#METABOLOMICS WORKBENCH sasakiwustl_20220322_152739 DATATRACK_ID:3135 STUDY_ID:ST002117 ANALYSIS_ID:AN003465 PROJECT_ID:PR001342
VERSION             	1
CREATED_ON             	March 25, 2022, 1:20 pm
#PROJECT
PR:PROJECT_TITLE                 	Microbial Toll/interleukin-1 domain metabolite study.
PR:PROJECT_SUMMARY               	The Characterization of NADase activities of 151 bacterial TIRs.
PR:INSTITUTE                     	Washington University, St. Louis
PR:LAST_NAME                     	Yo
PR:FIRST_NAME                    	Sasaki
PR:ADDRESS                       	4515 McKinley Ave St.Louis MO
PR:EMAIL                         	sasaki@wustl.edu
PR:PHONE                         	314-362-4653
#STUDY
ST:STUDY_TITLE                   	Microbial Toll/interleukin-1 domain NADase activities
ST:STUDY_SUMMARY                 	Recent work indicates that Toll/Interleukin-1 receptor (TIR) domain-containing
ST:STUDY_SUMMARY                 	proteins degrade NAD. Given increasing attention to how the gut microbiome
ST:STUDY_SUMMARY                 	contributes to NAD metabolism and healthy growth, we examined the representation
ST:STUDY_SUMMARY                 	and expressed NADase activities of its TIR domains. The NADase activities of 151
ST:STUDY_SUMMARY                 	bacterial TIRs were characterized in vitro. Gut bacterial strains representing
ST:STUDY_SUMMARY                 	the diversity of TIRs observed in the microbiome, and activities observed in
ST:STUDY_SUMMARY                 	vitro, were introduced into germ-free mice fed defined diets. Mass spectrometry
ST:STUDY_SUMMARY                 	of cecal metabolites disclosed that a product of TIR NADase activity, variant
ST:STUDY_SUMMARY                 	cyclic-ADPR-x, distinguished colonized from germ-free animals. Mass spectrometry
ST:STUDY_SUMMARY                 	and microbial RNA-Seq of gnotobiotic mice colonized with one, two and all
ST:STUDY_SUMMARY                 	members of this bacterial consortium, identified Bacteroides xylanisolvens as
ST:STUDY_SUMMARY                 	the principal in vivo source of v-cADPR-x. Guided by bioinformatic analyses of
ST:STUDY_SUMMARY                 	biochemically validated TIR domains, we determined that compared to age-matched
ST:STUDY_SUMMARY                 	healthy Bangladeshi children, those with acute malnutrition had significantly
ST:STUDY_SUMMARY                 	lower fecal levels of TIRs known or predicted to generate v-cADPR-x, and of this
ST:STUDY_SUMMARY                 	metabolite. These results indicate that v-cADPR-x may be an informative
ST:STUDY_SUMMARY                 	biomarker of healthy gut microbiome development.
ST:INSTITUTE                     	Washington University, St. Louis
ST:LAST_NAME                     	Sasaki
ST:FIRST_NAME                    	Yo
ST:ADDRESS                       	4515 McKinley Ave St.Louis MO
ST:EMAIL                         	sasaki@wustl.edu
ST:PHONE                         	314-362-4653
#SUBJECT
SU:SUBJECT_TYPE                  	Bacteria
SU:SUBJECT_SPECIES               	Bacteroides xylanisolvens
#FACTORS
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data
SUBJECT_SAMPLE_FACTORS           	-	m09a-S5A	Cage:1 | Microbiota:Germfree | Diet:NAD-precursor sufficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	data table name=S5A; RAW_FILE_NAME=07 26 2021 Jimmy feces-14
SUBJECT_SAMPLE_FACTORS           	-	m01b-S5A	Cage:1 | Microbiota:Germfree | Diet:NAD-precursor sufficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	data table name=S5A; RAW_FILE_NAME=07 26 2021 Jimmy feces-15
SUBJECT_SAMPLE_FACTORS           	-	m07b-S5A	Cage:1 | Microbiota:Germfree | Diet:NAD-precursor sufficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	data table name=S5A; RAW_FILE_NAME=07 26 2021 Jimmy feces-16
SUBJECT_SAMPLE_FACTORS           	-	m08a-S5A	Cage:1 | Microbiota:Germfree | Diet:NAD-precursor sufficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	data table name=S5A; RAW_FILE_NAME=07 26 2021 Jimmy feces-17
SUBJECT_SAMPLE_FACTORS           	-	m11b-S5A	Cage:2 | Microbiota:Germfree | Diet:NAD-precursor sufficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	data table name=S5A; RAW_FILE_NAME=07 26 2021 Jimmy feces-18
SUBJECT_SAMPLE_FACTORS           	-	m14a-S5A	Cage:2 | Microbiota:Germfree | Diet:NAD-precursor sufficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	data table name=S5A; RAW_FILE_NAME=07 26 2021 Jimmy feces-19
SUBJECT_SAMPLE_FACTORS           	-	m17a-S5A	Cage:2 | Microbiota:Germfree | Diet:NAD-precursor sufficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	data table name=S5A; RAW_FILE_NAME=07 26 2021 Jimmy feces-20
SUBJECT_SAMPLE_FACTORS           	-	m13b-S5A	Cage:2 | Microbiota:Germfree | Diet:NAD-precursor sufficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	data table name=S5A; RAW_FILE_NAME=07 26 2021 Jimmy feces-21
SUBJECT_SAMPLE_FACTORS           	-	m16a-S5A	Cage:3 | Microbiota:Germfree | Diet:NAD-precursor deficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	data table name=S5A; RAW_FILE_NAME=07 26 2021 Jimmy feces-22
