#METABOLOMICS WORKBENCH wpathmasiri_20230413_064055 DATATRACK_ID:3859 STUDY_ID:ST002559 ANALYSIS_ID:AN004218
VERSION                          	1
CREATED_ON                       	04-14-2023
#PROJECT
PR:PROJECT_TITLE                 	The ECHO Cohort Exposome: First Steps using HHEAR Analysis – An Opportunity
PR:PROJECT_TITLE                 	for ALL ECHO Cohorts to Contribute Type A Samples – Untargeted Analysis (VCSIP
PR:PROJECT_TITLE                 	Cohort)
PR:PROJECT_TYPE                  	C18 Reversed-Phase Broad Spectrum Metabolomics
PR:PROJECT_SUMMARY               	This project was funded by the NIH Environmental influences on Child Health
PR:PROJECT_SUMMARY               	Outcomes (ECHO) Program. Untargeted metabolomics profiling data was acquired
PR:PROJECT_SUMMARY               	from urine samples provided by 14 ECHO cohorts.
PR:INSTITUTE                     	NC HHEAR Hub
PR:DEPARTMENT                    	Untargeted Analysis
PR:LABORATORY                    	Sumner Lab
PR:LAST_NAME                     	Rushing
PR:FIRST_NAME                    	Blake
PR:ADDRESS                       	Nutrition Research Institute, UNC-CH, 500 Laureate Way, Kannapolis, NC 28081
PR:EMAIL                         	blake_rushing@unc.edu
PR:PHONE                         	(704) 282-9838
PR:FUNDING_SOURCE                	This research was supported by the Environmental influences on Child Health
PR:FUNDING_SOURCE                	Outcomes (ECHO) program, Office of The Director, National Institutes of Health.
PR:FUNDING_SOURCE                	The content is solely the responsibility of the authors and does not necessarily
PR:FUNDING_SOURCE                	represent the official views of the National Institutes of Health. The VCSIP
PR:FUNDING_SOURCE                	ECHO Cohort is supported by the following ECHO Program Collaborators: ECHO
PR:FUNDING_SOURCE                	Coordinating Center: Duke Clinical Research Institute, Durham, North Carolina:
PR:FUNDING_SOURCE                	Smith PB, Newby KL, Benjamin DK; U2C OD023375; ECHO Data Analysis Center: Johns
PR:FUNDING_SOURCE                	Hopkins University Bloomberg School of Public Health, Baltimore, Maryland:
PR:FUNDING_SOURCE                	Jacobson LP; Research Triangle Institute, Durham, North Carolina: Catellier.D 
PR:FUNDING_SOURCE                	U24 OD023382; North Carolina Human Health Exposure Analysis Resource Hub:
PR:FUNDING_SOURCE                	Research Triangle Institute: Fennell T, University of North Carolina at Chapel
PR:FUNDING_SOURCE                	Hill: Sumner S, University of North Carolina at Charlotte: Du X; U2C ES030857;
PR:FUNDING_SOURCE                	Human Health Exposure Analysis Resource Coordinating Center: Westat, Inc.,
PR:FUNDING_SOURCE                	Rockville, Maryland: O’Brien B; U24 ES026539
PR:DOI                           	http://dx.doi.org/10.21228/M8971G
#STUDY
ST:STUDY_TITLE                   	The ECHO Cohort Exposome: First Steps using HHEAR Analysis – An Opportunity
ST:STUDY_TITLE                   	for ALL ECHO Cohorts to Contribute Type A Samples – Untargeted Analysis (VCSIP
ST:STUDY_TITLE                   	Cohort)
ST:STUDY_TYPE                    	Prospective Cohort Study
ST:STUDY_SUMMARY                 	Maternal smoking during pregnancy negatively effects fetal lung development and
ST:STUDY_SUMMARY                 	causes lifelong decreases in offspring pulmonary function and offspring
ST:STUDY_SUMMARY                 	respiratory health including increased risk of childhood asthma development. 
ST:STUDY_SUMMARY                 	The VCSIP cohort was created from 2 RCTs to determine if vitamin C
ST:STUDY_SUMMARY                 	supplementation to pregnant smokers could ameliorate some of the negative
ST:STUDY_SUMMARY                 	effects of maternal smoking during pregnancy on offspring lung health (JAMA
ST:STUDY_SUMMARY                 	311:2074-2082, 2014; JAMA Pediatr 177:16-24, 2023).  Both pre- and postnatal
ST:STUDY_SUMMARY                 	tobacco exposures have been carefully monitored and extensive respiratory
ST:STUDY_SUMMARY                 	phenotyping on the offspring have been performed.  Please contact Cindy McEvoy
ST:STUDY_SUMMARY                 	at Mcevoyc@ohsu.edu for questions related to subject characteristics and
ST:STUDY_SUMMARY                 	outcomes.
 This research was supported by the Environmental influences on Child
ST:STUDY_SUMMARY                 	Health Outcomes (ECHO) Program, Office of The Director, National Institutes of
ST:STUDY_SUMMARY                 	Health. The content is solely the responsibility of the authors and does not
ST:STUDY_SUMMARY                 	necessarily represent the official views of the National Institutes of Health. 
ST:STUDY_SUMMARY                 	VCSIP is an ECHO cohort which is supported by the following ECHO Program
ST:STUDY_SUMMARY                 	Collaborators:
 ECHO Coordinating Center: Duke Clinical Research Institute,
ST:STUDY_SUMMARY                 	Durham, North Carolina: Smith PB, Newby KL, Benjamin DK; U2C OD023375
 ECHO Data
ST:STUDY_SUMMARY                 	Analysis Center: Johns Hopkins University Bloomberg School of Public Health,
ST:STUDY_SUMMARY                 	Baltimore, Maryland: Jacobson LP; Research Triangle Institute, Durham, North
ST:STUDY_SUMMARY                 	Carolina: Catellier.D  U24 OD023382
 North Carolina Human Health Exposure
ST:STUDY_SUMMARY                 	Analysis Resource Hub:  Research Triangle Institute: Fennell T, University of
ST:STUDY_SUMMARY                 	North Carolina at Chapel Hill: Sumner S, University of North Carolina at
ST:STUDY_SUMMARY                 	Charlotte:  Du X; U2C ES030857
 Human Health Exposure Analysis Resource
ST:STUDY_SUMMARY                 	Coordinating Center: Westat, Inc., Rockville, Maryland: O’Brien B; U24
ST:STUDY_SUMMARY                 	ES026539
ST:INSTITUTE                     	Oregon Health & Science University
ST:DEPARTMENT                    	Division of Neuroscience
ST:LAST_NAME                     	Spindel
ST:FIRST_NAME                    	Eliot
ST:ADDRESS                       	3181 SW Sam Jackson Park Road Portland, OR 97239-3098
ST:EMAIL                         	spindele@ohsu.edu
ST:PHONE                         	503-346-5524
ST:SUBMIT_DATE                   	2023-04-13
#SUBJECT
SU:SUBJECT_TYPE                  	Human
SU:SUBJECT_SPECIES               	Homo sapiens
SU:TAXONOMY_ID                   	9606
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data
