#METABOLOMICS WORKBENCH Madjihounoum_20221203_055157 DATATRACK_ID:3622 STUDY_ID:ST002434 ANALYSIS_ID:AN003965 PROJECT_ID:PR001567 VERSION 1 CREATED_ON January 10, 2023, 8:43 am #PROJECT PR:PROJECT_TITLE Multiomics study of CHCHD10S59L-related disease reveals energy metabolism PR:PROJECT_TITLE downregulation: OXPHOS and β-oxidation deficiencies associated with lipids PR:PROJECT_TITLE alterations PR:PROJECT_SUMMARY Mutations in the coiled-coil-helix-coiled-coil-helix domain containing 10 PR:PROJECT_SUMMARY (CHCHD10) gene have been associated with a large clinical spectrum including PR:PROJECT_SUMMARY myopathy, cardiomyopathy and amyotrophic lateral sclerosis (ALS). Herein, we PR:PROJECT_SUMMARY analyzed the metabolic changes induced by the p.S59L CHCHD10 mutation to PR:PROJECT_SUMMARY identify new therapeutic opportunities. Using metabolomic, lipidomic and PR:PROJECT_SUMMARY proteomic analysis we observed a strong alteration of metabolism in plasma and PR:PROJECT_SUMMARY heart of Chchd10S59L/+ mice compared to their wild type littermates at PR:PROJECT_SUMMARY pre-symptomatic and symptomatic stages. In plasma, levels of phospholipids were PR:PROJECT_SUMMARY decreased while those of carnitine derivatives and most of amino acids were PR:PROJECT_SUMMARY increased. The cardiac tissue from Chchd10S59L/+ mice showed a decreased PR:PROJECT_SUMMARY Oxidative Phosphorylation (OXPHOS) and β-oxidation proteins levels as well as PR:PROJECT_SUMMARY tricarboxylic acid cycle (TCA) intermediates and carnitine pathway metabolism. PR:PROJECT_SUMMARY In parallel, lipidomics analysis reveals a drastic change in the lipidome, PR:PROJECT_SUMMARY including triglyceride, cardiolipin and phospholipids. Consistent with this PR:PROJECT_SUMMARY energetic deficiency in cardiac tissue, we show that L-acetylcarnitine PR:PROJECT_SUMMARY supplementation improves the mitochondrial network length in IPS-derived PR:PROJECT_SUMMARY cardiomyocytes from a patient carrying the CHCHD10S59L/+ mutation. These data PR:PROJECT_SUMMARY indicate that a bioenergetic intermediate such as L-acetylcarnitine may restore PR:PROJECT_SUMMARY mitochondrial function in CHCHD10-related disease, due to the reduction in PR:PROJECT_SUMMARY energy deficit that could be compensated by carnitine metabolic pathways. PR:INSTITUTE INSERM PR:DEPARTMENT C3M PR:LABORATORY RICCI PR:LAST_NAME Madji Hounoum PR:FIRST_NAME Blandine PR:ADDRESS 151 Route Saint Antoine de Genistière 06204 Nice PR:EMAIL madjihounoum@yahoo.fr PR:PHONE +33 (0)4 89 06 43 01 #STUDY ST:STUDY_TITLE Metabolomics analysis of heart from CHCHD10S59L/+ KI mice ST:STUDY_SUMMARY Mutations in the coiled-coil-helix-coiled-coil-helix domain containing 10 ST:STUDY_SUMMARY (CHCHD10) gene have been associated with a large clinical spectrum including ST:STUDY_SUMMARY myopathy, cardiomyopathy and amyotrophic lateral sclerosis (ALS). Herein, we ST:STUDY_SUMMARY analyzed the metabolic changes induced by the p.S59L CHCHD10 mutation to ST:STUDY_SUMMARY identify new therapeutic opportunities. Using metabolomic, lipidomic and ST:STUDY_SUMMARY proteomic analysis we observed a strong alteration of metabolism in plasma and ST:STUDY_SUMMARY heart of Chchd10S59L/+ mice compared to their wild type littermates at ST:STUDY_SUMMARY pre-symptomatic and symptomatic stages. In plasma, levels of phospholipids were ST:STUDY_SUMMARY decreased while those of carnitine derivatives and most of amino acids were ST:STUDY_SUMMARY increased. The cardiac tissue from Chchd10S59L/+ mice showed a decreased ST:STUDY_SUMMARY Oxidative Phosphorylation (OXPHOS) and β-oxidation proteins levels as well as ST:STUDY_SUMMARY tricarboxylic acid cycle (TCA) intermediates and carnitine pathway metabolism. ST:STUDY_SUMMARY In parallel, lipidomics analysis reveals a drastic change in the lipidome, ST:STUDY_SUMMARY including triglyceride, cardiolipin and phospholipids. Consistent with this ST:STUDY_SUMMARY energetic deficiency in cardiac tissue, we show that L-acetylcarnitine ST:STUDY_SUMMARY supplementation improves the mitochondrial network length in IPS-derived ST:STUDY_SUMMARY cardiomyocytes from a patient carrying the CHCHD10S59L/+ mutation. These data ST:STUDY_SUMMARY indicate that a bioenergetic intermediate such as L-acetylcarnitine may restore ST:STUDY_SUMMARY mitochondrial function in CHCHD10-related disease, due to the reduction in ST:STUDY_SUMMARY energy deficit that could be compensated by carnitine metabolic pathways. ST:INSTITUTE INSERM ST:LAST_NAME Madji Hounoum ST:FIRST_NAME Blandine ST:ADDRESS 151 Route Saint Antoine de Genistière 06204 NIce ST:EMAIL madjihounoum@yahoo.fr ST:PHONE +33 (0)4 89 06 43 01 #SUBJECT SU:SUBJECT_TYPE Mammal SU:SUBJECT_SPECIES Mus musculus SU:TAXONOMY_ID 10090 #FACTORS #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data