#METABOLOMICS WORKBENCH annakisiala_20221017_123232 DATATRACK_ID:3515 STUDY_ID:ST002330 ANALYSIS_ID:AN003802 PROJECT_ID:PR001494
VERSION             	1
CREATED_ON             	November 2, 2022, 8:12 am
#PROJECT
PR:PROJECT_TITLE                 	Early-stage responses to Plasmodiophora brassicae at the metabolome levels in
PR:PROJECT_TITLE                 	clubroot resistant and susceptible oilseed Brassica napus
PR:PROJECT_TYPE                  	MS quantitative analysis
PR:PROJECT_SUMMARY               	Clubroot, a devastating soil-borne root disease, in Brassicaceae is caused by
PR:PROJECT_SUMMARY               	Plasmodiophora brassicae Woronin, an obligate biotrophic protist. Plant growth
PR:PROJECT_SUMMARY               	and development, as well as seed yield of Brassica crops, are severely affected
PR:PROJECT_SUMMARY               	due to this disease. Several reports described the molecular responses of B.
PR:PROJECT_SUMMARY               	napus to P. brassicae; however, information on the early stages of pathogenesis
PR:PROJECT_SUMMARY               	is limited. In this study, we have used metabolomics approach to characterize P.
PR:PROJECT_SUMMARY               	brassicae pathogenesis at 1-, 4-, and 7- days post-inoculation (DPI) in clubroot
PR:PROJECT_SUMMARY               	resistant (CR) and susceptible (CS) doubled-haploid (DH) lines. Several
PR:PROJECT_SUMMARY               	metabolites related to organic acids (e.g., citrate, pyruvate), amino acids
PR:PROJECT_SUMMARY               	(e.g., proline, aspartate), sugars, and mannitol, were differentially
PR:PROJECT_SUMMARY               	accumulated in roots in response to pathogen infection when the CR and CS
PR:PROJECT_SUMMARY               	genotypes were compared. Our results suggest important roles for these
PR:PROJECT_SUMMARY               	metabolites in mediating resistance to clubroot disease. To our knowledge, this
PR:PROJECT_SUMMARY               	is the first report of an integrated metabolome analysis aimed at characterizing
PR:PROJECT_SUMMARY               	the molecular basis of resistance to clubroot in canola.
PR:INSTITUTE                     	Trent University
PR:DEPARTMENT                    	Biology
PR:LABORATORY                    	Emery Lab
PR:LAST_NAME                     	Kisiala
PR:FIRST_NAME                    	Anna
PR:ADDRESS                       	1600 West Bank Drive, Trent University, Peterborough, ON, K9L 0G2, Canada
PR:EMAIL                         	annakisiala@trentu.ca
PR:PHONE                         	7057481011
PR:FUNDING_SOURCE                	Alberta Agriculture and Forestry
PR:CONTRIBUTORS                  	Dinesh Adhikary, Anna Kisiala, Ananya Sarkar, Urmila Basu, Habibur Rahman, Neil
PR:CONTRIBUTORS                  	Emery, Nat N. V. Kav
#STUDY
ST:STUDY_TITLE                   	Early-stage responses to Plasmodiophora brassicae at the metabolome levels in
ST:STUDY_TITLE                   	clubroot resistant and susceptible oilseed Brassica napus
ST:STUDY_TYPE                    	Timecourse experiment
ST:STUDY_SUMMARY                 	A total of 36 samples comprised two types of genotypes [CR (5 individuals pooled
ST:STUDY_SUMMARY                 	in each biological replicate) and CS (5 individuals pooled in each biological
ST:STUDY_SUMMARY                 	replicate)], two treatments (inoculated and uninoculated) and three biological
ST:STUDY_SUMMARY                 	replicates generated from three independent experiments and collected at 1-, 4-,
ST:STUDY_SUMMARY                 	and 7–DPI were used to extract primary and secondary metabolites and analyse
ST:STUDY_SUMMARY                 	the differences among the treatments.
ST:INSTITUTE                     	Trent University
ST:DEPARTMENT                    	Biology
ST:LABORATORY                    	Emery Lab
ST:LAST_NAME                     	Kisiala
ST:FIRST_NAME                    	Anna
ST:ADDRESS                       	1600 West Bank Drive, Trent University
ST:EMAIL                         	annakisiala@trentu.ca
ST:PHONE                         	7057481011
#SUBJECT
SU:SUBJECT_TYPE                  	Plant
SU:SUBJECT_SPECIES               	Brassica napus
SU:TAXONOMY_ID                   	3708
#FACTORS
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data
SUBJECT_SAMPLE_FACTORS           	-	C18-RN1-pos	Developmental stage (DPI):day 1 | Genotype:Resistant | Treatment:Control	RAW_FILE_NAME=C18-RN1-pos.mzXML
SUBJECT_SAMPLE_FACTORS           	-	C18-RN1-neg	Developmental stage (DPI):day 1 | Genotype:Resistant | Treatment:Control	RAW_FILE_NAME=C18-RN1-neg.mzXML
SUBJECT_SAMPLE_FACTORS           	-	HILIC-RN1-pos	Developmental stage (DPI):day 1 | Genotype:Resistant | Treatment:Control	RAW_FILE_NAME=HILIC-RN1-pos.mzXML
SUBJECT_SAMPLE_FACTORS           	-	HILIC-RN1-neg	Developmental stage (DPI):day 1 | Genotype:Resistant | Treatment:Control	RAW_FILE_NAME=HILIC-RN1-neg.mzXML
SUBJECT_SAMPLE_FACTORS           	-	C18-RN2-pos	Developmental stage (DPI):day 1 | Genotype:Resistant | Treatment:Control	RAW_FILE_NAME=C18-RN2-pos.mzXML
SUBJECT_SAMPLE_FACTORS           	-	C18-RN2-neg	Developmental stage (DPI):day 1 | Genotype:Resistant | Treatment:Control	RAW_FILE_NAME=C18-RN2-neg.mzXML
SUBJECT_SAMPLE_FACTORS           	-	HILIC-RN2-pos	Developmental stage (DPI):day 1 | Genotype:Resistant | Treatment:Control	RAW_FILE_NAME=HILIC-RN2-pos.mzXML
SUBJECT_SAMPLE_FACTORS           	-	HILIC-RN2-neg	Developmental stage (DPI):day 1 | Genotype:Resistant | Treatment:Control	RAW_FILE_NAME=HILIC-RN2-neg.mzXML
SUBJECT_SAMPLE_FACTORS           	-	C18-RN3-pos	Developmental stage (DPI):day 1 | Genotype:Resistant | Treatment:Control	RAW_FILE_NAME=C18-RN3-pos.mzXML
SUBJECT_SAMPLE_FACTORS           	-	C18-RN3-neg	Developmental stage (DPI):day 1 | Genotype:Resistant | Treatment:Control	RAW_FILE_NAME=C18-RN3-neg.mzXML
SUBJECT_SAMPLE_FACTORS           	-	HILIC-RN3-pos	Developmental stage (DPI):day 1 | Genotype:Resistant | Treatment:Control	RAW_FILE_NAME=HILIC-RN3-pos.mzXML
