#METABOLOMICS WORKBENCH alexchao_20220422_055158 DATATRACK_ID:3214 STUDY_ID:ST002151 ANALYSIS_ID:AN003522 PROJECT_ID:PR001364
VERSION             	1
CREATED_ON             	April 22, 2022, 7:26 am
#PROJECT
PR:PROJECT_TITLE                 	Placenta NTA studies
PR:PROJECT_SUMMARY               	Multi-omics study on placental samples to characterize xenobiotics and
PR:PROJECT_SUMMARY               	epigenomic/transcriptomic changes associated with preeclampsia
PR:INSTITUTE                     	U.S. EPA
PR:LAST_NAME                     	Chao
PR:FIRST_NAME                    	Alex
PR:ADDRESS                       	109 TW Alexander Dr, Durham, NC 27709, USA
PR:EMAIL                         	chao.alex@epa.gov
PR:PHONE                         	9195414261
#STUDY
ST:STUDY_TITLE                   	Integrative Exposomic, Transcriptomic, Epigenomic Analyses of Human Placental
ST:STUDY_TITLE                   	Samples Links Understudied Chemicals to Preeclampsia
ST:STUDY_SUMMARY                 	Background Environmental health research has recently undergone a dramatic
ST:STUDY_SUMMARY                 	shift, with ongoing technological advancements allowing for broader coverage of
ST:STUDY_SUMMARY                 	exposure and molecular biology signatures. Approaches to integrate such measures
ST:STUDY_SUMMARY                 	are still needed to increase understanding between systems-level exposure and
ST:STUDY_SUMMARY                 	biology. Objectives We address this gap by evaluating placental tissues to
ST:STUDY_SUMMARY                 	identify novel chemical-biological interactions associated with preeclampsia.
ST:STUDY_SUMMARY                 	This study tests the hypothesis that understudied chemicals are present in the
ST:STUDY_SUMMARY                 	human placenta and associated with preeclampsia-relevant disruptions, including
ST:STUDY_SUMMARY                 	overall case status (preeclamptic vs. normotensive patients) and underlying
ST:STUDY_SUMMARY                 	transcriptomic/epigenomic signatures. Methods A non-targeted analysis based on
ST:STUDY_SUMMARY                 	high-resolution mass spectrometry was used to analyze placental tissues from a
ST:STUDY_SUMMARY                 	cohort of 35 patients with preeclampsia (n = 18) and normotensive (n = 17)
ST:STUDY_SUMMARY                 	pregnancies. Molecular feature data were queried against chemicals within the
ST:STUDY_SUMMARY                 	U.S. Environmental Protection Agency’s DSSTox database, and prioritized for
ST:STUDY_SUMMARY                 	confirmation based on association with preeclampsia case status and confidence
ST:STUDY_SUMMARY                 	of chemical identification. All molecular features were evaluated for
ST:STUDY_SUMMARY                 	relationships to mRNA, microRNA, and CpG methylation (i.e., multi-omic)
ST:STUDY_SUMMARY                 	signature alterations involved in preeclampsia. Results A total of 183 molecular
ST:STUDY_SUMMARY                 	features were identified with significantly differentiated abundance in
ST:STUDY_SUMMARY                 	placental extracts of preeclamptic patients; these features clustered into
ST:STUDY_SUMMARY                 	distinct chemical groupings using unsupervised methods. Of these features, 53
ST:STUDY_SUMMARY                 	were identified (mapping to 40 distinct chemicals) using chemical standards,
ST:STUDY_SUMMARY                 	fragmentation spectra, and chemical metadata. In general, human metabolites had
ST:STUDY_SUMMARY                 	the largest feature intensities and strongest associations with
ST:STUDY_SUMMARY                 	preeclampsia-relevant multi-omic changes. Exogenous drugs were second most
ST:STUDY_SUMMARY                 	abundant and had fewer associations with multi-omic changes. Other exogenous
ST:STUDY_SUMMARY                 	chemicals (non-drugs) were least abundant and had the fewest associations with
ST:STUDY_SUMMARY                 	multi-omic changes. Conclusions These global data trends suggest that human
ST:STUDY_SUMMARY                 	metabolites are heavily intertwined with biological processes involved in
ST:STUDY_SUMMARY                 	preeclampsia etiology, while exogenous chemicals may still impact select
ST:STUDY_SUMMARY                 	transcriptomic/epigenomic processes. This study serves as a demonstration of
ST:STUDY_SUMMARY                 	merging systems exposures with systems biology to better understand
ST:STUDY_SUMMARY                 	chemical-disease relationships.
ST:INSTITUTE                     	EPA
ST:LAST_NAME                     	Chao
ST:FIRST_NAME                    	Alex
ST:ADDRESS                       	109 TW Alexander Drive, Durham, NC 27709, USA
ST:EMAIL                         	chao.alex@epa.gov
ST:PHONE                         	9195414261
#SUBJECT
SU:SUBJECT_TYPE                  	Human
SU:SUBJECT_SPECIES               	Homo sapiens
SU:TAXONOMY_ID                   	9606
#FACTORS
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data
SUBJECT_SAMPLE_FACTORS           	-	Blank_Positive_1	Treatment:Blank	RAW_FILE_NAME=Blank_Positive_MS_EPA_1.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Blank_Positive_2	Treatment:Blank	RAW_FILE_NAME=Blank_Positive_MS_EPA_2.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Blank_Positive_3	Treatment:Blank	RAW_FILE_NAME=Blank_Positive_MS_EPA_3.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Method Blank_Positive_1	Treatment:Blank	RAW_FILE_NAME=Blank_Positive_MS_Method_1.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Method Blank_Positive_2	Treatment:Blank	RAW_FILE_NAME=Blank_Positive_MS_Method_2.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Method Blank_Positive_3	Treatment:Blank	RAW_FILE_NAME=Blank_Positive_MS_Method_3.mzdata
SUBJECT_SAMPLE_FACTORS           	-	P1_Positive_1	Treatment:Control	RAW_FILE_NAME=P1_Positive_MS_Placenta_1.mzdata
SUBJECT_SAMPLE_FACTORS           	-	P1_Positive_2	Treatment:Control	RAW_FILE_NAME=P1_Positive_MS_Placenta_2.mzdata
SUBJECT_SAMPLE_FACTORS           	-	P1_Positive_3	Treatment:Control	RAW_FILE_NAME=P1_Positive_MS_Placenta_3.mzdata
SUBJECT_SAMPLE_FACTORS           	-	P2_Positive_1	Treatment:Control	RAW_FILE_NAME=P2_Positive_MS_Placenta_1.mzdata
SUBJECT_SAMPLE_FACTORS           	-	P2_Positive_2	Treatment:Control	RAW_FILE_NAME=P2_Positive_MS_Placenta_2.mzdata
SUBJECT_SAMPLE_FACTORS           	-	P2_Positive_3	Treatment:Control	RAW_FILE_NAME=P2_Positive_MS_Placenta_3.mzdata
SUBJECT_SAMPLE_FACTORS           	-	P3_Positive_1	Treatment:Control	RAW_FILE_NAME=P3_Positive_MS_Placenta_1.mzdata