SUBJECT_SAMPLE_FACTORS - Heart_1 Genotype:Wild-type | Gender:Male | Stage:Symptomatic RAW_FILE_NAME=HILIC_POS_Heart_1.mzXML SUBJECT_SAMPLE_FACTORS - Heart_2 Genotype:Wild-type | Gender:Male | Stage:Symptomatic RAW_FILE_NAME=HILIC_POS_Heart_2.mzXML SUBJECT_SAMPLE_FACTORS - Heart_3 Genotype:Wild-type | Gender:Male | Stage:Symptomatic RAW_FILE_NAME=HILIC_POS_Heart_3.mzXML SUBJECT_SAMPLE_FACTORS - Heart_4 Genotype:Wild-type | Gender:Male | Stage:Symptomatic RAW_FILE_NAME=HILIC_POS_Heart_4.mzXML SUBJECT_SAMPLE_FACTORS - Heart_5 Genotype:Wild-type | Gender:Male | Stage:Symptomatic RAW_FILE_NAME=HILIC_POS_Heart_5.mzXML SUBJECT_SAMPLE_FACTORS - Heart_6 Genotype:Wild-type | Gender:Male | Stage:Symptomatic RAW_FILE_NAME=HILIC_POS_Heart_6.mzXML SUBJECT_SAMPLE_FACTORS - Heart_7 Genotype:CHCHD10S59L/+ | Gender:Male | Stage:Symptomatic RAW_FILE_NAME=HILIC_POS_Heart_7.mzXML SUBJECT_SAMPLE_FACTORS - Heart_8 Genotype:CHCHD10S59L/+ | Gender:Male | Stage:Symptomatic RAW_FILE_NAME=HILIC_POS_Heart_8.mzXML SUBJECT_SAMPLE_FACTORS - Heart_9 Genotype:CHCHD10S59L/+ | Gender:Male | Stage:Symptomatic RAW_FILE_NAME=HILIC_POS_Heart_9.mzXML SUBJECT_SAMPLE_FACTORS - Heart_10 Genotype:CHCHD10S59L/+ | Gender:Male | Stage:Symptomatic RAW_FILE_NAME=HILIC_POS_Heart_10.mzXML SUBJECT_SAMPLE_FACTORS - Heart_11 Genotype:CHCHD10S59L/+ | Gender:Male | Stage:Symptomatic RAW_FILE_NAME=HILIC_POS_Heart_11.mzXML SUBJECT_SAMPLE_FACTORS - Heart_12 Genotype:CHCHD10S59L/+ | Gender:Male | Stage:Symptomatic RAW_FILE_NAME=HILIC_POS_Heart_12.mzXML SUBJECT_SAMPLE_FACTORS - Heart_13 Genotype:Wild-type | Gender:female | Stage:Symptomatic RAW_FILE_NAME=HILIC_POS_Heart_13.mzXML SUBJECT_SAMPLE_FACTORS - Heart_14 Genotype:Wild-type | Gender:female | Stage:Symptomatic RAW_FILE_NAME=HILIC_POS_Heart_14.mzXML SUBJECT_SAMPLE_FACTORS - Heart_15 Genotype:Wild-type | Gender:Male | Stage:Symptomatic RAW_FILE_NAME=HILIC_POS_Heart_15.mzXML SUBJECT_SAMPLE_FACTORS - Heart_16 Genotype:CHCHD10S59L/+ | Gender:female | Stage:Symptomatic RAW_FILE_NAME=HILIC_POS_Heart_16.mzXML SUBJECT_SAMPLE_FACTORS - Heart_17 Genotype:CHCHD10S59L/+ | Gender:female | Stage:Symptomatic RAW_FILE_NAME=HILIC_POS_Heart_17.mzXML SUBJECT_SAMPLE_FACTORS - Heart_18 Genotype:CHCHD10S59L/+ | Gender:Male | Stage:Symptomatic RAW_FILE_NAME=HILIC_POS_Heart_18.mzXML SUBJECT_SAMPLE_FACTORS - Heart_19 Genotype:Wild-type | Gender:female | Stage:Symptomatic RAW_FILE_NAME=HILIC_POS_Heart_19.mzXML SUBJECT_SAMPLE_FACTORS - Heart_20 Genotype:Wild-type | Gender:female | Stage:Symptomatic RAW_FILE_NAME=HILIC_POS_Heart_20.mzXML SUBJECT_SAMPLE_FACTORS - Heart_21 Genotype:Wild-type | Gender:Male | Stage:Symptomatic RAW_FILE_NAME=HILIC_POS_Heart_21.mzXML SUBJECT_SAMPLE_FACTORS - Heart_22 Genotype:CHCHD10S59L/+ | Gender:female | Stage:Symptomatic RAW_FILE_NAME=HILIC_POS_Heart_22.mzXML SUBJECT_SAMPLE_FACTORS - Heart_23 Genotype:CHCHD10S59L/+ | Gender:female | Stage:Symptomatic RAW_FILE_NAME=HILIC_POS_Heart_23.mzXML SUBJECT_SAMPLE_FACTORS - Heart_24 Genotype:CHCHD10S59L/+ | Gender:Male | Stage:Symptomatic RAW_FILE_NAME=HILIC_POS_Heart_24.mzXML SUBJECT_SAMPLE_FACTORS - Heart_QC1 Genotype:QC | Gender:QC | Stage:QC RAW_FILE_NAME=HILIC_POS_Heart_QC1.mzXML SUBJECT_SAMPLE_FACTORS - Heart_QC2 Genotype:QC | Gender:QC | Stage:QC RAW_FILE_NAME=HILIC_POS_Heart_QC2.mzXML SUBJECT_SAMPLE_FACTORS - Heart_QC3 Genotype:QC | Gender:QC | Stage:QC RAW_FILE_NAME=HILIC_POS_Heart_QC3.mzXML SUBJECT_SAMPLE_FACTORS - Heart_QC4 Genotype:QC | Gender:QC | Stage:QC RAW_FILE_NAME=HILIC_POS_Heart_QC4.mzXML SUBJECT_SAMPLE_FACTORS - Heart_QC5 Genotype:QC | Gender:QC | Stage:QC RAW_FILE_NAME=HILIC_POS_Heart_QC5.mzXML #COLLECTION CO:COLLECTION_SUMMARY Cardiac tissue samples were immediately flash frozen in liquid nitrogen and CO:COLLECTION_SUMMARY stored at -80 °C until further analyses CO:SAMPLE_TYPE Cardiac tissue CO:STORAGE_CONDITIONS -80℃ #TREATMENT TR:TREATMENT_SUMMARY NA #SAMPLEPREP SP:SAMPLEPREP_SUMMARY Tissues were ground to powder by using liquid nitrogen cooled mortar and pestle. SP:SAMPLEPREP_SUMMARY 11.1 mg of powder tissue were extracted with 1 mL cold acetonitrile/water (1:1, SP:SAMPLEPREP_SUMMARY v/v). The suspension was vortexed, incubated at 4°C for 1 h, centrifuged at 16 SP:SAMPLEPREP_SUMMARY 000 x g for 10 mins at 4°C and then 450 µL of the supernatant was dried. For SP:SAMPLEPREP_SUMMARY plasma metabolites extraction, 20 µL were precipitated with methanol and SP:SAMPLEPREP_SUMMARY centrifuged at 16 000 x g for 10 mins at 4°C, and the supernatant was dried. SP:SAMPLEPREP_SUMMARY All dried metabolites extract from tissues were reconstituted in 100 µL of SP:SAMPLEPREP_SUMMARY either methanol/water (1: 9, v/v) or ACN/water (9: 1, v/v) depending on the SP:SAMPLEPREP_SUMMARY LC-column used and 10 µL was injected into LC-HRMS system