#METABOLOMICS WORKBENCH Madjihounoum_20221203_055157 DATATRACK_ID:3622 STUDY_ID:ST002434 ANALYSIS_ID:AN003964 PROJECT_ID:PR001567 VERSION 1 CREATED_ON January 10, 2023, 8:43 am #PROJECT PR:PROJECT_TITLE Multiomics study of CHCHD10S59L-related disease reveals energy metabolism PR:PROJECT_TITLE downregulation: OXPHOS and β-oxidation deficiencies associated with lipids PR:PROJECT_TITLE alterations PR:PROJECT_SUMMARY Mutations in the coiled-coil-helix-coiled-coil-helix domain containing 10 PR:PROJECT_SUMMARY (CHCHD10) gene have been associated with a large clinical spectrum including PR:PROJECT_SUMMARY myopathy, cardiomyopathy and amyotrophic lateral sclerosis (ALS). Herein, we PR:PROJECT_SUMMARY analyzed the metabolic changes induced by the p.S59L CHCHD10 mutation to PR:PROJECT_SUMMARY identify new therapeutic opportunities. Using metabolomic, lipidomic and PR:PROJECT_SUMMARY proteomic analysis we observed a strong alteration of metabolism in plasma and PR:PROJECT_SUMMARY heart of Chchd10S59L/+ mice compared to their wild type littermates at PR:PROJECT_SUMMARY pre-symptomatic and symptomatic stages. In plasma, levels of phospholipids were PR:PROJECT_SUMMARY decreased while those of carnitine derivatives and most of amino acids were PR:PROJECT_SUMMARY increased. The cardiac tissue from Chchd10S59L/+ mice showed a decreased PR:PROJECT_SUMMARY Oxidative Phosphorylation (OXPHOS) and β-oxidation proteins levels as well as PR:PROJECT_SUMMARY tricarboxylic acid cycle (TCA) intermediates and carnitine pathway metabolism. PR:PROJECT_SUMMARY In parallel, lipidomics analysis reveals a drastic change in the lipidome, PR:PROJECT_SUMMARY including triglyceride, cardiolipin and phospholipids. Consistent with this PR:PROJECT_SUMMARY energetic deficiency in cardiac tissue, we show that L-acetylcarnitine PR:PROJECT_SUMMARY supplementation improves the mitochondrial network length in IPS-derived PR:PROJECT_SUMMARY cardiomyocytes from a patient carrying the CHCHD10S59L/+ mutation. These data PR:PROJECT_SUMMARY indicate that a bioenergetic intermediate such as L-acetylcarnitine may restore PR:PROJECT_SUMMARY mitochondrial function in CHCHD10-related disease, due to the reduction in PR:PROJECT_SUMMARY energy deficit that could be compensated by carnitine metabolic pathways. PR:INSTITUTE INSERM PR:DEPARTMENT C3M PR:LABORATORY RICCI PR:LAST_NAME Madji Hounoum PR:FIRST_NAME Blandine PR:ADDRESS 151 Route Saint Antoine de Genistière 06204 Nice PR:EMAIL madjihounoum@yahoo.fr PR:PHONE +33 (0)4 89 06 43 01 #STUDY ST:STUDY_TITLE Metabolomics analysis of heart from CHCHD10S59L/+ KI mice ST:STUDY_SUMMARY Mutations in the coiled-coil-helix-coiled-coil-helix domain containing 10 ST:STUDY_SUMMARY (CHCHD10) gene have been associated with a large clinical spectrum including ST:STUDY_SUMMARY myopathy, cardiomyopathy and amyotrophic lateral sclerosis (ALS). Herein, we ST:STUDY_SUMMARY analyzed the metabolic changes induced by the p.S59L CHCHD10 mutation to ST:STUDY_SUMMARY identify new therapeutic opportunities. Using metabolomic, lipidomic and ST:STUDY_SUMMARY proteomic analysis we observed a strong alteration of metabolism in plasma and ST:STUDY_SUMMARY heart of Chchd10S59L/+ mice compared to their wild type littermates at ST:STUDY_SUMMARY pre-symptomatic and symptomatic stages. In plasma, levels of phospholipids were ST:STUDY_SUMMARY decreased while those of carnitine derivatives and most of amino acids were ST:STUDY_SUMMARY increased. The cardiac tissue from Chchd10S59L/+ mice showed a decreased ST:STUDY_SUMMARY Oxidative Phosphorylation (OXPHOS) and β-oxidation proteins levels as well as ST:STUDY_SUMMARY tricarboxylic acid cycle (TCA) intermediates and carnitine pathway metabolism. ST:STUDY_SUMMARY In parallel, lipidomics analysis reveals a drastic change in the lipidome, ST:STUDY_SUMMARY including triglyceride, cardiolipin and phospholipids. Consistent with this ST:STUDY_SUMMARY energetic deficiency in cardiac tissue, we show that L-acetylcarnitine ST:STUDY_SUMMARY supplementation improves the mitochondrial network length in IPS-derived ST:STUDY_SUMMARY cardiomyocytes from a patient carrying the CHCHD10S59L/+ mutation. These data ST:STUDY_SUMMARY indicate that a bioenergetic intermediate such as L-acetylcarnitine may