SUBJECT_SAMPLE_FACTORS           	-	HILIC-RN3-neg	Developmental stage (DPI):day 1 | Genotype:Resistant | Treatment:Control	RAW_FILE_NAME=HILIC-RN3-neg.mzXML
SUBJECT_SAMPLE_FACTORS           	-	C18-RN4-pos	Developmental stage (DPI):day 1 | Genotype:Resistant | Treatment:Treated with Plasmodiophora brassicae	RAW_FILE_NAME=C18-RN4-pos.mzXML
SUBJECT_SAMPLE_FACTORS           	-	C18-RN4-neg	Developmental stage (DPI):day 1 | Genotype:Resistant | Treatment:Treated with Plasmodiophora brassicae	RAW_FILE_NAME=C18-RN4-neg.mzXML
SUBJECT_SAMPLE_FACTORS           	-	HILIC-RN4-pos	Developmental stage (DPI):day 1 | Genotype:Resistant | Treatment:Treated with Plasmodiophora brassicae	RAW_FILE_NAME=HILIC-RN4-pos.mzXML
SUBJECT_SAMPLE_FACTORS           	-	HILIC-RN4-neg	Developmental stage (DPI):day 1 | Genotype:Resistant | Treatment:Treated with Plasmodiophora brassicae	RAW_FILE_NAME=HILIC-RN4-neg.mzXML
SUBJECT_SAMPLE_FACTORS           	-	C18-RN5-pos	Developmental stage (DPI):day 1 | Genotype:Resistant | Treatment:Treated with Plasmodiophora brassicae	RAW_FILE_NAME=C18-RN5-pos.mzXML
SUBJECT_SAMPLE_FACTORS           	-	C18-RN5-neg	Developmental stage (DPI):day 1 | Genotype:Resistant | Treatment:Treated with Plasmodiophora brassicae	RAW_FILE_NAME=C18-RN5-neg.mzXML
SUBJECT_SAMPLE_FACTORS           	-	HILIC-RN5-pos	Developmental stage (DPI):day 1 | Genotype:Resistant | Treatment:Treated with Plasmodiophora brassicae	RAW_FILE_NAME=HILIC-RN5-pos.mzXML
SUBJECT_SAMPLE_FACTORS           	-	HILIC-RN5-neg	Developmental stage (DPI):day 1 | Genotype:Resistant | Treatment:Treated with Plasmodiophora brassicae	RAW_FILE_NAME=HILIC-RN5-neg.mzXML
SUBJECT_SAMPLE_FACTORS           	-	C18-RN6-pos	Developmental stage (DPI):day 1 | Genotype:Resistant | Treatment:Treated with Plasmodiophora brassicae	RAW_FILE_NAME=C18-RN6-pos.mzXML
SUBJECT_SAMPLE_FACTORS           	-	C18-RN6-neg	Developmental stage (DPI):day 1 | Genotype:Resistant | Treatment:Treated with Plasmodiophora brassicae	RAW_FILE_NAME=C18-RN6-neg.mzXML
SUBJECT_SAMPLE_FACTORS           	-	HILIC-RN6-pos	Developmental stage (DPI):day 1 | Genotype:Resistant | Treatment:Treated with Plasmodiophora brassicae	RAW_FILE_NAME=HILIC-RN6-pos.mzXML
SUBJECT_SAMPLE_FACTORS           	-	HILIC-RN6-neg	Developmental stage (DPI):day 1 | Genotype:Resistant | Treatment:Treated with Plasmodiophora brassicae	RAW_FILE_NAME=HILIC-RN6-neg.mzXML
SUBJECT_SAMPLE_FACTORS           	-	C18-RN7-pos	Developmental stage (DPI):day 1 | Genotype:Susceptible | Treatment:Control	RAW_FILE_NAME=C18-RN7-pos.mzXML
SUBJECT_SAMPLE_FACTORS           	-	C18-RN7-neg	Developmental stage (DPI):day 1 | Genotype:Susceptible | Treatment:Control	RAW_FILE_NAME=C18-RN7-neg.mzXML
SUBJECT_SAMPLE_FACTORS           	-	HILIC-RN7-pos	Developmental stage (DPI):day 1 | Genotype:Susceptible | Treatment:Control	RAW_FILE_NAME=HILIC-RN7-pos.mzXML
SUBJECT_SAMPLE_FACTORS           	-	HILIC-RN7-neg	Developmental stage (DPI):day 1 | Genotype:Susceptible | Treatment:Control	RAW_FILE_NAME=HILIC-RN7-neg.mzXML
SUBJECT_SAMPLE_FACTORS           	-	C18-RN8-pos	Developmental stage (DPI):day 1 | Genotype:Susceptible | Treatment:Control	RAW_FILE_NAME=C18-RN8-pos.mzXML
SUBJECT_SAMPLE_FACTORS           	-	C18-RN8-neg	Developmental stage (DPI):day 1 | Genotype:Susceptible | Treatment:Control	RAW_FILE_NAME=C18-RN8-neg.mzXML
SUBJECT_SAMPLE_FACTORS           	-	HILIC-RN8-pos	Developmental stage (DPI):day 1 | Genotype:Susceptible | Treatment:Control	RAW_FILE_NAME=HILIC-RN8-pos.mzXML
SUBJECT_SAMPLE_FACTORS           	-	HILIC-RN8-neg	Developmental stage (DPI):day 1 | Genotype:Susceptible | Treatment:Control	RAW_FILE_NAME=HILIC-RN8-neg.mzXML
SUBJECT_SAMPLE_FACTORS           	-	C18-RN9-pos	Developmental stage (DPI):day 1 | Genotype:Susceptible | Treatment:Control	RAW_FILE_NAME=C18-RN9-pos.mzXML
SUBJECT_SAMPLE_FACTORS           	-	C18-RN9-neg	Developmental stage (DPI):day 1 | Genotype:Susceptible | Treatment:Control	RAW_FILE_NAME=C18-RN9-neg.mzXML
SUBJECT_SAMPLE_FACTORS           	-	HILIC-RN9-pos	Developmental stage (DPI):day 1 | Genotype:Susceptible | Treatment:Control	RAW_FILE_NAME=HILIC-RN9-pos.mzXML
SUBJECT_SAMPLE_FACTORS           	-	HILIC-RN9-neg	Developmental stage (DPI):day 1 | Genotype:Susceptible | Treatment:Control	RAW_FILE_NAME=HILIC-RN9-neg.mzXML
SUBJECT_SAMPLE_FACTORS           	-	C18-RN10-pos	Developmental stage (DPI):day 1 | Genotype:Susceptible | Treatment:Treated with Plasmodiophora brassicae	RAW_FILE_NAME=C18-RN10-pos.mzXML
SUBJECT_SAMPLE_FACTORS           	-	C18-RN10-neg	Developmental stage (DPI):day 1 | Genotype:Susceptible | Treatment:Treated with Plasmodiophora brassicae	RAW_FILE_NAME=C18-RN10-neg.mzXML
SUBJECT_SAMPLE_FACTORS           	-	HILIC-RN10-pos	Developmental stage (DPI):day 1 | Genotype:Susceptible | Treatment:Treated with Plasmodiophora brassicae	RAW_FILE_NAME=HILIC-RN10-pos.mzXML
SUBJECT_SAMPLE_FACTORS           	-	HILIC-RN10-neg	Developmental stage (DPI):day 1 | Genotype:Susceptible | Treatment:Treated with Plasmodiophora brassicae	RAW_FILE_NAME=HILIC-RN10-neg.mzXML
SUBJECT_SAMPLE_FACTORS           	-	C18-RN11-pos	Developmental stage (DPI):day 1 | Genotype:Susceptible | Treatment:Treated with Plasmodiophora brassicae	RAW_FILE_NAME=C18-RN11-pos.mzXML
SUBJECT_SAMPLE_FACTORS           	-	C18-RN11-neg	Developmental stage (DPI):day 1 | Genotype:Susceptible | Treatment:Treated with Plasmodiophora brassicae	RAW_FILE_NAME=C18-RN11-neg.mzXML
SUBJECT_SAMPLE_FACTORS           	-	HILIC-RN11-pos	Developmental stage (DPI):day 1 | Genotype:Susceptible | Treatment:Treated with Plasmodiophora brassicae	RAW_FILE_NAME=HILIC-RN11-pos.mzXML
SUBJECT_SAMPLE_FACTORS           	-	HILIC-RN11-neg	Developmental stage (DPI):day 1 | Genotype:Susceptible | Treatment:Treated with Plasmodiophora brassicae	RAW_FILE_NAME=HILIC-RN11-neg.mzXML
SUBJECT_SAMPLE_FACTORS           	-	C18-RN12-pos	Developmental stage (DPI):day 1 | Genotype:Susceptible | Treatment:Treated with Plasmodiophora brassicae	RAW_FILE_NAME=C18-RN12-pos.mzXML
