#METABOLOMICS WORKBENCH sven_haange_20210621_042152_mwtab.txt DATATRACK_ID:2707 STUDY_ID:ST001837 ANALYSIS_ID:AN002979 PROJECT_ID:000000 VERSION 1 CREATED_ON June 22, 2021, 7:37 pm #PROJECT PR:PROJECT_TITLE Fecal Transplant from Roux-Y-Gastric-Bypass rat model into a diet induced PR:PROJECT_TITLE obesity rat model PR:PROJECT_SUMMARY Investigating alterations in the intestinal microbiome metabolome and host blood PR:PROJECT_SUMMARY metabolome in a diet induced obesity (DIO) rat model after fecal transplant from PR:PROJECT_SUMMARY rats, which underwent Roux-Y-Gastric-Bypass surgery (RYGB). Experimental groups PR:PROJECT_SUMMARY for rat experiments. 1)Rats in the ‘RYGB_Donor group’ underwent RYGB PR:PROJECT_SUMMARY surgery. Postoperatively, animals received a two-choice diet, consisting of a SC PR:PROJECT_SUMMARY and HFD. Once the animals recovered and achieved a stabilized weight reduction PR:PROJECT_SUMMARY at 4 weeks postoperatively, body weight and food intake were recorded daily. PR:PROJECT_SUMMARY 2)Rats allocated into the ‘RYGB_AB group’ were handled the same as animals PR:PROJECT_SUMMARY in the ‘RYGB_donor group’. From postoperative week 4 onwards, animals PR:PROJECT_SUMMARY received an antibiotic treatment (AB) consisting of ampicillin (1 g/l; PR:PROJECT_SUMMARY Ratiopharm), vancomycin (0.5 g/l; Ratiopharm), neomycin (1 g/l; Bela-pharm), and PR:PROJECT_SUMMARY metronidazole (1 g/l; CP-Pharma), provided freshly every day via drinking PR:PROJECT_SUMMARY water.[19, 20] All antibiotics were given for the time period indicated in each PR:PROJECT_SUMMARY figure.[21] 3)Rats in the ‘Lean group’ served as healthy, age-matched PR:PROJECT_SUMMARY controls, constantly kept under SC. 4)Rats in the ‘Lean_AB group’ received PR:PROJECT_SUMMARY the same antibiotic treatment as animals in the RYGB_AB group, serving as a PR:PROJECT_SUMMARY control for antibiotic-specific side effects. All antibiotics were given for the PR:PROJECT_SUMMARY time periods indicated in each figure. 5)Rats in the ‘DIO group’ received no PR:PROJECT_SUMMARY surgery, but were otherwise handled like RYGB litter mates. 6)Rats in the PR:PROJECT_SUMMARY ‘DIO_FMT_RYGB group’ received fecal RYGB_Donor microbiota transplantation PR:PROJECT_SUMMARY once per week, but were otherwise handled like DIO litter mates. For fecal PR:PROJECT_SUMMARY transplantation experiments, 100 mg of fresh stool from body weight-stabilized PR:PROJECT_SUMMARY RYGB donors was re-suspended in 1 ml of PBS, homogenized carefully and PR:PROJECT_SUMMARY administered via oral gavage with 200 μl of the suspension. 7)Rats in the PR:PROJECT_SUMMARY ‘FMT_Lean group’ were handled like DIO litter mates, but were orally gavaged PR:PROJECT_SUMMARY with 200 μl of filtrate received from lean donors, and served as a control for PR:PROJECT_SUMMARY fecal transplantation and specificity of effect size(s) as a function of the PR:PROJECT_SUMMARY donor microbiota. 8)Rats in the ‘DIO_PF_FMT group’ were handled like DIO PR:PROJECT_SUMMARY litter mates, but pair-fed to the SC/HFD food intake of ‘FMT_Lean litter PR:PROJECT_SUMMARY mates, serving as a control for effects secondary to reduced caloric intake. PR:INSTITUTE Helmholtz Centre for Environmental Research - UFZ PR:LAST_NAME Haange PR:FIRST_NAME Sven PR:ADDRESS Permoserstrasse 1, Leipzig, Saxony, 04318, Germany PR:EMAIL sven.haange@ufz.de PR:PHONE 0049 341 2351099 #STUDY ST:STUDY_TITLE Quantitative total hexose study on blood serum and ceacal content of rat models ST:STUDY_SUMMARY Lipids were analyzed in blood serum and cecal content in rat models ST:INSTITUTE Helmholtz Centre for Environmental Research - UFZ ST:DEPARTMENT Department of Molecular Systems biology ST:LABORATORY Functional Metabolomics ST:LAST_NAME Rolle-Kampczyk ST:FIRST_NAME Ulrike ST:ADDRESS Permoserstrasse 15, 04318 Leipzig, Germany ST:EMAIL ulrike.rolle-kampczyk@ufz.de ST:STUDY_TYPE blood serum and caecum content ST:PHONE 0049 341 235 1537 #SUBJECT SU:SUBJECT_TYPE Mammal SU:SUBJECT_SPECIES Rattus norvegicus SU:TAXONOMY_ID 10116 #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data SUBJECT_SAMPLE_FACTORS - 1024035756 Tissue:Caecum | Sample treatment:DIO_AB Rat_number=47; Rat_sample_name=DIO_AB_7; RAW_FILE_NAME=KIT3-0-5504_1024035881_74_0_1_1_00_1024035756.wiff SUBJECT_SAMPLE_FACTORS - 1024035761 Tissue:Caecum | Sample treatment:FMT_lean Rat_number=48; Rat_sample_name=FMT_lean_1; RAW_FILE_NAME=KIT3-0-5504_1024035881_86_0_1_1_00_1024035761.wiff SUBJECT_SAMPLE_FACTORS - 1024035775 Tissue:Caecum | Sample treatment:DIO_AB Rat_number=49; Rat_sample_name=DIO_AB_5; RAW_FILE_NAME=KIT3-0-5504_1024035881_03_0_1_1_00_1024035775.wiff SUBJECT_SAMPLE_FACTORS - 1024041306 Tissue:Caecum | Sample treatment:Lean Rat_number=54; Rat_sample_name=Lean_5; RAW_FILE_NAME=KIT3-0-5504_1024035881_15_0_1_1_00_1024041306.wiff SUBJECT_SAMPLE_FACTORS - 1024041311 Tissue:Caecum | Sample treatment:Lean Rat_number=55; Rat_sample_name=Lean_6; RAW_FILE_NAME=KIT3-0-5504_1024035881_27_0_1_1_00_1024041311.wiff SUBJECT_SAMPLE_FACTORS - 1024041325 Tissue:Caecum | Sample treatment:DIO_FMT_RYGB Rat_number=57; Rat_sample_name=DIO_FMT_RYGB_8; RAW_FILE_NAME=KIT3-0-5504_1024035881_39_0_1_1_00_1024041325.wiff SUBJECT_SAMPLE_FACTORS - 1024041330 Tissue:Caecum | Sample treatment:FMT_lean Rat_number=58; Rat_sample_name=FMT_lean_2; RAW_FILE_NAME=KIT3-0-5504_1024035881_51_0_1_1_00_1024041330.wiff SUBJECT_SAMPLE_FACTORS - 1024041344 Tissue:Caecum | Sample treatment:FMT_lean Rat_number=63; Rat_sample_name=FMT_lean_3; RAW_FILE_NAME=KIT3-0-5504_1024035881_63_0_1_1_00_1024041344.wiff SUBJECT_SAMPLE_FACTORS - 1024041359 Tissue:Caecum | Sample treatment:FMT_lean Rat_number=64; Rat_sample_name=FMT_lean_4; RAW_FILE_NAME=KIT3-0-5504_1024035881_75_0_1_1_00_1024041359.wiff SUBJECT_SAMPLE_FACTORS - 1024041363 Tissue:Caecum | Sample treatment:DIO Rat_number=67; Rat_sample_name=DIO_11; RAW_FILE_NAME=KIT3-0-5504_1024035881_87_0_1_1_00_1024041363.wiff SUBJECT_SAMPLE_FACTORS - 1024041378 Tissue:Caecum | Sample treatment:DIO Rat_number=70; Rat_sample_name=DIO_12; RAW_FILE_NAME=KIT3-0-5504_1024035881_04_0_1_1_00_1024041378.wiff SUBJECT_SAMPLE_FACTORS - 1024041382 Tissue:Caecum | Sample treatment:DIO_FMT_RYGB Rat_number=72; Rat_sample_name=DIO_FMT_RYGB_7; RAW_FILE_NAME=KIT3-0-5504_1024035881_16_0_1_1_00_1024041382.wiff SUBJECT_SAMPLE_FACTORS - 1024041397 Tissue:Caecum | Sample treatment:FMT_lean Rat_number=73; Rat_sample_name=FMT_lean_5; RAW_FILE_NAME=KIT3-0-5504_1024035881_28_0_1_1_00_1024041397.wiff SUBJECT_SAMPLE_FACTORS - 1024041408 Tissue:Caecum | Sample treatment:FMT_lean Rat_number=74; Rat_sample_name=FMT_lean_6; RAW_FILE_NAME=KIT3-0-5504_1024035881_40_0_1_1_00_1024041408.wiff SUBJECT_SAMPLE_FACTORS - 1024035824 Tissue:Plasma | Sample treatment:Lean Rat_number=99; Rat_sample_name=Lean_7; RAW_FILE_NAME=KIT3-0-5504_1024035881_82_0_1_1_00_1024035824.wiff SUBJECT_SAMPLE_FACTORS - 1024038995 Tissue:Caecum | Sample treatment:Lean Rat_number=99; Rat_sample_name=Lean_7; RAW_FILE_NAME=KIT3-0-5504_1024035881_43_0_1_1_00_1024038995.wiff SUBJECT_SAMPLE_FACTORS - 1024035839 Tissue:Plasma | Sample treatment:Lean Rat_number=100; Rat_sample_name=Lean_8; RAW_FILE_NAME=KIT3-0-5504_1024035881_94_0_1_1_00_1024035839.wiff SUBJECT_SAMPLE_FACTORS - 1024039007 Tissue:Caecum | Sample treatment:Lean Rat_number=100; Rat_sample_name=Lean_8; RAW_FILE_NAME=KIT3-0-5504_1024035881_55_0_1_1_00_1024039007.wiff SUBJECT_SAMPLE_FACTORS - 1024032933 Tissue:Plasma | Sample treatment:RYGB_Donor Rat_number=106; Rat_sample_name=RYGB_Donor_9; RAW_FILE_NAME=KIT3-0-5504_1024035881_91_0_1_1_00_1024032933.wiff SUBJECT_SAMPLE_FACTORS - 1024041412 Tissue:Caecum | Sample treatment:RYGB_Donor Rat_number=106; Rat_sample_name=RYGB_Donor_9; RAW_FILE_NAME=KIT3-0-5504_1024035881_52_0_1_1_00_1024041412.wiff SUBJECT_SAMPLE_FACTORS - 1024032948 Tissue:Plasma | Sample treatment:RYGB_Donor Rat_number=107; Rat_sample_name=RYGB_Donor_7; RAW_FILE_NAME=KIT3-0-5504_1024035881_08_0_1_1_00_1024032948.wiff SUBJECT_SAMPLE_FACTORS - 1024041427 Tissue:Caecum | Sample treatment:RYGB_Donor Rat_number=107; Rat_sample_name=RYGB_Donor_7; RAW_FILE_NAME=KIT3-0-5504_1024035881_64_0_1_1_00_1024041427.wiff SUBJECT_SAMPLE_FACTORS - 1024032952 Tissue:Plasma | Sample treatment:RYGB_Donor Rat_number=109; Rat_sample_name=RYGB_Donor_8; RAW_FILE_NAME=KIT3-0-5504_1024035881_20_0_1_1_00_1024032952.wiff SUBJECT_SAMPLE_FACTORS - 1024041431 Tissue:Caecum | Sample treatment:RYGB_Donor Rat_number=109; Rat_sample_name=RYGB_Donor_8; RAW_FILE_NAME=KIT3-0-5504_1024035881_76_0_1_1_00_1024041431.wiff SUBJECT_SAMPLE_FACTORS - 1024032986 Tissue:Plasma | Sample treatment:RYGB_Donor Rat_number=114; Rat_sample_name=RYGB_Donor_10; RAW_FILE_NAME=KIT3-0-5504_1024035881_56_0_1_1_00_1024032986.wiff SUBJECT_SAMPLE_FACTORS - 1024041451 Tissue:Caecum | Sample treatment:RYGB_Donor Rat_number=114; Rat_sample_name=RYGB_Donor_10; RAW_FILE_NAME=KIT3-0-5504_1024035881_05_0_1_1_00_1024041451.wiff SUBJECT_SAMPLE_FACTORS - 1024032971 Tissue:Plasma | Sample treatment:RYGB_AB Rat_number=111; Rat_sample_name=RYGB_AB_9; RAW_FILE_NAME=KIT3-0-5504_1024035881_44_0_1_1_00_1024032971.wiff SUBJECT_SAMPLE_FACTORS - 1024041446 Tissue:Caecum | Sample treatment:RYGB_AB Rat_number=111; Rat_sample_name=RYGB_AB_9; RAW_FILE_NAME=KIT3-0-5504_1024035881_88_0_1_1_00_1024041446.wiff SUBJECT_SAMPLE_FACTORS - 1024032991 Tissue:Plasma | Sample treatment:RYGB_AB Rat_number=117; Rat_sample_name=RYGB_AB_7; RAW_FILE_NAME=KIT3-0-5504_1024035881_68_0_1_1_00_1024032991.wiff SUBJECT_SAMPLE_FACTORS - 1024041465 Tissue:Caecum | Sample treatment:RYGB_AB Rat_number=117; Rat_sample_name=RYGB_AB_7; RAW_FILE_NAME=KIT3-0-5504_1024035881_17_0_1_1_00_1024041465.wiff SUBJECT_SAMPLE_FACTORS - 1024033017 Tissue:Plasma | Sample treatment:RYGB_AB Rat_number=119; Rat_sample_name=RYGB_AB_8; RAW_FILE_NAME=KIT3-0-5504_1024035881_92_0_1_1_00_1024033017.wiff SUBJECT_SAMPLE_FACTORS - 1024041484 Tissue:Caecum | Sample treatment:RYGB_AB Rat_number=119; Rat_sample_name=RYGB_AB_8; RAW_FILE_NAME=KIT3-0-5504_1024035881_41_0_1_1_00_1024041484.wiff SUBJECT_SAMPLE_FACTORS - 1024033021 Tissue:Plasma | Sample treatment:DIO_FMT_RYGB Rat_number=122; Rat_sample_name=DIO_FMT_RYGB_10; RAW_FILE_NAME=KIT3-0-5504_1024035881_09_0_1_1_00_1024033021.wiff SUBJECT_SAMPLE_FACTORS - 1024038841 Tissue:Caecum | Sample treatment:DIO_FMT_RYGB Rat_number=122; Rat_sample_name=DIO_FMT_RYGB_10; RAW_FILE_NAME=KIT3-0-5504_1024035881_53_0_1_1_00_1024038841.wiff SUBJECT_SAMPLE_FACTORS - 1024033036 Tissue:Plasma | Sample treatment:DIO_FMT_RYGB Rat_number=123; Rat_sample_name=DIO_FMT_RYGB_9; RAW_FILE_NAME=KIT3-0-5504_1024035881_21_0_1_1_00_1024033036.wiff SUBJECT_SAMPLE_FACTORS - 1024038860 Tissue:Caecum | Sample treatment:DIO_FMT_RYGB Rat_number=123; Rat_sample_name=DIO_FMT_RYGB_9; RAW_FILE_NAME=KIT3-0-5504_1024035881_77_0_1_1_00_1024038860.wiff SUBJECT_SAMPLE_FACTORS - 1024033104 Tissue:Plasma | Sample treatment:DIO_PF_FMT Rat_number=124; Rat_sample_name=DIO_PF_FMT_5; RAW_FILE_NAME=KIT3-0-5504_1024035881_10_0_1_1_00_1024033104.wiff SUBJECT_SAMPLE_FACTORS - 1024038938 Tissue:Caecum | Sample treatment:DIO_PF_FMT Rat_number=124; Rat_sample_name=DIO_PF_FMT_5; RAW_FILE_NAME=KIT3-0-5504_1024035881_66_0_1_1_00_1024038938.wiff SUBJECT_SAMPLE_FACTORS - 1024038942 Tissue:Caecum | Sample treatment:DIO_PF_FMT Rat_number=125; Rat_sample_name=DIO_PF_FMT_6; RAW_FILE_NAME=KIT3-0-5504_1024035881_78_0_1_1_00_1024038942.wiff SUBJECT_SAMPLE_FACTORS - 1024033119 Tissue:Plasma | Sample treatment:DIO_PF_FMT Rat_number=125; Rat_sample_name=DIO_PF_FMT_6; RAW_FILE_NAME=KIT3-0-5504_1024035881_22_0_1_1_00_1024033119.wiff SUBJECT_SAMPLE_FACTORS - 1024033060 Tissue:Plasma | Sample treatment:DIO Rat_number=126; Rat_sample_name=DIO_8; RAW_FILE_NAME=KIT3-0-5504_1024035881_57_0_1_1_00_1024033060.wiff SUBJECT_SAMPLE_FACTORS - 1024038893 Tissue:Caecum | Sample treatment:DIO Rat_number=126; Rat_sample_name=DIO_8; RAW_FILE_NAME=KIT3-0-5504_1024035881_18_0_1_1_00_1024038893.wiff SUBJECT_SAMPLE_FACTORS - 1024033074 Tissue:Plasma | Sample treatment:DIO Rat_number=127; Rat_sample_name=DIO_9; RAW_FILE_NAME=KIT3-0-5504_1024035881_69_0_1_1_00_1024033074.wiff SUBJECT_SAMPLE_FACTORS - 1024038904 Tissue:Caecum | Sample treatment:DIO Rat_number=127; Rat_sample_name=DIO_9; RAW_FILE_NAME=KIT3-0-5504_1024035881_30_0_1_1_00_1024038904.wiff SUBJECT_SAMPLE_FACTORS - 1024033089 Tissue:Plasma | Sample treatment:DIO Rat_number=128; Rat_sample_name=DIO_7; RAW_FILE_NAME=KIT3-0-5504_1024035881_81_0_1_1_00_1024033089.wiff SUBJECT_SAMPLE_FACTORS - 1024038919 Tissue:Caecum | Sample treatment:DIO Rat_number=128; Rat_sample_name=DIO_7; RAW_FILE_NAME=KIT3-0-5504_1024035881_42_0_1_1_00_1024038919.wiff SUBJECT_SAMPLE_FACTORS - 1024033093 Tissue:Plasma | Sample treatment:DIO Rat_number=129; Rat_sample_name=DIO_10; RAW_FILE_NAME=KIT3-0-5504_1024035881_93_0_1_1_00_1024033093.wiff SUBJECT_SAMPLE_FACTORS - 1024038923 Tissue:Caecum | Sample treatment:DIO Rat_number=129; Rat_sample_name=DIO_10; RAW_FILE_NAME=KIT3-0-5504_1024035881_54_0_1_1_00_1024038923.wiff SUBJECT_SAMPLE_FACTORS - 1024033041 Tissue:Plasma | Sample treatment:DIO_FMT_RYGB Rat_number=130; Rat_sample_name=DIO_FMT_RYGB_11; RAW_FILE_NAME=KIT3-0-5504_1024035881_33_0_1_1_00_1024033041.wiff SUBJECT_SAMPLE_FACTORS - 1024038874 Tissue:Caecum | Sample treatment:DIO_FMT_RYGB Rat_number=130; Rat_sample_name=DIO_FMT_RYGB_11; RAW_FILE_NAME=KIT3-0-5504_1024035881_89_0_1_1_00_1024038874.wiff SUBJECT_SAMPLE_FACTORS - 1024033055 Tissue:Plasma | Sample treatment:DIO_FMT_RYGB Rat_number=131; Rat_sample_name=DIO_FMT_RYGB_12; RAW_FILE_NAME=KIT3-0-5504_1024035881_45_0_1_1_00_1024033055.wiff SUBJECT_SAMPLE_FACTORS - 1024038889 Tissue:Caecum | Sample treatment:DIO_FMT_RYGB Rat_number=131; Rat_sample_name=DIO_FMT_RYGB_12; RAW_FILE_NAME=KIT3-0-5504_1024035881_06_0_1_1_00_1024038889.wiff SUBJECT_SAMPLE_FACTORS - 1024035780 Tissue:Plasma | Sample treatment:DIO_PF_FMT Rat_number=132; Rat_sample_name=DIO_PF_FMT_1; RAW_FILE_NAME=KIT3-0-5504_1024035881_34_0_1_1_00_1024035780.wiff SUBJECT_SAMPLE_FACTORS - 1024038957 Tissue:Caecum | Sample treatment:DIO_PF_FMT Rat_number=132; Rat_sample_name=DIO_PF_FMT_1; RAW_FILE_NAME=KIT3-0-5504_1024035881_90_0_1_1_00_1024038957.wiff SUBJECT_SAMPLE_FACTORS - 1024035794 Tissue:Plasma | Sample treatment:DIO_PF_FMT Rat_number=133; Rat_sample_name=DIO_PF_FMT_4; RAW_FILE_NAME=KIT3-0-5504_1024035881_46_0_1_1_00_1024035794.wiff SUBJECT_SAMPLE_FACTORS - 1024038961 Tissue:Caecum | Sample treatment:DIO_PF_FMT Rat_number=133; Rat_sample_name=DIO_PF_FMT_4; RAW_FILE_NAME=KIT3-0-5504_1024035881_07_0_1_1_00_1024038961.wiff SUBJECT_SAMPLE_FACTORS - 1024035805 Tissue:Plasma | Sample treatment:DIO_PF_FMT Rat_number=134; Rat_sample_name=DIO_PF_FMT_2; RAW_FILE_NAME=KIT3-0-5504_1024035881_58_0_1_1_00_1024035805.wiff SUBJECT_SAMPLE_FACTORS - 1024038976 Tissue:Caecum | Sample treatment:DIO_PF_FMT Rat_number=134; Rat_sample_name=DIO_PF_FMT_2; RAW_FILE_NAME=KIT3-0-5504_1024035881_19_0_1_1_00_1024038976.wiff SUBJECT_SAMPLE_FACTORS - 1024035810 Tissue:Plasma | Sample treatment:DIO_PF_FMT Rat_number=135; Rat_sample_name=DIO_PF_FMT_3; RAW_FILE_NAME=KIT3-0-5504_1024035881_70_0_1_1_00_1024035810.wiff SUBJECT_SAMPLE_FACTORS - 1024038981 Tissue:Caecum | Sample treatment:DIO_PF_FMT Rat_number=135; Rat_sample_name=DIO_PF_FMT_3; RAW_FILE_NAME=KIT3-0-5504_1024035881_31_0_1_1_00_1024038981.wiff SUBJECT_SAMPLE_FACTORS - 1024035843 Tissue:Plasma | Sample treatment:Lean Rat_number=138; Rat_sample_name=Lean_9; RAW_FILE_NAME=KIT3-0-5504_1024035881_11_0_1_1_00_1024035843.wiff SUBJECT_SAMPLE_FACTORS - 1024039011 Tissue:Caecum | Sample treatment:Lean Rat_number=138; Rat_sample_name=Lean_9; RAW_FILE_NAME=KIT3-0-5504_1024035881_67_0_1_1_00_1024039011.wiff SUBJECT_SAMPLE_FACTORS - 1024039026 Tissue:Caecum | Sample treatment:Lean Rat_number=139; Rat_sample_name=Lean_10; RAW_FILE_NAME=KIT3-0-5504_1024035881_79_0_1_1_00_1024039026.wiff SUBJECT_SAMPLE_FACTORS - 1024035858 Tissue:Plasma | Sample treatment:Lean Rat_number=139; Rat_sample_name=Lean_10; RAW_FILE_NAME=KIT3-0-5504_1024035881_23_0_1_1_00_1024035858.wiff SUBJECT_SAMPLE_FACTORS - 1024032555 Tissue:Serum | Sample treatment:DIO Rat_number=1; Rat_sample_name=DIO_1; RAW_FILE_NAME=KIT3-0-5504_1024032812_74_0_1_1_00_1024032555.wiff SUBJECT_SAMPLE_FACTORS - 1024041219 Tissue:Caecum | Sample treatment:DIO