#METABOLOMICS WORKBENCH M_Sasaki_20230421_000842 DATATRACK_ID:3879 STUDY_ID:ST002584 ANALYSIS_ID:AN004256 PROJECT_ID:PR001664
VERSION             	1
CREATED_ON             	April 26, 2023, 11:57 am
#PROJECT
PR:PROJECT_TITLE                 	Metabolomics analysis of ALDH1L1-expressing HuH-7 cell line.
PR:PROJECT_SUMMARY               	Quantitative metabolomics study of the effect of the folate metabolizing enzyme
PR:PROJECT_SUMMARY               	ALDH1L1 on the HuH-7 cell line.
PR:INSTITUTE                     	Tohoku Medical and Pharmaceutical University
PR:LAST_NAME                     	Sasaki
PR:FIRST_NAME                    	Masato
PR:ADDRESS                       	4-4-1 Komatsushima, Aoba-ku, Sendai, Miyagi, 981-8558, Japan
PR:EMAIL                         	msasaki@tohoku-mpu.ac.jp
PR:PHONE                         	+81-22-727-0132
#STUDY
ST:STUDY_TITLE                   	Metabolomics analysis of ALDH1L1-expressing HuH7 cell lines.
ST:STUDY_SUMMARY                 	One-carbon metabolism is utilized for the de novo synthesis of purines. Given
ST:STUDY_SUMMARY                 	that tumor cells require large amounts of nucleotides, they are highly dependent
ST:STUDY_SUMMARY                 	on one-carbon metabolism. Among the one-carbon-metabolizing enzymes, the
ST:STUDY_SUMMARY                 	aldehyde dehydrogenase 1 family member L1 (ALDH1L1), which catalyzes
ST:STUDY_SUMMARY                 	10-formyltetrahydrofolate to trahydrofolate, is considered a tumor suppressor
ST:STUDY_SUMMARY                 	gene, as its expression is reported to be attenuated or diminished in
ST:STUDY_SUMMARY                 	hepatocellular carcinoma. To clarify the effect of ALDH1L1 expression, we
ST:STUDY_SUMMARY                 	generated control and ALDH1L1-expressing HuH-7 (H7-1L1) cells, and measured the
ST:STUDY_SUMMARY                 	amounts of intracellular metabolites in both control and ALDH1L1-expressing by
ST:STUDY_SUMMARY                 	CE-TOFMS analysis.
ST:INSTITUTE                     	Tohoku Medical and Pharmaceutical University
ST:LAST_NAME                     	Sasaki
ST:FIRST_NAME                    	Masato
ST:ADDRESS                       	4-4-1 Komatsushima, Aoba-ku, Sendai, Miyagi, 981-8558, Japan
ST:EMAIL                         	msasaki@tohoku-mpu.ac.jp
ST:PHONE                         	+81-22-727-0132
#SUBJECT
SU:SUBJECT_TYPE                  	Cultured cells
SU:SUBJECT_SPECIES               	Homo sapiens
SU:TAXONOMY_ID                   	9606
#FACTORS
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data
SUBJECT_SAMPLE_FACTORS           	L1-1	L1-1	Transgene:ALDH1L1	RAW_FILE_NAME=L1-1
SUBJECT_SAMPLE_FACTORS           	L1-2	L1-2	Transgene:ALDH1L1	RAW_FILE_NAME=L1-2
SUBJECT_SAMPLE_FACTORS           	L1-3	L1-3	Transgene:ALDH1L1	RAW_FILE_NAME=L1-3
SUBJECT_SAMPLE_FACTORS           	C-1	C-1	Transgene:empty	RAW_FILE_NAME=C-1
SUBJECT_SAMPLE_FACTORS           	C-2	C-2	Transgene:empty	RAW_FILE_NAME=C-2
SUBJECT_SAMPLE_FACTORS           	C-3	C-3	Transgene:empty	RAW_FILE_NAME=C-3
#COLLECTION
CO:COLLECTION_SUMMARY            	ALDH1L1-expressing cells or control (empty vector) cells were cultured in DMEM
CO:COLLECTION_SUMMARY            	medium containing 5% fetal bovine serum and metabolites were collected during
CO:COLLECTION_SUMMARY            	growing phase.
CO:SAMPLE_TYPE                   	HuH-7 cells
#TREATMENT
TR:TREATMENT_SUMMARY             	No treatment in this study.