SUBJECT_SAMPLE_FACTORS           	-	VCSIP_B_8	Sample Type:Blank | Batch:1 | Analysis Order:108	RAW_FILE_NAME=VCSIP_B_8
SUBJECT_SAMPLE_FACTORS           	-	VCSIP_B_1	Sample Type:Blank | Batch:1 | Analysis Order:12	RAW_FILE_NAME=VCSIP_B_1
SUBJECT_SAMPLE_FACTORS           	-	VCSIP_B_2	Sample Type:Blank | Batch:1 | Analysis Order:26	RAW_FILE_NAME=VCSIP_B_2
SUBJECT_SAMPLE_FACTORS           	-	VCSIP_B_3	Sample Type:Blank | Batch:1 | Analysis Order:39	RAW_FILE_NAME=VCSIP_B_3
SUBJECT_SAMPLE_FACTORS           	-	VCSIP_B_4	Sample Type:Blank | Batch:1 | Analysis Order:53	RAW_FILE_NAME=VCSIP_B_4
SUBJECT_SAMPLE_FACTORS           	-	VCSIP_B_5	Sample Type:Blank | Batch:1 | Analysis Order:67	RAW_FILE_NAME=VCSIP_B_5
SUBJECT_SAMPLE_FACTORS           	-	VCSIP_B_6	Sample Type:Blank | Batch:1 | Analysis Order:81	RAW_FILE_NAME=VCSIP_B_6
SUBJECT_SAMPLE_FACTORS           	-	VCSIP_B_7	Sample Type:Blank | Batch:1 | Analysis Order:94	RAW_FILE_NAME=VCSIP_B_7
SUBJECT_SAMPLE_FACTORS           	-	VCSIP_B_9	Sample Type:Blank | Batch:2 | Analysis Order:123	RAW_FILE_NAME=VCSIP_B_9
SUBJECT_SAMPLE_FACTORS           	-	VCSIP_B_10	Sample Type:Blank | Batch:2 | Analysis Order:137	RAW_FILE_NAME=VCSIP_B_10
SUBJECT_SAMPLE_FACTORS           	-	VCSIP_B_11	Sample Type:Blank | Batch:2 | Analysis Order:150	RAW_FILE_NAME=VCSIP_B_11
SUBJECT_SAMPLE_FACTORS           	-	VCSIP_B_12	Sample Type:Blank | Batch:2 | Analysis Order:164	RAW_FILE_NAME=VCSIP_B_12
SUBJECT_SAMPLE_FACTORS           	-	VCSIP_B_13	Sample Type:Blank | Batch:2 | Analysis Order:178	RAW_FILE_NAME=VCSIP_B_13
SUBJECT_SAMPLE_FACTORS           	-	VCSIP_B_14	Sample Type:Blank | Batch:2 | Analysis Order:192	RAW_FILE_NAME=VCSIP_B_14
SUBJECT_SAMPLE_FACTORS           	-	VCSIP_B_15	Sample Type:Blank | Batch:2 | Analysis Order:205	RAW_FILE_NAME=VCSIP_B_15
SUBJECT_SAMPLE_FACTORS           	-	VCSIP_B_16	Sample Type:Blank | Batch:2 | Analysis Order:219	RAW_FILE_NAME=VCSIP_B_16
SUBJECT_SAMPLE_FACTORS           	-	VCSIP_B_17	Sample Type:Blank | Batch:3 | Analysis Order:234	RAW_FILE_NAME=VCSIP_B_17
SUBJECT_SAMPLE_FACTORS           	-	VCSIP_B_18	Sample Type:Blank | Batch:3 | Analysis Order:248	RAW_FILE_NAME=VCSIP_B_18
SUBJECT_SAMPLE_FACTORS           	-	VCSIP_B_19	Sample Type:Blank | Batch:3 | Analysis Order:262	RAW_FILE_NAME=VCSIP_B_19
SUBJECT_SAMPLE_FACTORS           	-	VCSIP_B_20	Sample Type:Blank | Batch:3 | Analysis Order:276	RAW_FILE_NAME=VCSIP_B_20
SUBJECT_SAMPLE_FACTORS           	-	VCSIP_CHEAR_U_1	Sample Type:CHEAR Urine Pool | Batch:1 | Analysis Order:14	RAW_FILE_NAME=VCSIP_CHEAR_U_1
SUBJECT_SAMPLE_FACTORS           	-	VCSIP_CHEAR_U_2	Sample Type:CHEAR Urine Pool | Batch:1 | Analysis Order:41	RAW_FILE_NAME=VCSIP_CHEAR_U_2
SUBJECT_SAMPLE_FACTORS           	-	VCSIP_CHEAR_U_3	Sample Type:CHEAR Urine Pool | Batch:1 | Analysis Order:69	RAW_FILE_NAME=VCSIP_CHEAR_U_3
SUBJECT_SAMPLE_FACTORS           	-	VCSIP_CHEAR_U_4	Sample Type:CHEAR Urine Pool | Batch:1 | Analysis Order:96	RAW_FILE_NAME=VCSIP_CHEAR_U_4
SUBJECT_SAMPLE_FACTORS           	-	VCSIP_CHEAR_U_5	Sample Type:CHEAR Urine Pool | Batch:2 | Analysis Order:125	RAW_FILE_NAME=VCSIP_CHEAR_U_5
SUBJECT_SAMPLE_FACTORS           	-	VCSIP_CHEAR_U_6	Sample Type:CHEAR Urine Pool | Batch:2 | Analysis Order:152	RAW_FILE_NAME=VCSIP_CHEAR_U_6
SUBJECT_SAMPLE_FACTORS           	-	VCSIP_CHEAR_U_7	Sample Type:CHEAR Urine Pool | Batch:2 | Analysis Order:180	RAW_FILE_NAME=VCSIP_CHEAR_U_7
SUBJECT_SAMPLE_FACTORS           	-	VCSIP_CHEAR_U_8	Sample Type:CHEAR Urine Pool | Batch:2 | Analysis Order:207	RAW_FILE_NAME=VCSIP_CHEAR_U_8
SUBJECT_SAMPLE_FACTORS           	-	VCSIP_CHEAR_U_9	Sample Type:CHEAR Urine Pool | Batch:3 | Analysis Order:236	RAW_FILE_NAME=VCSIP_CHEAR_U_9
SUBJECT_SAMPLE_FACTORS           	-	VCSIP_CHEAR_U_10	Sample Type:CHEAR Urine Pool | Batch:3 | Analysis Order:264	RAW_FILE_NAME=VCSIP_CHEAR_U_10
SUBJECT_SAMPLE_FACTORS           	-	VCSIP_HHEAR_U1_1	Sample Type:HHEAR Urine Pool 1 (HHEAR_U1_20) | Batch:1 | Analysis Order:13	RAW_FILE_NAME=VCSIP_HHEAR_U1_1
SUBJECT_SAMPLE_FACTORS           	-	VCSIP_HHEAR_U1_2	Sample Type:HHEAR Urine Pool 1 (HHEAR_U1_20) | Batch:1 | Analysis Order:40	RAW_FILE_NAME=VCSIP_HHEAR_U1_2
SUBJECT_SAMPLE_FACTORS           	-	VCSIP_HHEAR_U1_3	Sample Type:HHEAR Urine Pool 1 (HHEAR_U1_20) | Batch:1 | Analysis Order:68	RAW_FILE_NAME=VCSIP_HHEAR_U1_3
SUBJECT_SAMPLE_FACTORS           	-	VCSIP_HHEAR_U1_4	Sample Type:HHEAR Urine Pool 1 (HHEAR_U1_20) | Batch:1 | Analysis Order:95	RAW_FILE_NAME=VCSIP_HHEAR_U1_4
SUBJECT_SAMPLE_FACTORS           	-	VCSIP_HHEAR_U1_5	Sample Type:HHEAR Urine Pool 1 (HHEAR_U1_20) | Batch:2 | Analysis Order:124	RAW_FILE_NAME=VCSIP_HHEAR_U1_5
SUBJECT_SAMPLE_FACTORS           	-	VCSIP_HHEAR_U1_6	Sample Type:HHEAR Urine Pool 1 (HHEAR_U1_20) | Batch:2 | Analysis Order:151	RAW_FILE_NAME=VCSIP_HHEAR_U1_6
SUBJECT_SAMPLE_FACTORS           	-	VCSIP_HHEAR_U1_7	Sample Type:HHEAR Urine Pool 1 (HHEAR_U1_20) | Batch:2 | Analysis Order:179	RAW_FILE_NAME=VCSIP_HHEAR_U1_7
SUBJECT_SAMPLE_FACTORS           	-	VCSIP_HHEAR_U1_8	Sample Type:HHEAR Urine Pool 1 (HHEAR_U1_20) | Batch:2 | Analysis Order:206	RAW_FILE_NAME=VCSIP_HHEAR_U1_8
SUBJECT_SAMPLE_FACTORS           	-	VCSIP_HHEAR_U1_9	Sample Type:HHEAR Urine Pool 1 (HHEAR_U1_20) | Batch:3 | Analysis Order:235	RAW_FILE_NAME=VCSIP_HHEAR_U1_9
SUBJECT_SAMPLE_FACTORS           	-	VCSIP_HHEAR_U1_10	Sample Type:HHEAR Urine Pool 1 (HHEAR_U1_20) | Batch:3 | Analysis Order:263	RAW_FILE_NAME=VCSIP_HHEAR_U1_10
SUBJECT_SAMPLE_FACTORS           	-	VCSIP_HHEAR_U2_4	Sample Type:HHEAR Urine Pool 2 (HHEAR_U2_50) | Batch:1 | Analysis Order:109	RAW_FILE_NAME=VCSIP_HHEAR_U2_4
SUBJECT_SAMPLE_FACTORS           	-	VCSIP_HHEAR_U2_1	Sample Type:HHEAR Urine Pool 2 (HHEAR_U2_50) | Batch:1 | Analysis Order:27	RAW_FILE_NAME=VCSIP_HHEAR_U2_1
SUBJECT_SAMPLE_FACTORS           	-	VCSIP_HHEAR_U2_2	Sample Type:HHEAR Urine Pool 2 (HHEAR_U2_50) | Batch:1 | Analysis Order:54	RAW_FILE_NAME=VCSIP_HHEAR_U2_2
SUBJECT_SAMPLE_FACTORS           	-	VCSIP_HHEAR_U2_3	Sample Type:HHEAR Urine Pool 2 (HHEAR_U2_50) | Batch:1 | Analysis Order:82	RAW_FILE_NAME=VCSIP_HHEAR_U2_3
SUBJECT_SAMPLE_FACTORS           	-	VCSIP_HHEAR_U2_5	Sample Type:HHEAR Urine Pool 2 (HHEAR_U2_50) | Batch:2 | Analysis Order:138	RAW_FILE_NAME=VCSIP_HHEAR_U2_5
SUBJECT_SAMPLE_FACTORS           	-	VCSIP_HHEAR_U2_6	Sample Type:HHEAR Urine Pool 2 (HHEAR_U2_50) | Batch:2 | Analysis Order:165	RAW_FILE_NAME=VCSIP_HHEAR_U2_6
SUBJECT_SAMPLE_FACTORS           	-	VCSIP_HHEAR_U2_7	Sample Type:HHEAR Urine Pool 2 (HHEAR_U2_50) | Batch:2 | Analysis Order:193	RAW_FILE_NAME=VCSIP_HHEAR_U2_7