SP:PROCESSING_STORAGE_CONDITIONS 4℃ SP:EXTRACT_STORAGE -80℃ #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE Reversed phase CH:INSTRUMENT_NAME Thermo Dionex Ultimate 3000 CH:COLUMN_NAME Phenomenex Kinetex C18 (150 x 2.1mm, 2.6 um) CH:SOLVENT_A Water CH:SOLVENT_B ACN CH:FLOW_GRADIENT 20 minutes CH:FLOW_RATE 0.4 mL/min #ANALYSIS AN:ANALYSIS_TYPE MS #MS MS:INSTRUMENT_NAME Thermo Q Exactive Plus Orbitrap MS:INSTRUMENT_TYPE Orbitrap MS:MS_TYPE ESI MS:ION_MODE POSITIVE MS:MS_COMMENTS During the full-scan acquisition, which ranged from 58 to 870 m/z, the MS:MS_COMMENTS instrument operated at 70,000 resolution (m/z = 200).A targeted analysis was MS:MS_COMMENTS applied on the samples, based on a library of standard compounds (Mass MS:MS_COMMENTS Spectroscopy Metabolite Library (MSML®) of standards, IROA Technologies™). MS:MS_COMMENTS The following criteria were followed to identify the metabolites: (1) retention MS:MS_COMMENTS time of the detected metabolite within ± 20 s of the standard reference, (2) MS:MS_COMMENTS exact measured of molecular mass of the metabolite within a range of 10 ppm MS:MS_COMMENTS around the known molecular mass of the reference compound, and (3) MS:MS_COMMENTS correspondence between isotopic ratios of the metabolite and the standard MS:MS_COMMENTS reference. The signal value was calculated using Xcalibur® software (Thermo MS:MS_COMMENTS Fisher Scientific, San Jose, CA) by integrating the chromatographic peak area MS:MS_COMMENTS corresponding to the selected metabolite. #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS relative units MS_METABOLITE_DATA_START Samples Heart_1 Heart_10 Heart_11 Heart_12 Heart_13 Heart_14 Heart_15 Heart_16 Heart_17 Heart_18 Heart_19 Heart_2 Heart_20 Heart_21 Heart_22 Heart_23 Heart_24 Heart_3 Heart_4 Heart_5 Heart_6 Heart_7 Heart_8 Heart_9 Heart_QC1 Heart_QC2 Heart_QC3 Heart_QC4 Heart_QC5 Factors Genotype:Wild-type | Gender:Male | Stage:Symptomatic Genotype:CHCHD10S59L/+ | Gender:Male | Stage:Symptomatic Genotype:CHCHD10S59L/+ | Gender:Male | Stage:Symptomatic Genotype:CHCHD10S59L/+ | Gender:Male | Stage:Symptomatic Genotype:Wild-type | Gender:female | Stage:Symptomatic Genotype:Wild-type | Gender:female | Stage:Symptomatic Genotype:Wild-type | Gender:Male | Stage:Symptomatic Genotype:CHCHD10S59L/+ | Gender:female | Stage:Symptomatic Genotype:CHCHD10S59L/+ | Gender:female | Stage:Symptomatic Genotype:CHCHD10S59L/+ | Gender:Male | Stage:Symptomatic Genotype:Wild-type | Gender:female | Stage:Symptomatic Genotype:Wild-type | Gender:Male | Stage:Symptomatic Genotype:Wild-type | Gender:female | Stage:Symptomatic Genotype:Wild-type | Gender:Male | Stage:Symptomatic Genotype:CHCHD10S59L/+ | Gender:female | Stage:Symptomatic Genotype:CHCHD10S59L/+ | Gender:female | Stage:Symptomatic Genotype:CHCHD10S59L/+ | Gender:Male | Stage:Symptomatic Genotype:Wild-type | Gender:Male | Stage:Symptomatic Genotype:Wild-type | Gender:Male | Stage:Symptomatic Genotype:Wild-type | Gender:Male | Stage:Symptomatic Genotype:Wild-type | Gender:Male | Stage:Symptomatic Genotype:CHCHD10S59L/+ | Gender:Male | Stage:Symptomatic Genotype:CHCHD10S59L/+ | Gender:Male | Stage:Symptomatic Genotype:CHCHD10S59L/+ | Gender:Male | Stage:Symptomatic Genotype:QC | Gender:QC | Stage:QC Genotype:QC | Gender:QC | Stage:QC Genotype:QC | Gender:QC | Stage:QC Genotype:QC | Gender:QC | Stage:QC Genotype:QC | Gender:QC | Stage:QC SPERMIDINE 271E+05 000E+00 294E+06 654E+05 451E+06 315E+04 804E+04 129E+06 336E+06 745E+06 182E+05 363E+05 306E+05 513E+06 368E+06 215E+06 106E+06 342E+06 119E+05 355E+05 104E+05 110E+06 247E+06 760E+06 258E+06 248E+06 219E+06 187E+06 188E+06 L-ORNITHINE 944E+05 315E+05 747E+06 405E+06 216E+06 667E+05 661E+05 161E+06 328E+06 277E+06 113E+06 128E+06 689E+05 123E+06 256E+06 914E+05 480E+06 207E+06 692E+05 126E+06 846E+05 118E+06 124E+06 151E+06 232E+06 226E+06 213E+06 226E+06 234E+06 L-LYSINE 219E+07 161E+05 379E+07 274E+07 424E+07 270E+07 189E+07 310E+07 411E+07 357E+07 188E+07 347E+07 282E+07 358E+07 282E+07 287E+07 307E+07 343E+07 188E+07 225E+07 270E+07 207E+07 370E+07 219E+07 298E+07 291E+07 289E+07 286E+07 292E+07 HISTAMINE 498E+06 000E+00 105E+07 723E+06 699E+06 238E+06 139E+06 238E+07 139E+07 871E+06 306E+06 170E+07 238E+06 246E+06 192E+07 566E+06 284E+07 691E+06 131E+06 119E+06 278E+06 326E+06 343E+06 254E+07 921E+06 892E+06 888E+06 885E+06 890E+06 3-METHYLHISTAMINE 109E+06 000E+00 831E+05 692E+05 241E+06 561E+05 740E+05 108E+06 105E+06 109E+06 791E+05 172E+06 944E+05 102E+06 863E+05 779E+05 207E+06 107E+06 250E+05 420E+05 139E+06 653E+05 595E+05 964E+05 116E+06 106E+06 106E+06 102E+06 100E+06 N,N,N-TRIMETHYL-LYSINE 112E+07 000E+00 264E+07 757E+06 278E+07 894E+06 971E+06 151E+07 171E+07 347E+07 907E+06 203E+07 180E+07 279E+07 272E+07 125E+07 132E+07 