#METABOLOMICS WORKBENCH mlhomme_20221213_043543 DATATRACK_ID:3642 STUDY_ID:ST002400 ANALYSIS_ID:AN003911
VERSION                          	1
CREATED_ON                       	12-16-2022
#PROJECT
PR:PROJECT_TITLE                 	Alcohol dehydrogenase 1B is crucial for adipocyte homeostasis.
PR:PROJECT_SUMMARY               	Background. Alcohol dehydrogenase (ADH1B), encoded by the ADH1B gene, is a
PR:PROJECT_SUMMARY               	cytosolic enzyme mainly known for its role in ethanol catabolism in the liver. A
PR:PROJECT_SUMMARY               	few studies have paved the way to show an equally important role of ADH1B in
PR:PROJECT_SUMMARY               	adipocytes. This study aimed to better identify the cellular mechanisms and
PR:PROJECT_SUMMARY               	signaling pathways involving ADH1B in adipose tissue and to determine if ADH1B
PR:PROJECT_SUMMARY               	variants might contribute to adipose tissue dysfunction. Results. We showed that
PR:PROJECT_SUMMARY               	CRISPR-Cas9-mediated ADH1B knockout (KO) in human adipose stem cells (ASC)
PR:PROJECT_SUMMARY               	abolished adipocyte differentiation and decreased insulin response. This was
PR:PROJECT_SUMMARY               	accompanied by oxidative stress, altered mitochondrial functions, and cellular
PR:PROJECT_SUMMARY               	senescence. Lipidomic analysis revealed that ADH1B deficiency results in a major
PR:PROJECT_SUMMARY               	remodeling of lipid composition in ASC. An ADH1B homozygous loss-of-function
PR:PROJECT_SUMMARY               	variant was also identified in a patient presenting with a lipodystrophic and
PR:PROJECT_SUMMARY               	insulin resistant syndrome associated with major liver dysfunction, leading to
PR:PROJECT_SUMMARY               	early death. Discussion. This translational study underlines the crucial role of
PR:PROJECT_SUMMARY               	ADH1B in adipose tissue. It unveils cellular mechanisms accounting for its key
PR:PROJECT_SUMMARY               	role in adipogenesis, and adipocyte homeostasis. This study also identifies
PR:PROJECT_SUMMARY               	ADH1B as a candidate gene in monogenic forms of lipodystrophic and insulin
PR:PROJECT_SUMMARY               	resistant syndromes.
PR:INSTITUTE                     	INSERM
PR:LAST_NAME                     	Gautheron
PR:FIRST_NAME                    	Jérémie
PR:ADDRESS                       	27 rue Chaligny, 75012 Paris France
PR:EMAIL                         	jeremie.gautheron@gmail.com
PR:PHONE                         	+33623398373
PR:DOI                           	http://dx.doi.org/10.21228/M8VM64
#STUDY
ST:STUDY_TITLE                   	Alcohol dehydrogenase 1B is crucial for adipocyte homeostasis
ST:STUDY_SUMMARY                 	Background. Alcohol dehydrogenase (ADH1B), encoded by the ADH1B gene, is a
ST:STUDY_SUMMARY                 	cytosolic enzyme mainly known for its role in ethanol catabolism in the liver. A
ST:STUDY_SUMMARY                 	few studies have paved the way to show an equally important role of ADH1B in
ST:STUDY_SUMMARY                 	adipocytes. This study aimed to better identify the cellular mechanisms and
ST:STUDY_SUMMARY                 	signaling pathways involving ADH1B in adipose tissue and to determine if ADH1B
ST:STUDY_SUMMARY                 	variants might contribute to adipose tissue dysfunction. Results. We showed that
ST:STUDY_SUMMARY                 	CRISPR-Cas9-mediated ADH1B knockout (KO) in human adipose stem cells (ASC)
ST:STUDY_SUMMARY                 	abolished adipocyte differentiation and decreased insulin response. This was
ST:STUDY_SUMMARY                 	accompanied by oxidative stress, altered mitochondrial functions, and cellular
ST:STUDY_SUMMARY                 	senescence. Lipidomic analysis revealed that ADH1B deficiency results in a major
ST:STUDY_SUMMARY                 	remodeling of lipid composition in ASC. An ADH1B homozygous loss-of-function
ST:STUDY_SUMMARY                 	variant was also identified in a patient presenting with a lipodystrophic and
ST:STUDY_SUMMARY                 	insulin resistant syndrome associated with major liver dysfunction, leading to
ST:STUDY_SUMMARY                 	early death. Discussion. This translational study underlines the crucial role of
ST:STUDY_SUMMARY                 	ADH1B in adipose tissue. It unveils cellular mechanisms accounting for its key
ST:STUDY_SUMMARY                 	role in adipogenesis, and adipocyte homeostasis. This study also identifies
ST:STUDY_SUMMARY                 	ADH1B as a candidate gene in monogenic forms of lipodystrophic and insulin
ST:STUDY_SUMMARY                 	resistant syndromes.