SUBJECT_SAMPLE_FACTORS           	-	C18-RN12-neg	Developmental stage (DPI):day 1 | Genotype:Susceptible | Treatment:Treated with Plasmodiophora brassicae	RAW_FILE_NAME=C18-RN12-neg.mzXML
SUBJECT_SAMPLE_FACTORS           	-	HILIC-RN12-pos	Developmental stage (DPI):day 1 | Genotype:Susceptible | Treatment:Treated with Plasmodiophora brassicae	RAW_FILE_NAME=HILIC-RN12-pos.mzXML
SUBJECT_SAMPLE_FACTORS           	-	HILIC-RN12-neg	Developmental stage (DPI):day 1 | Genotype:Susceptible | Treatment:Treated with Plasmodiophora brassicae	RAW_FILE_NAME=HILIC-RN12-neg.mzXML
SUBJECT_SAMPLE_FACTORS           	-	C18-RN13-pos	Developmental stage (DPI):day 4 | Genotype:Resistant | Treatment:Control	RAW_FILE_NAME=C18-RN13-pos.mzXML
SUBJECT_SAMPLE_FACTORS           	-	C18-RN13-neg	Developmental stage (DPI):day 4 | Genotype:Resistant | Treatment:Control	RAW_FILE_NAME=C18-RN13-neg.mzXML
SUBJECT_SAMPLE_FACTORS           	-	HILIC-RN13-pos	Developmental stage (DPI):day 4 | Genotype:Resistant | Treatment:Control	RAW_FILE_NAME=HILIC-RN13-pos.mzXML
SUBJECT_SAMPLE_FACTORS           	-	HILIC-RN13-neg	Developmental stage (DPI):day 4 | Genotype:Resistant | Treatment:Control	RAW_FILE_NAME=HILIC-RN13-neg.mzXML
SUBJECT_SAMPLE_FACTORS           	-	C18-RN14-pos	Developmental stage (DPI):day 4 | Genotype:Resistant | Treatment:Control	RAW_FILE_NAME=C18-RN14-pos.mzXML
SUBJECT_SAMPLE_FACTORS           	-	C18-RN14-neg	Developmental stage (DPI):day 4 | Genotype:Resistant | Treatment:Control	RAW_FILE_NAME=C18-RN14-neg.mzXML
SUBJECT_SAMPLE_FACTORS           	-	HILIC-RN14-pos	Developmental stage (DPI):day 4 | Genotype:Resistant | Treatment:Control	RAW_FILE_NAME=HILIC-RN14-pos.mzXML
SUBJECT_SAMPLE_FACTORS           	-	HILIC-RN14-neg	Developmental stage (DPI):day 4 | Genotype:Resistant | Treatment:Control	RAW_FILE_NAME=HILIC-RN14-neg.mzXML
SUBJECT_SAMPLE_FACTORS           	-	C18-RN15-pos	Developmental stage (DPI):day 4 | Genotype:Resistant | Treatment:Control	RAW_FILE_NAME=C18-RN15-pos.mzXML
SUBJECT_SAMPLE_FACTORS           	-	C18-RN15-neg	Developmental stage (DPI):day 4 | Genotype:Resistant | Treatment:Control	RAW_FILE_NAME=C18-RN15-neg.mzXML
SUBJECT_SAMPLE_FACTORS           	-	HILIC-RN15-pos	Developmental stage (DPI):day 4 | Genotype:Resistant | Treatment:Control	RAW_FILE_NAME=HILIC-RN15-pos.mzXML
SUBJECT_SAMPLE_FACTORS           	-	HILIC-RN15-neg	Developmental stage (DPI):day 4 | Genotype:Resistant | Treatment:Control	RAW_FILE_NAME=HILIC-RN15-neg.mzXML
SUBJECT_SAMPLE_FACTORS           	-	C18-RN16-pos	Developmental stage (DPI):day 4 | Genotype:Resistant | Treatment:Treated with Plasmodiophora brassicae	RAW_FILE_NAME=C18-RN16-pos.mzXML
SUBJECT_SAMPLE_FACTORS           	-	C18-RN16-neg	Developmental stage (DPI):day 4 | Genotype:Resistant | Treatment:Treated with Plasmodiophora brassicae	RAW_FILE_NAME=C18-RN16-neg.mzXML
SUBJECT_SAMPLE_FACTORS           	-	HILIC-RN16-pos	Developmental stage (DPI):day 4 | Genotype:Resistant | Treatment:Treated with Plasmodiophora brassicae	RAW_FILE_NAME=HILIC-RN16-pos.mzXML
SUBJECT_SAMPLE_FACTORS           	-	HILIC-RN16-neg	Developmental stage (DPI):day 4 | Genotype:Resistant | Treatment:Treated with Plasmodiophora brassicae	RAW_FILE_NAME=HILIC-RN16-neg.mzXML
SUBJECT_SAMPLE_FACTORS           	-	C18-RN17-pos	Developmental stage (DPI):day 4 | Genotype:Resistant | Treatment:Treated with Plasmodiophora brassicae	RAW_FILE_NAME=C18-RN17-pos.mzXML
SUBJECT_SAMPLE_FACTORS           	-	C18-RN17-neg	Developmental stage (DPI):day 4 | Genotype:Resistant | Treatment:Treated with Plasmodiophora brassicae	RAW_FILE_NAME=C18-RN17-neg.mzXML
SUBJECT_SAMPLE_FACTORS           	-	HILIC-RN17-pos	Developmental stage (DPI):day 4 | Genotype:Resistant | Treatment:Treated with Plasmodiophora brassicae	RAW_FILE_NAME=HILIC-RN17-pos.mzXML
SUBJECT_SAMPLE_FACTORS           	-	HILIC-RN17-neg	Developmental stage (DPI):day 4 | Genotype:Resistant | Treatment:Treated with Plasmodiophora brassicae	RAW_FILE_NAME=HILIC-RN17-neg.mzXML
SUBJECT_SAMPLE_FACTORS           	-	C18-RN18-pos	Developmental stage (DPI):day 4 | Genotype:Resistant | Treatment:Treated with Plasmodiophora brassicae	RAW_FILE_NAME=C18-RN18-pos.mzXML
SUBJECT_SAMPLE_FACTORS           	-	C18-RN18-neg	Developmental stage (DPI):day 4 | Genotype:Resistant | Treatment:Treated with Plasmodiophora brassicae	RAW_FILE_NAME=C18-RN18-neg.mzXML
SUBJECT_SAMPLE_FACTORS           	-	HILIC-RN18-pos	Developmental stage (DPI):day 4 | Genotype:Resistant | Treatment:Treated with Plasmodiophora brassicae	RAW_FILE_NAME=HILIC-RN18-pos.mzXML
SUBJECT_SAMPLE_FACTORS           	-	HILIC-RN18-neg	Developmental stage (DPI):day 4 | Genotype:Resistant | Treatment:Treated with Plasmodiophora brassicae	RAW_FILE_NAME=HILIC-RN18-neg.mzXML
SUBJECT_SAMPLE_FACTORS           	-	C18-RN19-pos	Developmental stage (DPI):day 4 | Genotype:Susceptible | Treatment:Control	RAW_FILE_NAME=C18-RN19-pos.mzXML
SUBJECT_SAMPLE_FACTORS           	-	C18-RN19-neg	Developmental stage (DPI):day 4 | Genotype:Susceptible | Treatment:Control	RAW_FILE_NAME=C18-RN19-neg.mzXML
SUBJECT_SAMPLE_FACTORS           	-	HILIC-RN19-pos	Developmental stage (DPI):day 4 | Genotype:Susceptible | Treatment:Control	RAW_FILE_NAME=HILIC-RN19-pos.mzXML
SUBJECT_SAMPLE_FACTORS           	-	HILIC-RN19-neg	Developmental stage (DPI):day 4 | Genotype:Susceptible | Treatment:Control	RAW_FILE_NAME=HILIC-RN19-neg.mzXML
SUBJECT_SAMPLE_FACTORS           	-	C18-RN20-pos	Developmental stage (DPI):day 4 | Genotype:Susceptible | Treatment:Control	RAW_FILE_NAME=C18-RN20-pos.mzXML
SUBJECT_SAMPLE_FACTORS           	-	C18-RN20-neg	Developmental stage (DPI):day 4 | Genotype:Susceptible | Treatment:Control	RAW_FILE_NAME=C18-RN20-neg.mzXML
SUBJECT_SAMPLE_FACTORS           	-	HILIC-RN20-pos	Developmental stage (DPI):day 4 | Genotype:Susceptible | Treatment:Control	RAW_FILE_NAME=HILIC-RN20-pos.mzXML
SUBJECT_SAMPLE_FACTORS           	-	HILIC-RN20-neg	Developmental stage (DPI):day 4 | Genotype:Susceptible | Treatment:Control	RAW_FILE_NAME=HILIC-RN20-neg.mzXML