474E+07 585E+06 129E+07 199E+07 243E+07 215E+07 188E+07 201E+07 190E+07 186E+07 179E+07 185E+07 DIETHANOLAMINE 267E+05 298E+05 348E+05 140E+05 315E+05 147E+05 154E+05 255E+05 185E+05 205E+05 261E+05 103E+05 122E+05 193E+05 250E+05 159E+05 205E+05 258E+05 229E+05 988E+04 299E+05 252E+05 151E+05 130E+05 293E+05 260E+05 326E+05 249E+05 303E+05 CHOLINE 211E+05 000E+00 420E+05 435E+05 416E+05 323E+05 298E+05 498E+05 801E+05 508E+05 320E+05 426E+05 324E+05 421E+05 498E+05 522E+05 469E+05 405E+05 216E+05 180E+05 323E+05 301E+05 365E+05 214E+05 395E+05 360E+05 380E+05 325E+05 350E+05 L-ARGININE 464E+07 361E+05 436E+07 296E+07 757E+07 430E+07 346E+07 533E+07 531E+07 746E+07 328E+07 687E+07 470E+07 729E+07 543E+07 369E+07 339E+07 991E+07 342E+07 427E+07 694E+07 434E+07 643E+07 426E+07 526E+07 516E+07 508E+07 487E+07 495E+07 L-HISTIDINE 194E+07 338E+05 189E+07 249E+07 266E+07 172E+07 104E+07 221E+07 351E+07 264E+07 829E+06 247E+07 188E+07 243E+07 222E+07 331E+07 259E+07 168E+07 136E+07 121E+07 163E+07 130E+07 294E+07 211E+07 217E+07 213E+07 214E+07 204E+07 212E+07 3-Methylhistidine 428E+06 398E+03 914E+06 784E+06 729E+06 463E+06 404E+06 732E+06 619E+06 107E+07 229E+06 600E+06 418E+06 463E+06 309E+06 315E+06 770E+06 455E+06 413E+06 349E+06 416E+06 501E+06 114E+07 285E+06 595E+06 555E+06 552E+06 510E+06 529E+06 EPINEPHRINE 459E+06 000E+00 475E+06 349E+06 142E+07 493E+06 527E+06 528E+06 606E+06 678E+06 341E+06 835E+06 581E+06 979E+06 439E+06 381E+06 471E+06 919E+06 427E+06 589E+06 818E+06 284E+06 463E+06 297E+06 544E+06 506E+06 509E+06 491E+06 517E+06 4-AMINOBUTANOATE 146E+06 000E+00 389E+06 281E+06 268E+06 122E+06 994E+05 265E+06 345E+06 440E+06 203E+06 218E+06 104E+06 122E+06 215E+06 185E+07 310E+06 301E+06 126E+06 141E+06 197E+06 413E+06 475E+06 132E+07 356E+06 393E+06 358E+06 353E+06 355E+06 CARNOSINE 769E+05 000E+00 320E+06 637E+06 155E+06 651E+05 341E+05 379E+06 817E+06 493E+06 243E+05 107E+06 505E+05 112E+06 499E+06 493E+06 487E+06 108E+06 458E+05 621E+05 769E+05 222E+06 803E+06 417E+06 286E+06 269E+06 265E+06 248E+06 261E+06 5-HYDROXYLYSINE 136E+06 338E+03 691E+05 744E+05 295E+06 161E+06 177E+06 105E+06 108E+06 995E+05 813E+05 183E+06 217E+06 227E+06 676E+05 743E+05 779E+05 107E+06 157E+06 149E+06 211E+06 461E+05 715E+05 341E+05 125E+06 124E+06 125E+06 119E+06 121E+06 L-ANSERINE 456E+04 000E+00 411E+05 438E+05 202E+06 872E+05 250E+04 521E+05 103E+06 774E+05 410E+04 453E+05 233E+04 349E+05 383E+05 341E+05 610E+05 249E+05 193E+05 214E+05 224E+05 211E+05 619E+05 360E+05 504E+05 471E+05 442E+05 482E+05 457E+05 L-CARNITINE 494E+08 112E+05 282E+08 320E+08 916E+08 571E+08 635E+08 369E+08 416E+08 376E+08 327E+08 637E+08 703E+08 742E+08 251E+08 292E+08 295E+08 412E+08 555E+08 523E+08 703E+08 203E+08 284E+08 173E+08 446E+08 448E+08 444E+08 421E+08 436E+08 S-Adenosylmethionine 129E+06 000E+00 118E+06 137E+06 164E+06 135E+06 101E+06 135E+06 191E+06 147E+06 824E+05 172E+06 139E+06 149E+06 876E+05 158E+06 116E+06 115E+06 130E+06 148E+06 145E+06 100E+06 192E+06 859E+05 129E+06 139E+06 148E+06 131E+06 129E+06 GLYCINE 744E+06 359E+05 236E+07 251E+07 114E+07 985E+06 806E+06 298E+07 347E+07 316E+07 884E+06 128E+07 810E+06 955E+06 235E+07 299E+07 265E+07 995E+06 774E+06 710E+06 862E+06 185E+07 308E+07 246E+07 186E+07 184E+07 197E+07 188E+07 192E+07 L-SERINE 148E+07 305E+06 512E+07 535E+07 215E+07 200E+07 139E+07 518E+07 644E+07 614E+07 157E+07 223E+07 153E+07 302E+07 397E+07 397E+07 468E+07 216E+07 160E+07 129E+07 164E+07 300E+07 476E+07 271E+07 334E+07 347E+07 362E+07 345E+07 347E+07 DEOXYCARNITINE 133E+07 000E+00 157E+07 159E+07 291E+07 202E+07 151E+07 132E+07 206E+07 243E+07 123E+07 230E+07 195E+07 367E+07 153E+07 141E+07 170E+07 181E+07 162E+07 178E+07 214E+07 117E+07 221E+07 105E+07 173E+07 187E+07 187E+07 177E+07 176E+07 L-ASPARAGINE 644E+06 210E+05 263E+07 248E+07 838E+06 952E+06 595E+06 235E+07 247E+07 244E+07 597E+06 100E+07 761E+06 159E+07 154E+07 209E+07 210E+07 796E+06 830E+06 668E+06 665E+06 155E+07 230E+07 153E+07 147E+07 149E+07 159E+07 144E+07 144E+07 ETHANOLAMINE_PHOSPHATE 406E+06 000E+00 708E+06 415E+06 440E+06 474E+06 428E+06 773E+06 515E+06 433E+06 768E+06 108E+07 533E+06 526E+06 581E+06 505E+06 108E+07 394E+06 348E+06 399E+06 600E+06 444E+06 426E+06 103E+07 546E+06 622E+06 575E+06 574E+06 582E+06 GUANIDINOACETATE 145E+06 000E+00 162E+06 138E+06 197E+06 186E+06 156E+06 175E+06 195E+06 267E+06 205E+06 216E+06 148E+06 192E+06 661E+05 880E+05 148E+06 157E+06 156E+06 182E+06 167E+06 139E+06 260E+06 102E+06 159E+06 164E+06 181E+06 168E+06 158E+06 2-DEOXY-D-GLUCOSE 419E+08 129E+05 309E+08 380E+08 410E+08 410E+08 419E+08 350E+08 383E+08 319E+08 335E+08 422E+08 423E+08 380E+08 341E+08 389E+08 365E+08 324E+08 421E+08 397E+08 395E+08 322E+08 408E+08 341E+08 375E+08 386E+08 382E+08 369E+08 374E+08 TAURINE 419E+08 