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	Cells were washed with 5%(w/w) mannitol solution, and then extracted with
SP:SAMPLEPREP_SUMMARY            	methanol. Extracts were filtered through a 5-kDa cut-off filter
SP:SAMPLEPREP_SUMMARY            	(Ultrafree-MC-PLHCC, Human Metabolome Technologies) at 9,100 × g for 2 h at
SP:SAMPLEPREP_SUMMARY            	4 °C, dried in a centrifugal evaporator at 1,500 rpm at 1,000 Pa, and
SP:SAMPLEPREP_SUMMARY            	resuspended in 50 μl of ultrapure water before metabolome analysis.
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_SUMMARY        	CE-TOFMS (ESI negative)
CH:CHROMATOGRAPHY_TYPE           	CE
CH:INSTRUMENT_NAME               	Agilent CE
CH:COLUMN_NAME                   	Agilent Fused silica capillary (80cm x 50um)
CH:SOLVENT_A                     	50 mM ammonium acetate
CH:SOLVENT_B                     	-
CH:FLOW_GRADIENT                 	-
CH:FLOW_RATE                     	−30 kV
CH:COLUMN_TEMPERATURE            	20
#ANALYSIS
AN:ANALYSIS_TYPE                 	MS
#MS
MS:INSTRUMENT_NAME               	Agilent CE-TOFMS
MS:INSTRUMENT_TYPE               	Other
MS:MS_TYPE                       	ESI
MS:ION_MODE                      	NEGATIVE
MS:MS_COMMENTS                   	The spectrometer was scanned from m/z 50 to 1,000. Peaks were extracted using
MS:MS_COMMENTS                   	automatic integration software MasterHands (ver.2.16.0.15 Keio University).
MS:MS_COMMENTS                   	Signal peaks corresponding to isotopomers, adduct ions, and other product ions
MS:MS_COMMENTS                   	of known metabolites were excluded, and remaining peaks were annotated with
MS:MS_COMMENTS                   	putative metabolites from the HMT metabolite database based on their migration
MS:MS_COMMENTS                   	time and m/z values. Peak areas were normalized to internal standards and sample
MS:MS_COMMENTS                   	amounts.
#MS_METABOLITE_DATA
MS_METABOLITE_DATA:UNITS	Relative Area
MS_METABOLITE_DATA_START
Samples	L1-1	L1-2	L1-3	C-1	C-2	C-3
Factors	Transgene:ALDH1L1	Transgene:ALDH1L1	Transgene:ALDH1L1	Transgene:empty	Transgene:empty	Transgene:empty
Urea	2.4E-01	2.4E-01	1.8E-01	2.3E-01	1.9E-01	1.9E-01
Ethanolamine	N.D.	N.D.	N.D.	3.9E-03	3.2E-03	2.9E-03
Aminoacetone	1.9E-02	1.6E-02	1.9E-02	2.2E-02	2.0E-02	1.9E-02
Gly	5.6E+00	5.8E+00	5.6E+00	4.9E+00	5.0E+00	5.1E+00