ST:INSTITUTE                     	INSERM
ST:LAST_NAME                     	Gautheron
ST:FIRST_NAME                    	Jérémie
ST:ADDRESS                       	27 rue Chaligny
ST:EMAIL                         	jeremie.gautheron@gmail.com
ST:PHONE                         	+33623398373
ST:SUBMIT_DATE                   	2022-12-13
#SUBJECT
SU:SUBJECT_TYPE                  	Cultured cells
SU:SUBJECT_SPECIES               	Homo sapiens
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data
SUBJECT_SAMPLE_FACTORS           	ADH1B_KO_01_LIP329	ADH1B_KO_01_LIP329	Genotype:ADH1B_KO	Batch=1; RAW_FILE_NAME=LIP329_C18_1x_KO_01; RAW_FILE_NAME=LIP329_C18_10x_KO_01; RAW_FILE_NAME=LIP329_long_1x_KO_01; RAW_FILE_NAME=LIP329_short_1x_KO_01; RAW_FILE_NAME=LIP329_short_20x_KO_01
SUBJECT_SAMPLE_FACTORS           	ADH1B_KO_02_LIP329	ADH1B_KO_02_LIP329	Genotype:ADH1B_KO	Batch=1; RAW_FILE_NAME=LIP329_C18_1x_KO_02; RAW_FILE_NAME=LIP329_C18_10x_KO_02; RAW_FILE_NAME=LIP329_long_1x_KO_02; RAW_FILE_NAME=LIP329_short_1x_KO_02; RAW_FILE_NAME=LIP329_short_20x_KO_02
SUBJECT_SAMPLE_FACTORS           	ADH1B_KO_03_LIP329	ADH1B_KO_03_LIP329	Genotype:ADH1B_KO	Batch=1; RAW_FILE_NAME=LIP329_C18_1x_KO_03; RAW_FILE_NAME=LIP329_C18_10x_KO_03; RAW_FILE_NAME=LIP329_long_1x_KO_03; RAW_FILE_NAME=LIP329_short_1x_KO_03; RAW_FILE_NAME=LIP329_short_20x_KO_03
SUBJECT_SAMPLE_FACTORS           	ADH1B_KO_04_LIP329	ADH1B_KO_04_LIP329	Genotype:ADH1B_KO	Batch=1; RAW_FILE_NAME=LIP329_C18_1x_KO_04; RAW_FILE_NAME=LIP329_C18_10x_KO_04; RAW_FILE_NAME=LIP329_long_1x_KO_04; RAW_FILE_NAME=LIP329_short_1x_KO_04; RAW_FILE_NAME=LIP329_short_20x_KO_04
SUBJECT_SAMPLE_FACTORS           	ADH1B_KO_05_LIP329	ADH1B_KO_05_LIP329	Genotype:ADH1B_KO	Batch=1; RAW_FILE_NAME=LIP329_C18_1x_KO_05; RAW_FILE_NAME=LIP329_C18_10x_KO_05; RAW_FILE_NAME=LIP329_long_1x_KO_05; RAW_FILE_NAME=LIP329_short_1x_KO_05; RAW_FILE_NAME=LIP329_short_20x_KO_05
SUBJECT_SAMPLE_FACTORS           	CTL_01_LIP329	CTL_01_LIP329	Genotype:CTL	Batch=1; RAW_FILE_NAME=LIP329_C18_1x_CTL_01; RAW_FILE_NAME=LIP329_C18_10x_CTL_01; RAW_FILE_NAME=LIP329_long_1x_CTL_01; RAW_FILE_NAME=LIP329_short_1x_CTL_01; RAW_FILE_NAME=LIP329_short_20x_CTL_01
SUBJECT_SAMPLE_FACTORS           	CTL_02_LIP329	CTL_02_LIP329	Genotype:CTL	Batch=1; RAW_FILE_NAME=LIP329_C18_1x_CTL_02; RAW_FILE_NAME=LIP329_C18_10x_CTL_02; RAW_FILE_NAME=LIP329_long_1x_CTL_02; RAW_FILE_NAME=LIP329_short_1x_CTL_02; RAW_FILE_NAME=LIP329_short_20x_CTL_02
SUBJECT_SAMPLE_FACTORS           	CTL_03_LIP329	CTL_03_LIP329	Genotype:CTL	Batch=1; RAW_FILE_NAME=LIP329_C18_1x_CTL_03; RAW_FILE_NAME=LIP329_C18_10x_CTL_03; RAW_FILE_NAME=LIP329_long_1x_CTL_03; RAW_FILE_NAME=LIP329_short_1x_CTL_03; RAW_FILE_NAME=LIP329_short_20x_CTL_03
