#METABOLOMICS WORKBENCH Poonam27_20211129_021911 DATATRACK_ID:2952 STUDY_ID:ST002166 ANALYSIS_ID:AN003549 PROJECT_ID:PR001378
VERSION             	1
CREATED_ON             	November 29, 2021, 3:43 am
#PROJECT
PR:PROJECT_TITLE                 	Untargeted metabolomics following radiation exposure
PR:PROJECT_TYPE                  	Untargeted metabolic profiling
PR:PROJECT_SUMMARY               	Comprehensive metabolomics profiling of mice urine after 5 Gy and 7.5 Gy total
PR:PROJECT_SUMMARY               	body radiation exposure at 24 hours using LC-MS and NMR.
PR:INSTITUTE                     	Institute of Nuclear Medicine and Allied Sciences
PR:DEPARTMENT                    	Metabolomics facility
PR:LABORATORY                    	Metabolomics facility
PR:LAST_NAME                     	Rana
PR:FIRST_NAME                    	Poonam
PR:ADDRESS                       	DRDO, Lucknow Road, Timarpur, Delhi, Delhi, 110054, India
PR:EMAIL                         	poonam@inmas.drdo.in
PR:PHONE                         	+911123905319
#STUDY
ST:STUDY_TITLE                   	Untargeted metabolomics profiling of mice urine after 5 Gy and 7.5 Gy total body
ST:STUDY_TITLE                   	radiation exposure at 24 hours.
ST:STUDY_TYPE                    	Untargeted metabolic profiling
ST:STUDY_SUMMARY                 	Comprehensive metabolomics profiling of mice urine after 5 Gy and 7.5 Gy total
ST:STUDY_SUMMARY                 	body radiation exposure at 24 hours
ST:INSTITUTE                     	Institute of Nuclear Medicine and Allied Sciences
ST:LAST_NAME                     	Rana
ST:FIRST_NAME                    	Poonam
ST:ADDRESS                       	DRDO, Lucknow Road, Timarpur, Delhi, Delhi, 110054, India
ST:EMAIL                         	poonam@inmas.drdo.in
ST:PHONE                         	+911123905319
ST:NUM_GROUPS                    	3
ST:TOTAL_SUBJECTS                	21
ST:NUM_MALES                     	21
#SUBJECT
SU:SUBJECT_TYPE                  	Other organism
SU:SUBJECT_SPECIES               	Mus musculus
SU:TAXONOMY_ID                   	10090
SU:GENDER                        	Male
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data
SUBJECT_SAMPLE_FACTORS           	-	R_Ctrl 1	Treatment:Control	
SUBJECT_SAMPLE_FACTORS           	-	R_Ctrl 2	Treatment:Control	
SUBJECT_SAMPLE_FACTORS           	-	R_Ctrl 3	Treatment:Control	
SUBJECT_SAMPLE_FACTORS           	-	R_Ctrl 4	Treatment:Control	
SUBJECT_SAMPLE_FACTORS           	-	R_Ctrl 5	Treatment:Control	
SUBJECT_SAMPLE_FACTORS           	-	R_Ctrl 6	Treatment:Control	
SUBJECT_SAMPLE_FACTORS           	-	R_Ctrl 7	Treatment:Control	
SUBJECT_SAMPLE_FACTORS           	-	R_5 Gy 1	Treatment:5Gy Irradiated	
SUBJECT_SAMPLE_FACTORS           	-	R_5 Gy 2	Treatment:5Gy Irradiated	
SUBJECT_SAMPLE_FACTORS           	-	R_5 Gy 3	Treatment:5Gy Irradiated	
SUBJECT_SAMPLE_FACTORS           	-	R_5 Gy 4	Treatment:5Gy Irradiated	
SUBJECT_SAMPLE_FACTORS           	-	R_5 Gy 5	Treatment:5Gy Irradiated	
SUBJECT_SAMPLE_FACTORS           	-	R_5 Gy 6	Treatment:5Gy Irradiated	
SUBJECT_SAMPLE_FACTORS           	-	R_5 Gy 7	Treatment:5Gy Irradiated	
SUBJECT_SAMPLE_FACTORS           	-	R_7.5 Gy 1	Treatment:7.5Gy Irradiated	
SUBJECT_SAMPLE_FACTORS           	-	R_7.5 Gy 2	Treatment:7.5Gy Irradiated	
SUBJECT_SAMPLE_FACTORS           	-	R_7.5 Gy 3	Treatment:7.5Gy Irradiated	
SUBJECT_SAMPLE_FACTORS           	-	R_7.5 Gy 4	Treatment:7.5Gy Irradiated	
SUBJECT_SAMPLE_FACTORS           	-	R_7.5 Gy 6	Treatment:7.5Gy Irradiated	
SUBJECT_SAMPLE_FACTORS           	-	R_7.5 Gy 7	Treatment:7.5Gy Irradiated	
#COLLECTION
CO:COLLECTION_SUMMARY            	Animals were acclimatized for 48 hrs in polypropylene cages. Urine samples were
CO:COLLECTION_SUMMARY            	collected at 24 hrs post radiation exposure.
CO:SAMPLE_TYPE                   	Urine
CO:STORAGE_CONDITIONS            	-80℃
CO:COLLECTION_TUBE_TEMP          	4
#TREATMENT
TR:TREATMENT_SUMMARY             	Random categorization was done into three groups: control, 5 Gy (sub-lethal) and
TR:TREATMENT_SUMMARY             	7.5 Gy (lethal) irradiated (seven animals in each group). Mice were kept for 12
TR:TREATMENT_SUMMARY             	hrs light and dark cycle with room temperature (19–23°C) and humidity
TR:TREATMENT_SUMMARY             	(45–65%) conditions properly maintained. Mice were exposed to total-body gamma
TR:TREATMENT_SUMMARY             	radiation (field size; 35x35 cm) of 5 Gy and 7.5 Gy through Tele 60Co unit γ-
TR:TREATMENT_SUMMARY             	irradiation facility (Bhabhatron II, Panacea Medical, Bangalore, India) with
TR:TREATMENT_SUMMARY             	dose rate of 1 Gy/min. Control mice were sham irradiated.
TR:TREATMENT                     	Radiation exposure
TR:TREATMENT_DOSE                	5 Gy and 7.5 Gy Gamma-60Cobalt irradiation
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	150 µl of urine was mixed with 375 µl of deuterated phosphate buffer solution
SP:SAMPLEPREP_SUMMARY            	(pH 7.2- 7.4) containing 0.5 mM TSP prepared in D2O. The solution was then
SP:SAMPLEPREP_SUMMARY            	transferred to 5 mm NMR tube for data acquisition
#ANALYSIS
AN:ANALYSIS_TYPE                 	NMR
#NMR
NM:INSTRUMENT_NAME               	Bruker
NM:INSTRUMENT_TYPE               	FT-NMR
NM:NMR_EXPERIMENT_TYPE           	1D-1H
NM:SPECTROMETER_FREQUENCY        	600MHz
NM:NMR_RESULTS_FILE               	ST002166_AN003549_Results.txt	UNITS:Integral
#END