org
ST:PHONE                         	617-714-7654
ST:NUM_GROUPS                    	3
ST:TOTAL_SUBJECTS                	30
ST:NUM_MALES                     	20
ST:NUM_FEMALES                   	10
ST:STUDY_COMMENTS                	plasma samples collected at baseline and 3-4 timepoints
#SUBJECT
SU:SUBJECT_TYPE                  	Human
SU:SUBJECT_SPECIES               	Homo sapiens
SU:TAXONOMY_ID                   	9606
SU:AGE_OR_AGE_RANGE              	20-60
SU:WEIGHT_OR_WEIGHT_RANGE        	BMI range: 20-35
SU:GENDER                        	Male and female
SU:HUMAN_RACE                    	White; American Indian/Alaskan Native; Black/African American
SU:HUMAN_ETHNICITY               	Hispanic or Latino; Non-Hispanic or Latino
SU:HUMAN_TRIAL_TYPE              	Intervention trial
SU:HUMAN_EXCLUSION_CRITERIA      	Key exclusion criteria included inflammatory bowel disease, celiac disease, or
SU:HUMAN_EXCLUSION_CRITERIA      	other chronic intestinal disorders; prior bowel resection surgery other than
SU:HUMAN_EXCLUSION_CRITERIA      	appendectomy; baseline bowel frequency less than every 2 days or greater than 3
SU:HUMAN_EXCLUSION_CRITERIA      	times daily; creatinine concentration greater than the upper limit of normal;
SU:HUMAN_EXCLUSION_CRITERIA      	diabetes mellitus; currently smoking; body mass index (BMI) <18.5 or >35; and
SU:HUMAN_EXCLUSION_CRITERIA      	use of antibiotics and probiotics in the prior 6 months
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data
SUBJECT_SAMPLE_FACTORS           	9002	9002-3-PA	Study_Diet:Vegan	Type=Plasma; Age=20; Sex=Male; BMI=21.3; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 15; RAW_FILE_NAME=0078_WUG_FARMM_HIL-9002-3-PA.raw 0078_WUG_FARMM_LIP-9002-3-PA.raw 0078_WU_FARMM_CMH_9002-3-PA.raw 0078_WUG_FARMM_FFA-9002-3-PA.raw
SUBJECT_SAMPLE_FACTORS           	9002	9002-3-PB	Study_Diet:Vegan	Type=Plasma; Age=20; Sex=Male; BMI=21.3; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 12; RAW_FILE_NAME=0076_WUG_FARMM_HIL-9002-3-PB.raw 0076_WUG_FARMM_LIP-9002-3-PB.raw 0076_WU_FARMM_CMH_9002-3-PB.raw 0076_WUG_FARMM_FFA-9002-3-PB.raw
SUBJECT_SAMPLE_FACTORS           	9002	9002-3-PC	Study_Diet:Vegan	Type=Plasma; Age=20; Sex=Male; BMI=21.3; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 9; RAW_FILE_NAME=0079_WUG_FARMM_HIL-9002-3-PC.raw 0079_WUG_FARMM_LIP-9002-3-PC.raw 0079_WU_FARMM_CMH_9002-3-PC.raw 0079_WUG_FARMM_FFA-9002-3-PC.raw
SUBJECT_SAMPLE_FACTORS           	9002	9002-3-PD	Study_Diet:Vegan	Type=Plasma; Age=20; Sex=Male; BMI=21.3; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 5; RAW_FILE_NAME=0080_WUG_FARMM_HIL-9002-3-PD.raw 0080_WUG_FARMM_LIP-9002-3-PD.raw 0080_WU_FARMM_CMH_9002-3-PD.raw 0080_WUG_FARMM_FFA-9002-3-PD.raw
SUBJECT_SAMPLE_FACTORS           	9002	9002-3-PE	Study_Diet:Vegan	Type=Plasma; Age=20; Sex=Male; BMI=21.3; Ethnicity=Not Hispanic or Latino; Race=White; Time=Baseline; RAW_FILE_NAME=0077_WUG_FARMM_HIL-9002-3-PE.raw 0077_WUG_FARMM_LIP-9002-3-PE.raw 0077_WU_FARMM_CMH_9002-3-PE.raw 0077_WUG_FARMM_FFA-9002-3-PE.raw
SUBJECT_SAMPLE_FACTORS           	9003	9003-2-PA	Study_Diet:Modulen	Type=Plasma; Age=20; Sex=Male; BMI=20.3; Ethnicity=NA; Race=White; Time=Day 15; RAW_FILE_NAME=0144_WUG_FARMM_HIL-9003-2-PA.raw 0144_WUG_FARMM_LIP-9003-2-PA.raw 0144_WU_FARMM_CMH_9003-2-PA.raw 0144_WUG_FARMM_FFA-9003-2-PA.raw
SUBJECT_SAMPLE_FACTORS           	9003	9003-2-PB	Study_Diet:Modulen	Type=Plasma; Age=20; Sex=Male; BMI=20.3; Ethnicity=NA; Race=White; Time=Day 12; RAW_FILE_NAME=0141_WUG_FARMM_HIL-9003-2-PB.raw 0141_WUG_FARMM_LIP-9003-2-PB.raw 0141_WU_FARMM_CMH_9003-2-PB.raw 0141_WUG_FARMM_FFA-9003-2-PB.raw
SUBJECT_SAMPLE_FACTORS           	9003	9003-2-PC	Study_Diet:Modulen	Type=Plasma; Age=20; Sex=Male; BMI=20.3; Ethnicity=NA; Race=White; Time=Day 9; RAW_FILE_NAME=0143_WUG_FARMM_HIL-9003-2-PC.raw 0143_WUG_FARMM_LIP-9003-2-PC.raw 0143_WU_FARMM_CMH_9003-2-PC.raw 0143_WUG_FARMM_FFA-9003-2-PC.raw
SUBJECT_SAMPLE_FACTORS           	9003	9003-2-PD	Study_Diet:Modulen	Type=Plasma; Age=20; Sex=Male; BMI=20.3; Ethnicity=NA; Race=White; Time=Day 5; RAW_FILE_NAME=0142_WUG_FARMM_HIL-9003-2-PD.raw 0142_WUG_FARMM_LIP-9003-2-PD.raw 0142_WU_FARMM_CMH_9003-2-PD.raw 0142_WUG_FARMM_FFA-9003-2-PD.raw
SUBJECT_SAMPLE_FACTORS           	9003	9003-2-PE	Study_Diet:Modulen	Type=Plasma; Age=20; Sex=Male; BMI=20.3; Ethnicity=NA; Race=White; Time=Baseline; RAW_FILE_NAME=0145_WUG_FARMM_HIL-9003-2-PE.raw 0145_WUG_FARMM_LIP-9003-2-PE.raw 0145_WU_FARMM_CMH_9003-2-PE.raw 0145_WUG_FARMM_FFA-9003-2-PE.raw
SUBJECT_SAMPLE_FACTORS           	9004	9004-3-PA	Study_Diet:Vegan	Type=Plasma; Age=24; Sex=Male; BMI=21.7; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 15; RAW_FILE_NAME=0091_WUG_FARMM_HIL-9004-3-PA.raw 0091_WUG_FARMM_LIP-9004-3-PA.raw 0091_WU_FARMM_CMH_9004-3-PA.raw 0091_WUG_FARMM_FFA-9004-3-PA.raw
SUBJECT_SAMPLE_FACTORS           	9004	9004-3-PB	Study_Diet:Vegan	Type=Plasma; Age=24; Sex=Male; BMI=21.7; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 12; RAW_FILE_NAME=0092_WUG_FARMM_HIL-9004-3-PB.raw 0092_WUG_FARMM_LIP-9004-3-PB.raw 0092_WU_FARMM_CMH_9004-3-PB.raw 0092_WUG_FARMM_FFA-9004-3-PB.raw
SUBJECT_SAMPLE_FACTORS           	9004	9004-3-PC	Study_Diet:Vegan	Type=Plasma; Age=24; Sex=Male; BMI=21.7; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 9; RAW_FILE_NAME=0095_WUG_FARMM_HIL-9004-3-PC.raw 0095_WUG_FARMM_LIP-9004-3-PC.raw 0095_WU_FARMM_CMH_9004-3-PC.raw 0095_WUG_FARMM_FFA-9004-3-PC.raw
SUBJECT_SAMPLE_FACTORS           	9004	9004-3-PD	Study_Diet:Vegan	Type=Plasma; Age=24; Sex=Male; BMI=21.7; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 5; RAW_FILE_NAME=0093_WUG_FARMM_HIL-9004-3-PD.raw 0093_WUG_FARMM_LIP-9004-3-PD.raw 0093_WU_FARMM_CMH_9004-3-PD.raw 0093_WUG_FARMM_FFA-9004-3-PD.raw
SUBJECT_SAMPLE_FACTORS           	9004	9004-3-PE	Study_Diet:Vegan	Type=Plasma; Age=24; Sex=Male; BMI=21.7; Ethnicity=Not Hispanic or Latino; Race=White; Time=Baseline; RAW_FILE_NAME=0094_WUG_FARMM_HIL-9004-3-PE.raw 0094_WUG_FARMM_LIP-9004-3-PE.raw 0094_WU_FARMM_CMH_9004-3-PE.raw 0094_WUG_FARMM_FFA-9004-3-PE.raw
SUBJECT_SAMPLE_FACTORS           	9005	9005-1-PA	Study_Diet:Western	Type=Plasma; Age=23; Sex=Male; BMI=21.6; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 15; RAW_FILE_NAME=0109_WUG_FARMM_HIL-9005-1-PA.raw 0150d_0109ri_WUG_FARMM_LIP-9005-1-PA.raw 0109_WU_FARMM_CMH_9005-1-PA.raw 0109_WUG_FARMM_FFA-9005-1-PA.raw
SUBJECT_SAMPLE_FACTORS           	9005	9005-1-PB	Study_Diet:Western	Type=Plasma; Age=23; Sex=Male; BMI=21.6; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 12; RAW_FILE_NAME=0106_WUG_FARMM_HIL-9005-1-PB.raw 0106_WUG_FARMM_LIP-9005-1-PB.raw 0106_WU_FARMM_CMH_9005-1-PB.raw 0106_WUG_FARMM_FFA-9005-1-PB.raw
SUBJECT_SAMPLE_FACTORS           	9005	9005-1-PC	Study_Diet:Western	Type=Plasma; Age=23; Sex=Male; BMI=21.6; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 9; RAW_FILE_NAME=0108_WUG_FARMM_HIL-9005-1-PC.raw 0150c_0108ri_WUG_FARMM_LIP-9005-1-PC.raw 0108_WU_FARMM_CMH_9005-1-PC.raw 0108_WUG_FARMM_FFA-9005-1-PC.raw
SUBJECT_SAMPLE_FACTORS           	9005	9005-1-PD	Study_Diet:Western	Type=Plasma; Age=23; Sex=Male; BMI=21.6; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 5; RAW_FILE_NAME=0107_WUG_FARMM_HIL-9005-1-PD.raw 0107_WUG_FARMM_LIP-9005-1-PD.raw 0107_WU_FARMM_CMH_9005-1-PD.raw 0107_WUG_FARMM_FFA-9005-1-PD.raw
SUBJECT_SAMPLE_FACTORS           	9005	9005-1-PE	Study_Diet:Western	Type=Plasma; Age=23; Sex=Male; BMI=21.6; Ethnicity=Not Hispanic or Latino; Race=White; Time=Baseline; RAW_FILE_NAME=0110_WUG_FARMM_HIL-9005-1-PE.raw 0150e_0110ri_WUG_FARMM_LIP-9005-1-PE.raw 0110_WU_FARMM_CMH_9005-1-PE.raw 0110_WUG_FARMM_FFA-9005-1-PE.raw