SUBJECT_SAMPLE_FACTORS           	CTL_04_LIP329	CTL_04_LIP329	Genotype:CTL	Batch=1; RAW_FILE_NAME=LIP329_C18_1x_CTL_04; RAW_FILE_NAME=LIP329_C18_10x_CTL_04; RAW_FILE_NAME=LIP329_long_1x_CTL_04; RAW_FILE_NAME=LIP329_short_1x_CTL_04; RAW_FILE_NAME=LIP329_short_20x_CTL_04
SUBJECT_SAMPLE_FACTORS           	CTL_05_LIP329	CTL_05_LIP329	Genotype:CTL	Batch=1; RAW_FILE_NAME=LIP329_C18_1x_CTL_05; RAW_FILE_NAME=LIP329_C18_10x_CTL_05; RAW_FILE_NAME=LIP329_long_1x_CTL_05; RAW_FILE_NAME=LIP329_short_1x_CTL_05; RAW_FILE_NAME=LIP329_short_20x_CTL_05
#COLLECTION
CO:COLLECTION_SUMMARY            	ASC were isolated from surgical samples of subcutaneous abdominal adipose tissue
CO:COLLECTION_SUMMARY            	from a 25-year-old healthy woman with a normal body mass index (BMI). Adipose
CO:COLLECTION_SUMMARY            	tissue was enzymatically digested with collagenase B (0.2%).
CO:SAMPLE_TYPE                   	Adipose tissue
#TREATMENT
TR:TREATMENT_SUMMARY             	After centrifugation, stromal vascular fraction was filtered, rinsed, plated and
TR:TREATMENT_SUMMARY             	cultured in α-MEM with 10% Fetal Calf Serum (FCS), 1% GlutaMAX (#35050061,
TR:TREATMENT_SUMMARY             	Thermo Fisher Scientific), 1% Penicillin/streptomycin (PS - 10,000 UI/mL), 1%
TR:TREATMENT_SUMMARY             	HEPES and Fibroblast Growth Factor-2 (FGF-2 -145 nmol/L). After 24 h, only ASC
TR:TREATMENT_SUMMARY             	adhered to plastic surfaces, while other cells were removed after culture medium
TR:TREATMENT_SUMMARY             	replacement. ASC were maintained in an undifferentiated state in α-MEM
TR:TREATMENT_SUMMARY             	supplemented with 10 % newborn calf serum (#CA-1151500; Biosera, MI, USA), 1%
TR:TREATMENT_SUMMARY             	GlutaMAX, HEPES and P/S, and FGF-2 (145 nmol/L). Adipocyte differentiation was
TR:TREATMENT_SUMMARY             	induced by treating 2-day post-confluent cultures with high-glucose (25 mmol/L)
TR:TREATMENT_SUMMARY             	DMEM supplemented with 10 % FCS, 1 % PS, 1 µmol/L dexamethasone (#D4902;
TR:TREATMENT_SUMMARY             	Sigma-Aldrich, MI, USA), 1 µM rosiglitazone (#D4902; Sigma-Aldrich), 250 µM
TR:TREATMENT_SUMMARY             	3-isobutyl-1-methyl xanthine (IBMX) (#I7018; Sigma-Aldrich) and 0.17 µmol/L
TR:TREATMENT_SUMMARY             	insulin (#I0516; Sigma-Aldrich) for ten days. The medium was then replaced with
TR:TREATMENT_SUMMARY             	high-glucose DMEM supplemented with 10% FCS, 1 % PS, 1 µmol/L rosiglitazone and
TR:TREATMENT_SUMMARY             	0.17 µM insulin, and changed to fresh medium every 2 days until the 20th day.