SUBJECT_SAMPLE_FACTORS           	-	m05b-S5A	Cage:3 | Microbiota:Germfree | Diet:NAD-precursor deficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	data table name=S5A; RAW_FILE_NAME=07 26 2021 Jimmy feces-23
SUBJECT_SAMPLE_FACTORS           	-	m02a-S5A	Cage:3 | Microbiota:Germfree | Diet:NAD-precursor deficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	data table name=S5A; RAW_FILE_NAME=07 26 2021 Jimmy feces-24
SUBJECT_SAMPLE_FACTORS           	-	m14b-S5A	Cage:3 | Microbiota:Germfree | Diet:NAD-precursor deficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	data table name=S5A; RAW_FILE_NAME=07 26 2021 Jimmy feces-25
SUBJECT_SAMPLE_FACTORS           	-	m02b-S5A	Cage:4 | Microbiota:Germfree | Diet:NAD-precursor deficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	data table name=S5A; RAW_FILE_NAME=07 26 2021 Jimmy feces-26
SUBJECT_SAMPLE_FACTORS           	-	m08b-S5A	Cage:4 | Microbiota:Germfree | Diet:NAD-precursor deficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	data table name=S5A; RAW_FILE_NAME=07 26 2021 Jimmy feces-27
SUBJECT_SAMPLE_FACTORS           	-	m03b-S5A	Cage:4 | Microbiota:Germfree | Diet:NAD-precursor deficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	data table name=S5A; RAW_FILE_NAME=07 26 2021 Jimmy feces-28
SUBJECT_SAMPLE_FACTORS           	-	m05a-S5A	Cage:4 | Microbiota:Germfree | Diet:NAD-precursor deficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	data table name=S5A; RAW_FILE_NAME=07 26 2021 Jimmy feces-29
SUBJECT_SAMPLE_FACTORS           	-	m15a-S5A	Cage:1 | Microbiota:26-member | Diet:NAD-precursor sufficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	data table name=S5A; RAW_FILE_NAME=07 26 2021 Jimmy feces-30
SUBJECT_SAMPLE_FACTORS           	-	m11a-S5A	Cage:1 | Microbiota:26-member | Diet:NAD-precursor sufficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	data table name=S5A; RAW_FILE_NAME=07 26 2021 Jimmy feces-31
SUBJECT_SAMPLE_FACTORS           	-	m13a-S5A	Cage:1 | Microbiota:26-member | Diet:NAD-precursor sufficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	data table name=S5A; RAW_FILE_NAME=07 26 2021 Jimmy feces-32
SUBJECT_SAMPLE_FACTORS           	-	m07a-S5A	Cage:1 | Microbiota:26-member | Diet:NAD-precursor sufficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	data table name=S5A; RAW_FILE_NAME=07 26 2021 Jimmy feces-33
SUBJECT_SAMPLE_FACTORS           	-	m06b-S5A	Cage:2 | Microbiota:26-member | Diet:NAD-precursor sufficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	data table name=S5A; RAW_FILE_NAME=07 26 2021 Jimmy feces-34
SUBJECT_SAMPLE_FACTORS           	-	m10b-S5A	Cage:2 | Microbiota:26-member | Diet:NAD-precursor sufficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	data table name=S5A; RAW_FILE_NAME=07 26 2021 Jimmy feces-35
SUBJECT_SAMPLE_FACTORS           	-	m18a-S5A	Cage:2 | Microbiota:26-member | Diet:NAD-precursor sufficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	data table name=S5A; RAW_FILE_NAME=07 26 2021 Jimmy feces-36
SUBJECT_SAMPLE_FACTORS           	-	m01a-S5A	Cage:2 | Microbiota:26-member | Diet:NAD-precursor sufficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	data table name=S5A; RAW_FILE_NAME=07 26 2021 Jimmy feces-37
SUBJECT_SAMPLE_FACTORS           	-	m12b-S5A	Cage:3 | Microbiota:26-member | Diet:NAD-precursor deficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	data table name=S5A; RAW_FILE_NAME=07 26 2021 Jimmy feces-38
SUBJECT_SAMPLE_FACTORS           	-	m06a-S5A	Cage:3 | Microbiota:26-member | Diet:NAD-precursor deficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	data table name=S5A; RAW_FILE_NAME=07 26 2021 Jimmy feces-39
SUBJECT_SAMPLE_FACTORS           	-	m04b-S5A	Cage:3 | Microbiota:26-member | Diet:NAD-precursor deficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	data table name=S5A; RAW_FILE_NAME=07 26 2021 Jimmy feces-40
SUBJECT_SAMPLE_FACTORS           	-	m10a-S5A	Cage:3 | Microbiota:26-member | Diet:NAD-precursor deficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	data table name=S5A; RAW_FILE_NAME=07 26 2021 Jimmy feces-41
SUBJECT_SAMPLE_FACTORS           	-	m09b-S5A	Cage:4 | Microbiota:26-member | Diet:NAD-precursor deficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	data table name=S5A; RAW_FILE_NAME=07 26 2021 Jimmy feces-42
SUBJECT_SAMPLE_FACTORS           	-	m04a-S5A	Cage:4 | Microbiota:26-member | Diet:NAD-precursor deficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	data table name=S5A; RAW_FILE_NAME=07 26 2021 Jimmy feces-43
SUBJECT_SAMPLE_FACTORS           	-	m03a-S5A	Cage:4 | Microbiota:26-member | Diet:NAD-precursor deficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	data table name=S5A; RAW_FILE_NAME=07 26 2021 Jimmy feces-44
SUBJECT_SAMPLE_FACTORS           	-	m12a-S5A	Cage:4 | Microbiota:26-member | Diet:NAD-precursor deficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	data table name=S5A; RAW_FILE_NAME=07 26 2021 Jimmy feces-45
SUBJECT_SAMPLE_FACTORS           	-	m05b	Cage:1 | Microbiota:Btheta | Diet:NAD-precursor deficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	data table name=S5B; RAW_FILE_NAME=Jimmy contents 11 16 2018-3