SUBJECT_SAMPLE_FACTORS           	9006	9006-1-PA	Study_Diet:Western	Type=Plasma; Age=24; Sex=Male; BMI=28.2; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 15; RAW_FILE_NAME=0047_WUG_FARMM_HIL-9006-1-PA.raw 0047_WUG_FARMM_LIP-9006-1-PA.raw 0047_WU_FARMM_CMH_9006-1-PA.raw 0047_WUG_FARMM_FFA-9006-1-PA.raw
SUBJECT_SAMPLE_FACTORS           	9006	9006-1-PB	Study_Diet:Western	Type=Plasma; Age=24; Sex=Male; BMI=28.2; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 12; RAW_FILE_NAME=0048_WUG_FARMM_HIL-9006-1-PB.raw 0048_WUG_FARMM_LIP-9006-1-PB.raw 0048_WU_FARMM_CMH_9006-1-PB.raw 0048_WUG_FARMM_FFA-9006-1-PB.raw
SUBJECT_SAMPLE_FACTORS           	9006	9006-1-PC	Study_Diet:Western	Type=Plasma; Age=24; Sex=Male; BMI=28.2; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 9; RAW_FILE_NAME=0050_WUG_FARMM_HIL-9006-1-PC.raw 0050_WUG_FARMM_LIP-9006-1-PC.raw 0050_WU_FARMM_CMH_9006-1-PC.raw 0050_WUG_FARMM_FFA-9006-1-PC.raw
SUBJECT_SAMPLE_FACTORS           	9006	9006-1-PD	Study_Diet:Western	Type=Plasma; Age=24; Sex=Male; BMI=28.2; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 5; RAW_FILE_NAME=0046_WUG_FARMM_HIL-9006-1-PD.raw 0046_WUG_FARMM_LIP-9006-1-PD.raw 0046_WU_FARMM_CMH_9006-1-PD.raw 0046_WUG_FARMM_FFA-9006-1-PD.raw
SUBJECT_SAMPLE_FACTORS           	9006	9006-1-PE	Study_Diet:Western	Type=Plasma; Age=24; Sex=Male; BMI=28.2; Ethnicity=Not Hispanic or Latino; Race=White; Time=Baseline; RAW_FILE_NAME=0049_WUG_FARMM_HIL-9006-1-PE.raw 0049_WUG_FARMM_LIP-9006-1-PE.raw 0049_WU_FARMM_CMH_9006-1-PE.raw 0049_WUG_FARMM_FFA-9006-1-PE.raw
SUBJECT_SAMPLE_FACTORS           	9008	9008-3-PA	Study_Diet:Vegan	Type=Plasma; Age=23; Sex=Male; BMI=20; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 15; RAW_FILE_NAME=0103_WUG_FARMM_HIL-9008-3-PA.raw 0103_WUG_FARMM_LIP-9008-3-PA.raw 0103_WU_FARMM_CMH_9008-3-PA.raw 0103_WUG_FARMM_FFA-9008-3-PA.raw
SUBJECT_SAMPLE_FACTORS           	9008	9008-3-PB	Study_Diet:Vegan	Type=Plasma; Age=23; Sex=Male; BMI=20; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 12; RAW_FILE_NAME=0105_WUG_FARMM_HIL-9008-3-PB.raw 0105_WUG_FARMM_LIP-9008-3-PB.raw 0105_WU_FARMM_CMH_9008-3-PB.raw 0105_WUG_FARMM_FFA-9008-3-PB.raw
SUBJECT_SAMPLE_FACTORS           	9008	9008-3-PC	Study_Diet:Vegan	Type=Plasma; Age=23; Sex=Male; BMI=20; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 9; RAW_FILE_NAME=0101_WUG_FARMM_HIL-9008-3-PC.raw 0101_WUG_FARMM_LIP-9008-3-PC.raw 0101_WU_FARMM_CMH_9008-3-PC.raw 0101_WUG_FARMM_FFA-9008-3-PC.raw
SUBJECT_SAMPLE_FACTORS           	9008	9008-3-PD	Study_Diet:Vegan	Type=Plasma; Age=23; Sex=Male; BMI=20; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 5; RAW_FILE_NAME=0104_WUG_FARMM_HIL-9008-3-PD.raw 0104_WUG_FARMM_LIP-9008-3-PD.raw 0104_WU_FARMM_CMH_9008-3-PD.raw 0104_WUG_FARMM_FFA-9008-3-PD.raw
SUBJECT_SAMPLE_FACTORS           	9008	9008-3-PE	Study_Diet:Vegan	Type=Plasma; Age=23; Sex=Male; BMI=20; Ethnicity=Not Hispanic or Latino; Race=White; Time=Baseline; RAW_FILE_NAME=0102_WUG_FARMM_HIL-9008-3-PE.raw 0102_WUG_FARMM_LIP-9008-3-PE.raw 0102_WU_FARMM_CMH_9008-3-PE.raw 0102_WUG_FARMM_FFA-9008-3-PE.raw
SUBJECT_SAMPLE_FACTORS           	9009	9009-2-PA	Study_Diet:Modulen	Type=Plasma; Age=23; Sex=Male; BMI=22.4; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 15; RAW_FILE_NAME=0131_WUG_FARMM_HIL-9009-2-PA.raw 0131_WUG_FARMM_LIP-9009-2-PA.raw 0131_WU_FARMM_CMH_9009-2-PA.raw 0131_WUG_FARMM_FFA-9009-2-PA.raw
SUBJECT_SAMPLE_FACTORS           	9009	9009-2-PB	Study_Diet:Modulen	Type=Plasma; Age=23; Sex=Male; BMI=22.4; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 12; RAW_FILE_NAME=0132_WUG_FARMM_HIL-9009-2-PB.raw 0132_WUG_FARMM_LIP-9009-2-PB.raw 0132_WU_FARMM_CMH_9009-2-PB.raw 0132_WUG_FARMM_FFA-9009-2-PB.raw
SUBJECT_SAMPLE_FACTORS           	9009	9009-2-PC	Study_Diet:Modulen	Type=Plasma; Age=23; Sex=Male; BMI=22.4; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 9; RAW_FILE_NAME=0133_WUG_FARMM_HIL-9009-2-PC.raw 0133_WUG_FARMM_LIP-9009-2-PC.raw 0133_WU_FARMM_CMH_9009-2-PC.raw 0133_WUG_FARMM_FFA-9009-2-PC.raw
SUBJECT_SAMPLE_FACTORS           	9009	9009-2-PD	Study_Diet:Modulen	Type=Plasma; Age=23; Sex=Male; BMI=22.4; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 5; RAW_FILE_NAME=0134_WUG_FARMM_HIL-9009-2-PD.raw 0134_WUG_FARMM_LIP-9009-2-PD.raw 0134_WU_FARMM_CMH_9009-2-PD.raw 0134_WUG_FARMM_FFA-9009-2-PD.raw
SUBJECT_SAMPLE_FACTORS           	9009	9009-2-PE	Study_Diet:Modulen	Type=Plasma; Age=23; Sex=Male; BMI=22.4; Ethnicity=Not Hispanic or Latino; Race=White; Time=Baseline; RAW_FILE_NAME=0135_WUG_FARMM_HIL-9009-2-PE.raw 0135_WUG_FARMM_LIP-9009-2-PE.raw 0135_WU_FARMM_CMH_9009-2-PE.raw 0135_WUG_FARMM_FFA-9009-2-PE.raw
SUBJECT_SAMPLE_FACTORS           	9010	9010-1-PA	Study_Diet:Western	Type=Plasma; Age=24; Sex=Female; BMI=21.8; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 15; RAW_FILE_NAME=0149_WUG_FARMM_HIL-9010-1-PA.raw 0149_WUG_FARMM_LIP-9010-1-PA.raw 0149_WU_FARMM_CMH_9010-1-PA.raw 0149_WUG_FARMM_FFA-9010-1-PA.raw
SUBJECT_SAMPLE_FACTORS           	9010	9010-1-PB	Study_Diet:Western	Type=Plasma; Age=24; Sex=Female; BMI=21.8; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 12; RAW_FILE_NAME=0148_WUG_FARMM_HIL-9010-1-PB.raw 0148_WUG_FARMM_LIP-9010-1-PB.raw 0148_WU_FARMM_CMH_9010-1-PB.raw 0148_WUG_FARMM_FFA-9010-1-PB.raw
SUBJECT_SAMPLE_FACTORS           	9010	9010-1-PC	Study_Diet:Western	Type=Plasma; Age=24; Sex=Female; BMI=21.8; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 9; RAW_FILE_NAME=0147_WUG_FARMM_HIL-9010-1-PC.raw 0147_WUG_FARMM_LIP-9010-1-PC.raw 0147_WU_FARMM_CMH_9010-1-PC.raw 0147_WUG_FARMM_FFA-9010-1-PC.raw
SUBJECT_SAMPLE_FACTORS           	9010	9010-1-PD	Study_Diet:Western	Type=Plasma; Age=24; Sex=Female; BMI=21.8; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 5; RAW_FILE_NAME=0146_WUG_FARMM_HIL-9010-1-PD.raw 0146_WUG_FARMM_LIP-9010-1-PD.raw 0146_WU_FARMM_CMH_9010-1-PD.raw 0146_WUG_FARMM_FFA-9010-1-PD.raw
SUBJECT_SAMPLE_FACTORS           	9010	9010-1-PE	Study_Diet:Western	Type=Plasma; Age=24; Sex=Female; BMI=21.8; Ethnicity=Not Hispanic or Latino; Race=White; Time=Baseline; RAW_FILE_NAME=0150_WUG_FARMM_HIL-9010-1-PE.raw 0150_WUG_FARMM_LIP-9010-1-PE.raw 0150_WU_FARMM_CMH_9010-1-PE.raw 0150_WUG_FARMM_FFA-9010-1-PE.raw
SUBJECT_SAMPLE_FACTORS           	9013	9013-2-PA	Study_Diet:Modulen	Type=Plasma; Age=40; Sex=Male; BMI=24.1; Ethnicity=Hispanic or Latino; Race=White; Time=Day 15; RAW_FILE_NAME=0075_WUG_FARMM_HIL-9013-2-PA.raw 0075_WUG_FARMM_LIP-9013-2-PA.raw 0075_WU_FARMM_CMH_9013-2-PA.raw 0075_WUG_FARMM_FFA-9013-2-PA.raw
SUBJECT_SAMPLE_FACTORS           	9013	9013-2-PB	Study_Diet:Modulen	Type=Plasma; Age=40; Sex=Male; BMI=24.1; Ethnicity=Hispanic or Latino; Race=White; Time=Day 12; RAW_FILE_NAME=0074_WUG_FARMM_HIL-9013-2-PB.raw 0074_WUG_FARMM_LIP-9013-2-PB.raw 0074_WU_FARMM_CMH_9013-2-PB.raw 0074_WUG_FARMM_FFA-9013-2-PB.raw
SUBJECT_SAMPLE_FACTORS           	9013	9013-2-PC	Study_Diet:Modulen	Type=Plasma; Age=40; Sex=Male; BMI=24.1; Ethnicity=Hispanic or Latino; Race=White; Time=Day 9; RAW_FILE_NAME=0072_WUG_FARMM_HIL-9013-2-PC.raw 0072_WUG_FARMM_LIP-9013-2-PC.raw 0072_WU_FARMM_CMH_9013-2-PC.raw 0072_WUG_FARMM_FFA-9013-2-PC.raw
SUBJECT_SAMPLE_FACTORS           	9013	9013-2-PD	Study_Diet:Modulen	Type=Plasma; Age=40; Sex=Male; BMI=24.1; Ethnicity=Hispanic or Latino; Race=White; Time=Day 5; RAW_FILE_NAME=0071_WUG_FARMM_HIL-9013-2-PD.raw 0071_WUG_FARMM_LIP-9013-2-PD.raw 0071_WU_FARMM_CMH_9013-2-PD.raw 0071_WUG_FARMM_FFA-9013-2-PD.raw