restore ST:STUDY_SUMMARY mitochondrial function in CHCHD10-related disease, due to the reduction in ST:STUDY_SUMMARY energy deficit that could be compensated by carnitine metabolic pathways. ST:INSTITUTE INSERM ST:LAST_NAME Madji Hounoum ST:FIRST_NAME Blandine ST:ADDRESS 151 Route Saint Antoine de Genistière 06204 NIce ST:EMAIL madjihounoum@yahoo.fr ST:PHONE +33 (0)4 89 06 43 01 #SUBJECT SU:SUBJECT_TYPE Mammal SU:SUBJECT_SPECIES Mus musculus SU:TAXONOMY_ID 10090 #FACTORS #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data SUBJECT_SAMPLE_FACTORS - Heart_1 Genotype:Wild-type | Gender:Male | Stage:Symptomatic RAW_FILE_NAME=HILIC_POS_Heart_1.mzXML SUBJECT_SAMPLE_FACTORS - Heart_2 Genotype:Wild-type | Gender:Male | Stage:Symptomatic RAW_FILE_NAME=HILIC_POS_Heart_2.mzXML SUBJECT_SAMPLE_FACTORS - Heart_3 Genotype:Wild-type | Gender:Male | Stage:Symptomatic RAW_FILE_NAME=HILIC_POS_Heart_3.mzXML SUBJECT_SAMPLE_FACTORS - Heart_4 Genotype:Wild-type | Gender:Male | Stage:Symptomatic RAW_FILE_NAME=HILIC_POS_Heart_4.mzXML SUBJECT_SAMPLE_FACTORS - Heart_5 Genotype:Wild-type | Gender:Male | Stage:Symptomatic RAW_FILE_NAME=HILIC_POS_Heart_5.mzXML SUBJECT_SAMPLE_FACTORS - Heart_6 Genotype:Wild-type | Gender:Male | Stage:Symptomatic RAW_FILE_NAME=HILIC_POS_Heart_6.mzXML SUBJECT_SAMPLE_FACTORS - Heart_7 Genotype:CHCHD10S59L/+ | Gender:Male | Stage:Symptomatic RAW_FILE_NAME=HILIC_POS_Heart_7.mzXML SUBJECT_SAMPLE_FACTORS - Heart_8 Genotype:CHCHD10S59L/+ | Gender:Male | Stage:Symptomatic RAW_FILE_NAME=HILIC_POS_Heart_8.mzXML SUBJECT_SAMPLE_FACTORS - Heart_9 Genotype:CHCHD10S59L/+ | Gender:Male | Stage:Symptomatic RAW_FILE_NAME=HILIC_POS_Heart_9.mzXML SUBJECT_SAMPLE_FACTORS - Heart_10 Genotype:CHCHD10S59L/+ | Gender:Male | Stage:Symptomatic RAW_FILE_NAME=HILIC_POS_Heart_10.mzXML SUBJECT_SAMPLE_FACTORS - Heart_11 Genotype:CHCHD10S59L/+ | Gender:Male | Stage:Symptomatic RAW_FILE_NAME=HILIC_POS_Heart_11.mzXML SUBJECT_SAMPLE_FACTORS - Heart_12 Genotype:CHCHD10S59L/+ | Gender:Male | Stage:Symptomatic RAW_FILE_NAME=HILIC_POS_Heart_12.mzXML SUBJECT_SAMPLE_FACTORS - Heart_13 Genotype:Wild-type | Gender:female | Stage:Symptomatic RAW_FILE_NAME=HILIC_POS_Heart_13.mzXML SUBJECT_SAMPLE_FACTORS - Heart_14 Genotype:Wild-type | Gender:female | Stage:Symptomatic RAW_FILE_NAME=HILIC_POS_Heart_14.mzXML SUBJECT_SAMPLE_FACTORS - Heart_15 Genotype:Wild-type | Gender:Male | Stage:Symptomatic RAW_FILE_NAME=HILIC_POS_Heart_15.mzXML SUBJECT_SAMPLE_FACTORS - Heart_16 Genotype:CHCHD10S59L/+ | Gender:female | Stage:Symptomatic RAW_FILE_NAME=HILIC_POS_Heart_16.mzXML SUBJECT_SAMPLE_FACTORS - Heart_17 Genotype:CHCHD10S59L/+ | Gender:female | Stage:Symptomatic RAW_FILE_NAME=HILIC_POS_Heart_17.mzXML SUBJECT_SAMPLE_FACTORS - Heart_18 Genotype:CHCHD10S59L/+ | Gender:Male | Stage:Symptomatic RAW_FILE_NAME=HILIC_POS_Heart_18.mzXML SUBJECT_SAMPLE_FACTORS - Heart_19 Genotype:Wild-type | Gender:female | Stage:Symptomatic RAW_FILE_NAME=HILIC_POS_Heart_19.mzXML SUBJECT_SAMPLE_FACTORS - Heart_20 Genotype:Wild-type | Gender:female | Stage:Symptomatic RAW_FILE_NAME=HILIC_POS_Heart_20.mzXML SUBJECT_SAMPLE_FACTORS - Heart_21 Genotype:Wild-type | Gender:Male | Stage:Symptomatic RAW_FILE_NAME=HILIC_POS_Heart_21.mzXML SUBJECT_SAMPLE_FACTORS - Heart_22 Genotype:CHCHD10S59L/+ | Gender:female | Stage:Symptomatic RAW_FILE_NAME=HILIC_POS_Heart_22.mzXML SUBJECT_SAMPLE_FACTORS - Heart_23 Genotype:CHCHD10S59L/+ | Gender:female | Stage:Symptomatic RAW_FILE_NAME=HILIC_POS_Heart_23.mzXML SUBJECT_SAMPLE_FACTORS - Heart_24 Genotype:CHCHD10S59L/+ | Gender:Male | Stage:Symptomatic RAW_FILE_NAME=HILIC_POS_Heart_24.mzXML SUBJECT_SAMPLE_FACTORS - Heart_QC1 Genotype:QC | Gender:QC | Stage:QC RAW_FILE_NAME=HILIC_POS_Heart_QC1.mzXML SUBJECT_SAMPLE_FACTORS - Heart_QC2 Genotype:QC | Gender:QC | Stage:QC RAW_FILE_NAME=HILIC_POS_Heart_QC2.mzXML SUBJECT_SAMPLE_FACTORS - Heart_QC3 Genotype:QC | Gender:QC | Stage:QC RAW_FILE_NAME=HILIC_POS_Heart_QC3.mzXML SUBJECT_SAMPLE_FACTORS - Heart_QC4 Genotype:QC | Gender:QC | Stage:QC RAW_FILE_NAME=HILIC_POS_Heart_QC4.mzXML SUBJECT_SAMPLE_FACTORS - Heart_QC5 Genotype:QC | Gender:QC | Stage:QC RAW_FILE_NAME=HILIC_POS_Heart_QC5.mzXML #COLLECTION CO:COLLECTION_SUMMARY Cardiac tissue samples were immediately flash frozen in liquid nitrogen and