SUBJECT_SAMPLE_FACTORS           	-	VCSIP_HHEAR_U2_8	Sample Type:HHEAR Urine Pool 2 (HHEAR_U2_50) | Batch:2 | Analysis Order:220	RAW_FILE_NAME=VCSIP_HHEAR_U2_8
SUBJECT_SAMPLE_FACTORS           	-	VCSIP_HHEAR_U2_9	Sample Type:HHEAR Urine Pool 2 (HHEAR_U2_50) | Batch:3 | Analysis Order:249	RAW_FILE_NAME=VCSIP_HHEAR_U2_9
SUBJECT_SAMPLE_FACTORS           	-	VCSIP_HHEAR_U2_10	Sample Type:HHEAR Urine Pool 2 (HHEAR_U2_50) | Batch:3 | Analysis Order:277	RAW_FILE_NAME=VCSIP_HHEAR_U2_10
SUBJECT_SAMPLE_FACTORS           	-	VCSIP_NIST_3672_1	Sample Type:NIST Urine Pool 3672 | Batch:1 | Analysis Order:1	RAW_FILE_NAME=VCSIP_NIST_3672_1
SUBJECT_SAMPLE_FACTORS           	-	VCSIP_NIST_3672_3	Sample Type:NIST Urine Pool 3672 | Batch:1 | Analysis Order:111	RAW_FILE_NAME=VCSIP_NIST_3672_3
SUBJECT_SAMPLE_FACTORS           	-	VCSIP_NIST_3672_2	Sample Type:NIST Urine Pool 3672 | Batch:1 | Analysis Order:56	RAW_FILE_NAME=VCSIP_NIST_3672_2
SUBJECT_SAMPLE_FACTORS           	-	VCSIP_NIST_3672_4	Sample Type:NIST Urine Pool 3672 | Batch:2 | Analysis Order:112	RAW_FILE_NAME=VCSIP_NIST_3672_4
SUBJECT_SAMPLE_FACTORS           	-	VCSIP_NIST_3672_5	Sample Type:NIST Urine Pool 3672 | Batch:2 | Analysis Order:167	RAW_FILE_NAME=VCSIP_NIST_3672_5
SUBJECT_SAMPLE_FACTORS           	-	VCSIP_NIST_3672_6	Sample Type:NIST Urine Pool 3672 | Batch:2 | Analysis Order:222	RAW_FILE_NAME=VCSIP_NIST_3672_6
SUBJECT_SAMPLE_FACTORS           	-	VCSIP_NIST_3672_7	Sample Type:NIST Urine Pool 3672 | Batch:3 | Analysis Order:223	RAW_FILE_NAME=VCSIP_NIST_3672_7
SUBJECT_SAMPLE_FACTORS           	-	VCSIP_NIST_3672_8	Sample Type:NIST Urine Pool 3672 | Batch:3 | Analysis Order:251	RAW_FILE_NAME=VCSIP_NIST_3672_8
SUBJECT_SAMPLE_FACTORS           	-	VCSIP_NIST_3672_9	Sample Type:NIST Urine Pool 3672 | Batch:3 | Analysis Order:279	RAW_FILE_NAME=VCSIP_NIST_3672_9
SUBJECT_SAMPLE_FACTORS           	-	VCSIP_SP_8	Sample Type:Study Pool | Batch:1 | Analysis Order:110	RAW_FILE_NAME=VCSIP_SP_8
SUBJECT_SAMPLE_FACTORS           	-	VCSIP_SP_1	Sample Type:Study Pool | Batch:1 | Analysis Order:15	RAW_FILE_NAME=VCSIP_SP_1
SUBJECT_SAMPLE_FACTORS           	-	VCSIP_SP_2	Sample Type:Study Pool | Batch:1 | Analysis Order:28	RAW_FILE_NAME=VCSIP_SP_2
SUBJECT_SAMPLE_FACTORS           	-	VCSIP_SP_3	Sample Type:Study Pool | Batch:1 | Analysis Order:42	RAW_FILE_NAME=VCSIP_SP_3
SUBJECT_SAMPLE_FACTORS           	-	VCSIP_SP_4	Sample Type:Study Pool | Batch:1 | Analysis Order:55	RAW_FILE_NAME=VCSIP_SP_4
SUBJECT_SAMPLE_FACTORS           	-	VCSIP_SP_5	Sample Type:Study Pool | Batch:1 | Analysis Order:70	RAW_FILE_NAME=VCSIP_SP_5
SUBJECT_SAMPLE_FACTORS           	-	VCSIP_SP_6	Sample Type:Study Pool | Batch:1 | Analysis Order:83	RAW_FILE_NAME=VCSIP_SP_6
SUBJECT_SAMPLE_FACTORS           	-	VCSIP_SP_7	Sample Type:Study Pool | Batch:1 | Analysis Order:97	RAW_FILE_NAME=VCSIP_SP_7
SUBJECT_SAMPLE_FACTORS           	-	VCSIP_SP_9	Sample Type:Study Pool | Batch:2 | Analysis Order:126	RAW_FILE_NAME=VCSIP_SP_9
SUBJECT_SAMPLE_FACTORS           	-	VCSIP_SP_10	Sample Type:Study Pool | Batch:2 | Analysis Order:139	RAW_FILE_NAME=VCSIP_SP_10
SUBJECT_SAMPLE_FACTORS           	-	VCSIP_SP_11	Sample Type:Study Pool | Batch:2 | Analysis Order:153	RAW_FILE_NAME=VCSIP_SP_11
SUBJECT_SAMPLE_FACTORS           	-	VCSIP_SP_12	Sample Type:Study Pool | Batch:2 | Analysis Order:166	RAW_FILE_NAME=VCSIP_SP_12
SUBJECT_SAMPLE_FACTORS           	-	VCSIP_SP_13	Sample Type:Study Pool | Batch:2 | Analysis Order:181	RAW_FILE_NAME=VCSIP_SP_13
SUBJECT_SAMPLE_FACTORS           	-	VCSIP_SP_14	Sample Type:Study Pool | Batch:2 | Analysis Order:194	RAW_FILE_NAME=VCSIP_SP_14
SUBJECT_SAMPLE_FACTORS           	-	VCSIP_SP_15	Sample Type:Study Pool | Batch:2 | Analysis Order:208	RAW_FILE_NAME=VCSIP_SP_15
SUBJECT_SAMPLE_FACTORS           	-	VCSIP_SP_16	Sample Type:Study Pool | Batch:2 | Analysis Order:221	RAW_FILE_NAME=VCSIP_SP_16
SUBJECT_SAMPLE_FACTORS           	-	VCSIP_SP_17	Sample Type:Study Pool | Batch:3 | Analysis Order:237	RAW_FILE_NAME=VCSIP_SP_17
SUBJECT_SAMPLE_FACTORS           	-	VCSIP_SP_18	Sample Type:Study Pool | Batch:3 | Analysis Order:250	RAW_FILE_NAME=VCSIP_SP_18
SUBJECT_SAMPLE_FACTORS           	-	VCSIP_SP_19	Sample Type:Study Pool | Batch:3 | Analysis Order:265	RAW_FILE_NAME=VCSIP_SP_19
SUBJECT_SAMPLE_FACTORS           	-	VCSIP_SP_20	Sample Type:Study Pool | Batch:3 | Analysis Order:278	RAW_FILE_NAME=VCSIP_SP_20
SUBJECT_SAMPLE_FACTORS           	EM-6GK72-U-00-12401	VCSIP_S_9	Sample Type:Study Sample | Batch:1 | Analysis Order:10	RAW_FILE_NAME=VCSIP_S_9
SUBJECT_SAMPLE_FACTORS           	EM-6GN87-U-00-12401	VCSIP_S_73	Sample Type:Study Sample | Batch:1 | Analysis Order:100	RAW_FILE_NAME=VCSIP_S_73
SUBJECT_SAMPLE_FACTORS           	EM-5DPA2-U-00-12401	VCSIP_S_74	Sample Type:Study Sample | Batch:1 | Analysis Order:101	RAW_FILE_NAME=VCSIP_S_74
SUBJECT_SAMPLE_FACTORS           	EM-6G9A8-U-00-12401	VCSIP_S_75	Sample Type:Study Sample | Batch:1 | Analysis Order:102	RAW_FILE_NAME=VCSIP_S_75
SUBJECT_SAMPLE_FACTORS           	EM-6GDV1-U-00-12401	VCSIP_S_76	Sample Type:Study Sample | Batch:1 | Analysis Order:103	RAW_FILE_NAME=VCSIP_S_76
SUBJECT_SAMPLE_FACTORS           	EM-6GD61-U-00-12401	VCSIP_S_77	Sample Type:Study Sample | Batch:1 | Analysis Order:104	RAW_FILE_NAME=VCSIP_S_77
SUBJECT_SAMPLE_FACTORS           	EM-6GAK8-U-00-12401	VCSIP_S_78	Sample Type:Study Sample | Batch:1 | Analysis Order:105	RAW_FILE_NAME=VCSIP_S_78
SUBJECT_SAMPLE_FACTORS           	EM-5DFD5-U-00-12401	VCSIP_S_79	Sample Type:Study Sample | Batch:1 | Analysis Order:106	RAW_FILE_NAME=VCSIP_S_79
SUBJECT_SAMPLE_FACTORS           	EM-5DVN7-U-00-12401	VCSIP_S_80	Sample Type:Study Sample | Batch:1 | Analysis Order:107	RAW_FILE_NAME=VCSIP_S_80
SUBJECT_SAMPLE_FACTORS           	EM-5DN01-U-00-12401	VCSIP_S_10	Sample Type:Study Sample | Batch:1 | Analysis Order:11	RAW_FILE_NAME=VCSIP_S_10
SUBJECT_SAMPLE_FACTORS           	EM-5E1B9-U-00-12401	VCSIP_S_11	Sample Type:Study Sample | Batch:1 | Analysis Order:16	RAW_FILE_NAME=VCSIP_S_11
SUBJECT_SAMPLE_FACTORS           	EM-6GCM2-U-00-12401	VCSIP_S_12	Sample Type:Study Sample | Batch:1 | Analysis Order:17	RAW_FILE_NAME=VCSIP_S_12
SUBJECT_SAMPLE_FACTORS           	EM-5DLY3-U-00-12401	VCSIP_S_13	Sample Type:Study Sample | Batch:1 | Analysis Order:18	RAW_FILE_NAME=VCSIP_S_13
SUBJECT_SAMPLE_FACTORS           	EM-5DQ32-U-00-12401	VCSIP_S_14	Sample Type:Study Sample | Batch:1 | Analysis Order:19	RAW_FILE_NAME=VCSIP_S_14
SUBJECT_SAMPLE_FACTORS           	EM-5DN68-U-00-12401	VCSIP_S_1	Sample Type:Study Sample | Batch:1 | Analysis Order:2	RAW_FILE_NAME=VCSIP_S_1