SUBJECT_SAMPLE_FACTORS           	-	m14a	Cage:1 | Microbiota:Btheta | Diet:NAD-precursor deficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	data table name=S5B; RAW_FILE_NAME=Jimmy contents 11 16 2018-12
SUBJECT_SAMPLE_FACTORS           	-	m13a	Cage:1 | Microbiota:Btheta | Diet:NAD-precursor deficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	data table name=S5B; RAW_FILE_NAME=Jimmy contents 11 16 2018-21
SUBJECT_SAMPLE_FACTORS           	-	m15a	Cage:2 | Microbiota:Btheta | Diet:NAD-precursor deficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	data table name=S5B; RAW_FILE_NAME=Jimmy contents 11 16 2018-30
SUBJECT_SAMPLE_FACTORS           	-	m12a	Cage:2 | Microbiota:Btheta | Diet:NAD-precursor deficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	data table name=S5B; RAW_FILE_NAME=Jimmy contents 11 16 2018-39
SUBJECT_SAMPLE_FACTORS           	-	m04c	Cage:2 | Microbiota:Btheta | Diet:NAD-precursor deficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	data table name=S5B; RAW_FILE_NAME=Jimmy contents 11 16 2018-48
SUBJECT_SAMPLE_FACTORS           	-	m10a	Cage:3 | Microbiota:Btheta | Diet:NA-supplementeded | Age (months):NA | WLZ:NA | Helath phenotype:NA	data table name=S5B; RAW_FILE_NAME=Jimmy contents 11 16 2018-4
SUBJECT_SAMPLE_FACTORS           	-	m02c	Cage:3 | Microbiota:Btheta | Diet:NA-supplementeded | Age (months):NA | WLZ:NA | Helath phenotype:NA	data table name=S5B; RAW_FILE_NAME=Jimmy contents 11 16 2018-13
SUBJECT_SAMPLE_FACTORS           	-	m03b	Cage:3 | Microbiota:Btheta | Diet:NA-supplementeded | Age (months):NA | WLZ:NA | Helath phenotype:NA	data table name=S5B; RAW_FILE_NAME=Jimmy contents 11 16 2018-22
SUBJECT_SAMPLE_FACTORS           	-	m17a	Cage:4 | Microbiota:Btheta | Diet:NA-supplementeded | Age (months):NA | WLZ:NA | Helath phenotype:NA	data table name=S5B; RAW_FILE_NAME=Jimmy contents 11 16 2018-31
SUBJECT_SAMPLE_FACTORS           	-	m18a	Cage:4 | Microbiota:Btheta | Diet:NA-supplementeded | Age (months):NA | WLZ:NA | Helath phenotype:NA	data table name=S5B; RAW_FILE_NAME=Jimmy contents 11 16 2018-40
SUBJECT_SAMPLE_FACTORS           	-	m16a	Cage:4 | Microbiota:Btheta | Diet:NA-supplementeded | Age (months):NA | WLZ:NA | Helath phenotype:NA	data table name=S5B; RAW_FILE_NAME=Jimmy contents 11 16 2018-41
SUBJECT_SAMPLE_FACTORS           	-	m03c	Cage:1 | Microbiota:Bxylan | Diet:NAD-precursor deficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	data table name=S5B; RAW_FILE_NAME=Jimmy contents 11 16 2018-5
SUBJECT_SAMPLE_FACTORS           	-	m01c	Cage:1 | Microbiota:Bxylan | Diet:NAD-precursor deficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	data table name=S5B; RAW_FILE_NAME=Jimmy contents 11 16 2018-14
SUBJECT_SAMPLE_FACTORS           	-	m07a	Cage:1 | Microbiota:Bxylan | Diet:NAD-precursor deficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	data table name=S5B; RAW_FILE_NAME=Jimmy contents 11 16 2018-23
SUBJECT_SAMPLE_FACTORS           	-	m05c	Cage:2 | Microbiota:Bxylan | Diet:NAD-precursor deficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	data table name=S5B; RAW_FILE_NAME=Jimmy contents 11 16 2018-32
SUBJECT_SAMPLE_FACTORS           	-	m04d	Cage:2 | Microbiota:Bxylan | Diet:NAD-precursor deficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	data table name=S5B; RAW_FILE_NAME=Jimmy contents 11 16 2018-33
SUBJECT_SAMPLE_FACTORS           	-	m08c	Cage:2 | Microbiota:Bxylan | Diet:NAD-precursor deficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	data table name=S5B; RAW_FILE_NAME=Jimmy contents 11 16 2018-42
SUBJECT_SAMPLE_FACTORS           	-	m13b	Cage:3 | Microbiota:Bxylan | Diet:NA-supplementeded | Age (months):NA | WLZ:NA | Helath phenotype:NA	data table name=S5B; RAW_FILE_NAME=Jimmy contents 11 16 2018-6
SUBJECT_SAMPLE_FACTORS           	-	m09b	Cage:3 | Microbiota:Bxylan | Diet:NA-supplementeded | Age (months):NA | WLZ:NA | Helath phenotype:NA	data table name=S5B; RAW_FILE_NAME=Jimmy contents 11 16 2018-15
SUBJECT_SAMPLE_FACTORS           	-	m10b	Cage:3 | Microbiota:Bxylan | Diet:NA-supplementeded | Age (months):NA | WLZ:NA | Helath phenotype:NA	data table name=S5B; RAW_FILE_NAME=Jimmy contents 11 16 2018-24
SUBJECT_SAMPLE_FACTORS           	-	m06b	Cage:4 | Microbiota:Bxylan | Diet:NA-supplementeded | Age (months):NA | WLZ:NA | Helath phenotype:NA	data table name=S5B; RAW_FILE_NAME=Jimmy contents 11 16 2018-25
SUBJECT_SAMPLE_FACTORS           	-	m12b	Cage:4 | Microbiota:Bxylan | Diet:NA-supplementeded | Age (months):NA | WLZ:NA | Helath phenotype:NA	data table name=S5B; RAW_FILE_NAME=Jimmy contents 11 16 2018-34
SUBJECT_SAMPLE_FACTORS           	-	m07b	Cage:4 | Microbiota:Bxylan | Diet:NA-supplementeded | Age (months):NA | WLZ:NA | Helath phenotype:NA	data table name=S5B; RAW_FILE_NAME=Jimmy contents 11 16 2018-43
SUBJECT_SAMPLE_FACTORS           	-	m01a	Cage:1 | Microbiota:Germfree | Diet:NAD-precursor deficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	data table name=S5B; RAW_FILE_NAME=Jimmy contents 11 16 2018-1
