#METABOLOMICS WORKBENCH yli_20221021_110526 DATATRACK_ID:3531 STUDY_ID:ST002336 ANALYSIS_ID:AN003818 VERSION 1 CREATED_ON 09-06-2023 #PROJECT PR:PROJECT_TITLE Characterizing the intrauterine environment via untargeted metabolomics PR:PROJECT_TITLE profiling of maternal blood collected during pregnancy (Project Viva) PR:PROJECT_TYPE C18 Reversed-Phase Broad Spectrum Metabolomics PR:PROJECT_SUMMARY This project is funded by the NIH Environmental influences on Child Health PR:PROJECT_SUMMARY Outcomes (ECHO) program, and uses data from five cohorts: Healthy Start, Project PR:PROJECT_SUMMARY Viva, Atlanta ECHO, Paneth Cohort, and PETALS. This project will generate new PR:PROJECT_SUMMARY untargeted metabolomics profiling data at two time-points in pregnancy for all PR:PROJECT_SUMMARY women in order to identify trimester-specific metabolomics signatures and PR:PROJECT_SUMMARY longitudinal metabolomics trajectories associated with offspring birth size; PR:PROJECT_SUMMARY identify the contribution of maternal sociodemographic and perinatal PR:PROJECT_SUMMARY characteristics to the metabolomics signatures. PR:INSTITUTE NC HHEAR Hub PR:DEPARTMENT Untargeted Analysis PR:LABORATORY Sumner Lab PR:LAST_NAME Li PR:FIRST_NAME Yuanyuan PR:ADDRESS 500 Laureate Way, Kannapolis, NC 28081 PR:EMAIL yuanyli4@unc.edu PR:PHONE 9843770693 PR:FUNDING_SOURCE This research was supported by the Environmental influences on Child Health PR:FUNDING_SOURCE Outcomes (ECHO) program, Office of The Director, National Institutes of Health. PR:FUNDING_SOURCE The content is solely the responsibility of the authors and does not necessarily PR:FUNDING_SOURCE represent the official views of the National Institutes of Health. Project Viva PR:FUNDING_SOURCE is an ECHO cohort which is supported by the following ECHO Program PR:FUNDING_SOURCE Collaborators: ECHO Coordinating Center: Duke Clinical Research Institute, PR:FUNDING_SOURCE Durham, North Carolina: Smith PB, Newby KL, Benjamin DK; U2C OD023375; ECHO Data PR:FUNDING_SOURCE Analysis Center: Johns Hopkins University Bloomberg School of Public Health, PR:FUNDING_SOURCE Baltimore, Maryland: Jacobson LP; Research Triangle Institute, Durham, North PR:FUNDING_SOURCE Carolina: Catellier D; U24 OD023382; North Carolina Human Health Exposure PR:FUNDING_SOURCE Analysis Resource Hub: Research Triangle Institute: Fennell T, University of PR:FUNDING_SOURCE North Carolina at Chapel Hill: Sumner S, University of North Carolina at PR:FUNDING_SOURCE Charlotte: Du X; U2C ES030857; Human Health Exposure Analysis Resource PR:FUNDING_SOURCE Coordinating Center: Westat, Inc., Rockville, Maryland: O’Brien B; U24 PR:FUNDING_SOURCE ES026539 PR:DOI http://dx.doi.org/10.21228/M8TT5H #STUDY ST:STUDY_TITLE Characterizing the intrauterine environment via untargeted metabolomics ST:STUDY_TITLE profiling of maternal blood collected during pregnancy (Project Viva) ST:STUDY_TYPE Prospective cohort study ST:STUDY_SUMMARY Project Viva: Pregnant women were enrolled in Project Viva between 1999 and 2002 ST:STUDY_SUMMARY at their first prenatal visit at one of 8 obstetric clinics of Atrius Harvard ST:STUDY_SUMMARY Vanguard Medical Associates, a multispecialty group practice in eastern ST:STUDY_SUMMARY Massachusetts. Eligible mothers were fluent in English, had singleton ST:STUDY_SUMMARY gestations, were <22 weeks gestation, and had no plans to move away from the ST:STUDY_SUMMARY study area. Research staff performed in-person study visits with participating ST:STUDY_SUMMARY mothers in the first (median gestational age 9.9 weeks) and second (median ST:STUDY_SUMMARY gestational age 28.1 weeks) trimesters of pregnancy, and with mothers and ST:STUDY_SUMMARY children during the first few days after delivery, during infancy (median age ST:STUDY_SUMMARY 6.3 months), in early childhood (median age 3.3 years), mid-childhood (median ST:STUDY_SUMMARY age 7.7 years), and adolescence (median age13 years). In this analysis, we will ST:STUDY_SUMMARY use data from 188 mother-child pairs in Project Viva with available