CO:COLLECTION_SUMMARY stored at -80 °C until further analyses CO:SAMPLE_TYPE Cardiac tissue CO:STORAGE_CONDITIONS -80℃ #TREATMENT TR:TREATMENT_SUMMARY NA #SAMPLEPREP SP:SAMPLEPREP_SUMMARY Tissues were ground to powder by using liquid nitrogen cooled mortar and pestle. SP:SAMPLEPREP_SUMMARY 11.1 mg of powder tissue were extracted with 1 mL cold acetonitrile/water (1:1, SP:SAMPLEPREP_SUMMARY v/v). The suspension was vortexed, incubated at 4°C for 1 h, centrifuged at 16 SP:SAMPLEPREP_SUMMARY 000 x g for 10 mins at 4°C and then 450 µL of the supernatant was dried. For SP:SAMPLEPREP_SUMMARY plasma metabolites extraction, 20 µL were precipitated with methanol and SP:SAMPLEPREP_SUMMARY centrifuged at 16 000 x g for 10 mins at 4°C, and the supernatant was dried. SP:SAMPLEPREP_SUMMARY All dried metabolites extract from tissues were reconstituted in 100 µL of SP:SAMPLEPREP_SUMMARY either methanol/water (1: 9, v/v) or ACN/water (9: 1, v/v) depending on the SP:SAMPLEPREP_SUMMARY LC-column used and 10 µL was injected into LC-HRMS system SP:PROCESSING_STORAGE_CONDITIONS 4℃ SP:EXTRACT_STORAGE -80℃ #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE HILIC CH:INSTRUMENT_NAME Thermo Dionex Ultimate 3000 CH:COLUMN_NAME Thermo Accucore HILIC (100 x 2.1mm, 2.6um) CH:SOLVENT_A Water CH:SOLVENT_B Methanol CH:FLOW_GRADIENT 20 minutes CH:FLOW_RATE 0.4 mL/min #ANALYSIS AN:ANALYSIS_TYPE MS #MS MS:INSTRUMENT_NAME Thermo Q Exactive Plus Orbitrap MS:INSTRUMENT_TYPE Orbitrap MS:MS_TYPE ESI MS:ION_MODE POSITIVE MS:MS_COMMENTS During the full-scan acquisition, which ranged from 58 to 870 m/z, the MS:MS_COMMENTS instrument operated at 70,000 resolution (m/z = 200).A targeted analysis was MS:MS_COMMENTS applied on the samples, based on a library of standard compounds (Mass MS:MS_COMMENTS Spectroscopy Metabolite Library (MSML®) of standards, IROA Technologies™). MS:MS_COMMENTS The following criteria were followed to identify the metabolites: (1) retention MS:MS_COMMENTS time of the detected metabolite within ± 20 s of the standard reference, (2) MS:MS_COMMENTS exact measured of molecular mass of the metabolite within a range of 10 ppm MS:MS_COMMENTS around the known molecular mass of the reference compound, and (3) MS:MS_COMMENTS correspondence between isotopic ratios of the metabolite and the standard MS:MS_COMMENTS reference. The signal value was calculated using Xcalibur® software (Thermo MS:MS_COMMENTS Fisher Scientific, San Jose, CA) by integrating the chromatographic peak area MS:MS_COMMENTS corresponding to the selected metabolite. #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS relative units MS_METABOLITE_DATA_START Samples Heart_1 Heart_10 Heart_11 Heart_12 Heart_13 Heart_14 Heart_15 Heart_16 Heart_17 Heart_18 Heart_19 Heart_2 Heart_20 Heart_21 Heart_22 Heart_23 Heart_24 Heart_3 Heart_4 Heart_5 Heart_6 Heart_7 Heart_8 Heart_9 Heart_QC1 Heart_QC2 Heart_QC3 Heart_QC4 Heart_QC5 Factors Genotype:Wild-type | Gender:Male | Stage:Symptomatic Genotype:CHCHD10S59L/+ | Gender:Male | Stage:Symptomatic Genotype:CHCHD10S59L/+ | Gender:Male | Stage:Symptomatic Genotype:CHCHD10S59L/+ | Gender:Male | Stage:Symptomatic Genotype:Wild-type | Gender:female | Stage:Symptomatic Genotype:Wild-type | Gender:female | Stage:Symptomatic Genotype:Wild-type | Gender:Male | Stage:Symptomatic Genotype:CHCHD10S59L/+ | Gender:female | Stage:Symptomatic Genotype:CHCHD10S59L/+ | Gender:female | Stage:Symptomatic Genotype:CHCHD10S59L/+ | Gender:Male | Stage:Symptomatic Genotype:Wild-type | Gender:female | Stage:Symptomatic Genotype:Wild-type | Gender:Male | Stage:Symptomatic Genotype:Wild-type | Gender:female | Stage:Symptomatic Genotype:Wild-type | Gender:Male | Stage:Symptomatic Genotype:CHCHD10S59L/+ | Gender:female | Stage:Symptomatic Genotype:CHCHD10S59L/+ | Gender:female | Stage:Symptomatic Genotype:CHCHD10S59L/+ | Gender:Male | Stage:Symptomatic Genotype:Wild-type | Gender:Male | Stage:Symptomatic Genotype:Wild-type | Gender:Male | Stage:Symptomatic Genotype:Wild-type | Gender:Male | Stage:Symptomatic Genotype:Wild-type | Gender:Male | Stage:Symptomatic Genotype:CHCHD10S59L/+ | Gender:Male | Stage:Symptomatic Genotype:CHCHD10S59L/+ | Gender:Male | Stage:Symptomatic Genotype:CHCHD10S59L/+ | Gender:Male | Stage:Symptomatic