SUBJECT_SAMPLE_FACTORS           	-	C18-RN21-pos	Developmental stage (DPI):day 4 | Genotype:Susceptible | Treatment:Control	RAW_FILE_NAME=C18-RN21-pos.mzXML
SUBJECT_SAMPLE_FACTORS           	-	C18-RN21-neg	Developmental stage (DPI):day 4 | Genotype:Susceptible | Treatment:Control	RAW_FILE_NAME=C18-RN21-neg.mzXML
SUBJECT_SAMPLE_FACTORS           	-	HILIC-RN21-pos	Developmental stage (DPI):day 4 | Genotype:Susceptible | Treatment:Control	RAW_FILE_NAME=HILIC-RN21-pos.mzXML
SUBJECT_SAMPLE_FACTORS           	-	HILIC-RN21-neg	Developmental stage (DPI):day 4 | Genotype:Susceptible | Treatment:Control	RAW_FILE_NAME=HILIC-RN21-neg.mzXML
SUBJECT_SAMPLE_FACTORS           	-	C18-RN22-pos	Developmental stage (DPI):day 4 | Genotype:Susceptible | Treatment:Treated with Plasmodiophora brassicae	RAW_FILE_NAME=C18-RN22-pos.mzXML
SUBJECT_SAMPLE_FACTORS           	-	C18-RN22-neg	Developmental stage (DPI):day 4 | Genotype:Susceptible | Treatment:Treated with Plasmodiophora brassicae	RAW_FILE_NAME=C18-RN22-neg.mzXML
SUBJECT_SAMPLE_FACTORS           	-	HILIC-RN22-pos	Developmental stage (DPI):day 4 | Genotype:Susceptible | Treatment:Treated with Plasmodiophora brassicae	RAW_FILE_NAME=HILIC-RN22-pos.mzXML
SUBJECT_SAMPLE_FACTORS           	-	HILIC-RN22-neg	Developmental stage (DPI):day 4 | Genotype:Susceptible | Treatment:Treated with Plasmodiophora brassicae	RAW_FILE_NAME=HILIC-RN22-neg.mzXML
SUBJECT_SAMPLE_FACTORS           	-	C18-RN23-pos	Developmental stage (DPI):day 4 | Genotype:Susceptible | Treatment:Treated with Plasmodiophora brassicae	RAW_FILE_NAME=C18-RN23-pos.mzXML
SUBJECT_SAMPLE_FACTORS           	-	C18-RN23-neg	Developmental stage (DPI):day 4 | Genotype:Susceptible | Treatment:Treated with Plasmodiophora brassicae	RAW_FILE_NAME=C18-RN23-neg.mzXML
SUBJECT_SAMPLE_FACTORS           	-	HILIC-RN23-pos	Developmental stage (DPI):day 4 | Genotype:Susceptible | Treatment:Treated with Plasmodiophora brassicae	RAW_FILE_NAME=HILIC-RN23-pos.mzXML
SUBJECT_SAMPLE_FACTORS           	-	HILIC-RN23-neg	Developmental stage (DPI):day 4 | Genotype:Susceptible | Treatment:Treated with Plasmodiophora brassicae	RAW_FILE_NAME=HILIC-RN23-neg.mzXML
SUBJECT_SAMPLE_FACTORS           	-	C18-RN24-pos	Developmental stage (DPI):day 4 | Genotype:Susceptible | Treatment:Treated with Plasmodiophora brassicae	RAW_FILE_NAME=C18-RN24-pos.mzXML
SUBJECT_SAMPLE_FACTORS           	-	C18-RN24-neg	Developmental stage (DPI):day 4 | Genotype:Susceptible | Treatment:Treated with Plasmodiophora brassicae	RAW_FILE_NAME=C18-RN24-neg.mzXML
SUBJECT_SAMPLE_FACTORS           	-	HILIC-RN24-pos	Developmental stage (DPI):day 4 | Genotype:Susceptible | Treatment:Treated with Plasmodiophora brassicae	RAW_FILE_NAME=HILIC-RN24-pos.mzXML
SUBJECT_SAMPLE_FACTORS           	-	HILIC-RN24-neg	Developmental stage (DPI):day 4 | Genotype:Susceptible | Treatment:Treated with Plasmodiophora brassicae	RAW_FILE_NAME=HILIC-RN24-neg.mzXML
SUBJECT_SAMPLE_FACTORS           	-	C18-RN25-pos	Developmental stage (DPI):day 7 | Genotype:Resistant | Treatment:Control	RAW_FILE_NAME=C18-RN25-pos.mzXML
SUBJECT_SAMPLE_FACTORS           	-	C18-RN25-neg	Developmental stage (DPI):day 7 | Genotype:Resistant | Treatment:Control	RAW_FILE_NAME=C18-RN25-neg.mzXML
SUBJECT_SAMPLE_FACTORS           	-	HILIC-RN25-pos	Developmental stage (DPI):day 7 | Genotype:Resistant | Treatment:Control	RAW_FILE_NAME=HILIC-RN25-pos.mzXML
SUBJECT_SAMPLE_FACTORS           	-	HILIC-RN25-neg	Developmental stage (DPI):day 7 | Genotype:Resistant | Treatment:Control	RAW_FILE_NAME=HILIC-RN25-neg.mzXML
SUBJECT_SAMPLE_FACTORS           	-	C18-RN26-pos	Developmental stage (DPI):day 7 | Genotype:Resistant | Treatment:Control	RAW_FILE_NAME=C18-RN26-pos.mzXML
SUBJECT_SAMPLE_FACTORS           	-	C18-RN26-neg	Developmental stage (DPI):day 7 | Genotype:Resistant | Treatment:Control	RAW_FILE_NAME=C18-RN26-neg.mzXML
SUBJECT_SAMPLE_FACTORS           	-	HILIC-RN26-pos	Developmental stage (DPI):day 7 | Genotype:Resistant | Treatment:Control	RAW_FILE_NAME=HILIC-RN26-pos.mzXML
SUBJECT_SAMPLE_FACTORS           	-	HILIC-RN26-neg	Developmental stage (DPI):day 7 | Genotype:Resistant | Treatment:Control	RAW_FILE_NAME=HILIC-RN26-neg.mzXML
SUBJECT_SAMPLE_FACTORS           	-	C18-RN27-pos	Developmental stage (DPI):day 7 | Genotype:Resistant | Treatment:Control	RAW_FILE_NAME=C18-RN27-pos.mzXML
SUBJECT_SAMPLE_FACTORS           	-	C18-RN27-neg	Developmental stage (DPI):day 7 | Genotype:Resistant | Treatment:Control	RAW_FILE_NAME=C18-RN27-neg.mzXML
SUBJECT_SAMPLE_FACTORS           	-	HILIC-RN27-pos	Developmental stage (DPI):day 7 | Genotype:Resistant | Treatment:Control	RAW_FILE_NAME=HILIC-RN27-pos.mzXML
SUBJECT_SAMPLE_FACTORS           	-	HILIC-RN27-neg	Developmental stage (DPI):day 7 | Genotype:Resistant | Treatment:Control	RAW_FILE_NAME=HILIC-RN27-neg.mzXML
SUBJECT_SAMPLE_FACTORS           	-	C18-RN28-pos	Developmental stage (DPI):day 7 | Genotype:Resistant | Treatment:Treated with Plasmodiophora brassicae	RAW_FILE_NAME=C18-RN28-pos.mzXML
SUBJECT_SAMPLE_FACTORS           	-	C18-RN28-neg	Developmental stage (DPI):day 7 | Genotype:Resistant | Treatment:Treated with Plasmodiophora brassicae	RAW_FILE_NAME=C18-RN28-neg.mzXML
SUBJECT_SAMPLE_FACTORS           	-	HILIC-RN28-pos	Developmental stage (DPI):day 7 | Genotype:Resistant | Treatment:Treated with Plasmodiophora brassicae	RAW_FILE_NAME=HILIC-RN28-pos.mzXML
SUBJECT_SAMPLE_FACTORS           	-	HILIC-RN28-neg	Developmental stage (DPI):day 7 | Genotype:Resistant | Treatment:Treated with Plasmodiophora brassicae	RAW_FILE_NAME=HILIC-RN28-neg.mzXML
SUBJECT_SAMPLE_FACTORS           	-	C18-RN29-pos	Developmental stage (DPI):day 7 | Genotype:Resistant | Treatment:Treated with Plasmodiophora brassicae	RAW_FILE_NAME=C18-RN29-pos.mzXML
SUBJECT_SAMPLE_FACTORS           	-	C18-RN29-neg	Developmental stage (DPI):day 7 | Genotype:Resistant | Treatment:Treated with Plasmodiophora brassicae	RAW_FILE_NAME=C18-RN29-neg.mzXML