SUBJECT_SAMPLE_FACTORS           	-	m02a	Cage:1 | Microbiota:Germfree | Diet:NAD-precursor deficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	data table name=S5B; RAW_FILE_NAME=Jimmy contents 11 16 2018-10
SUBJECT_SAMPLE_FACTORS           	-	m08a	Cage:1 | Microbiota:Germfree | Diet:NAD-precursor deficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	data table name=S5B; RAW_FILE_NAME=Jimmy contents 11 16 2018-19
SUBJECT_SAMPLE_FACTORS           	-	m03a	Cage:2 | Microbiota:Germfree | Diet:NAD-precursor deficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	data table name=S5B; RAW_FILE_NAME=Jimmy contents 11 16 2018-28
SUBJECT_SAMPLE_FACTORS           	-	m05a	Cage:2 | Microbiota:Germfree | Diet:NAD-precursor deficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	data table name=S5B; RAW_FILE_NAME=Jimmy contents 11 16 2018-37
SUBJECT_SAMPLE_FACTORS           	-	m04a	Cage:2 | Microbiota:Germfree | Diet:NAD-precursor deficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	data table name=S5B; RAW_FILE_NAME=Jimmy contents 11 16 2018-46
SUBJECT_SAMPLE_FACTORS           	-	m06a	Cage:3 | Microbiota:Germfree | Diet:NA-supplementeded | Age (months):NA | WLZ:NA | Helath phenotype:NA	data table name=S5B; RAW_FILE_NAME=Jimmy contents 11 16 2018-2
SUBJECT_SAMPLE_FACTORS           	-	m09a	Cage:3 | Microbiota:Germfree | Diet:NA-supplementeded | Age (months):NA | WLZ:NA | Helath phenotype:NA	data table name=S5B; RAW_FILE_NAME=Jimmy contents 11 16 2018-11
SUBJECT_SAMPLE_FACTORS           	-	m04b	Cage:3 | Microbiota:Germfree | Diet:NA-supplementeded | Age (months):NA | WLZ:NA | Helath phenotype:NA	data table name=S5B; RAW_FILE_NAME=Jimmy contents 11 16 2018-20
SUBJECT_SAMPLE_FACTORS           	-	m08b	Cage:4 | Microbiota:Germfree | Diet:NA-supplementeded | Age (months):NA | WLZ:NA | Helath phenotype:NA	data table name=S5B; RAW_FILE_NAME=Jimmy contents 11 16 2018-29
SUBJECT_SAMPLE_FACTORS           	-	m01b	Cage:4 | Microbiota:Germfree | Diet:NA-supplementeded | Age (months):NA | WLZ:NA | Helath phenotype:NA	data table name=S5B; RAW_FILE_NAME=Jimmy contents 11 16 2018-38
SUBJECT_SAMPLE_FACTORS           	-	m02b	Cage:4 | Microbiota:Germfree | Diet:NA-supplementeded | Age (months):NA | WLZ:NA | Helath phenotype:NA	data table name=S5B; RAW_FILE_NAME=Jimmy contents 11 16 2018-47
SUBJECT_SAMPLE_FACTORS           	-	m06c	Cage:1 | Microbiota:26-member | Diet:NAD-precursor deficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	data table name=S5B; RAW_FILE_NAME=Jimmy contents 11 16 2018-8
SUBJECT_SAMPLE_FACTORS           	-	m02d	Cage:1 | Microbiota:26-member | Diet:NAD-precursor deficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	data table name=S5B; RAW_FILE_NAME=Jimmy contents 11 16 2018-9
SUBJECT_SAMPLE_FACTORS           	-	m18b	Cage:1 | Microbiota:26-member | Diet:NAD-precursor deficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	data table name=S5B; RAW_FILE_NAME=Jimmy contents 11 16 2018-18
SUBJECT_SAMPLE_FACTORS           	-	m01d	Cage:2 | Microbiota:26-member | Diet:NAD-precursor deficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	data table name=S5B; RAW_FILE_NAME=Jimmy contents 11 16 2018-27
SUBJECT_SAMPLE_FACTORS           	-	m07c	Cage:2 | Microbiota:26-member | Diet:NAD-precursor deficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	data table name=S5B; RAW_FILE_NAME=Jimmy contents 11 16 2018-36
SUBJECT_SAMPLE_FACTORS           	-	m11b	Cage:2 | Microbiota:26-member | Diet:NAD-precursor deficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	data table name=S5B; RAW_FILE_NAME=Jimmy contents 11 16 2018-45
SUBJECT_SAMPLE_FACTORS           	-	m11a	Cage:1 | Microbiota:BthetaBxylan | Diet:NAD-precursor deficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	data table name=S5B; RAW_FILE_NAME=Jimmy contents 11 16 2018-7
SUBJECT_SAMPLE_FACTORS           	-	m14b	Cage:1 | Microbiota:BthetaBxylan | Diet:NAD-precursor deficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	data table name=S5B; RAW_FILE_NAME=Jimmy contents 11 16 2018-16
SUBJECT_SAMPLE_FACTORS           	-	m17b	Cage:1 | Microbiota:BthetaBxylan | Diet:NAD-precursor deficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	data table name=S5B; RAW_FILE_NAME=Jimmy contents 11 16 2018-17
SUBJECT_SAMPLE_FACTORS           	-	m16b	Cage:2 | Microbiota:BthetaBxylan | Diet:NAD-precursor deficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	data table name=S5B; RAW_FILE_NAME=Jimmy contents 11 16 2018-26
SUBJECT_SAMPLE_FACTORS           	-	m15b	Cage:2 | Microbiota:BthetaBxylan | Diet:NAD-precursor deficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	data table name=S5B; RAW_FILE_NAME=Jimmy contents 11 16 2018-35
SUBJECT_SAMPLE_FACTORS           	-	m03d	Cage:2 | Microbiota:BthetaBxylan | Diet:NAD-precursor deficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	data table name=S5B; RAW_FILE_NAME=Jimmy contents 11 16 2018-44
SUBJECT_SAMPLE_FACTORS           	-	BG1C0462	Cage:NA | Microbiota:NA | Diet:NA | Age (months):8 | WLZ:-0.43 | Helath phenotype:Healthy	data table name=S5C; RAW_FILE_NAME=2021 08 03 Jimmy fecal sample-4