information ST:STUDY_SUMMARY on prenatal PFAS concentrations, available umbilical cord serum samples at ST:STUDY_SUMMARY delivery, and outcomes of interest. Please contact MollyAn Killingbeck at ST:STUDY_SUMMARY mollyan.killingbeck@point32health.org for questions related to the subject ST:STUDY_SUMMARY characteristics and outcomes. This research was supported by the Environmental ST:STUDY_SUMMARY influences on Child Health Outcomes (ECHO) program, Office of The Director, ST:STUDY_SUMMARY National Institutes of Health. The content is solely the responsibility of the ST:STUDY_SUMMARY authors and does not necessarily represent the official views of the National ST:STUDY_SUMMARY Institutes of Health. Project Viva is an ECHO cohort which is supported by the ST:STUDY_SUMMARY following ECHO Program Collaborators: ECHO Coordinating Center: Duke Clinical ST:STUDY_SUMMARY Research Institute, Durham, North Carolina: Smith PB, Newby KL, Benjamin DK; U2C ST:STUDY_SUMMARY OD023375 ECHO Data Analysis Center: Johns Hopkins University Bloomberg School of ST:STUDY_SUMMARY Public Health, Baltimore, Maryland: Jacobson LP; Research Triangle Institute, ST:STUDY_SUMMARY Durham, North Carolina: Catellier D; U24 OD023382 North Carolina Human Health ST:STUDY_SUMMARY Exposure Analysis Resource Hub: Research Triangle Institute: Fennell T, ST:STUDY_SUMMARY University of North Carolina at Chapel Hill: Sumner S, University of North ST:STUDY_SUMMARY Carolina at Charlotte: Du X; U2C ES030857 Human Health Exposure Analysis ST:STUDY_SUMMARY Resource Coordinating Center: Westat, Inc., Rockville, Maryland: O’Brien B; ST:STUDY_SUMMARY U24 ES026539 ST:INSTITUTE Harvard Pilgrim Health Care Institute ST:DEPARTMENT Population Medicine ST:LABORATORY Channing ST:LAST_NAME Oken ST:FIRST_NAME Emily ST:ADDRESS 401 Park Drive, Ste 401, Boston, MA 02215 ST:EMAIL emily_oken@harvardpilgrim.org ST:PHONE 617-867-4835 ST:SUBMIT_DATE 2022-10-21 #SUBJECT SU:SUBJECT_TYPE Human SU:SUBJECT_SPECIES Homo sapiens SU:TAXONOMY_ID 9606 SU:GENDER Male and female #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS - PZOk_HHEAR_1 Sample Type:HHEAR Pool HHEAR-ECHO ID=N/A; 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RAW_FILE_NAME=PZOk_NIST_10 SUBJECT_SAMPLE_FACTORS - PZOk_NIST_11 Sample Type:NIST Pool HHEAR-ECHO ID=N/A; RAW_FILE_NAME=PZOk_NIST_11 SUBJECT_SAMPLE_FACTORS - PZOk_NIST_12 Sample Type:NIST Pool HHEAR-ECHO ID=N/A; RAW_FILE_NAME=PZOk_NIST_12 SUBJECT_SAMPLE_FACTORS - PZOk_NIST_13 Sample Type:NIST Pool HHEAR-ECHO ID=N/A; RAW_FILE_NAME=PZOk_NIST_13 SUBJECT_SAMPLE_FACTORS - PZOk_NIST_14 Sample Type:NIST Pool HHEAR-ECHO ID=N/A; RAW_FILE_NAME=PZOk_NIST_14 SUBJECT_SAMPLE_FACTORS - PZOk_NIST_15 Sample Type:NIST Pool HHEAR-ECHO ID=N/A; RAW_FILE_NAME=PZOk_NIST_15 SUBJECT_SAMPLE_FACTORS - PZOk_NIST_16 Sample Type:NIST Pool HHEAR-ECHO ID=N/A; RAW_FILE_NAME=PZOk_NIST_16 SUBJECT_SAMPLE_FACTORS - PZOk_NIST_17 Sample Type:NIST Pool HHEAR-ECHO ID=N/A; RAW_FILE_NAME=PZOk_NIST_17 SUBJECT_SAMPLE_FACTORS - PZOk_NIST_18 Sample Type:NIST Pool HHEAR-ECHO ID=N/A; RAW_FILE_NAME=PZOk_NIST_18 SUBJECT_SAMPLE_FACTORS - PZOk_NIST_19 Sample Type:NIST Pool HHEAR-ECHO ID=N/A; RAW_FILE_NAME=PZOk_NIST_19 SUBJECT_SAMPLE_FACTORS - PZOk_NIST_2 Sample Type:NIST Pool HHEAR-ECHO ID=N/A; RAW_FILE_NAME=PZOk_NIST_2 SUBJECT_SAMPLE_FACTORS - PZOk_NIST_20 Sample Type:NIST Pool HHEAR-ECHO ID=N/A; RAW_FILE_NAME=PZOk_NIST_20 SUBJECT_SAMPLE_FACTORS - PZOk_NIST_21 Sample Type:NIST Pool HHEAR-ECHO ID=N/A; RAW_FILE_NAME=PZOk_NIST_21 SUBJECT_SAMPLE_FACTORS - PZOk_NIST_22 Sample Type:NIST Pool HHEAR-ECHO ID=N/A; RAW_FILE_NAME=PZOk_NIST_22 SUBJECT_SAMPLE_FACTORS - PZOk_NIST_23 Sample Type:NIST Pool HHEAR-ECHO ID=N/A; RAW_FILE_NAME=PZOk_NIST_23 SUBJECT_SAMPLE_FACTORS - PZOk_NIST_24 Sample Type:NIST Pool HHEAR-ECHO ID=N/A; RAW_FILE_NAME=PZOk_NIST_24 SUBJECT_SAMPLE_FACTORS - PZOk_NIST_25 Sample Type:NIST Pool HHEAR-ECHO ID=N/A; RAW_FILE_NAME=PZOk_NIST_25 SUBJECT_SAMPLE_FACTORS - PZOk_NIST_26 Sample Type:NIST Pool HHEAR-ECHO ID=N/A; RAW_FILE_NAME=PZOk_NIST_26 SUBJECT_SAMPLE_FACTORS - PZOk_NIST_27 Sample Type:NIST Pool HHEAR-ECHO ID=N/A; RAW_FILE_NAME=PZOk_NIST_27 SUBJECT_SAMPLE_FACTORS - PZOk_NIST_28 Sample Type:NIST Pool HHEAR-ECHO ID=N/A; RAW_FILE_NAME=PZOk_NIST_28 SUBJECT_SAMPLE_FACTORS - PZOk_NIST_29 Sample Type:NIST Pool HHEAR-ECHO ID=N/A; RAW_FILE_NAME=PZOk_NIST_29 SUBJECT_SAMPLE_FACTORS - PZOk_NIST_3 Sample Type:NIST Pool HHEAR-ECHO ID=N/A; RAW_FILE_NAME=PZOk_NIST_3 SUBJECT_SAMPLE_FACTORS - PZOk_NIST_30 Sample Type:NIST Pool HHEAR-ECHO ID=N/A; RAW_FILE_NAME=PZOk_NIST_30 SUBJECT_SAMPLE_FACTORS - PZOk_NIST_31 Sample Type:NIST Pool HHEAR-ECHO ID=N/A; RAW_FILE_NAME=PZOk_NIST_31 SUBJECT_SAMPLE_FACTORS - PZOk_NIST_32 Sample Type:NIST Pool HHEAR-ECHO ID=N/A; RAW_FILE_NAME=PZOk_NIST_32 SUBJECT_SAMPLE_FACTORS - PZOk_NIST_33 Sample Type:NIST Pool HHEAR-ECHO ID=N/A; RAW_FILE_NAME=PZOk_NIST_33 SUBJECT_SAMPLE_FACTORS - PZOk_NIST_34 Sample Type:NIST Pool HHEAR-ECHO ID=N/A; RAW_FILE_NAME=PZOk_NIST_34 SUBJECT_SAMPLE_FACTORS - PZOk_NIST_35 Sample Type:NIST Pool HHEAR-ECHO ID=N/A; RAW_FILE_NAME=PZOk_NIST_35 SUBJECT_SAMPLE_FACTORS - PZOk_NIST_36 Sample Type:NIST Pool HHEAR-ECHO ID=N/A; RAW_FILE_NAME=PZOk_NIST_36 SUBJECT_SAMPLE_FACTORS - PZOk_NIST_37 Sample Type:NIST Pool HHEAR-ECHO ID=N/A; RAW_FILE_NAME=PZOk_NIST_37 SUBJECT_SAMPLE_FACTORS - PZOk_NIST_38 Sample Type:NIST Pool HHEAR-ECHO ID=N/A; RAW_FILE_NAME=PZOk_NIST_38 SUBJECT_SAMPLE_FACTORS - PZOk_NIST_39 Sample Type:NIST Pool HHEAR-ECHO ID=N/A; RAW_FILE_NAME=PZOk_NIST_39 SUBJECT_SAMPLE_FACTORS - PZOk_NIST_4 Sample Type:NIST Pool HHEAR-ECHO ID=N/A; RAW_FILE_NAME=PZOk_NIST_4 SUBJECT_SAMPLE_FACTORS - PZOk_NIST_40 Sample Type:NIST Pool HHEAR-ECHO ID=N/A; RAW_FILE_NAME=PZOk_NIST_40 SUBJECT_SAMPLE_FACTORS - PZOk_NIST_41 Sample Type:NIST Pool HHEAR-ECHO ID=N/A; RAW_FILE_NAME=PZOk_NIST_41 SUBJECT_SAMPLE_FACTORS - PZOk_NIST_42 Sample Type:NIST Pool HHEAR-ECHO ID=N/A; RAW_FILE_NAME=PZOk_NIST_42 SUBJECT_SAMPLE_FACTORS - PZOk_NIST_43 Sample Type:NIST Pool HHEAR-ECHO ID=N/A; RAW_FILE_NAME=PZOk_NIST_43 SUBJECT_SAMPLE_FACTORS - PZOk_NIST_44 Sample Type:NIST Pool HHEAR-ECHO ID=N/A; RAW_FILE_NAME=PZOk_NIST_44 SUBJECT_SAMPLE_FACTORS - PZOk_NIST_45 Sample Type:NIST Pool HHEAR-ECHO ID=N/A; RAW_FILE_NAME=PZOk_NIST_45 SUBJECT_SAMPLE_FACTORS - PZOk_NIST_5 Sample Type:NIST Pool HHEAR-ECHO ID=N/A; RAW_FILE_NAME=PZOk_NIST_5 SUBJECT_SAMPLE_FACTORS - PZOk_NIST_6 Sample Type:NIST Pool HHEAR-ECHO ID=N/A; RAW_FILE_NAME=PZOk_NIST_6 SUBJECT_SAMPLE_FACTORS - PZOk_NIST_7 Sample Type:NIST Pool HHEAR-ECHO ID=N/A; RAW_FILE_NAME=PZOk_NIST_7 SUBJECT_SAMPLE_FACTORS - PZOk_NIST_8 Sample Type:NIST Pool HHEAR-ECHO ID=N/A; RAW_FILE_NAME=PZOk_NIST_8 SUBJECT_SAMPLE_FACTORS - PZOk_NIST_9 Sample Type:NIST Pool HHEAR-ECHO ID=N/A; RAW_FILE_NAME=PZOk_NIST_9 SUBJECT_SAMPLE_FACTORS - PZOk_SP_1 Sample Type:Study Pool HHEAR-ECHO ID=EM-E0RV2-QCSP-001-12701; RAW_FILE_NAME=PZOk_SP_1 SUBJECT_SAMPLE_FACTORS - PZOk_SP_10 Sample Type:Study Pool HHEAR-ECHO ID=EM-4Q889-QCSP-010-12701; RAW_FILE_NAME=PZOk_SP_10 SUBJECT_SAMPLE_FACTORS - PZOk_SP_100 Sample Type:Study Pool HHEAR-ECHO ID=EM-CYRW2-QCSP-100-12701; RAW_FILE_NAME=PZOk_SP_100 SUBJECT_SAMPLE_FACTORS - PZOk_SP_101 Sample Type:Study Pool HHEAR-ECHO ID=EM-WWR85-QCSP-101-12701; RAW_FILE_NAME=PZOk_SP_101 SUBJECT_SAMPLE_FACTORS - PZOk_SP_102 Sample Type:Study Pool HHEAR-ECHO ID=EM-JX592-QCSP-102-12701; RAW_FILE_NAME=PZOk_SP_102 SUBJECT_SAMPLE_FACTORS - PZOk_SP_103 Sample Type:Study Pool HHEAR-ECHO ID=EM-9M9Q7-QCSP-103-12701; RAW_FILE_NAME=PZOk_SP_103 SUBJECT_SAMPLE_FACTORS - PZOk_SP_104 Sample Type:Study Pool HHEAR-ECHO ID=EM-KJ4J5-QCSP-104-12701; RAW_FILE_NAME=PZOk_SP_104 SUBJECT_SAMPLE_FACTORS - PZOk_SP_105 Sample Type:Study Pool HHEAR-ECHO ID=EM-KD5P4-QCSP-105-12701; RAW_FILE_NAME=PZOk_SP_105 SUBJECT_SAMPLE_FACTORS - PZOk_SP_106 Sample Type:Study Pool HHEAR-ECHO ID=EM-EK6T5-QCSP-106-12701; RAW_FILE_NAME=PZOk_SP_106 SUBJECT_SAMPLE_FACTORS - PZOk_SP_107 Sample Type:Study Pool HHEAR-ECHO ID=EM-YKUL2-QCSP-107-12701; RAW_FILE_NAME=PZOk_SP_107 SUBJECT_SAMPLE_FACTORS - PZOk_SP_108 Sample Type:Study Pool HHEAR-ECHO