Trimethylamine N-oxide	3.7E-03	3.8E-03	3.8E-03	3.9E-03	4.2E-03	3.5E-03
Putrescine	1.2E-02	1.0E-02	1.0E-02	1.2E-02	1.2E-02	1.1E-02
β-Ala	1.2E+00	1.3E+00	1.2E+00	1.2E+00	1.3E+00	1.3E+00
Ala	6.5E+00	7.0E+00	7.0E+00	6.4E+00	6.8E+00	6.8E+00
Glycerol	1.3E+00	1.8E+00	1.4E+00	1.3E+00	1.5E+00	1.6E+00
1-Aminocyclopropane-1-carboxylic acid / Homoserinelactone	7.0E-03	7.4E-03	6.3E-03	8.2E-03	6.2E-03	6.0E-03
3-Aminoisobutyric acid	1.3E-02	1.4E-02	2.0E-02	1.4E-02	1.9E-02	1.7E-02
2-Aminoisobutyric acid / 2-Aminobutyric acid	8.2E-02	7.8E-02	7.9E-02	8.3E-02	9.1E-02	8.3E-02
GABA	1.7E-01	2.0E-01	2.1E-01	2.3E-01	2.4E-01	2.5E-01
Choline	1.2E-01	1.2E-01	1.2E-01	1.2E-01	1.3E-01	1.2E-01
Ser	1.6E+00	1.6E+00	1.4E+00	2.3E+00	2.3E+00	2.3E+00
Diethanolamine	1.7E-02	1.7E-02	1.8E-02	3.3E-01	3.4E-02	2.9E-02
Hypotaurine	2.7E-01	3.0E-01	3.0E-01	2.5E-01	2.9E-01	2.6E-01
1-Pyrroline 5-carboxylic acid	5.4E-02	7.6E-02	7.5E-02	5.6E-02	8.4E-02	8.3E-02
Creatinine	2.9E-02	2.8E-02	2.7E-02	2.9E-02	2.8E-02	2.9E-02
Pro	4.0E-01	4.5E-01	4.3E-01	4.1E-01	4.3E-01	4.4E-01
5-Aminovaleric acid	1.9E-02	1.9E-02	1.8E-02	1.8E-02	1.9E-02	1.9E-02
Betaine	1.0E-01	1.2E-01	1.1E-01	1.1E-01	1.1E-01	1.1E-01
Val	4.8E+00	4.7E+00	4.1E+00	4.8E+00	4.5E+00	4.6E+00
Thr	7.1E+00	7.4E+00	7.0E+00	7.5E+00	7.5E+00	7.4E+00
Homoserine	4.2E-03	5.2E-03	N.D.	6.0E-03	5.3E-03	5.3E-03
Cys	1.4E-02	2.8E-02	8.4E-03	1.9E-02	8.0E-03	2.6E-02
Nicotinamide	3.8E-02	4.3E-02	4.8E-02	4.0E-02	3.4E-02	4.1E-02
Taurine	4.3E-02	4.0E-02	4.3E-02	4.1E-02	4.2E-02	4.2E-02
Imidazole-4-acetic acid	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
1-Aminocyclopentanecarboxylic acid	8.2E-03	8.3E-03	7.3E-03	7.5E-03	7.6E-03	7.4E-03
Pipecolic acid	2.4E-03	2.3E-03	2.2E-03	N.D.	2.3E-03	2.6E-03
trans-Glutaconic acid	1.1E-02	1.4E-02	1.3E-02	1.3E-02	1.3E-02	1.3E-02
N-Acetylputrescine	2.5E-03	2.9E-03	2.2E-03	3.3E-03	3.0E-03	1.8E-03
Hydroxyproline	1.7E-01	1.9E-01	1.9E-01	1.8E-01	1.9E-01	1.8E-01
cis-4-Hydroxyproline	2.6E-02	3.9E-02	3.7E-02	3.0E-02	3.8E-02	4.0E-02
Creatine	3.4E-01	4.2E-01	4.7E-01	3.4E-01	3.8E-01	3.9E-01
Ile	5.6E+00	5.3E+00	4.8E+00	5.7E+00	5.4E+00	5.3E+00
Leu	6.2E+00	6.0E+00	5.6E+00	6.5E+00	6.3E+00	6.2E+00
Asn	4.9E-01	5.2E-01	5.0E-01	4.4E-01	4.6E-01	4.7E-01
Gly-Gly	2.3E-02	2.6E-02	2.4E-02	2.7E-02	2.6E-02	2.7E-02
Ornithine	1.9E-01	2.0E-01	2.3E-01	2.1E-01	2.4E-01	2.3E-01
Thiaproline	1.9E-02	1.5E-02	9.1E-03	2.4E-02	1.2E-02	1.6E-02