SUBJECT_SAMPLE_FACTORS           	-	HILIC-RN29-pos	Developmental stage (DPI):day 7 | Genotype:Resistant | Treatment:Treated with Plasmodiophora brassicae	RAW_FILE_NAME=HILIC-RN29-pos.mzXML
SUBJECT_SAMPLE_FACTORS           	-	HILIC-RN29-neg	Developmental stage (DPI):day 7 | Genotype:Resistant | Treatment:Treated with Plasmodiophora brassicae	RAW_FILE_NAME=HILIC-RN29-neg.mzXML
SUBJECT_SAMPLE_FACTORS           	-	C18-RN30-pos	Developmental stage (DPI):day 7 | Genotype:Resistant | Treatment:Treated with Plasmodiophora brassicae	RAW_FILE_NAME=C18-RN30-pos.mzXML
SUBJECT_SAMPLE_FACTORS           	-	C18-RN30-neg	Developmental stage (DPI):day 7 | Genotype:Resistant | Treatment:Treated with Plasmodiophora brassicae	RAW_FILE_NAME=C18-RN30-neg.mzXML
SUBJECT_SAMPLE_FACTORS           	-	HILIC-RN30-pos	Developmental stage (DPI):day 7 | Genotype:Resistant | Treatment:Treated with Plasmodiophora brassicae	RAW_FILE_NAME=HILIC-RN30-pos.mzXML
SUBJECT_SAMPLE_FACTORS           	-	HILIC-RN30-neg	Developmental stage (DPI):day 7 | Genotype:Resistant | Treatment:Treated with Plasmodiophora brassicae	RAW_FILE_NAME=HILIC-RN30-neg.mzXML
SUBJECT_SAMPLE_FACTORS           	-	C18-RN31-pos	Developmental stage (DPI):day 7 | Genotype:Susceptible | Treatment:Control	RAW_FILE_NAME=C18-RN31-pos.mzXML
SUBJECT_SAMPLE_FACTORS           	-	C18-RN31-neg	Developmental stage (DPI):day 7 | Genotype:Susceptible | Treatment:Control	RAW_FILE_NAME=C18-RN31-neg.mzXML
SUBJECT_SAMPLE_FACTORS           	-	HILIC-RN31-pos	Developmental stage (DPI):day 7 | Genotype:Susceptible | Treatment:Control	RAW_FILE_NAME=HILIC-RN31-pos.mzXML
SUBJECT_SAMPLE_FACTORS           	-	HILIC-RN31-neg	Developmental stage (DPI):day 7 | Genotype:Susceptible | Treatment:Control	RAW_FILE_NAME=HILIC-RN31-neg.mzXML
SUBJECT_SAMPLE_FACTORS           	-	C18-RN32-pos	Developmental stage (DPI):day 7 | Genotype:Susceptible | Treatment:Control	RAW_FILE_NAME=C18-RN32-pos.mzXML
SUBJECT_SAMPLE_FACTORS           	-	C18-RN32-neg	Developmental stage (DPI):day 7 | Genotype:Susceptible | Treatment:Control	RAW_FILE_NAME=C18-RN32-neg.mzXML
SUBJECT_SAMPLE_FACTORS           	-	HILIC-RN32-pos	Developmental stage (DPI):day 7 | Genotype:Susceptible | Treatment:Control	RAW_FILE_NAME=HILIC-RN32-pos.mzXML
SUBJECT_SAMPLE_FACTORS           	-	HILIC-RN32-neg	Developmental stage (DPI):day 7 | Genotype:Susceptible | Treatment:Control	RAW_FILE_NAME=HILIC-RN32-neg.mzXML
SUBJECT_SAMPLE_FACTORS           	-	C18-RN33-pos	Developmental stage (DPI):day 7 | Genotype:Susceptible | Treatment:Control	RAW_FILE_NAME=C18-RN33-pos.mzXML
SUBJECT_SAMPLE_FACTORS           	-	C18-RN33-neg	Developmental stage (DPI):day 7 | Genotype:Susceptible | Treatment:Control	RAW_FILE_NAME=C18-RN33-neg.mzXML
SUBJECT_SAMPLE_FACTORS           	-	HILIC-RN33-pos	Developmental stage (DPI):day 7 | Genotype:Susceptible | Treatment:Control	RAW_FILE_NAME=HILIC-RN33-pos.mzXML
SUBJECT_SAMPLE_FACTORS           	-	HILIC-RN33-neg	Developmental stage (DPI):day 7 | Genotype:Susceptible | Treatment:Control	RAW_FILE_NAME=HILIC-RN33-neg.mzXML
SUBJECT_SAMPLE_FACTORS           	-	C18-RN34-pos	Developmental stage (DPI):day 7 | Genotype:Susceptible | Treatment:Treated with Plasmodiophora brassicae	RAW_FILE_NAME=C18-RN34-pos.mzXML
SUBJECT_SAMPLE_FACTORS           	-	C18-RN34-neg	Developmental stage (DPI):day 7 | Genotype:Susceptible | Treatment:Treated with Plasmodiophora brassicae	RAW_FILE_NAME=C18-RN34-neg.mzXML
SUBJECT_SAMPLE_FACTORS           	-	HILIC-RN34-pos	Developmental stage (DPI):day 7 | Genotype:Susceptible | Treatment:Treated with Plasmodiophora brassicae	RAW_FILE_NAME=HILIC-RN34-pos.mzXML
SUBJECT_SAMPLE_FACTORS           	-	HILIC-RN34-neg	Developmental stage (DPI):day 7 | Genotype:Susceptible | Treatment:Treated with Plasmodiophora brassicae	RAW_FILE_NAME=HILIC-RN34-neg.mzXML
SUBJECT_SAMPLE_FACTORS           	-	C18-RN35-pos	Developmental stage (DPI):day 7 | Genotype:Susceptible | Treatment:Treated with Plasmodiophora brassicae	RAW_FILE_NAME=C18-RN35-pos.mzXML
SUBJECT_SAMPLE_FACTORS           	-	C18-RN35-neg	Developmental stage (DPI):day 7 | Genotype:Susceptible | Treatment:Treated with Plasmodiophora brassicae	RAW_FILE_NAME=C18-RN35-neg.mzXML
SUBJECT_SAMPLE_FACTORS           	-	HILIC-RN35-pos	Developmental stage (DPI):day 7 | Genotype:Susceptible | Treatment:Treated with Plasmodiophora brassicae	RAW_FILE_NAME=HILIC-RN35-pos.mzXML
SUBJECT_SAMPLE_FACTORS           	-	HILIC-RN35-neg	Developmental stage (DPI):day 7 | Genotype:Susceptible | Treatment:Treated with Plasmodiophora brassicae	RAW_FILE_NAME=HILIC-RN35-neg.mzXML
SUBJECT_SAMPLE_FACTORS           	-	C18-RN36-pos	Developmental stage (DPI):day 7 | Genotype:Susceptible | Treatment:Treated with Plasmodiophora brassicae	RAW_FILE_NAME=C18-RN36-pos.mzXML
SUBJECT_SAMPLE_FACTORS           	-	C18-RN36-neg	Developmental stage (DPI):day 7 | Genotype:Susceptible | Treatment:Treated with Plasmodiophora brassicae	RAW_FILE_NAME=C18-RN36-neg.mzXML
SUBJECT_SAMPLE_FACTORS           	-	HILIC-RN36-pos	Developmental stage (DPI):day 7 | Genotype:Susceptible | Treatment:Treated with Plasmodiophora brassicae	RAW_FILE_NAME=HILIC-RN36-pos.mzXML
SUBJECT_SAMPLE_FACTORS           	-	HILIC-RN36-neg	Developmental stage (DPI):day 7 | Genotype:Susceptible | Treatment:Treated with Plasmodiophora brassicae	RAW_FILE_NAME=HILIC-RN36-neg.mzXML
#COLLECTION
CO:COLLECTION_SUMMARY            	A total of 36 samples comprised two types of genotypes [CR (5 individuals pooled
CO:COLLECTION_SUMMARY            	in each biological replicate) and CS (5 individuals pooled in each biological
CO:COLLECTION_SUMMARY            	replicate)], two treatments (inoculated and uninoculated) and three biological
CO:COLLECTION_SUMMARY            	replicates generated from three independent experiments and collected at 1-, 4-,
CO:COLLECTION_SUMMARY            	and 7–DPI were used. Immediately after collection, all root samples were
CO:COLLECTION_SUMMARY            	flash-frozen in liquid nitrogen and stored at -80 °C.