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	Lipid extraction. Lipids were extracted from ASC cells according to a modified
SP:SAMPLEPREP_SUMMARY            	Folch method. Cell pellets were resuspended in 100µl methanol and supplemented
SP:SAMPLEPREP_SUMMARY            	with deuterated internal standards. Lipids were extracted with 1.5 mL chloroform
SP:SAMPLEPREP_SUMMARY            	and 650 µL methanol, sonicated for 15 min. Phase separation was triggered by
SP:SAMPLEPREP_SUMMARY            	addition of 450 µL of ammonium carbonate (250 mM). Lower organic phase was
SP:SAMPLEPREP_SUMMARY            	dried and resuspended in 200 µL of liquid chromatography – mass spectrometry
SP:SAMPLEPREP_SUMMARY            	(LC-MS) solvent.
#CHROMATOGRAPHY
CH:INSTRUMENT_NAME               	Shimadzu 20AD
CH:COLUMN_NAME                   	Merck Supelco Ascentis Express C18 (150 x 2.1mm,2.7um)
CH:COLUMN_TEMPERATURE            	43
CH:FLOW_GRADIENT                 	-
CH:FLOW_RATE                     	300ul/min
CH:SOLVENT_A                     	60% acetonitrile/40% water; 0.1% formic acid; 10mM ammonium formate
CH:SOLVENT_B                     	10% acetonitrile/90% isopropanol; 0.1% formic acid; 10mM ammonium formate
CH:CHROMATOGRAPHY_TYPE           	Reversed phase
#ANALYSIS
AN:ANALYSIS_TYPE                 	MS
#MS
MS:INSTRUMENT_NAME               	ABI Sciex 4000 QTrap
MS:INSTRUMENT_TYPE               	QTRAP
MS:MS_TYPE                       	ESI
MS:MS_COMMENTS                   	MRM acquisition of abundant neutral lipids: CE and TG after 10 fold dilution
MS:MS_COMMENTS                   	This acquisition is referred to as "C18_10x"
MS:ION_MODE                      	POSITIVE
#MS_METABOLITE_DATA
MS_METABOLITE_DATA:UNITS         	mol% of total lipids
MS_METABOLITE_DATA_START
Samples	ADH1B_KO_01_LIP329	ADH1B_KO_02_LIP329	ADH1B_KO_03_LIP329	ADH1B_KO_04_LIP329	ADH1B_KO_05_LIP329	CTL_01_LIP329	CTL_02_LIP329	CTL_03_LIP329	CTL_04_LIP329	CTL_05_LIP329
Factors	Genotype:ADH1B_KO	Genotype:ADH1B_KO	Genotype:ADH1B_KO	Genotype:ADH1B_KO	Genotype:ADH1B_KO	Genotype:CTL	Genotype:CTL	Genotype:CTL	Genotype:CTL	Genotype:CTL	
TG(48:0-16:0_32:0)	0.0221	0.0195	0.0211	0.0180	0.0185	0.0430	0.0297	0.0269	0.0188	0.0287
TG(48:1-16:1_32:0)	0.0064	0.0056	0.0066	0.0081	0.0073	0.0189	0.0127	0.0103	0.0077	0.0122