Asp	7.3E+00	7.7E+00	7.2E+00	7.5E+00	7.6E+00	7.5E+00
Adenine	5.2E-03	4.4E-03	5.5E-03	5.1E-03	4.8E-03	4.4E-03
1-Methylnicotinamide	2.9E-02	3.2E-02	3.0E-02	3.4E-02	3.4E-02	3.2E-02
Urocanic acid	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
1-Methyl-4-imidazoleacetic acid	2.0E-03	2.6E-03	2.0E-03	2.1E-03	N.D.	2.1E-03
1H-Imidazole-4-propionic acid	2.4E-03	2.7E-03	2.7E-03	N.D.	2.6E-03	2.0E-03
γ-Butyrobetaine	3.9E-02	4.4E-02	4.2E-02	4.2E-02	4.4E-02	4.2E-02
Spermidine	2.9E-02	3.9E-02	3.7E-02	2.8E-02	3.7E-02	3.8E-02
Lys	1.2E+00	1.2E+00	1.1E+00	1.4E+00	1.2E+00	1.1E+00
N-Acetylserine	6.1E-02	5.7E-02	5.4E-02	5.4E-02	5.1E-02	5.6E-02
Gln	6.8E-01	6.4E-01	5.9E-01	6.7E-01	6.2E-01	6.2E-01
Glu	1.3E+00	1.4E+00	1.3E+00	1.3E+00	1.4E+00	1.4E+00
Met	8.9E-01	9.8E-01	1.0E+00	9.7E-01	1.1E+00	9.1E-01
Octopamine / Dopamine	4.0E-03	3.7E-03	3.4E-03	4.5E-03	3.4E-03	3.2E-03
His	1.4E+00	1.4E+00	1.3E+00	1.4E+00	1.4E+00	1.3E+00
O-Acetylhomoserine / 2-Aminoadipic acid	5.7E-02	6.3E-02	5.1E-02	6.3E-02	6.7E-02	6.6E-02
Carnitine	1.1E-01	1.2E-01	1.3E-01	1.2E-01	1.4E-01	1.3E-01
5-Hydroxylysine	N.D.	9.5E-04	1.2E-03	N.D.	N.D.	8.9E-04
Methionine sulfoxide	4.6E-02	3.9E-02	2.3E-02	4.0E-02	2.2E-02	3.6E-02
Phe	4.5E+00	4.4E+00	4.1E+00	4.3E+00	4.3E+00	4.1E+00
Pyridoxal	6.0E-03	6.6E-03	9.5E-03	5.2E-03	8.5E-03	7.5E-03
Pyridoxine	5.6E-02	4.9E-02	3.8E-02	6.1E-02	4.1E-02	4.2E-02
1-Methylhistidine / 3-Methylhistidine	2.9E-02	3.2E-02	3.1E-02	3.1E-02	3.1E-02	3.2E-02
N5-Ethylglutamine	1.4E-02	1.3E-02	1.4E-02	1.4E-02	1.5E-02	1.5E-02
Arg	5.9E-01	5.5E-01	4.8E-01	5.9E-01	5.5E-01	5.0E-01
Diethylaminomalonic acid	9.4E-03	9.8E-03	7.6E-03	9.3E-03	1.0E-02	9.6E-03
Citrulline	6.5E-02	6.7E-02	6.1E-02	6.4E-02	6.5E-02	6.8E-02
Cys-Gly	1.7E-03	2.1E-03	N.D.	1.8E-03	N.D.	4.3E-03
Gluconolactone	1.8E-02	1.5E-02	1.4E-02	1.5E-02	1.3E-02	1.4E-02
Galactosamine / Glucosamine	N.D.	9.7E-04	N.D.	1.1E-03	N.D.	N.D.
N-Methylnorsalsolinol	3.0E-03	2.6E-03	3.0E-03	2.4E-03	3.1E-03	4.1E-03
Tyr	2.9E+00	3.0E+00	2.7E+00	2.9E+00	2.9E+00	2.8E+00
Phosphorylcholine	6.0E+00	5.8E+00	5.3E+00	4.8E+00	4.6E+00	4.6E+00
N8-Acetylspermidine	1.1E-02	1.2E-02	9.4E-03	7.5E-03	8.1E-03	8.4E-03
Gly-Leu	N.D.	N.D.	3.3E-03	N.D.	3.4E-03	N.D.
N-Acetyllysine	3.8E-03	5.1E-03	4.1E-03	3.8E-03	5.7E-03	5.2E-03
N6-Acetyllysine	N.D.	2.9E-03	3.9E-03	2.9E-03	N.D.	3.9E-03
Homocitrulline	N.D.	N.D.	3.1E-03	2.9E-03	3.5E-03	N.D.