SUBJECT_SAMPLE_FACTORS           	EM-6GGC0-U-00-12401	VCSIP_S_15	Sample Type:Study Sample | Batch:1 | Analysis Order:20	RAW_FILE_NAME=VCSIP_S_15
SUBJECT_SAMPLE_FACTORS           	EM-6G8T7-U-00-12401	VCSIP_S_16	Sample Type:Study Sample | Batch:1 | Analysis Order:21	RAW_FILE_NAME=VCSIP_S_16
SUBJECT_SAMPLE_FACTORS           	EM-5DZB0-U-00-12401	VCSIP_S_17	Sample Type:Study Sample | Batch:1 | Analysis Order:22	RAW_FILE_NAME=VCSIP_S_17
SUBJECT_SAMPLE_FACTORS           	EM-6GG27-U-00-12401	VCSIP_S_18	Sample Type:Study Sample | Batch:1 | Analysis Order:23	RAW_FILE_NAME=VCSIP_S_18
SUBJECT_SAMPLE_FACTORS           	EM-5E5L3-U-00-12401	VCSIP_S_19	Sample Type:Study Sample | Batch:1 | Analysis Order:24	RAW_FILE_NAME=VCSIP_S_19
SUBJECT_SAMPLE_FACTORS           	EM-5E314-U-00-12401	VCSIP_S_20	Sample Type:Study Sample | Batch:1 | Analysis Order:25	RAW_FILE_NAME=VCSIP_S_20
SUBJECT_SAMPLE_FACTORS           	EM-6GNU3-U-00-12401	VCSIP_S_21	Sample Type:Study Sample | Batch:1 | Analysis Order:29	RAW_FILE_NAME=VCSIP_S_21
SUBJECT_SAMPLE_FACTORS           	EM-5E4E9-U-00-12401	VCSIP_S_2	Sample Type:Study Sample | Batch:1 | Analysis Order:3	RAW_FILE_NAME=VCSIP_S_2
SUBJECT_SAMPLE_FACTORS           	EM-6GUW2-U-00-12401	VCSIP_S_22	Sample Type:Study Sample | Batch:1 | Analysis Order:30	RAW_FILE_NAME=VCSIP_S_22
SUBJECT_SAMPLE_FACTORS           	EM-5DJ13-U-00-12401	VCSIP_S_23	Sample Type:Study Sample | Batch:1 | Analysis Order:31	RAW_FILE_NAME=VCSIP_S_23
SUBJECT_SAMPLE_FACTORS           	EM-5DQ73-U-00-12401	VCSIP_S_24	Sample Type:Study Sample | Batch:1 | Analysis Order:32	RAW_FILE_NAME=VCSIP_S_24
SUBJECT_SAMPLE_FACTORS           	EM-5DH07-U-00-12401	VCSIP_S_25	Sample Type:Study Sample | Batch:1 | Analysis Order:33	RAW_FILE_NAME=VCSIP_S_25
SUBJECT_SAMPLE_FACTORS           	EM-5DEH8-U-00-12401	VCSIP_S_26	Sample Type:Study Sample | Batch:1 | Analysis Order:34	RAW_FILE_NAME=VCSIP_S_26
SUBJECT_SAMPLE_FACTORS           	EM-5DG65-U-00-12401	VCSIP_S_27	Sample Type:Study Sample | Batch:1 | Analysis Order:35	RAW_FILE_NAME=VCSIP_S_27
SUBJECT_SAMPLE_FACTORS           	EM-6GF85-U-00-12401	VCSIP_S_28	Sample Type:Study Sample | Batch:1 | Analysis Order:36	RAW_FILE_NAME=VCSIP_S_28
SUBJECT_SAMPLE_FACTORS           	EM-5E4K6-U-00-12401	VCSIP_S_29	Sample Type:Study Sample | Batch:1 | Analysis Order:37	RAW_FILE_NAME=VCSIP_S_29
SUBJECT_SAMPLE_FACTORS           	EM-6GN12-U-00-12401	VCSIP_S_30	Sample Type:Study Sample | Batch:1 | Analysis Order:38	RAW_FILE_NAME=VCSIP_S_30
SUBJECT_SAMPLE_FACTORS           	EM-6GET4-U-00-12401	VCSIP_S_3	Sample Type:Study Sample | Batch:1 | Analysis Order:4	RAW_FILE_NAME=VCSIP_S_3
SUBJECT_SAMPLE_FACTORS           	EM-6GEG3-U-00-12401	VCSIP_S_31	Sample Type:Study Sample | Batch:1 | Analysis Order:43	RAW_FILE_NAME=VCSIP_S_31
SUBJECT_SAMPLE_FACTORS           	EM-6GSU8-U-00-12401	VCSIP_S_32	Sample Type:Study Sample | Batch:1 | Analysis Order:44	RAW_FILE_NAME=VCSIP_S_32
SUBJECT_SAMPLE_FACTORS           	EM-6G905-U-00-12401	VCSIP_S_33	Sample Type:Study Sample | Batch:1 | Analysis Order:45	RAW_FILE_NAME=VCSIP_S_33
SUBJECT_SAMPLE_FACTORS           	EM-5E2V4-U-00-12401	VCSIP_S_34	Sample Type:Study Sample | Batch:1 | Analysis Order:46	RAW_FILE_NAME=VCSIP_S_34
SUBJECT_SAMPLE_FACTORS           	EM-6GXG4-U-00-12401	VCSIP_S_35	Sample Type:Study Sample | Batch:1 | Analysis Order:47	RAW_FILE_NAME=VCSIP_S_35
SUBJECT_SAMPLE_FACTORS           	EM-5DFS2-U-00-12401	VCSIP_S_36	Sample Type:Study Sample | Batch:1 | Analysis Order:48	RAW_FILE_NAME=VCSIP_S_36
SUBJECT_SAMPLE_FACTORS           	EM-6GDW9-U-00-12401	VCSIP_S_37	Sample Type:Study Sample | Batch:1 | Analysis Order:49	RAW_FILE_NAME=VCSIP_S_37
SUBJECT_SAMPLE_FACTORS           	EM-5DV11-U-00-12401	VCSIP_S_4	Sample Type:Study Sample | Batch:1 | Analysis Order:5	RAW_FILE_NAME=VCSIP_S_4
SUBJECT_SAMPLE_FACTORS           	EM-5E2U6-U-00-12401	VCSIP_S_38	Sample Type:Study Sample | Batch:1 | Analysis Order:50	RAW_FILE_NAME=VCSIP_S_38
SUBJECT_SAMPLE_FACTORS           	EM-6G8N0-U-00-12401	VCSIP_S_39	Sample Type:Study Sample | Batch:1 | Analysis Order:51	RAW_FILE_NAME=VCSIP_S_39
SUBJECT_SAMPLE_FACTORS           	EM-5E2Q5-U-00-12401	VCSIP_S_40	Sample Type:Study Sample | Batch:1 | Analysis Order:52	RAW_FILE_NAME=VCSIP_S_40
SUBJECT_SAMPLE_FACTORS           	EM-6GNE8-U-00-12401	VCSIP_S_41	Sample Type:Study Sample | Batch:1 | Analysis Order:57	RAW_FILE_NAME=VCSIP_S_41
SUBJECT_SAMPLE_FACTORS           	EM-5DVE7-U-00-12401	VCSIP_S_42	Sample Type:Study Sample | Batch:1 | Analysis Order:58	RAW_FILE_NAME=VCSIP_S_42
SUBJECT_SAMPLE_FACTORS           	EM-5DND7-U-00-12401	VCSIP_S_43	Sample Type:Study Sample | Batch:1 | Analysis Order:59	RAW_FILE_NAME=VCSIP_S_43
SUBJECT_SAMPLE_FACTORS           	EM-6GE03-U-00-12401	VCSIP_S_5	Sample Type:Study Sample | Batch:1 | Analysis Order:6	RAW_FILE_NAME=VCSIP_S_5
SUBJECT_SAMPLE_FACTORS           	EM-6GX95-U-00-12401	VCSIP_S_44	Sample Type:Study Sample | Batch:1 | Analysis Order:60	RAW_FILE_NAME=VCSIP_S_44
SUBJECT_SAMPLE_FACTORS           	EM-5DGP8-U-00-12401	VCSIP_S_45	Sample Type:Study Sample | Batch:1 | Analysis Order:61	RAW_FILE_NAME=VCSIP_S_45
SUBJECT_SAMPLE_FACTORS           	EM-5DPF1-U-00-12401	VCSIP_S_46	Sample Type:Study Sample | Batch:1 | Analysis Order:62	RAW_FILE_NAME=VCSIP_S_46
SUBJECT_SAMPLE_FACTORS           	EM-5E3P6-U-00-12401	VCSIP_S_47	Sample Type:Study Sample | Batch:1 | Analysis Order:63	RAW_FILE_NAME=VCSIP_S_47
SUBJECT_SAMPLE_FACTORS           	EM-5DEN4-U-00-12401	VCSIP_S_48	Sample Type:Study Sample | Batch:1 | Analysis Order:64	RAW_FILE_NAME=VCSIP_S_48
SUBJECT_SAMPLE_FACTORS           	EM-5E2J0-U-00-12401	VCSIP_S_49	Sample Type:Study Sample | Batch:1 | Analysis Order:65	RAW_FILE_NAME=VCSIP_S_49
SUBJECT_SAMPLE_FACTORS           	EM-5DGU7-U-00-12401	VCSIP_S_50	Sample Type:Study Sample | Batch:1 | Analysis Order:66	RAW_FILE_NAME=VCSIP_S_50
SUBJECT_SAMPLE_FACTORS           	EM-6GME9-U-00-12401	VCSIP_S_6	Sample Type:Study Sample | Batch:1 | Analysis Order:7	RAW_FILE_NAME=VCSIP_S_6
SUBJECT_SAMPLE_FACTORS           	EM-5E751-U-00-12401	VCSIP_S_51	Sample Type:Study Sample | Batch:1 | Analysis Order:71	RAW_FILE_NAME=VCSIP_S_51
SUBJECT_SAMPLE_FACTORS           	EM-6GKQ5-U-00-12401	VCSIP_S_52	Sample Type:Study Sample | Batch:1 | Analysis Order:72	RAW_FILE_NAME=VCSIP_S_52
SUBJECT_SAMPLE_FACTORS           	EM-6GPJ5-U-00-12401	VCSIP_S_53	Sample Type:Study Sample | Batch:1 | Analysis Order:73	RAW_FILE_NAME=VCSIP_S_53
SUBJECT_SAMPLE_FACTORS           	EM-5DLX5-U-00-12401	VCSIP_S_54	Sample Type:Study Sample | Batch:1 | Analysis Order:74	RAW_FILE_NAME=VCSIP_S_54
