#METABOLOMICS WORKBENCH efahy_20230425_000007 DATATRACK_ID:3899 STUDY_ID:ST002589 ANALYSIS_ID:AN004261 PROJECT_ID:PR001020
VERSION             	1
CREATED_ON             	April 25, 2023, 7:56 am
#PROJECT
PR:PROJECT_TITLE                 	Molecular Transducer of Physical Activity Consortium
PR:PROJECT_SUMMARY               	MoTrPAC is a national research consortium designed to discover and perform
PR:PROJECT_SUMMARY               	preliminary characterization of the range of molecular transducers (the
PR:PROJECT_SUMMARY               	"molecular map") that underlie the effects of physical activity in humans. The
PR:PROJECT_SUMMARY               	program's goal is to study the molecular changes that occur during and after
PR:PROJECT_SUMMARY               	exercise and ultimately to advance the understanding of how physical activity
PR:PROJECT_SUMMARY               	improves and preserves health. Preclinical and clinical studies will examine the
PR:PROJECT_SUMMARY               	systemic effects of endurance and resistance exercise across a range of ages and
PR:PROJECT_SUMMARY               	fitness levels by molecular probing of multiple tissues before and after acute
PR:PROJECT_SUMMARY               	and chronic exercise. This program is the largest targeted NIH investment of
PR:PROJECT_SUMMARY               	funds into the mechanisms of how physical activity improves health and prevents
PR:PROJECT_SUMMARY               	disease. The MoTrPAC program is supported by the NIH Common Fund and is managed
PR:PROJECT_SUMMARY               	by a trans-agency working group representing multiple NIH institutes and
PR:PROJECT_SUMMARY               	centers, led by the NIH Office of Strategic Coordination, National Institute of
PR:PROJECT_SUMMARY               	Arthritis and Musculoskeletal and Skin Diseases, National Institute of Diabetes
PR:PROJECT_SUMMARY               	and Digestive and Kidney Diseases, National Institute on Aging, and National
PR:PROJECT_SUMMARY               	Institute of Biomedical Imaging and Bioengineering. MoTrPAC Steering Committee:
PR:PROJECT_SUMMARY               	Wendy Kohrt, Chair, Russ Tracy, Co-Chair; NIH Program Manager, Concepcion
PR:PROJECT_SUMMARY               	Nierras. Euan Ashley and Matthew Wheeler are the PIs for the Motrpac
PR:PROJECT_SUMMARY               	Bioinformatics / Data Coordination Center.
PR:INSTITUTE                     	MoTrPAC
PR:LAST_NAME                     	Ashley
PR:FIRST_NAME                    	Euan A.
PR:ADDRESS                       	300 Pasteur Dr Rm A265. MC 5319. Stanford CA 94305. USA
PR:EMAIL                         	euan@stanford.edu
PR:PHONE                         	(650) 498-4900
#STUDY
ST:STUDY_TITLE                   	MoTrPAC: Endurance exercise training study in young adult rats, Rat Hippocampus
ST:STUDY_TITLE                   	Powder - Targeted Ethanolamides
ST:STUDY_SUMMARY                 	The goal of the endurance exercise training study in young adult rats (internal
ST:STUDY_SUMMARY                 	code: PASS1B-06) was to perform exercise training studies in adult (6 month)
ST:STUDY_SUMMARY                 	F344 rats, and from these rats collect as many tissues as feasible in order to
ST:STUDY_SUMMARY                 	provide high quality samples for detailed analysis by chemical analysis sites.