Genotype:QC | Gender:QC | Stage:QC Genotype:QC | Gender:QC | Stage:QC Genotype:QC | Gender:QC | Stage:QC Genotype:QC | Gender:QC | Stage:QC Genotype:QC | Gender:QC | Stage:QC CHOLESTERYL_ACETATE 205E+06 275E+06 148E+06 167E+06 488E+06 184E+06 785E+05 439E+06 475E+06 378E+06 106E+06 159E+06 476E+06 725E+06 673E+06 182E+06 371E+06 103E+06 449E+05 823E+04 660E+05 358E+06 793E+06 300E+06 140E-05 812E-06 100E-05 101E-05 104E-05 GAMMA-LINOLENIC_ACID 380E+07 210E+06 373E+06 228E+06 522E+07 142E+07 136E+07 735E+06 931E+06 566E+06 772E+06 211E+07 278E+07 306E+07 394E+06 652E+06 500E+06 594E+06 654E+06 742E+05 311E+06 184E+07 450E+07 990E+06 462E-05 470E-05 542E-05 525E-05 549E-05 ALPHA-TOCOPHEROL 133E+06 214E+06 639E+06 218E+06 298E+06 175E+06 565E+06 282E+06 428E+06 103E+07 454E+06 147E+06 128E+06 275E+06 246E+06 462E+06 106E+06 359E+06 197E+06 105E+06 108E+06 143E+06 919E+05 277E+06 129E-05 192E-05 117E-05 172E-05 788E-06 PANTOTHENIC_ACID 652E+07 480E+07 276E+07 113E+08 310E+08 344E+08 185E+08 107E+08 139E+08 550E+07 227E+08 102E+08 294E+08 990E+07 117E+08 393E+08 128E+08 656E+07 194E+08 201E+07 131E+08 905E+07 110E+08 107E+08 500E-04 601E-04 609E-04 568E-04 609E-04 URACIL 446E+06 470E+06 813E+06 317E+07 135E+07 142E+07 112E+07 172E+07 378E+07 160E+07 108E+07 936E+06 116E+07 111E+07 115E+07 602E+07 245E+07 413E+06 115E+07 145E+06 894E+06 157E+07 348E+07 118E+07 550E-05 159E-05 594E-05 663E-05 657E-05 NICOTINAMIDE 195E+09 101E+09 170E+09 326E+09 444E+09 296E+09 313E+09 211E+09 282E+09 170E+09 207E+09 264E+09 284E+09 311E+09 150E+09 357E+09 279E+09 199E+09 335E+09 610E+08 348E+09 265E+09 300E+09 234E+09 109E-02 995E-03 112E-02 104E-02 107E-02 5-METHYLTHIOADENOSINE 290E+06 394E+06 136E+07 132E+07 101E+07 887E+06 735E+06 121E+07 972E+06 159E+07 948E+06 859E+06 653E+06 856E+06 834E+06 202E+07 170E+07 109E+07 780E+06 225E+06 770E+06 305E+07 129E+07 116E+07 543E-05 200E-05 408E-05 455E-05 480E-05 3-UREIDOPROPIONATE 145E+06 573E+05 120E+06 962E+05 101E+07 174E+06 733E+05 542E+05 518E+05 781E+05 499E+06 189E+06 295E+06 331E+06 687E+05 777E+05 168E+06 236E+06 200E+06 417E+05 107E+06 131E+06 588E+05 661E+05 309E-06 628E-06 891E-06 747E-06 820E-06 GLYCOLALDEHYDE_DIMER 584E+05 732E+05 134E+06 158E+06 135E+06 919E+05 732E+05 114E+06 142E+06 125E+06 775E+05 106E+06 741E+05 815E+05 148E+06 244E+06 186E+06 844E+05 122E+06 210E+05 945E+05 118E+06 109E+06 124E+06 578E-06 440E-06 573E-06 409E-06 452E-06 GLUCURONIC_ACID 250E+05 182E+05 237E+05 341E+05 164E+05 218E+05 219E+05 126E+05 115E+05 220E+05 175E+05 790E+04 236E+05 204E+05 123E+05 214E+05 104E+05 872E+04 264E+05 383E+03 147E+05 287E+05 213E+05 167E+05 780E-07 724E-07 672E-07 761E-07 745E-07 GLUCURONOLACTONE 250E+05 182E+05 237E+05 341E+05 164E+05 218E+05 219E+05 126E+05 115E+05 220E+05 175E+05 790E+04 236E+05 204E+05 123E+05 214E+05 104E+05 872E+04 264E+05 383E+03 147E+05 287E+05 213E+05 167E+05 780E-07 724E-07 672E-07 761E-07 745E-07 UROCANATE 973E+06 849E+06 186E+07 196E+07 270E+07 468E+07 373E+07 209E+07 525E+07 244E+07 771E+07 137E+07 241E+07 172E+07 434E+07 240E+07 953E+07 834E+06 193E+07 125E+06 121E+07 324E+07 265E+07 248E+07 116E-04 127E-04 139E-04 128E-04 134E-04 RIBOFLAVIN 906E+05 295E+05 865E+05 201E+06 284E+06 142E+06 112E+06 129E+06 215E+06 120E+06 104E+06 105E+06 114E+06 101E+06 893E+05 293E+06 231E+06 571E+05 205E+06 213E+05 141E+06 229E+06 227E+06 145E+06 678E-06 424E-06 540E-06 564E-06 549E-06 1-METHYLADENOSINE 133E+05 270E+04 169E+05 281E+05 252E+05 977E+04 584E+04 249E+05 324E+05 341E+05 115E+05 974E+04 160E+05 153E+05 211E+05 420E+05 277E+05 103E+05 640E+04 000E+00 112E+05 396E+05 292E+05 146E+05 680E-07 577E-07 643E-07 720E-07 657E-07 HYPOXANTHINE 689E+08 100E+09 795E+08 207E+09 165E+09 123E+09 144E+09 129E+09 202E+09 758E+08 796E+08 802E+08 100E+09 117E+09 970E+08 231E+09 218E+09 104E+09 110E+09 489E+08 189E+09 266E+09 920E+08 151E+09 706E-03 616E-03 576E-03 515E-03 548E-03 INOSINE 588E+07 151E+08 859E+07 324E+08 115E+08 958E+07 124E+08 160E+08 313E+08 814E+07 582E+07 577E+07 735E+07 797E+07 125E+08 342E+08 357E+08 609E+07 101E+08 610E+07 181E+08 347E+08 138E+08 251E+08 117E-03 778E-04 647E-04 596E-04 620E-04 ADENOSINE 390E+08 367E+07 159E+09 234E+09 261E+09 249E+09 128E+09 226E+09 267E+09 260E+09 211E+09 196E+09 184E+09 231E+09 187E+09 