#METABOLOMICS WORKBENCH LarissaBRica_20230404_122656 DATATRACK_ID:3838 STUDY_ID:ST002554 ANALYSIS_ID:AN004205 PROJECT_ID:PR001646
VERSION             	1
CREATED_ON             	April 10, 2023, 9:50 am
#PROJECT
PR:PROJECT_TITLE                 	Untargeted lipidomic analysis of blood plasma samples from drug-naïve patients
PR:PROJECT_TITLE                 	with bipolar disorder and schizophrenia
PR:PROJECT_TYPE                  	Untargeted lipidomic analysis
PR:PROJECT_SUMMARY               	In this study, we obtained a lipidomic profile of plasma samples from patients
PR:PROJECT_SUMMARY               	with schizophrenia (SZ) and bipolar disorder (BD) in comparison to healthy
PR:PROJECT_SUMMARY               	controls (CT). The sample cohort consisted of 60 drug-naïve patients and 30
PR:PROJECT_SUMMARY               	control individuals. Untargeted lipidomics strategy using liquid chromatography
PR:PROJECT_SUMMARY               	coupled to high resolution mass spectrometry was employed to obtain the data,
PR:PROJECT_SUMMARY               	and univariate and multivariate statistical tools were applied to evaluate the
PR:PROJECT_SUMMARY               	results. Metabolic pathway networks were constructed and our results
PR:PROJECT_SUMMARY               	demonstrated alterations in different lipid pathways, such as
PR:PROJECT_SUMMARY               	glycerophospholipids, sphingolipids, and prostaglandins between schizophrenia
PR:PROJECT_SUMMARY               	and bipolar disorder patients.
PR:INSTITUTE                     	University of Campinas
PR:DEPARTMENT                    	Institute of Chemistry
PR:LABORATORY                    	LaBIOmics - Laboratory of Bioanalytics and Integrated Omics
PR:LAST_NAME                     	Brixner Riça
PR:FIRST_NAME                    	Larissa
PR:ADDRESS                       	Laboratório B-211 a 215 - Instituto de Química, R. Josué de Castro, 126-336 -
PR:ADDRESS                       	Cidade Universitária, Campinas - SP
PR:EMAIL                         	larissabrixner@gmail.com
PR:PHONE                         	+55 19 35213060
#STUDY
ST:STUDY_TITLE                   	Untargeted lipidomic analysis of blood plasma samples from drug-naïve patients
ST:STUDY_TITLE                   	with bipolar disorder and schizophrenia
ST:STUDY_TYPE                    	Untargeted lipidomic analysis
ST:STUDY_SUMMARY                 	In this study, we obtained a lipidomic profile of plasma samples from patients
ST:STUDY_SUMMARY                 	with schizophrenia (SZ) and bipolar disorder (BD) in comparison to healthy
ST:STUDY_SUMMARY                 	controls (CT). The sample cohort consisted of 60 drug-naïve patients and 30
ST:STUDY_SUMMARY                 	control individuals. Untargeted lipidomics strategy using liquid chromatography
ST:STUDY_SUMMARY                 	coupled to high resolution mass spectrometry was employed to obtain the data,
ST:STUDY_SUMMARY                 	and univariate and multivariate statistical tools were applied to evaluate the
ST:STUDY_SUMMARY                 	results. Metabolic pathway networks were constructed and our results
ST:STUDY_SUMMARY                 	demonstrated alterations in different lipid pathways, such as
ST:STUDY_SUMMARY                 	glycerophospholipids, sphingolipids, and prostaglandins between schizophrenia
ST:STUDY_SUMMARY                 	and bipolar disorder patients. The differential diagnosis is crucial for
ST:STUDY_SUMMARY                 	effective treatment and improving the quality of life of patients with psychotic
ST:STUDY_SUMMARY                 	disorders.