129E+05 309E+08 380E+08 410E+08 410E+08 419E+08 350E+08 383E+08 319E+08 335E+08 422E+08 423E+08 380E+08 341E+08 389E+08 365E+08 324E+08 421E+08 397E+08 395E+08 322E+08 408E+08 341E+08 375E+08 386E+08 382E+08 369E+08 374E+08 L-GLUTAMINE 598E+08 264E+05 365E+08 510E+08 706E+08 584E+08 503E+08 474E+08 495E+08 495E+08 419E+08 691E+08 643E+08 849E+08 300E+08 643E+08 455E+08 525E+08 595E+08 590E+08 617E+08 330E+08 586E+08 349E+08 512E+08 540E+08 549E+08 526E+08 524E+08 4-HYDROXY-PROLINE 203E+06 000E+00 130E+07 138E+07 327E+06 210E+06 149E+06 983E+06 900E+06 168E+07 147E+06 511E+06 162E+06 245E+06 443E+06 100E+06 119E+07 466E+06 333E+06 317E+06 273E+06 399E+06 960E+06 762E+05 488E+06 566E+06 489E+06 448E+06 540E+06 L-THREONINE 203E+07 903E+05 730E+07 613E+07 334E+07 220E+07 172E+07 696E+07 948E+07 737E+07 206E+07 375E+07 217E+07 238E+07 723E+07 744E+07 772E+07 345E+07 227E+07 220E+07 203E+07 355E+07 590E+07 442E+07 439E+07 458E+07 460E+07 442E+07 458E+07 PHOSPHOCHOLINE 474E+07 655E+04 850E+07 980E+07 753E+07 622E+07 461E+07 112E+08 135E+08 101E+08 924E+07 117E+08 743E+07 962E+07 889E+07 792E+07 108E+08 524E+07 632E+07 631E+07 627E+07 557E+07 940E+07 792E+07 835E+07 823E+07 798E+07 801E+07 794E+07 L-ASPARTATE 258E+07 126E+06 651E+07 585E+07 569E+07 774E+07 280E+07 870E+07 131E+08 880E+07 943E+07 684E+07 517E+07 466E+07 529E+07 105E+08 708E+07 278E+07 504E+07 427E+07 290E+07 616E+07 957E+07 753E+07 654E+07 668E+07 676E+07 652E+07 674E+07 L-GLUTAMIC_ACID 286E+08 868E+06 383E+08 503E+08 500E+08 359E+08 256E+08 414E+08 520E+08 512E+08 369E+08 468E+08 445E+08 363E+08 277E+08 354E+08 407E+08 312E+08 395E+08 386E+08 359E+08 314E+08 523E+08 195E+08 379E+08 375E+08 385E+08 370E+08 376E+08 1-NH2-CYCLOPROPANE-1-CARBOXYLATE 276E+07 722E+05 418E+07 494E+07 407E+07 317E+07 232E+07 433E+07 539E+07 506E+07 308E+07 421E+07 369E+07 322E+07 345E+07 421E+07 437E+07 291E+07 342E+07 337E+07 306E+07 300E+07 483E+07 247E+07 372E+07 375E+07 377E+07 362E+07 370E+07 GLYCERO-3-PHOSPHOCHOLINE 308E+07 633E+05 623E+07 657E+07 441E+07 373E+07 209E+07 690E+07 871E+07 765E+07 506E+07 688E+07 533E+07 428E+07 370E+07 472E+07 654E+07 292E+07 372E+07 365E+07 350E+07 529E+07 826E+07 360E+07 512E+07 488E+07 468E+07 446E+07 498E+07 CREATINE 544E+09 258E+05 284E+09 442E+09 746E+09 598E+09 487E+09 406E+09 597E+09 448E+09 342E+09 685E+09 632E+09 677E+09 336E+09 533E+09 395E+09 426E+09 538E+09 519E+09 551E+09 334E+09 614E+09 306E+09 513E+09 496E+09 483E+09 464E+09 484E+09 CREATININE 501E+07 000E+00 226E+07 355E+07 645E+07 511E+07 383E+07 337E+07 514E+07 385E+07 272E+07 582E+07 539E+07 574E+07 275E+07 452E+07 326E+07 379E+07 479E+07 437E+07 474E+07 281E+07 510E+07 243E+07 446E+07 421E+07 417E+07 380E+07 425E+07 CITRULLINE 123E+07 158E+05 161E+07 146E+07 193E+07 142E+07 108E+07 170E+07 196E+07 191E+07 105E+07 164E+07 149E+07 174E+07 190E+07 116E+07 149E+07 157E+07 115E+07 122E+07 144E+07 724E+06 155E+07 649E+06 140E+07 138E+07 142E+07 142E+07 141E+07 ALPHA-AMINOADIPATE 190E+06 361E+03 565E+06 506E+06 555E+06 184E+06 195E+06 496E+06 495E+06 830E+06 335E+06 470E+06 267E+06 275E+06 395E+06 202E+06 467E+06 657E+06 132E+06 216E+06 337E+06 498E+06 879E+06 278E+06 395E+06 406E+06 405E+06 372E+06 373E+06 TRIGONELLINE 575E+06 276E+05 163E+07 211E+07 112E+07 718E+06 373E+06 112E+07 289E+07 230E+07 356E+06 848E+06 454E+06 574E+06 782E+06 928E+06 147E+07 669E+06 480E+06 517E+06 677E+06 127E+07 240E+07 773E+06 113E+07 111E+07 105E+07 101E+07 104E+07 L-PROLINE 643E+07 724E+05 355E+08 294E+08 160E+08 662E+07 497E+07 237E+08 371E+08 317E+08 508E+07 133E+08 575E+07 958E+07 205E+08 233E+08 349E+08 103E+08 621E+07 887E+07 531E+07 126E+08 218E+08 139E+08 165E+08 157E+08 165E+08 162E+08 159E+08 ALLANTOIN 118E+06 290E+03 168E+06 137E+06 165E+06 105E+06 105E+06 119E+06 136E+06 118E+06 109E+06 136E+06 941E+05 137E+06 145E+06 146E+06 140E+06 175E+06 784E+05 100E+06 109E+06 143E+06 118E+06 163E+06 125E+06 128E+06 116E+06 128E+06 122E+06 4-GUANIDINOBUTANOATE 451E+05 205E+04 105E+06 568E+05 137E+06 835E+05 601E+05 720E+05 810E+05 115E+06 109E+06 988E+05 364E+05 751E+05 136E+06 174E+04 857E+05 152E+06 692E+05 889E+05 121E+06 338E+05 171E+05 243E+05 577E+05 837E+05 540E+05 595E+05 683E+05 CYTIDINE_5-DIPHOSPHOCHOLINE 152E+06 000E+00 884E+05 682E+05 255E+06 189E+06 107E+06 890E+05 109E+06 821E+05 898E+05 242E+06 177E+06 183E+06 343E+05 412E+05 827E+05 111E+06 127E+06 143E+06 118E+06 796E+05 159E+06 279E+05 117E+06 121E+06 108E+06 106E+06 114E+06 CYTOSINE 183E+07 000E+00 166E+07 304E+07 194E+07 207E+07 181E+07 271E+07 513E+07 258E+07 134E+07 249E+07 208E+07 200E+07 197E+07 515E+07 294E+07 132E+07 168E+07 160E+07 165E+07 265E+07 421E+07 203E+07 254E+07 237E+07 245E+07 238E+07 240E+07 ADENINE 810E+06 193E+05 810E+06 196E+07 202E+07 232E+07 102E+07 161E+07 425E+07 217E+07 105E+07 