TG(48:1-18:1_30:0)	0.0040	0.0035	0.0043	0.0054	0.0048	0.0185	0.0117	0.0105	0.0078	0.0111
TG(48:2-14:1_34:1)	0.0002	0.0001	0.0002	0.0003	0.0002	0.0014	0.0009	0.0008	0.0005	0.0008
TG(48:2-16:0_32:2)	0.0017	0.0016	0.0019	0.0028	0.0026	0.0074	0.0046	0.0038	0.0028	0.0044
TG(48:2-18:1_30:1)	0.0009	0.0008	0.0010	0.0014	0.0014	0.0059	0.0036	0.0031	0.0024	0.0035
TG(48:2-18:2_30:0)	0.0007	0.0006	0.0008	0.0011	0.0009	0.0027	0.0016	0.0014	0.0009	0.0015
TG(48:3-14:0_34:3)	0.0002	0.0002	0.0002	0.0003	0.0003	0.0006	0.0004	0.0003	0.0002	0.0003
TG(48:3-16:1_32:2)	0.0006	0.0007	0.0008	0.0010	0.0009	0.0027	0.0017	0.0015	0.0011	0.0017
TG(49:1-14:0_35:1)	0.0006	0.0004	0.0005	0.0007	0.0007	0.0018	0.0011	0.0009	0.0007	0.0010
TG(49:1-15:0_34:1)	0.0019	0.0015	0.0018	0.0022	0.0019	0.0062	0.0038	0.0034	0.0026	0.0039
TG(49:1-17:0_32:1)	0.0010	0.0009	0.0011	0.0014	0.0011	0.0026	0.0018	0.0014	0.0011	0.0016
TG(50:0-18:0_32:0)	0.0130	0.0118	0.0131	0.0108	0.0107	0.0176	0.0115	0.0109	0.0075	0.0119
TG(50:1-14:0_36:1)	0.0020	0.0016	0.0019	0.0022	0.0020	0.0040	0.0027	0.0024	0.0017	0.0026
TG(50:1-18:1_32:0)	0.0371	0.0319	0.0375	0.0398	0.0363	0.0964	0.0678	0.0611	0.0456	0.0645
TG(50:2-18:0_32:2)	0.0010	0.0008	0.0011	0.0014	0.0013	0.0024	0.0015	0.0014	0.0010	0.0014
TG(50:2-18:1_32:1)	0.0207	0.0172	0.0213	0.0274	0.0262	0.0895	0.0598	0.0516	0.0398	0.0554
TG(50:2-18:2_32:0)	0.0069	0.0057	0.0071	0.0084	0.0072	0.0156	0.0100	0.0084	0.0056	0.0086
TG(50:3-14:1_36:2)	0.0001	0.0001	0.0001	0.0001	0.0002	0.0008	0.0005	0.0004	0.0003	0.0004
TG(50:3-18:1_32:2)	0.0021	0.0019	0.0023	0.0034	0.0032	0.0120	0.0075	0.0063	0.0048	0.0070
TG(50:3-18:2_32:1)	0.0034	0.0028	0.0037	0.0052	0.0049	0.0124	0.0077	0.0064	0.0045	0.0070
TG(50:4-14:0_36:4)	0.0003	0.0002	0.0003	0.0004	0.0004	0.0006	0.0004	0.0003	0.0002	0.0003
TG(50:4-18:2_32:2)	0.0004	0.0003	0.0004	0.0005	0.0005	0.0014	0.0008	0.0007	0.0005	0.0008
TG(51:0-18:0_33:0)	0.0013	0.0011	0.0012	0.0011	0.0010	0.0015	0.0009	0.0008	0.0005	0.0010
TG(51:1-18:1_33:0)	0.0059	0.0053	0.0058	0.0043	0.0053	0.0101	0.0073	0.0069	0.0050	0.0067
TG(51:2-15:0_36:2)	0.0013	0.0010	0.0013	0.0016	0.0013	0.0048	0.0030	0.0026	0.0019	0.0029
TG(51:2-16:0_35:2)	0.0061	0.0048	0.0061	0.0078	0.0070	0.0211	0.0131	0.0110	0.0085	0.0123
TG(51:2-16:1_35:1)	0.0025	0.0020	0.0025	0.0033	0.0030	0.0076	0.0047	0.0039	0.0031	0.0044