ID=EM-SNW67-QCSP-108-12701; RAW_FILE_NAME=PZOk_SP_108 SUBJECT_SAMPLE_FACTORS - PZOk_SP_109 Sample Type:Study Pool HHEAR-ECHO ID=EM-E77B8-QCSP-109-12701; RAW_FILE_NAME=PZOk_SP_109 SUBJECT_SAMPLE_FACTORS - PZOk_SP_11 Sample Type:Study Pool HHEAR-ECHO ID=EM-F3028-QCSP-011-12701; RAW_FILE_NAME=PZOk_SP_11 SUBJECT_SAMPLE_FACTORS - PZOk_SP_110 Sample Type:Study Pool HHEAR-ECHO ID=EM-UJ2V1-QCSP-110-12701; RAW_FILE_NAME=PZOk_SP_110 SUBJECT_SAMPLE_FACTORS - PZOk_SP_111 Sample Type:Study Pool HHEAR-ECHO ID=EM-GKYG7-QCSP-111-12701; RAW_FILE_NAME=PZOk_SP_111 SUBJECT_SAMPLE_FACTORS - PZOk_SP_112 Sample Type:Study Pool HHEAR-ECHO ID=EM-SW562-QCSP-112-12701; RAW_FILE_NAME=PZOk_SP_112 SUBJECT_SAMPLE_FACTORS - PZOk_SP_113 Sample Type:Study Pool HHEAR-ECHO ID=EM-SYV07-QCSP-113-12701; RAW_FILE_NAME=PZOk_SP_113 SUBJECT_SAMPLE_FACTORS - PZOk_SP_114 Sample Type:Study Pool HHEAR-ECHO ID=EM-G62H0-QCSP-114-12701; RAW_FILE_NAME=PZOk_SP_114 SUBJECT_SAMPLE_FACTORS - PZOk_SP_115 Sample Type:Study Pool HHEAR-ECHO ID=EM-V4DJ7-QCSP-115-12701; RAW_FILE_NAME=PZOk_SP_115 SUBJECT_SAMPLE_FACTORS - PZOk_SP_116 Sample Type:Study Pool HHEAR-ECHO ID=EM-H1SH7-QCSP-116-12701; RAW_FILE_NAME=PZOk_SP_116 SUBJECT_SAMPLE_FACTORS - PZOk_SP_117 Sample Type:Study Pool HHEAR-ECHO ID=EM-SJUM8-QCSP-117-12701; RAW_FILE_NAME=PZOk_SP_117 SUBJECT_SAMPLE_FACTORS - PZOk_SP_118 Sample Type:Study Pool HHEAR-ECHO ID=EM-WEDJ8-QCSP-118-12701; RAW_FILE_NAME=PZOk_SP_118 SUBJECT_SAMPLE_FACTORS - PZOk_SP_119 Sample Type:Study Pool HHEAR-ECHO ID=EM-U4WM3-QCSP-119-12701; RAW_FILE_NAME=PZOk_SP_119 SUBJECT_SAMPLE_FACTORS - PZOk_SP_12 Sample Type:Study Pool HHEAR-ECHO ID=EM-8V9K1-QCSP-012-12701; RAW_FILE_NAME=PZOk_SP_12 SUBJECT_SAMPLE_FACTORS - PZOk_SP_13 Sample Type:Study Pool HHEAR-ECHO ID=EM-CL5X7-QCSP-013-12701; RAW_FILE_NAME=PZOk_SP_13 SUBJECT_SAMPLE_FACTORS - PZOk_SP_14 Sample Type:Study Pool HHEAR-ECHO ID=EM-HX545-QCSP-014-12701; RAW_FILE_NAME=PZOk_SP_14 SUBJECT_SAMPLE_FACTORS - PZOk_SP_15 Sample Type:Study Pool HHEAR-ECHO ID=EM-GW606-QCSP-015-12701; RAW_FILE_NAME=PZOk_SP_15 SUBJECT_SAMPLE_FACTORS - PZOk_SP_16 Sample Type:Study Pool HHEAR-ECHO ID=EM-YY108-QCSP-016-12701; RAW_FILE_NAME=PZOk_SP_16 SUBJECT_SAMPLE_FACTORS - PZOk_SP_17 Sample Type:Study Pool HHEAR-ECHO ID=EM-8EEC4-QCSP-017-12701; RAW_FILE_NAME=PZOk_SP_17 SUBJECT_SAMPLE_FACTORS - PZOk_SP_18 Sample Type:Study Pool HHEAR-ECHO ID=EM-8FAG7-QCSP-018-12701; RAW_FILE_NAME=PZOk_SP_18 SUBJECT_SAMPLE_FACTORS - PZOk_SP_19 Sample Type:Study Pool HHEAR-ECHO ID=EM-BK9G4-QCSP-019-12701; RAW_FILE_NAME=PZOk_SP_19 SUBJECT_SAMPLE_FACTORS - PZOk_SP_2 Sample Type:Study Pool HHEAR-ECHO ID=EM-88D13-QCSP-002-12701; RAW_FILE_NAME=PZOk_SP_2 SUBJECT_SAMPLE_FACTORS - PZOk_SP_20 Sample Type:Study Pool HHEAR-ECHO ID=EM-NH8M7-QCSP-020-12701; RAW_FILE_NAME=PZOk_SP_20 SUBJECT_SAMPLE_FACTORS - PZOk_SP_21 Sample Type:Study Pool HHEAR-ECHO ID=EM-Z3FN4-QCSP-021-12701; RAW_FILE_NAME=PZOk_SP_21 SUBJECT_SAMPLE_FACTORS - PZOk_SP_22 Sample Type:Study Pool HHEAR-ECHO ID=EM-6A2C4-QCSP-022-12701; RAW_FILE_NAME=PZOk_SP_22 SUBJECT_SAMPLE_FACTORS - PZOk_SP_23 Sample Type:Study Pool HHEAR-ECHO ID=EM-3BZ63-QCSP-023-12701; RAW_FILE_NAME=PZOk_SP_23 SUBJECT_SAMPLE_FACTORS - PZOk_SP_24 Sample Type:Study Pool HHEAR-ECHO ID=EM-HGMJ0-QCSP-024-12701; RAW_FILE_NAME=PZOk_SP_24 SUBJECT_SAMPLE_FACTORS - PZOk_SP_25 Sample Type:Study Pool HHEAR-ECHO ID=EM-XTJP5-QCSP-025-12701; RAW_FILE_NAME=PZOk_SP_25 SUBJECT_SAMPLE_FACTORS - PZOk_SP_26 Sample Type:Study Pool HHEAR-ECHO ID=EM-DYX26-QCSP-026-12701; RAW_FILE_NAME=PZOk_SP_26 SUBJECT_SAMPLE_FACTORS - PZOk_SP_27 Sample Type:Study Pool HHEAR-ECHO ID=EM-45NV2-QCSP-027-12701; RAW_FILE_NAME=PZOk_SP_27 SUBJECT_SAMPLE_FACTORS - PZOk_SP_28 Sample Type:Study Pool HHEAR-ECHO ID=EM-J8TF3-QCSP-028-12701; RAW_FILE_NAME=PZOk_SP_28 SUBJECT_SAMPLE_FACTORS - PZOk_SP_29 Sample Type:Study Pool HHEAR-ECHO ID=EM-XNGX3-QCSP-029-12701; RAW_FILE_NAME=PZOk_SP_29 SUBJECT_SAMPLE_FACTORS - PZOk_SP_3 Sample Type:Study Pool HHEAR-ECHO ID=EM-2JEQ8-QCSP-003-12701; RAW_FILE_NAME=PZOk_SP_3 SUBJECT_SAMPLE_FACTORS - PZOk_SP_30 