ST:INSTITUTE                     	University of Campinas
ST:DEPARTMENT                    	Institute of Chemistry
ST:LABORATORY                    	LaBIOmics - Laboratory of Bioanalytics and Integrated Omics
ST:LAST_NAME                     	Brixner Riça
ST:FIRST_NAME                    	Larissa
ST:ADDRESS                       	Laboratório B-211 a 215 - Instituto de Química, R. Josué de Castro, 126-336 -
ST:ADDRESS                       	Cidade Universitária, Campinas - SP
ST:EMAIL                         	larissabrixner@gmail.com
ST:PHONE                         	+55 19 35213060
ST:NUM_GROUPS                    	3
ST:TOTAL_SUBJECTS                	90
ST:NUM_MALES                     	42
ST:NUM_FEMALES                   	48
#SUBJECT
SU:SUBJECT_TYPE                  	Human
SU:SUBJECT_SPECIES               	Homo sapiens
SU:TAXONOMY_ID                   	9606
SU:AGE_OR_AGE_RANGE              	15-38
SU:GENDER                        	Male and female
SU:HUMAN_INCLUSION_CRITERIA      	--
SU:HUMAN_EXCLUSION_CRITERIA      	Subjects with other psychiatric or neurological disorders were excluded
#FACTORS
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data
SUBJECT_SAMPLE_FACTORS           	-	CT01	Group:Control	Gender=1; Age=31; Years_Education=11; RAW_FILE_NAME=CT01.mzML
SUBJECT_SAMPLE_FACTORS           	-	CT02	Group:Control	Gender=2; Age=23; Years_Education=15; RAW_FILE_NAME=CT02.mzML
SUBJECT_SAMPLE_FACTORS           	-	CT03	Group:Control	Gender=1; Age=28; Years_Education=13; RAW_FILE_NAME=CT03.mzML
SUBJECT_SAMPLE_FACTORS           	-	CT04	Group:Control	Gender=2; Age=28; Years_Education=11; RAW_FILE_NAME=CT04.mzML
SUBJECT_SAMPLE_FACTORS           	-	CT05	Group:Control	Gender=2; Age=30; Years_Education=15; RAW_FILE_NAME=CT05.mzML
SUBJECT_SAMPLE_FACTORS           	-	CT06	Group:Control	Gender=1; Age=31; Years_Education=11; RAW_FILE_NAME=CT06.mzML
SUBJECT_SAMPLE_FACTORS           	-	CT07	Group:Control	Gender=2; Age=23; Years_Education=15; RAW_FILE_NAME=CT07.mzML
SUBJECT_SAMPLE_FACTORS           	-	CT08	Group:Control	Gender=1; Age=28; Years_Education=21; RAW_FILE_NAME=CT08.mzML
SUBJECT_SAMPLE_FACTORS           	-	CT09	Group:Control	Gender=1; Age=24; Years_Education=8; RAW_FILE_NAME=CT09.mzML
SUBJECT_SAMPLE_FACTORS           	-	CT10	Group:Control	Gender=2; Age=23; Years_Education=13; RAW_FILE_NAME=CT10.mzML
SUBJECT_SAMPLE_FACTORS           	-	CT11	Group:Control	Gender=2; Age=27; Years_Education=17; RAW_FILE_NAME=CT11.mzML
SUBJECT_SAMPLE_FACTORS           	-	CT12	Group:Control	Gender=2; Age=26; Years_Education=14; RAW_FILE_NAME=CT12.mzML
SUBJECT_SAMPLE_FACTORS           	-	CT13	Group:Control	Gender=2; Age=26; Years_Education=14; RAW_FILE_NAME=CT13.mzML