SUBJECT_SAMPLE_FACTORS           	9013	9013-2-PE	Study_Diet:Modulen	Type=Plasma; Age=40; Sex=Male; BMI=24.1; Ethnicity=Hispanic or Latino; Race=White; Time=Baseline; RAW_FILE_NAME=0073_WUG_FARMM_HIL-9013-2-PE.raw 0073_WUG_FARMM_LIP-9013-2-PE.raw 0073_WU_FARMM_CMH_9013-2-PE.raw 0073_WUG_FARMM_FFA-9013-2-PE.raw
SUBJECT_SAMPLE_FACTORS           	9014	9014-3-PA	Study_Diet:Vegan	Type=Plasma; Age=23; Sex=Female; BMI=20.9; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 15; RAW_FILE_NAME=0010_WUG_FARMM_HIL-9014-3-PA.raw 0010_WUG_FARMM_LIP-9014-3-PA.raw 0010_WU_FARMM_CMH_9014-3-PA.raw 0010_WUG_FARMM_FFA-9014-3-PA.raw
SUBJECT_SAMPLE_FACTORS           	9014	9014-3-PB	Study_Diet:Vegan	Type=Plasma; Age=23; Sex=Female; BMI=20.9; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 12; RAW_FILE_NAME=0008_WUG_FARMM_HIL-9014-3-PB.raw 0008_WUG_FARMM_LIP-9014-3-PB.raw 0008_WU_FARMM_CMH_9014-3-PB.raw 0008_WUG_FARMM_FFA-9014-3-PB.raw
SUBJECT_SAMPLE_FACTORS           	9014	9014-3-PC	Study_Diet:Vegan	Type=Plasma; Age=23; Sex=Female; BMI=20.9; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 9; RAW_FILE_NAME=0006_WUG_FARMM_HIL-9014-3-PC.raw 0006_WUG_FARMM_LIP-9014-3-PC.raw 0006_WU_FARMM_CMH_9014-3-PC.raw 0006_WUG_FARMM_FFA-9014-3-PC.raw
SUBJECT_SAMPLE_FACTORS           	9014	9014-3-PD	Study_Diet:Vegan	Type=Plasma; Age=23; Sex=Female; BMI=20.9; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 5; RAW_FILE_NAME=0009_WUG_FARMM_HIL-9014-3-PD.raw 0009_WUG_FARMM_LIP-9014-3-PD.raw 0009_WU_FARMM_CMH_9014-3-PD.raw 0009_WUG_FARMM_FFA-9014-3-PD.raw
SUBJECT_SAMPLE_FACTORS           	9014	9014-3-PE	Study_Diet:Vegan	Type=Plasma; Age=23; Sex=Female; BMI=20.9; Ethnicity=Not Hispanic or Latino; Race=White; Time=Baseline; RAW_FILE_NAME=0007_WUG_FARMM_HIL-9014-3-PE.raw 0007_WUG_FARMM_LIP-9014-3-PE.raw 0007_WU_FARMM_CMH_9014-3-PE.raw 0007_WUG_FARMM_FFA-9014-3-PE.raw
SUBJECT_SAMPLE_FACTORS           	9015	9015-1-PA	Study_Diet:Western	Type=Plasma; Age=23; Sex=Female; BMI=20.5; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 15; RAW_FILE_NAME=0097_WUG_FARMM_HIL-9015-1-PA.raw 0097_WUG_FARMM_LIP-9015-1-PA.raw 0097_WU_FARMM_CMH_9015-1-PA.raw 0097_WUG_FARMM_FFA-9015-1-PA.raw
SUBJECT_SAMPLE_FACTORS           	9015	9015-1-PB	Study_Diet:Western	Type=Plasma; Age=23; Sex=Female; BMI=20.5; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 12; RAW_FILE_NAME=0096_WUG_FARMM_HIL-9015-1-PB.raw 0096_WUG_FARMM_LIP-9015-1-PB.raw 0096_WU_FARMM_CMH_9015-1-PB.raw 0096_WUG_FARMM_FFA-9015-1-PB.raw
SUBJECT_SAMPLE_FACTORS           	9015	9015-1-PC	Study_Diet:Western	Type=Plasma; Age=23; Sex=Female; BMI=20.5; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 9; RAW_FILE_NAME=0100_WUG_FARMM_HIL-9015-1-PC.raw 0100_WUG_FARMM_LIP-9015-1-PC.raw 0100_WU_FARMM_CMH_9015-1-PC.raw 0100_WUG_FARMM_FFA-9015-1-PC.raw
SUBJECT_SAMPLE_FACTORS           	9015	9015-1-PD	Study_Diet:Western	Type=Plasma; Age=23; Sex=Female; BMI=20.5; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 5; RAW_FILE_NAME=0099_WUG_FARMM_HIL-9015-1-PD.raw 0099_WUG_FARMM_LIP-9015-1-PD.raw 0099_WU_FARMM_CMH_9015-1-PD.raw 0099_WUG_FARMM_FFA-9015-1-PD.raw
SUBJECT_SAMPLE_FACTORS           	9015	9015-1-PE	Study_Diet:Western	Type=Plasma; Age=23; Sex=Female; BMI=20.5; Ethnicity=Not Hispanic or Latino; Race=White; Time=Baseline; RAW_FILE_NAME=0098_WUG_FARMM_HIL-9015-1-PE.raw 0098_WUG_FARMM_LIP-9015-1-PE.raw 0098_WU_FARMM_CMH_9015-1-PE.raw 0098_WUG_FARMM_FFA-9015-1-PE.raw
SUBJECT_SAMPLE_FACTORS           	9016	9016-2-PA	Study_Diet:Modulen	Type=Plasma; Age=38; Sex=Female; BMI=25.6; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 15; RAW_FILE_NAME=0067_WUG_FARMM_HIL-9016-2-PA.raw 0067_WUG_FARMM_LIP-9016-2-PA.raw 0067_WU_FARMM_CMH_9016-2-PA.raw 0067_WUG_FARMM_FFA-9016-2-PA.raw
SUBJECT_SAMPLE_FACTORS           	9016	9016-2-PB	Study_Diet:Modulen	Type=Plasma; Age=38; Sex=Female; BMI=25.6; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 12; RAW_FILE_NAME=0066_WUG_FARMM_HIL-9016-2-PB.raw 0066_WUG_FARMM_LIP-9016-2-PB.raw 0066_WU_FARMM_CMH_9016-2-PB.raw 0066_WUG_FARMM_FFA-9016-2-PB.raw
SUBJECT_SAMPLE_FACTORS           	9016	9016-2-PC	Study_Diet:Modulen	Type=Plasma; Age=38; Sex=Female; BMI=25.6; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 9; RAW_FILE_NAME=0070_WUG_FARMM_HIL-9016-2-PC.raw 0070_WUG_FARMM_LIP-9016-2-PC.raw 0070_WU_FARMM_CMH_9016-2-PC.raw 0070_WUG_FARMM_FFA-9016-2-PC.raw
SUBJECT_SAMPLE_FACTORS           	9016	9016-2-PD	Study_Diet:Modulen	Type=Plasma; Age=38; Sex=Female; BMI=25.6; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 5; RAW_FILE_NAME=0068_WUG_FARMM_HIL-9016-2-PD.raw 0068_WUG_FARMM_LIP-9016-2-PD.raw 0068_WU_FARMM_CMH_9016-2-PD.raw 0068_WUG_FARMM_FFA-9016-2-PD.raw
SUBJECT_SAMPLE_FACTORS           	9016	9016-2-PE	Study_Diet:Modulen	Type=Plasma; Age=38; Sex=Female; BMI=25.6; Ethnicity=Not Hispanic or Latino; Race=White; Time=Baseline; RAW_FILE_NAME=0069_WUG_FARMM_HIL-9016-2-PE.raw 0069_WUG_FARMM_LIP-9016-2-PE.raw 0069_WU_FARMM_CMH_9016-2-PE.raw 0069_WUG_FARMM_FFA-9016-2-PE.raw
SUBJECT_SAMPLE_FACTORS           	9017	9017-3-PA	Study_Diet:Vegan	Type=Plasma; Age=25; Sex=Male; BMI=20.6; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 15; RAW_FILE_NAME=0057_WUG_FARMM_HIL-9017-3-PA.raw 0057_WUG_FARMM_LIP-9017-3-PA.raw 0057_WU_FARMM_CMH_9017-3-PA.raw 0057_WUG_FARMM_FFA-9017-3-PA.raw
SUBJECT_SAMPLE_FACTORS           	9017	9017-3-PB	Study_Diet:Vegan	Type=Plasma; Age=25; Sex=Male; BMI=20.6; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 12; RAW_FILE_NAME=0056_WUG_FARMM_HIL-9017-3-PB.raw 0056_WUG_FARMM_LIP-9017-3-PB.raw 0056_WU_FARMM_CMH_9017-3-PB.raw 0056_WUG_FARMM_FFA-9017-3-PB.raw
SUBJECT_SAMPLE_FACTORS           	9017	9017-3-PC	Study_Diet:Vegan	Type=Plasma; Age=25; Sex=Male; BMI=20.6; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 9; RAW_FILE_NAME=0058_WUG_FARMM_HIL-9017-3-PC.raw 0058_WUG_FARMM_LIP-9017-3-PC.raw 0058_WU_FARMM_CMH_9017-3-PC.raw 0058_WUG_FARMM_FFA-9017-3-PC.raw
SUBJECT_SAMPLE_FACTORS           	9017	9017-3-PD	Study_Diet:Vegan	Type=Plasma; Age=25; Sex=Male; BMI=20.6; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 5; RAW_FILE_NAME=0060_WUG_FARMM_HIL-9017-3-PD.raw 0060_WUG_FARMM_LIP-9017-3-PD.raw 0060_WU_FARMM_CMH_9017-3-PD.raw 0060_WUG_FARMM_FFA-9017-3-PD.raw
SUBJECT_SAMPLE_FACTORS           	9017	9017-3-PE	Study_Diet:Vegan	Type=Plasma; Age=25; Sex=Male; BMI=20.6; Ethnicity=Not Hispanic or Latino; Race=White; Time=Baseline; RAW_FILE_NAME=0059_WUG_FARMM_HIL-9017-3-PE.raw 0059_WUG_FARMM_LIP-9017-3-PE.raw 0059_WU_FARMM_CMH_9017-3-PE.raw 0059_WUG_FARMM_FFA-9017-3-PE.raw
SUBJECT_SAMPLE_FACTORS           	9019	9019-1-PA	Study_Diet:Western	Type=Plasma; Age=36; Sex=Male; BMI=21.8; Ethnicity=Not Hispanic or Latino; Race=Black/African American; Time=Day 15; RAW_FILE_NAME=0061_WUG_FARMM_HIL-9019-1-PA.raw 0061_WUG_FARMM_LIP-9019-1-PA.raw 0061_WU_FARMM_CMH_9019-1-PA.raw 0061_WUG_FARMM_FFA-9019-1-PA.raw
SUBJECT_SAMPLE_FACTORS           	9019	9019-1-PB	Study_Diet:Western	Type=Plasma; Age=36; Sex=Male; BMI=21.8; Ethnicity=Not Hispanic or Latino; Race=Black/African American; Time=Day 12; RAW_FILE_NAME=0063_WUG_FARMM_HIL-9019-1-PB.raw 0063_WUG_FARMM_LIP-9019-1-PB.raw 0063_WU_FARMM_CMH_9019-1-PB.raw 0063_WUG_FARMM_FFA-9019-1-PB.raw
SUBJECT_SAMPLE_FACTORS           	9019	9019-1-PC	Study_Diet:Western	Type=Plasma; Age=36; Sex=Male; BMI=21.8; Ethnicity=Not Hispanic or Latino; Race=Black/African American; Time=Day 9; RAW_FILE_NAME=0064_WUG_FARMM_HIL-9019-1-PC.raw 0064_WUG_FARMM_LIP-9019-1-PC.raw 0064_WU_FARMM_CMH_9019-1-PC.raw 0064_WUG_FARMM_FFA-9019-1-PC.raw