ST:STUDY_SUMMARY                 	Tissues were collected from 10-12 rats sedentary control rats concurrent with
ST:STUDY_SUMMARY                 	the collection of the 8-week training groups. The 8-week training group and
ST:STUDY_SUMMARY                 	controls were from the same cohort and same age at euthanasia (either 8). For
ST:STUDY_SUMMARY                 	the older age group, an additional set of controls (n=5-6) were collected with
ST:STUDY_SUMMARY                 	the 1-2 week training group. Rats were either sedentary or underwent an exercise
ST:STUDY_SUMMARY                 	training program. Rats were exercised on the rodent treadmill 5 days per week
ST:STUDY_SUMMARY                 	using a progressive training protocol designed to exercise the rats at
ST:STUDY_SUMMARY                 	approximately 70% of VO2max as outlined in the Table on the next page. Training
ST:STUDY_SUMMARY                 	was performed no earlier than 10:00 am and no later than 5:00 pm over 5
ST:STUDY_SUMMARY                 	consecutive days per week. Training was initiated with the treadmill set at 70%
ST:STUDY_SUMMARY                 	of VO2 max (see tables) and 5 degrees grade for 20 minutes. The duration of
ST:STUDY_SUMMARY                 	exercise was increased by one minute each day until day 31 of training (start of
ST:STUDY_SUMMARY                 	week 7), when a duration of 50 min was reached. Speed and grade of each training
ST:STUDY_SUMMARY                 	session increased in larger increments due to treadmill parameters. The highest
ST:STUDY_SUMMARY                 	intensity and duration of training began on day 31. This intensity was
ST:STUDY_SUMMARY                 	maintained for the final 10 days of the protocol to ensure steady state had been
ST:STUDY_SUMMARY                 	achieved. If any rats were unable to perform at least 4 days of training per
ST:STUDY_SUMMARY                 	week they were removed from the study and euthanized. It is important to note
ST:STUDY_SUMMARY                 	that the starting treadmill speed varied depending on the sex and age of the
ST:STUDY_SUMMARY                 	rat. The initial and maximum speeds were based on VO2max measurements obtained
ST:STUDY_SUMMARY                 	during the pre-training testing of the compliant rats. Rats assigned to the
ST:STUDY_SUMMARY                 	control group followed a schedule similar to the training group. They were
ST:STUDY_SUMMARY                 	placed in one lane on the treadmill for 15 minutes/day, 5 days per week. The
ST:STUDY_SUMMARY                 	treadmill was set at 0 m/min at an incline that corresponded to the incline
ST:STUDY_SUMMARY                 	being used by the training group.
ST:INSTITUTE                     	Emory University
ST:DEPARTMENT                    	Biochemistry
ST:LABORATORY                    	Integrated Lipidomics and Metabolomics Core
ST:LAST_NAME                     	Ortlund
ST:FIRST_NAME                    	Eric
ST:ADDRESS                       	1510 Clifton Rd. NE, Room G235, Atlanta, GA 30322
ST:EMAIL                         	eortlun@emory.edu
ST:PHONE                         	(404) 727-5014
#SUBJECT
SU:SUBJECT_TYPE                  	Mammal
SU:SUBJECT_SPECIES               	Rattus norvegicus
SU:TAXONOMY_ID                   	10116
#SUBJECT_SAMPLE_FACTORS:          	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data