SUBJECT_SAMPLE_FACTORS           	EM-6GQU0-U-00-12401	VCSIP_S_55	Sample Type:Study Sample | Batch:1 | Analysis Order:75	RAW_FILE_NAME=VCSIP_S_55
SUBJECT_SAMPLE_FACTORS           	EM-5DFJ2-U-00-12401	VCSIP_S_56	Sample Type:Study Sample | Batch:1 | Analysis Order:76	RAW_FILE_NAME=VCSIP_S_56
SUBJECT_SAMPLE_FACTORS           	EM-5E512-U-00-12401	VCSIP_S_57	Sample Type:Study Sample | Batch:1 | Analysis Order:77	RAW_FILE_NAME=VCSIP_S_57
SUBJECT_SAMPLE_FACTORS           	EM-5DRV4-U-00-12401	VCSIP_S_58	Sample Type:Study Sample | Batch:1 | Analysis Order:78	RAW_FILE_NAME=VCSIP_S_58
SUBJECT_SAMPLE_FACTORS           	EM-6GVJ9-U-00-12401	VCSIP_S_59	Sample Type:Study Sample | Batch:1 | Analysis Order:79	RAW_FILE_NAME=VCSIP_S_59
SUBJECT_SAMPLE_FACTORS           	EM-6GUQ5-U-00-12401	VCSIP_S_7	Sample Type:Study Sample | Batch:1 | Analysis Order:8	RAW_FILE_NAME=VCSIP_S_7
SUBJECT_SAMPLE_FACTORS           	EM-5E0H7-U-00-12401	VCSIP_S_60	Sample Type:Study Sample | Batch:1 | Analysis Order:80	RAW_FILE_NAME=VCSIP_S_60
SUBJECT_SAMPLE_FACTORS           	EM-6GRT1-U-00-12401	VCSIP_S_61	Sample Type:Study Sample | Batch:1 | Analysis Order:84	RAW_FILE_NAME=VCSIP_S_61
SUBJECT_SAMPLE_FACTORS           	EM-5E6L2-U-00-12401	VCSIP_S_62	Sample Type:Study Sample | Batch:1 | Analysis Order:85	RAW_FILE_NAME=VCSIP_S_62
SUBJECT_SAMPLE_FACTORS           	EM-6GJW3-U-00-12401	VCSIP_S_63	Sample Type:Study Sample | Batch:1 | Analysis Order:86	RAW_FILE_NAME=VCSIP_S_63
SUBJECT_SAMPLE_FACTORS           	EM-5DU79-U-00-12401	VCSIP_S_64	Sample Type:Study Sample | Batch:1 | Analysis Order:87	RAW_FILE_NAME=VCSIP_S_64
SUBJECT_SAMPLE_FACTORS           	EM-5DZU8-U-00-12401	VCSIP_S_65	Sample Type:Study Sample | Batch:1 | Analysis Order:88	RAW_FILE_NAME=VCSIP_S_65
SUBJECT_SAMPLE_FACTORS           	EM-6GBL5-U-00-12401	VCSIP_S_66	Sample Type:Study Sample | Batch:1 | Analysis Order:89	RAW_FILE_NAME=VCSIP_S_66
SUBJECT_SAMPLE_FACTORS           	EM-6GRA3-U-00-12401	VCSIP_S_8	Sample Type:Study Sample | Batch:1 | Analysis Order:9	RAW_FILE_NAME=VCSIP_S_8
SUBJECT_SAMPLE_FACTORS           	EM-5DU12-U-00-12401	VCSIP_S_67	Sample Type:Study Sample | Batch:1 | Analysis Order:90	RAW_FILE_NAME=VCSIP_S_67
SUBJECT_SAMPLE_FACTORS           	EM-6GHN4-U-00-12401	VCSIP_S_68	Sample Type:Study Sample | Batch:1 | Analysis Order:91	RAW_FILE_NAME=VCSIP_S_68
SUBJECT_SAMPLE_FACTORS           	EM-6GFB3-U-00-12401	VCSIP_S_69	Sample Type:Study Sample | Batch:1 | Analysis Order:92	RAW_FILE_NAME=VCSIP_S_69
SUBJECT_SAMPLE_FACTORS           	EM-5DKY4-U-00-12401	VCSIP_S_70	Sample Type:Study Sample | Batch:1 | Analysis Order:93	RAW_FILE_NAME=VCSIP_S_70
SUBJECT_SAMPLE_FACTORS           	EM-6GQQ9-U-00-12401	VCSIP_S_71	Sample Type:Study Sample | Batch:1 | Analysis Order:98	RAW_FILE_NAME=VCSIP_S_71
SUBJECT_SAMPLE_FACTORS           	EM-5DXW6-U-00-12401	VCSIP_S_72	Sample Type:Study Sample | Batch:1 | Analysis Order:99	RAW_FILE_NAME=VCSIP_S_72
SUBJECT_SAMPLE_FACTORS           	EM-6GND0-U-00-12401	VCSIP_S_81	Sample Type:Study Sample | Batch:2 | Analysis Order:113	RAW_FILE_NAME=VCSIP_S_81
SUBJECT_SAMPLE_FACTORS           	EM-5DQB9-U-00-12401	VCSIP_S_82	Sample Type:Study Sample | Batch:2 | Analysis Order:114	RAW_FILE_NAME=VCSIP_S_82
SUBJECT_SAMPLE_FACTORS           	EM-5DRQ5-U-00-12401	VCSIP_S_83	Sample Type:Study Sample | Batch:2 | Analysis Order:115	RAW_FILE_NAME=VCSIP_S_83
SUBJECT_SAMPLE_FACTORS           	EM-6G970-U-00-12401	VCSIP_S_84	Sample Type:Study Sample | Batch:2 | Analysis Order:116	RAW_FILE_NAME=VCSIP_S_84
SUBJECT_SAMPLE_FACTORS           	EM-6GG68-U-00-12401	VCSIP_S_85	Sample Type:Study Sample | Batch:2 | Analysis Order:117	RAW_FILE_NAME=VCSIP_S_85
SUBJECT_SAMPLE_FACTORS           	EM-6GLF8-U-00-12401	VCSIP_S_86	Sample Type:Study Sample | Batch:2 | Analysis Order:118	RAW_FILE_NAME=VCSIP_S_86
SUBJECT_SAMPLE_FACTORS           	EM-6GCP5-U-00-12401	VCSIP_S_87	Sample Type:Study Sample | Batch:2 | Analysis Order:119	RAW_FILE_NAME=VCSIP_S_87
SUBJECT_SAMPLE_FACTORS           	EM-5DQQ6-U-00-12401	VCSIP_S_88	Sample Type:Study Sample | Batch:2 | Analysis Order:120	RAW_FILE_NAME=VCSIP_S_88
SUBJECT_SAMPLE_FACTORS           	EM-5DUD0-U-00-12401	VCSIP_S_89	Sample Type:Study Sample | Batch:2 | Analysis Order:121	RAW_FILE_NAME=VCSIP_S_89
SUBJECT_SAMPLE_FACTORS           	EM-6GTD4-U-00-12401	VCSIP_S_90	Sample Type:Study Sample | Batch:2 | Analysis Order:122	RAW_FILE_NAME=VCSIP_S_90
SUBJECT_SAMPLE_FACTORS           	EM-6GE52-U-00-12401	VCSIP_S_91	Sample Type:Study Sample | Batch:2 | Analysis Order:127	RAW_FILE_NAME=VCSIP_S_91
SUBJECT_SAMPLE_FACTORS           	EM-6GPV9-U-00-12401	VCSIP_S_92	Sample Type:Study Sample | Batch:2 | Analysis Order:128	RAW_FILE_NAME=VCSIP_S_92
SUBJECT_SAMPLE_FACTORS           	EM-6GQC0-U-00-12401	VCSIP_S_93	Sample Type:Study Sample | Batch:2 | Analysis Order:129	RAW_FILE_NAME=VCSIP_S_93
SUBJECT_SAMPLE_FACTORS           	EM-5DGE2-U-00-12401	VCSIP_S_94	Sample Type:Study Sample | Batch:2 | Analysis Order:130	RAW_FILE_NAME=VCSIP_S_94
SUBJECT_SAMPLE_FACTORS           	EM-6GMV2-U-00-12401	VCSIP_S_95	Sample Type:Study Sample | Batch:2 | Analysis Order:131	RAW_FILE_NAME=VCSIP_S_95
SUBJECT_SAMPLE_FACTORS           	EM-6GHB1-U-00-12401	VCSIP_S_96	Sample Type:Study Sample | Batch:2 | Analysis Order:132	RAW_FILE_NAME=VCSIP_S_96
SUBJECT_SAMPLE_FACTORS           	EM-6GLV3-U-00-12401	VCSIP_S_97	Sample Type:Study Sample | Batch:2 | Analysis Order:133	RAW_FILE_NAME=VCSIP_S_97
SUBJECT_SAMPLE_FACTORS           	EM-5E6H1-U-00-12401	VCSIP_S_98	Sample Type:Study Sample | Batch:2 | Analysis Order:134	RAW_FILE_NAME=VCSIP_S_98
SUBJECT_SAMPLE_FACTORS           	EM-6GSP9-U-00-12401	VCSIP_S_99	Sample Type:Study Sample | Batch:2 | Analysis Order:135	RAW_FILE_NAME=VCSIP_S_99
SUBJECT_SAMPLE_FACTORS           	EM-5DXT2-U-00-12401	VCSIP_S_100	Sample Type:Study Sample | Batch:2 | Analysis Order:136	RAW_FILE_NAME=VCSIP_S_100
SUBJECT_SAMPLE_FACTORS           	EM-6GEJ6-U-00-12401	VCSIP_S_101	Sample Type:Study Sample | Batch:2 | Analysis Order:140	RAW_FILE_NAME=VCSIP_S_101
SUBJECT_SAMPLE_FACTORS           	EM-5E439-U-00-12401	VCSIP_S_102	Sample Type:Study Sample | Batch:2 | Analysis Order:141	RAW_FILE_NAME=VCSIP_S_102
SUBJECT_SAMPLE_FACTORS           	EM-6GUV4-U-00-12401	VCSIP_S_103	Sample Type:Study Sample | Batch:2 | Analysis Order:142	RAW_FILE_NAME=VCSIP_S_103
SUBJECT_SAMPLE_FACTORS           	EM-5DV37-U-00-12401	VCSIP_S_104	Sample Type:Study Sample | Batch:2 | Analysis Order:143	RAW_FILE_NAME=VCSIP_S_104
SUBJECT_SAMPLE_FACTORS           	EM-6GPQ0-U-00-12401	VCSIP_S_105	Sample Type:Study Sample | Batch:2 | Analysis Order:144	RAW_FILE_NAME=VCSIP_S_105