SUBJECT_SAMPLE_FACTORS           	-	BG1C0466-12	Cage:NA | Microbiota:NA | Diet:NA | Age (months):12 | WLZ:0.2 | Helath phenotype:Healthy	data table name=S5C; RAW_FILE_NAME=2022 08 03 Jimmy fecal sample-8
SUBJECT_SAMPLE_FACTORS           	-	BG1C0466-13	Cage:NA | Microbiota:NA | Diet:NA | Age (months):13 | WLZ:-0.3 | Helath phenotype:Healthy	data table name=S5C; RAW_FILE_NAME=2023 08 03 Jimmy fecal sample-12
SUBJECT_SAMPLE_FACTORS           	-	BG1C0473-11	Cage:NA | Microbiota:NA | Diet:NA | Age (months):11 | WLZ:-0.82 | Helath phenotype:Healthy	data table name=S5C; RAW_FILE_NAME=2024 08 03 Jimmy fecal sample-17
SUBJECT_SAMPLE_FACTORS           	-	BG1C0473-13	Cage:NA | Microbiota:NA | Diet:NA | Age (months):13 | WLZ:-0.93 | Helath phenotype:Healthy	data table name=S5C; RAW_FILE_NAME=2025 08 03 Jimmy fecal sample-21
SUBJECT_SAMPLE_FACTORS           	-	Bgsng8064	Cage:NA | Microbiota:NA | Diet:NA | Age (months):15 | WLZ:0.6 | Helath phenotype:Healthy	data table name=S5C; RAW_FILE_NAME=2026 08 03 Jimmy fecal sample-25
SUBJECT_SAMPLE_FACTORS           	-	Bgsng8119	Cage:NA | Microbiota:NA | Diet:NA | Age (months):25 | WLZ:1.7 | Helath phenotype:Healthy	data table name=S5C; RAW_FILE_NAME=2027 08 03 Jimmy fecal sample-30
SUBJECT_SAMPLE_FACTORS           	-	Bgsng8153	Cage:NA | Microbiota:NA | Diet:NA | Age (months):6 | WLZ:1.58 | Helath phenotype:Healthy	data table name=S5C; RAW_FILE_NAME=2028 08 03 Jimmy fecal sample-33
SUBJECT_SAMPLE_FACTORS           	-	Bgsng8169-28	Cage:NA | Microbiota:NA | Diet:NA | Age (months):28 | WLZ:- | Helath phenotype:Healthy	data table name=S5C; RAW_FILE_NAME=2025 08 03 Jimmy fecal sample-5
SUBJECT_SAMPLE_FACTORS           	-	Bgsng8169-30	Cage:NA | Microbiota:NA | Diet:NA | Age (months):30 | WLZ:1.77 | Helath phenotype:Healthy	data table name=S5C; RAW_FILE_NAME=2026 08 03 Jimmy fecal sample-9
SUBJECT_SAMPLE_FACTORS           	-	PS.004	Cage:NA | Microbiota:NA | Diet:NA | Age (months):21.9 | WLZ:-2.03 | Helath phenotype:Malnourished	data table name=S5C; RAW_FILE_NAME=2027 08 03 Jimmy fecal sample-13
SUBJECT_SAMPLE_FACTORS           	-	PS.011	Cage:NA | Microbiota:NA | Diet:NA | Age (months):9.9 | WLZ:-5.54 | Helath phenotype:Malnourished	data table name=S5C; RAW_FILE_NAME=2028 08 03 Jimmy fecal sample-18
SUBJECT_SAMPLE_FACTORS           	-	PS.057-10.4	Cage:NA | Microbiota:NA | Diet:NA | Age (months):10.4 | WLZ:-3.8 | Helath phenotype:Malnourished	data table name=S5C; RAW_FILE_NAME=2029 08 03 Jimmy fecal sample-26
SUBJECT_SAMPLE_FACTORS           	-	PS.057-10.6	Cage:NA | Microbiota:NA | Diet:NA | Age (months):10.6 | WLZ:-2.81 | Helath phenotype:Malnourished	data table name=S5C; RAW_FILE_NAME=2030 08 03 Jimmy fecal sample-31
SUBJECT_SAMPLE_FACTORS           	-	PS.079	Cage:NA | Microbiota:NA | Diet:NA | Age (months):10.3 | WLZ:-2.7 | Helath phenotype:Malnourished	data table name=S5C; RAW_FILE_NAME=2028 08 03 Jimmy fecal sample-10
SUBJECT_SAMPLE_FACTORS           	-	PS.120-13.3	Cage:NA | Microbiota:NA | Diet:NA | Age (months):13.3 | WLZ:-3.16 | Helath phenotype:Malnourished	data table name=S5C; RAW_FILE_NAME=2029 08 03 Jimmy fecal sample-19
SUBJECT_SAMPLE_FACTORS           	-	PS.120-13.9	Cage:NA | Microbiota:NA | Diet:NA | Age (months):13.9 | WLZ:-2.17 | Helath phenotype:Malnourished	data table name=S5C; RAW_FILE_NAME=2030 08 03 Jimmy fecal sample-23
SUBJECT_SAMPLE_FACTORS           	-	PS.132	Cage:NA | Microbiota:NA | Diet:NA | Age (months):14.1 | WLZ:-3.01 | Helath phenotype:Malnourished	data table name=S5C; RAW_FILE_NAME=2031 08 03 Jimmy fecal sample-27
SUBJECT_SAMPLE_FACTORS           	-	PS.158	Cage:NA | Microbiota:NA | Diet:NA | Age (months):12.5 | WLZ:-3.48 | Helath phenotype:Malnourished	data table name=S5C; RAW_FILE_NAME=2029 08 03 Jimmy fecal sample-15
SUBJECT_SAMPLE_FACTORS           	-	PS.170	Cage:NA | Microbiota:NA | Diet:NA | Age (months):16.2 | WLZ:-3.5 | Helath phenotype:Malnourished	data table name=S5C; RAW_FILE_NAME=2030 08 03 Jimmy fecal sample-28
#COLLECTION
CO:COLLECTION_SUMMARY            	Cecal contents were collected on 10 uL plastic inoculation loops at the time of
CO:COLLECTION_SUMMARY            	sacrifice, snap frozen in liquid nitrogen, and stored at -80 °C. Aliquots were
CO:COLLECTION_SUMMARY            	kept frozen, removed from the loops and weighed.
CO:SAMPLE_TYPE                   	Cecum
CO:STORAGE_CONDITIONS            	-80℃
#TREATMENT
TR:TREATMENT_SUMMARY             	Germ-free C57BL/6J mice were reared in plastic flexible film gnotobiotic
TR:TREATMENT_SUMMARY             	isolators (Class Biologically Clean) at 23 °C under a strict 12-hour light
TR:TREATMENT_SUMMARY             	cycle (lights on at 0600h, off at 1800h). Animals were weaned onto an
TR:TREATMENT_SUMMARY             	autoclaved, low-fat, plant polysaccharide-rich chow (catalog number 2018S,
TR:TREATMENT_SUMMARY             	Envigo) that was administered ad libitum. Five days prior to colonization,
TR:TREATMENT_SUMMARY             	8-week-old male mice were switched to the NAD precursor-sufficient diet; 2 days
TR:TREATMENT_SUMMARY             	later, they were either placed on a NAD precursor-deficient diet, or a
TR:TREATMENT_SUMMARY             	NA-supplemented diet or were continued on the NAD precursor-sufficient diet.