Rat_number=1; Rat_sample_name=DIO_1; RAW_FILE_NAME=KIT3-0-5504_1024032812_80_0_1_1_00_1024041219.wiff SUBJECT_SAMPLE_FACTORS - 1024032560 Tissue:Serum | Sample treatment:DIO_AB Rat_number=2; Rat_sample_name=DIO_AB_1; RAW_FILE_NAME=KIT3-0-5504_1024032812_86_0_1_1_00_1024032560.wiff SUBJECT_SAMPLE_FACTORS - 1024041223 Tissue:Caecum | Sample treatment:DIO_AB Rat_number=2; Rat_sample_name=DIO_AB_1; RAW_FILE_NAME=KIT3-0-5504_1024032812_92_0_1_1_00_1024041223.wiff SUBJECT_SAMPLE_FACTORS - 1024032574 Tissue:Serum | Sample treatment:DIO_AB Rat_number=3; Rat_sample_name=DIO_AB_2; RAW_FILE_NAME=KIT3-0-5504_1024032812_03_0_1_1_00_1024032574.wiff SUBJECT_SAMPLE_FACTORS - 1024041238 Tissue:Caecum | Sample treatment:DIO_AB Rat_number=3; Rat_sample_name=DIO_AB_2; RAW_FILE_NAME=KIT3-0-5504_1024032812_09_0_1_1_00_1024041238.wiff SUBJECT_SAMPLE_FACTORS - 1024032589 Tissue:Serum | Sample treatment:DIO_AB Rat_number=4; Rat_sample_name=DIO_AB_3; RAW_FILE_NAME=KIT3-0-5504_1024032812_15_0_1_1_00_1024032589.wiff SUBJECT_SAMPLE_FACTORS - 1024041242 Tissue:Caecum | Sample treatment:DIO_AB Rat_number=4; Rat_sample_name=DIO_AB_3; RAW_FILE_NAME=KIT3-0-5504_1024032812_21_0_1_1_00_1024041242.wiff SUBJECT_SAMPLE_FACTORS - 1024032593 Tissue:Serum | Sample treatment:DIO_FMT_RYGB Rat_number=5; Rat_sample_name=DIO_FMT_RYGB_1; RAW_FILE_NAME=KIT3-0-5504_1024032812_27_0_1_1_00_1024032593.wiff SUBJECT_SAMPLE_FACTORS - 1024041257 Tissue:Caecum | Sample treatment:DIO_FMT_RYGB Rat_number=5; Rat_sample_name=DIO_FMT_RYGB_1; RAW_FILE_NAME=KIT3-0-5504_1024032812_33_0_1_1_00_1024041257.wiff SUBJECT_SAMPLE_FACTORS - 1024032604 Tissue:Serum | Sample treatment:DIO_AB Rat_number=6; Rat_sample_name=DIO_AB_4; RAW_FILE_NAME=KIT3-0-5504_1024032812_39_0_1_1_00_1024032604.wiff SUBJECT_SAMPLE_FACTORS - 1024041261 Tissue:Caecum | Sample treatment:DIO_AB Rat_number=6; Rat_sample_name=DIO_AB_4; RAW_FILE_NAME=KIT3-0-5504_1024032812_45_0_1_1_00_1024041261.wiff SUBJECT_SAMPLE_FACTORS - 1024041276 Tissue:Caecum | Sample treatment:DIO Rat_number=7; Rat_sample_name=DIO_2; RAW_FILE_NAME=KIT3-0-5504_1024032812_57_0_1_1_00_1024041276.wiff SUBJECT_SAMPLE_FACTORS - 1024032619 Tissue:Serum | Sample treatment:DIO_FMT_RYGB Rat_number=8; Rat_sample_name=DIO_FMT_RYGB_2; RAW_FILE_NAME=KIT3-0-5504_1024032812_51_0_1_1_00_1024032619.wiff SUBJECT_SAMPLE_FACTORS - 1024041281 Tissue:Caecum | Sample treatment:DIO_FMT_RYGB Rat_number=8; Rat_sample_name=DIO_FMT_RYGB_2; RAW_FILE_NAME=KIT3-0-5504_1024032812_69_0_1_1_00_1024041281.wiff SUBJECT_SAMPLE_FACTORS - 1024032623 Tissue:Serum | Sample treatment:DIO Rat_number=9; Rat_sample_name=DIO_3; RAW_FILE_NAME=KIT3-0-5504_1024032812_63_0_1_1_00_1024032623.wiff SUBJECT_SAMPLE_FACTORS - 1024041295 Tissue:Caecum | Sample treatment:DIO Rat_number=9; Rat_sample_name=DIO_3; RAW_FILE_NAME=KIT3-0-5504_1024032812_81_0_1_1_00_1024041295.wiff SUBJECT_SAMPLE_FACTORS - 1024032638 Tissue:Serum | Sample treatment:DIO Rat_number=10; Rat_sample_name=DIO_4; RAW_FILE_NAME=KIT3-0-5504_1024032812_75_0_1_1_00_1024032638.wiff SUBJECT_SAMPLE_FACTORS - 1024038651 Tissue:Caecum | Sample treatment:DIO Rat_number=10; Rat_sample_name=DIO_4; RAW_FILE_NAME=KIT3-0-5504_1024032812_93_0_1_1_00_1024038651.wiff SUBJECT_SAMPLE_FACTORS - 1024038666 Tissue:Caecum | Sample treatment:DIO_FMT_RYGB Rat_number=11; Rat_sample_name=DIO_FMT_RYGB_3; RAW_FILE_NAME=KIT3-0-5504_1024032812_10_0_1_1_00_1024038666.wiff SUBJECT_SAMPLE_FACTORS - 1024032642 Tissue:Serum | Sample treatment:DIO_FMT_RYGB Rat_number=11; Rat_sample_name=DIO_FMT_RYGB_3; RAW_FILE_NAME=KIT3-0-5504_1024032812_87_0_1_1_00_1024032642.wiff SUBJECT_SAMPLE_FACTORS - 1024032657 Tissue:Serum | Sample treatment:DIO_FMT_RYGB Rat_number=12; Rat_sample_name=DIO_FMT_RYGB_4; RAW_FILE_NAME=KIT3-0-5504_1024032812_04_0_1_1_00_1024032657.wiff SUBJECT_SAMPLE_FACTORS - 1024038671 Tissue:Caecum | Sample treatment:DIO_FMT_RYGB Rat_number=12; Rat_sample_name=DIO_FMT_RYGB_4; RAW_FILE_NAME=KIT3-0-5504_1024032812_22_0_1_1_00_1024038671.wiff SUBJECT_SAMPLE_FACTORS - 1024032661 Tissue:Serum | Sample treatment:RYGB_AB Rat_number=13; Rat_sample_name=RYGB_AB_2; RAW_FILE_NAME=KIT3-0-5504_1024032812_16_0_1_1_00_1024032661.wiff SUBJECT_SAMPLE_FACTORS - 1024038685 Tissue:Caecum | Sample treatment:RYGB_AB Rat_number=13; Rat_sample_name=RYGB_AB_2; RAW_FILE_NAME=KIT3-0-5504_1024032812_34_0_1_1_00_1024038685.wiff SUBJECT_SAMPLE_FACTORS - 1024032681 Tissue:Serum | Sample treatment:RYGB_Donor Rat_number=17; Rat_sample_name=RYGB_Donor_2; RAW_FILE_NAME=KIT3-0-5504_1024032812_40_0_1_1_00_1024032681.wiff SUBJECT_SAMPLE_FACTORS - 1024038701 Tissue:Caecum | Sample treatment:RYGB_Donor Rat_number=17; Rat_sample_name=RYGB_Donor_2; RAW_FILE_NAME=KIT3-0-5504_1024032812_58_0_1_1_00_1024038701.wiff SUBJECT_SAMPLE_FACTORS - 1024038715 Tissue:Caecum | Sample treatment:RYGB_Donor Rat_number=20; Rat_sample_name=RYGB_Donor_3; RAW_FILE_NAME=KIT3-0-5504_1024032812_70_0_1_1_00_1024038715.wiff SUBJECT_SAMPLE_FACTORS - 1024032706 Tissue:Serum | Sample treatment:RYGB_Donor Rat_number=20; Rat_sample_name=RYGB_Donor_3; RAW_FILE_NAME=KIT3-0-5504_1024032812_64_0_1_1_00_1024032706.wiff SUBJECT_SAMPLE_FACTORS - 1024032711 Tissue:Serum | Sample treatment:RYGB_AB Rat_number=23; Rat_sample_name=RYGB_AB_3; RAW_FILE_NAME=KIT3-0-5504_1024032812_76_0_1_1_00_1024032711.wiff SUBJECT_SAMPLE_FACTORS - 1024038720 Tissue:Caecum | Sample treatment:RYGB_AB Rat_number=23; Rat_sample_name=RYGB_AB_3; RAW_FILE_NAME=KIT3-0-5504_1024032812_82_0_1_1_00_1024038720.wiff