365E+09 719E+08 117E+09 183E+09 385E+07 716E+08 422E+08 377E+09 506E+06 236E-05 611E-03 789E-03 718E-03 757E-03 3-AMINO-4-HYDROXYBENZOIC_ACID 389E+06 323E+06 280E+06 135E+06 630E+06 323E+06 372E+06 280E+06 454E+06 320E+06 307E+06 430E+06 320E+06 270E+06 313E+06 647E+06 692E+06 483E+06 312E+06 417E+05 593E+06 542E+06 541E+06 712E+06 332E-05 943E-06 160E-05 162E-05 160E-05 GUANINE 124E+07 940E+07 165E+08 352E+08 110E+08 860E+07 597E+07 258E+08 330E+08 209E+08 749E+07 679E+07 553E+07 720E+07 203E+08 379E+08 254E+08 466E+07 630E+07 734E+06 767E+07 298E+08 334E+08 146E+08 682E-04 678E-04 716E-04 677E-04 694E-04 GUANOSINE 918E+06 684E+07 120E+08 296E+08 806E+07 679E+07 468E+07 201E+08 282E+08 148E+08 606E+07 502E+07 436E+07 584E+07 160E+08 322E+08 206E+08 386E+07 488E+07 540E+06 637E+07 190E+08 289E+08 118E+08 548E-04 543E-04 571E-04 543E-04 557E-04 ADENINE 740E+07 527E+06 661E+07 499E+08 346E+08 418E+08 189E+08 118E+08 345E+08 144E+08 228E+08 230E+08 274E+08 240E+08 494E+07 247E+08 684E+07 115E+08 372E+08 352E+07 215E+08 511E+07 296E+08 682E+06 318E-05 719E-04 845E-04 796E-04 822E-04 5-OXO-L-PROLINE 546E+06 148E+06 289E+06 308E+06 904E+06 334E+06 368E+06 386E+06 416E+06 565E+06 448E+06 551E+06 770E+06 886E+06 422E+06 368E+06 548E+06 645E+06 354E+06 390E+05 304E+06 321E+05 544E+06 417E+06 195E-05 124E-05 210E-05 192E-05 235E-05 CYTOSINE 742E+07 848E+07 104E+08 277E+08 164E+08 141E+08 136E+08 189E+08 304E+08 164E+08 116E+08 136E+08 155E+08 150E+08 129E+08 360E+08 244E+08 770E+07 139E+08 218E+07 131E+08 239E+08 289E+08 143E+08 666E-04 665E-04 671E-04 685E-04 709E-04 CYTIDINE 242E+07 282E+07 346E+07 953E+07 523E+07 488E+07 456E+07 626E+07 108E+08 564E+07 384E+07 436E+07 525E+07 499E+07 433E+07 121E+08 783E+07 257E+07 464E+07 697E+06 454E+07 788E+07 101E+08 415E+07 194E-04 213E-04 223E-04 235E-04 240E-04 DEOXYCYTIDINE 564E+05 819E+05 122E+06 223E+06 206E+06 134E+06 131E+06 187E+06 268E+06 118E+06 129E+06 155E+06 150E+06 157E+06 142E+06 410E+06 417E+06 854E+05 127E+06 131E+05 130E+06 292E+06 210E+06 306E+06 143E-05 584E-06 614E-06 707E-06 676E-06 PYRIDOXINE 388E+06 714E+05 303E+06 236E+06 121E+07 217E+06 233E+06 267E+06 392E+06 431E+06 247E+06 467E+06 678E+06 967E+06 280E+06 184E+06 211E+06 304E+06 222E+06 293E+05 747E+05 584E+06 379E+06 128E+06 595E-06 111E-05 114E-05 166E-05 209E-05 SEROTONINE 195E+03 346E+07 226E+07 644E+07 628E+05 183E+07 228E+06 990E+06 357E+07 777E+06 139E+07 198E+06 109E+04 242E+04 449E+06 330E+07 205E+08 103E+06 209E+07 327E+06 620E+07 153E+07 688E+06 233E+07 109E-04 817E-05 968E-05 101E-04 963E-05 CREATININE 127E+08 759E+07 234E+08 300E+08 467E+08 234E+08 162E+08 207E+08 255E+08 200E+08 196E+08 203E+08 279E+08 333E+08 146E+08 264E+08 273E+08 127E+08 215E+08 303E+07 261E+08 199E+08 267E+08 136E+08 634E-04 783E-04 801E-04 872E-04 871E-04 TYRAMINE 212E+06 317E+06 326E+06 326E+06 291E+06 327E+06 295E+06 340E+06 325E+06 296E+06 299E+06 203E+06 293E+06 288E+06 290E+06 307E+06 299E+06 191E+06 288E+06 268E+05 313E+06 382E+06 384E+06 333E+06 156E-05 119E-05 101E-05 114E-05 114E-05 TAURINE 232E+09 158E+09 165E+09 291E+09 328E+09 302E+09 288E+09 239E+09 287E+09 186E+09 248E+09 261E+09 337E+09 285E+09 200E+09 304E+09 354E+09 186E+09 345E+09 835E+08 325E+09 290E+09 303E+09 242E+09 113E-02 926E-03 112E-02 104E-02 116E-02 METHYL-GUANIDINE 563E+06 128E+06 296E+06 341E+06 126E+07 498E+06 377E+06 231E+06 314E+06 266E+06 394E+06 432E+06 736E+06 759E+06 228E+06 222E+06 437E+06 200E+06 420E+06 634E+05 369E+06 250E+06 337E+06 181E+06 843E-06 140E-05 142E-05 159E-05 155E-05 4-GUANIDINOBUTANOATE 578E+06 304E+06 773E+06 938E+06 124E+07 620E+06 565E+06 817E+06 680E+06 657E+06 113E+07 499E+06 734E+06 727E+06 114E+07 148E+06 105E+07 573E+06 584E+06 707E+05 544E+06 326E+06 366E+06 360E+06 168E-05 221E-05 231E-05 274E-05 263E-05 LAUROYLCARNITINE 106E+07 335E+05 758E+05 139E+06 871E+06 292E+07 782E+06 273E+06 574E+06 183E+06 139E+07 115E+07 235E+07 170E+07 112E+06 654E+06 211E+06 294E+06 102E+07 136E+06 304E+06 285E+06 870E+06 196E+06 913E-06 194E-05 237E-05 324E-05 297E-05 L-TRYPTOPHAN 120E+07 179E+07 813E+07 102E+08 104E+08 687E+07 258E+07 813E+07 798E+07 647E+07 630E+07 454E+07 307E+07 389E+07 479E+07 851E+07 870E+07 609E+07 602E+07 879E+06 556E+07 821E+07 873E+07 457E+07 213E-04 187E-04 238E-04 