SUBJECT_SAMPLE_FACTORS           	-	P3_Positive_2	Treatment:Control	RAW_FILE_NAME=P3_Positive_MS_Placenta_2.mzdata
SUBJECT_SAMPLE_FACTORS           	-	P3_Positive_3	Treatment:Control	RAW_FILE_NAME=P3_Positive_MS_Placenta_3.mzdata
SUBJECT_SAMPLE_FACTORS           	-	P4_Positive_1	Treatment:Control	RAW_FILE_NAME=P4_Positive_MS_Placenta_1.mzdata
SUBJECT_SAMPLE_FACTORS           	-	P4_Positive_2	Treatment:Control	RAW_FILE_NAME=P4_Positive_MS_Placenta_2.mzdata
SUBJECT_SAMPLE_FACTORS           	-	P4_Positive_3	Treatment:Control	RAW_FILE_NAME=P4_Positive_MS_Placenta_3.mzdata
SUBJECT_SAMPLE_FACTORS           	-	P5_Positive_1	Treatment:Control	RAW_FILE_NAME=P5_Positive_MS_Placenta_1.mzdata
SUBJECT_SAMPLE_FACTORS           	-	P5_Positive_2	Treatment:Control	RAW_FILE_NAME=P5_Positive_MS_Placenta_2.mzdata
SUBJECT_SAMPLE_FACTORS           	-	P5_Positive_3	Treatment:Control	RAW_FILE_NAME=P5_Positive_MS_Placenta_3.mzdata
SUBJECT_SAMPLE_FACTORS           	-	P8_Positive_1	Treatment:Control	RAW_FILE_NAME=P8_Positive_MS_Placenta_1.mzdata
SUBJECT_SAMPLE_FACTORS           	-	P8_Positive_2	Treatment:Control	RAW_FILE_NAME=P8_Positive_MS_Placenta_2.mzdata
SUBJECT_SAMPLE_FACTORS           	-	P8_Positive_3	Treatment:Control	RAW_FILE_NAME=P8_Positive_MS_Placenta_3.mzdata
SUBJECT_SAMPLE_FACTORS           	-	P9_Positive_1	Treatment:Control	RAW_FILE_NAME=P9_Positive_MS_Placenta_1.mzdata
SUBJECT_SAMPLE_FACTORS           	-	P9_Positive_2	Treatment:Control	RAW_FILE_NAME=P9_Positive_MS_Placenta_2.mzdata
SUBJECT_SAMPLE_FACTORS           	-	P9_Positive_3	Treatment:Control	RAW_FILE_NAME=P9_Positive_MS_Placenta_3.mzdata
SUBJECT_SAMPLE_FACTORS           	-	P10_Positive_1	Treatment:Control	RAW_FILE_NAME=P10_Positive_MS_Placenta_1.mzdata
SUBJECT_SAMPLE_FACTORS           	-	P10_Positive_2	Treatment:Control	RAW_FILE_NAME=P10_Positive_MS_Placenta_2.mzdata
SUBJECT_SAMPLE_FACTORS           	-	P10_Positive_3	Treatment:Control	RAW_FILE_NAME=P10_Positive_MS_Placenta_3.mzdata
SUBJECT_SAMPLE_FACTORS           	-	P11_Positive_1	Treatment:Control	RAW_FILE_NAME=P11_Positive_MS_Placenta_1.mzdata
SUBJECT_SAMPLE_FACTORS           	-	P11_Positive_2	Treatment:Control	RAW_FILE_NAME=P11_Positive_MS_Placenta_2.mzdata
SUBJECT_SAMPLE_FACTORS           	-	P11_Positive_3	Treatment:Control	RAW_FILE_NAME=P11_Positive_MS_Placenta_3.mzdata
SUBJECT_SAMPLE_FACTORS           	-	P12_Positive_1	Treatment:Control	RAW_FILE_NAME=P12_Positive_MS_Placenta_1.mzdata
SUBJECT_SAMPLE_FACTORS           	-	P12_Positive_2	Treatment:Control	RAW_FILE_NAME=P12_Positive_MS_Placenta_2.mzdata
SUBJECT_SAMPLE_FACTORS           	-	P12_Positive_3	Treatment:Control	RAW_FILE_NAME=P12_Positive_MS_Placenta_3.mzdata
SUBJECT_SAMPLE_FACTORS           	-	P13_Positive_1	Treatment:Control	RAW_FILE_NAME=P13_Positive_MS_Placenta_1.mzdata
SUBJECT_SAMPLE_FACTORS           	-	P13_Positive_2	Treatment:Control	RAW_FILE_NAME=P13_Positive_MS_Placenta_2.mzdata
SUBJECT_SAMPLE_FACTORS           	-	P13_Positive_3	Treatment:Control	RAW_FILE_NAME=P13_Positive_MS_Placenta_3.mzdata
SUBJECT_SAMPLE_FACTORS           	-	P14_Positive_1	Treatment:Control	RAW_FILE_NAME=P14_Positive_MS_Placenta_1.mzdata
SUBJECT_SAMPLE_FACTORS           	-	P14_Positive_2	Treatment:Control	RAW_FILE_NAME=P14_Positive_MS_Placenta_2.mzdata
SUBJECT_SAMPLE_FACTORS           	-	P14_Positive_3	Treatment:Control	RAW_FILE_NAME=P14_Positive_MS_Placenta_3.mzdata
SUBJECT_SAMPLE_FACTORS           	-	P15_Positive_1	Treatment:Control	RAW_FILE_NAME=P15_Positive_MS_Placenta_1.mzdata
SUBJECT_SAMPLE_FACTORS           	-	P15_Positive_2	Treatment:Control	RAW_FILE_NAME=P15_Positive_MS_Placenta_2.mzdata
SUBJECT_SAMPLE_FACTORS           	-	P15_Positive_3	Treatment:Control	RAW_FILE_NAME=P15_Positive_MS_Placenta_3.mzdata
SUBJECT_SAMPLE_FACTORS           	-	P17_Positive_1	Treatment:Control	RAW_FILE_NAME=P17_Positive_MS_Placenta_1.mzdata
SUBJECT_SAMPLE_FACTORS           	-	P17_Positive_2	Treatment:Control	RAW_FILE_NAME=P17_Positive_MS_Placenta_2.mzdata
SUBJECT_SAMPLE_FACTORS           	-	P17_Positive_3	Treatment:Control	RAW_FILE_NAME=P17_Positive_MS_Placenta_3.mzdata
SUBJECT_SAMPLE_FACTORS           	-	P18_Positive_1	Treatment:Control	RAW_FILE_NAME=P18_Positive_MS_Placenta_1.mzdata
SUBJECT_SAMPLE_FACTORS           	-	P18_Positive_2	Treatment:Control	RAW_FILE_NAME=P18_Positive_MS_Placenta_2.mzdata
SUBJECT_SAMPLE_FACTORS           	-	P18_Positive_3	Treatment:Control	RAW_FILE_NAME=P18_Positive_MS_Placenta_3.mzdata
SUBJECT_SAMPLE_FACTORS           	-	P19_Positive_1	Treatment:Control	RAW_FILE_NAME=P19_Positive_MS_Placenta_1.mzdata
SUBJECT_SAMPLE_FACTORS           	-	P19_Positive_2	Treatment:Control	RAW_FILE_NAME=P19_Positive_MS_Placenta_2.mzdata
SUBJECT_SAMPLE_FACTORS           	-	P19_Positive_3	Treatment:Control	RAW_FILE_NAME=P19_Positive_MS_Placenta_3.mzdata
SUBJECT_SAMPLE_FACTORS           	-	P20_Positive_1	Treatment:Control	RAW_FILE_NAME=P20_Positive_MS_Placenta_1.mzdata
SUBJECT_SAMPLE_FACTORS           	-	P20_Positive_2	Treatment:Control	RAW_FILE_NAME=P20_Positive_MS_Placenta_2.mzdata
SUBJECT_SAMPLE_FACTORS           	-	P20_Positive_3	Treatment:Control	RAW_FILE_NAME=P20_Positive_MS_Placenta_3.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q1_Positive_1	Treatment:preeclampsia	RAW_FILE_NAME=Q1_Positive_MS_Placenta_1.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q1_Positive_2	Treatment:preeclampsia	RAW_FILE_NAME=Q1_Positive_MS_Placenta_2.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q1_Positive_3	Treatment:preeclampsia	RAW_FILE_NAME=Q1_Positive_MS_Placenta_3.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q3_Positive_1	Treatment:preeclampsia	RAW_FILE_NAME=Q3_Positive_MS_Placenta_1.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q3_Positive_2	Treatment:preeclampsia	RAW_FILE_NAME=Q3_Positive_MS_Placenta_2.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q3_Positive_3	Treatment:preeclampsia	RAW_FILE_NAME=Q3_Positive_MS_Placenta_3.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q4_Positive_1	Treatment:preeclampsia	RAW_FILE_NAME=Q4_Positive_MS_Placenta_1.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q4_Positive_2	Treatment:preeclampsia	RAW_FILE_NAME=Q4_Positive_MS_Placenta_2.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q4_Positive_3	Treatment:preeclampsia	RAW_FILE_NAME=Q4_Positive_MS_Placenta_3.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q5_Positive_1	Treatment:preeclampsia	RAW_FILE_NAME=Q5_Positive_MS_Placenta_1.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q5_Positive_2	Treatment:preeclampsia	RAW_FILE_NAME=Q5_Positive_MS_Placenta_2.