Gly-Asp	6.5E-02	6.7E-02	6.6E-02	6.3E-02	6.7E-02	7.0E-02
Tyrosine methyl ester	1.3E-03	1.4E-03	1.4E-03	N.D.	1.3E-03	1.5E-03
DOPA	N.D.	N.D.	N.D.	N.D.	N.D.	3.3E-03
SDMA	1.7E-03	2.2E-03	2.1E-03	3.4E-03	2.6E-03	2.4E-03
ADMA	6.0E-03	7.1E-03	6.6E-03	7.0E-03	8.3E-03	8.4E-03
Spermine	2.0E-02	2.5E-02	3.0E-02	1.7E-02	2.7E-02	2.5E-02
O-Acetylcarnitine	1.2E-01	1.2E-01	1.1E-01	1.1E-01	1.1E-01	1.1E-01
Trp	3.4E-01	3.2E-01	2.8E-01	3.2E-01	3.0E-01	3.2E-01
Carboxymethyllysine	1.8E-03	2.3E-03	2.4E-03	2.1E-03	2.5E-03	2.4E-03
Kynurenine	6.8E-03	7.1E-03	8.2E-03	6.0E-03	6.5E-03	6.7E-03
β-Ala-Lys	N.D.	N.D.	1.4E-03	N.D.	N.D.	N.D.
N-Acetylgalactosamine/N-Acetylmannosamine/N-Acetylglucosamine	9.2E-03	9.9E-03	9.1E-03	8.8E-03	7.8E-03	8.2E-03
Cystathionine	1.1E+00	1.3E+00	1.3E+00	1.2E+00	1.3E+00	1.2E+00
Carnosine	2.8E-03	2.2E-03	2.0E-03	2.7E-03	2.5E-03	1.8E-03
2'-Deoxycytidine	7.3E-04	6.3E-04	5.7E-04	9.2E-04	8.3E-04	8.0E-04
Butyrylcarnitine	8.1E-02	7.8E-02	7.5E-02	7.7E-02	8.4E-02	7.8E-02
Thr-Asp	3.5E-03	4.1E-03	3.8E-03	4.9E-03	4.8E-03	4.3E-03
Ser-Glu	8.4E-03	7.1E-03	7.4E-03	8.3E-03	8.0E-03	7.6E-03
7,8-Dihydrobiopterin	7.1E-04	1.1E-03	1.0E-03	6.2E-04	9.2E-04	8.4E-04
Ribavirin	2.1E-03	N.D.	N.D.	N.D.	N.D.	N.D.
Isovalerylcarnitine	1.1E-02	1.1E-02	1.0E-02	1.1E-02	1.2E-02	1.1E-02
γ-Glu-Cys	1.1E-02	1.3E-02	1.2E-02	9.9E-03	1.2E-02	1.1E-02
Glycerophosphocholine	1.0E-01	1.1E-01	9.6E-02	8.8E-02	9.2E-02	8.2E-02
Thiamine	9.8E-02	1.0E-01	9.6E-02	9.7E-02	9.0E-02	8.9E-02
Adenosine	3.4E-02	3.3E-02	3.8E-02	2.5E-02	2.8E-02	3.4E-02
Inosine	N.D.	1.5E-03	1.8E-03	N.D.	1.2E-03	2.0E-03
Saccharopine	9.2E-03	1.0E-02	8.7E-03	1.4E-02	1.5E-02	1.4E-02
1-Methyladenosine	8.7E-04	8.7E-04	7.1E-04	1.1E-03	6.5E-04	9.1E-04
Guanosine	1.6E-03	2.2E-03	2.3E-03	1.1E-03	1.6E-03	2.2E-03
His-Glu	9.7E-04	1.2E-03	1.2E-03	1.2E-03	1.6E-03	9.3E-04
Ophthalmic acid	4.2E-03	4.9E-03	4.0E-03	4.9E-03	4.4E-03	5.0E-03
Argininosuccinic acid	4.8E-02	5.4E-02	5.0E-02	5.0E-02	5.2E-02	5.4E-02
5'-Deoxy-5'-methylthioadenosine	7.9E-03	8.4E-03	8.7E-03	1.1E-02	8.4E-03	5.9E-03
Arg-Glu	2.1E-03	2.0E-03	1.7E-03	2.1E-03	1.6E-03	1.7E-03
Glutathione (GSSG)_divalent	2.9E+00	2.0E+00	1.9E+00	2.5E+00	1.9E+00	1.7E+00
Glutathione (GSH)	8.2E+00	1.0E+01	9.7E+00	8.6E+00	9.6E+00	9.8E+00
S-Methylglutathione	1.0E-02	1.1E-02	1.4E-02	1.0E-02	6.0E-03	5.1E-03
S-Nitrosoglutathione	2.1E-03	9.6E-04	1.2E-03	2.4E-03	2.2E-03	9.4E-04
Thiamine phosphate	5.3E-04	6.1E-04	6.8E-04	4.4E-04	5.4E-04	8.4E-04