250E-04 243E-04 L-PHENYLALANINE 209E+07 280E+07 495E+07 927E+07 627E+07 348E+07 229E+07 729E+07 117E+08 555E+07 335E+07 424E+07 353E+07 483E+07 836E+07 176E+08 136E+08 283E+07 470E+07 915E+06 326E+07 651E+07 770E+07 703E+07 328E-04 276E-04 247E-04 227E-04 242E-04 NORLEUCINE 323E+05 127E+05 602E+05 477E+05 357E+05 265E+05 613E+05 106E+05 180E+05 150E+06 205E+05 120E+06 264E+05 190E+06 418E+04 133E+05 746E+05 376E+05 987E+05 110E+05 685E+05 895E+05 145E+06 518E+05 242E-06 218E-06 211E-06 230E-06 217E-06 2-AMINO-2-METHYLPROPANOATE 380E+05 445E+05 215E+05 609E+04 760E+05 867E+05 206E+05 620E+05 428E+05 186E+05 963E+04 197E+04 142E+05 774E+04 767E+04 587E+04 355E+04 402E+04 117E+05 807E+04 118E+05 117E+06 250E+05 193E+05 902E-07 210E-06 151E-06 996E-07 109E-06 L-TYROSINE 254E+07 267E+07 619E+07 102E+08 777E+07 498E+07 418E+07 559E+07 689E+07 501E+07 363E+07 551E+07 298E+07 551E+07 370E+07 569E+07 711E+07 315E+07 626E+07 107E+07 293E+07 500E+07 751E+07 324E+07 151E-04 207E-04 214E-04 194E-04 202E-04 LEUCINE 631E+07 661E+07 128E+08 211E+08 155E+08 871E+07 652E+07 130E+08 184E+08 130E+08 968E+07 102E+08 846E+07 736E+07 111E+08 210E+08 251E+08 767E+07 102E+08 229E+07 838E+07 157E+08 156E+08 143E+08 668E-04 416E-04 500E-04 467E-04 489E-04 ETHANOLAMINE 475E+05 103E+06 178E+06 250E+06 159E+06 145E+06 120E+06 310E+06 344E+06 163E+06 156E+06 174E+06 102E+06 161E+06 168E+06 355E+06 390E+06 878E+05 999E+05 151E+05 138E+06 191E+06 200E+06 192E+06 893E-06 601E-06 719E-06 653E-06 721E-06 L-ISOLEUCINE 102E+07 870E+06 188E+07 305E+07 268E+07 129E+07 996E+06 222E+07 297E+07 198E+07 148E+07 181E+07 123E+07 114E+07 173E+07 297E+07 487E+07 170E+07 149E+07 266E+06 172E+07 295E+07 264E+07 236E+07 110E-04 110E-04 829E-05 750E-05 803E-05 L-METHIONINE 648E+05 429E+06 126E+07 222E+07 606E+06 745E+06 471E+06 142E+07 241E+07 114E+07 689E+06 722E+06 176E+06 218E+06 888E+06 170E+07 234E+07 504E+06 979E+06 145E+06 647E+06 938E+06 147E+07 751E+06 350E-05 686E-05 418E-05 341E-05 405E-05 3-AMINOISOBUTANOATE 335E+06 622E+05 796E+05 147E+06 217E+06 202E+06 269E+06 933E+05 193E+06 846E+05 257E+06 326E+06 395E+06 408E+06 102E+06 117E+06 148E+06 170E+06 407E+06 986E+05 395E+06 146E+06 151E+06 112E+06 524E-06 151E-05 935E-06 773E-06 896E-06 DIETHANOLAMINE 287E+06 421E+06 424E+06 444E+06 313E+06 189E+06 200E+06 233E+06 331E+06 683E+06 368E+06 137E+06 279E+06 254E+06 685E+06 199E+06 434E+06 224E+06 159E+06 335E+05 385E+06 368E+06 367E+06 289E+06 135E-05 140E-05 130E-05 124E-05 130E-05 L-VALINE 376E+07 540E+07 101E+08 131E+08 105E+08 561E+07 433E+07 105E+08 122E+08 862E+07 610E+07 614E+07 534E+07 546E+07 836E+07 878E+07 170E+08 612E+07 543E+07 122E+07 550E+07 110E+08 102E+08 956E+07 446E-04 337E-04 310E-04 314E-04 318E-04 L-THREONINE 237E+07 538E+07 586E+07 992E+07 438E+07 310E+07 285E+07 790E+07 112E+08 494E+07 334E+07 310E+07 371E+07 350E+07 795E+07 841E+07 106E+08 283E+07 386E+07 395E+06 268E+07 611E+07 620E+07 552E+07 257E-04 192E-04 209E-04 175E-04 216E-04 TRIMETHYLAMINE 241E+06 291E+06 811E+06 143E+07 108E+07 889E+06 789E+06 141E+07 178E+07 907E+06 866E+06 773E+06 708E+06 105E+07 837E+06 137E+07 117E+07 572E+06 608E+06 562E+05 725E+06 105E+07 938E+06 588E+06 274E-05 329E-05 290E-05 323E-05 308E-05 GUANIDINOACETATE 371E+06 144E+06 502E+06 759E+06 649E+06 513E+06 426E+06 723E+06 688E+06 731E+06 761E+06 401E+06 541E+06 607E+06 239E+06 332E+06 815E+06 364E+06 450E+06 376E+05 497E+06 839E+06 878E+06 491E+06 229E-05 192E-05 191E-05 179E-05 201E-05 L-ALANINE 969E+07 100E+08 115E+08 197E+08 137E+08 133E+08 132E+08 154E+08 216E+08 116E+08 111E+08 933E+07 149E+08 117E+08 136E+08 137E+08 222E+08 719E+07 132E+08 212E+07 135E+08 164E+08 137E+08 114E+08 533E-04 561E-04 527E-04 439E-04 525E-04 L-SERINE 765E+06 157E+07 224E+07 323E+07 101E+07 116E+07 878E+06 269E+07 302E+07 246E+07 120E+07 761E+06 102E+07 172E+07 205E+07 186E+07 278E+07 793E+06 107E+07 116E+06 836E+06 214E+07 233E+07 146E+07 679E-05 661E-05 619E-05 485E-05 660E-05 4-HYDROXY-L-PROLINE 173E+07 189E+07 375E+07 714E+07 237E+07 244E+07 195E+07 442E+07 391E+07 463E+07 197E+07 200E+07 218E+07 214E+07 207E+07 944E+06 570E+07 165E+07 352E+07 417E+06 248E+07 362E+07 486E+07 833E+06 388E-05 119E-04 107E-04 100E-04 117E-04 L-PROLINE 109E+08 238E+08 605E+08 961E+08 343E+08 190E+08 157E+08 