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	10 uL of ice-cold methanol was added per mg of sample, which was then thawed on
SP:SAMPLEPREP_SUMMARY            	ice. The mixture was subsequently shaken for two minutes in a BioSpec bead
SP:SAMPLEPREP_SUMMARY            	beater. Samples were then centrifuged for 10 minutes at 3,000 x g at 4 °C; a
SP:SAMPLEPREP_SUMMARY            	150 uL aliquot of the supernatant was transferred to a new tube containing 150
SP:SAMPLEPREP_SUMMARY            	uL ice-cold chloroform, then mixed by pulse vortexing and centrifuged for 10
SP:SAMPLEPREP_SUMMARY            	minutes at 3,000 x g at 4 °C. 100 uL of the methanol fraction was transferred
SP:SAMPLEPREP_SUMMARY            	to a new tube containing 100 uL of ice-cold chloroform, mixed by
SP:SAMPLEPREP_SUMMARY            	pulse-vortexing, and centrifuged for 10 minutes at 3,000 x g at 4 °C. 70 uL was
SP:SAMPLEPREP_SUMMARY            	transferred to a new tube and lyophilized in a Labconco SpeedVac.
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_TYPE           	Reversed phase
CH:INSTRUMENT_NAME               	Agilent 1290 Infinity
CH:COLUMN_NAME                   	Waters Atlantis C18
#ANALYSIS
AN:ANALYSIS_TYPE                 	MS
#MS
MS:INSTRUMENT_NAME               	Agilent 6490 QQQ
MS:INSTRUMENT_TYPE               	Triple quadrupole
MS:MS_TYPE                       	ESI
MS:ION_MODE                      	POSITIVE
MS:MS_COMMENTS                   	Metabolites were quantified by MassHunter quantitative analysis tool (Agilent)
#MS_METABOLITE_DATA
MS_METABOLITE_DATA:UNITS	pmol/mg
MS_METABOLITE_DATA_START
Samples	m09a-S5A	m01b-S5A	m07b-S5A	m08a-S5A	m11b-S5A	m14a-S5A	m17a-S5A	m13b-S5A	m16a-S5A	m05b-S5A	m02a-S5A	m14b-S5A	m02b-S5A	m08b-S5A	m03b-S5A	m05a-S5A	m15a-S5A	m11a-S5A	m13a-S5A	m07a-S5A	m06b-S5A	m10b-S5A	m18a-S5A	m01a-S5A	m12b-S5A	m06a-S5A	m04b-S5A	m10a-S5A	m09b-S5A	m04a-S5A	m03a-S5A	m12a-S5A	m05b	m14a	m13a	m15a	m12a	m04c	m10a	m02c	m03b	m17a	m18a	m16a	m03c	m01c	m07a	m05c	m04d	m08c	m13b	m09b	m10b	m06b	m12b	m07b	m01a	m02a	m08a	m03a	m05a	m04a	m06a	m09a	m04b	m08b	m01b	m02b	m06c	m02d	m18b	m01d	m07c	m11b	m11a	m14b	m17b	m16b	m15b	m03d	BG1C0462	BG1C0466-12	BG1C0466-13	BG1C0473-11	BG1C0473-13	Bgsng8064	Bgsng8119	Bgsng8153	Bgsng8169-28	Bgsng8169-30	PS.004	PS.011	PS.057-10.4	PS.057-10.6	PS.079	PS.120-13.3	PS.120-13.9	PS.132	PS.158	PS.170
Factors	Cage:1 | Microbiota:Germfree | Diet:NAD-precursor sufficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	Cage:1 | Microbiota:Germfree | Diet:NAD-precursor sufficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	Cage:1 | Microbiota:Germfree | Diet:NAD-precursor sufficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	Cage:1 | Microbiota:Germfree | Diet:NAD-precursor sufficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	Cage:2 | Microbiota:Germfree | Diet:NAD-precursor sufficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	Cage:2 | Microbiota:Germfree | Diet:NAD-precursor sufficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	Cage:2 | Microbiota:Germfree | Diet:NAD-precursor sufficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	Cage:2 | Microbiota:Germfree | Diet:NAD-precursor sufficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	Cage:3 | Microbiota:Germfree | Diet:NAD-precursor deficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	Cage:3 | Microbiota:Germfree | Diet:NAD-precursor deficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	Cage:3 | Microbiota:Germfree | Diet:NAD-precursor deficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	Cage:3 | Microbiota:Germfree | Diet:NAD-precursor deficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	Cage:4 | Microbiota:Germfree | Diet:NAD-precursor deficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	Cage:4 | Microbiota:Germfree | Diet:NAD-precursor deficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	Cage:4 | Microbiota:Germfree | Diet:NAD-precursor deficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	Cage:4 | Microbiota:Germfree | Diet:NAD-precursor deficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	Cage:1 | Microbiota:26-member | Diet:NAD-precursor sufficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	Cage:1 | Microbiota:26-member | Diet:NAD-precursor sufficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	Cage:1 | Microbiota:26-member | Diet:NAD-precursor sufficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	Cage:1 | Microbiota:26-member | Diet:NAD-precursor sufficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	Cage:2 | Microbiota:26-member | Diet:NAD-precursor sufficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	Cage:2 | Microbiota:26-member | Diet:NAD-precursor sufficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	Cage:2 | Microbiota:26-member | Diet:NAD-precursor sufficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	Cage:2 | Microbiota:26-member | Diet:NAD-precursor sufficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	Cage:3 | Microbiota:26-member | Diet:NAD-precursor deficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	Cage:3 | Microbiota:26-member | Diet:NAD-precursor deficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	Cage:3 | Microbiota:26-member | Diet:NAD-precursor deficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	