SUBJECT_SAMPLE_FACTORS           	EM-6GWM2-U-00-12401	VCSIP_S_106	Sample Type:Study Sample | Batch:2 | Analysis Order:145	RAW_FILE_NAME=VCSIP_S_106
SUBJECT_SAMPLE_FACTORS           	EM-5DYA3-U-00-12401	VCSIP_S_107	Sample Type:Study Sample | Batch:2 | Analysis Order:146	RAW_FILE_NAME=VCSIP_S_107
SUBJECT_SAMPLE_FACTORS           	EM-6GQY1-U-00-12401	VCSIP_S_108	Sample Type:Study Sample | Batch:2 | Analysis Order:147	RAW_FILE_NAME=VCSIP_S_108
SUBJECT_SAMPLE_FACTORS           	EM-6GBP6-U-00-12401	VCSIP_S_109	Sample Type:Study Sample | Batch:2 | Analysis Order:148	RAW_FILE_NAME=VCSIP_S_109
SUBJECT_SAMPLE_FACTORS           	EM-6GPK3-U-00-12401	VCSIP_S_110	Sample Type:Study Sample | Batch:2 | Analysis Order:149	RAW_FILE_NAME=VCSIP_S_110
SUBJECT_SAMPLE_FACTORS           	EM-6G889-U-00-12401	VCSIP_S_111	Sample Type:Study Sample | Batch:2 | Analysis Order:154	RAW_FILE_NAME=VCSIP_S_111
SUBJECT_SAMPLE_FACTORS           	EM-6GMN9-U-00-12401	VCSIP_S_112	Sample Type:Study Sample | Batch:2 | Analysis Order:155	RAW_FILE_NAME=VCSIP_S_112
SUBJECT_SAMPLE_FACTORS           	EM-6GHD6-U-00-12401	VCSIP_S_113	Sample Type:Study Sample | Batch:2 | Analysis Order:156	RAW_FILE_NAME=VCSIP_S_113
SUBJECT_SAMPLE_FACTORS           	EM-5E4S8-U-00-12401	VCSIP_S_114	Sample Type:Study Sample | Batch:2 | Analysis Order:157	RAW_FILE_NAME=VCSIP_S_114
SUBJECT_SAMPLE_FACTORS           	EM-5DP25-U-00-12401	VCSIP_S_115	Sample Type:Study Sample | Batch:2 | Analysis Order:158	RAW_FILE_NAME=VCSIP_S_115
SUBJECT_SAMPLE_FACTORS           	EM-5DHN1-U-00-12401	VCSIP_S_116	Sample Type:Study Sample | Batch:2 | Analysis Order:159	RAW_FILE_NAME=VCSIP_S_116
SUBJECT_SAMPLE_FACTORS           	EM-6GTU7-U-00-12401	VCSIP_S_117	Sample Type:Study Sample | Batch:2 | Analysis Order:160	RAW_FILE_NAME=VCSIP_S_117
SUBJECT_SAMPLE_FACTORS           	EM-5E2L6-U-00-12401	VCSIP_S_118	Sample Type:Study Sample | Batch:2 | Analysis Order:161	RAW_FILE_NAME=VCSIP_S_118
SUBJECT_SAMPLE_FACTORS           	EM-5DTG5-U-00-12401	VCSIP_S_119	Sample Type:Study Sample | Batch:2 | Analysis Order:162	RAW_FILE_NAME=VCSIP_S_119
SUBJECT_SAMPLE_FACTORS           	EM-6GD46-U-00-12401	VCSIP_S_120	Sample Type:Study Sample | Batch:2 | Analysis Order:163	RAW_FILE_NAME=VCSIP_S_120
SUBJECT_SAMPLE_FACTORS           	EM-5DW10-U-00-12401	VCSIP_S_121	Sample Type:Study Sample | Batch:2 | Analysis Order:168	RAW_FILE_NAME=VCSIP_S_121
SUBJECT_SAMPLE_FACTORS           	EM-6GF36-U-00-12401	VCSIP_S_122	Sample Type:Study Sample | Batch:2 | Analysis Order:169	RAW_FILE_NAME=VCSIP_S_122
SUBJECT_SAMPLE_FACTORS           	EM-5DQA1-U-00-12401	VCSIP_S_123	Sample Type:Study Sample | Batch:2 | Analysis Order:170	RAW_FILE_NAME=VCSIP_S_123
SUBJECT_SAMPLE_FACTORS           	EM-6GWP5-U-00-12401	VCSIP_S_124	Sample Type:Study Sample | Batch:2 | Analysis Order:171	RAW_FILE_NAME=VCSIP_S_124
SUBJECT_SAMPLE_FACTORS           	EM-5DY42-U-00-12401	VCSIP_S_125	Sample Type:Study Sample | Batch:2 | Analysis Order:172	RAW_FILE_NAME=VCSIP_S_125
SUBJECT_SAMPLE_FACTORS           	EM-6GL71-U-00-12401	VCSIP_S_126	Sample Type:Study Sample | Batch:2 | Analysis Order:173	RAW_FILE_NAME=VCSIP_S_126
SUBJECT_SAMPLE_FACTORS           	EM-5E4V2-U-00-12401	VCSIP_S_127	Sample Type:Study Sample | Batch:2 | Analysis Order:174	RAW_FILE_NAME=VCSIP_S_127
SUBJECT_SAMPLE_FACTORS           	EM-6GVX8-U-00-12401	VCSIP_S_128	Sample Type:Study Sample | Batch:2 | Analysis Order:175	RAW_FILE_NAME=VCSIP_S_128
SUBJECT_SAMPLE_FACTORS           	EM-5E6V0-U-00-12401	VCSIP_S_129	Sample Type:Study Sample | Batch:2 | Analysis Order:176	RAW_FILE_NAME=VCSIP_S_129
SUBJECT_SAMPLE_FACTORS           	EM-5DNC0-U-00-12401	VCSIP_S_130	Sample Type:Study Sample | Batch:2 | Analysis Order:177	RAW_FILE_NAME=VCSIP_S_130
SUBJECT_SAMPLE_FACTORS           	EM-5DJX7-U-00-12401	VCSIP_S_131	Sample Type:Study Sample | Batch:2 | Analysis Order:182	RAW_FILE_NAME=VCSIP_S_131
SUBJECT_SAMPLE_FACTORS           	EM-5DD50-U-00-12401	VCSIP_S_132	Sample Type:Study Sample | Batch:2 | Analysis Order:183	RAW_FILE_NAME=VCSIP_S_132
SUBJECT_SAMPLE_FACTORS           	EM-6GJF0-U-00-12401	VCSIP_S_133	Sample Type:Study Sample | Batch:2 | Analysis Order:184	RAW_FILE_NAME=VCSIP_S_133
SUBJECT_SAMPLE_FACTORS           	EM-6GEB4-U-00-12401	VCSIP_S_134	Sample Type:Study Sample | Batch:2 | Analysis Order:185	RAW_FILE_NAME=VCSIP_S_134
SUBJECT_SAMPLE_FACTORS           	EM-6GQS4-U-00-12401	VCSIP_S_135	Sample Type:Study Sample | Batch:2 | Analysis Order:186	RAW_FILE_NAME=VCSIP_S_135
SUBJECT_SAMPLE_FACTORS           	EM-5E3S9-U-00-12401	VCSIP_S_136	Sample Type:Study Sample | Batch:2 | Analysis Order:187	RAW_FILE_NAME=VCSIP_S_136
SUBJECT_SAMPLE_FACTORS           	EM-5DFH7-U-00-12401	VCSIP_S_137	Sample Type:Study Sample | Batch:2 | Analysis Order:188	RAW_FILE_NAME=VCSIP_S_137
SUBJECT_SAMPLE_FACTORS           	EM-5DWL1-U-00-12401	VCSIP_S_138	Sample Type:Study Sample | Batch:2 | Analysis Order:189	RAW_FILE_NAME=VCSIP_S_138
SUBJECT_SAMPLE_FACTORS           	EM-5DZ25-U-00-12401	VCSIP_S_139	Sample Type:Study Sample | Batch:2 | Analysis Order:190	RAW_FILE_NAME=VCSIP_S_139
SUBJECT_SAMPLE_FACTORS           	EM-6G9P5-U-00-12401	VCSIP_S_140	Sample Type:Study Sample | Batch:2 | Analysis Order:191	RAW_FILE_NAME=VCSIP_S_140
SUBJECT_SAMPLE_FACTORS           	EM-6GJ81-U-00-12401	VCSIP_S_141	Sample Type:Study Sample | Batch:2 | Analysis Order:195	RAW_FILE_NAME=VCSIP_S_141
SUBJECT_SAMPLE_FACTORS           	EM-6GP69-U-00-12401	VCSIP_S_142	Sample Type:Study Sample | Batch:2 | Analysis Order:196	RAW_FILE_NAME=VCSIP_S_142
SUBJECT_SAMPLE_FACTORS           	EM-6GDT5-U-00-12401	VCSIP_S_143	Sample Type:Study Sample | Batch:2 | Analysis Order:197	RAW_FILE_NAME=VCSIP_S_143
SUBJECT_SAMPLE_FACTORS           	EM-5DVM0-U-00-12401	VCSIP_S_144	Sample Type:Study Sample | Batch:2 | Analysis Order:198	RAW_FILE_NAME=VCSIP_S_144
SUBJECT_SAMPLE_FACTORS           	EM-6GN61-U-00-12401	VCSIP_S_145	Sample Type:Study Sample | Batch:2 | Analysis Order:199	RAW_FILE_NAME=VCSIP_S_145
SUBJECT_SAMPLE_FACTORS           	EM-5DTK6-U-00-12401	VCSIP_S_146	Sample Type:Study Sample | Batch:2 | Analysis Order:200	RAW_FILE_NAME=VCSIP_S_146
SUBJECT_SAMPLE_FACTORS           	EM-5DRN1-U-00-12401	VCSIP_S_147	Sample Type:Study Sample | Batch:2 | Analysis Order:201	RAW_FILE_NAME=VCSIP_S_147
SUBJECT_SAMPLE_FACTORS           	EM-5E330-U-00-12401	VCSIP_S_148	Sample Type:Study Sample | Batch:2 | Analysis Order:202	RAW_FILE_NAME=VCSIP_S_148
SUBJECT_SAMPLE_FACTORS           	EM-5DWC1-U-00-12401	VCSIP_S_149	Sample Type:Study Sample | Batch:2 | Analysis Order:203	RAW_FILE_NAME=VCSIP_S_149