Gibberellic acid	5.3E-03	5.9E-03	4.6E-03	5.5E-03	5.5E-03	5.5E-03
S-Lactoylglutathione	2.9E-03	4.1E-03	5.7E-03	3.0E-03	3.4E-03	2.0E-03
S-Adenosylhomocysteine	4.2E-03	5.3E-03	6.2E-03	5.0E-03	2.4E-03	2.2E-03
S-Adenosylmethionine	9.7E-02	1.0E-01	9.5E-02	9.5E-02	1.1E-01	1.0E-01
Cysteine glutathione disulfide	6.2E-03	4.3E-03	1.7E-03	5.0E-03	1.9E-03	3.8E-03
Folic acid	3.8E-03	3.7E-03	3.5E-03	2.1E-03	1.9E-03	2.1E-03
MS_METABOLITE_DATA_END
#METABOLITES
METABOLITES_START
metabolite_name
Urea
Ethanolamine
Aminoacetone
Gly
Trimethylamine N-oxide
Putrescine
β-Ala
Ala
Glycerol
1-Aminocyclopropane-1-carboxylic acid / Homoserinelactone
3-Aminoisobutyric acid
2-Aminoisobutyric acid / 2-Aminobutyric acid
GABA
Choline
Ser
Diethanolamine
Hypotaurine
1-Pyrroline 5-carboxylic acid
Creatinine
Pro
5-Aminovaleric acid
Betaine
Val
Thr
Homoserine
Cys
Nicotinamide
Taurine
Imidazole-4-acetic acid
1-Aminocyclopentanecarboxylic acid
Pipecolic acid
trans-Glutaconic acid
N-Acetylputrescine
Hydroxyproline
cis-4-Hydroxyproline
Creatine
Ile
Leu
Asn
Gly-Gly
Ornithine
Thiaproline
Asp
Adenine
1-Methylnicotinamide
Urocanic acid
1-Methyl-4-imidazoleacetic acid
1H-Imidazole-4-propionic acid
γ-Butyrobetaine
Spermidine
Lys
N-Acetylserine
Gln
Glu
Met
Octopamine / Dopamine
His
O-Acetylhomoserine / 2-Aminoadipic acid
Carnitine
5-Hydroxylysine
Methionine sulfoxide
Phe
Pyridoxal
Pyridoxine
1-Methylhistidine / 3-Methylhistidine
N5-Ethylglutamine
Arg
Diethylaminomalonic acid
Citrulline
Cys-Gly
Gluconolactone
Galactosamine / Glucosamine
N-Methylnorsalsolinol
Tyr
Phosphorylcholine
N8-Acetylspermidine
Gly-Leu
N-Acetyllysine
N6-Acetyllysine
Homocitrulline
Gly-Asp
Tyrosine methyl ester
DOPA
SDMA
ADMA
Spermine
O-Acetylcarnitine
Trp
Carboxymethyllysine
Kynurenine
β-Ala-Lys
N-Acetylgalactosamine/N-Acetylmannosamine/N-Acetylglucosamine
Cystathionine
Carnosine
2'-Deoxycytidine
Butyrylcarnitine
Thr-Asp
Ser-Glu
7,8-Dihydrobiopterin
Ribavirin
Isovalerylcarnitine
γ-Glu-Cys
Glycerophosphocholine
Thiamine
Adenosine
Inosine
Saccharopine
1-Methyladenosine
Guanosine
His-Glu
Ophthalmic acid
Argininosuccinic acid
5'-Deoxy-5'-methylthioadenosine
Arg-Glu
Glutathione (GSSG)_divalent
Glutathione (GSH)
S-Methylglutathione
S-Nitrosoglutathione
Thiamine phosphate
Gibberellic acid
S-Lactoylglutathione
S-Adenosylhomocysteine
S-Adenosylmethionine
Cysteine glutathione disulfide
Folic acid
METABOLITES_END
#END