SUBJECT_SAMPLE_FACTORS           	-	QC_prerun_1	Group:QC-PreRun | Timepoint:0 hour | Sex:-	raw_file=MoTrPAC_hippocampus_pass1b_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	-	QC_prerun_2	Group:QC-PreRun | Timepoint:0 hour | Sex:-	raw_file=MoTrPAC_hippocampus_pass1b_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	-	QC_prerun_3	Group:QC-PreRun | Timepoint:0 hour | Sex:-	raw_file=MoTrPAC_hippocampus_pass1b_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	-	QC_blank_4	Group:QC-Blank | Timepoint:0 hour | Sex:-	raw_file=MoTrPAC_hippocampus_pass1b_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	-	QC_blank_5	Group:QC-Blank | Timepoint:0 hour | Sex:-	raw_file=MoTrPAC_hippocampus_pass1b_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	-	QC_blank_6	Group:QC-Blank | Timepoint:0 hour | Sex:-	raw_file=MoTrPAC_hippocampus_pass1b_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	-	QC_blank_7	Group:QC-Blank | Timepoint:0 hour | Sex:-	raw_file=MoTrPAC_hippocampus_pass1b_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	22D700B9-EDD0-4BC8-8155-EB9C39417F81	90280015208	Group:Training | Timepoint:4 weeks of training | Sex:Male	raw_file=MoTrPAC_hippocampus_pass1b_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	3D5BC47C-BB3D-45A2-8DB8-D7ADCD51997C	90412015208	Group:Training | Timepoint:4 weeks of training | Sex:Female	raw_file=MoTrPAC_hippocampus_pass1b_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	AEB1611A-F29E-43F8-B9F3-A2BC4F9EB504	90444015208	Group:Training | Timepoint:2 weeks of training | Sex:Male	raw_file=MoTrPAC_hippocampus_pass1b_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	0D5071F5-D166-4CF1-BE8B-EB4DF6E04C0E	90251015208	Group:Training | Timepoint:8 weeks of training or control time | Sex:Female	raw_file=MoTrPAC_hippocampus_pass1b_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	F9BEE1FA-E2DB-4BE4-A79C-99D585D6FD17	90265015208	Group:Control | Timepoint:8 weeks of training or control time | Sex:Female	raw_file=MoTrPAC_hippocampus_pass1b_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	8904857C-2073-4BBA-AC7C-1EC239E5BFF7	90581015208	Group:Training | Timepoint:2 weeks of training | Sex:Female	raw_file=MoTrPAC_hippocampus_pass1b_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	04C84D7E-FC8C-422F-AE4B-CAFADB63407B	90259015208	Group:Training | Timepoint:8 weeks of training or control time | Sex:Female	raw_file=MoTrPAC_hippocampus_pass1b_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	07AF7369-F082-4CA7-99D5-D973F4A162C9	90252015208	Group:Control | Timepoint:8 weeks of training or control time | Sex:Female	raw_file=MoTrPAC_hippocampus_pass1b_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	BC49E4EF-E2FE-4269-BAF0-461F768BD95D	90217015208	Group:Control | Timepoint:8 weeks of training or control time | Sex:Male	raw_file=MoTrPAC_hippocampus_pass1b_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	DC8B35E8-4AA8-4829-8569-F223D069620C	90578015208	Group:Training | Timepoint:2 weeks of training | Sex:Female	raw_file=MoTrPAC_hippocampus_pass1b_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	-	QC_18	Group:QC-Pooled | Timepoint:0 hour | Sex:-	raw_file=MoTrPAC_hippocampus_pass1b_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	D350D633-8D95-4783-99F2-BB8294D2EC32	90423015208	Group:Training | Timepoint:1 week of training or control time | Sex:Male	raw_file=MoTrPAC_hippocampus_pass1b_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	3719CB62-B551-49BE-94A0-E51DFE13D30D	90225015208	Group:Training | Timepoint:8 weeks of training or control time | Sex:Male	raw_file=MoTrPAC_hippocampus_pass1b_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	0559B193-BED3-4170-B045-10A2B6441CB6	90229015208	Group:Control | Timepoint:8 weeks of training or control time | Sex:Male	raw_file=MoTrPAC_hippocampus_pass1b_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	