TG(52:1-18:1_34:0)	0.0355	0.0302	0.0338	0.0355	0.0313	0.0608	0.0403	0.0359	0.0259	0.0388
TG(52:2-16:0_36:2)	0.0517	0.0440	0.0509	0.0577	0.0553	0.1318	0.0935	0.0866	0.0637	0.0877
TG(52:3-16:1_36:2)	0.0129	0.0106	0.0130	0.0166	0.0160	0.0518	0.0341	0.0298	0.0223	0.0320
TG(52:3-18:2_34:1)	0.0197	0.0168	0.0199	0.0248	0.0239	0.0524	0.0349	0.0292	0.0219	0.0308
TG(52:4-16:0_36:4)	0.0046	0.0038	0.0045	0.0064	0.0049	0.0076	0.0053	0.0033	0.0029	0.0037
TG(52:4-16:1_36:3)	0.0051	0.0042	0.0051	0.0070	0.0072	0.0141	0.0091	0.0073	0.0052	0.0080
TG(52:4-20:4_32:0)	0.0094	0.0079	0.0095	0.0120	0.0117	0.0124	0.0078	0.0062	0.0048	0.0077
TG(52:5-20:4_32:1)	0.0038	0.0032	0.0041	0.0056	0.0055	0.0082	0.0049	0.0038	0.0030	0.0047
TG(53:2-17:0_36:2)	0.0041	0.0037	0.0039	0.0035	0.0042	0.0062	0.0041	0.0041	0.0030	0.0038
TG(54:1-18:1_36:0)	0.0070	0.0062	0.0067	0.0065	0.0055	0.0049	0.0029	0.0041	0.0018	0.0059
TG(54:2-18:0_36:2)	0.0319	0.0272	0.0303	0.0345	0.0306	0.0491	0.0320	0.0279	0.0204	0.0300
TG(54:2-20:2_34:0)	0.0016	0.0013	0.0016	0.0017	0.0014	0.0023	0.0015	0.0013	0.0010	0.0016
TG(54:3-18:1_36:2)	0.0694	0.0660	0.0684	0.0663	0.0777	0.1601	0.1218	0.1236	0.0860	0.1061
TG(54:3-20:3_34:0)	0.0001	0.0001	0.0001	0.0001	0.0001	0.0004	0.0002	0.0002	0.0002	0.0002
TG(54:4-18:0_36:4)	0.0025	0.0018	0.0021	0.0027	0.0025	0.0026	0.0020	0.0014	0.0010	0.0016
TG(54:4-18:2_36:2)	0.0112	0.0094	0.0109	0.0132	0.0136	0.0309	0.0194	0.0169	0.0125	0.0174
TG(54:4-20:4_34:0)	0.0080	0.0063	0.0077	0.0093	0.0083	0.0097	0.0060	0.0050	0.0038	0.0061
TG(54:5-18:1_36:4)	0.0041	0.0036	0.0039	0.0056	0.0053	0.0096	0.0062	0.0053	0.0036	0.0052
TG(54:5-20:4_34:1)	0.0171	0.0132	0.0172	0.0211	0.0213	0.0303	0.0192	0.0158	0.0118	0.0187
TG(54:5-22:5_32:0)	0.0067	0.0054	0.0066	0.0081	0.0076	0.0101	0.0065	0.0053	0.0042	0.0064
TG(54:6-18:2_36:4)	0.0006	0.0006	0.0006	0.0008	0.0009	0.0010	0.0006	0.0007	0.0004	0.0006
TG(54:6-18:3_36:3)	0.0003	0.0003	0.0003	0.0004	0.0004	0.0005	0.0004	0.0003	0.0002	0.0003
TG(54:6-20:4_34:2)	0.0039	0.0035	0.0045	0.0061	0.0060	0.0088	0.0048	0.0040	0.0031	0.0050
TG(54:6-22:6_32:0)	0.0056	0.0046	0.0057	0.0077	0.0070	0.0159	0.0101	0.0084	0.0065	0.0097
TG(56:6-20:4_36:2)	0.0117	0.0093	0.0119	0.0150	0.0142	0.0230	0.0144	0.0116	0.0092	0.0133
TG(56:6-20:5_36:1)	0.0019	0.0014	0.0019	0.0024	0.0022	0.0030	0.0019	0.0015	0.0012	0.0017