SUBJECT_SAMPLE_FACTORS           	EM-5DPB0-U-00-12401	VCSIP_S_150	Sample Type:Study Sample | Batch:2 | Analysis Order:204	RAW_FILE_NAME=VCSIP_S_150
SUBJECT_SAMPLE_FACTORS           	EM-5DPL8-U-00-12401	VCSIP_S_151	Sample Type:Study Sample | Batch:2 | Analysis Order:209	RAW_FILE_NAME=VCSIP_S_151
SUBJECT_SAMPLE_FACTORS           	EM-6GNR0-U-00-12401	VCSIP_S_152	Sample Type:Study Sample | Batch:2 | Analysis Order:210	RAW_FILE_NAME=VCSIP_S_152
SUBJECT_SAMPLE_FACTORS           	EM-6GRB1-U-00-12401	VCSIP_S_153	Sample Type:Study Sample | Batch:2 | Analysis Order:211	RAW_FILE_NAME=VCSIP_S_153
SUBJECT_SAMPLE_FACTORS           	EM-6GUF9-U-00-12401	VCSIP_S_154	Sample Type:Study Sample | Batch:2 | Analysis Order:212	RAW_FILE_NAME=VCSIP_S_154
SUBJECT_SAMPLE_FACTORS           	EM-6GUY7-U-00-12401	VCSIP_S_155	Sample Type:Study Sample | Batch:2 | Analysis Order:213	RAW_FILE_NAME=VCSIP_S_155
SUBJECT_SAMPLE_FACTORS           	EM-5DMU1-U-00-12401	VCSIP_S_156	Sample Type:Study Sample | Batch:2 | Analysis Order:214	RAW_FILE_NAME=VCSIP_S_156
SUBJECT_SAMPLE_FACTORS           	EM-5DTP5-U-00-12401	VCSIP_S_157	Sample Type:Study Sample | Batch:2 | Analysis Order:215	RAW_FILE_NAME=VCSIP_S_157
SUBJECT_SAMPLE_FACTORS           	EM-5DQ99-U-00-12401	VCSIP_S_158	Sample Type:Study Sample | Batch:2 | Analysis Order:216	RAW_FILE_NAME=VCSIP_S_158
SUBJECT_SAMPLE_FACTORS           	EM-5DKK5-U-00-12401	VCSIP_S_159	Sample Type:Study Sample | Batch:2 | Analysis Order:217	RAW_FILE_NAME=VCSIP_S_159
SUBJECT_SAMPLE_FACTORS           	EM-5DEG0-U-00-12401	VCSIP_S_160	Sample Type:Study Sample | Batch:2 | Analysis Order:218	RAW_FILE_NAME=VCSIP_S_160
SUBJECT_SAMPLE_FACTORS           	EM-5DZ33-U-00-12401	VCSIP_S_161	Sample Type:Study Sample | Batch:3 | Analysis Order:224	RAW_FILE_NAME=VCSIP_S_161
SUBJECT_SAMPLE_FACTORS           	EM-6GLQ4-U-00-12401	VCSIP_S_162	Sample Type:Study Sample | Batch:3 | Analysis Order:225	RAW_FILE_NAME=VCSIP_S_162
SUBJECT_SAMPLE_FACTORS           	EM-5DNP1-U-00-12401	VCSIP_S_163	Sample Type:Study Sample | Batch:3 | Analysis Order:226	RAW_FILE_NAME=VCSIP_S_163
SUBJECT_SAMPLE_FACTORS           	EM-6G8M3-U-00-12401	VCSIP_S_164	Sample Type:Study Sample | Batch:3 | Analysis Order:227	RAW_FILE_NAME=VCSIP_S_164
SUBJECT_SAMPLE_FACTORS           	EM-5E5Q2-U-00-12401	VCSIP_S_165	Sample Type:Study Sample | Batch:3 | Analysis Order:228	RAW_FILE_NAME=VCSIP_S_165
SUBJECT_SAMPLE_FACTORS           	EM-6GM39-U-00-12401	VCSIP_S_166	Sample Type:Study Sample | Batch:3 | Analysis Order:229	RAW_FILE_NAME=VCSIP_S_166
SUBJECT_SAMPLE_FACTORS           	EM-5E520-U-00-12401	VCSIP_S_167	Sample Type:Study Sample | Batch:3 | Analysis Order:230	RAW_FILE_NAME=VCSIP_S_167
SUBJECT_SAMPLE_FACTORS           	EM-5DLM0-U-00-12401	VCSIP_S_168	Sample Type:Study Sample | Batch:3 | Analysis Order:231	RAW_FILE_NAME=VCSIP_S_168
SUBJECT_SAMPLE_FACTORS           	EM-5DGM5-U-00-12401	VCSIP_S_169	Sample Type:Study Sample | Batch:3 | Analysis Order:232	RAW_FILE_NAME=VCSIP_S_169
SUBJECT_SAMPLE_FACTORS           	EM-5DF58-U-00-12401	VCSIP_S_170	Sample Type:Study Sample | Batch:3 | Analysis Order:233	RAW_FILE_NAME=VCSIP_S_170
SUBJECT_SAMPLE_FACTORS           	EM-5DZT0-U-00-12401	VCSIP_S_171	Sample Type:Study Sample | Batch:3 | Analysis Order:238	RAW_FILE_NAME=VCSIP_S_171
SUBJECT_SAMPLE_FACTORS           	EM-5DE91-U-00-12401	VCSIP_S_172	Sample Type:Study Sample | Batch:3 | Analysis Order:239	RAW_FILE_NAME=VCSIP_S_172
SUBJECT_SAMPLE_FACTORS           	EM-5DDH9-U-00-12401	VCSIP_S_173	Sample Type:Study Sample | Batch:3 | Analysis Order:240	RAW_FILE_NAME=VCSIP_S_173
SUBJECT_SAMPLE_FACTORS           	EM-5E4J8-U-00-12401	VCSIP_S_174	Sample Type:Study Sample | Batch:3 | Analysis Order:241	RAW_FILE_NAME=VCSIP_S_174
SUBJECT_SAMPLE_FACTORS           	EM-5DL52-U-00-12401	VCSIP_S_175	Sample Type:Study Sample | Batch:3 | Analysis Order:242	RAW_FILE_NAME=VCSIP_S_175
SUBJECT_SAMPLE_FACTORS           	EM-6GUG7-U-00-12401	VCSIP_S_176	Sample Type:Study Sample | Batch:3 | Analysis Order:243	RAW_FILE_NAME=VCSIP_S_176
SUBJECT_SAMPLE_FACTORS           	EM-5E1T9-U-00-12401	VCSIP_S_177	Sample Type:Study Sample | Batch:3 | Analysis Order:244	RAW_FILE_NAME=VCSIP_S_177
SUBJECT_SAMPLE_FACTORS           	EM-5DTD1-U-00-12401	VCSIP_S_178	Sample Type:Study Sample | Batch:3 | Analysis Order:245	RAW_FILE_NAME=VCSIP_S_178
SUBJECT_SAMPLE_FACTORS           	EM-6GWT6-U-00-12401	VCSIP_S_179	Sample Type:Study Sample | Batch:3 | Analysis Order:246	RAW_FILE_NAME=VCSIP_S_179
SUBJECT_SAMPLE_FACTORS           	EM-5DDA4-U-00-12401	VCSIP_S_180	Sample Type:Study Sample | Batch:3 | Analysis Order:247	RAW_FILE_NAME=VCSIP_S_180
SUBJECT_SAMPLE_FACTORS           	EM-6GGD7-U-00-12401	VCSIP_S_181	Sample Type:Study Sample | Batch:3 | Analysis Order:252	RAW_FILE_NAME=VCSIP_S_181
SUBJECT_SAMPLE_FACTORS           	EM-6G897-U-00-12401	VCSIP_S_182	Sample Type:Study Sample | Batch:3 | Analysis Order:253	RAW_FILE_NAME=VCSIP_S_182
SUBJECT_SAMPLE_FACTORS           	EM-5DSH4-U-00-12401	VCSIP_S_183	Sample Type:Study Sample | Batch:3 | Analysis Order:254	RAW_FILE_NAME=VCSIP_S_183
SUBJECT_SAMPLE_FACTORS           	EM-5DDT2-U-00-12401	VCSIP_S_184	Sample Type:Study Sample | Batch:3 | Analysis Order:255	RAW_FILE_NAME=VCSIP_S_184
SUBJECT_SAMPLE_FACTORS           	EM-6GBR2-U-00-12401	VCSIP_S_185	Sample Type:Study Sample | Batch:3 | Analysis Order:256	RAW_FILE_NAME=VCSIP_S_185
SUBJECT_SAMPLE_FACTORS           	EM-5E1C7-U-00-12401	VCSIP_S_186	Sample Type:Study Sample | Batch:3 | Analysis Order:257	RAW_FILE_NAME=VCSIP_S_186
SUBJECT_SAMPLE_FACTORS           	EM-6G9M2-U-00-12401	VCSIP_S_187	Sample Type:Study Sample | Batch:3 | Analysis Order:258	RAW_FILE_NAME=VCSIP_S_187
SUBJECT_SAMPLE_FACTORS           	EM-6GS09-U-00-12401	VCSIP_S_188	Sample Type:Study Sample | Batch:3 | Analysis Order:259	RAW_FILE_NAME=VCSIP_S_188
SUBJECT_SAMPLE_FACTORS           	EM-5DP33-U-00-12401	VCSIP_S_189	Sample Type:Study Sample | Batch:3 | Analysis Order:260	RAW_FILE_NAME=VCSIP_S_189
SUBJECT_SAMPLE_FACTORS           	EM-6GP85-U-00-12401	VCSIP_S_190	Sample Type:Study Sample | Batch:3 | Analysis Order:261	RAW_FILE_NAME=VCSIP_S_190
SUBJECT_SAMPLE_FACTORS           	EM-5DZ58-U-00-12401	VCSIP_S_191	Sample Type:Study Sample | Batch:3 | Analysis Order:266	RAW_FILE_NAME=VCSIP_S_191
SUBJECT_SAMPLE_FACTORS           	EM-5DF09-U-00-12401	VCSIP_S_192	Sample Type:Study Sample | Batch:3 | Analysis Order:267	RAW_FILE_NAME=VCSIP_S_192
SUBJECT_SAMPLE_FACTORS           	EM-6GNX6-U-00-12401	VCSIP_S_193	Sample Type:Study Sample | Batch:3 | Analysis Order:268	RAW_FILE_NAME=VCSIP_S_193