SUBJECT_SAMPLE_FACTORS           	-	CT14	Group:Control	Gender=1; Age=26; Years_Education=12; RAW_FILE_NAME=CT14.mzML
SUBJECT_SAMPLE_FACTORS           	-	CT15	Group:Control	Gender=1; Age=23; Years_Education=16; RAW_FILE_NAME=CT15.mzML
SUBJECT_SAMPLE_FACTORS           	-	CT16	Group:Control	Gender=1; Age=26; Years_Education=12; RAW_FILE_NAME=CT16.mzML
SUBJECT_SAMPLE_FACTORS           	-	CT17	Group:Control	Gender=1; Age=29; Years_Education=15; RAW_FILE_NAME=CT17.mzML
SUBJECT_SAMPLE_FACTORS           	-	CT18	Group:Control	Gender=1; Age=23; Years_Education=16; RAW_FILE_NAME=CT18.mzML
SUBJECT_SAMPLE_FACTORS           	-	CT19	Group:Control	Gender=2; Age=27; Years_Education=17; RAW_FILE_NAME=CT19.mzML
SUBJECT_SAMPLE_FACTORS           	-	CT20	Group:Control	Gender=1; Age=26; Years_Education=15; RAW_FILE_NAME=CT20.mzML
SUBJECT_SAMPLE_FACTORS           	-	CT21	Group:Control	Gender=2; Age=27; Years_Education=15; RAW_FILE_NAME=CT21.mzML
SUBJECT_SAMPLE_FACTORS           	-	CT22	Group:Control	Gender=2; Age=27; Years_Education=18; RAW_FILE_NAME=CT22.mzML
SUBJECT_SAMPLE_FACTORS           	-	CT23	Group:Control	Gender=1; Age=27; Years_Education=12; RAW_FILE_NAME=CT23.mzML
SUBJECT_SAMPLE_FACTORS           	-	CT24	Group:Control	Gender=2; Age=27; Years_Education=15; RAW_FILE_NAME=CT24.mzML
SUBJECT_SAMPLE_FACTORS           	-	CT25	Group:Control	Gender=1; Age=27; Years_Education=17; RAW_FILE_NAME=CT25.mzML
SUBJECT_SAMPLE_FACTORS           	-	CT26	Group:Control	Gender=2; Age=27; Years_Education=11; RAW_FILE_NAME=CT26.mzML
SUBJECT_SAMPLE_FACTORS           	-	CT27	Group:Control	Gender=2; Age=27; Years_Education=18; RAW_FILE_NAME=CT27.mzML
SUBJECT_SAMPLE_FACTORS           	-	CT28	Group:Control	Gender=2; Age=25; Years_Education=11; RAW_FILE_NAME=CT28.mzML
SUBJECT_SAMPLE_FACTORS           	-	CT29	Group:Control	Gender=1; Age=27; Years_Education=15; RAW_FILE_NAME=CT29.mzML
SUBJECT_SAMPLE_FACTORS           	-	CT30	Group:Control	Gender=1; Age=27; Years_Education=17; RAW_FILE_NAME=CT30.mzML
SUBJECT_SAMPLE_FACTORS           	-	BD01	Group:BD	Gender=2; Age=34; Years_Education=-; RAW_FILE_NAME=BD01.mzML
SUBJECT_SAMPLE_FACTORS           	-	BD02	Group:BD	Gender=2; Age=32; Years_Education=16; RAW_FILE_NAME=BD02.mzML
SUBJECT_SAMPLE_FACTORS           	-	BD03	Group:BD	Gender=1; Age=23; Years_Education=11; RAW_FILE_NAME=BD03.mzML
SUBJECT_SAMPLE_FACTORS           	-	BD04	Group:BD	Gender=2; Age=26; Years_Education=-; RAW_FILE_NAME=BD04.mzML
SUBJECT_SAMPLE_FACTORS           	-	BD05	Group:BD	Gender=1; Age=23; Years_Education=11; RAW_FILE_NAME=BD05.mzML
SUBJECT_SAMPLE_FACTORS           	-	BD06	Group:BD	Gender=2; Age=17; Years_Education=10; RAW_FILE_NAME=BD06.mzML