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q5_Positive_3	Treatment:preeclampsia	RAW_FILE_NAME=Q5_Positive_MS_Placenta_3.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q6_Positive_1	Treatment:preeclampsia	RAW_FILE_NAME=Q6_Positive_MS_Placenta_1.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q6_Positive_2	Treatment:preeclampsia	RAW_FILE_NAME=Q6_Positive_MS_Placenta_2.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q6_Positive_3	Treatment:preeclampsia	RAW_FILE_NAME=Q6_Positive_MS_Placenta_3.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q7_Positive_1	Treatment:preeclampsia	RAW_FILE_NAME=Q7_Positive_MS_Placenta_1.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q7_Positive_2	Treatment:preeclampsia	RAW_FILE_NAME=Q7_Positive_MS_Placenta_2.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q7_Positive_3	Treatment:preeclampsia	RAW_FILE_NAME=Q7_Positive_MS_Placenta_3.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q8_Positive_1	Treatment:preeclampsia	RAW_FILE_NAME=Q8_Positive_MS_Placenta_1.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q8_Positive_2	Treatment:preeclampsia	RAW_FILE_NAME=Q8_Positive_MS_Placenta_2.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q8_Positive_3	Treatment:preeclampsia	RAW_FILE_NAME=Q8_Positive_MS_Placenta_3.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q9_Positive_1	Treatment:preeclampsia	RAW_FILE_NAME=Q9_Positive_MS_Placenta_1.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q9_Positive_2	Treatment:preeclampsia	RAW_FILE_NAME=Q9_Positive_MS_Placenta_2.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q9_Positive_3	Treatment:preeclampsia	RAW_FILE_NAME=Q9_Positive_MS_Placenta_3.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q10_Positive_1	Treatment:preeclampsia	RAW_FILE_NAME=Q10_Positive_MS_Placenta_1.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q10_Positive_2	Treatment:preeclampsia	RAW_FILE_NAME=Q10_Positive_MS_Placenta_2.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q10_Positive_3	Treatment:preeclampsia	RAW_FILE_NAME=Q10_Positive_MS_Placenta_3.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q11_Positive_1	Treatment:preeclampsia	RAW_FILE_NAME=Q11_Positive_MS_Placenta_1.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q11_Positive_2	Treatment:preeclampsia	RAW_FILE_NAME=Q11_Positive_MS_Placenta_2.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q11_Positive_3	Treatment:preeclampsia	RAW_FILE_NAME=Q11_Positive_MS_Placenta_3.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q12_Positive_1	Treatment:preeclampsia	RAW_FILE_NAME=Q12_Positive_MS_Placenta_1.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q12_Positive_2	Treatment:preeclampsia	RAW_FILE_NAME=Q12_Positive_MS_Placenta_2.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q12_Positive_3	Treatment:preeclampsia	RAW_FILE_NAME=Q12_Positive_MS_Placenta_3.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q13_Positive_PostspikeA_Positive_1	Treatment:preeclampsia	RAW_FILE_NAME=Q13_Positive_MS_Placenta_PostspikeA_1.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q13_Positive_PostspikeA_Positive_2	Treatment:preeclampsia	RAW_FILE_NAME=Q13_Positive_MS_Placenta_PostspikeA_2.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q13_Positive_PostspikeA_Positive_3	Treatment:preeclampsia	RAW_FILE_NAME=Q13_Positive_MS_Placenta_PostspikeA_3.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q13_Positive_PostspikeB_Positive_1	Treatment:preeclampsia	RAW_FILE_NAME=Q13_Positive_MS_Placenta_PostspikeB_1.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q13_Positive_PostspikeB_Positive_2	Treatment:preeclampsia	RAW_FILE_NAME=Q13_Positive_MS_Placenta_PostspikeB_2.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q13_Positive_PostspikeB_Positive_3	Treatment:preeclampsia	RAW_FILE_NAME=Q13_Positive_MS_Placenta_PostspikeB_3.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q13_Positive_PrespikeA_Positive_1	Treatment:preeclampsia	RAW_FILE_NAME=Q13_Positive_MS_Placenta_PrespikeA_1.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q13_Positive_PrespikeA_Positive_2	Treatment:preeclampsia	RAW_FILE_NAME=Q13_Positive_MS_Placenta_PrespikeA_2.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q13_Positive_PrespikeA_Positive_3	Treatment:preeclampsia	RAW_FILE_NAME=Q13_Positive_MS_Placenta_PrespikeA_3.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q13_Positive_PrespikeB_Positive_1	Treatment:preeclampsia	RAW_FILE_NAME=Q13_Positive_MS_Placenta_PrespikeB_1.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q13_Positive_PrespikeB_Positive_2	Treatment:preeclampsia	RAW_FILE_NAME=Q13_Positive_MS_Placenta_PrespikeB_2.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q13_Positive_PrespikeB_Positive_3	Treatment:preeclampsia	RAW_FILE_NAME=Q13_Positive_MS_Placenta_PrespikeB_3.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q14_Positive_1	Treatment:preeclampsia	RAW_FILE_NAME=Q14_Positive_MS_Placenta_1.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q14_Positive_2	Treatment:preeclampsia	RAW_FILE_NAME=Q14_Positive_MS_Placenta_2.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q14_Positive_3	Treatment:preeclampsia	RAW_FILE_NAME=Q14_Positive_MS_Placenta_3.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q15_Positive_1	Treatment:preeclampsia	RAW_FILE_NAME=Q15_Positive_MS_Placenta_1.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q15_Positive_2	Treatment:preeclampsia	RAW_FILE_NAME=Q15_Positive_MS_Placenta_2.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q15_Positive_3	Treatment:preeclampsia	RAW_FILE_NAME=Q15_Positive_MS_Placenta_3.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q17_Positive_1	Treatment:preeclampsia	RAW_FILE_NAME=Q17_Positive_MS_Placenta_1.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q17_Positive_2	Treatment:preeclampsia	RAW_FILE_NAME=Q17_Positive_MS_Placenta_2.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q17_Positive_3	Treatment:preeclampsia	RAW_FILE_NAME=Q17_Positive_MS_Placenta_3.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q18_Positive_1	Treatment:preeclampsia	RAW_FILE_NAME=Q18_Positive_MS_Placenta_1.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q18_Positive_2	Treatment:preeclampsia	RAW_FILE_NAME=Q18_Positive_MS_Placenta_2.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q18_Positive_3	Treatment:preeclampsia	RAW_FILE_NAME=Q18_Positive_MS_Placenta_3.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q19_Positive_1	Treatment:preeclampsia	RAW_FILE_NAME=Q19_Positive_MS_Placenta_1.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q19_Positive_2	Treatment:preeclampsia	RAW_FILE_NAME=Q19_Positive_MS_Placenta_2.