TG(56:6-22:5_34:1)	0.0151	0.0116	0.0148	0.0180	0.0184	0.0298	0.0198	0.0162	0.0129	0.0186
TG(56:6-22:6_34:0)	0.0048	0.0038	0.0047	0.0061	0.0051	0.0093	0.0059	0.0049	0.0040	0.0058
TG(56:8-20:4_36:4)	0.0015	0.0012	0.0016	0.0022	0.0021	0.0017	0.0009	0.0008	0.0006	0.0010
TG(58:8-22:6_36:2)	0.0085	0.0067	0.0084	0.0117	0.0109	0.0298	0.0201	0.0164	0.0129	0.0183
MS_METABOLITE_DATA_END
#METABOLITES
METABOLITES_START
metabolite_name	pubchem_id	inchi_key	kegg_id	other_id	other_id_type	ri	ri_type	moverz_quant	
TG(48:0-16:0_32:0)						11.7			
TG(48:1-16:1_32:0)						11.1			
TG(48:1-18:1_30:0)						11			
TG(48:2-14:1_34:1)						10.3			
TG(48:2-16:0_32:2)						10.4			
TG(48:2-18:1_30:1)						10.3			
TG(48:2-18:2_30:0)						10.4			
TG(48:3-14:0_34:3)						9.67			
TG(48:3-16:1_32:2)						9.66			
TG(49:1-14:0_35:1)						11.3			
TG(49:1-15:0_34:1)						11.3			
TG(49:1-17:0_32:1)						11.3			
TG(50:0-18:0_32:0)						12.3			
TG(50:1-14:0_36:1)						11.7			
TG(50:1-18:1_32:0)						11.7			
TG(50:2-18:0_32:2)						11.1			
TG(50:2-18:1_32:1)						11			
TG(50:2-18:2_32:0)						11.1			
TG(50:3-14:1_36:2)						10.3			
TG(50:3-18:1_32:2)						10.4			
TG(50:3-18:2_32:1)						10.4			
TG(50:4-14:0_36:4)						9.75			
TG(50:4-18:2_32:2)						9.71			
TG(51:0-18:0_33:0)						12.6			
TG(51:1-18:1_33:0)						12			
TG(51:2-15:0_36:2)						11.3			
TG(51:2-16:0_35:2)						11.3			
TG(51:2-16:1_35:1)						11.3			
TG(52:1-18:1_34:0)						12.3			
TG(52:2-16:0_36:2)						11.6			
TG(52:3-16:1_36:2)						11			
TG(52:3-18:2_34:1)						11.1			
TG(52:4-16:0_36:4)						10.5			
TG(52:4-16:1_36:3)						10.4			
TG(52:4-20:4_32:0)						10.9			
TG(52:5-20:4_32:1)						10.1			
TG(53:2-17:0_36:2)						12			
TG(54:1-18:1_36:0)						12.9			
TG(54:2-18:0_36:2)						12.3			
TG(54:2-20:2_34:0)						12.4			
TG(54:3-18:1_36:2)						11.6			
TG(54:3-20:3_34:0)						11.4			
TG(54:4-18:0_36:4)						11.2			
TG(54:4-18:2_36:2)						11.1			
TG(54:4-20:4_34:0)						11.6			
TG(54:5-18:1_36:4)						10.5			
TG(54:5-20:4_34:1)						10.9			
TG(54:5-22:5_32:0)						10.8			
TG(54:6-18:2_36:4)						9.83			
TG(54:6-18:3_36:3)						10			
TG(54:6-20:4_34:2)						10.2			
TG(54:6-22:6_32:0)						10.6			
TG(56:6-20:4_36:2)						10.8			
TG(56:6-20:5_36:1)						11			
TG(56:6-22:5_34:1)						10.8			
TG(56:6-22:6_34:0)						11.3			
TG(56:8-20:4_36:4)						10			
TG(58:8-22:6_36:2)						10.5			
METABOLITES_END
#END