SUBJECT_SAMPLE_FACTORS           	-	BD07	Group:BD	Gender=2; Age=28; Years_Education=11; RAW_FILE_NAME=BD07.mzML
SUBJECT_SAMPLE_FACTORS           	-	BD08	Group:BD	Gender=2; Age=23; Years_Education=15; RAW_FILE_NAME=BD08.mzML
SUBJECT_SAMPLE_FACTORS           	-	BD09	Group:BD	Gender=1; Age=30; Years_Education=15; RAW_FILE_NAME=BD09.mzML
SUBJECT_SAMPLE_FACTORS           	-	BD10	Group:BD	Gender=1; Age=29; Years_Education=11; RAW_FILE_NAME=BD10.mzML
SUBJECT_SAMPLE_FACTORS           	-	BD11	Group:BD	Gender=2; Age=23; Years_Education=15; RAW_FILE_NAME=BD11.mzML
SUBJECT_SAMPLE_FACTORS           	-	BD12	Group:BD	Gender=1; Age=30; Years_Education=15; RAW_FILE_NAME=BD12.mzML
SUBJECT_SAMPLE_FACTORS           	-	BD13	Group:BD	Gender=2; Age=27; Years_Education=11; RAW_FILE_NAME=BD13.mzML
SUBJECT_SAMPLE_FACTORS           	-	BD14	Group:BD	Gender=1; Age=29; Years_Education=11; RAW_FILE_NAME=BD14.mzML
SUBJECT_SAMPLE_FACTORS           	-	BD15	Group:BD	Gender=1; Age=30; Years_Education=11; RAW_FILE_NAME=BD15.mzML
SUBJECT_SAMPLE_FACTORS           	-	BD16	Group:BD	Gender=1; Age=21; Years_Education=10; RAW_FILE_NAME=BD16.mzML
SUBJECT_SAMPLE_FACTORS           	-	BD17	Group:BD	Gender=1; Age=30; Years_Education=11; RAW_FILE_NAME=BD17.mzML
SUBJECT_SAMPLE_FACTORS           	-	BD18	Group:BD	Gender=2; Age=22; Years_Education=11; RAW_FILE_NAME=BD18.mzML
SUBJECT_SAMPLE_FACTORS           	-	BD19	Group:BD	Gender=2; Age=33; Years_Education=11; RAW_FILE_NAME=BD19.mzML
SUBJECT_SAMPLE_FACTORS           	-	BD20	Group:BD	Gender=2; Age=25; Years_Education=14; RAW_FILE_NAME=BD20.mzML
SUBJECT_SAMPLE_FACTORS           	-	BD21	Group:BD	Gender=2; Age=27; Years_Education=13; RAW_FILE_NAME=BD21.mzML
SUBJECT_SAMPLE_FACTORS           	-	BD22	Group:BD	Gender=1; Age=21; Years_Education=10; RAW_FILE_NAME=BD22.mzML
SUBJECT_SAMPLE_FACTORS           	-	BD23	Group:BD	Gender=2; Age=22; Years_Education=11; RAW_FILE_NAME=BD23.mzML
SUBJECT_SAMPLE_FACTORS           	-	BD24	Group:BD	Gender=2; Age=19; Years_Education=12; RAW_FILE_NAME=BD24.mzML
SUBJECT_SAMPLE_FACTORS           	-	BD25	Group:BD	Gender=2; Age=26; Years_Education=13; RAW_FILE_NAME=BD25.mzML
SUBJECT_SAMPLE_FACTORS           	-	BD26	Group:BD	Gender=2; Age=31; Years_Education=12; RAW_FILE_NAME=BD26.mzML
SUBJECT_SAMPLE_FACTORS           	-	BD27	Group:BD	Gender=2; Age=31; Years_Education=17; RAW_FILE_NAME=BD27.mzML
SUBJECT_SAMPLE_FACTORS           	-	BD28	Group:BD	Gender=2; Age=27; Years_Education=15; RAW_FILE_NAME=BD28.mzML
SUBJECT_SAMPLE_FACTORS           	-	BD29	Group:BD	Gender=1; Age=31; Years_Education=15; RAW_FILE_NAME=BD29.mzML
SUBJECT_SAMPLE_FACTORS           	-	BD30	Group:BD	Gender=2; Age=27; Years_Education=15; RAW_FILE_NAME=BD30.mzML