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q19_Positive_3	Treatment:preeclampsia	RAW_FILE_NAME=Q19_Positive_MS_Placenta_3.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q20_Positive_1	Treatment:preeclampsia	RAW_FILE_NAME=Q20_Positive_MS_Placenta_1.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q20_Positive_2	Treatment:preeclampsia	RAW_FILE_NAME=Q20_Positive_MS_Placenta_2.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q20_Positive_3	Treatment:preeclampsia	RAW_FILE_NAME=Q20_Positive_MS_Placenta_3.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Blank_Negative_1	Treatment:Blank	RAW_FILE_NAME=Blank_Negative_MS_EPA_1.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Blank_Negative_2	Treatment:Blank	RAW_FILE_NAME=Blank_Negative_MS_EPA_2.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Blank_Negative_3	Treatment:Blank	RAW_FILE_NAME=Blank_Negative_MS_EPA_3.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Method Blank_Negative_1	Treatment:Blank	RAW_FILE_NAME=Blank_Negative_MS_Method_1.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Method Blank_Negative_2	Treatment:Blank	RAW_FILE_NAME=Blank_Negative_MS_Method_2.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Method Blank_Negative_3	Treatment:Blank	RAW_FILE_NAME=Blank_Negative_MS_Method_3.mzdata
SUBJECT_SAMPLE_FACTORS           	-	P1_Negative_1	Treatment:Control	RAW_FILE_NAME=P1_Negative_MS_Placenta_1.mzdata
SUBJECT_SAMPLE_FACTORS           	-	P1_Negative_2	Treatment:Control	RAW_FILE_NAME=P1_Negative_MS_Placenta_2.mzdata
SUBJECT_SAMPLE_FACTORS           	-	P1_Negative_3	Treatment:Control	RAW_FILE_NAME=P1_Negative_MS_Placenta_3.mzdata
SUBJECT_SAMPLE_FACTORS           	-	P2_Negative_1	Treatment:Control	RAW_FILE_NAME=P2_Negative_MS_Placenta_1.mzdata
SUBJECT_SAMPLE_FACTORS           	-	P2_Negative_2	Treatment:Control	RAW_FILE_NAME=P2_Negative_MS_Placenta_2.mzdata
SUBJECT_SAMPLE_FACTORS           	-	P2_Negative_3	Treatment:Control	RAW_FILE_NAME=P2_Negative_MS_Placenta_3.mzdata
SUBJECT_SAMPLE_FACTORS           	-	P3_Negative_1	Treatment:Control	RAW_FILE_NAME=P3_Negative_MS_Placenta_1.mzdata
SUBJECT_SAMPLE_FACTORS           	-	P3_Negative_2	Treatment:Control	RAW_FILE_NAME=P3_Negative_MS_Placenta_2.mzdata
SUBJECT_SAMPLE_FACTORS           	-	P3_Negative_3	Treatment:Control	RAW_FILE_NAME=P3_Negative_MS_Placenta_3.mzdata
SUBJECT_SAMPLE_FACTORS           	-	P4_Negative_1	Treatment:Control	RAW_FILE_NAME=P4_Negative_MS_Placenta_1.mzdata
SUBJECT_SAMPLE_FACTORS           	-	P4_Negative_2	Treatment:Control	RAW_FILE_NAME=P4_Negative_MS_Placenta_2.mzdata
SUBJECT_SAMPLE_FACTORS           	-	P4_Negative_3	Treatment:Control	RAW_FILE_NAME=P4_Negative_MS_Placenta_3.mzdata
SUBJECT_SAMPLE_FACTORS           	-	P5_Negative_1	Treatment:Control	RAW_FILE_NAME=P5_Negative_MS_Placenta_1.mzdata
SUBJECT_SAMPLE_FACTORS           	-	P5_Negative_2	Treatment:Control	RAW_FILE_NAME=P5_Negative_MS_Placenta_2.mzdata
SUBJECT_SAMPLE_FACTORS           	-	P5_Negative_3	Treatment:Control	RAW_FILE_NAME=P5_Negative_MS_Placenta_3.mzdata
SUBJECT_SAMPLE_FACTORS           	-	P8_Negative_1	Treatment:Control	RAW_FILE_NAME=P8_Negative_MS_Placenta_1.mzdata
SUBJECT_SAMPLE_FACTORS           	-	P8_Negative_2	Treatment:Control	RAW_FILE_NAME=P8_Negative_MS_Placenta_2.mzdata
SUBJECT_SAMPLE_FACTORS           	-	P8_Negative_3	Treatment:Control	RAW_FILE_NAME=P8_Negative_MS_Placenta_3.mzdata
SUBJECT_SAMPLE_FACTORS           	-	P9_Negative_1	Treatment:Control	RAW_FILE_NAME=P9_Negative_MS_Placenta_1.mzdata
SUBJECT_SAMPLE_FACTORS           	-	P9_Negative_2	Treatment:Control	RAW_FILE_NAME=P9_Negative_MS_Placenta_2.mzdata
SUBJECT_SAMPLE_FACTORS           	-	P9_Negative_3	Treatment:Control	RAW_FILE_NAME=P9_Negative_MS_Placenta_3.mzdata
SUBJECT_SAMPLE_FACTORS           	-	P10_Negative_1	Treatment:Control	RAW_FILE_NAME=P10_Negative_MS_Placenta_1.mzdata
SUBJECT_SAMPLE_FACTORS           	-	P10_Negative_2	Treatment:Control	RAW_FILE_NAME=P10_Negative_MS_Placenta_2.mzdata
SUBJECT_SAMPLE_FACTORS           	-	P10_Negative_3	Treatment:Control	RAW_FILE_NAME=P10_Negative_MS_Placenta_3.mzdata
SUBJECT_SAMPLE_FACTORS           	-	P11_Negative_1	Treatment:Control	RAW_FILE_NAME=P11_Negative_MS_Placenta_1.mzdata
SUBJECT_SAMPLE_FACTORS           	-	P11_Negative_2	Treatment:Control	RAW_FILE_NAME=P11_Negative_MS_Placenta_2.mzdata
SUBJECT_SAMPLE_FACTORS           	-	P11_Negative_3	Treatment:Control	RAW_FILE_NAME=P11_Negative_MS_Placenta_3.mzdata
SUBJECT_SAMPLE_FACTORS           	-	P12_Negative_1	Treatment:Control	RAW_FILE_NAME=P12_Negative_MS_Placenta_1.mzdata
SUBJECT_SAMPLE_FACTORS           	-	P12_Negative_2	Treatment:Control	RAW_FILE_NAME=P12_Negative_MS_Placenta_2.mzdata
SUBJECT_SAMPLE_FACTORS           	-	P12_Negative_3	Treatment:Control	RAW_FILE_NAME=P12_Negative_MS_Placenta_3.mzdata
SUBJECT_SAMPLE_FACTORS           	-	P13_Negative_1	Treatment:Control	RAW_FILE_NAME=P13_Negative_MS_Placenta_1.mzdata
SUBJECT_SAMPLE_FACTORS           	-	P13_Negative_2	Treatment:Control	RAW_FILE_NAME=P13_Negative_MS_Placenta_2.mzdata
SUBJECT_SAMPLE_FACTORS           	-	P13_Negative_3	Treatment:Control	RAW_FILE_NAME=P13_Negative_MS_Placenta_3.mzdata
SUBJECT_SAMPLE_FACTORS           	-	P14_Negative_1	Treatment:Control	RAW_FILE_NAME=P14_Negative_MS_Placenta_1.mzdata
SUBJECT_SAMPLE_FACTORS           	-	P14_Negative_2	Treatment:Control	RAW_FILE_NAME=P14_Negative_MS_Placenta_2.mzdata
SUBJECT_SAMPLE_FACTORS           	-	P14_Negative_3	Treatment:Control	RAW_FILE_NAME=P14_Negative_MS_Placenta_3.mzdata
SUBJECT_SAMPLE_FACTORS           	-	P15_Negative_1	Treatment:Control	RAW_FILE_NAME=P15_Negative_MS_Placenta_1.mzdata
SUBJECT_SAMPLE_FACTORS           	-	P15_Negative_2	Treatment:Control	RAW_FILE_NAME=P15_Negative_MS_Placenta_2.mzdata
SUBJECT_SAMPLE_FACTORS           	-	P15_Negative_3	Treatment:Control	RAW_FILE_NAME=P15_Negative_MS_Placenta_3.mzdata
SUBJECT_SAMPLE_FACTORS           	-	P17_Negative_1	Treatment:Control	RAW_FILE_NAME=P17_Negative_MS_Placenta_1.mzdata
SUBJECT_SAMPLE_FACTORS           	-	P17_Negative_2	Treatment:Control	RAW_FILE_NAME=P17_Negative_MS_Placenta_2.mzdata
SUBJECT_SAMPLE_FACTORS           	-	P17_Negative_3	Treatment:Control	RAW_FILE_NAME=P17_Negative_MS_Placenta_3.mzdata
SUBJECT_SAMPLE_FACTORS           	-	P18_Negative_1	Treatment:Control	RAW_FILE_NAME=P18_Negative_MS_Placenta_1.mzdata
SUBJECT_SAMPLE_FACTORS           	-	P18_Negative_2	Treatment:Control	RAW_FILE_NAME=P18_Negative_MS_Placenta_2.mzdata
SUBJECT_SAMPLE_FACTORS           	-	P18_Negative_3	Treatment:Control	RAW_FILE_NAME=P18_Negative_MS_Placenta_3.mzdata