SUBJECT_SAMPLE_FACTORS           	9019	9019-1-PD	Study_Diet:Western	Type=Plasma; Age=36; Sex=Male; BMI=21.8; Ethnicity=Not Hispanic or Latino; Race=Black/African American; Time=Day 5; RAW_FILE_NAME=0062_WUG_FARMM_HIL-9019-1-PD.raw 0062_WUG_FARMM_LIP-9019-1-PD.raw 0062_WU_FARMM_CMH_9019-1-PD.raw 0062_WUG_FARMM_FFA-9019-1-PD.raw
SUBJECT_SAMPLE_FACTORS           	9019	9019-1-PE	Study_Diet:Western	Type=Plasma; Age=36; Sex=Male; BMI=21.8; Ethnicity=Not Hispanic or Latino; Race=Black/African American; Time=Baseline; RAW_FILE_NAME=0065_WUG_FARMM_HIL-9019-1-PE.raw 0065_WUG_FARMM_LIP-9019-1-PE.raw 0065_WU_FARMM_CMH_9019-1-PE.raw 0065_WUG_FARMM_FFA-9019-1-PE.raw
SUBJECT_SAMPLE_FACTORS           	9022	9022-3-PA	Study_Diet:Vegan	Type=Plasma; Age=25; Sex=Male; BMI=21.8; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 15; RAW_FILE_NAME=0005_WUG_FARMM_HIL-9022-3-PA.raw 0005_WUG_FARMM_LIP-9022-3-PA.raw 0005_WU_FARMM_CMH_9022-3-PA.raw 0005_WUG_FARMM_FFA-9022-3-PA.raw
SUBJECT_SAMPLE_FACTORS           	9022	9022-3-PB	Study_Diet:Vegan	Type=Plasma; Age=25; Sex=Male; BMI=21.8; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 12; RAW_FILE_NAME=0003_WUG_FARMM_HIL-9022-3-PB.raw 0003_WUG_FARMM_LIP-9022-3-PB.raw 0003_WU_FARMM_CMH_9022-3-PB.raw 0003_WUG_FARMM_FFA-9022-3-PB.raw
SUBJECT_SAMPLE_FACTORS           	9022	9022-3-PC	Study_Diet:Vegan	Type=Plasma; Age=25; Sex=Male; BMI=21.8; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 9; RAW_FILE_NAME=0004_WUG_FARMM_HIL-9022-3-PC.raw 0004_WUG_FARMM_LIP-9022-3-PC.raw 0004_WU_FARMM_CMH_9022-3-PC.raw 0004_WUG_FARMM_FFA-9022-3-PC.raw
SUBJECT_SAMPLE_FACTORS           	9022	9022-3-PD	Study_Diet:Vegan	Type=Plasma; Age=25; Sex=Male; BMI=21.8; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 5; RAW_FILE_NAME=0002_WUG_FARMM_HIL-9022-3-PD.raw 0002_WUG_FARMM_LIP-9022-3-PD.raw 0002_WU_FARMM_CMH_9022-3-PD.raw 0002_WUG_FARMM_FFA-9022-3-PD.raw
SUBJECT_SAMPLE_FACTORS           	9022	9022-3-PE	Study_Diet:Vegan	Type=Plasma; Age=25; Sex=Male; BMI=21.8; Ethnicity=Not Hispanic or Latino; Race=White; Time=Baseline; RAW_FILE_NAME=0001_WUG_FARMM_HIL-9022-3.raw 0001_WUG_FARMM_LIP-9022-3-PE.raw 0001_WU_FARMM_CMH_9022-3-PE.raw 0001_WUG_FARMM_FFA-9022-3-PE.raw
SUBJECT_SAMPLE_FACTORS           	9024	9024-2-PA	Study_Diet:Modulen	Type=Plasma; Age=27; Sex=Female; BMI=34.6; Ethnicity=Not Hispanic or Latino; Race=Black/African American; Time=Day 15; RAW_FILE_NAME=0012_WUG_FARMM_HIL-9024-2-PA.raw 0012_WUG_FARMM_LIP-9024-2-PA.raw 0012_WU_FARMM_CMH_9024-2-PA.raw 0012_WUG_FARMM_FFA-9024-2-PA.raw
SUBJECT_SAMPLE_FACTORS           	9024	9024-2-PB	Study_Diet:Modulen	Type=Plasma; Age=27; Sex=Female; BMI=34.6; Ethnicity=Not Hispanic or Latino; Race=Black/African American; Time=Day 12; RAW_FILE_NAME=0013_WUG_FARMM_HIL-9024-2-PB.raw 0013_WUG_FARMM_LIP-9024-2-PB.raw 0013_WU_FARMM_CMH_9024-2-PB.raw 0013_WUG_FARMM_FFA-9024-2-PB.raw
SUBJECT_SAMPLE_FACTORS           	9024	9024-2-PC	Study_Diet:Modulen	Type=Plasma; Age=27; Sex=Female; BMI=34.6; Ethnicity=Not Hispanic or Latino; Race=Black/African American; Time=Day 9; RAW_FILE_NAME=0015_WUG_FARMM_HIL-9024-2-PC.raw 0015_WUG_FARMM_LIP-9024-2-PC.raw 0015_WU_FARMM_CMH_9024-2-PC.raw 0015_WUG_FARMM_FFA-9024-2-PC.raw
SUBJECT_SAMPLE_FACTORS           	9024	9024-2-PD	Study_Diet:Modulen	Type=Plasma; Age=27; Sex=Female; BMI=34.6; Ethnicity=Not Hispanic or Latino; Race=Black/African American; Time=Day 5; RAW_FILE_NAME=0014_WUG_FARMM_HIL-9024-2-PD.raw 0014_WUG_FARMM_LIP-9024-2-PD.raw 0014_WU_FARMM_CMH_9024-2-PD.raw 0014_WUG_FARMM_FFA-9024-2-PD.raw
SUBJECT_SAMPLE_FACTORS           	9024	9024-2-PE	Study_Diet:Modulen	Type=Plasma; Age=27; Sex=Female; BMI=34.6; Ethnicity=Not Hispanic or Latino; Race=Black/African American; Time=Baseline; RAW_FILE_NAME=0011_WUG_FARMM_HIL-9024-2-PE.raw 0011_WUG_FARMM_LIP-9024-2-PE.raw 0011_WU_FARMM_CMH_9024-2-PE.raw 0011_WUG_FARMM_FFA-9024-2-PE.raw
SUBJECT_SAMPLE_FACTORS           	9025	9025-2-PA	Study_Diet:Modulen	Type=Plasma; Age=28; Sex=Male; BMI=30.8; Ethnicity=Not Hispanic or Latino; Race=Black/African American; Time=Day 15; RAW_FILE_NAME=0041_WUG_FARMM_HIL-9025-2-PA.raw 0041_WUG_FARMM_LIP-9025-2-PA.raw 0041_WU_FARMM_CMH_9025-2-PA.raw 0041_WUG_FARMM_FFA-9025-2-PA.raw
SUBJECT_SAMPLE_FACTORS           	9025	9025-2-PB	Study_Diet:Modulen	Type=Plasma; Age=28; Sex=Male; BMI=30.8; Ethnicity=Not Hispanic or Latino; Race=Black/African American; Time=Day 12; RAW_FILE_NAME=0045_WUG_FARMM_HIL-9025-2-PB.raw 0045_WUG_FARMM_LIP-9025-2-PB.raw 0045_WU_FARMM_CMH_9025-2-PB.raw 0045_WUG_FARMM_FFA-9025-2-PB.raw
SUBJECT_SAMPLE_FACTORS           	9025	9025-2-PC	Study_Diet:Modulen	Type=Plasma; Age=28; Sex=Male; BMI=30.8; Ethnicity=Not Hispanic or Latino; Race=Black/African American; Time=Day 9; RAW_FILE_NAME=0043_WUG_FARMM_HIL-9025-2-PC.raw 0043_WUG_FARMM_LIP-9025-2-PC.raw 0043_WU_FARMM_CMH_9025-2-PC.raw 0043_WUG_FARMM_FFA-9025-2-PC.raw
SUBJECT_SAMPLE_FACTORS           	9025	9025-2-PD	Study_Diet:Modulen	Type=Plasma; Age=28; Sex=Male; BMI=30.8; Ethnicity=Not Hispanic or Latino; Race=Black/African American; Time=Day 5; RAW_FILE_NAME=0044_WUG_FARMM_HIL-9025-2-PD.raw 0044_WUG_FARMM_LIP-9025-2-PD.raw 0044_WU_FARMM_CMH_9025-2-PD.raw 0044_WUG_FARMM_FFA-9025-2-PD.raw
SUBJECT_SAMPLE_FACTORS           	9025	9025-2-PE	Study_Diet:Modulen	Type=Plasma; Age=28; Sex=Male; BMI=30.8; Ethnicity=Not Hispanic or Latino; Race=Black/African American; Time=Baseline; RAW_FILE_NAME=0042_WUG_FARMM_HIL-9025-2-PE.raw 0042_WUG_FARMM_LIP-9025-2-PE.raw 0042_WU_FARMM_CMH_9025-2-PE.raw 0042_WUG_FARMM_FFA-9025-2-PE.raw
SUBJECT_SAMPLE_FACTORS           	9026	9026-3-PA	Study_Diet:Vegan	Type=Plasma; Age=24; Sex=Female; BMI=21.3; Ethnicity=Not Hispanic or Latino; Race=Black/African American; Time=Day 15; RAW_FILE_NAME=0039_WUG_FARMM_HIL-9026-3-PA.raw 0039_WUG_FARMM_LIP-9026-3-PA.raw 0039_WU_FARMM_CMH_9026-3-PA.raw 0039_WUG_FARMM_FFA-9026-3-PA.raw
SUBJECT_SAMPLE_FACTORS           	9026	9026-3-PB	Study_Diet:Vegan	Type=Plasma; Age=24; Sex=Female; BMI=21.3; Ethnicity=Not Hispanic or Latino; Race=Black/African American; Time=Day 12; RAW_FILE_NAME=0040_WUG_FARMM_HIL-9026-3-PB.raw 0040_WUG_FARMM_LIP-9026-3-PB.raw 0040_WU_FARMM_CMH_9026-3-PB.raw 0040_WUG_FARMM_FFA-9026-3-PB.raw
SUBJECT_SAMPLE_FACTORS           	9026	9026-3-PC	Study_Diet:Vegan	Type=Plasma; Age=24; Sex=Female; BMI=21.3; Ethnicity=Not Hispanic or Latino; Race=Black/African American; Time=Day 9; RAW_FILE_NAME=0038_WUG_FARMM_HIL-9026-3-PC.raw 0038_WUG_FARMM_LIP-9026-3-PC.raw 0038_WU_FARMM_CMH_9026-3-PC.raw 0038_WUG_FARMM_FFA-9026-3-PC.raw
SUBJECT_SAMPLE_FACTORS           	9026	9026-3-PD	Study_Diet:Vegan	Type=Plasma; Age=24; Sex=Female; BMI=21.3; Ethnicity=Not Hispanic or Latino; Race=Black/African American; Time=Day 5; RAW_FILE_NAME=0036_WUG_FARMM_HIL-9026-3-PD.raw 0036_WUG_FARMM_LIP-9026-3-PD.raw 0036_WU_FARMM_CMH_9026-3-PD.raw 0036_WUG_FARMM_FFA-9026-3-PD.raw
SUBJECT_SAMPLE_FACTORS           	9026	9026-3-PE	Study_Diet:Vegan	Type=Plasma; Age=24; Sex=Female; BMI=21.3; Ethnicity=Not Hispanic or Latino; Race=Black/African American; Time=Baseline; RAW_FILE_NAME=0037_WUG_FARMM_HIL-9026-3-PE.raw 0037_WUG_FARMM_LIP-9026-3-PE.raw 0037_WU_FARMM_CMH_9026-3-PE.raw 0037_WUG_FARMM_FFA-9026-3-PE.raw
SUBJECT_SAMPLE_FACTORS           	9027	9027-3-PA	Study_Diet:Vegan	Type=Plasma; Age=22; Sex=Female; BMI=25.1; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 15; RAW_FILE_NAME=0030_WUG_FARMM_HIL-9027-3-PA.raw 0030_WUG_FARMM_LIP-9027-3-PA.raw 0030_WU_FARMM_CMH_9027-3-PA.raw 0030_WUG_FARMM_FFA-9027-3-PA.raw