SUBJECT_SAMPLE_FACTORS - 1024038734 Tissue:Caecum | Sample treatment:Lean_AB Rat_number=27; Rat_sample_name=Lean_AB_1; RAW_FILE_NAME=KIT3-0-5504_1024032812_94_0_1_1_00_1024038734.wiff SUBJECT_SAMPLE_FACTORS - 1024038749 Tissue:Caecum | Sample treatment:Lean_AB Rat_number=28; Rat_sample_name=Lean_AB_2; RAW_FILE_NAME=KIT3-0-5504_1024032812_11_0_1_1_00_1024038749.wiff SUBJECT_SAMPLE_FACTORS - 1024032725 Tissue:Serum | Sample treatment:Lean_AB Rat_number=28; Rat_sample_name=Lean_AB_2; RAW_FILE_NAME=KIT3-0-5504_1024032812_88_0_1_1_00_1024032725.wiff SUBJECT_SAMPLE_FACTORS - 1024032730 Tissue:Serum | Sample treatment:Lean Rat_number=29; Rat_sample_name=Lean_1; RAW_FILE_NAME=KIT3-0-5504_1024032812_05_0_1_1_00_1024032730.wiff SUBJECT_SAMPLE_FACTORS - 1024038753 Tissue:Caecum | Sample treatment:Lean Rat_number=29; Rat_sample_name=Lean_1; RAW_FILE_NAME=KIT3-0-5504_1024032812_23_0_1_1_00_1024038753.wiff SUBJECT_SAMPLE_FACTORS - 1024035408 Tissue:Serum | Sample treatment:Lean Rat_number=30; Rat_sample_name=Lean_2; RAW_FILE_NAME=KIT3-0-5504_1024032812_17_0_1_1_00_1024035408.wiff SUBJECT_SAMPLE_FACTORS - 1024038768 Tissue:Caecum | Sample treatment:Lean Rat_number=30; Rat_sample_name=Lean_2; RAW_FILE_NAME=KIT3-0-5504_1024032812_35_0_1_1_00_1024038768.wiff SUBJECT_SAMPLE_FACTORS - 1024035412 Tissue:Serum | Sample treatment:Lean_AB Rat_number=31; Rat_sample_name=Lean_AB_3; RAW_FILE_NAME=KIT3-0-5504_1024032812_29_0_1_1_00_1024035412.wiff SUBJECT_SAMPLE_FACTORS - 1024038772 Tissue:Caecum | Sample treatment:Lean_AB Rat_number=31; Rat_sample_name=Lean_AB_3; RAW_FILE_NAME=KIT3-0-5504_1024032812_47_0_1_1_00_1024038772.wiff SUBJECT_SAMPLE_FACTORS - 1024035427 Tissue:Serum | Sample treatment:Lean_AB Rat_number=32; Rat_sample_name=Lean_AB_4; RAW_FILE_NAME=KIT3-0-5504_1024032812_41_0_1_1_00_1024035427.wiff SUBJECT_SAMPLE_FACTORS - 1024038787 Tissue:Caecum | Sample treatment:Lean_AB Rat_number=32; Rat_sample_name=Lean_AB_4; RAW_FILE_NAME=KIT3-0-5504_1024032812_59_0_1_1_00_1024038787.wiff SUBJECT_SAMPLE_FACTORS - 1024035431 Tissue:Serum | Sample treatment:Lean Rat_number=33; Rat_sample_name=Lean_3; RAW_FILE_NAME=KIT3-0-5504_1024032812_53_0_1_1_00_1024035431.wiff SUBJECT_SAMPLE_FACTORS - 1024038791 Tissue:Caecum | Sample treatment:Lean Rat_number=33; Rat_sample_name=Lean_3; RAW_FILE_NAME=KIT3-0-5504_1024032812_71_0_1_1_00_1024038791.wiff SUBJECT_SAMPLE_FACTORS - 1024035451 Tissue:Serum | Sample treatment:Lean Rat_number=34; Rat_sample_name=Lean_4; RAW_FILE_NAME=KIT3-0-5504_1024032812_77_0_1_1_00_1024035451.wiff SUBJECT_SAMPLE_FACTORS - 1024038802 Tissue:Caecum | Sample treatment:Lean Rat_number=34; Rat_sample_name=Lean_4; RAW_FILE_NAME=KIT3-0-5504_1024032812_83_0_1_1_00_1024038802.wiff SUBJECT_SAMPLE_FACTORS - 1024038821 Tissue:Caecum | Sample treatment:RYGB_Donor Rat_number=35; Rat_sample_name=RYGB_Donor_4; RAW_FILE_NAME=KIT3-0-5504_1024032812_12_0_1_1_00_1024038821.wiff SUBJECT_SAMPLE_FACTORS - 1024035465 Tissue:Serum | Sample treatment:RYGB_Donor Rat_number=35; Rat_sample_name=RYGB_Donor_4; RAW_FILE_NAME=KIT3-0-5504_1024032812_89_0_1_1_00_1024035465.wiff SUBJECT_SAMPLE_FACTORS - 1024035470 Tissue:Serum | Sample treatment:RYGB_Donor Rat_number=36; Rat_sample_name=RYGB_Donor_6; RAW_FILE_NAME=KIT3-0-5504_1024032812_06_0_1_1_00_1024035470.wiff SUBJECT_SAMPLE_FACTORS - 1024038836 Tissue:Caecum | Sample treatment:RYGB_Donor Rat_number=36; Rat_sample_name=RYGB_Donor_6; RAW_FILE_NAME=KIT3-0-5504_1024032812_24_0_1_1_00_1024038836.wiff SUBJECT_SAMPLE_FACTORS - 1024035484 Tissue:Serum | Sample treatment:RYGB_Donor Rat_number=37; Rat_sample_name=RYGB_Donor_5; RAW_FILE_NAME=KIT3-0-5504_1024032812_18_0_1_1_00_1024035484.wiff SUBJECT_SAMPLE_FACTORS - 1024032744 Tissue:Caecum | Sample treatment:RYGB_Donor Rat_number=37; Rat_sample_name=RYGB_Donor_5; RAW_FILE_NAME=KIT3-0-5504_1024032812_36_0_1_1_00_1024032744.wiff SUBJECT_SAMPLE_FACTORS - 1024035499 Tissue:Serum | Sample treatment:RYGB_AB Rat_number=39; Rat_sample_name=RYGB_AB_4; RAW_FILE_NAME=KIT3-0-5504_1024032812_30_0_1_1_00_1024035499.wiff SUBJECT_SAMPLE_FACTORS - 1024032759 Tissue:Caecum | Sample treatment:RYGB_AB Rat_number=39; Rat_sample_name=RYGB_AB_4; RAW_FILE_NAME=KIT3-0-5504_1024032812_48_0_1_1_00_1024032759.wiff SUBJECT_SAMPLE_FACTORS - 1024035500 Tissue:Serum | Sample treatment:RYGB_AB Rat_number=40; Rat_sample_name=RYGB_AB_5; RAW_FILE_NAME=KIT3-0-5504_1024032812_42_0_1_1_00_1024035500.wiff SUBJECT_SAMPLE_FACTORS - 1024032763 Tissue:Caecum | Sample treatment:RYGB_AB Rat_number=40; Rat_sample_name=RYGB_AB_5; RAW_FILE_NAME=KIT3-0-5504_1024032812_60_0_1_1_00_1024032763.wiff SUBJECT_SAMPLE_FACTORS - 1024035514 Tissue:Serum | Sample treatment:RYGB_AB Rat_number=41; Rat_sample_name=RYGB_AB_6; RAW_FILE_NAME=KIT3-0-5504_1024032812_54_0_1_1_00_1024035514.wiff SUBJECT_SAMPLE_FACTORS - 1024032778 Tissue:Caecum | Sample treatment:RYGB_AB Rat_number=41; Rat_sample_name=RYGB_AB_6; RAW_FILE_NAME=KIT3-0-5504_1024032812_72_0_1_1_00_1024032778.wiff SUBJECT_SAMPLE_FACTORS - 1024035529 Tissue:Serum | Sample treatment:Lean_AB Rat_number=42; Rat_sample_name=Lean_AB_5; RAW_FILE_NAME=KIT3-0-5504_1024032812_66_0_1_1_00_1024035529.wiff SUBJECT_SAMPLE_FACTORS - 1024032782 Tissue:Caecum | Sample treatment:Lean_AB Rat_number=42; Rat_sample_name=Lean_AB_5; RAW_FILE_NAME=KIT3-0-5504_1024032812_84_0_1_1_00_1024032782.wiff SUBJECT_SAMPLE_FACTORS - 1024035533 Tissue:Serum | Sample treatment:Lean_AB