SUBJECT_SAMPLE_FACTORS           	-	P19_Negative_1	Treatment:Control	RAW_FILE_NAME=P19_Negative_MS_Placenta_1.mzdata
SUBJECT_SAMPLE_FACTORS           	-	P19_Negative_2	Treatment:Control	RAW_FILE_NAME=P19_Negative_MS_Placenta_2.mzdata
SUBJECT_SAMPLE_FACTORS           	-	P19_Negative_3	Treatment:Control	RAW_FILE_NAME=P19_Negative_MS_Placenta_3.mzdata
SUBJECT_SAMPLE_FACTORS           	-	P20_Negative_1	Treatment:Control	RAW_FILE_NAME=P20_Negative_MS_Placenta_1.mzdata
SUBJECT_SAMPLE_FACTORS           	-	P20_Negative_2	Treatment:Control	RAW_FILE_NAME=P20_Negative_MS_Placenta_2.mzdata
SUBJECT_SAMPLE_FACTORS           	-	P20_Negative_3	Treatment:Control	RAW_FILE_NAME=P20_Negative_MS_Placenta_3.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q1_Negative_1	Treatment:preeclampsia	RAW_FILE_NAME=Q1_Negative_MS_Placenta_1.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q1_Negative_2	Treatment:preeclampsia	RAW_FILE_NAME=Q1_Negative_MS_Placenta_2.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q1_Negative_3	Treatment:preeclampsia	RAW_FILE_NAME=Q1_Negative_MS_Placenta_3.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q3_Negative_1	Treatment:preeclampsia	RAW_FILE_NAME=Q3_Negative_MS_Placenta_1.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q3_Negative_2	Treatment:preeclampsia	RAW_FILE_NAME=Q3_Negative_MS_Placenta_2.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q3_Negative_3	Treatment:preeclampsia	RAW_FILE_NAME=Q3_Negative_MS_Placenta_3.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q4_Negative_1	Treatment:preeclampsia	RAW_FILE_NAME=Q4_Negative_MS_Placenta_1.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q4_Negative_2	Treatment:preeclampsia	RAW_FILE_NAME=Q4_Negative_MS_Placenta_2.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q4_Negative_3	Treatment:preeclampsia	RAW_FILE_NAME=Q4_Negative_MS_Placenta_3.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q5_Negative_1	Treatment:preeclampsia	RAW_FILE_NAME=Q5_Negative_MS_Placenta_1.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q5_Negative_2	Treatment:preeclampsia	RAW_FILE_NAME=Q5_Negative_MS_Placenta_2.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q5_Negative_3	Treatment:preeclampsia	RAW_FILE_NAME=Q5_Negative_MS_Placenta_3.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q6_Negative_1	Treatment:preeclampsia	RAW_FILE_NAME=Q6_Negative_MS_Placenta_1.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q6_Negative_2	Treatment:preeclampsia	RAW_FILE_NAME=Q6_Negative_MS_Placenta_2.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q6_Negative_3	Treatment:preeclampsia	RAW_FILE_NAME=Q6_Negative_MS_Placenta_3.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q7_Negative_1	Treatment:preeclampsia	RAW_FILE_NAME=Q7_Negative_MS_Placenta_1.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q7_Negative_2	Treatment:preeclampsia	RAW_FILE_NAME=Q7_Negative_MS_Placenta_2.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q7_Negative_3	Treatment:preeclampsia	RAW_FILE_NAME=Q7_Negative_MS_Placenta_3.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q8_Negative_1	Treatment:preeclampsia	RAW_FILE_NAME=Q8_Negative_MS_Placenta_1.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q8_Negative_2	Treatment:preeclampsia	RAW_FILE_NAME=Q8_Negative_MS_Placenta_2.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q8_Negative_3	Treatment:preeclampsia	RAW_FILE_NAME=Q8_Negative_MS_Placenta_3.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q9_Negative_1	Treatment:preeclampsia	RAW_FILE_NAME=Q9_Negative_MS_Placenta_1.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q9_Negative_2	Treatment:preeclampsia	RAW_FILE_NAME=Q9_Negative_MS_Placenta_2.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q9_Negative_3	Treatment:preeclampsia	RAW_FILE_NAME=Q9_Negative_MS_Placenta_3.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q10_Negative_1	Treatment:preeclampsia	RAW_FILE_NAME=Q10_Negative_MS_Placenta_1.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q10_Negative_2	Treatment:preeclampsia	RAW_FILE_NAME=Q10_Negative_MS_Placenta_2.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q10_Negative_3	Treatment:preeclampsia	RAW_FILE_NAME=Q10_Negative_MS_Placenta_3.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q11_Negative_1	Treatment:preeclampsia	RAW_FILE_NAME=Q11_Negative_MS_Placenta_1.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q11_Negative_2	Treatment:preeclampsia	RAW_FILE_NAME=Q11_Negative_MS_Placenta_2.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q11_Negative_3	Treatment:preeclampsia	RAW_FILE_NAME=Q11_Negative_MS_Placenta_3.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q12_Negative_1	Treatment:preeclampsia	RAW_FILE_NAME=Q12_Negative_MS_Placenta_1.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q12_Negative_2	Treatment:preeclampsia	RAW_FILE_NAME=Q12_Negative_MS_Placenta_2.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q12_Negative_3	Treatment:preeclampsia	RAW_FILE_NAME=Q12_Negative_MS_Placenta_3.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q13_Negative_PostspikeA_Negative_1	Treatment:preeclampsia	RAW_FILE_NAME=Q13_Negative_MS_Placenta_PostspikeA_1.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q13_Negative_PostspikeA_Negative_2	Treatment:preeclampsia	RAW_FILE_NAME=Q13_Negative_MS_Placenta_PostspikeA_2.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q13_Negative_PostspikeA_Negative_3	Treatment:preeclampsia	RAW_FILE_NAME=Q13_Negative_MS_Placenta_PostspikeA_3.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q13_Negative_PostspikeB_Negative_1	Treatment:preeclampsia	RAW_FILE_NAME=Q13_Negative_MS_Placenta_PostspikeB_1.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q13_Negative_PostspikeB_Negative_2	Treatment:preeclampsia	RAW_FILE_NAME=Q13_Negative_MS_Placenta_PostspikeB_2.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q13_Negative_PostspikeB_Negative_3	Treatment:preeclampsia	RAW_FILE_NAME=Q13_Negative_MS_Placenta_PostspikeB_3.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q13_Negative_PrespikeA_Negative_1	Treatment:preeclampsia	RAW_FILE_NAME=Q13_Negative_MS_Placenta_PrespikeA_1.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q13_Negative_PrespikeA_Negative_2	Treatment:preeclampsia	RAW_FILE_NAME=Q13_Negative_MS_Placenta_PrespikeA_2.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q13_Negative_PrespikeA_Negative_3	Treatment:preeclampsia	RAW_FILE_NAME=Q13_Negative_MS_Placenta_PrespikeA_3.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q13_Negative_PrespikeB_Negative_1	Treatment:preeclampsia	RAW_FILE_NAME=Q13_Negative_MS_Placenta_PrespikeB_1.