SUBJECT_SAMPLE_FACTORS           	9027	9027-3-PB	Study_Diet:Vegan	Type=Plasma; Age=22; Sex=Female; BMI=25.1; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 12; RAW_FILE_NAME=0028_WUG_FARMM_HIL-9027-3-PB.raw 0028_WUG_FARMM_LIP-9027-3-PB.raw 0028_WU_FARMM_CMH_9027-3-PB.raw 0028_WUG_FARMM_FFA-9027-3-PB.raw
SUBJECT_SAMPLE_FACTORS           	9027	9027-3-PC	Study_Diet:Vegan	Type=Plasma; Age=22; Sex=Female; BMI=25.1; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 9; RAW_FILE_NAME=0029_WUG_FARMM_HIL-9027-3-PC.raw 0029_WUG_FARMM_LIP-9027-3-PC.raw 0029_WU_FARMM_CMH_9027-3-PC.raw 0029_WUG_FARMM_FFA-9027-3-PC.raw
SUBJECT_SAMPLE_FACTORS           	9027	9027-3-PD	Study_Diet:Vegan	Type=Plasma; Age=22; Sex=Female; BMI=25.1; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 5; RAW_FILE_NAME=0026_WUG_FARMM_HIL-9027-3-PD.raw 0026_WUG_FARMM_LIP-9027-3-PD.raw 0026_WU_FARMM_CMH_9027-3-PD.raw 0026_WUG_FARMM_FFA-9027-3-PD.raw
SUBJECT_SAMPLE_FACTORS           	9027	9027-3-PE	Study_Diet:Vegan	Type=Plasma; Age=22; Sex=Female; BMI=25.1; Ethnicity=Not Hispanic or Latino; Race=White; Time=Baseline; RAW_FILE_NAME=0027_WUG_FARMM_HIL-9027-3-PE.raw 0027_WUG_FARMM_LIP-9027-3-PE.raw 0027_WU_FARMM_CMH_9027-3-PE.raw 0027_WUG_FARMM_FFA-9027-3-PE.raw
SUBJECT_SAMPLE_FACTORS           	9029	9029-2-PA	Study_Diet:Modulen	Type=Plasma; Age=26; Sex=Male; BMI=35.4; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 15; RAW_FILE_NAME=0128_WUG_FARMM_HIL-9029-2-PA.raw 0128_WUG_FARMM_LIP-9029-2-PA.raw 0128_WU_FARMM_CMH_9029-2-PA.raw 0128_WUG_FARMM_FFA-9029-2-PA.raw
SUBJECT_SAMPLE_FACTORS           	9029	9029-2-PB	Study_Diet:Modulen	Type=Plasma; Age=26; Sex=Male; BMI=35.4; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 12; RAW_FILE_NAME=0126_WUG_FARMM_HIL-9029-2-PB.raw 0126_WUG_FARMM_LIP-9029-2-PB.raw 0126_WU_FARMM_CMH_9029-2-PB.raw 0126_WUG_FARMM_FFA-9029-2-PB.raw
SUBJECT_SAMPLE_FACTORS           	9029	9029-2-PC	Study_Diet:Modulen	Type=Plasma; Age=26; Sex=Male; BMI=35.4; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 9; RAW_FILE_NAME=0130_WUG_FARMM_HIL-9029-2-PC.raw 0130_WUG_FARMM_LIP-9029-2-PC.raw 0130_WU_FARMM_CMH_9029-2-PC.raw 0130_WUG_FARMM_FFA-9029-2-PC.raw
SUBJECT_SAMPLE_FACTORS           	9029	9029-2-PD	Study_Diet:Modulen	Type=Plasma; Age=26; Sex=Male; BMI=35.4; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 5; RAW_FILE_NAME=0127_WUG_FARMM_HIL-9029-2-PD.raw 0127_WUG_FARMM_LIP-9029-2-PD.raw 0127_WU_FARMM_CMH_9029-2-PD.raw 0127_WUG_FARMM_FFA-9029-2-PD.raw
SUBJECT_SAMPLE_FACTORS           	9029	9029-2-PE	Study_Diet:Modulen	Type=Plasma; Age=26; Sex=Male; BMI=35.4; Ethnicity=Not Hispanic or Latino; Race=White; Time=Baseline; RAW_FILE_NAME=0129_WUG_FARMM_HIL-9029-2-PE.raw 0129_WUG_FARMM_LIP-9029-2-PE.raw 0129_WU_FARMM_CMH_9029-2-PE.raw 0129_WUG_FARMM_FFA-9029-2-PE.raw
SUBJECT_SAMPLE_FACTORS           	9030	9030-1-PA	Study_Diet:Western	Type=Plasma; Age=46; Sex=Male; BMI=27.7; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 15; RAW_FILE_NAME=0053_WUG_FARMM_HIL-9030-1-PA.raw 0053_WUG_FARMM_LIP-9030-1-PA.raw 0053_WU_FARMM_CMH_9030-1-PA.raw 0053_WUG_FARMM_FFA-9030-1-PA.raw
SUBJECT_SAMPLE_FACTORS           	9030	9030-1-PB	Study_Diet:Western	Type=Plasma; Age=46; Sex=Male; BMI=27.7; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 12; RAW_FILE_NAME=0054_WUG_FARMM_HIL-9030-1-PB.raw 0054_WUG_FARMM_LIP-9030-1-PB.raw 0054_WU_FARMM_CMH_9030-1-PB.raw 0054_WUG_FARMM_FFA-9030-1-PB.raw
SUBJECT_SAMPLE_FACTORS           	9030	9030-1-PC	Study_Diet:Western	Type=Plasma; Age=46; Sex=Male; BMI=27.7; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 9; RAW_FILE_NAME=0055_WUG_FARMM_HIL-9030-1-PC.raw 0055_WUG_FARMM_LIP-9030-1-PC.raw 0055_WU_FARMM_CMH_9030-1-PC.raw 0055_WUG_FARMM_FFA-9030-1-PC.raw
SUBJECT_SAMPLE_FACTORS           	9030	9030-1-PD	Study_Diet:Western	Type=Plasma; Age=46; Sex=Male; BMI=27.7; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 5; RAW_FILE_NAME=0051_WUG_FARMM_HIL-9030-1-PD.raw 0051_WUG_FARMM_LIP-9030-1-PD.raw 0051_WU_FARMM_CMH_9030-1-PD.raw 0051_WUG_FARMM_FFA-9030-1-PD.raw
SUBJECT_SAMPLE_FACTORS           	9030	9030-1-PE	Study_Diet:Western	Type=Plasma; Age=46; Sex=Male; BMI=27.7; Ethnicity=Not Hispanic or Latino; Race=White; Time=Baseline; RAW_FILE_NAME=0052_WUG_FARMM_HIL-9030-1-PE.raw 0052_WUG_FARMM_LIP-9030-1-PE.raw 0052_WU_FARMM_CMH_9030-1-PE.raw 0052_WUG_FARMM_FFA-9030-1-PE.raw
SUBJECT_SAMPLE_FACTORS           	9031	9031-3-PA	Study_Diet:Vegan	Type=Plasma; Age=32; Sex=Female; BMI=22.8; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 15; RAW_FILE_NAME=0019_WUG_FARMM_HIL-9031-3-PA.raw 0019_WUG_FARMM_LIP-9031-3-PA.raw 0019_WU_FARMM_CMH_9031-3-PA.raw 0019_WUG_FARMM_FFA-9031-3-PA.raw
SUBJECT_SAMPLE_FACTORS           	9031	9031-3-PB	Study_Diet:Vegan	Type=Plasma; Age=32; Sex=Female; BMI=22.8; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 12; RAW_FILE_NAME=0018_WUG_FARMM_HIL-9031-3-PB.raw 0018_WUG_FARMM_LIP-9031-3-PB.raw 0018_WU_FARMM_CMH_9031-3-PB.raw 0018_WUG_FARMM_FFA-9031-3-PB.raw
SUBJECT_SAMPLE_FACTORS           	9031	9031-3-PC	Study_Diet:Vegan	Type=Plasma; Age=32; Sex=Female; BMI=22.8; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 9; RAW_FILE_NAME=0020_WUG_FARMM_HIL-9031-3-PC.raw 0020_WUG_FARMM_LIP-9031-3-PC.raw 0020_WU_FARMM_CMH_9031-3-PC.raw 0020_WUG_FARMM_FFA-9031-3-PC.raw
SUBJECT_SAMPLE_FACTORS           	9031	9031-3-PD	Study_Diet:Vegan	Type=Plasma; Age=32; Sex=Female; BMI=22.8; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 5; RAW_FILE_NAME=0016_WUG_FARMM_HIL-9031-3-PD.raw 0016_WUG_FARMM_LIP-9031-3-PD.raw 0016_WU_FARMM_CMH_9031-3-PD.raw 0016_WUG_FARMM_FFA-9031-3-PD.raw
SUBJECT_SAMPLE_FACTORS           	9031	9031-3-PE	Study_Diet:Vegan	Type=Plasma; Age=32; Sex=Female; BMI=22.8; Ethnicity=Not Hispanic or Latino; Race=White; Time=Baseline; RAW_FILE_NAME=0017_WUG_FARMM_HIL-9031-3-PE.raw 0017_WUG_FARMM_LIP-9031-3-PE.raw 0017_WU_FARMM_CMH_9031-3-PE.raw 0017_WUG_FARMM_FFA-9031-3-PE.raw
SUBJECT_SAMPLE_FACTORS           	9032	9032-2-PA	Study_Diet:Modulen	Type=Plasma; Age=53; Sex=Male; BMI=31; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 15; RAW_FILE_NAME=0111_WUG_FARMM_HIL-9032-2-PA.raw 0111_WUG_FARMM_LIP-9032-2-PA.raw 0111_WU_FARMM_CMH_9032-2-PA.raw 0111_WUG_FARMM_FFA-9032-2-PA.raw
SUBJECT_SAMPLE_FACTORS           	9032	9032-2-PB	Study_Diet:Modulen	Type=Plasma; Age=53; Sex=Male; BMI=31; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 12; RAW_FILE_NAME=0114_WUG_FARMM_HIL-9032-2-PB.raw 0114_WUG_FARMM_LIP-9032-2-PB.raw 0114_WU_FARMM_CMH_9032-2-PB.raw 0114_WUG_FARMM_FFA-9032-2-PB.raw
SUBJECT_SAMPLE_FACTORS           	9032	9032-2-PC	Study_Diet:Modulen	Type=Plasma; Age=53; Sex=Male; BMI=31; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 9; RAW_FILE_NAME=0112_WUG_FARMM_HIL-9032-2-PC.raw 0112_WUG_FARMM_LIP-9032-2-PC.raw 0112_WU_FARMM_CMH_9032-2-PC.raw 0112_WUG_FARMM_FFA-9032-2-PC.raw
SUBJECT_SAMPLE_FACTORS           	9032	9032-2-PD	Study_Diet:Modulen	Type=Plasma; Age=53; Sex=Male; BMI=31; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 5; RAW_FILE_NAME=0113_WUG_FARMM_HIL-9032-2-PD.raw 0113_WUG_FARMM_LIP-9032-2-PD.raw 0113_WU_FARMM_CMH_9032-2-PD.raw 0113_WUG_FARMM_FFA-9032-2-PD.raw
SUBJECT_SAMPLE_FACTORS           	9032	9032-2-PE	Study_Diet:Modulen	Type=Plasma; Age=53; Sex=Male; BMI=31; Ethnicity=Not Hispanic or Latino; Race=White; Time=Baseline; RAW_FILE_NAME=0115_WUG_FARMM_HIL-9032-2-PE.raw 0115_WUG_FARMM_LIP-9032-2-PE.raw 0115_WU_FARMM_CMH_9032-2-PE.raw 0115_WUG_FARMM_FFA-9032-2-PE.raw