Sample Type:Study Pool HHEAR-ECHO ID=EM-7TMF7-QCSP-030-12701; RAW_FILE_NAME=PZOk_SP_30 SUBJECT_SAMPLE_FACTORS - PZOk_SP_31 Sample Type:Study Pool HHEAR-ECHO ID=EM-38LL1-QCSP-031-12701; RAW_FILE_NAME=PZOk_SP_31 SUBJECT_SAMPLE_FACTORS - PZOk_SP_32 Sample Type:Study Pool HHEAR-ECHO ID=EM-F5V09-QCSP-032-12701; RAW_FILE_NAME=PZOk_SP_32 SUBJECT_SAMPLE_FACTORS - PZOk_SP_33 Sample Type:Study Pool HHEAR-ECHO ID=EM-5MHW3-QCSP-033-12701; RAW_FILE_NAME=PZOk_SP_33 SUBJECT_SAMPLE_FACTORS - PZOk_SP_34 Sample Type:Study Pool HHEAR-ECHO ID=EM-U48H5-QCSP-034-12701; RAW_FILE_NAME=PZOk_SP_34 SUBJECT_SAMPLE_FACTORS - PZOk_SP_35 Sample Type:Study Pool HHEAR-ECHO ID=EM-TPYD9-QCSP-035-12701; RAW_FILE_NAME=PZOk_SP_35 SUBJECT_SAMPLE_FACTORS - PZOk_SP_36 Sample Type:Study Pool HHEAR-ECHO ID=EM-717M1-QCSP-036-12701; RAW_FILE_NAME=PZOk_SP_36 SUBJECT_SAMPLE_FACTORS - PZOk_SP_37 Sample Type:Study Pool HHEAR-ECHO ID=EM-46PT2-QCSP-037-12701; RAW_FILE_NAME=PZOk_SP_37 SUBJECT_SAMPLE_FACTORS - PZOk_SP_38 Sample Type:Study Pool HHEAR-ECHO ID=EM-8H901-QCSP-038-12701; RAW_FILE_NAME=PZOk_SP_38 SUBJECT_SAMPLE_FACTORS - PZOk_SP_39 Sample Type:Study Pool HHEAR-ECHO ID=EM-M2D23-QCSP-039-12701; RAW_FILE_NAME=PZOk_SP_39 SUBJECT_SAMPLE_FACTORS - PZOk_SP_4 Sample Type:Study Pool HHEAR-ECHO ID=EM-870F9-QCSP-004-12701; RAW_FILE_NAME=PZOk_SP_4 SUBJECT_SAMPLE_FACTORS - PZOk_SP_40 Sample Type:Study Pool HHEAR-ECHO ID=EM-GQZC2-QCSP-040-12701; RAW_FILE_NAME=PZOk_SP_40 SUBJECT_SAMPLE_FACTORS - PZOk_SP_41 Sample Type:Study Pool HHEAR-ECHO ID=EM-SXL58-QCSP-041-12701; RAW_FILE_NAME=PZOk_SP_41 SUBJECT_SAMPLE_FACTORS - PZOk_SP_42 Sample Type:Study Pool HHEAR-ECHO ID=EM-CYCW7-QCSP-042-12701; RAW_FILE_NAME=PZOk_SP_42 SUBJECT_SAMPLE_FACTORS - PZOk_SP_43 Sample Type:Study Pool HHEAR-ECHO ID=EM-FFNU9-QCSP-043-12701; RAW_FILE_NAME=PZOk_SP_43 SUBJECT_SAMPLE_FACTORS - PZOk_SP_44 Sample Type:Study Pool HHEAR-ECHO ID=EM-RV9Y4-QCSP-044-12701; RAW_FILE_NAME=PZOk_SP_44 SUBJECT_SAMPLE_FACTORS - PZOk_SP_45 Sample Type:Study Pool HHEAR-ECHO ID=EM-2YPS9-QCSP-045-12701; RAW_FILE_NAME=PZOk_SP_45 SUBJECT_SAMPLE_FACTORS - PZOk_SP_46 Sample Type:Study Pool HHEAR-ECHO ID=EM-R8SY4-QCSP-046-12701; RAW_FILE_NAME=PZOk_SP_46 SUBJECT_SAMPLE_FACTORS - PZOk_SP_47 Sample Type:Study Pool HHEAR-ECHO ID=EM-ECM09-QCSP-047-12701; RAW_FILE_NAME=PZOk_SP_47 SUBJECT_SAMPLE_FACTORS - PZOk_SP_48 Sample Type:Study Pool HHEAR-ECHO ID=EM-4QAT8-QCSP-048-12701; RAW_FILE_NAME=PZOk_SP_48 SUBJECT_SAMPLE_FACTORS - PZOk_SP_49 Sample Type:Study Pool HHEAR-ECHO ID=EM-JQF26-QCSP-049-12701; RAW_FILE_NAME=PZOk_SP_49 SUBJECT_SAMPLE_FACTORS - PZOk_SP_5 Sample Type:Study Pool HHEAR-ECHO ID=EM-QN1G0-QCSP-005-12701; RAW_FILE_NAME=PZOk_SP_5 SUBJECT_SAMPLE_FACTORS - PZOk_SP_50 Sample Type:Study Pool HHEAR-ECHO ID=EM-4SUH0-QCSP-050-12701; RAW_FILE_NAME=PZOk_SP_50 SUBJECT_SAMPLE_FACTORS - PZOk_SP_51 Sample Type:Study Pool HHEAR-ECHO ID=EM-NG5D1-QCSP-051-12701; RAW_FILE_NAME=PZOk_SP_51 SUBJECT_SAMPLE_FACTORS - PZOk_SP_52 Sample Type:Study Pool HHEAR-ECHO ID=EM-V1ZW3-QCSP-052-12701; RAW_FILE_NAME=PZOk_SP_52 SUBJECT_SAMPLE_FACTORS - PZOk_SP_53 Sample Type:Study Pool HHEAR-ECHO ID=EM-PUPA8-QCSP-053-12701; RAW_FILE_NAME=PZOk_SP_53 SUBJECT_SAMPLE_FACTORS - PZOk_SP_54 Sample Type:Study Pool HHEAR-ECHO ID=EM-GNWU1-QCSP-054-12701; RAW_FILE_NAME=PZOk_SP_54 SUBJECT_SAMPLE_FACTORS - PZOk_SP_55 Sample Type:Study Pool HHEAR-ECHO ID=EM-YPRU4-QCSP-055-12701; RAW_FILE_NAME=PZOk_SP_55 SUBJECT_SAMPLE_FACTORS - PZOk_SP_56 Sample Type:Study Pool HHEAR-ECHO ID=EM-YWCX7-QCSP-056-12701; RAW_FILE_NAME=PZOk_SP_56 SUBJECT_SAMPLE_FACTORS - PZOk_SP_57 Sample Type:Study Pool HHEAR-ECHO ID=EM-R3ZH6-QCSP-057-12701; RAW_FILE_NAME=PZOk_SP_57 SUBJECT_SAMPLE_FACTORS - PZOk_SP_58 Sample Type:Study Pool HHEAR-ECHO ID=EM-C8SW4-QCSP-058-12701; RAW_FILE_NAME=PZOk_SP_58 SUBJECT_SAMPLE_FACTORS - PZOk_SP_59 Sample Type:Study Pool HHEAR-ECHO ID=EM-L6777-QCSP-059-12701; RAW_FILE_NAME=PZOk_SP_59 SUBJECT_SAMPLE_FACTORS - PZOk_SP_6 Sample Type:Study Pool HHEAR-ECHO ID=EM-UGJH5-QCSP-006-12701; RAW_FILE_NAME=PZOk_SP_6 SUBJECT_SAMPLE_FACTORS - PZOk_SP_60 Sample Type:Study Pool HHEAR-ECHO ID=EM-TPP85-QCSP-060-12701; 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RAW_FILE_NAME=PZOk_S_946 SUBJECT_SAMPLE_FACTORS - PZOk_S_947 Sample Type:Study Sample HHEAR-ECHO ID=EM-D7YH2-P-01-12701; RAW_FILE_NAME=PZOk_S_947 SUBJECT_SAMPLE_FACTORS - PZOk_S_948 Sample Type:Study Sample HHEAR-ECHO ID=EM-R6K22-P-01-12701; RAW_FILE_NAME=PZOk_S_948 SUBJECT_SAMPLE_FACTORS - PZOk_S_949 Sample Type:Study Sample HHEAR-ECHO ID=EM-AWG88-P-01-12701; RAW_FILE_NAME=PZOk_S_949 SUBJECT_SAMPLE_FACTORS - PZOk_S_95 Sample Type:Study Sample HHEAR-ECHO ID=EM-A3CJ1-P-01-12701; RAW_FILE_NAME=PZOk_S_95 SUBJECT_SAMPLE_FACTORS - PZOk_S_950 Sample Type:Study Sample HHEAR-ECHO ID=EM-B4E45-P-01-12701; RAW_FILE_NAME=PZOk_S_950 SUBJECT_SAMPLE_FACTORS - PZOk_S_951 Sample Type:Study Sample HHEAR-ECHO ID=EM-QUCX9-P-01-12701; RAW_FILE_NAME=PZOk_S_951 SUBJECT_SAMPLE_FACTORS - PZOk_S_952 Sample Type:Study Sample HHEAR-ECHO ID=EM-YMMC6-P-01-12701; RAW_FILE_NAME=PZOk_S_952 SUBJECT_SAMPLE_FACTORS - PZOk_S_953 Sample Type:Study Sample HHEAR-ECHO ID=EM-CGPA2-P-01-12701; RAW_FILE_NAME=PZOk_S_953 SUBJECT_SAMPLE_FACTORS - PZOk_S_954 Sample Type:Study Sample HHEAR-ECHO ID=EM-7ZAP4-P-01-12701; RAW_FILE_NAME=PZOk_S_954 SUBJECT_SAMPLE_FACTORS - PZOk_S_955 Sample Type:Study Sample HHEAR-ECHO ID=EM-R35A8-P-01-12701; RAW_FILE_NAME=PZOk_S_955 SUBJECT_SAMPLE_FACTORS - PZOk_S_956 Sample Type:Study Sample HHEAR-ECHO ID=EM-4PCG7-P-01-12701; RAW_FILE_NAME=PZOk_S_956 SUBJECT_SAMPLE_FACTORS - PZOk_S_957 Sample Type:Study Sample HHEAR-ECHO ID=EM-P6LE1-P-01-12701; RAW_FILE_NAME=PZOk_S_957 SUBJECT_SAMPLE_FACTORS - PZOk_S_958 Sample Type:Study Sample HHEAR-ECHO ID=EM-C1M75-P-01-12701; RAW_FILE_NAME=PZOk_S_958 SUBJECT_SAMPLE_FACTORS - PZOk_S_96 Sample Type:Study Sample HHEAR-ECHO ID=EM-S8QD1-P-01-12701; RAW_FILE_NAME=PZOk_S_96 SUBJECT_SAMPLE_FACTORS - PZOk_S_961 Sample Type:Study Sample HHEAR-ECHO ID=EM-BSZ52-P-01-12701; RAW_FILE_NAME=PZOk_S_961 SUBJECT_SAMPLE_FACTORS - PZOk_S_962 Sample Type:Study Sample HHEAR-ECHO ID=EM-Z1FE8-P-01-12701; RAW_FILE_NAME=PZOk_S_962 SUBJECT_SAMPLE_FACTORS - PZOk_S_963 Sample Type:Study Sample HHEAR-ECHO ID=EM-R00C5-P-01-12701; RAW_FILE_NAME=PZOk_S_963 SUBJECT_SAMPLE_FACTORS - PZOk_S_964 Sample Type:Study Sample HHEAR-ECHO ID=EM-F3J30-P-01-12701; RAW_FILE_NAME=PZOk_S_964 SUBJECT_SAMPLE_FACTORS - PZOk_S_965 Sample Type:Study Sample HHEAR-ECHO ID=EM-PGLJ3-P-01-12701; RAW_FILE_NAME=PZOk_S_965 SUBJECT_SAMPLE_FACTORS - PZOk_S_966 Sample Type:Study Sample HHEAR-ECHO ID=EM-UGJL6-P-01-12701; RAW_FILE_NAME=PZOk_S_966 SUBJECT_SAMPLE_FACTORS - PZOk_S_967 Sample Type:Study Sample HHEAR-ECHO ID=EM-BWNX9-P-01-12701; RAW_FILE_NAME=PZOk_S_967 SUBJECT_SAMPLE_FACTORS - PZOk_S_968 Sample Type:Study Sample HHEAR-ECHO ID=EM-CQ918-P-01-12701; RAW_FILE_NAME=PZOk_S_968 SUBJECT_SAMPLE_FACTORS - PZOk_S_969 Sample Type:Study Sample HHEAR-ECHO ID=EM-U7RT5-P-01-12701; RAW_FILE_NAME=PZOk_S_969 SUBJECT_SAMPLE_FACTORS - PZOk_S_97 Sample Type:Study Sample HHEAR-ECHO ID=EM-TRQ49-P-01-12701; RAW_FILE_NAME=PZOk_S_97 SUBJECT_SAMPLE_FACTORS - PZOk_S_970 Sample Type:Study Sample HHEAR-ECHO ID=EM-UL6E0-P-01-12701; RAW_FILE_NAME=PZOk_S_970 SUBJECT_SAMPLE_FACTORS - PZOk_S_971 Sample Type:Study Sample HHEAR-ECHO ID=EM-KTW96-P-01-12701; RAW_FILE_NAME=PZOk_S_971 SUBJECT_SAMPLE_FACTORS - PZOk_S_972 Sample Type:Study Sample HHEAR-ECHO ID=EM-Q3DD7-P-01-12701; RAW_FILE_NAME=PZOk_S_972 SUBJECT_SAMPLE_FACTORS - PZOk_S_973 Sample Type:Study Sample HHEAR-ECHO ID=EM-YS0F4-P-01-12701; RAW_FILE_NAME=PZOk_S_973 SUBJECT_SAMPLE_FACTORS - PZOk_S_974 Sample Type:Study Sample HHEAR-ECHO ID=EM-DLLG1-P-01-12701; RAW_FILE_NAME=PZOk_S_974 SUBJECT_SAMPLE_FACTORS - PZOk_S_975 Sample Type:Study Sample HHEAR-ECHO ID=EM-HJAN6-P-01-12701; RAW_FILE_NAME=PZOk_S_975 SUBJECT_SAMPLE_FACTORS - PZOk_S_976 Sample Type:Study Sample HHEAR-ECHO ID=EM-3Y7L9-P-01-12701; RAW_FILE_NAME=PZOk_S_976 SUBJECT_SAMPLE_FACTORS - PZOk_S_977 Sample Type:Study Sample HHEAR-ECHO ID=EM-KYWY4-P-01-12701; RAW_FILE_NAME=PZOk_S_977 SUBJECT_SAMPLE_FACTORS - PZOk_S_978 