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q13_Negative_PrespikeB_Negative_2	Treatment:preeclampsia	RAW_FILE_NAME=Q13_Negative_MS_Placenta_PrespikeB_2.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q13_Negative_PrespikeB_Negative_3	Treatment:preeclampsia	RAW_FILE_NAME=Q13_Negative_MS_Placenta_PrespikeB_3.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q14_Negative_1	Treatment:preeclampsia	RAW_FILE_NAME=Q14_Negative_MS_Placenta_1.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q14_Negative_2	Treatment:preeclampsia	RAW_FILE_NAME=Q14_Negative_MS_Placenta_2.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q14_Negative_3	Treatment:preeclampsia	RAW_FILE_NAME=Q14_Negative_MS_Placenta_3.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q15_Negative_1	Treatment:preeclampsia	RAW_FILE_NAME=Q15_Negative_MS_Placenta_1.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q15_Negative_2	Treatment:preeclampsia	RAW_FILE_NAME=Q15_Negative_MS_Placenta_2.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q15_Negative_3	Treatment:preeclampsia	RAW_FILE_NAME=Q15_Negative_MS_Placenta_3.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q17_Negative_1	Treatment:preeclampsia	RAW_FILE_NAME=Q17_Negative_MS_Placenta_1.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q17_Negative_2	Treatment:preeclampsia	RAW_FILE_NAME=Q17_Negative_MS_Placenta_2.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q17_Negative_3	Treatment:preeclampsia	RAW_FILE_NAME=Q17_Negative_MS_Placenta_3.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q18_Negative_1	Treatment:preeclampsia	RAW_FILE_NAME=Q18_Negative_MS_Placenta_1.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q18_Negative_2	Treatment:preeclampsia	RAW_FILE_NAME=Q18_Negative_MS_Placenta_2.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q18_Negative_3	Treatment:preeclampsia	RAW_FILE_NAME=Q18_Negative_MS_Placenta_3.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q19_Negative_1	Treatment:preeclampsia	RAW_FILE_NAME=Q19_Negative_MS_Placenta_1.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q19_Negative_2	Treatment:preeclampsia	RAW_FILE_NAME=Q19_Negative_MS_Placenta_2.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q19_Negative_3	Treatment:preeclampsia	RAW_FILE_NAME=Q19_Negative_MS_Placenta_3.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q20_Negative_1	Treatment:preeclampsia	RAW_FILE_NAME=Q20_Negative_MS_Placenta_1.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q20_Negative_2	Treatment:preeclampsia	RAW_FILE_NAME=Q20_Negative_MS_Placenta_2.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Q20_Negative_3	Treatment:preeclampsia	RAW_FILE_NAME=Q20_Negative_MS_Placenta_3.mzdata
SUBJECT_SAMPLE_FACTORS           	-	P1_Positive_MSMS	Treatment:Control	RAW_FILE_NAME=P1_Positive_MS_Placenta_MSMS.mgf
SUBJECT_SAMPLE_FACTORS           	-	P2_Positive_MSMS	Treatment:Control	RAW_FILE_NAME=P2_Positive_MS_Placenta_MSMS.mgf
SUBJECT_SAMPLE_FACTORS           	-	P3_Positive_MSMS	Treatment:Control	RAW_FILE_NAME=P3_Positive_MS_Placenta_MSMS.mgf
SUBJECT_SAMPLE_FACTORS           	-	P4_Positive_MSMS	Treatment:Control	RAW_FILE_NAME=P4_Positive_MS_Placenta_MSMS.mgf
SUBJECT_SAMPLE_FACTORS           	-	P5_Positive_MSMS	Treatment:Control	RAW_FILE_NAME=P5_Positive_MS_Placenta_MSMS.mgf
SUBJECT_SAMPLE_FACTORS           	-	P8_Positive_MSMS	Treatment:Control	RAW_FILE_NAME=P8_Positive_MS_Placenta_MSMS.mgf
SUBJECT_SAMPLE_FACTORS           	-	P9_Positive_MSMS	Treatment:Control	RAW_FILE_NAME=P9_Positive_MS_Placenta_MSMS.mgf
SUBJECT_SAMPLE_FACTORS           	-	P10_Positive_MSMS	Treatment:Control	RAW_FILE_NAME=P10_Positive_MS_Placenta_MSMS.mgf
SUBJECT_SAMPLE_FACTORS           	-	P11_Positive_MSMS	Treatment:Control	RAW_FILE_NAME=P11_Positive_MS_Placenta_MSMS.mgf
SUBJECT_SAMPLE_FACTORS           	-	P12_Positive_MSMS	Treatment:Control	RAW_FILE_NAME=P12_Positive_MS_Placenta_MSMS.mgf
SUBJECT_SAMPLE_FACTORS           	-	P13_Positive_MSMS	Treatment:Control	RAW_FILE_NAME=P13_Positive_MS_Placenta_MSMS.mgf
SUBJECT_SAMPLE_FACTORS           	-	P14_Positive_MSMS	Treatment:Control	RAW_FILE_NAME=P14_Positive_MS_Placenta_MSMS.mgf
SUBJECT_SAMPLE_FACTORS           	-	P15_Positive_MSMS	Treatment:Control	RAW_FILE_NAME=P15_Positive_MS_Placenta_MSMS.mgf
SUBJECT_SAMPLE_FACTORS           	-	P17_Positive_MSMS	Treatment:Control	RAW_FILE_NAME=P17_Positive_MS_Placenta_MSMS.mgf
SUBJECT_SAMPLE_FACTORS           	-	P18_Positive_MSMS	Treatment:Control	RAW_FILE_NAME=P18_Positive_MS_Placenta_MSMS.mgf
SUBJECT_SAMPLE_FACTORS           	-	P19_Positive_MSMS	Treatment:Control	RAW_FILE_NAME=P19_Positive_MS_Placenta_MSMS.mgf
SUBJECT_SAMPLE_FACTORS           	-	P20_Positive_MSMS	Treatment:Control	RAW_FILE_NAME=P20_Positive_MS_Placenta_MSMS.mgf
SUBJECT_SAMPLE_FACTORS           	-	Q1_Positive_MSMS	Treatment:preeclampsia	RAW_FILE_NAME=Q1_Positive_MS_Placenta_MSMS.mgf
SUBJECT_SAMPLE_FACTORS           	-	Q3_Positive_MSMS	Treatment:preeclampsia	RAW_FILE_NAME=Q3_Positive_MS_Placenta_MSMS.mgf
SUBJECT_SAMPLE_FACTORS           	-	Q4_Positive_MSMS	Treatment:preeclampsia	RAW_FILE_NAME=Q4_Positive_MS_Placenta_MSMS.mgf
SUBJECT_SAMPLE_FACTORS           	-	Q5_Positive_MSMS	Treatment:preeclampsia	RAW_FILE_NAME=Q5_Positive_MS_Placenta_MSMS.mgf
SUBJECT_SAMPLE_FACTORS           	-	Q6_Positive_MSMS	Treatment:preeclampsia	RAW_FILE_NAME=Q6_Positive_MS_Placenta_MSMS.mgf
SUBJECT_SAMPLE_FACTORS           	-	Q7_Positive_MSMS	Treatment:preeclampsia	RAW_FILE_NAME=Q7_Positive_MS_Placenta_MSMS.mgf
SUBJECT_SAMPLE_FACTORS           	-	Q8_Positive_MSMS	Treatment:preeclampsia	RAW_FILE_NAME=Q8_Positive_MS_Placenta_MSMS.mgf
SUBJECT_SAMPLE_FACTORS           	-	Q9_Positive_MSMS	Treatment:preeclampsia	RAW_FILE_NAME=Q9_Positive_MS_Placenta_MSMS.mgf
SUBJECT_SAMPLE_FACTORS           	-	Q10_Positive_MSMS	Treatment:preeclampsia	RAW_FILE_NAME=Q10_Positive_MS_Placenta_MSMS.mgf
SUBJECT_SAMPLE_FACTORS           	-	Q11_Positive_MSMS	Treatment:preeclampsia	RAW_FILE_NAME=Q11_Positive_MS_Placenta_MSMS.mgf
SUBJECT_SAMPLE_FACTORS           	-	Q12_Positive_MSMS	Treatment:preeclampsia	RAW_FILE_NAME=Q12_Positive_MS_Placenta_MSMS.mgf
SUBJECT_SAMPLE_FACTORS           	-	Q13_Positive_PostspikeA_MSMS	Treatment:preeclampsia	RAW_FILE_NAME=Q13_Positive_MS_Placenta_PostspikeA_MSMS.mgf
SUBJECT_SAMPLE_FACTORS           	-	Q13_Positive_PostspikeB_MSMS	Treatment:preeclampsia	RAW_FILE_NAME=Q13_Positive_MS_Placenta_PostspikeB_MSMS.mgf
SUBJECT_SAMPLE_FACTORS           	-	Q13_Positive_PrespikeA_MSMS	Treatment:preeclampsia	RAW_FILE_NAME=Q13_Positive_MS_Placenta_PrespikeA_MSMS.mgf