SUBJECT_SAMPLE_FACTORS           	-	SZ01	Group:SZ	Gender=1; Age=30; Years_Education=10; RAW_FILE_NAME=SZ01.mzML
SUBJECT_SAMPLE_FACTORS           	-	SZ02	Group:SZ	Gender=1; Age=18; Years_Education=11; RAW_FILE_NAME=SZ02.mzML
SUBJECT_SAMPLE_FACTORS           	-	SZ03	Group:SZ	Gender=2; Age=21; Years_Education=11; RAW_FILE_NAME=SZ03.mzML
SUBJECT_SAMPLE_FACTORS           	-	SZ04	Group:SZ	Gender=2; Age=30; Years_Education=15; RAW_FILE_NAME=SZ04.mzML
SUBJECT_SAMPLE_FACTORS           	-	SZ05	Group:SZ	Gender=1; Age=28; Years_Education=15; RAW_FILE_NAME=SZ05.mzML
SUBJECT_SAMPLE_FACTORS           	-	SZ06	Group:SZ	Gender=1; Age=21; Years_Education=11; RAW_FILE_NAME=SZ06.mzML
SUBJECT_SAMPLE_FACTORS           	-	SZ07	Group:SZ	Gender=1; Age=21; Years_Education=11; RAW_FILE_NAME=SZ07.mzML
SUBJECT_SAMPLE_FACTORS           	-	SZ08	Group:SZ	Gender=1; Age=28; Years_Education=15; RAW_FILE_NAME=SZ08.mzML
SUBJECT_SAMPLE_FACTORS           	-	SZ09	Group:SZ	Gender=1; Age=21; Years_Education=11; RAW_FILE_NAME=SZ09.mzML
SUBJECT_SAMPLE_FACTORS           	-	SZ10	Group:SZ	Gender=1; Age=29; Years_Education=15; RAW_FILE_NAME=SZ10.mzML
SUBJECT_SAMPLE_FACTORS           	-	SZ11	Group:SZ	Gender=1; Age=29; Years_Education=15; RAW_FILE_NAME=SZ11.mzML
SUBJECT_SAMPLE_FACTORS           	-	SZ12	Group:SZ	Gender=2; Age=38; Years_Education=4; RAW_FILE_NAME=SZ12.mzML
SUBJECT_SAMPLE_FACTORS           	-	SZ13	Group:SZ	Gender=1; Age=37; Years_Education=8; RAW_FILE_NAME=SZ13.mzML
SUBJECT_SAMPLE_FACTORS           	-	SZ14	Group:SZ	Gender=1; Age=15; Years_Education=9; RAW_FILE_NAME=SZ14.mzML
SUBJECT_SAMPLE_FACTORS           	-	SZ15	Group:SZ	Gender=2; Age=27; Years_Education=13; RAW_FILE_NAME=SZ15.mzML
SUBJECT_SAMPLE_FACTORS           	-	SZ16	Group:SZ	Gender=1; Age=15; Years_Education=9; RAW_FILE_NAME=SZ16.mzML
SUBJECT_SAMPLE_FACTORS           	-	SZ17	Group:SZ	Gender=2; Age=27; Years_Education=13; RAW_FILE_NAME=SZ17.mzML
SUBJECT_SAMPLE_FACTORS           	-	SZ18	Group:SZ	Gender=1; Age=30; Years_Education=11; RAW_FILE_NAME=SZ18.mzML
SUBJECT_SAMPLE_FACTORS           	-	SZ19	Group:SZ	Gender=2; Age=25; Years_Education=15; RAW_FILE_NAME=SZ19.mzML
SUBJECT_SAMPLE_FACTORS           	-	SZ20	Group:SZ	Gender=2; Age=24; Years_Education=14; RAW_FILE_NAME=SZ20.mzML
SUBJECT_SAMPLE_FACTORS           	-	SZ21	Group:SZ	Gender=2; Age=38; Years_Education=4; RAW_FILE_NAME=SZ21.mzML
SUBJECT_SAMPLE_FACTORS           	-	SZ22	Group:SZ	Gender=2; Age=17; Years_Education=11; RAW_FILE_NAME=SZ22.mzML
SUBJECT_SAMPLE_FACTORS           	-	SZ23	Group:SZ	Gender=2; Age=24; Years_Education=14; RAW_FILE_NAME=SZ23.mzML
SUBJECT_SAMPLE_FACTORS           	-	SZ24	Group:SZ	Gender=1; Age=30; Years_Education=11; RAW_FILE_NAME=SZ24.mzML