196E+07 192E+07 161E+07 952E+06 000E+00 356E+06 119E+07 141E+07 118E+07 112E+07 347E+06 256E+07 000E+00 128E+07 122E+07 118E+07 114E+07 117E+07 UROCANATE 274E+06 197E+06 205E+06 229E+06 607E+06 216E+06 253E+06 193E+06 505E+06 462E+06 364E+06 145E+06 148E+06 852E+05 119E+06 180E+06 616E+06 102E+06 123E+06 896E+05 126E+06 240E+06 186E+06 194E+06 623E+06 599E+06 606E+06 590E+06 584E+06 NICOTINAMIDE_MONONUCLEOTIDE 214E+06 000E+00 401E+06 622E+06 308E+06 554E+06 263E+06 649E+06 114E+07 776E+06 542E+06 514E+06 389E+06 324E+06 394E+06 131E+07 322E+06 378E+06 345E+06 201E+06 289E+06 168E+06 304E+06 126E+06 445E+06 398E+06 447E+06 495E+06 444E+06 CREATINE_PHOSPHATE 120E+07 000E+00 300E+07 260E+07 116E+07 542E+06 712E+06 340E+07 353E+07 281E+07 942E+06 207E+07 111E+07 136E+07 315E+07 495E+07 558E+07 839E+06 140E+07 139E+07 749E+06 414E+07 378E+07 287E+07 154E+07 157E+07 139E+07 124E+07 135E+07 DIHYDROOROTATE 324E+06 435E+03 178E+06 254E+06 292E+06 314E+06 246E+06 231E+06 290E+06 206E+06 198E+06 288E+06 307E+06 274E+06 189E+06 275E+06 202E+06 198E+06 229E+06 226E+06 243E+06 200E+06 284E+06 207E+06 233E+06 234E+06 230E+06 228E+06 240E+06 GLUCOSE_1-PHOSPHATE 400E+07 000E+00 277E+07 479E+07 222E+07 344E+07 329E+07 458E+07 481E+07 245E+07 346E+07 164E+07 317E+07 273E+07 390E+07 403E+07 336E+07 115E+07 313E+07 215E+07 183E+07 483E+07 660E+07 469E+07 278E+07 267E+07 273E+07 241E+07 246E+07 N-Acetyl-4-O-acetylneuraminic_acid 352E+06 157E+04 255E+06 454E+06 368E+06 398E+06 234E+06 332E+06 545E+06 367E+06 245E+06 451E+06 372E+06 325E+06 300E+06 494E+06 418E+06 240E+06 398E+06 392E+06 408E+06 340E+06 573E+06 489E+06 411E+06 400E+06 376E+06 391E+06 360E+06 GLYCEROL_3-PHOSPHATE 360E+07 155E+04 226E+07 271E+07 324E+07 301E+07 250E+07 252E+07 323E+07 321E+07 180E+07 425E+07 299E+07 377E+07 184E+07 272E+07 269E+07 177E+07 318E+07 361E+07 228E+07 206E+07 372E+07 102E+07 275E+07 285E+07 277E+07 266E+07 265E+07 L-PIPECOLIC_ACID 290E+06 524E+04 132E+07 106E+07 273E+06 224E+06 225E+06 517E+06 162E+07 166E+07 141E+06 424E+06 153E+06 297E+06 336E+06 121E+07 961E+06 382E+06 251E+06 319E+06 351E+06 747E+06 188E+07 783E+06 623E+06 630E+06 715E+06 710E+06 660E+06 L-METHIONINE 101E+07 000E+00 408E+07 426E+07 255E+07 939E+06 153E+06 426E+07 834E+07 453E+07 161E+06 321E+07 954E+06 125E+07 276E+07 417E+07 545E+07 199E+07 380E+05 495E+05 427E+06 131E+07 413E+07 178E+07 224E+07 225E+07 224E+07 232E+07 227E+07 LYXOSE 575E+05 187E+03 231E+06 240E+06 135E+06 512E+05 327E+04 246E+06 478E+06 268E+06 353E+04 192E+06 492E+05 649E+05 156E+06 237E+06 313E+06 116E+06 995E+03 431E+03 202E+05 750E+05 236E+06 110E+06 124E+06 127E+06 131E+06 135E+06 124E+06 GUANINE 469E+05 674E+03 199E+06 186E+06 117E+06 432E+05 336E+04 210E+06 356E+06 226E+06 189E+04 148E+06 422E+05 558E+05 134E+06 171E+06 237E+06 857E+05 118E+05 478E+03 168E+05 204E+06 196E+06 771E+05 901E+05 119E+06 110E+06 109E+06 106E+06 5,6-DIHYDRO_URACIL 366E+05 000E+00 124E+06 135E+06 294E+06 110E+06 685E+05 114E+06 176E+06 134E+06 106E+06 172E+06 524E+05 259E+06 834E+05 122E+06 119E+05 165E+06 230E+05 163E+06 988E+05 118E+06 145E+06 755E+05 132E+06 136E+06 133E+06 135E+06 135E+06 N-ACETYL-ASPARTIC_ACID 182E+06 322E+03 142E+06 113E+06 508E+06 326E+06 164E+06 251E+06 588E+06 231E+06 698E+06 870E+06 504E+06 470E+06 243E+06 296E+06 283E+06 368E+06 596E+06 737E+06 478E+06 112E+06 175E+06 191E+06 352E+06 329E+06 367E+06 356E+06 366E+06 HYPOXANTHINE 909E+07 173E+05 623E+07 247E+06 257E+08 134E+08 125E+08 817E+07 150E+04 546E+07 734E+07 113E+08 111E+08 161E+08 414E+07 103E+04 580E+03 220E+08 670E+07 107E+08 195E+08 108E+08 600E+06 660E+06 849E+07 825E+07 802E+07 807E+07 803E+07 L-TYROSINE 406E+07 329E+05 883E+07 766E+07 115E+08 385E+07 248E+07 647E+07 924E+07 799E+07 233E+07 918E+07 286E+07 714E+07 550E+07 571E+07 861E+07 737E+07 360E+07 358E+07 339E+07 406E+07 787E+07 429E+07 585E+07 570E+07 580E+07 573E+07 576E+07 NICOTINAMIDE 534E+08 000E+00 380E+08 613E+08 106E+09 657E+08 683E+08 458E+08 705E+08 411E+08 385E+08 738E+08 613E+08 709E+08 394E+08 703E+08 550E+08 750E+08 699E+08 691E+08 102E+09 461E+08 575E+08 499E+08 604E+08 595E+08 575E+08 562E+08 585E+08 ADENOSINE_5-MONOPHOSPHATE 557E+08 000E+00 190E+08 104E+08 523E+08 492E+08 328E+08 157E+08 139E+08 204E+08 331E+08 556E+08 444E+08 493E+08 134E+08 158E+08 208E+08 500E+08 348E+08 427E+08 373E+08 200E+08 250E+08 150E+08 345E+08 334E+08 337E+08 313E+08 318E+08 OPHTHALMIC_ACID 548E+05 000E+00 365E+06 227E+06 362E+06 881E+05 544E+05 302E+06 384E+06 347E+06 142E+06 121E+06 169E+06 815E+05 479E+06 198E+07 199E+06 299E+06 322E+05 720E+05 135E+06 271E+06 420E+06 975E+06 354E+06 329E+06 315E+06 305E+06 337E+06 XANTHINE 164E+07 343E+04 261E+07 153E+07 499E+07 305E+07 243E+07 404E+07 121E+07 302E+07 172E+07 353E+07 265E+07 299E+07 308E+07 000E+00 151E+07 