SUBJECT_SAMPLE_FACTORS           	9033	9033-1-PA	Study_Diet:Western	Type=Plasma; Age=57; Sex=Male; BMI=27.1; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 15; RAW_FILE_NAME=0033_WUG_FARMM_HIL-9033-1-PA.raw 0033_WUG_FARMM_LIP-9033-1-PA.raw 0033_WU_FARMM_CMH_9033-1-PA.raw 0033_WUG_FARMM_FFA-9033-1-PA.raw
SUBJECT_SAMPLE_FACTORS           	9033	9033-1-PB	Study_Diet:Western	Type=Plasma; Age=57; Sex=Male; BMI=27.1; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 12; RAW_FILE_NAME=0031_WUG_FARMM_HIL-9033-1-PB.raw 0031_WUG_FARMM_LIP-9033-1-PB.raw 0031_WU_FARMM_CMH_9033-1-PB.raw 0031_WUG_FARMM_FFA-9033-1-PB.raw
SUBJECT_SAMPLE_FACTORS           	9033	9033-1-PC	Study_Diet:Western	Type=Plasma; Age=57; Sex=Male; BMI=27.1; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 9; RAW_FILE_NAME=0034_WUG_FARMM_HIL-9033-1-PC.raw 0034_WUG_FARMM_LIP-9033-1-PC.raw 0034_WU_FARMM_CMH_9033-1-PC.raw 0034_WUG_FARMM_FFA-9033-1-PC.raw
SUBJECT_SAMPLE_FACTORS           	9033	9033-1-PD	Study_Diet:Western	Type=Plasma; Age=57; Sex=Male; BMI=27.1; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 5; RAW_FILE_NAME=0035_WUG_FARMM_HIL-9033-1-PD.raw 0035_WUG_FARMM_LIP-9033-1-PD.raw 0035_WU_FARMM_CMH_9033-1-PD.raw 0035_WUG_FARMM_FFA-9033-1-PD.raw
SUBJECT_SAMPLE_FACTORS           	9033	9033-1-PE	Study_Diet:Western	Type=Plasma; Age=57; Sex=Male; BMI=27.1; Ethnicity=Not Hispanic or Latino; Race=White; Time=Baseline; RAW_FILE_NAME=0032_WUG_FARMM_HIL-9033-1-PE.raw 0032_WUG_FARMM_LIP-9033-1-PE.raw 0032_WU_FARMM_CMH_9033-1-PE.raw 0032_WUG_FARMM_FFA-9033-1-PE.raw
SUBJECT_SAMPLE_FACTORS           	9034	9034-2-PA	Study_Diet:Modulen	Type=Plasma; Age=43; Sex=Female; BMI=25.3; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 15; RAW_FILE_NAME=0024_WUG_FARMM_HIL-9034-2-PA.raw 0024_WUG_FARMM_LIP-9034-2-PA.raw 0024_WU_FARMM_CMH_9034-2-PA.raw 0024_WUG_FARMM_FFA-9034-2-PA.raw
SUBJECT_SAMPLE_FACTORS           	9034	9034-2-PB	Study_Diet:Modulen	Type=Plasma; Age=43; Sex=Female; BMI=25.3; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 12; RAW_FILE_NAME=0023_WUG_FARMM_HIL-9034-2-PB.raw 0023_WUG_FARMM_LIP-9034-2-PB.raw 0023_WU_FARMM_CMH_9034-2-PB.raw 0023_WUG_FARMM_FFA-9034-2-PB.raw
SUBJECT_SAMPLE_FACTORS           	9034	9034-2-PC	Study_Diet:Modulen	Type=Plasma; Age=43; Sex=Female; BMI=25.3; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 9; RAW_FILE_NAME=0025_WUG_FARMM_HIL-9034-2-PC.raw 0025_WUG_FARMM_LIP-9034-2-PC.raw 0025_WU_FARMM_CMH_9034-2-PC.raw 0025_WUG_FARMM_FFA-9034-2-PC.raw
SUBJECT_SAMPLE_FACTORS           	9034	9034-2-PD	Study_Diet:Modulen	Type=Plasma; Age=43; Sex=Female; BMI=25.3; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 5; RAW_FILE_NAME=0021_WUG_FARMM_HIL-9034-2-PD.raw 0021_WUG_FARMM_LIP-9034-2-PD.raw 0021_WU_FARMM_CMH_9034-2-PD.raw 0021_WUG_FARMM_FFA-9034-2-PD.raw
SUBJECT_SAMPLE_FACTORS           	9034	9034-2-PE	Study_Diet:Modulen	Type=Plasma; Age=43; Sex=Female; BMI=25.3; Ethnicity=Not Hispanic or Latino; Race=White; Time=Baseline; RAW_FILE_NAME=0022_WUG_FARMM_HIL-9034-2-PE.raw 0022_WUG_FARMM_LIP-9034-2-PE.raw 0022_WU_FARMM_CMH_9034-2-PE.raw 0022_WUG_FARMM_FFA-9034-2-PE.raw
SUBJECT_SAMPLE_FACTORS           	9035	9035-3-PA	Study_Diet:Vegan	Type=Plasma; Age=25; Sex=Male; BMI=19.7; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 15; RAW_FILE_NAME=0140_WUG_FARMM_HIL-9035-3-PA.raw 0140_WUG_FARMM_LIP-9035-3-PA.raw 0140_WU_FARMM_CMH_9035-3-PA.raw 0140_WUG_FARMM_FFA-9035-3-PA.raw
SUBJECT_SAMPLE_FACTORS           	9035	9035-3-PB	Study_Diet:Vegan	Type=Plasma; Age=25; Sex=Male; BMI=19.7; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 12; RAW_FILE_NAME=0138_WUG_FARMM_HIL-9035-3-PB.raw 0138_WUG_FARMM_LIP-9035-3-PB.raw 0138_WU_FARMM_CMH_9035-3-PB.raw 0138_WUG_FARMM_FFA-9035-3-PB.raw
SUBJECT_SAMPLE_FACTORS           	9035	9035-3-PC	Study_Diet:Vegan	Type=Plasma; Age=25; Sex=Male; BMI=19.7; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 9; RAW_FILE_NAME=0137_WUG_FARMM_HIL-9035-3-PC.raw 0137_WUG_FARMM_LIP-9035-3-PC.raw 0137_WU_FARMM_CMH_9035-3-PC.raw 0137_WUG_FARMM_FFA-9035-3-PC.raw
SUBJECT_SAMPLE_FACTORS           	9035	9035-3-PD	Study_Diet:Vegan	Type=Plasma; Age=25; Sex=Male; BMI=19.7; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 5; RAW_FILE_NAME=0136_WUG_FARMM_HIL-9035-3-PD.raw 0136_WUG_FARMM_LIP-9035-3-PD.raw 0136_WU_FARMM_CMH_9035-3-PD.raw 0136_WUG_FARMM_FFA-9035-3-PD.raw
SUBJECT_SAMPLE_FACTORS           	9035	9035-3-PE	Study_Diet:Vegan	Type=Plasma; Age=25; Sex=Male; BMI=19.7; Ethnicity=Not Hispanic or Latino; Race=White; Time=Baseline; RAW_FILE_NAME=0139_WUG_FARMM_HIL-9035-3-PE.raw 0139_WUG_FARMM_LIP-9035-3-PE.raw 0139_WU_FARMM_CMH_9035-3-PE.raw 0139_WUG_FARMM_FFA-9035-3-PE.raw
SUBJECT_SAMPLE_FACTORS           	9036	9036-1-PA	Study_Diet:Western	Type=Plasma; Age=60; Sex=Male; BMI=23.9; Ethnicity=Not Hispanic or Latino; Race=American Indian/Alaskan Native; Time=White; RAW_FILE_NAME=Day 15;0081_WUG_FARMM_HIL-9036-1-PA.raw 0081_WUG_FARMM_LIP-9036-1-PA.raw 0081_WU_FARMM_CMH_9036-1-PA.raw
SUBJECT_SAMPLE_FACTORS           	9036	9036-1-PB	Study_Diet:Western	Type=Plasma; Age=60; Sex=Male; BMI=23.9; Ethnicity=Not Hispanic or Latino; Race=American Indian/Alaskan Native; Time=White; RAW_FILE_NAME=Day 12;0083_WUG_FARMM_HIL-9036-1-PB.raw 0083_WUG_FARMM_LIP-9036-1-PB.raw 0083_WU_FARMM_CMH_9036-1-PB.raw
SUBJECT_SAMPLE_FACTORS           	9036	9036-1-PC	Study_Diet:Western	Type=Plasma; Age=60; Sex=Male; BMI=23.9; Ethnicity=Not Hispanic or Latino; Race=American Indian/Alaskan Native; Time=White; RAW_FILE_NAME=Day 9;0084_WUG_FARMM_HIL-9036-1-PC.raw 0084_WUG_FARMM_LIP-9036-1-PC.raw 0084_WU_FARMM_CMH_9036-1-PC.raw
SUBJECT_SAMPLE_FACTORS           	9036	9036-1-PD	Study_Diet:Western	Type=Plasma; Age=60; Sex=Male; BMI=23.9; Ethnicity=Not Hispanic or Latino; Race=American Indian/Alaskan Native; Time=White; RAW_FILE_NAME=Day 5;0085_WUG_FARMM_HIL-9036-1-PD.raw 0085_WUG_FARMM_LIP-9036-1-PD.raw 0085_WU_FARMM_CMH_9036-1-PD.raw
SUBJECT_SAMPLE_FACTORS           	9036	9036-1-PE	Study_Diet:Western	Type=Plasma; Age=60; Sex=Male; BMI=23.9; Ethnicity=Not Hispanic or Latino; Race=American Indian/Alaskan Native; Time=White; RAW_FILE_NAME=Baseline;0082_WUG_FARMM_HIL-9036-1-PE.raw 0082_WUG_FARMM_LIP-9036-1-PE.raw 0082_WU_FARMM_CMH_9036-1-PE.raw
SUBJECT_SAMPLE_FACTORS           	9037	9037-1-PA	Study_Diet:Western	Type=Plasma; Age=29; Sex=Male; BMI=23; Ethnicity=Hispanic or Latino; Race=Black/African American; Time=White; RAW_FILE_NAME=Day 15;0123_WUG_FARMM_HIL-9037-1-PA.raw 0123_WUG_FARMM_LIP-9037-1-PA.raw 0123_WU_FARMM_CMH_9037-1-PA.raw
SUBJECT_SAMPLE_FACTORS           	9037	9037-1-PB	Study_Diet:Western	Type=Plasma; Age=29; Sex=Male; BMI=23; Ethnicity=Hispanic or Latino; Race=Black/African American; Time=White; RAW_FILE_NAME=Day 12;0125_WUG_FARMM_HIL-9037-1-PB.raw 0125_WUG_FARMM_LIP-9037-1-PB.raw 0125_WU_FARMM_CMH_9037-1-PB.raw
SUBJECT_SAMPLE_FACTORS           	9037	9037-1-PC	Study_Diet:Western	Type=Plasma; Age=29; Sex=Male; BMI=23; Ethnicity=Hispanic or Latino; Race=Black/African American; Time=White; RAW_FILE_NAME=Day 9;0121_WUG_FARMM_HIL-9037-1-PC.raw 0121_WUG_FARMM_LIP-9037-1-PC.raw 0121_WU_FARMM_CMH_9037-1-PC.raw
SUBJECT_SAMPLE_FACTORS           	9037	9037-1-PD	Study_Diet:Western	Type=Plasma; Age=29; Sex=Male; BMI=23; Ethnicity=Hispanic or Latino; Race=Black/African American; Time=White; RAW_FILE_NAME=Day 5;0122_WUG_FARMM_HIL-9037-1-PD.raw 0122_WUG_FARMM_LIP-9037-1-PD.raw 0122_WU_FARMM_CMH_9037-1-PD.raw