Rat_number=43; Rat_sample_name=Lean_AB_6; RAW_FILE_NAME=KIT3-0-5504_1024032812_78_0_1_1_00_1024035533.wiff SUBJECT_SAMPLE_FACTORS - 1024032797 Tissue:Caecum | Sample treatment:Lean_AB Rat_number=43; Rat_sample_name=Lean_AB_6; RAW_FILE_NAME=KIT3-0-5504_1024032812_96_0_1_1_00_1024032797.wiff SUBJECT_SAMPLE_FACTORS - 1024035548 Tissue:Serum | Sample treatment:DIO_AB Rat_number=47; Rat_sample_name=DIO_AB_7; RAW_FILE_NAME=KIT3-0-5504_1024032812_90_0_1_1_00_1024035548.wiff SUBJECT_SAMPLE_FACTORS - 1024035552 Tissue:Serum | Sample treatment:FMT_lean Rat_number=48; Rat_sample_name=FMT_lean_1; RAW_FILE_NAME=KIT3-0-5504_1024032812_07_0_1_1_00_1024035552.wiff SUBJECT_SAMPLE_FACTORS - 1024035567 Tissue:Serum | Sample treatment:DIO_AB Rat_number=49; Rat_sample_name=DIO_AB_5; RAW_FILE_NAME=KIT3-0-5504_1024032812_19_0_1_1_00_1024035567.wiff SUBJECT_SAMPLE_FACTORS - 1024035571 Tissue:Serum | Sample treatment:Lean Rat_number=54; Rat_sample_name=Lean_5; RAW_FILE_NAME=KIT3-0-5504_1024032812_31_0_1_1_00_1024035571.wiff SUBJECT_SAMPLE_FACTORS - 1024035586 Tissue:Serum | Sample treatment:Lean Rat_number=55; Rat_sample_name=Lean_6; RAW_FILE_NAME=KIT3-0-5504_1024032812_43_0_1_1_00_1024035586.wiff SUBJECT_SAMPLE_FACTORS - 1024041117 Tissue:Serum | Sample treatment:DIO_FMT_RYGB Rat_number=57; Rat_sample_name=DIO_FMT_RYGB_8; RAW_FILE_NAME=KIT3-0-5504_1024032812_55_0_1_1_00_1024041117.wiff SUBJECT_SAMPLE_FACTORS - 1024041121 Tissue:Serum | Sample treatment:FMT_lean Rat_number=58; Rat_sample_name=FMT_lean_2; RAW_FILE_NAME=KIT3-0-5504_1024032812_67_0_1_1_00_1024041121.wiff SUBJECT_SAMPLE_FACTORS - 1024041136 Tissue:Serum | Sample treatment:FMT_lean Rat_number=63; Rat_sample_name=FMT_lean_3; RAW_FILE_NAME=KIT3-0-5504_1024032812_79_0_1_1_00_1024041136.wiff SUBJECT_SAMPLE_FACTORS - 1024041141 Tissue:Serum | Sample treatment:FMT_lean Rat_number=64; Rat_sample_name=FMT_lean_4; RAW_FILE_NAME=KIT3-0-5504_1024032812_91_0_1_1_00_1024041141.wiff SUBJECT_SAMPLE_FACTORS - 1024041155 Tissue:Serum | Sample treatment:DIO Rat_number=67; Rat_sample_name=DIO_11; RAW_FILE_NAME=KIT3-0-5504_1024032812_08_0_1_1_00_1024041155.wiff SUBJECT_SAMPLE_FACTORS - 1024041174 Tissue:Serum | Sample treatment:DIO Rat_number=70; Rat_sample_name=DIO_12; RAW_FILE_NAME=KIT3-0-5504_1024032812_32_0_1_1_00_1024041174.wiff SUBJECT_SAMPLE_FACTORS - 1024041189 Tissue:Serum | Sample treatment:DIO_FMT_RYGB Rat_number=72; Rat_sample_name=DIO_FMT_RYGB_7; RAW_FILE_NAME=KIT3-0-5504_1024032812_44_0_1_1_00_1024041189.wiff SUBJECT_SAMPLE_FACTORS - 1024041193 Tissue:Serum | Sample treatment:FMT_lean Rat_number=73; Rat_sample_name=FMT_lean_5; RAW_FILE_NAME=KIT3-0-5504_1024032812_56_0_1_1_00_1024041193.wiff SUBJECT_SAMPLE_FACTORS - 1024041204 Tissue:Serum | Sample treatment:FMT_lean Rat_number=74; Rat_sample_name=FMT_lean_6; RAW_FILE_NAME=KIT3-0-5504_1024032812_68_0_1_1_00_1024041204.wiff SUBJECT_SAMPLE_FACTORS - 1024032676 Tissue:Serum | Sample treatment:RYGB_AB Rat_number=15a; Rat_sample_name=RYGB_AB_1; RAW_FILE_NAME=KIT3-0-5504_1024032812_28_0_1_1_00_1024032676.wiff SUBJECT_SAMPLE_FACTORS - 1024038690 Tissue:Caecum | Sample treatment:RYGB_AB Rat_number=15a; Rat_sample_name=RYGB_AB_1; RAW_FILE_NAME=KIT3-0-5504_1024032812_46_0_1_1_00_1024038690.wiff SUBJECT_SAMPLE_FACTORS - 1024032695 Tissue:Serum | Sample treatment:RYGB_Donor Rat_number=18b; Rat_sample_name=RYGB_Donor_1; RAW_FILE_NAME=KIT3-0-5504_1024032812_52_0_1_1_00_1024032695.wiff #COLLECTION CO:COLLECTION_SUMMARY Animals were sacrificed, blood and cecum tissue were removed, snap frozen in CO:COLLECTION_SUMMARY liquid nitrogen and stored at -80°C until further analysis CO:SAMPLE_TYPE Cecum #TREATMENT TR:TREATMENT_SUMMARY RYGB surgery was performed on age-matched (8-10-weeks old) male Wistar IGS rats. TR:TREATMENT_SUMMARY After four weeks of recovery, animals were put on an antibiotic cocktail TR:TREATMENT_SUMMARY consisting of ampicillin (1 g/l; Ratiopharm), vancomycin (0.5 g/l; Ratiopharm), TR:TREATMENT_SUMMARY neomycin (1 g/l; Bela-pharm), and metronidazole (1 g/l; CP-Pharma), provided TR:TREATMENT_SUMMARY freshly every day via drinking water. The parallel FMT group received fecal RYGB TR:TREATMENT_SUMMARY microbiota transplantation once per week, but were otherwise handled like DIO TR:TREATMENT_SUMMARY littermates. For fecal transplantation experiments, 100 mg of fresh stool from TR:TREATMENT_SUMMARY bodyweight-stabilized RYGB donors was re-suspended in 1 ml of PBS, homogenized TR:TREATMENT_SUMMARY carefully and administered via oral gavage with 200 μl of the suspension. #SAMPLEPREP SP:SAMPLEPREP_SUMMARY Sample preparation for MetIDQ p180 Kit measurement Solvents: Acetonitril (Merck SP:SAMPLEPREP_SUMMARY KGaA, Darmstadt, Germany hypergrade for LC-MS) Water MiliQ, Extracting agent - SP:SAMPLEPREP_SUMMARY ACN / H2O (1:1) Equipment 4 steel balls size M Eppendorf Tubes 2mL Tissue slicer SP:SAMPLEPREP_SUMMARY (Rettberg, Germany) Centrifuge (Sigma) Work steps Approximately 100mg of caecum SP:SAMPLEPREP_SUMMARY sample and 4 steel balls of size M into each tube. Per mg of sample 5µL of SP:SAMPLEPREP_SUMMARY extracting agent was added. Shake the samples for 10 minutes at 30 Hz in the SP:SAMPLEPREP_SUMMARY tissue slicer and centrifuge for 2 minutes at 14000 rpm. 10 µL supernatant was SP:SAMPLEPREP_SUMMARY used for the targeted analytics. Blood serum samples 10 µL were used for SP:SAMPLEPREP_SUMMARY analysis Kit reparation The analysis was performed using the validated MetIDQ SP:SAMPLEPREP_SUMMARY p180 Kit and described in Siskos et al. [1]. Data processing was carried out SP:SAMPLEPREP_SUMMARY with the provided quantitation method Kit (Biocrates Life Sciences AG, SP:SAMPLEPREP_SUMMARY Innsbruck, Austria). 