518E+08 725E+08 505E+08 177E+08 200E+08 180E+08 238E+08 410E+08 549E+08 911E+08 132E+08 195E+08 311E+07 134E+08 453E+08 502E+08 326E+08 152E-03 147E-03 129E-03 136E-03 145E-03 L-GLUTAMIC_ACID 285E+08 224E+08 187E+08 289E+08 228E+08 306E+08 254E+08 221E+08 240E+08 220E+08 295E+08 177E+08 393E+08 252E+08 166E+08 144E+08 168E+08 934E+07 303E+08 281E+07 211E+08 275E+08 289E+08 127E+08 593E-04 833E-04 868E-04 673E-04 903E-04 GLYCINE 270E+06 693E+06 895E+06 124E+07 492E+06 432E+06 439E+06 121E+07 133E+07 104E+07 501E+06 392E+06 389E+06 445E+06 102E+07 115E+07 144E+07 350E+06 440E+06 607E+05 465E+06 118E+07 124E+07 114E+07 530E-05 353E-05 310E-05 258E-05 305E-05 2,6-DIAMINOHEPTANEDIOATE 231E+06 348E+05 272E+05 580E+05 884E+05 155E+06 186E+06 267E+05 358E+05 298E+05 110E+06 921E+05 218E+06 128E+06 149E+05 227E+05 143E+06 332E+05 169E+06 184E+05 103E+06 902E+05 119E+06 124E+06 579E-06 361E-06 332E-06 337E-06 407E-06 SARCOSINE 977E+05 237E+06 400E+06 683E+06 363E+06 233E+06 221E+06 479E+06 425E+06 334E+06 198E+06 172E+06 260E+06 168E+06 330E+06 172E+06 974E+06 997E+05 151E+06 249E+05 141E+06 340E+06 343E+06 106E+06 495E-06 102E-05 881E-06 762E-06 132E-05 5-AMINOPENTANOATE 204E+06 105E+06 176E+07 845E+07 536E+06 541E+06 741E+06 684E+06 567E+06 374E+07 335E+06 343E+06 477E+06 402E+06 102E+06 123E+07 459E+06 302E+06 241E+06 600E+05 853E+06 118E+07 120E+07 405E+06 189E-05 356E-05 341E-05 379E-05 386E-05 L-ASPARAGINE 780E+06 156E+07 263E+07 344E+07 107E+07 131E+07 992E+06 274E+07 261E+07 229E+07 938E+06 895E+06 117E+07 213E+07 181E+07 225E+07 324E+07 681E+06 129E+07 111E+06 921E+06 217E+07 249E+07 237E+07 111E-04 779E-05 647E-05 547E-05 709E-05 CREATINE 261E+09 272E+09 248E+09 377E+09 424E+09 350E+09 370E+09 334E+09 311E+09 274E+09 304E+09 268E+09 388E+09 393E+09 296E+09 328E+09 368E+09 231E+09 333E+09 796E+08 391E+09 420E+09 345E+09 322E+09 150E-02 142E-02 123E-02 126E-02 127E-02 BETA-ALANINE 330E+07 342E+07 313E+07 462E+07 519E+07 431E+07 453E+07 418E+07 394E+07 338E+07 366E+07 332E+07 471E+07 476E+07 370E+07 409E+07 469E+07 288E+07 409E+07 101E+07 479E+07 512E+07 421E+07 400E+07 186E-04 178E-04 154E-04 153E-04 155E-04 L-GLUTAMINE 538E+08 268E+08 322E+08 495E+08 545E+08 586E+08 579E+08 397E+08 385E+08 363E+08 498E+08 446E+08 667E+08 718E+08 291E+08 445E+08 433E+08 376E+08 591E+08 162E+08 528E+08 416E+08 465E+08 418E+08 195E-03 208E-03 190E-03 180E-03 196E-03 O-ACETYL-CARNITINE 191E+09 364E+08 616E+08 124E+09 288E+09 317E+09 269E+09 102E+09 127E+09 159E+09 380E+09 323E+09 342E+09 297E+09 748E+08 906E+08 150E+09 233E+09 486E+09 679E+08 270E+09 194E+09 216E+09 141E+09 659E-03 932E-03 800E-03 926E-03 915E-03 DEOXYCARNITINE 593E+07 377E+07 777E+07 167E+08 206E+08 168E+08 143E+08 946E+07 131E+08 111E+08 135E+08 950E+07 184E+08 271E+08 938E+07 101E+08 147E+08 779E+07 150E+08 177E+07 167E+08 152E+08 158E+08 924E+07 431E-04 500E-04 433E-04 521E-04 463E-04 L-CARNITINE 305E+09 115E+09 210E+09 463E+09 892E+09 579E+09 754E+09 378E+09 349E+09 240E+09 448E+09 369E+09 787E+09 740E+09 213E+09 302E+09 328E+09 266E+09 579E+09 701E+08 713E+09 344E+09 273E+09 201E+09 939E-03 169E-02 162E-02 173E-02 151E-02 BETAINE 380E+08 465E+08 136E+09 301E+09 143E+09 829E+08 752E+08 214E+09 217E+09 223E+09 114E+09 821E+08 827E+08 154E+09 118E+09 176E+09 233E+09 739E+08 838E+08 131E+08 763E+08 192E+09 241E+09 961E+08 448E-03 500E-03 472E-03 527E-03 446E-03 N-ACETYL-LYSINE 768E+05 414E+05 740E+05 123E+06 210E+06 114E+06 982E+05 993E+05 932E+05 868E+05 977E+05 955E+05 160E+06 205E+06 523E+05 738E+05 127E+06 696E+05 919E+05 759E+04 938E+05 167E+06 145E+06 995E+05 464E-06 393E-06 381E-06 431E-06 370E-06 CITRULLINE 416E+07 255E+07 325E+07 523E+07 539E+07 520E+07 464E+07 479E+07 475E+07 367E+07 389E+07 322E+07 593E+07 605E+07 460E+07 276E+07 506E+07 247E+07 466E+07 385E+06 448E+07 341E+07 460E+07 246E+07 115E-04 169E-04 161E-04 158E-04 161E-04 TRIGONELLINE 140E+07 399E+07 658E+07 223E+08 179E+07 325E+07 215E+07 637E+07 182E+08 113E+08 152E+07 192E+07 194E+07 251E+07 479E+07 914E+07 114E+08 103E+07 229E+07 240E+06 245E+07 159E+08 189E+08 637E+07 297E-04 238E-04 213E-04 234E-04 214E-04 HISTAMINE 123E+07 313E+07 938E+07 108E+08 424E+07 427E+07 398E+07 229E+08 864E+07 516E+07 121E+08 871E+07 178E+07 129E+07 113E+08 586E+07 287E+08 399E+07 