CO:COLLECTION_PROTOCOL_FILENAME  	Collection_protocol-canola
CO:SAMPLE_TYPE                   	Plant
CO:COLLECTION_LOCATION           	Alberta, Canada
CO:STORAGE_CONDITIONS            	-80℃
CO:COLLECTION_VIALS              	2 mL round-bottom Eppendorf tubes
CO:STORAGE_VIALS                 	2 mL round-bottom Eppendorf tubes
#TREATMENT
TR:TREATMENT_SUMMARY             	A single spore-derived isolate of P. brassicae pathotype 3H (P3H) was used in
TR:TREATMENT_SUMMARY             	this study. To assess the disease progression post-inoculation, histology, and
TR:TREATMENT_SUMMARY             	scanning electron microscopy (SEM) was conducted on P. brassicae inoculated and
TR:TREATMENT_SUMMARY             	uninoculated root tissues. Samples were collected by Adhikary as follows:
TR:TREATMENT_SUMMARY             	seedlings were randomly selected, gently uprooted from the soil, the roots were
TR:TREATMENT_SUMMARY             	washed thoroughly and collected in triplicates at after 24 hours of inoculation
TR:TREATMENT_SUMMARY             	[hereinafter indicated as 1-day post-inoculation (DPI)], 4-, and 7-DPI.
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	Approximately 100 mg of fresh weight of root tissue samples (n=3) were aliquoted
SP:SAMPLEPREP_SUMMARY            	from finely homogenized frozen root tissues, placed into 2 mL safe-lock
SP:SAMPLEPREP_SUMMARY            	centrifuge tubes with two zirconium oxide beads, flash frozen in liquid nitrogen
SP:SAMPLEPREP_SUMMARY            	and stored at − 80 °C until further processing. To accommodate for different
SP:SAMPLEPREP_SUMMARY            	extraction procedures, two sample sets were prepared from the same bulk to
SP:SAMPLEPREP_SUMMARY            	independently analyze the content of primary metabolites (free amino acids,
SP:SAMPLEPREP_SUMMARY            	sugars and sugar phosphates, organic acids), and secondary metabolites
SP:SAMPLEPREP_SUMMARY            	(glucosinolates) using High Performance Liquid Chromatography –
SP:SAMPLEPREP_SUMMARY            	High-Resolution Accurate Mass – Full Scan Mass Spectrometry (HPLC-(HRAM)-MS).
SP:SAMPLEPREP_SUMMARY            	Samples for extraction of primary and secondary metabolites were spiked with
SP:SAMPLEPREP_SUMMARY            	10ng of two labeled aromatic CKs (13C5-oT and 2H7-BAR) and extracted with
SP:SAMPLEPREP_SUMMARY            	ice-cold methanol (methanol-water [8:2, v/v]) following procedures of Chen et al
SP:SAMPLEPREP_SUMMARY            	3 with modifications. Each filtered extract was split in half and each 500 µL
SP:SAMPLEPREP_SUMMARY            	transferred to a new 2 mL tube. Divided extracts were evaporated to dryness at
SP:SAMPLEPREP_SUMMARY            	ambient temperature in a speed vac concentrator. Sample residues designed for
SP:SAMPLEPREP_SUMMARY            	the analysis of primary metabolites were redissolved in 500 µl of 90%
SP:SAMPLEPREP_SUMMARY            	acetonitrile (acetonitrile: water, v/v) and samples for the analysis of
SP:SAMPLEPREP_SUMMARY            	glucosinolates were redissolved in 500 µL of 5% acetonitrile with 0.1% formic
SP:SAMPLEPREP_SUMMARY            	acid (acetonitrile: formic acid: water, v/v/v). Additionally, sample mixtures
SP:SAMPLEPREP_SUMMARY            	composed of 10uL of each sample extract were prepared separately for primary and
SP:SAMPLEPREP_SUMMARY            	secondary metabolite analysis and used to generate MS/MS data for compound
SP:SAMPLEPREP_SUMMARY            	identification. All samples were filtered using 0.2 µm PVDF spin filter with 2
SP:SAMPLEPREP_SUMMARY            	mL receiver tubes (InnoSep Spin, Canadian Life Sciences, Peterborough, Canada)
SP:SAMPLEPREP_SUMMARY            	and transferred to insert-equipped 2 mL HPLC vials. A volume of 25 μL of each
SP:SAMPLEPREP_SUMMARY            	sample was injected into a Dionex UltiMate 3000 HPLC coupled to a QExactive
SP:SAMPLEPREP_SUMMARY            	Orbitrap mass spectrometer.
SP:SAMPLEPREP_PROTOCOL_FILENAME  	Protocol_methods-canola
SP:EXTRACTION_METHOD             	80% MeOH
SP:EXTRACT_CLEANUP               	0.2 µm PVDF spin filter with 2 mL receiver tubes
SP:EXTRACT_STORAGE               	-20℃
SP:SAMPLE_RESUSPENSION           	Primary metabolites - 90% ACN, glucosinolates - 5% ACN
SP:SAMPLE_SPIKING                	two labeled aromatic CKs (13C5-oT and 2H7-BAR)
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_SUMMARY        	Primary metabolites were resolved with an InfinityLab Poroshell 120 HILIC-Z
CH:CHROMATOGRAPHY_SUMMARY        	column (2.1 × 100 mm, 2.7 µm; Agilent, Santa Clara, CA, US), using a flow rate
CH:CHROMATOGRAPHY_SUMMARY        	of 0.2 mL min-1 with a mobile phase of 10 mM ammonium bicarbonate in water (A)
CH:CHROMATOGRAPHY_SUMMARY        	and 10 mM ammonium bicarbonate in 95% acetonitrile (B). The following gradient
CH:CHROMATOGRAPHY_SUMMARY        	was used to elute the analytes: mobile phase 100% B decreased to 90% over 2.5
CH:CHROMATOGRAPHY_SUMMARY        	min and to 50% over the next 5 min and returned to 100% over 0.5 min for 12 min
CH:CHROMATOGRAPHY_SUMMARY        	of column re-equilibration.