SUBJECT_SAMPLE_FACTORS           	-	SZ25	Group:SZ	Gender=2; Age=25; Years_Education=15; RAW_FILE_NAME=SZ25.mzML
SUBJECT_SAMPLE_FACTORS           	-	SZ26	Group:SZ	Gender=2; Age=28; Years_Education=15; RAW_FILE_NAME=SZ26.mzML
SUBJECT_SAMPLE_FACTORS           	-	SZ27	Group:SZ	Gender=2; Age=28; Years_Education=15; RAW_FILE_NAME=SZ27.mzML
SUBJECT_SAMPLE_FACTORS           	-	SZ28	Group:SZ	Gender=1; Age=37; Years_Education=11; RAW_FILE_NAME=SZ28.mzML
SUBJECT_SAMPLE_FACTORS           	-	SZ29	Group:SZ	Gender=2; Age=17; Years_Education=11; RAW_FILE_NAME=SZ29.mzML
SUBJECT_SAMPLE_FACTORS           	-	SZ30	Group:SZ	Gender=1; Age=37; Years_Education=11; RAW_FILE_NAME=SZ30.mzML
SUBJECT_SAMPLE_FACTORS           	-	QC_1_2	Group:QC	Gender=na; Age=na; Years_Education=na; RAW_FILE_NAME=QC_1_2.mzML
SUBJECT_SAMPLE_FACTORS           	-	QC_2	Group:QC	Gender=na; Age=na; Years_Education=na; RAW_FILE_NAME=QC_2.mzML
SUBJECT_SAMPLE_FACTORS           	-	QC_2_2	Group:QC	Gender=na; Age=na; Years_Education=na; RAW_FILE_NAME=QC_2_2.mzML
SUBJECT_SAMPLE_FACTORS           	-	QC_2_3	Group:QC	Gender=na; Age=na; Years_Education=na; RAW_FILE_NAME=QC_2_3.mzML
SUBJECT_SAMPLE_FACTORS           	-	QC_2_4	Group:QC	Gender=na; Age=na; Years_Education=na; RAW_FILE_NAME=QC_2_4.mzML
SUBJECT_SAMPLE_FACTORS           	-	QC_3	Group:QC	Gender=na; Age=na; Years_Education=na; RAW_FILE_NAME=QC_3.mzML
SUBJECT_SAMPLE_FACTORS           	-	QC_4	Group:QC	Gender=na; Age=na; Years_Education=na; RAW_FILE_NAME=QC_4.mzML
SUBJECT_SAMPLE_FACTORS           	-	QC_4_2	Group:QC	Gender=na; Age=na; Years_Education=na; RAW_FILE_NAME=QC_4_2.mzML
SUBJECT_SAMPLE_FACTORS           	-	QC_4_3	Group:QC	Gender=na; Age=na; Years_Education=na; RAW_FILE_NAME=QC_4_3.mzML
SUBJECT_SAMPLE_FACTORS           	-	QC_5	Group:QC	Gender=na; Age=na; Years_Education=na; RAW_FILE_NAME=QC_5.mzML
SUBJECT_SAMPLE_FACTORS           	-	QC_5_2	Group:QC	Gender=na; Age=na; Years_Education=na; RAW_FILE_NAME=QC_5_2.mzML
SUBJECT_SAMPLE_FACTORS           	-	QC_5_3	Group:QC	Gender=na; Age=na; Years_Education=na; RAW_FILE_NAME=QC_5_3.mzML
SUBJECT_SAMPLE_FACTORS           	-	QC_5_4	Group:QC	Gender=na; Age=na; Years_Education=na; RAW_FILE_NAME=QC_5_4.mzML
#COLLECTION
CO:COLLECTION_SUMMARY            	Blood samples of all subjects were collected in EDTA-coated tubes (BD
CO:COLLECTION_SUMMARY            	Vacuntainer®, Becton Dickinson, Franklin Lakes, USA) for plasma metabolite
CO:COLLECTION_SUMMARY            	determination after 8 hours of fasting. Samples were centrifuged at 20 °C at
CO:COLLECTION_SUMMARY            	1800 g for 15 minutes and were were stored at -80 ºC prior to lipid extraction.