SUBJECT_SAMPLE_FACTORS           	-	Q13_Positive_PrespikeB_MSMS	Treatment:preeclampsia	RAW_FILE_NAME=Q13_Positive_MS_Placenta_PrespikeB_MSMS.mgf
SUBJECT_SAMPLE_FACTORS           	-	Q14_Positive_MSMS	Treatment:preeclampsia	RAW_FILE_NAME=Q14_Positive_MS_Placenta_MSMS.mgf
SUBJECT_SAMPLE_FACTORS           	-	Q15_Positive_MSMS	Treatment:preeclampsia	RAW_FILE_NAME=Q15_Positive_MS_Placenta_MSMS.mgf
SUBJECT_SAMPLE_FACTORS           	-	Q17_Positive_MSMS	Treatment:preeclampsia	RAW_FILE_NAME=Q17_Positive_MS_Placenta_MSMS.mgf
SUBJECT_SAMPLE_FACTORS           	-	Q18_Positive_MSMS	Treatment:preeclampsia	RAW_FILE_NAME=Q18_Positive_MS_Placenta_MSMS.mgf
SUBJECT_SAMPLE_FACTORS           	-	Q19_Positive_MSMS	Treatment:preeclampsia	RAW_FILE_NAME=Q19_Positive_MS_Placenta_MSMS.mgf
SUBJECT_SAMPLE_FACTORS           	-	Q20_Positive_MSMS	Treatment:preeclampsia	RAW_FILE_NAME=Q20_Positive_MS_Placenta_MSMS.mgf
SUBJECT_SAMPLE_FACTORS           	-	P1_Negative_MSMS	Treatment:Control	RAW_FILE_NAME=P1_Negative_MS_Placenta_MSMS.mgf
SUBJECT_SAMPLE_FACTORS           	-	P2_Negative_MSMS	Treatment:Control	RAW_FILE_NAME=P2_Negative_MS_Placenta_MSMS.mgf
SUBJECT_SAMPLE_FACTORS           	-	P3_Negative_MSMS	Treatment:Control	RAW_FILE_NAME=P3_Negative_MS_Placenta_MSMS.mgf
SUBJECT_SAMPLE_FACTORS           	-	P4_Negative_MSMS	Treatment:Control	RAW_FILE_NAME=P4_Negative_MS_Placenta_MSMS.mgf
SUBJECT_SAMPLE_FACTORS           	-	P5_Negative_MSMS	Treatment:Control	RAW_FILE_NAME=P5_Negative_MS_Placenta_MSMS.mgf
SUBJECT_SAMPLE_FACTORS           	-	P8_Negative_MSMS	Treatment:Control	RAW_FILE_NAME=P8_Negative_MS_Placenta_MSMS.mgf
SUBJECT_SAMPLE_FACTORS           	-	P9_Negative_MSMS	Treatment:Control	RAW_FILE_NAME=P9_Negative_MS_Placenta_MSMS.mgf
SUBJECT_SAMPLE_FACTORS           	-	P10_Negative_MSMS	Treatment:Control	RAW_FILE_NAME=P10_Negative_MS_Placenta_MSMS.mgf
SUBJECT_SAMPLE_FACTORS           	-	P11_Negative_MSMS	Treatment:Control	RAW_FILE_NAME=P11_Negative_MS_Placenta_MSMS.mgf
SUBJECT_SAMPLE_FACTORS           	-	P12_Negative_MSMS	Treatment:Control	RAW_FILE_NAME=P12_Negative_MS_Placenta_MSMS.mgf
SUBJECT_SAMPLE_FACTORS           	-	P13_Negative_MSMS	Treatment:Control	RAW_FILE_NAME=P13_Negative_MS_Placenta_MSMS.mgf
SUBJECT_SAMPLE_FACTORS           	-	P14_Negative_MSMS	Treatment:Control	RAW_FILE_NAME=P14_Negative_MS_Placenta_MSMS.mgf
SUBJECT_SAMPLE_FACTORS           	-	P15_Negative_MSMS	Treatment:Control	RAW_FILE_NAME=P15_Negative_MS_Placenta_MSMS.mgf
SUBJECT_SAMPLE_FACTORS           	-	P17_Negative_MSMS	Treatment:Control	RAW_FILE_NAME=P17_Negative_MS_Placenta_MSMS.mgf
SUBJECT_SAMPLE_FACTORS           	-	P18_Negative_MSMS	Treatment:Control	RAW_FILE_NAME=P18_Negative_MS_Placenta_MSMS.mgf
SUBJECT_SAMPLE_FACTORS           	-	P19_Negative_MSMS	Treatment:Control	RAW_FILE_NAME=P19_Negative_MS_Placenta_MSMS.mgf
SUBJECT_SAMPLE_FACTORS           	-	P20_Negative_MSMS	Treatment:Control	RAW_FILE_NAME=P20_Negative_MS_Placenta_MSMS.mgf
SUBJECT_SAMPLE_FACTORS           	-	Q1_Negative_MSMS	Treatment:preeclampsia	RAW_FILE_NAME=Q1_Negative_MS_Placenta_MSMS.mgf
SUBJECT_SAMPLE_FACTORS           	-	Q3_Negative_MSMS	Treatment:preeclampsia	RAW_FILE_NAME=Q3_Negative_MS_Placenta_MSMS.mgf
SUBJECT_SAMPLE_FACTORS           	-	Q4_Negative_MSMS	Treatment:preeclampsia	RAW_FILE_NAME=Q4_Negative_MS_Placenta_MSMS.mgf
SUBJECT_SAMPLE_FACTORS           	-	Q5_Negative_MSMS	Treatment:preeclampsia	RAW_FILE_NAME=Q5_Negative_MS_Placenta_MSMS.mgf
SUBJECT_SAMPLE_FACTORS           	-	Q6_Negative_MSMS	Treatment:preeclampsia	RAW_FILE_NAME=Q6_Negative_MS_Placenta_MSMS.mgf
SUBJECT_SAMPLE_FACTORS           	-	Q7_Negative_MSMS	Treatment:preeclampsia	RAW_FILE_NAME=Q7_Negative_MS_Placenta_MSMS.mgf
SUBJECT_SAMPLE_FACTORS           	-	Q8_Negative_MSMS	Treatment:preeclampsia	RAW_FILE_NAME=Q8_Negative_MS_Placenta_MSMS.mgf
SUBJECT_SAMPLE_FACTORS           	-	Q9_Negative_MSMS	Treatment:preeclampsia	RAW_FILE_NAME=Q9_Negative_MS_Placenta_MSMS.mgf
SUBJECT_SAMPLE_FACTORS           	-	Q10_Negative_MSMS	Treatment:preeclampsia	RAW_FILE_NAME=Q10_Negative_MS_Placenta_MSMS.mgf
SUBJECT_SAMPLE_FACTORS           	-	Q11_Negative_MSMS	Treatment:preeclampsia	RAW_FILE_NAME=Q11_Negative_MS_Placenta_MSMS.mgf
SUBJECT_SAMPLE_FACTORS           	-	Q12_Negative_MSMS	Treatment:preeclampsia	RAW_FILE_NAME=Q12_Negative_MS_Placenta_MSMS.mgf
SUBJECT_SAMPLE_FACTORS           	-	Q13_Negative_PostspikeA_MSMS	Treatment:preeclampsia	RAW_FILE_NAME=Q13_Negative_MS_Placenta_PostspikeA_MSMS.mgf
SUBJECT_SAMPLE_FACTORS           	-	Q13_Negative_PostspikeB_MSMS	Treatment:preeclampsia	RAW_FILE_NAME=Q13_Negative_MS_Placenta_PostspikeB_MSMS.mgf
SUBJECT_SAMPLE_FACTORS           	-	Q13_Negative_PrespikeA_MSMS	Treatment:preeclampsia	RAW_FILE_NAME=Q13_Negative_MS_Placenta_PrespikeA_MSMS.mgf
SUBJECT_SAMPLE_FACTORS           	-	Q13_Negative_PrespikeB_MSMS	Treatment:preeclampsia	RAW_FILE_NAME=Q13_Negative_MS_Placenta_PrespikeB_MSMS.mgf
SUBJECT_SAMPLE_FACTORS           	-	Q14_Negative_MSMS	Treatment:preeclampsia	RAW_FILE_NAME=Q14_Negative_MS_Placenta_MSMS.mgf
SUBJECT_SAMPLE_FACTORS           	-	Q15_Negative_MSMS	Treatment:preeclampsia	RAW_FILE_NAME=Q15_Negative_MS_Placenta_MSMS.mgf
SUBJECT_SAMPLE_FACTORS           	-	Q17_Negative_MSMS	Treatment:preeclampsia	RAW_FILE_NAME=Q17_Negative_MS_Placenta_MSMS.mgf
SUBJECT_SAMPLE_FACTORS           	-	Q18_Negative_MSMS	Treatment:preeclampsia	RAW_FILE_NAME=Q18_Negative_MS_Placenta_MSMS.mgf
SUBJECT_SAMPLE_FACTORS           	-	Q19_Negative_MSMS	Treatment:preeclampsia	RAW_FILE_NAME=Q19_Negative_MS_Placenta_MSMS.mgf
SUBJECT_SAMPLE_FACTORS           	-	Q20_Negative_MSMS	Treatment:preeclampsia	RAW_FILE_NAME=Q20_Negative_MS_Placenta_MSMS.mgf
SUBJECT_SAMPLE_FACTORS           	-	Blank_Positive_MS_UNC_1	Treatment:blank	RAW_FILE_NAME=Blank_Positive_MS_UNC_1.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Blank_Positive_MS_UNC_2	Treatment:blank	RAW_FILE_NAME=Blank_Positive_MS_UNC_2.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Blank_Positive_MS_UNC_3	Treatment:blank	RAW_FILE_NAME=Blank_Positive_MS_UNC_3.mzdata
SUBJECT_SAMPLE_FACTORS           	-	QC_Positive_MS_Pool_1	Treatment:QC	RAW_FILE_NAME=QC_Positive_MS_Pool_1.mzdata
SUBJECT_SAMPLE_FACTORS           	-	QC_Positive_MS_Pool_2	Treatment:QC	RAW_FILE_NAME=QC_Positive_MS_Pool_2.mzdata
SUBJECT_SAMPLE_FACTORS           	-	QC_Positive_MS_Pool_3	Treatment:QC	RAW_FILE_NAME=QC_Positive_MS_Pool_3.mzdata
SUBJECT_SAMPLE_FACTORS           	-	QC_Positive_MS_Pool_Front1	Treatment:QC	RAW_FILE_NAME=QC_Positive_MS_Pool_Front1.mzdata
SUBJECT_SAMPLE_FACTORS           	-	QC_Positive_MS_Pool_Front2	Treatment:QC	RAW_FILE_NAME=QC_Positive_MS_Pool_Front2.mzdata