1 Siskos AP, Jain P, Römisch-Margl W, Bennett M, Achaintre SP:SAMPLEPREP_SUMMARY D, Asad Y, et al. Interlaboratory Reproducibility of a Targeted Metabolomics SP:SAMPLEPREP_SUMMARY Platform for Analysis of Human Serum and Plasma. Anal Chem 2017;89:656-65. #CHROMATOGRAPHY CH:CHROMATOGRAPHY_SUMMARY LC - Instrument Parameters AbsoluteIDQ® p180 Kit (Biocrates Life Science AG, CH:CHROMATOGRAPHY_SUMMARY Innsbruck, Austria) System: UPLC (Waters Acquity, Waters Corporation, Milford, CH:CHROMATOGRAPHY_SUMMARY USA) Column: Agilent, Zorbax Eclipse XDB C18, 3.0 x 100 mm, 3.5 μM, Agilent CH:CHROMATOGRAPHY_SUMMARY Waldbronn, Germany Precolumn: Security Guard, Phenomenex, C18, 4 x 3 mm; CH:CHROMATOGRAPHY_SUMMARY Phenomenex, Aschaffenburg, Germany Materials: Water MiliQ, Methanol (Merck KGaA, CH:CHROMATOGRAPHY_SUMMARY Darmstadt, Germany, hypergrade for LC-MS) Acetonitril (Merck KGaA, Darmstadt, CH:CHROMATOGRAPHY_SUMMARY Germany hypergrade for LC-MS) Ammonium Acetate (Honeywell - Fluka, Seelze, CH:CHROMATOGRAPHY_SUMMARY Germany) Formic Acid (Honeywell, Fluka, Seelze, Germany) Running Solvent: 5mM CH:CHROMATOGRAPHY_SUMMARY ammonium acetat in methanol LC: A: 2mL Formic acid in MilliQ water B: 1mL Formic CH:CHROMATOGRAPHY_SUMMARY Acid in Acetonitrile Gradient Table LC Time (min) Flow Rate (mL/min) %A %B Curve CH:CHROMATOGRAPHY_SUMMARY Initial 0.500 100.0 0.0 Initial 0.50 0.500 100.0 0.0 6 4.00 0.500 30.0 70.0 6 CH:CHROMATOGRAPHY_SUMMARY 5.30 0.500 30.0 70.0 6 5.40 0.500 100.0 0.0 6 7.30 0.500 100.0 0.0 6 CH:CHROMATOGRAPHY_TYPE Reversed phase CH:INSTRUMENT_NAME UPLC (Waters Acquity, Waters Corporation, Milford, USA) CH:COLUMN_NAME Agilent Zorbax Eclipse XDB C18 3.0 x 100mm, 3.5µM, Agilent Waldbronn, Germany CH:METHODS_FILENAME LC_parameters.pdf #ANALYSIS AN:ANALYSIS_TYPE MS AN:ANALYSIS_PROTOCOL_FILE MS_parameters_1.pdf #MS MS:INSTRUMENT_NAME ABI Sciex 5500 QTrap MS:INSTRUMENT_TYPE Triple quadrupole MS:MS_TYPE ESI MS:ION_MODE NEGATIVE MS:MS_COMMENTS Analyst version 1.6 Software from SCIEX. For Validation METIDQ Software version MS:MS_COMMENTS Boron from Biocrates #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS µM MS_METABOLITE_DATA_START Samples 1024033074 1024032986 1024032991 1024035810 1024035839 1024033093 1024033089 1024035843 1024035805 1024032971 1024039011 1024033055 1024035824 1024033060 1024033041 1024035794 1024032952 1024032933 1024039007 1024033104 1024041431 1024038938 1024035761 1024038923 1024032948 1024033017 1024033021 1024041344 1024038942 1024033036 1024041427 1024038919 1024041412 1024041363 1024041408 1024035780 1024038860 1024038841 1024033119 1024039026 1024035858 1024035756 1024041359 1024041446 1024038904 1024038995 1024035775 1024041378 1024038874 1024041311 1024041325 1024041484 1024041382 1024041397 1024038893 1024041330 1024038961 1024038957 1024041451 1024038976 1024038889 1024041465 1024038981 1024041306 1024032555 1024041219 1024032560 1024041223 1024032574 1024041238 1024032589 1024041242 1024032593 1024041257 1024032604 1024041261 1024041276 1024032619 1024041281 1024032623 1024041295 1024032638 1024038651 1024038666 1024032642 1024032657 1024038671 1024032661 1024038685 1024032681 1024038701 1024038715 1024032706 1024032711 1024038720 1024038734 1024038749 1024032725 1024032730 1024038753 1024035408 1024038768 1024035412 1024038772 1024035427 1024038787 1024035431 1024038791 1024035451 1024038802 1024038821 1024035465 1024035470 1024038836 1024035484 1024032744 1024035499 1024032759 1024035500 1024032763 1024035514 1024032778 1024035529 1024032782 1024035533 1024032797 1024035548 1024035552 1024035567 1024035571 1024035586 1024041117 1024041121 1024041136 1024041141 1024041155 1024041174 1024041189 1024041193 1024041204 1024032676 1024038690 1024032695 Factors Tissue:Plasma | Sample treatment:DIO Tissue:Plasma | Sample treatment:RYGB_Donor Tissue:Plasma | Sample treatment:RYGB_AB Tissue:Plasma | Sample treatment:DIO_PF_FMT Tissue:Plasma | Sample treatment:Lean Tissue:Plasma | Sample treatment:DIO Tissue:Plasma | Sample treatment:DIO Tissue:Plasma | Sample treatment:Lean Tissue:Plasma | Sample treatment:DIO_PF_FMT Tissue:Plasma | Sample treatment:RYGB_AB Tissue:Caecum | Sample treatment:Lean Tissue:Plasma | Sample treatment:DIO_FMT_RYGB Tissue:Plasma | Sample treatment:Lean Tissue:Plasma | Sample treatment:DIO Tissue:Plasma | Sample treatment:DIO_FMT_RYGB Tissue:Plasma | Sample treatment:DIO_PF_FMT Tissue:Plasma | Sample treatment:RYGB_Donor Tissue:Plasma | Sample treatment:RYGB_Donor Tissue:Caecum | Sample treatment:Lean Tissue:Plasma | Sample treatment:DIO_PF_FMT Tissue:Caecum | Sample treatment:RYGB_Donor Tissue:Caecum | Sample treatment:DIO_PF_FMT Tissue:Caecum | Sample treatment:FMT_lean Tissue:Caecum | Sample treatment:DIO Tissue:Plasma | Sample treatment:RYGB_Donor Tissue:Plasma | Sample treatment:RYGB_AB Tissue:Plasma | Sample treatment:DIO_FMT_RYGB Tissue:Caecum | Sample treatment:FMT_lean Tissue:Caecum | Sample treatment:DIO_PF_FMT Tissue:Plasma | Sample treatment:DIO_FMT_RYGB Tissue:Caecum | Sample treatment:RYGB_Donor Tissue:Caecum | Sample treatment:DIO Tissue:Caecum | Sample treatment:RYGB_Donor Tissue:Caecum | Sample treatment:DIO Tissue:Caecum | Sample treatment:FMT_lean Tissue:Plasma | Sample treatment:DIO_PF_FMT Tissue:Caecum | Sample treatment:DIO_FMT_RYGB Tissue:Caecum | Sample treatment:DIO_FMT_RYGB Tissue:Plasma | Sample treatment:DIO_PF_FMT Tissue:Caecum | Sample treatment:Lean Tissue:Plasma | Sample treatment:Lean Tissue:Caecum | Sample treatment:DIO_AB Tissue:Caecum | Sample treatment:FMT_lean Tissue:Caecum | Sample treatment:RYGB_AB Tissue:Caecum | Sample treatment:DIO Tissue:Caecum | Sample treatment:Lean Tissue:Caecum | Sample treatment:DIO_AB Tissue:Caecum | Sample treatment:DIO Tissue:Caecum | Sample treatment:DIO_FMT_RYGB Tissue:Caecum | Sample treatment:Lean Tissue:Caecum | Sample treatment:DIO_FMT_RYGB Tissue:Caecum | Sample treatment:RYGB_AB Tissue:Caecum | Sample treatment:DIO_FMT_RYGB Tissue:Caecum | Sample treatment:FMT_lean Tissue:Caecum | Sample treatment:DIO Tissue:Caecum | Sample treatment:FMT_lean Tissue:Caecum | Sample treatment:DIO_PF_FMT Tissue:Caecum | Sample treatment:DIO_PF_FMT Tissue:Caecum | Sample treatment:RYGB_Donor Tissue:Caecum | Sample treatment:DIO_PF_FMT Tissue:Caecum | Sample treatment:DIO_FMT_RYGB Tissue:Caecum | Sample treatment:RYGB_AB Tissue:Caecum | Sample treatment:DIO_PF_FMT Tissue:Caecum | Sample treatment:Lean Tissue:Serum | Sample treatment:DIO Tissue:Caecum | Sample treatment:DIO Tissue:Serum | Sample treatment:DIO_AB Tissue:Caecum | Sample treatment:DIO_AB Tissue:Serum | Sample treatment:DIO_AB Tissue:Caecum | Sample treatment:DIO_AB Tissue:Serum | Sample treatment:DIO_AB Tissue:Caecum | Sample treatment:DIO_AB Tissue:Serum | Sample treatment:DIO_FMT_RYGB Tissue:Caecum | Sample treatment:DIO_FMT_RYGB Tissue:Serum | Sample treatment:DIO_AB Tissue:Caecum | Sample treatment:DIO_AB Tissue:Caecum | Sample treatment:DIO Tissue:Serum | Sample treatment:DIO_FMT_RYGB Tissue:Caecum | Sample treatment:DIO_FMT_RYGB Tissue:Serum | Sample treatment:DIO Tissue:Caecum | Sample treatment:DIO Tissue:Serum | Sample treatment:DIO Tissue:Caecum | Sample treatment:DIO Tissue:Caecum | Sample treatment:DIO_FMT_RYGB Tissue:Serum | Sample treatment:DIO_FMT_RYGB Tissue:Serum | Sample treatment:DIO_FMT_RYGB Tissue:Caecum | Sample treatment:DIO_FMT_RYGB Tissue:Serum | Sample treatment:RYGB_AB Tissue:Caecum | Sample treatment:RYGB_AB Tissue:Serum | Sample treatment:RYGB_Donor Tissue:Caecum | Sample treatment:RYGB_Donor Tissue:Caecum | Sample treatment:RYGB_Donor Tissue:Serum | Sample treatment:RYGB_Donor Tissue:Serum | Sample treatment:RYGB_AB Tissue:Caecum | Sample treatment:RYGB_AB Tissue:Caecum | Sample treatment:Lean_AB Tissue:Caecum | Sample treatment:Lean_AB Tissue:Serum | Sample treatment:Lean_AB Tissue:Serum | Sample treatment:Lean Tissue:Caecum | Sample treatment:Lean Tissue:Serum | Sample treatment:Lean Tissue:Caecum | Sample treatment:Lean Tissue:Serum | Sample treatment:Lean_AB Tissue:Caecum | Sample treatment:Lean_AB Tissue:Serum | Sample treatment:Lean_AB Tissue:Caecum | Sample treatment:Lean_AB Tissue:Serum | Sample treatment:Lean Tissue:Caecum | Sample treatment:Lean Tissue:Serum | Sample treatment:Lean Tissue:Caecum | Sample treatment:Lean Tissue:Caecum | Sample treatment:RYGB_Donor Tissue:Serum | Sample treatment:RYGB_Donor Tissue:Serum | Sample treatment:RYGB_Donor Tissue:Caecum | Sample treatment:RYGB_Donor Tissue:Serum | Sample treatment:RYGB_Donor Tissue:Caecum | Sample treatment:RYGB_Donor Tissue:Serum | Sample treatment:RYGB_AB Tissue:Caecum | Sample treatment:RYGB_AB Tissue:Serum | Sample treatment:RYGB_AB Tissue:Caecum | Sample treatment:RYGB_AB Tissue:Serum | Sample treatment:RYGB_AB Tissue:Caecum | Sample treatment:RYGB_AB Tissue:Serum | Sample treatment:Lean_AB Tissue:Caecum | Sample treatment:Lean_AB Tissue:Serum | Sample treatment:Lean_AB Tissue:Caecum | Sample treatment:Lean_AB Tissue:Serum | Sample treatment:DIO_AB Tissue:Serum | Sample treatment:FMT_lean Tissue:Serum | Sample treatment:DIO_AB Tissue:Serum | Sample treatment:Lean Tissue:Serum | Sample treatment:Lean Tissue:Serum | Sample treatment:DIO_FMT_RYGB Tissue:Serum | Sample treatment:FMT_lean Tissue:Serum | Sample treatment:FMT_lean Tissue:Serum | Sample treatment:FMT_lean Tissue:Serum | Sample treatment:DIO Tissue:Serum | Sample treatment:DIO Tissue:Serum | Sample treatment:DIO_FMT_RYGB Tissue:Serum | Sample treatment:FMT_lean Tissue:Serum | Sample treatment:FMT_lean Tissue:Serum | Sample treatment:RYGB_AB Tissue:Caecum | Sample treatment:RYGB_AB Tissue:Serum | Sample treatment:RYGB_Donor Hexose 15682 21228 20658 19052 17455 22409 19385 14004 19894 25118 1186 18673 16734 19073 11844 30944 16641 20836 595 20723 111 988 95.9 244 23473 28039 24142 532 1121 13448 70 703 354 61.5 1448 23398 879 192 12796 633 14966 199 194 37.3 431 558 36.1 168 1291 229 296 311 318 154 990 457 597 2195 305 804 952 142 1036 503 29734 59.9 18991 109 31591 45.2 14437 76.1 30799 30.6 47060 59.4 57.7 37238 42.4 21335 32.3 31384 25.4 27.1 27958 15492 34.2 25476 22.2 24069 20.2 32.2 15832 11362 34.7 25.1 21.2 17475 29257 28.4 9372 33.2 25777 27.4 33901 20.5 25337 20.8 15797 26.9 2399 19449 36179 281 25362 43.3 19256 177 43698 57 29095 213 5844 333 25523 16821 19784 10682 21157 9258 27619 24657 16931 12314 31271 19582 2529 10491 32111 21981 19473 77.4 37092 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name KEGG Hexose C00031, C00159, C00221, C00247, C00738, C00267, C00764, C00795, C00936, C00962, C00984, C01452, C01487, C01582, C01719, C01825, C06465, C06466, C06467, C06468, C10906, C15923 METABOLITES_END #END