CO:SAMPLE_TYPE                   	Blood (plasma)
CO:STORAGE_CONDITIONS            	-80℃
#TREATMENT
TR:TREATMENT_SUMMARY             	The study was conducted at the Institute of Psychiatry, University of Sao Paulo,
TR:TREATMENT_SUMMARY             	Brazil. The sample consisted of 60 drug-naïve patients (30 SZ and 30 BD) and 30
TR:TREATMENT_SUMMARY             	healthy controls (CT). All participants were under 60 years old and were
TR:TREATMENT_SUMMARY             	middle-income, community-dwelling subjects from the hospital catchment area. SZ
TR:TREATMENT_SUMMARY             	diagnosis was established according to the Diagnostic and Statistical Manual of
TR:TREATMENT_SUMMARY             	Mental Disorders (DSM-IV) (Bell, 1994) and SCID-I/P-Structured Clinical
TR:TREATMENT_SUMMARY             	Interview Disorders Axis I for DSM-IV version 2.0 (First, 1997) was used to
TR:TREATMENT_SUMMARY             	confirm the diagnosis. Psychopathology was assessed using the Positive and
TR:TREATMENT_SUMMARY             	Negative Symptoms Scale (PANSS) (Kay et al., 1987) including the positive and
TR:TREATMENT_SUMMARY             	negative subscales and general psychopathology. Depressive and manic symptoms
TR:TREATMENT_SUMMARY             	were assessed for BD patients by the Hamilton Depression Rating Scale (HAM-D)
TR:TREATMENT_SUMMARY             	(Hamilton, 1960) and Young's Mania Rating Scale (YMRS) (Young et al., 1978).
TR:TREATMENT_SUMMARY             	Subjects with other psychiatric or neurological disorders were excluded.
TR:TREATMENT_SUMMARY             	References cited: Bell, C.C. DSM-IV: Diagnostic and Statistical Manual of Mental
TR:TREATMENT_SUMMARY             	Disorders. JAMA 1994, 272, 828–829. Spitzer, M.; Robert, L.; Gibbon, M.;
TR:TREATMENT_SUMMARY             	Williams, J. Structured Clinical Interview for DSM-IV-TR Axis I Disorders,
TR:TREATMENT_SUMMARY             	Research Version, Non-Patient Edition (SCID-I/NP). New York: Biometrics
TR:TREATMENT_SUMMARY             	Research, New York State Psychiatric Institute 2002. Kay, S.R.; Fiszbein, A.;
TR:TREATMENT_SUMMARY             	Opler, L.A. The Positive and Negative Syndrome Scale (PANSS) for Schizophrenia.
TR:TREATMENT_SUMMARY             	Schizophr Bull 1987, 13, 261–276. Hamilton, M. A RATING SCALE FOR DEPRESSION.
TR:TREATMENT_SUMMARY             	J Neurol Neurosurg Psychiatry 1960, 23, 56–62.
TR:HUMAN_FASTING                 	8 h
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	Plasma lipids were extracted using the SIMPLEX method (Coman et al., 2016, DOI:
SP:SAMPLEPREP_SUMMARY            	10.1074/mcp.m115.053702). Briefly, the plasma samples were incubated with cold
SP:SAMPLEPREP_SUMMARY            	methanol and MTBE, followed by the addition of a 0.1% (m/v) ammonium acetate
SP:SAMPLEPREP_SUMMARY            	solution to induce phase separation. The supernatant con-taining lipids, was
SP:SAMPLEPREP_SUMMARY            	collected and the solvents were removed with a vacuum concentrator (Eppendorf,
SP:SAMPLEPREP_SUMMARY            	Hamburg, Germany). The microtubes with the extracted lipids were stored at -80
SP:SAMPLEPREP_SUMMARY            	ºC prior to UHPLC-MS analysis.