CH:CHROMATOGRAPHY_TYPE           	HILIC
CH:INSTRUMENT_NAME               	Thermo Dionex Ultimate 3000 RS
CH:COLUMN_NAME                   	Agilent, InfinityLab Poroshell 120 HILIC-Z (2.1 × 100 mm, 2.7 µm)
CH:FLOW_RATE                     	0.2 ml/min
CH:COLUMN_TEMPERATURE            	RT
CH:SOLVENT_A                     	10 mM ammonium bicarbonate in water
CH:SOLVENT_B                     	10 mM ammonium bicarbonate in 95% acetonitrile
CH:INJECTION_TEMPERATURE         	4
#ANALYSIS
AN:ANALYSIS_TYPE                 	MS
AN:LABORATORY_NAME               	WQC
AN:OPERATOR_NAME                 	Anna Kisiala
#MS
MS:INSTRUMENT_NAME               	Thermo Q Exactive Orbitrap
MS:INSTRUMENT_TYPE               	Orbitrap
MS:MS_TYPE                       	ESI
MS:ION_MODE                      	NEGATIVE
MS:MS_COMMENTS                   	The Orbitrap QExactive was operated with a heated electrospray ionization (HESI)
MS:MS_COMMENTS                   	probe in negative mode. Temperatures of the HESI-II auxiliary gas heater and
MS:MS_COMMENTS                   	capillary were 450 and 300 °C, respectively, and the spray voltage was 3.9 kV.
MS:MS_COMMENTS                   	Sheath, auxiliary, and sweep gases were operated at 30, 8, and 0 (arbitrary
MS:MS_COMMENTS                   	units), respectively, and the S-lens RF level was 60. Each sample was analyzed
MS:MS_COMMENTS                   	using a mass range of m/z 70−900, and data were acquired at 70,000 resolution,
MS:MS_COMMENTS                   	automatic gain control (AGC) target of 1e6, and maximum injection time (IT) of
MS:MS_COMMENTS                   	100 ms. Additionally, the top 10 data-dependent acquisition experiments were
MS:MS_COMMENTS                   	performed for the mixed sample from each group (HILIC and C18) to obtain
MS:MS_COMMENTS                   	compound MS/MS spectra. Processing of all full scan and ddMS2 data was conducted
MS:MS_COMMENTS                   	using the Xcalibur 4.1 software. Metabolites were identified by accurate mass,
MS:MS_COMMENTS                   	comparison of retention times to authentic standards or by accurate mass and
MS:MS_COMMENTS                   	comparison of fragmentation patterns to MS/MS databases (METLIN, PubChem).
MS:MS_COMMENTS                   	Metabolite relative concentration was normalized based on the average recoveries
MS:MS_COMMENTS                   	of 13C5-oT and 2H7-BAR as the internal standards
#MS_METABOLITE_DATA
MS_METABOLITE_DATA:UNITS	normalized relative level
MS_METABOLITE_DATA_START
Samples	HILIC-RN1-neg	HILIC-RN2-neg	HILIC-RN3-neg	HILIC-RN4-neg	HILIC-RN5-neg	HILIC-RN6-neg	HILIC-RN7-neg	HILIC-RN8-neg	HILIC-RN9-neg	HILIC-RN10-neg	HILIC-RN11-neg	HILIC-RN12-neg	HILIC-RN13-neg	HILIC-RN14-neg	HILIC-RN15-neg	HILIC-RN16-neg	HILIC-RN17-neg	HILIC-RN18-neg	HILIC-RN19-neg	HILIC-RN20-neg	HILIC-RN21-neg	HILIC-RN22-neg	HILIC-RN23-neg	HILIC-RN24-neg	HILIC-RN25-neg	HILIC-RN26-neg	HILIC-RN27-neg	HILIC-RN28-neg	HILIC-RN29-neg	HILIC-RN30-neg	HILIC-RN31-neg	HILIC-RN32-neg	HILIC-RN33-neg	HILIC-RN34-neg	HILIC-RN35-neg	HILIC-RN36-neg
Factors	Developmental stage (DPI):day 1 | Genotype:Resistant | Treatment:Control	Developmental stage (DPI):day 1 | Genotype:Resistant | Treatment:Control	Developmental stage (DPI):day 1 | Genotype:Resistant | Treatment:Control	Developmental stage (DPI):day 1 | Genotype:Resistant | Treatment:Treated with Plasmodiophora brassicae	Developmental stage (DPI):day 1 | Genotype:Resistant | Treatment:Treated with Plasmodiophora brassicae	Developmental stage (DPI):day 1 | Genotype:Resistant | Treatment:Treated with Plasmodiophora brassicae	Developmental stage (DPI):day 1 | Genotype:Susceptible | Treatment:Control	Developmental stage (DPI):day 1 | Genotype:Susceptible | Treatment:Control	Developmental stage (DPI):day 1 | Genotype:Susceptible | Treatment:Control	Developmental stage (DPI):day 1 | Genotype:Susceptible | Treatment:Treated with Plasmodiophora brassicae	Developmental stage (DPI):day 1 | Genotype:Susceptible | Treatment:Treated with Plasmodiophora brassicae	Developmental stage (DPI):day 1 | Genotype:Susceptible | Treatment:Treated with Plasmodiophora brassicae	Developmental stage (DPI):day 4 | Genotype:Resistant | Treatment:Control	Developmental stage (DPI):day 4 | Genotype:Resistant | Treatment:Control	Developmental stage (DPI):day 4 | Genotype:Resistant | Treatment:Control	Developmental stage (DPI):day 4 | Genotype:Resistant | Treatment:Treated with Plasmodiophora brassicae	Developmental stage (DPI):day 4 | Genotype:Resistant | Treatment:Treated with Plasmodiophora brassicae	Developmental stage (DPI):day 4 | Genotype:Resistant | Treatment:Treated with Plasmodiophora brassicae	Developmental stage (DPI):day 4 | Genotype:Susceptible | Treatment:Control	Developmental stage (DPI):day 4 | Genotype:Susceptible | Treatment:Control	Developmental stage (DPI):day 4 | Genotype:Susceptible | Treatment:Control	Developmental stage (DPI):day 4 | Genotype:Susceptible | Treatment:Treated with Plasmodiophora brassicae	Developmental stage (DPI):day 4 | Genotype:Susceptible | Treatment:Treated with Plasmodiophora brassicae	Developmental stage (DPI):day 4 | Genotype:Susceptible | Treatment:Treated with Plasmodiophora brassicae	Developmental stage (DPI):day 7 | Genotype:Resistant | Treatment:Control	Developmental stage (DPI):day 7 | Genotype:Resistant | Treatment:Control	Developmental stage (DPI):day 7 | Genotype:Resistant | Treatment:Control	Developmental stage (DPI):day 7 | Genotype:Resistant | Treatment:Treated with Plasmodiophora brassicae	Developmental stage (DPI):day 7 | Genotype:Resistant | Treatment:Treated with Plasmodiophora brassicae	Developmental stage (DPI):day 7 | Genotype:Resistant | Treatment:Treated with Plasmodiophora brassicae	Developmental stage (DPI):day 7 | Genotype:Susceptible | Treatment:Control	Developmental stage (DPI):day 7 | Genotype:Susceptible | Treatment:Control	Developmental stage (DPI):day 7 | Genotype:Susceptible | Treatment:Control	Developmental stage (DPI):day 7 | Genotype:Susceptible | Treatment:Treated with Plasmodiophora brassicae	Developmental stage (DPI):day 7 | Genotype:Susceptible | Treatment:Treated with Plasmodiophora brassicae	Developmental stage (DPI):day 7 | Genotype:Susceptible | Treatment:Treated with Plasmodiophora brassicae