6527EC12-E7EB-4F17-BF0A-0AC7CD1819A0	90441015208	Group:Training | Timepoint:2 weeks of training | Sex:Male	raw_file=MoTrPAC_hippocampus_pass1b_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	0645F621-878C-4C9B-84A6-58862BAA17D9	90564015208	Group:Training | Timepoint:1 week of training or control time | Sex:Female	raw_file=MoTrPAC_hippocampus_pass1b_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	2B969165-1A52-43AA-8FD9-39AFA62F094F	90267015208	Group:Training | Timepoint:8 weeks of training or control time | Sex:Female	raw_file=MoTrPAC_hippocampus_pass1b_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	6D680EA2-C62B-4A35-8B6E-2ED6A79CB017	90421015208	Group:Training | Timepoint:1 week of training or control time | Sex:Male	raw_file=MoTrPAC_hippocampus_pass1b_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	955498F4-70A0-4D50-A2BA-619A8FED8FEF	90222015208	Group:Training | Timepoint:8 weeks of training or control time | Sex:Male	raw_file=MoTrPAC_hippocampus_pass1b_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	0DBFEB04-1B58-43CF-BAFD-9AA9915B67F6	90426015208	Group:Training | Timepoint:1 week of training or control time | Sex:Male	raw_file=MoTrPAC_hippocampus_pass1b_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	0BD282C7-7832-4581-9089-08A32D557034	90218015208	Group:Training | Timepoint:8 weeks of training or control time | Sex:Male	raw_file=MoTrPAC_hippocampus_pass1b_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	-	QC_29	Group:QC-Pooled | Timepoint:0 hour | Sex:-	raw_file=MoTrPAC_hippocampus_pass1b_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	68083256-8DAA-4185-B446-0CAF600474B5	90567015208	Group:Training | Timepoint:1 week of training or control time | Sex:Female	raw_file=MoTrPAC_hippocampus_pass1b_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	EA5C8932-9325-4DA5-96BB-50CD49CEDD10	90560015208	Group:Training | Timepoint:1 week of training or control time | Sex:Female	raw_file=MoTrPAC_hippocampus_pass1b_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	569A9229-04E8-414A-A195-FE9E8F1B66AB	90289015208	Group:Training | Timepoint:4 weeks of training | Sex:Male	raw_file=MoTrPAC_hippocampus_pass1b_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	FDA87A8D-3634-4C42-9A1A-0EA03B58BB01	90449015208	Group:Training | Timepoint:2 weeks of training | Sex:Male	raw_file=MoTrPAC_hippocampus_pass1b_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	FCE9ABE6-B60B-4934-BAE5-56AF8B11ECEC	90239015208	Group:Control | Timepoint:8 weeks of training or control time | Sex:Male	raw_file=MoTrPAC_hippocampus_pass1b_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	69FD4884-786D-4F2A-97B1-6174FBD25D60	90248015208	Group:Control | Timepoint:8 weeks of training or control time | Sex:Female	raw_file=MoTrPAC_hippocampus_pass1b_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	CDCFA3F1-07ED-4737-A36D-15EFEC325AA5	90450015208	Group:Training | Timepoint:2 weeks of training | Sex:Male	raw_file=MoTrPAC_hippocampus_pass1b_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	10E282EB-1DBA-406E-BD5D-81027083A01D	90430015208	Group:Training | Timepoint:1 week of training or control time | Sex:Male	raw_file=MoTrPAC_hippocampus_pass1b_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	B137E81D-A9DE-4E3A-8E2B-AD6C22920B7E	90576015208	Group:Training | Timepoint:2 weeks of training | Sex:Female	raw_file=MoTrPAC_hippocampus_pass1b_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	90CFE5A4-76FE-4458-A2B1-A66F0B60118E	90232015208	Group:Control | Timepoint:8 weeks of training or control time | Sex:Male	raw_file=MoTrPAC_hippocampus_pass1b_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	