Sample Type:Study Sample HHEAR-ECHO ID=EM-LTR33-P-01-12701; RAW_FILE_NAME=PZOk_S_978 SUBJECT_SAMPLE_FACTORS - PZOk_S_979 Sample Type:Study Sample HHEAR-ECHO ID=EM-YV2G4-P-01-12701; RAW_FILE_NAME=PZOk_S_979 SUBJECT_SAMPLE_FACTORS - PZOk_S_98 Sample Type:Study Sample HHEAR-ECHO ID=EM-CL3Q5-P-01-12701; RAW_FILE_NAME=PZOk_S_98 SUBJECT_SAMPLE_FACTORS - PZOk_S_980 Sample Type:Study Sample HHEAR-ECHO ID=EM-YYCD6-P-01-12701; RAW_FILE_NAME=PZOk_S_980 SUBJECT_SAMPLE_FACTORS - PZOk_S_981 Sample Type:Study Sample HHEAR-ECHO ID=EM-8KX37-P-01-12701; RAW_FILE_NAME=PZOk_S_981 SUBJECT_SAMPLE_FACTORS - PZOk_S_982 Sample Type:Study Sample HHEAR-ECHO ID=EM-MZXU8-P-01-12701; RAW_FILE_NAME=PZOk_S_982 SUBJECT_SAMPLE_FACTORS - PZOk_S_983 Sample Type:Study Sample HHEAR-ECHO ID=EM-Z6641-P-01-12701; RAW_FILE_NAME=PZOk_S_983 SUBJECT_SAMPLE_FACTORS - PZOk_S_984 Sample Type:Study Sample HHEAR-ECHO ID=EM-81P73-P-01-12701; RAW_FILE_NAME=PZOk_S_984 SUBJECT_SAMPLE_FACTORS - PZOk_S_985 Sample Type:Study Sample HHEAR-ECHO ID=EM-51A48-P-01-12701; RAW_FILE_NAME=PZOk_S_985 SUBJECT_SAMPLE_FACTORS - PZOk_S_986 Sample Type:Study Sample HHEAR-ECHO ID=EM-B79E2-P-01-12701; RAW_FILE_NAME=PZOk_S_986 SUBJECT_SAMPLE_FACTORS - PZOk_S_987 Sample Type:Study Sample HHEAR-ECHO ID=EM-CLA29-P-01-12701; RAW_FILE_NAME=PZOk_S_987 SUBJECT_SAMPLE_FACTORS - PZOk_S_988 Sample Type:Study Sample HHEAR-ECHO ID=EM-5HN78-P-01-12701; RAW_FILE_NAME=PZOk_S_988 SUBJECT_SAMPLE_FACTORS - PZOk_S_989 Sample Type:Study Sample HHEAR-ECHO ID=EM-R4JE6-P-01-12701; RAW_FILE_NAME=PZOk_S_989 SUBJECT_SAMPLE_FACTORS - PZOk_S_99 Sample Type:Study Sample HHEAR-ECHO ID=EM-WRJP1-P-01-12701; RAW_FILE_NAME=PZOk_S_99 SUBJECT_SAMPLE_FACTORS - PZOk_S_990 Sample Type:Study Sample HHEAR-ECHO ID=EM-HJYC7-P-01-12701; RAW_FILE_NAME=PZOk_S_990 SUBJECT_SAMPLE_FACTORS - PZOk_S_991 Sample Type:Study Sample HHEAR-ECHO ID=EM-Z0HL3-P-01-12701; RAW_FILE_NAME=PZOk_S_991 SUBJECT_SAMPLE_FACTORS - PZOk_S_992 Sample Type:Study Sample HHEAR-ECHO ID=EM-B7JE5-P-01-12701; RAW_FILE_NAME=PZOk_S_992 SUBJECT_SAMPLE_FACTORS - PZOk_S_993 Sample Type:Study Sample HHEAR-ECHO ID=EM-DWVA0-P-01-12701; RAW_FILE_NAME=PZOk_S_993 SUBJECT_SAMPLE_FACTORS - PZOk_S_994 Sample Type:Study Sample HHEAR-ECHO ID=EM-T7JM0-P-01-12701; RAW_FILE_NAME=PZOk_S_994 SUBJECT_SAMPLE_FACTORS - PZOk_S_995 Sample Type:Study Sample HHEAR-ECHO ID=EM-JF6H8-P-01-12701; RAW_FILE_NAME=PZOk_S_995 SUBJECT_SAMPLE_FACTORS - PZOk_S_996 Sample Type:Study Sample HHEAR-ECHO ID=EM-9YGS1-P-01-12701; RAW_FILE_NAME=PZOk_S_996 SUBJECT_SAMPLE_FACTORS - PZOk_S_997 Sample Type:Study Sample HHEAR-ECHO ID=EM-5DMV9-P-01-12701; RAW_FILE_NAME=PZOk_S_997 SUBJECT_SAMPLE_FACTORS - PZOk_S_998 Sample Type:Study Sample HHEAR-ECHO ID=EM-Y1JB2-P-01-12701; RAW_FILE_NAME=PZOk_S_998 SUBJECT_SAMPLE_FACTORS - PZOk_S_999 Sample Type:Study Sample HHEAR-ECHO ID=EM-LXGN5-P-01-12701; RAW_FILE_NAME=PZOk_S_999 #COLLECTION CO:COLLECTION_SUMMARY Project Viva: Pregnant women were enrolled in Project Viva between 1999 and 2002 CO:COLLECTION_SUMMARY at their first prenatal visit at one of 8 obstetric clinics of Atrius Harvard CO:COLLECTION_SUMMARY Vanguard Medical Associates, a multispecialty group practice in eastern CO:COLLECTION_SUMMARY Massachusetts. Eligible mothers were fluent in English, had singleton CO:COLLECTION_SUMMARY gestations, were <22 weeks gestation, and had no plans to move away from the CO:COLLECTION_SUMMARY study area. Research staff performed in-person study visits with participating CO:COLLECTION_SUMMARY mothers in the first (median gestational age 9.9 weeks) and second (median CO:COLLECTION_SUMMARY gestational age 28.1 weeks) trimesters of pregnancy, and with mothers and CO:COLLECTION_SUMMARY children during the first few days after delivery, during infancy (median age CO:COLLECTION_SUMMARY 6.3 months), in early childhood (median age 3.3 years), mid-childhood (median CO:COLLECTION_SUMMARY age 7.7 years), and adolescence (median age13 years). In this analysis, we will CO:COLLECTION_SUMMARY use data from 188 mother-child pairs in Project Viva with