Fructose	1.121	0.854	0.424	0.419	0.917	0.627	1.270	1.727	0.930	1.380	0.668	1.054	0.903	0.666	0.839	0.790	0.872	0.443	1.144	0.992	1.203	1.564	1.127	1.130	2.353	1.857	1.736	2.670	4.024	1.365	2.459	0.750	0.948	1.302	1.590	0.423
Glucose	1.589	0.489	1.150	1.085	2.161	1.080	3.782	2.911	1.136	2.075	0.655	1.346	1.348	0.756	1.537	0.860	1.046	0.660	1.132	1.042	1.905	0.910	0.780	1.194	1.577	1.908	2.203	2.261	3.741	2.695	0.830	0.618	1.255	0.884	0.591	0.771
Mannitol	0.533	0.159	0.062	0.038	2.709	1.430	0.103	0.110	0.028	0.065	0.019	0.037	0.054	0.022	0.049	0.026	0.609	0.023	1.643	1.763	2.445	0.031	0.034	0.035	0.066	1.502	0.097	1.927	0.131	1.308	0.040	0.052	0.042	0.027	0.025	0.029
Monosaccharide-6-phosphate	1.815	0.961	0.447	0.354	0.534	0.543	2.170	0.858	0.652	1.310	0.236	0.415	0.754	0.813	0.759	0.509	0.463	0.296	1.450	0.450	0.383	1.729	0.322	0.327	0.536	0.715	0.396	2.401	1.128	1.133	1.193	0.779	0.302	1.639	0.293	0.124
Ribose	1.063	2.007	1.454	0.926	1.035	0.813	1.667		0.968	1.516	0.471	0.684	0.584	0.866	0.859	0.547	1.167	0.771	1.042	0.489	1.105	0.503	0.349	0.207	1.033	0.504	2.058	2.261		0.178	1.865		0.794	0.324	0.106	1.326
Ribose-5-phosphate	1.287	0.437	0.301	0.760	1.272	0.873	0.899	1.093	0.402	0.659	0.455	0.558	0.994	0.585	1.318	0.803	0.789	0.601	0.355	0.465	0.698	0.583	0.387	0.419	1.237	1.805	1.918	1.231	3.589	1.287	0.726	0.842	0.465	0.928	1.948	0.214
Sedoheptulose-7-phosphate	1.505	0.716	0.909	0.991	2.121	1.898	1.971	1.639	1.130	1.716	0.677	1.692	0.845	0.499	1.657	0.785	0.953	0.966	0.777	0.794	2.170	0.369	0.557	1.470	0.946	1.793	3.291	0.574	3.475	3.291	0.653	0.773	1.549	0.894	0.614	0.473
Sucrose	1.356	0.785	0.592	0.484	1.293	0.733	1.963	1.521	0.654	1.037	0.514	0.797	0.827	0.615	1.436	0.672	0.768	0.577	0.931	0.718	1.556	1.435	0.541	0.900	1.062	1.337	2.440	1.936	3.115	4.181	1.697	0.585	1.947	0.593	0.585	1.128
2-hydroxyglutaric acid	0.191	0.218	0.772	0.541	1.323	0.695	1.160	0.685	0.406	0.843	0.302	0.441	0.747	0.512	1.064	0.518	0.570	0.310	0.415	0.693	0.526	0.517	0.442	0.298	2.353	0.699	0.831	1.764	0.958	1.092	1.274	0.289	0.389	0.469	0.184	0.295
Alpha-Ketoglutaric acid	1.374	0.741	1.591	1.578	3.341	1.293	0.393	1.242	0.967	1.441	0.856	0.863	0.626	0.372	1.965	0.469	0.543	0.395	0.341	0.317	0.508	1.952	0.204	0.366	0.095	1.844	2.075	1.752	2.299	1.040	0.420	0.596	0.432	0.428	0.113	0.274
Cis-Aconitic acid	1.691	0.799	0.860	0.660	1.275	1.262	1.080	1.118	0.762	0.954	0.443	0.580	0.686	0.877	0.932	0.880	0.877	0.424	1.047	0.992	0.784	0.678	0.693	0.671	2.353	0.959	1.762	1.732	2.341	1.832	0.822	0.434	0.539	0.640	0.294	0.075
Citric acid	2.476	1.821	0.882	0.587	0.822	1.122	1.566	0.968	0.970	1.195	0.351	0.488	0.775	1.000	1.572	0.564	0.518	0.319	0.843	0.352	0.234	1.681	0.182	0.221	0.267	0.369	0.762	1.492	0.796	1.376	1.231	0.412	0.218	0.750	0.377	0.517
Fumaric acid	2.119	1.216	1.177	1.041	2.182	2.148	3.306	2.197	1.428	1.897	0.657	1.565	0.968	0.691	2.136	0.759	0.903	0.880	1.041	0.767	1.903	0.656	0.542	1.199	0.902	1.718	2.934	0.779	3.190	3.583	1.162	0.948	1.430	0.879	0.527	0.759
Gluconic acid	0.636	0.136	1.022	0.489	1.147	0.593	0.941	0.675	0.505	0.905	0.333	0.603	1.184	0.660	1.006	0.484	0.495	0.371	0.506	0.446	0.617	0.430	0.343	0.333	1.324	1.545	1.319	2.798	2.226	1.706	0.670	0.558	0.484	0.665	0.774	0.150
Lactic acid	0.662	0.740	0.492	0.228	0.515	0.533	1.787	0.337	0.280	0.389	0.143	0.216	0.673	0.545	0.527	0.283	0.547	0.230	0.408	0.402	0.217	2.089	0.675	0.055	1.211	1.746	0.868	2.272	1.451	0.195	1.092	0.413	0.039	0.740	0.782	0.298
Malic acid	2.067	1.212	1.147	1.055	2.210	2.179	3.298	2.431	1.428	1.888	0.657	1.618	0.934	0.664	2.127	0.739	0.900	0.885	1.049	0.741	1.934	0.623	0.516	1.213	0.824	1.672	2.906	0.690	3.058	3.485	1.052	0.903	1.412	0.855	0.404	0.605
Pyruvic acid	1.109	0.115	0.881	1.381	3.247	1.635	2.030	3.426	1.135	1.825	0.985	1.719	1.368	0.956	1.405	1.272	1.445	0.900	1.135	1.424	2.661	0.462	0.805	1.215	2.070	2.587	3.409	1.506	4.977	1.630	0.593	0.564	1.443	0.864	0.162	0.413
Succinic acid	1.802	1.315	1.322	1.068	2.361	1.063	1.610	1.930	0.724	2.075	0.723	0.753	0.659	0.537	1.001	0.513	0.633	0.354	0.670	0.454	0.762	0.427	0.611	0.468	2.121	1.486	0.881	1.721	1.687	1.060	0.868	0.497	0.376	0.647	0.384	0.267
MS_METABOLITE_DATA_END
#METABOLITES
METABOLITES_START
metabolite_name	quanitfied m/z	PubChem ID	KEGG ID
Fructose	179.05611	5984	C02336
Glucose	179.05611	5793	C00031
Mannitol	181.07176	6251	D00062
Monosaccharide-6-phosphate
Ribose	149.04555	5779	C00121
Ribose-5-phosphate	229.01188	77982	C00117
Sedoheptulose-7-phosphate	289.03301	165007	C05382
Sucrose	341.10893	5988	C00089
2-hydroxyglutaric acid	147.0299	43	C02630
Alpha-Ketoglutaric acid	145.01425	51	C00026
Cis-Aconitic acid	173.00916	309	C00417
Citric acid	191.01973	311	D00037
Fumaric acid	115.00368	444972	C00122  
Gluconic acid	195.05103	10690	C00257
Lactic acid	89.02442	612	C00186
Malic acid	133.01425	525	C00149
Pyruvic acid	87.00877	1060	C00022
Succinic acid	117.01933	1110	C00042
METABOLITES_END
#END