SP:EXTRACTION_METHOD             	Simultaneous Metabolite, Protein, Lipid Extraction (SIMPLEX)
SP:EXTRACT_STORAGE               	-80℃
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_SUMMARY        	Ultra-high performance liquid chromatography coupled to mass spectrometry
CH:CHROMATOGRAPHY_SUMMARY        	(UHPLC-MS) analyses were performed on an UltiMate 3000 UHPLC system (Thermo
CH:CHROMATOGRAPHY_SUMMARY        	Fisher Scientific, Waltham, MA, USA) coupled to a QExactive Orbitrap mass
CH:CHROMATOGRAPHY_SUMMARY        	spectrometer (Thermo Fisher Scientific). The chromatographic separation was done
CH:CHROMATOGRAPHY_SUMMARY        	on an ACQUITY CSH C18 (2.1 ⨯ 100 mm, 1.7 μm) column (Waters, Milford, MA,
CH:CHROMATOGRAPHY_SUMMARY        	USA) and the temperature of the column oven was set to 55 ºC. The mobile phase
CH:CHROMATOGRAPHY_SUMMARY        	A consisted of an ACN:water mixture (60:40) with 1 mmol/L ammonium formate and
CH:CHROMATOGRAPHY_SUMMARY        	0.1% (v/v) formic acid, and the mobile phase B consisted of an IPA:ACN mixture
CH:CHROMATOGRAPHY_SUMMARY        	(90:10) with 1 mmol/L ammonium formate and 0.1% (v/v) formic acid. The flow rate
CH:CHROMATOGRAPHY_SUMMARY        	was 0.4 mL/min and the injection volume was 5 μL for each sample. The gradient
CH:CHROMATOGRAPHY_SUMMARY        	elution program consisted of a first linear gradient from solvent (A/B: 60/40)
CH:CHROMATOGRAPHY_SUMMARY        	to solvent (A/B: 57/43) over 2 min; a rapid increase to solvent (A/B: 50/50); a
CH:CHROMATOGRAPHY_SUMMARY        	second linear gradient to solvent (A/B: 46/54) over 10 min; a rapid increase to
CH:CHROMATOGRAPHY_SUMMARY        	solvent (A/B: 30/70); a third linear gradient to solvent (A/B: 1/99) over 6 min;
CH:CHROMATOGRAPHY_SUMMARY        	a rapid de-crease to solvent (A/B: 60/40); and finally, an isocratic elution of
CH:CHROMATOGRAPHY_SUMMARY        	the solvent (A/B: 60/40) for 2 min. The column was equilibrated with solvent
CH:CHROMATOGRAPHY_SUMMARY        	(A/B: 60/40) for 5 min before reuse. The total run time was 25 min for each
CH:CHROMATOGRAPHY_SUMMARY        	analysis. Samples were randomized prior to analysis. To verify system stability,
CH:CHROMATOGRAPHY_SUMMARY        	quality control (QC) samples were injected at the start and at the end of a run
CH:CHROMATOGRAPHY_SUMMARY        	and after every 10th sample.
CH:CHROMATOGRAPHY_TYPE           	Reversed phase
CH:INSTRUMENT_NAME               	UltiMate 3000 UHPLC system (Thermo Fisher Scientific)
CH:COLUMN_NAME                   	ACQUITY CSH C18 (2.1 ⨯ 100 mm, 1.7 μm) column (Waters)
CH:SOLVENT_A                     	60% acetonitrile/40% water; 1 mM ammonium formate; 0.1% formic acid
CH:SOLVENT_B                     	90% isopropanol/10% acetonitrile; 1mM ammonium formate; 0.1% formic acid
CH:FLOW_GRADIENT                 	Gradient elution program (view Summary)
CH:FLOW_RATE                     	0.4 mL/min
CH:COLUMN_TEMPERATURE            	55
CH:SAMPLE_INJECTION              	5 μL
#ANALYSIS
AN:ANALYSIS_TYPE                 	MS
#MS
MS:INSTRUMENT_NAME               	Thermo Q Exactive Orbitrap
MS:INSTRUMENT_TYPE               	Orbitrap
MS:MS_TYPE                       	ESI
MS:ION_MODE                      	NEGATIVE
MS:MS_COMMENTS                   	MS scans were acquired in electrospray ionization (ESI) negative mode from m/z
MS:MS_COMMENTS                   	100 to 1500 with a resolution of 70,000. MS target values and maximum injection
MS:MS_COMMENTS                   	time were 3 ⨯ 106 ions and 200 ms, respectively. The .raw files were converted
MS:MS_COMMENTS                   	to .mzML with the MSConvert software (ProteoWizard, Palo Alto, CA, USA). Using
MS:MS_COMMENTS                   	RStudio (RStudio, Boston, MA, USA), the parameters for data processing with the
MS:MS_COMMENTS                   	‘xcms’ package were optimized with the ‘IPO’ package based on QC
MS:MS_COMMENTS                   	samples. With the optimized parameters, all the files were processed with
MS:MS_COMMENTS                   	‘xcms’ and, afterwards, the data were organized in a peak list with the
MS:MS_COMMENTS                   	‘CAMERA’ package, followed by the median fold change normalization.
MS:MS_RESULTS_FILE               	ST002554_AN004205_Results.txt	UNITS:Intensity	Has m/z:Yes	Has RT:Yes	RT units:Seconds
#END