SUBJECT_SAMPLE_FACTORS           	EM-6GBA9-U-00-12401	VCSIP_S_194	Sample Type:Study Sample | Batch:3 | Analysis Order:269	RAW_FILE_NAME=VCSIP_S_194
SUBJECT_SAMPLE_FACTORS           	EM-5DF66-U-00-12401	VCSIP_S_195	Sample Type:Study Sample | Batch:3 | Analysis Order:270	RAW_FILE_NAME=VCSIP_S_195
SUBJECT_SAMPLE_FACTORS           	EM-5DW77-U-00-12401	VCSIP_S_196	Sample Type:Study Sample | Batch:3 | Analysis Order:271	RAW_FILE_NAME=VCSIP_S_196
SUBJECT_SAMPLE_FACTORS           	EM-5DN19-U-00-12401	VCSIP_S_197	Sample Type:Study Sample | Batch:3 | Analysis Order:272	RAW_FILE_NAME=VCSIP_S_197
SUBJECT_SAMPLE_FACTORS           	EM-5DSA9-U-00-12401	VCSIP_S_198	Sample Type:Study Sample | Batch:3 | Analysis Order:273	RAW_FILE_NAME=VCSIP_S_198
SUBJECT_SAMPLE_FACTORS           	EM-6G9Y5-U-00-12401	VCSIP_S_199	Sample Type:Study Sample | Batch:3 | Analysis Order:274	RAW_FILE_NAME=VCSIP_S_199
SUBJECT_SAMPLE_FACTORS           	EM-6GFW7-U-00-12401	VCSIP_S_200	Sample Type:Study Sample | Batch:3 | Analysis Order:275	RAW_FILE_NAME=VCSIP_S_200
#COLLECTION
CO:COLLECTION_SUMMARY            	N/A
CO:SAMPLE_TYPE                   	Urine
CO:STORAGE_CONDITIONS            	-80℃
#TREATMENT
TR:TREATMENT_SUMMARY             	NA
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	Study samples were shipped from the Oregon Health and Sciences University to the
SP:SAMPLEPREP_SUMMARY            	NC HHEAR Hub on dry ice. The NC HHEAR Hub thawed and transferred 50 µL of the
SP:SAMPLEPREP_SUMMARY            	study samples to a new set of tubes and used them for the analysis. An
SP:SAMPLEPREP_SUMMARY            	additional 10 µL was taken from the original study sample and transferred to
SP:SAMPLEPREP_SUMMARY            	another tube to make the total study pool for this project, and then distributed
SP:SAMPLEPREP_SUMMARY            	with 50 µL per aliquot, used as quality control study pools (QC study pools)
SP:SAMPLEPREP_SUMMARY            	throughout the whole analysis. All sample aliquots (50 µL each) and QC study
SP:SAMPLEPREP_SUMMARY            	pools (50 µL each) were stored at -80° C until the day of sample preparation.
SP:SAMPLEPREP_SUMMARY            	HHEAR Urine Pool 1 (50 µL each), HHEAR Urine Pool 2 (50 µL each), CHEAR
SP:SAMPLEPREP_SUMMARY            	reference urine (50 µL each) and NIST urine (SRM 3672) reference material (50
SP:SAMPLEPREP_SUMMARY            	µL each) were provided by the NC HHEAR Hub. LC-MS grade water (50 µL) was used
SP:SAMPLEPREP_SUMMARY            	as blanks. All samples were thawed at 4°C overnight before the preparation.
SP:SAMPLEPREP_SUMMARY            	Samples, including study samples, study pool samples, HHEAR reference urine,
SP:SAMPLEPREP_SUMMARY            	CHEAR reference urine, NIST reference urine, and blanks were mixed with 400 µL
SP:SAMPLEPREP_SUMMARY            	methanol containing 500 ng/mL tryptophan-d5 as internal standard and vortexed by
SP:SAMPLEPREP_SUMMARY            	a multiple tube vortex mixer for 2 min at 5000 rpm at room temperature. All
SP:SAMPLEPREP_SUMMARY            	samples were centrifuged at 16,000 rcf for 10 min at 4°C. The supernatant (350
SP:SAMPLEPREP_SUMMARY            	µL) was transferred into a pre-labeled 2.0 mL Lo-bind Eppendorf tube, dried by
SP:SAMPLEPREP_SUMMARY            	a SpeedVac overnight, and stored at -80° C. For immediate analysis, 100 µL of
SP:SAMPLEPREP_SUMMARY            	water-methanol solution (95:5, v/v) was used to reconstitute the dried extracts.
SP:SAMPLEPREP_SUMMARY            	Samples were thoroughly mixed on a multiple tube vortex mixer for 10 min at 5000
SP:SAMPLEPREP_SUMMARY            	rpm at room temperature and then centrifuged at 4°C for 10 min at 16,000 rcf.
SP:SAMPLEPREP_SUMMARY            	The supernatant was transferred to pre-labeled autosampler vials for data
SP:SAMPLEPREP_SUMMARY            	acquisition by LC-MS.
SP:PROCESSING_STORAGE_CONDITIONS 	4℃
SP:EXTRACTION_METHOD             	Vortex with methanol containing 500ng/ml tryptophan-d5 as internal standard
SP:EXTRACT_STORAGE               	-80℃
SP:SAMPLE_RESUSPENSION           	Water-Methanol (95:5, v/v)
SP:SAMPLE_SPIKING                	Tryptophan-d5 stock solution at 500 ng/mL
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_SUMMARY        	Reverse phase
CH:INSTRUMENT_NAME               	Thermo Vanquish
CH:COLUMN_NAME                   	Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,1.8um)
CH:COLUMN_PRESSURE               	6000-10000 psi
CH:COLUMN_TEMPERATURE            	50℃
CH:FLOW_GRADIENT                 	Time(min) Flow Rate %A %B Curve 1. 0 0.4 99.0 1.0 5 2. 1.00 0.4 99.0 1.0 5 3.
CH:FLOW_GRADIENT                 	16.00 0.4 1.0 99.0 5 4. 19.00 0.4 1.0 99.0 5 5. 19.50 0.4 99.0 1.0 5 6. 22.00
CH:FLOW_GRADIENT                 	0.4 99.0 1.0 5
CH:FLOW_RATE                     	0.4 mL/min
CH:INJECTION_TEMPERATURE         	8oC
CH:INTERNAL_STANDARD             	Tryptophan-d5
CH:SOLVENT_A                     	100% water; 0.1% formic acid
CH:SOLVENT_B                     	100% methanol; 0.1% formic acid
CH:ANALYTICAL_TIME               	22 min
CH:WEAK_WASH_SOLVENT_NAME        	10% methanol/90% water; 0.1% formic acid
CH:STRONG_WASH_SOLVENT_NAME      	75% 2-Propanol/25% Water; 0.1% formic acid
CH:CHROMATOGRAPHY_TYPE           	Reversed phase
#ANALYSIS
AN:LABORATORY_NAME               	Sumner Lab, UNC-CH
AN:ANALYSIS_TYPE                 	MS
AN:ACQUISITION_DATE              	12/7/2022-12/12/2022
AN:SOFTWARE_VERSION              	Xcalibur 4.1.31.9
AN:DETECTOR_TYPE                 	Orbitrap
AN:DATA_FORMAT                   	Profile
#MS
MS:INSTRUMENT_NAME               	Thermo Q Exactive HF-X Orbitrap
MS:INSTRUMENT_TYPE               	Orbitrap
MS:MS_TYPE                       	ESI
MS:MS_COMMENTS                   	Instrument: Thermo Q Exactive HFx Software: Xcalibur 4.1.31.9 for data
MS:MS_COMMENTS                   	acquisition; Progenesis QI 2.4 for data preprocessing
MS:ION_MODE                      	POSITIVE
MS:CAPILLARY_TEMPERATURE         	320 °C
MS:CAPILLARY_VOLTAGE             	3.5 KV
MS:COLLISION_ENERGY              	20-45, ramp
MS:COLLISION_GAS                 	N2
MS:DRY_GAS_FLOW                  	55
MS:DRY_GAS_TEMP                  	400°C
MS:FRAGMENTATION_METHOD          	CID
MS:ION_SPRAY_VOLTAGE             	3.5kV
MS:IONIZATION                    	ES+
MS:MASS_ACCURACY                 	5 ppm
MS:DATAFORMAT                    	Profile
MS:DESOLVATION_GAS_FLOW          	55
MS:DESOLVATION_TEMPERATURE       	400 oC
MS:MS_RESULTS_FILE               	ST002559_AN004218_Results.txt	UNITS:m/z	Has m/z:Yes	Has RT:Yes	RT units:Minutes
#END