575E+07 515E+06 480E+07 603E+07 264E+07 235E+08 110E-03 314E-04 293E-04 308E-04 290E-04 L-ARGININE 191E+07 207E+07 325E+07 238E+07 417E+07 239E+07 243E+07 445E+07 296E+07 357E+07 282E+07 324E+07 231E+07 342E+07 363E+07 267E+07 306E+07 504E+07 262E+07 349E+06 453E+07 577E+07 525E+07 536E+07 250E-04 145E-04 133E-04 127E-04 128E-04 Thiamine 553E+05 597E+05 283E+06 668E+06 355E+06 292E+06 343E+06 462E+06 744E+06 279E+06 185E+06 217E+06 168E+06 207E+06 178E+06 922E+06 344E+06 155E+06 229E+06 200E+05 291E+06 289E+06 398E+06 165E+06 768E-06 113E-05 105E-05 120E-05 117E-05 L-HISTIDINE 693E+07 749E+07 994E+07 151E+08 118E+08 115E+08 108E+08 140E+08 155E+08 884E+07 929E+07 967E+07 105E+08 120E+08 112E+08 189E+08 212E+08 880E+07 148E+08 185E+07 140E+08 178E+08 160E+08 175E+08 816E-04 512E-04 497E-04 482E-04 474E-04 L-ORNITHINE 329E+05 937E+05 339E+06 152E+06 723E+05 290E+05 334E+05 862E+05 884E+05 932E+05 114E+06 446E+05 714E+05 864E+05 102E+06 328E+05 282E+06 709E+05 437E+05 631E+04 308E+05 118E+06 715E+05 161E+06 750E-06 491E-06 442E-06 432E-06 422E-06 L-LYSINE 330E+06 503E+06 113E+07 719E+06 749E+06 529E+06 513E+06 911E+06 751E+06 763E+06 626E+06 649E+06 509E+06 659E+06 704E+06 729E+06 108E+07 728E+06 563E+06 714E+05 690E+06 966E+06 102E+07 106E+07 495E-05 305E-05 292E-05 260E-05 255E-05 CARNOSINE 134E+06 326E+07 120E+07 229E+07 161E+06 153E+06 176E+06 158E+07 166E+07 103E+07 111E+06 120E+06 449E+05 129E+06 173E+07 160E+07 276E+07 833E+05 176E+06 246E+05 174E+06 232E+07 261E+07 383E+07 178E-04 436E-05 409E-05 401E-05 397E-05 GLYCERO-3-PHOSPHOCHOLINE 106E+08 109E+08 192E+08 320E+08 168E+08 171E+08 858E+07 297E+08 281E+08 177E+08 243E+08 158E+08 283E+08 184E+08 132E+08 167E+08 363E+08 620E+07 179E+08 156E+07 166E+08 428E+08 354E+08 179E+08 833E-04 723E-04 667E-04 731E-04 736E-04 L-ANSERINE 139E+06 345E+06 309E+06 382E+06 567E+06 489E+06 324E+06 439E+06 437E+06 353E+06 215E+06 134E+06 944E+05 165E+06 261E+06 253E+06 583E+06 119E+06 158E+06 233E+05 202E+06 490E+06 423E+06 573E+06 267E-05 131E-05 117E-05 111E-05 120E-05 N,N,N-TRIMETHYL-LYSINE 103E+06 142E+06 481E+06 191E+06 479E+06 150E+06 222E+06 318E+06 222E+06 485E+06 264E+06 209E+06 268E+06 474E+06 406E+06 203E+06 331E+06 635E+06 113E+06 276E+05 351E+06 113E+07 341E+06 592E+06 276E-05 123E-05 116E-05 134E-05 107E-05 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name RT(min) CHOLESTERYL_ACETATE 1.1387737 GAMMA-LINOLENIC_ACID 1.1699719 ALPHA-TOCOPHEROL 1.1970469 PANTOTHENIC_ACID 1.541622 URACIL 1.60402 NICOTINAMIDE 1.6896409 5-METHYLTHIOADENOSINE 1.6940989 3-UREIDOPROPIONATE 1.769868 GLYCOLALDEHYDE_DIMER 1.832268 GLUCURONIC_ACID 1.8902097 GLUCURONOLACTONE 1.8902097 UROCANATE 1.912493 RIBOFLAVIN 1.9407618 1-METHYLADENOSINE 1.9496761 HYPOXANTHINE 2.0715283 INOSINE 2.09827 ADENOSINE 2.1250117 3-AMINO-4-HYDROXYBENZOIC_ACID 2.1887911 GUANINE 2.3642182 GUANOSINE 2.3642182 ADENINE 2.4816723 5-OXO-L-PROLINE 2.7462389 CYTOSINE 2.9083098 CYTIDINE 2.9083098 DEOXYCYTIDINE 3.064306 PYRIDOXINE 3.1550831 SEROTONINE 3.3885123 CREATININE 3.4642832 TYRAMINE 3.5327103 TAURINE 3.6678374 METHYL-GUANIDINE 4.4483669 4-GUANIDINOBUTANOATE 4.8807003 LAUROYLCARNITINE 4.9982441 L-TRYPTOPHAN 5.1202338 L-PHENYLALANINE 5.5720525 NORLEUCINE 5.5898798 2-AMINO-2-METHYLPROPANOATE 5.7520965 L-TYROSINE 5.8635237 LEUCINE 5.95433 ETHANOLAMINE 6.1387778 L-ISOLEUCINE 6.1655194 L-METHIONINE 6.225032 3-AMINOISOBUTANOATE 6.3515155 DIETHANOLAMINE 6.4915279 L-VALINE 6.9098488 L-THREONINE 7.7330074 TRIMETHYLAMINE 7.7879658 GUANIDINOACETATE 7.9929911 L-ALANINE 8.0750474 L-SERINE 8.10624529999999 4-HYDROXY-L-PROLINE 8.3349537 L-PROLINE 8.36615349999999 L-GLUTAMIC_ACID 8.43891419999999 GLYCINE 8.501314 2,6-DIAMINOHEPTANEDIOATE 8.5742767 SARCOSINE 8.6233036 5-AMINOPENTANOATE 8.7097789 L-ASPARAGINE 8.74543489999999 CREATINE 8.8746914 BETA-ALANINE 8.8791474 L-GLUTAMINE 8.9549165 O-ACETYL-CARNITINE 8.9727454 DEOXYCARNITINE 9.012858 L-CARNITINE 9.5035271 BETAINE 9.6297376 N-ACETYL-LYSINE 9.7740126 CITRULLINE 9.97903769999999 TRIGONELLINE 10.130575 HISTAMINE 12.605487 L-ARGININE 12.705266 Thiamine 12.830064 L-HISTIDINE 13.076955 L-ORNITHINE 13.22577 L-LYSINE 13.612581 CARNOSINE 14.833754 GLYCERO-3-PHOSPHOCHOLINE 15.169791 L-ANSERINE 17.216992 N,N,N-TRIMETHYL-LYSINE 17.824482 METABOLITES_END #END