390E+07 259E+07 274E+07 445E+07 340E+07 184E+07 362E+07 251E+07 247E+07 246E+07 273E+07 263E+07 URIDINE_5-MONOPHOSPHATE 806E+06 000E+00 772E+06 492E+06 685E+06 439E+06 451E+06 783E+06 745E+06 845E+06 507E+06 862E+06 582E+06 640E+06 769E+06 694E+06 101E+07 559E+06 491E+06 651E+06 127E+06 791E+06 958E+06 136E+07 658E+06 661E+06 656E+06 641E+06 672E+06 N-ACETYL-GLUTAMIC_ACID 215E+05 000E+00 359E+05 167E+05 319E+05 274E+05 187E+05 551E+05 317E+05 349E+05 739E+05 563E+05 114E+05 134E+05 150E+05 227E+05 398E+05 248E+05 183E+05 297E+05 152E+05 176E+05 212E+05 369E+05 310E+05 283E+05 241E+05 264E+05 288E+05 GUANOSINE_5-PHOSPHATE 229E+07 000E+00 164E+07 996E+06 266E+07 190E+07 191E+07 152E+07 131E+07 862E+06 120E+07 258E+07 197E+07 235E+07 165E+07 110E+07 164E+07 184E+07 116E+07 190E+07 282E+07 252E+07 219E+07 144E+07 187E+07 187E+07 188E+07 176E+07 186E+07 URIDINE 210E+06 000E+00 268E+06 562E+06 379E+06 311E+06 241E+06 518E+06 103E+07 550E+06 197E+06 405E+06 295E+06 299E+06 433E+06 120E+07 664E+06 236E+06 260E+06 222E+06 278E+06 301E+06 804E+06 377E+06 429E+06 432E+06 436E+06 425E+06 465E+06 URACIL 167E+07 000E+00 228E+07 436E+07 293E+07 239E+07 186E+07 404E+07 802E+07 417E+07 155E+07 326E+07 224E+07 244E+07 332E+07 914E+07 517E+07 200E+07 200E+07 172E+07 226E+07 249E+07 626E+07 302E+07 363E+07 349E+07 352E+07 331E+07 344E+07 ADENOSINE_3,5-DIPHOSPHATE 246E+07 000E+00 496E+04 700E+06 464E+07 170E+07 891E+06 919E+06 149E+07 216E+07 136E+07 592E+07 432E+07 377E+07 934E+06 113E+07 133E+07 196E+07 388E+07 318E+07 254E+07 921E+06 238E+07 758E+06 190E+07 205E+07 179E+07 179E+07 193E+07 INOSINE_5-MONOPHOSPHATE 164E+07 000E+00 547E+07 542E+07 125E+08 224E+07 978E+07 567E+07 424E+07 260E+07 207E+07 412E+07 593E+07 873E+07 667E+07 261E+07 528E+07 166E+07 231E+07 399E+07 846E+07 430E+07 287E+07 815E+07 531E+07 558E+07 606E+07 543E+07 530E+07 NAD 141E+07 000E+00 350E+06 430E+06 202E+07 157E+07 755E+06 603E+06 104E+07 106E+07 798E+06 249E+07 208E+07 210E+07 212E+06 553E+06 550E+06 703E+06 141E+07 147E+07 876E+06 290E+06 139E+07 109E+06 104E+07 979E+06 896E+06 865E+06 882E+06 GUANOSINE 288E+06 361E+03 267E+07 480E+07 140E+07 844E+06 538E+06 437E+07 811E+07 393E+07 651E+06 138E+07 650E+06 891E+06 378E+07 718E+07 407E+07 127E+07 719E+06 626E+06 115E+07 275E+07 650E+07 271E+07 262E+07 264E+07 264E+07 286E+07 281E+07 INOSINE 289E+07 000E+00 392E+07 839E+07 486E+07 307E+07 291E+07 667E+07 144E+08 489E+07 152E+07 427E+07 402E+07 327E+07 566E+07 128E+08 910E+07 329E+07 221E+07 293E+07 431E+07 397E+03 720E+07 620E+07 618E+07 654E+07 710E+07 783E+07 816E+07 L-PHENYLALANINE 698E+07 270E+05 156E+08 149E+08 163E+08 661E+07 401E+07 165E+08 297E+08 184E+08 467E+07 131E+08 716E+07 118E+08 246E+08 279E+08 246E+08 129E+08 568E+07 726E+07 720E+07 106E+08 159E+08 171E+08 129E+08 131E+08 130E+08 130E+08 133E+08 THYMINE 727E+05 000E+00 984E+05 135E+06 201E+06 100E+06 871E+05 158E+06 251E+06 106E+06 820E+05 241E+06 114E+06 157E+06 982E+05 217E+06 245E+06 116E+06 838E+05 778E+05 121E+06 138E+06 166E+06 170E+06 139E+06 135E+06 136E+06 134E+06 127E+06 L-TRYPTOPHAN 146E+07 403E+04 404E+07 299E+07 627E+07 190E+07 419E+06 267E+07 471E+07 399E+07 106E+07 283E+07 155E+07 260E+07 271E+07 287E+07 344E+07 536E+07 119E+07 108E+07 165E+07 245E+07 407E+07 213E+07 275E+07 279E+07 268E+07 266E+07 247E+07 PANTOTHENIC_ACID 227E+07 000E+00 829E+06 122E+07 717E+07 591E+07 294E+07 259E+07 362E+07 174E+07 315E+07 355E+07 609E+07 230E+07 345E+07 627E+07 198E+07 272E+07 249E+07 276E+07 266E+07 970E+06 182E+07 212E+07 291E+07 288E+07 290E+07 294E+07 290E+07 5-METHYLTHIOADENOSINE 235E+06 947E+03 497E+05 613E+05 918E+05 908E+05 863E+05 668E+05 141E+06 135E+06 909E+05 977E+05 660E+05 693E+05 378E+05 209E+04 289E+05 164E+06 793E+05 143E+06 682E+05 238E+06 968E+05 125E+04 456E+05 415E+05 428E+05 485E+05 502E+05 XANTHURENIC_ACID 168E+06 000E+00 343E+06 131E+06 426E+06 328E+06 339E+06 293E+06 147E+06 397E+06 332E+06 387E+06 387E+06 355E+06 294E+06 177E+06 157E+06 502E+06 231E+06 406E+06 356E+06 292E+06 137E+06 105E+06 237E+06 242E+06 277E+06 307E+06 315E+06 HIPPURATE 531E+05 000E+00 189E+06 904E+05 165E+06 796E+05 340E+05 164E+06 123E+06 150E+06 805E+05 661E+05 884E+05 225E+05 218E+06 242E+05 154E+06 169E+06 308E+05 766E+05 337E+05 191E+05 428E+05 182E+05 829E+05 784E+05 856E+05 786E+05 845E+05 RIBOFLAVIN 351E+05 000E+00 285E+05 396E+05 846E+05 340E+05 156E+05 328E+05 899E+05 466E+05 149E+05 575E+05 427E+05 304E+05 271E+05 693E+05 326E+05 310E+05 338E+05 550E+05 416E+05 394E+05 499E+05 283E+05 434E+05 452E+05 447E+05 438E+05 440E+05 FERULATE 217E+06 212E+06 285E+06 213E+06 267E+06 216E+06 219E+06 279E+06 223E+06 247E+06 252E+06 214E+06 212E+06 240E+06 275E+06 202E+06 229E+06 252E+06 199E+06 208E+06 240E+06 214E+06 215E+06 225E+06 196E+06 230E+06 208E+06 245E+06 219E+06 