Cage:3 | Microbiota:26-member | Diet:NAD-precursor deficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	Cage:4 | Microbiota:26-member | Diet:NAD-precursor deficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	Cage:4 | Microbiota:26-member | Diet:NAD-precursor deficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	Cage:4 | Microbiota:26-member | Diet:NAD-precursor deficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	Cage:4 | Microbiota:26-member | Diet:NAD-precursor deficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	Cage:1 | Microbiota:Btheta | Diet:NAD-precursor deficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	Cage:1 | Microbiota:Btheta | Diet:NAD-precursor deficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	Cage:1 | Microbiota:Btheta | Diet:NAD-precursor deficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	Cage:2 | Microbiota:Btheta | Diet:NAD-precursor deficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	Cage:2 | Microbiota:Btheta | Diet:NAD-precursor deficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	Cage:2 | Microbiota:Btheta | Diet:NAD-precursor deficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	Cage:3 | Microbiota:Btheta | Diet:NA-supplementeded | Age (months):NA | WLZ:NA | Helath phenotype:NA	Cage:3 | Microbiota:Btheta | Diet:NA-supplementeded | Age (months):NA | WLZ:NA | Helath phenotype:NA	Cage:3 | Microbiota:Btheta | Diet:NA-supplementeded | Age (months):NA | WLZ:NA | Helath phenotype:NA	Cage:4 | Microbiota:Btheta | Diet:NA-supplementeded | Age (months):NA | WLZ:NA | Helath phenotype:NA	Cage:4 | Microbiota:Btheta | Diet:NA-supplementeded | Age (months):NA | WLZ:NA | Helath phenotype:NA	Cage:4 | Microbiota:Btheta | Diet:NA-supplementeded | Age (months):NA | WLZ:NA | Helath phenotype:NA	Cage:1 | Microbiota:Bxylan | Diet:NAD-precursor deficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	Cage:1 | Microbiota:Bxylan | Diet:NAD-precursor deficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	Cage:1 | Microbiota:Bxylan | Diet:NAD-precursor deficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	Cage:2 | Microbiota:Bxylan | Diet:NAD-precursor deficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	Cage:2 | Microbiota:Bxylan | Diet:NAD-precursor deficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	Cage:2 | Microbiota:Bxylan | Diet:NAD-precursor deficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	Cage:3 | Microbiota:Bxylan | Diet:NA-supplementeded | Age (months):NA | WLZ:NA | Helath phenotype:NA	Cage:3 | Microbiota:Bxylan | Diet:NA-supplementeded | Age (months):NA | WLZ:NA | Helath phenotype:NA	Cage:3 | Microbiota:Bxylan | Diet:NA-supplementeded | Age (months):NA | WLZ:NA | Helath phenotype:NA	Cage:4 | Microbiota:Bxylan | Diet:NA-supplementeded | Age (months):NA | WLZ:NA | Helath phenotype:NA	Cage:4 | Microbiota:Bxylan | Diet:NA-supplementeded | Age (months):NA | WLZ:NA | Helath phenotype:NA	Cage:4 | Microbiota:Bxylan | Diet:NA-supplementeded | Age (months):NA | WLZ:NA | Helath phenotype:NA	Cage:1 | Microbiota:Germfree | Diet:NAD-precursor deficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	Cage:1 | Microbiota:Germfree | Diet:NAD-precursor deficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	Cage:1 | Microbiota:Germfree | Diet:NAD-precursor deficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	Cage:2 | Microbiota:Germfree | Diet:NAD-precursor deficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	Cage:2 | Microbiota:Germfree | Diet:NAD-precursor deficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	Cage:2 | Microbiota:Germfree | Diet:NAD-precursor deficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	Cage:3 | Microbiota:Germfree | Diet:NA-supplementeded | Age (months):NA | WLZ:NA | Helath phenotype:NA	Cage:3 | Microbiota:Germfree | Diet:NA-supplementeded | Age (months):NA | WLZ:NA | Helath phenotype:NA	Cage:3 | Microbiota:Germfree | Diet:NA-supplementeded | Age (months):NA | WLZ:NA | Helath phenotype:NA	Cage:4 | Microbiota:Germfree | Diet:NA-supplementeded | Age (months):NA | WLZ:NA | Helath phenotype:NA	Cage:4 | Microbiota:Germfree | Diet:NA-supplementeded | Age (months):NA | WLZ:NA | Helath phenotype:NA	Cage:4 | Microbiota:Germfree | Diet:NA-supplementeded | Age (months):NA | WLZ:NA | Helath phenotype:NA	Cage:1 | Microbiota:26-member | Diet:NAD-precursor deficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	Cage:1 | Microbiota:26-member | Diet:NAD-precursor deficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	Cage:1 | Microbiota:26-member | Diet:NAD-precursor deficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	Cage:2 | Microbiota:26-member | Diet:NAD-precursor deficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	Cage:2 | Microbiota:26-member | Diet:NAD-precursor deficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	Cage:2 | Microbiota:26-member | Diet:NAD-precursor deficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	Cage:1 | Microbiota:BthetaBxylan | Diet:NAD-precursor deficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	Cage:1 | Microbiota:BthetaBxylan | Diet:NAD-precursor deficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	Cage:1 | Microbiota:BthetaBxylan | Diet:NAD-precursor deficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	