SUBJECT_SAMPLE_FACTORS           	9037	9037-1-PE	Study_Diet:Western	Type=Plasma; Age=29; Sex=Male; BMI=23; Ethnicity=Hispanic or Latino; Race=Black/African American; Time=White; RAW_FILE_NAME=Baseline;0124_WUG_FARMM_HIL-9037-1-PE.raw 0124_WUG_FARMM_LIP-9037-1-PE.raw 0124_WU_FARMM_CMH_9037-1-PE.raw
SUBJECT_SAMPLE_FACTORS           	9038	9038-2-PA	Study_Diet:Modulen	Type=Plasma; Age=59; Sex=Female; BMI=34.3; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 15; RAW_FILE_NAME=0086_WUG_FARMM_HIL-9038-2-PA.raw 0086_WUG_FARMM_LIP-9038-2-PA.raw 0086_WU_FARMM_CMH_9038-2-PA.raw 0086_WUG_FARMM_FFA-9038-2-PA.raw
SUBJECT_SAMPLE_FACTORS           	9038	9038-2-PB	Study_Diet:Modulen	Type=Plasma; Age=59; Sex=Female; BMI=34.3; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 12; RAW_FILE_NAME=0090_WUG_FARMM_HIL-9038-2-PB.raw 0090_WUG_FARMM_LIP-9038-2-PB.raw 0090_WU_FARMM_CMH_9038-2-PB.raw 0090_WUG_FARMM_FFA-9038-2-PB.raw
SUBJECT_SAMPLE_FACTORS           	9038	9038-2-PC	Study_Diet:Modulen	Type=Plasma; Age=59; Sex=Female; BMI=34.3; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 9; RAW_FILE_NAME=0088_WUG_FARMM_HIL-9038-2-PC.raw 0088_WUG_FARMM_LIP-9038-2-PC.raw 0088_WU_FARMM_CMH_9038-2-PC.raw 0088_WUG_FARMM_FFA-9038-2-PC.raw
SUBJECT_SAMPLE_FACTORS           	9038	9038-2-PD	Study_Diet:Modulen	Type=Plasma; Age=59; Sex=Female; BMI=34.3; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 5; RAW_FILE_NAME=0089_WUG_FARMM_HIL-9038-2-PD.raw 0089_WUG_FARMM_LIP-9038-2-PD.raw 0089_WU_FARMM_CMH_9038-2-PD.raw 0089_WUG_FARMM_FFA-9038-2-PD.raw
SUBJECT_SAMPLE_FACTORS           	9038	9038-2-PE	Study_Diet:Modulen	Type=Plasma; Age=59; Sex=Female; BMI=34.3; Ethnicity=Not Hispanic or Latino; Race=White; Time=Baseline; RAW_FILE_NAME=0087_WUG_FARMM_HIL-9038-2-PE.raw 0087_WUG_FARMM_LIP-9038-2-PE.raw 0087_WU_FARMM_CMH_9038-2-PE.raw 0087_WUG_FARMM_FFA-9038-2-PE.raw
SUBJECT_SAMPLE_FACTORS           	9040	9040-1-PA	Study_Diet:Western	Type=Plasma; Age=22; Sex=Male; BMI=23.7; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 15; RAW_FILE_NAME=0120_WUG_FARMM_HIL-9040-1-PA.raw 0120_WUG_FARMM_LIP-9040-1-PA.raw 0120_WU_FARMM_CMH_9040-1-PA.raw 0120_WUG_FARMM_FFA-9040-1-PA.raw
SUBJECT_SAMPLE_FACTORS           	9040	9040-1-PB	Study_Diet:Western	Type=Plasma; Age=22; Sex=Male; BMI=23.7; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 12; RAW_FILE_NAME=0116_WUG_FARMM_HIL-9040-1-PB.raw 0116_WUG_FARMM_LIP-9040-1-PB.raw 0116_WU_FARMM_CMH_9040-1-PB.raw 0116_WUG_FARMM_FFA-9040-1-PB.raw
SUBJECT_SAMPLE_FACTORS           	9040	9040-1-PC	Study_Diet:Western	Type=Plasma; Age=22; Sex=Male; BMI=23.7; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 9; RAW_FILE_NAME=0119_WUG_FARMM_HIL-9040-1-PC.raw 0119_WUG_FARMM_LIP-9040-1-PC.raw 0119_WU_FARMM_CMH_9040-1-PC.raw 0119_WUG_FARMM_FFA-9040-1-PC.raw
SUBJECT_SAMPLE_FACTORS           	9040	9040-1-PD	Study_Diet:Western	Type=Plasma; Age=22; Sex=Male; BMI=23.7; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 5; RAW_FILE_NAME=0117_WUG_FARMM_HIL-9040-1-PD.raw 0117_WUG_FARMM_LIP-9040-1-PD.raw 0117_WU_FARMM_CMH_9040-1-PD.raw 0117_WUG_FARMM_FFA-9040-1-PD.raw
SUBJECT_SAMPLE_FACTORS           	9040	9040-1-PE	Study_Diet:Western	Type=Plasma; Age=22; Sex=Male; BMI=23.7; Ethnicity=Not Hispanic or Latino; Race=White; Time=Baseline; RAW_FILE_NAME=0118_WUG_FARMM_HIL-9040-1-PE.raw 0150f_0118ri_WUG_FARMM_LIP-9040-1-PE.raw 0118_WU_FARMM_CMH_9040-1-PE.raw 0118_WUG_FARMM_FFA-9040-1-PE.raw
SUBJECT_SAMPLE_FACTORS           	QC-pooled_plasma	QPP01	Study_Diet:NA	Type=Plasma; Age=NA; Sex=NA; BMI=NA; Ethnicity=NA; Race=NA; Time=NA; RAW_FILE_NAME=NA;NA;0000d_WU_FARMM_CMH_QPP-01.raw NA
SUBJECT_SAMPLE_FACTORS           	QC-pooled_plasma	QPP02	Study_Diet:NA	Type=Plasma; Age=NA; Sex=NA; BMI=NA; Ethnicity=NA; Race=NA; Time=NA; RAW_FILE_NAME=0020a_WUG_FARMM_HIL-QPP02.raw 0020a_WUG_FARMM_LIP-QCPP02.raw 0020a_WU_FARMM_CMH_QPP-02.raw 0020a_WUG_FARMM_FFA-QCPP02.raw
SUBJECT_SAMPLE_FACTORS           	QC-pooled_plasma	QPP03	Study_Diet:NA	Type=Plasma; Age=NA; Sex=NA; BMI=NA; Ethnicity=NA; Race=NA; Time=NA; RAW_FILE_NAME=0040a_WUG_FARMM_HIL-QPP03.raw NA;0040a_WU_FARMM_CMH_QPP-03.raw 0040a_WUG_FARMM_FFA-QCPP03.raw
SUBJECT_SAMPLE_FACTORS           	QC-pooled_plasma	QPP04	Study_Diet:NA	Type=Plasma; Age=NA; Sex=NA; BMI=NA; Ethnicity=NA; Race=NA; Time=NA; RAW_FILE_NAME=0060a_WUG_FARMM_HIL-QPP04.raw 0060a_WUG_FARMM_LIP-QCPP04.raw 0060a_WU_FARMM_CMH_QPP-04.raw 0060a_WUG_FARMM_FFA-QCPP04.raw
SUBJECT_SAMPLE_FACTORS           	QC-pooled_plasma	QPP05	Study_Diet:NA	Type=Plasma; Age=NA; Sex=NA; BMI=NA; Ethnicity=NA; Race=NA; Time=NA; RAW_FILE_NAME=0080a_WUG_FARMM_HIL-QPP05.raw 0080a_WUG_FARMM_LIP-QCPP05.raw 0080a_WU_FARMM_CMH_QPP-05.raw 0080a_WUG_FARMM_FFA-QCPP05.raw
SUBJECT_SAMPLE_FACTORS           	QC-pooled_plasma	QPP06	Study_Diet:NA	Type=Plasma; Age=NA; Sex=NA; BMI=NA; Ethnicity=NA; Race=NA; Time=NA; RAW_FILE_NAME=0100a_WUG_FARMM_HIL-QPP06.raw 0100a_WUG_FARMM_LIP-QCPP06.raw 0080d_WU_FARMM_CMH_QPP-06.raw 0100a_WUG_FARMM_FFA-QCPP06.raw
SUBJECT_SAMPLE_FACTORS           	QC-pooled_plasma	QPP07	Study_Diet:NA	Type=Plasma; Age=NA; Sex=NA; BMI=NA; Ethnicity=NA; Race=NA; Time=NA; RAW_FILE_NAME=0120a_WUG_FARMM_HIL-QPP07.raw NA;0100a_WU_FARMM_CMH_QPP-07.raw 0120a_WUG_FARMM_FFA-QCPP07.raw
SUBJECT_SAMPLE_FACTORS           	QC-pooled_plasma	QPP08	Study_Diet:NA	Type=Plasma; Age=NA; Sex=NA; BMI=NA; Ethnicity=NA; Race=NA; Time=NA; RAW_FILE_NAME=0140a_WUG_FARMM_HIL-QPP08.raw 0150a_WUG_FARMM_LIP-QCPP08.raw 0120a_WU_FARMM_CMH_QPP-08.raw 0150a_WUG_FARMM_FFA-QCPP08.raw
SUBJECT_SAMPLE_FACTORS           	QC-pooled_plasma	QPP09	Study_Diet:NA	Type=Plasma; Age=NA; Sex=NA; BMI=NA; Ethnicity=NA; Race=NA; Time=NA; RAW_FILE_NAME=0150a_WUG_FARMM_HIL-QPP09.raw NA;0140a_WU_FARMM_CMH_QPP-09.raw NA
SUBJECT_SAMPLE_FACTORS           	QC-pooled_plasma	QPP10	Study_Diet:NA	Type=Plasma; Age=NA; Sex=NA; BMI=NA; Ethnicity=NA; Race=NA; Time=NA; RAW_FILE_NAME=NA;NA;0150a_WU_FARMM_CMH_QPP-10.raw NA
#COLLECTION
CO:COLLECTION_SUMMARY            	The first stool sample of each day of the inpatient groups was collected,
CO:COLLECTION_SUMMARY            	aliquoted and frozen immediately at -80oC. Blood was collected on days 1, 5, 9,
CO:COLLECTION_SUMMARY            	12 and 15 from which plasma aliquots were immediately isolated and frozen at
CO:COLLECTION_SUMMARY            	-80oC. The outpatient participants following the vegan diet also had blood
CO:COLLECTION_SUMMARY            	collected on days 1, 5, 9, 12, and 15 from which plasma aliquots were
CO:COLLECTION_SUMMARY            	immediately isolated and frozen at -80oC. Among these participants, the first
CO:COLLECTION_SUMMARY            	stool of the day was collected at home daily and kept on ice packs until it was
CO:COLLECTION_SUMMARY            	brought to the research unit where it could be aliquoted and frozen. Samples
CO:COLLECTION_SUMMARY            	from vegans were received for aliquoting within 24 hours and on average within 4
CO:COLLECTION_SUMMARY            	hours (Wu et al., 2010). Day 0 stool was not collected from vegans since their
CO:COLLECTION_SUMMARY            	diet did not change. The aliquoted amounts for all samples ranged from 500 mg to
CO:COLLECTION_SUMMARY            	1 g. The aliquots were taken from different areas of the sample. Remaining
CO:COLLECTION_SUMMARY            	sample was then collected into one residual 50 ml conical tube with a tongue
CO:COLLECTION_SUMMARY            	depressor. Any remaining stool was discarded. The University of Pennsylvania
CO:COLLECTION_SUMMARY            	Institutional Review Board (IRB) approved the research protocol.