GLYCERALDEHYDE_3-P_DIETHYL_ACETAL 219E+08 566E+07 119E+08 263E+08 707E+07 311E+08 320E+08 926E+07 803E+07 433E+07 362E+08 717E+07 946E+07 630E+07 500E+07 231E+08 168E+08 594E+07 365E+08 302E+08 190E+08 143E+08 697E+07 663E+07 111E+08 109E+08 123E+08 132E+08 144E+08 LAUROYLCARNITINE 629E+06 000E+00 395E+05 717E+05 312E+06 159E+07 418E+06 105E+06 235E+06 923E+05 769E+06 872E+06 789E+06 584E+06 525E+05 375E+06 917E+05 372E+06 105E+07 897E+06 311E+06 116E+06 279E+06 728E+05 412E+06 424E+06 381E+06 363E+06 327E+06 OMEGA-HYDROXYDODECANOIC_ACID 903E+05 123E+06 981E+05 899E+05 915E+05 987E+05 900E+05 941E+05 958E+05 935E+05 936E+05 865E+05 905E+05 882E+05 932E+05 897E+05 882E+05 780E+05 860E+05 955E+05 943E+05 824E+05 970E+05 963E+05 893E+05 943E+05 975E+05 892E+05 898E+05 SPHINGANINE 227E+05 000E+00 356E+05 484E+05 107E+05 729E+04 781E+04 422E+05 555E+05 470E+05 152E+05 128E+05 103E+05 198E+05 277E+05 188E+05 451E+05 420E+05 200E+05 182E+05 229E+05 769E+05 989E+05 332E+05 297E+05 309E+05 285E+05 234E+05 216E+05 3-(4-HYDROXYPHENYL)PYRUVATE 276E+06 253E+06 255E+06 245E+06 250E+06 238E+06 234E+06 238E+06 224E+06 233E+06 237E+06 275E+06 247E+06 233E+06 245E+06 221E+06 235E+06 285E+06 249E+06 250E+06 255E+06 259E+06 271E+06 275E+06 276E+06 295E+06 251E+06 242E+06 234E+06 GLYCEROL_MYRISTATE 213E+06 189E+06 225E+06 220E+06 226E+06 236E+06 214E+06 219E+06 193E+06 209E+06 207E+06 208E+06 182E+06 191E+06 195E+06 201E+06 215E+06 194E+06 224E+06 224E+06 216E+06 228E+06 232E+06 247E+06 206E+06 207E+06 222E+06 188E+06 197E+06 LEUKOTRIENE_B4 227E+06 306E+06 252E+06 193E+06 187E+06 188E+06 177E+06 181E+06 180E+06 187E+06 192E+06 231E+06 181E+06 178E+06 187E+06 164E+06 152E+06 201E+06 167E+06 185E+06 192E+06 221E+06 200E+06 207E+06 246E+06 233E+06 192E+06 168E+06 159E+06 RETINOATE 115E+06 687E+03 978E+05 919E+05 265E+06 142E+06 941E+05 458E+05 474E+05 694E+05 109E+06 233E+06 809E+05 112E+06 300E+05 640E+05 158E+06 270E+06 266E+06 230E+06 232E+06 569E+05 133E+06 882E+05 145E+06 146E+06 108E+06 103E+06 885E+05 1-OLEOYL-RAC-GLYCEROL 122E+07 144E+05 467E+06 432E+06 671E+06 983E+06 854E+06 472E+06 474E+06 305E+06 976E+06 948E+06 330E+06 218E+06 312E+06 204E+07 114E+07 866E+06 144E+07 140E+07 111E+07 153E+07 115E+07 244E+07 110E+07 111E+07 854E+06 670E+06 616E+06 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name RT (min) SPERMIDINE 0.63221525 L-ORNITHINE 0.66787123 L-LYSINE 0.67232768 HISTAMINE 0.6959755 3-METHYLHISTAMINE 0.70934627 N,N,N-TRIMETHYL-LYSINE 0.73163195 DIETHANOLAMINE 0.73608795 CHOLINE 0.74500282 L-ARGININE 0.74945876 L-HISTIDINE 0.74945876 3-Methylhistidine 0.74945876 EPINEPHRINE 0.74945876 4-AMINOBUTANOATE 0.75391727 CARNOSINE 0.75391727 5-HYDROXYLYSINE 0.75391727 L-ANSERINE 0.75391727 L-CARNITINE 0.75837363 S-Adenosylmethionine 0.76728815 GLYCINE 0.77174446 L-SERINE 0.77620253 DEOXYCARNITINE 0.77620253 L-ASPARAGINE 0.77620253 ETHANOLAMINE_PHOSPHATE 0.78065895 GUANIDINOACETATE 0.78065895 2-DEOXY-D-GLUCOSE 0.78511532 TAURINE 0.78511532 L-GLUTAMINE 0.78957348 4-HYDROXY-PROLINE 0.78957348 L-THREONINE 0.79402992 PHOSPHOCHOLINE 0.79848827 L-ASPARTATE 0.79848827 L-GLUTAMIC_ACID 0.8029442 1-NH2-CYCLOPROPANE-1-CARBOXYLATE 0.8029442 GLYCERO-3-PHOSPHOCHOLINE 0.81185903 CREATINE 0.82522992 CREATININE 0.82522992 CITRULLINE 0.83860075 ALPHA-AMINOADIPATE 0.85642965 TRIGONELLINE 0.86980051 L-PROLINE 0.87425692 ALLANTOIN 0.87425692 4-GUANIDINOBUTANOATE 0.87425692 CYTIDINE_5-DIPHOSPHOCHOLINE 0.88317143 CYTOSINE 0.91882785 ADENINE 0.93348825 UROCANATE 0.93348825 NICOTINAMIDE_MONONUCLEOTIDE 0.93348825 CREATINE_PHOSPHATE 0.96468805 DIHYDROOROTATE 0.9736008 GLUCOSE_1-PHOSPHATE 0.9825154 N-Acetyl-4-O-acetylneuraminic_acid 0.99142968 GLYCEROL_3-PHOSPHATE 0.99588622 L-PIPECOLIC_ACID 1.0819446 L-METHIONINE 1.1502154 LYXOSE 1.1502154 GUANINE 1.1502154 5,6-DIHYDRO_URACIL 1.1962235 N-ACETYL-ASPARTIC_ACID 1.3535197 HYPOXANTHINE 1.4484614 L-TYROSINE 1.4484614 NICOTINAMIDE 1.5344779 ADENOSINE_5-MONOPHOSPHATE 1.5478487 OPHTHALMIC_ACID 1.6013341 XANTHINE 1.6637323 URIDINE_5-MONOPHOSPHATE 1.7052448 N-ACETYL-GLUTAMIC_ACID 1.7988428 GUANOSINE_5-PHOSPHATE 1.8746136 URIDINE 1.883528 URACIL 1.8879844 ADENOSINE_3,5-DIPHOSPHATE 2.1152929 INOSINE_5-MONOPHOSPHATE 2.2445468 NAD 2.4228305 GUANOSINE 3.0824724 INOSINE 3.1493287 L-PHENYLALANINE 3.390008 THYMINE 5.1906544 L-TRYPTOPHAN 5.2753378 PANTOTHENIC_ACID 5.6631005 5-METHYLTHIOADENOSINE 6.5663442 XANTHURENIC_ACID 6.7936527 HIPPURATE 7.1056468 RIBOFLAVIN 8.4930244 FERULATE 9.0869912 GLYCERALDEHYDE_3-P_DIETHYL_ACETAL 10.8604939999999 LAUROYLCARNITINE 10.998663 OMEGA-HYDROXYDODECANOIC_ACID 11.616109 SPHINGANINE 11.616109 3-(4-HYDROXYPHENYL)PYRUVATE 12.30184 GLYCEROL_MYRISTATE 13.2224679999999 LEUKOTRIENE_B4 13.657616 RETINOATE 13.770455 1-OLEOYL-RAC-GLYCEROL 14.1788469999999 METABOLITES_END #END