Cage:2 | Microbiota:BthetaBxylan | Diet:NAD-precursor deficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	Cage:2 | Microbiota:BthetaBxylan | Diet:NAD-precursor deficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	Cage:2 | Microbiota:BthetaBxylan | Diet:NAD-precursor deficient | Age (months):NA | WLZ:NA | Helath phenotype:NA	Cage:NA | Microbiota:NA | Diet:NA | Age (months):8 | WLZ:-0.43 | Helath phenotype:Healthy	Cage:NA | Microbiota:NA | Diet:NA | Age (months):12 | WLZ:0.2 | Helath phenotype:Healthy	Cage:NA | Microbiota:NA | Diet:NA | Age (months):13 | WLZ:-0.3 | Helath phenotype:Healthy	Cage:NA | Microbiota:NA | Diet:NA | Age (months):11 | WLZ:-0.82 | Helath phenotype:Healthy	Cage:NA | Microbiota:NA | Diet:NA | Age (months):13 | WLZ:-0.93 | Helath phenotype:Healthy	Cage:NA | Microbiota:NA | Diet:NA | Age (months):15 | WLZ:0.6 | Helath phenotype:Healthy	Cage:NA | Microbiota:NA | Diet:NA | Age (months):25 | WLZ:1.7 | Helath phenotype:Healthy	Cage:NA | Microbiota:NA | Diet:NA | Age (months):6 | WLZ:1.58 | Helath phenotype:Healthy	Cage:NA | Microbiota:NA | Diet:NA | Age (months):28 | WLZ:- | Helath phenotype:Healthy	Cage:NA | Microbiota:NA | Diet:NA | Age (months):30 | WLZ:1.77 | Helath phenotype:Healthy	Cage:NA | Microbiota:NA | Diet:NA | Age (months):21.9 | WLZ:-2.03 | Helath phenotype:Malnourished	Cage:NA | Microbiota:NA | Diet:NA | Age (months):9.9 | WLZ:-5.54 | Helath phenotype:Malnourished	Cage:NA | Microbiota:NA | Diet:NA | Age (months):10.4 | WLZ:-3.8 | Helath phenotype:Malnourished	Cage:NA | Microbiota:NA | Diet:NA | Age (months):10.6 | WLZ:-2.81 | Helath phenotype:Malnourished	Cage:NA | Microbiota:NA | Diet:NA | Age (months):10.3 | WLZ:-2.7 | Helath phenotype:Malnourished	Cage:NA | Microbiota:NA | Diet:NA | Age (months):13.3 | WLZ:-3.16 | Helath phenotype:Malnourished	Cage:NA | Microbiota:NA | Diet:NA | Age (months):13.9 | WLZ:-2.17 | Helath phenotype:Malnourished	Cage:NA | Microbiota:NA | Diet:NA | Age (months):14.1 | WLZ:-3.01 | Helath phenotype:Malnourished	Cage:NA | Microbiota:NA | Diet:NA | Age (months):12.5 | WLZ:-3.48 | Helath phenotype:Malnourished	Cage:NA | Microbiota:NA | Diet:NA | Age (months):16.2 | WLZ:-3.5 | Helath phenotype:Malnourished
Aspartate	6.60	10.57	9.81	16.86	14.26	20.87	31.88	57.83	86.61	79.09	90.09	109.56	109.24	79.32	115.03	111.70	52.56	65.36	187.89	84.74	56.84	56.50	77.67	71.29	204.55	101.74	113.75	94.89	131.82	117.93	119.93	94.32	3.77	10.39	8.54	7.49	6.07	5.94	11.00	18.07	11.30	14.64	10.15	9.81	6.65	8.25	8.74	11.20	35.33	10.12	20.07	6.19	6.11	5.68	11.57	6.25	301.67	213.58	171.69	215.20	204.84	122.87	224.29	162.77	250.65	256.19	173.37	229.21	138.34	143.19	164.20	148.14	141.35	121.19	3.83	10.65	5.23	6.43	8.13	14.49	683.45	568.15	206.87	204.42	629.37	1879.09	79.05	134.22	138.89	346.13	59.15	23.87	181.00	192.52	6.29	15.14	70.17	439.48	92.88	111.64
NAD	0.027	0.012	0.022	0.023	0.009	0.027	0.021	0.008	0.019	0.018	0.015	0.028	0.015	0.011	0.042	0.024	0.037	0.015	0.067	0.098	0.023	0.030	0.041	0.021	0.018	0.020	0.015	0.017	0.020	0.024	0.017	0.027	2.927	0.525	1.507	0.320	0.315	0.983	1.049	1.365	2.451	1.082	2.109	1.630	0.751	0.199	0.110	0.416	0.546	0.239	1.264	0.580	1.341	1.049	1.306	0.457	0.416	0.228	0.250	0.197	0.216	0.161	0.373	0.200	0.173	0.311	0.223	0.265	1.182	0.749	0.970	0.631	0.513	0.674	0.612	0.535	1.496	0.539	0.783	0.499	0.011	0.039	0.138	0.062	0.855	0.078	0.085	0.050	0.089	0.036	0.154	0.230	0.513	1.075	0.390	0.250	0.329	0.459	0.582	0.712
Nam	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	0.067	0.103	0.187	0.111	0.116	0.067	4.986	4.234	5.367	3.043	3.602	2.786	0.075	0.077	0.078	0.181	0.582	0.096	2.701	2.216	1.657	2.211	1.486	1.552	0.019	0.005	0.066	0.013	0.009	0.011	1.254	1.137	1.106	2.639	1.638	1.457	0.195	0.141	0.144	0.182	0.111	0.073	0.103	0.073	0.090	0.065	0.159	0.109	0.000	0.000	0.002	0.002	0.000	0.000	0.011	0.150	0.000	0.000	0.000	0.000	0.000	0.000	0.000	0.000	0.000	0.008	0.000	0.000
Nicotinic acid	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	6.79	9.73	7.30	5.13	3.56	4.08	3.63	4.32	2.58	1.72	2.37	2.33	3.84	1.88	2.20	1.17	0.001	0.001	0.001	0.000	0.001	0.001	0.005	0.003	0.005	0.003	0.004	0.003	0.001	0.001	0.001	0.001	0.001	0.001	0.008	0.005	0.003	0.006	0.004	0.005	0.001	0.001	0.001	0.001	0.001	0.001	0.007	0.007	0.003	0.010	0.004	0.004	0.232	0.319	0.233	0.265	0.147	0.105	0.001	0.003	0.001	0.001	0.001	0.001	0.37	2.68	6.02	1.39	0.90	2.16	3.18	0.11	4.01	6.30	0.17	0.18	0.17	0.21	0.06	0.10	0.26	0.02	0.01	0.01
v-cADPR-x	0.005	0.005	0.005	0.004	0.006	0.003	0.002	0.006	0.005	0.005	0.003	0.007	0.003	0.003	0.005	0.003	0.010	0.007	0.016	0.010	0.011	0.010	0.010	0.010	0.015	0.010	0.010	0.007	0.008	0.011	0.007	0.010	0.180	0.150	0.160	0.157	0.174	0.147	0.134	0.149	0.156	0.169	0.170	0.209	0.838	0.767	0.619	0.935	0.867	0.879	0.479	0.672	1.213	1.148	0.815	0.757	0.181	0.190	0.177	0.220	0.209	0.132	0.153	0.153	0.157	0.206	0.115	0.205	0.338	0.431	0.278	0.284	0.261	0.261	0.200	0.304	0.347	0.356	0.277	0.194	0.003	0.005	0.011	0.010	0.009	0.061	0.007	0.003	0.002	0.003	0.003	0.003	0.002	0.005	0.002	0.002	0.006	0.005	0.005	0.002
MS_METABOLITE_DATA_END
#METABOLITES
METABOLITES_START
metabolite_name	PubChem	m/z
Aspartate	 5960  	134/74
NAD	5892  	664/428
Nam	936	123/80
Nicotinic acid	938  	124/78
v-cADPR-x	new in this study  	542/428
METABOLITES_END
#END