-	QC_40	Group:QC-Pooled | Timepoint:0 hour | Sex:-	raw_file=MoTrPAC_hippocampus_pass1b_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	FE012DC0-AF32-4C70-A4D4-F042B8BC4EC6	90420015208	Group:Training | Timepoint:4 weeks of training | Sex:Female	raw_file=MoTrPAC_hippocampus_pass1b_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	B73AB139-7210-4498-9CCF-E1DBE6EBA71C	90559015208	Group:Training | Timepoint:1 week of training or control time | Sex:Female	raw_file=MoTrPAC_hippocampus_pass1b_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	89D6F615-D17C-4EB9-88EB-A602940FF179	90416015208	Group:Training | Timepoint:4 weeks of training | Sex:Female	raw_file=MoTrPAC_hippocampus_pass1b_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	0409BED3-F9FE-4C06-9920-74F5E5CA04A3	90587015208	Group:Training | Timepoint:2 weeks of training | Sex:Female	raw_file=MoTrPAC_hippocampus_pass1b_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	53F914B0-FD4F-4340-84A6-48341EAB3798	90422015208	Group:Training | Timepoint:1 week of training or control time | Sex:Male	raw_file=MoTrPAC_hippocampus_pass1b_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	6A013AA1-4D1E-4B75-B691-6BA22D928E51	90571015208	Group:Training | Timepoint:1 week of training or control time | Sex:Female	raw_file=MoTrPAC_hippocampus_pass1b_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	4E974304-21EB-42E8-A729-A7FB6B406E64	90439015208	Group:Training | Timepoint:2 weeks of training | Sex:Male	raw_file=MoTrPAC_hippocampus_pass1b_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	CFE06932-9314-4BCC-B2D9-73883D5FCDFB	90223015208	Group:Training | Timepoint:8 weeks of training or control time | Sex:Male	raw_file=MoTrPAC_hippocampus_pass1b_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	0A4A7935-3AF4-463F-84E3-DCF07671509D	90258015208	Group:Training | Timepoint:8 weeks of training or control time | Sex:Female	raw_file=MoTrPAC_hippocampus_pass1b_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	9F3E1040-D409-421E-B635-2572DC20ED89	90283015208	Group:Training | Timepoint:4 weeks of training | Sex:Male	raw_file=MoTrPAC_hippocampus_pass1b_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	-	QC_blank_51	Group:QC-Blank | Timepoint:0 hour | Sex:-	raw_file=MoTrPAC_hippocampus_pass1b_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	F65955CE-E49F-48C7-B861-02A2FDF6F9B8	90227015208	Group:Training | Timepoint:8 weeks of training or control time | Sex:Male	raw_file=MoTrPAC_hippocampus_pass1b_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	2479BEE5-2A38-4D09-B15B-B1715F561A9F	90254015208	Group:Training | Timepoint:8 weeks of training or control time | Sex:Female	raw_file=MoTrPAC_hippocampus_pass1b_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	A9CE3E3D-31A0-4443-8A7F-8FD831D79248	90410015208	Group:Training | Timepoint:4 weeks of training | Sex:Female	raw_file=MoTrPAC_hippocampus_pass1b_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	1CA4210C-3CE6-4A5A-996B-673EBDEB3B81	90281015208	Group:Training | Timepoint:4 weeks of training | Sex:Male	raw_file=MoTrPAC_hippocampus_pass1b_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	F0D83B29-8EFA-4CDE-B1AA-2358AC220ECF	90237015208	Group:Control | Timepoint:8 weeks of training or control time | Sex:Male	raw_file=MoTrPAC_hippocampus_pass1b_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	1F9BD644-303F-43E7-94BE-FF6654306F5E	90585015208	Group:Training | Timepoint:2 weeks of training | Sex:Female	raw_file=MoTrPAC_hippocampus_pass1b_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	A77AC89A-1D9E-4516-9DA4-2BE99C8126EF	90266015208	Group:Control | Timepoint:8 weeks