available information CO:COLLECTION_SUMMARY on prenatal PFAS concentrations, available umbilical cord serum samples at CO:COLLECTION_SUMMARY delivery, and outcomes of interest. Please contact MollyAn Killingbeck at CO:COLLECTION_SUMMARY mollyan.killingbeck@point32health.org for questions related to the subject CO:COLLECTION_SUMMARY characteristics and outcomes. CO:SAMPLE_TYPE Blood (plasma) CO:STORAGE_CONDITIONS -80℃ #TREATMENT TR:TREATMENT_SUMMARY N/A #SAMPLEPREP SP:SAMPLEPREP_SUMMARY Brigham and Women’s Hospital Channing aliquoted the study samples (50 µL) and SP:SAMPLEPREP_SUMMARY made the total study pool sample according to the statement of work provided by SP:SAMPLEPREP_SUMMARY UNC. These samples were shipped to the NC HHEAR Hub on dry ice. Before sample SP:SAMPLEPREP_SUMMARY preparation, the NC HHEAR Hub thawed and mixed the total study pool samples and SP:SAMPLEPREP_SUMMARY distributed it with 50 µL per aliquot, which were used as QC study pools SP:SAMPLEPREP_SUMMARY throughout the whole study. HHEAR reference plasma (50 µL each) and NIST plasma SP:SAMPLEPREP_SUMMARY (1950) reference material (50 µL each) were provided by the NC HHEAR Hub. The SP:SAMPLEPREP_SUMMARY LC-MS grade water (50 µL) was used as blank. Sample preparation was conducted SP:SAMPLEPREP_SUMMARY by batch and all samples were thawed at 4°C overnight before the preparation. SP:SAMPLEPREP_SUMMARY Samples, including study samples, study pool samples, HHEAR reference plasma, SP:SAMPLEPREP_SUMMARY NIST reference plasma, and blanks were mixed with 400 µL methanol containing SP:SAMPLEPREP_SUMMARY 500 ng/mL tryptophan-d5 as internal standard and vortexed by a multiple tube SP:SAMPLEPREP_SUMMARY vortex mixer for 2 min at 5000 rpm at room temperature. All samples were SP:SAMPLEPREP_SUMMARY centrifuged at 16,000 rcf for 10 min at 4°C. The supernatant (350 µL) was SP:SAMPLEPREP_SUMMARY transferred into a pre-labeled 2.0 mL Lo-bind Eppendorf tube, dried by a SP:SAMPLEPREP_SUMMARY SpeedVac overnight, and stored at -80° C. For immediate analysis, 100 µL of SP:SAMPLEPREP_SUMMARY water-methanol solution (95:5, v/v) was used to reconstitute the dried extracts. SP:SAMPLEPREP_SUMMARY Samples were thoroughly mixed on a multiple tube vortex mixer for 10 min at 5000 SP:SAMPLEPREP_SUMMARY rpm at room temperature and then centrifuged at 4°C for 10 min at 16,000 rcf. SP:SAMPLEPREP_SUMMARY The supernatant was transferred to pre-labeled autosampler vials for data SP:SAMPLEPREP_SUMMARY acquisition by LC-MS. SP:PROCESSING_STORAGE_CONDITIONS On ice SP:EXTRACT_STORAGE Described in summary #CHROMATOGRAPHY CH:CHROMATOGRAPHY_SUMMARY Reverse phase CH:INSTRUMENT_NAME Thermo Scientific™ Vanquish™ UPHPLC CH:COLUMN_NAME Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,3um) CH:COLUMN_PRESSURE 6000-10000 CH:COLUMN_TEMPERATURE 50 CH:FLOW_GRADIENT Time(min) Flow Rate %A %B Curve 1. 0 0.4 99.0 1.0 5 2. 1.00 0.4 99.0 1.0 5 3. CH:FLOW_GRADIENT 16.00 0.4 1.0 99.0 5 4. 19.00 0.4 1.0 99.0 5 5. 19.50 0.4 99.0 1.0 5 6. 22.00 CH:FLOW_GRADIENT 0.4 99.0 1.0 5 CH:FLOW_RATE 0.4 ml/min CH:INJECTION_TEMPERATURE 8 CH:SOLVENT_A 100% water; 0.1% formic acid CH:SOLVENT_B 100% methanol; 0.1% formic acid CH:RANDOMIZATION_ORDER Randomized CH:CHROMATOGRAPHY_TYPE Reversed phase #ANALYSIS AN:LABORATORY_NAME UNC-NRI Sumner/Li lab AN:ANALYSIS_TYPE MS AN:SOFTWARE_VERSION Xcalibur 4.1.31.9 AN:OPERATOR_NAME Madison Schroder AN:DETECTOR_TYPE Orbitrap AN:DATA_FORMAT Profile #MS MS:INSTRUMENT_NAME Thermo Q Exactive HF-X Orbitrap MS:INSTRUMENT_TYPE Orbitrap MS:MS_TYPE ESI MS:MS_COMMENTS Instrument: Thermo Q Exactive HFx Software: Xcalibur 4.1.31.9 for data MS:MS_COMMENTS acquisition; Progenesis QI 2.4 for data preprocessing MS:ION_MODE POSITIVE MS:CAPILLARY_TEMPERATURE 320 °C MS:CAPILLARY_VOLTAGE 3.5 KV MS:COLLISION_ENERGY 20-45, ramp MS:COLLISION_GAS N2 MS:DRY_GAS_FLOW 55 MS:DRY_GAS_TEMP 400°C MS:FRAGMENTATION_METHOD CID MS:DESOLVATION_GAS_FLOW 55 MS:MS_RESULTS_FILE ST002336_AN003818_Results.txt UNITS:Normalized intensity Has m/z:Yes Has RT:Yes RT units:Minutes #END