CO:SAMPLE_TYPE                   	Blood (plasma)
CO:STORAGE_CONDITIONS            	-80℃
#TREATMENT
TR:TREATMENT_SUMMARY             	The 10 vegan participants continued to follow their usual diet as outpatients.
TR:TREATMENT_SUMMARY             	All participants completed the Diet History Questionnaire II (DHQ II), a food
TR:TREATMENT_SUMMARY             	frequency questionnaire developed by the Risk Factor Monitoring and Methods
TR:TREATMENT_SUMMARY             	Branch of the National Cancer Institute. The vegan participants also completed
TR:TREATMENT_SUMMARY             	three 24 hour diet recalls with a dietitian in the week prior to starting
TR:TREATMENT_SUMMARY             	antibiotics. 20 omnivores were randomly assigned to receive an omnivore diet or
TR:TREATMENT_SUMMARY             	EEN (Modulen® IBD) while residing in an inpatient research unit. The
TR:TREATMENT_SUMMARY             	macronutrient composition of Modulen® is protein 36g, fat 47g, and carbohydrate
TR:TREATMENT_SUMMARY             	110g per 1000 Kcal. The two omnivore diets were engineered to have a similar
TR:TREATMENT_SUMMARY             	composition to EEN. All the subjects consumed the menu A on day 11 and menu B on
TR:TREATMENT_SUMMARY             	days 4 and 14. Diets for omnivores were constructed to provide the expected
TR:TREATMENT_SUMMARY             	total calories required per day for the participant to maintain their current
TR:TREATMENT_SUMMARY             	weight and were adjusted if there was weight gain or loss of more than 2.5
TR:TREATMENT_SUMMARY             	pounds. On days 6, 7, and 8, inpatients participants received vancomycin 500mg
TR:TREATMENT_SUMMARY             	orally every 6 hours and neomycin 1000mg orally every 6 hours. On day 7,
TR:TREATMENT_SUMMARY             	participants consumed 4L of polyethylene glycol (PEG) based bowel purgative
TR:TREATMENT_SUMMARY             	(GoLytely®). Participants in the omnivore and EEN arms left the inpatient
TR:TREATMENT_SUMMARY             	research unit only under the direct supervision of a research staff member. The
TR:TREATMENT_SUMMARY             	vegan outpatients reported to the hospital twice on days 6,7 and 8 to receive
TR:TREATMENT_SUMMARY             	antibiotics and to consume PEG on day 7.
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	Plasma samples were thawed on ice and aliquoted for metabolite profiling
SP:SAMPLEPREP_SUMMARY            	analyses. Plasma samples were thawed and aliquoted for each LC-MS method. LC-MS
SP:SAMPLEPREP_SUMMARY            	samples were prepared for four profiling methods as follows: HILIC-pos:
SP:SAMPLEPREP_SUMMARY            	Metabolites were extracted by adding 90 μL of 74.9:24.9:0.2 v/v/v
SP:SAMPLEPREP_SUMMARY            	acetonitrile/methanol/formic acid containing stable isotope-labeled internal
SP:SAMPLEPREP_SUMMARY            	standards (valine-d8, Isotec; and phenylalanine-d8, Cambridge Isotope
SP:SAMPLEPREP_SUMMARY            	Laboratories; Andover, MA) to a 10 μL aliquot of stool homogenate or plasma.
SP:SAMPLEPREP_SUMMARY            	Samples were vortexed and then centrifuged (10 min, 9,000 x g, 4°C) to pellet
SP:SAMPLEPREP_SUMMARY            	protein precipitates. Supernatants were transferred to glass autosampler vials
SP:SAMPLEPREP_SUMMARY            	containing inserts for LC-MS analysis. C8-pos: Lipids were extracted by adding
SP:SAMPLEPREP_SUMMARY            	190 μL of isopropanol containing
SP:SAMPLEPREP_SUMMARY            	1-dodecanoyl-2-tridecanoyl-sn-glycero-3-phosphocholine as an internal standard
SP:SAMPLEPREP_SUMMARY            	(Avanti Polar Lipids; Alabaster, AL) to a 10 μL aliquot of stool homogenate or
SP:SAMPLEPREP_SUMMARY            	plasma. Samples were vortexed and then centrifuged (10 min, 9,000 x g, ambient
SP:SAMPLEPREP_SUMMARY            	temperature) to pellet protein precipitates. Supernatants were transferred to
SP:SAMPLEPREP_SUMMARY            	glass autosampler vials containing inserts for LC-MS analysis. HILIC-neg:
SP:SAMPLEPREP_SUMMARY            	Metabolites were extracted by adding 120 μL of 80% methanol containing
SP:SAMPLEPREP_SUMMARY            	inosine-15N4, thymine-d4 and glycocholate-d4 internal standards (Cambridge
SP:SAMPLEPREP_SUMMARY            	Isotope Laboratories; Andover, MA) to a 30 μL aliquot of stool homogenate or
SP:SAMPLEPREP_SUMMARY            	plasma. Samples were vortexed and then centrifuged (10 min, 9,000 x g, 4°C) to
SP:SAMPLEPREP_SUMMARY            	pellet protein precipitates. Supernatants were transferred to glass autosampler
SP:SAMPLEPREP_SUMMARY            	vials containing inserts for LC-MS analysis. C18-neg: Metabolites were extracted
SP:SAMPLEPREP_SUMMARY            	by adding 90 μL of methanol containing PGE2-d4 as an internal standard (Cayman
SP:SAMPLEPREP_SUMMARY            	Chemical Co.; Ann Arbor, MI) to a 30 μL aliquot of stool homogenate or plasma.
SP:SAMPLEPREP_SUMMARY            	Samples were vortexed and then centrifuged (10 min, 9,000 x g, 4°C) to pellet
SP:SAMPLEPREP_SUMMARY            	protein precipitates. Supernatants were transferred to glass autosampler vials
SP:SAMPLEPREP_SUMMARY            	containing inserts for LC-MS analysis.
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_SUMMARY        	C18-neg: high resolution and accurate mass profiling of metabolites of
CH:CHROMATOGRAPHY_SUMMARY        	intermediate polarity using reversed phase C18 chromatography and negative ion
CH:CHROMATOGRAPHY_SUMMARY        	mode full scan MS
CH:CHROMATOGRAPHY_TYPE           	Reversed phase
CH:INSTRUMENT_NAME               	Shimadzu Nexera X2
CH:COLUMN_NAME                   	Waters Acquity T3 (150 x 2.1 mm, 1.7 um)
CH:FLOW_GRADIENT                 	linear
CH:FLOW_RATE                     	450 uL/min
CH:COLUMN_TEMPERATURE            	45 ℃
CH:SOLVENT_A                     	0.01% Formic acid in water
CH:SOLVENT_B                     	0.01% Acetic acid in Acetonitrile
CH:INJECTION_TEMPERATURE         	4
#ANALYSIS
AN:ANALYSIS_TYPE                 	MS
#MS
MS:INSTRUMENT_NAME               	Thermo Q Exactive Orbitrap
MS:INSTRUMENT_TYPE               	Orbitrap
MS:MS_TYPE                       	ESI
MS:ION_MODE                      	NEGATIVE
MS:MS_COMMENTS                   	MS analyses were carried out using electrospray ionization in the negative ion
MS:MS_COMMENTS                   	mode using full scan analysis over m/z 70-850 at 70,000 resolution and 3 Hz data
MS:MS_COMMENTS                   	acquisition rate. Additional MS settings were: ion spray voltage, -3.5 kV;
MS:MS_COMMENTS                   	capillary temperature, 320°C; probe heater temperature, 300°C; sheath gas, 45;
MS:MS_COMMENTS                   	auxiliary gas, 10; and S-lens RF level 60All raw data were processed using
MS:MS_COMMENTS                   	Progenesis QI software (NonLinear Dynamics) for feature alignment, nontargeted
MS:MS_COMMENTS                   	signal detection, and signal integration. Targeted processing of a subset of
MS:MS_COMMENTS                   	known metabolites was conducted using TraceFinder software (Thermo Fisher
MS:MS_COMMENTS                   	Scientific; Waltham, MA). Compound identities were confirmed using reference
MS:MS_COMMENTS                   	standards.
MS:MS_RESULTS_FILE               	ST001522_AN002540_Results.txt	UNITS:unitless peak areas	Has m/z:Yes	Has RT:Yes	RT units:Minutes
#END