of training or control time | Sex:Female	raw_file=MoTrPAC_hippocampus_pass1b_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	E4271AF1-899A-4A65-9D8D-BF18CEE3F1CF	90292015208	Group:Training | Timepoint:4 weeks of training | Sex:Male	raw_file=MoTrPAC_hippocampus_pass1b_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	1628FB23-6F66-47D4-8D82-63C87726F0FD	90245015208	Group:Control | Timepoint:8 weeks of training or control time | Sex:Female	raw_file=MoTrPAC_hippocampus_pass1b_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	-	QC_blank_61	Group:QC-Blank | Timepoint:0 hour | Sex:-	raw_file=MoTrPAC_hippocampus_pass1b_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	-	QC_blank_62	Group:QC-Blank | Timepoint:0 hour | Sex:-	raw_file=MoTrPAC_hippocampus_pass1b_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	-	QC_blank_63	Group:QC-Blank | Timepoint:0 hour | Sex:-	raw_file=MoTrPAC_hippocampus_pass1b_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	-	QC_blank_64	Group:QC-Blank | Timepoint:0 hour | Sex:-	raw_file=MoTrPAC_hippocampus_pass1b_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	-	QC_Standard_65	Group:QC-ExternalStandard | Timepoint:0 hour | Sex:-	raw_file=MoTrPAC_hippocampus_pass1b_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	-	QC_Standard_66	Group:QC-ExternalStandard | Timepoint:0 hour | Sex:-	raw_file=MoTrPAC_hippocampus_pass1b_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	-	QC_Standard_67	Group:QC-ExternalStandard | Timepoint:0 hour | Sex:-	raw_file=MoTrPAC_hippocampus_pass1b_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	-	QC_Standard_68	Group:QC-ExternalStandard | Timepoint:0 hour | Sex:-	raw_file=MoTrPAC_hippocampus_pass1b_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	-	QC_Standard_69	Group:QC-ExternalStandard | Timepoint:0 hour | Sex:-	raw_file=MoTrPAC_hippocampus_pass1b_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	-	QC_Standard_70	Group:QC-ExternalStandard | Timepoint:0 hour | Sex:-	raw_file=MoTrPAC_hippocampus_pass1b_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	-	QC_Standard_71	Group:QC-ExternalStandard | Timepoint:0 hour | Sex:-	raw_file=MoTrPAC_hippocampus_pass1b_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	-	QC_blank_72	Group:QC-Blank | Timepoint:0 hour | Sex:-	raw_file=MoTrPAC_hippocampus_pass1b_pos_V1-20200828.wiff
SUBJECT_SAMPLE_FACTORS           	-	QC_blank_73	Group:QC-Blank | Timepoint:0 hour | Sex:-	raw_file=MoTrPAC_hippocampus_pass1b_pos_V1-20200828.wiff
#COLLECTION
CO:SAMPLE_TYPE                   	Hippocampus
#TREATMENT
TR:TREATMENT_SUMMARY             	-
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	Purification of oxylipids with SPE column
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_SUMMARY        	Lipids were separated by liquid chromatography with 20 minutes gradient
CH:CHROMATOGRAPHY_TYPE           	Reversed phase
CH:INSTRUMENT_NAME               	ExionLC AC
CH:COLUMN_NAME                   	ThermoScientific Accucore C18 (100 x 4.6mm, 2.6um)
CH:SOLVENT_A                     	water with 10 mM ammonium acetate
CH:SOLVENT_B                     	acetonitrile with 10 mM ammonium acetate
CH:FLOW_GRADIENT                 	t=0 min: 90%/10% (%A/%B) @ 0.5 ml/min | t=0.5 min: 90%/10% (%A/%B) @ 0.5 ml/min
CH:FLOW_GRADIENT                 	| t=1 min: 50%/50% (%A/%B) @ 0.5 ml/min | t=2 min: 50%/50% (%A/%B) @ 0.5 ml/min
CH:FLOW_GRADIENT                 	| t=2.1 min: 25%/75% (%A/%B) @ 0.5 ml/min | t=5 min: 25%/75% (%A/%B) @ 0.5
CH:FLOW_GRADIENT                 	ml/min | t=7 min: 15%/85% (%A/%B) @ 0.5 ml/min | t=12 min: 15%/85% (%A/%B) @ 0.5