SUBJECT_SAMPLE_FACTORS           	-	QC_Positive_MS_Pool_Front3	Treatment:QC	RAW_FILE_NAME=QC_Positive_MS_Pool_Front3.mzdata
SUBJECT_SAMPLE_FACTORS           	-	QC_Positive_MS_Pool_Front4	Treatment:QC	RAW_FILE_NAME=QC_Positive_MS_Pool_Front4.mzdata
SUBJECT_SAMPLE_FACTORS           	-	QC_Positive_MS_Pool_Front5	Treatment:QC	RAW_FILE_NAME=QC_Positive_MS_Pool_Front5.mzdata
SUBJECT_SAMPLE_FACTORS           	-	QC_Positive_MS_Pool_Mid1	Treatment:QC	RAW_FILE_NAME=QC_Positive_MS_Pool_Mid1.mzdata
SUBJECT_SAMPLE_FACTORS           	-	QC_Positive_MS_Pool_Mid2	Treatment:QC	RAW_FILE_NAME=QC_Positive_MS_Pool_Mid2.mzdata
SUBJECT_SAMPLE_FACTORS           	-	QC_Positive_MS_Pool_Mid3	Treatment:QC	RAW_FILE_NAME=QC_Positive_MS_Pool_Mid3.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Blank_Negative_MS_UNC_1	Treatment:blank	RAW_FILE_NAME=Blank_Negative_MS_UNC_1.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Blank_Negative_MS_UNC_2	Treatment:blank	RAW_FILE_NAME=Blank_Negative_MS_UNC_2.mzdata
SUBJECT_SAMPLE_FACTORS           	-	Blank_Negative_MS_UNC_3	Treatment:blank	RAW_FILE_NAME=Blank_Negative_MS_UNC_3.mzdata
SUBJECT_SAMPLE_FACTORS           	-	QC_Negative_MS_Pool_1	Treatment:QC	RAW_FILE_NAME=QC_Negative_MS_Pool_1.mzdata
SUBJECT_SAMPLE_FACTORS           	-	QC_Negative_MS_Pool_2	Treatment:QC	RAW_FILE_NAME=QC_Negative_MS_Pool_2.mzdata
SUBJECT_SAMPLE_FACTORS           	-	QC_Negative_MS_Pool_3	Treatment:QC	RAW_FILE_NAME=QC_Negative_MS_Pool_3.mzdata
SUBJECT_SAMPLE_FACTORS           	-	QC_Negative_MS_Pool_Front1	Treatment:QC	RAW_FILE_NAME=QC_Negative_MS_Pool_Front1.mzdata
SUBJECT_SAMPLE_FACTORS           	-	QC_Negative_MS_Pool_Front2	Treatment:QC	RAW_FILE_NAME=QC_Negative_MS_Pool_Front2.mzdata
SUBJECT_SAMPLE_FACTORS           	-	QC_Negative_MS_Pool_Front3	Treatment:QC	RAW_FILE_NAME=QC_Negative_MS_Pool_Front3.mzdata
SUBJECT_SAMPLE_FACTORS           	-	QC_Negative_MS_Pool_Front4	Treatment:QC	RAW_FILE_NAME=QC_Negative_MS_Pool_Front4.mzdata
SUBJECT_SAMPLE_FACTORS           	-	QC_Negative_MS_Pool_Front5	Treatment:QC	RAW_FILE_NAME=QC_Negative_MS_Pool_Front5.mzdata
SUBJECT_SAMPLE_FACTORS           	-	QC_Negative_MS_Pool_Mid1	Treatment:QC	RAW_FILE_NAME=QC_Negative_MS_Pool_Mid1.mzdata
SUBJECT_SAMPLE_FACTORS           	-	QC_Negative_MS_Pool_Mid2	Treatment:QC	RAW_FILE_NAME=QC_Negative_MS_Pool_Mid2.mzdata
SUBJECT_SAMPLE_FACTORS           	-	QC_Negative_MS_Pool_Mid3	Treatment:QC	RAW_FILE_NAME=QC_Negative_MS_Pool_Mid3.mzdata
#COLLECTION
CO:COLLECTION_SUMMARY            	Placentas were first processed by collecting samples ~4-6 inches in length and
CO:COLLECTION_SUMMARY            	½ inch in diameter via cross-section punch biopsy, with blinded identifiers to
CO:COLLECTION_SUMMARY            	ensure unbiased collection. These samples were stored at -80°C, until further
CO:COLLECTION_SUMMARY            	processing on dry ice into individual samples for chemical analyses. Here,
CO:COLLECTION_SUMMARY            	sterile scalpels were used to cut samples in half and a ¼ inch slice was
CO:COLLECTION_SUMMARY            	removed from the middle and weighed, yielding isolated samples ranging in weight
CO:COLLECTION_SUMMARY            	between 0.4 and 0.6 g.
CO:SAMPLE_TYPE                   	Placenta
#TREATMENT
TR:TREATMENT_SUMMARY             	Placentas were first processed by collecting samples ~4-6 inches in length and
TR:TREATMENT_SUMMARY             	½ inch in diameter via cross-section punch biopsy, with blinded identifiers to
TR:TREATMENT_SUMMARY             	ensure unbiased collection. These samples were stored at -80°C, until further
TR:TREATMENT_SUMMARY             	processing on dry ice into individual samples for chemical analyses. Here,
TR:TREATMENT_SUMMARY             	sterile scalpels were used to cut samples in half and a ¼ inch slice was
TR:TREATMENT_SUMMARY             	removed from the middle and weighed, yielding isolated samples ranging in weight
TR:TREATMENT_SUMMARY             	between 0.4 and 0.6 g.
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	One sample per tissue was used in chemical analyses. Chemicals were separated
SP:SAMPLEPREP_SUMMARY            	from tissue via solid-liquid extraction by adding 2 mL of room temperature
SP:SAMPLEPREP_SUMMARY            	water:acetonitrile (1:1) per sample. Placenta samples were disrupted and
SP:SAMPLEPREP_SUMMARY            	homogenized using a TissueRuptor (Qiagen), vortexed for 5 min, and placed in a
SP:SAMPLEPREP_SUMMARY            	centrifuge at 4000 rpm for 5 min. The resulting supernatant was collected, and a
SP:SAMPLEPREP_SUMMARY            	second round of solid-liquid extraction was performed using 8 mL acetonitrile
SP:SAMPLEPREP_SUMMARY            	with 2% formic acid. Samples were vortexed and centrifuged again, and both
SP:SAMPLEPREP_SUMMARY            	supernatants combined yielding a final total volume of 10 mL. Of the 10 mL
SP:SAMPLEPREP_SUMMARY            	combined supernatant sample, 3 mL were then run through solid filtration with a
SP:SAMPLEPREP_SUMMARY            	Captiva EMR-lipid column (Agilent Technologies). Resulting eluates were placed
SP:SAMPLEPREP_SUMMARY            	into liquid chromatography mass spectrometry (LCMS) vials in 1.0 mL aliquots.
SP:SAMPLEPREP_SUMMARY            	Remaining supernatant volumes were banked at -80°C. Method blanks were
SP:SAMPLEPREP_SUMMARY            	collected in parallel using the same steps without adding tissues. For
SP:SAMPLEPREP_SUMMARY            	non-targeted chemical analysis, 20 µL of 1.25 µg/mL tracer solution was added
SP:SAMPLEPREP_SUMMARY            	to each 1.0 mL aliquot of post-filtration eluate (for study samples, blanks, and
SP:SAMPLEPREP_SUMMARY            	controls) yielding a final concentration of 25 ng/mL per sample. In addition,
SP:SAMPLEPREP_SUMMARY            	tracers were added to a single sample in duplicate prior to extraction to
SP:SAMPLEPREP_SUMMARY            	evaluate extraction recovery and reproducibility.
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_TYPE           	Reversed phase
CH:INSTRUMENT_NAME               	Agilent 1290 Infinity II
CH:COLUMN_NAME                   	Agilent Zorbax Eclipse Plus C8 (2.1 x 100 mm, 1.8 µm) UHPLC column
#ANALYSIS
AN:ANALYSIS_TYPE                 	MS
#MS
MS:INSTRUMENT_NAME               	Agilent 6545 QTOF
MS:INSTRUMENT_TYPE               	QTOF
MS:MS_TYPE                       	ESI
MS:ION_MODE                      	UNSPECIFIED
MS:MS_COMMENTS                   	See Acquisition_Methods.docx for more comprehensive acquisition parameters
MS:MS_RESULTS_FILE               	ST002151_AN003522_Results.txt	UNITS:amu	Has m/z:No	Has RT:No	RT units:No RT data
#END