CH:FLOW_GRADIENT                 	ml/min | t=12.1 min: 90%/10% (%A/%B) @ 0.5 ml/min | t=18 min: 90%/10% (%A/%B) @
CH:FLOW_GRADIENT                 	0.5 ml/min
CH:FLOW_RATE                     	0.5 ml/min
CH:COLUMN_TEMPERATURE            	50C
#ANALYSIS
AN:ANALYSIS_TYPE                 	MS
AN:ANALYSIS_DETAILS:             	Peak integration, concentrations calculated base on standards curves equations,
AN:ANALYSIS_DETAILS:             	in the result file data represented in ng/mg of tissue
#MS
MS:INSTRUMENT_TYPE               	Triple quadrupole
MS:INSTRUMENT_NAME               	ABI Sciex 5500 Qtrap
MS:MS_TYPE                       	ESI
MS:ION_MODE                      	POSITIVE
MS:MS_COMMENTS                   	Multiple reaction monitoring mode (MRM)
#MS_METABOLITE_DATA
MS_METABOLITE_DATA:UNITS	ng/uL
MS_METABOLITE_DATA_START
Samples	QC_prerun_1	QC_prerun_2	QC_prerun_3	QC_blank_4	QC_blank_5	QC_blank_6	QC_blank_7	90280015208	90412015208	90444015208	90251015208	90265015208	90581015208	90259015208	90252015208	90217015208	90578015208	QC_18	90423015208	90225015208	90229015208	90441015208	90564015208	90267015208	90421015208	90222015208	90426015208	90218015208	QC_29	90567015208	90560015208	90289015208	90449015208	90239015208	90248015208	90450015208	90430015208	90576015208	90232015208	QC_40	90420015208	90559015208	90416015208	90587015208	90422015208	90571015208	90439015208	90223015208	90258015208	90283015208	QC_blank_51	90227015208	90254015208	90410015208	90281015208	90237015208	90585015208	90266015208	90292015208	90245015208	QC_blank_61	QC_blank_62	QC_blank_63	QC_blank_64	QC_Standard_65	QC_Standard_66	QC_Standard_67	QC_Standard_68	QC_Standard_69	QC_Standard_70	QC_Standard_71	QC_blank_72	QC_blank_73
Behenoyl-EA	0.003964098	0.001777509	0.001982117	0.001328546	0.00140703	0.001388422	0.001189621	0.00165798	0.001522529	0.001918558	0.001465876	0.001005079	0.001684589	0.001211289	0.001224305	0.00110254	0.001131339	0.000771535	0.000994431	0.000797598	0.000945083	0.001233467	0.001146472	0.000828021	0.000894815	0.000881347	0.000771666	0.000702123	0.000685624	0.000789377	0.000915307	0.000741946	0.000721268	0.000696236	0.000737412	0.000730355	0.000757761	0.000785333	0.000792944	0.00050765	0.000656013	0.000693396	0.000691249	0.00069926	0.000644885	0.000645644	0.000654437	0.000645713	0.000646149	0.000655944	0.000473721	0.000609586	0.000682871	0.000563807	0.0006719	0.000689724	0.000639844	0.000735583	0.000587168	0.000657529	0.000507216	0.00046222	0.000494372	0.004720179	0.181686047	0.363372093	0.726744186	1.453488372	2.906976744	5.813953488	11.62790698	0.016144202	0
Arachidonoyl-EA	0.000423664	0.00042586	0.00046156	0.000509983	0.000240146	0.000211425	0.000207	0.000291587	0.000261212	0.00055569	0.000235225	0.0002353	0.000426011	0.000190526	0.00020669	0.000317601	0.000169946	7.48393e-05	0.000163579	0.000192004	0.000247213	0.000174812	0.000328729	0.000130113	0.000170778	0.000171516	0.000127875	0.000215049	4.75353e-05	9.2389e-05	0.000238985	0.000187345	0.00012262	0.000161137	0.000167152	0.000228223	0.000202519	0.000140883	0.000209513	5.58783e-05	0.000223223	0.000284731	0.000251436	0.000137353	0.000209119	0.000264711	0.000149865	0.000215756	0.000105734	0.000184942	3.547e-05	0.000200129	0.000158482	0.000150123	0.000145106	0.000344065	0.00015613	9.82673e-05	0.000186576	0.000264549	2.5986e-05	0.00030453	3.1138e-05	0.000247314	0.163517442	0.327034884	0.654069767	1.308139535	2.61627907	5.23255814	10.46511628	0.000398697	0.000422536
MS_METABOLITE_DATA_END
#METABOLITES
METABOLITES_START
metabolite_name	formula	mz	neutral_mass	refmet_name	rt
Behenoyl-EA	C24H49NO2	384	383.3763	Behenoyl-EA	11.76
Arachidonoyl-EA	C22H37NO2	348.2	347.2824	Arachidonoyl-EA	5.59
METABOLITES_END
#END