#METABOLOMICS WORKBENCH LIUYUP_20230820_174408 DATATRACK_ID:4233 STUDY_ID:ST002838 ANALYSIS_ID:AN004662 PROJECT_ID:PR001777 VERSION 1 CREATED_ON August 31, 2023, 8:32 pm #PROJECT PR:PROJECT_TITLE Quantitative subcellular reconstruction reveals a lipid-mediated inter-organelle PR:PROJECT_TITLE biogenesis network PR:PROJECT_SUMMARY Mitochondria are key regulators in a variety of cellular processes and have been PR:PROJECT_SUMMARY shown to interact with multiple other organelles, which use their own unique PR:PROJECT_SUMMARY compartmentalised environments and work in concert to drive key molecular PR:PROJECT_SUMMARY processes required for cell function. Mitochondria, peroxisomes, Golgi, and ER PR:PROJECT_SUMMARY all exchange fatty acids to allow for specialised processing and subsequent use PR:PROJECT_SUMMARY by each specific organelle. While these processes suggest inter-organellar PR:PROJECT_SUMMARY dependency for cell functions, the nature of this interconnection and the PR:PROJECT_SUMMARY implications for cell metabolism have yet to be elucidated. To address this PR:PROJECT_SUMMARY knowledge gap, performed a whole-genome screen for mitochondrial stress and PR:PROJECT_SUMMARY established knockout cell lines for genes highlighted in the screen that are PR:PROJECT_SUMMARY important for the biogenesis of peroxisomes, Golgi, and the ER. The PR:PROJECT_SUMMARY comprehensive lipidomics analysis on the wild-type and five knockout CAL51 cell PR:PROJECT_SUMMARY lines illustrating that dysfunction of a single organelle induces a cell-wide PR:PROJECT_SUMMARY network of organelle dysfunctions. Finally, we show that a specific class of PR:PROJECT_SUMMARY lipids can repair the mitochondrial network in cells with different organelle PR:PROJECT_SUMMARY biogenesis defects. PR:INSTITUTE university of Melbourne PR:DEPARTMENT school of chemistry PR:LABORATORY Reid Lab PR:LAST_NAME Peng PR:FIRST_NAME Liuyu PR:ADDRESS 30 Flemington Rd, Parkville VIC 3052, Parkville, Victoria, 3052, Australia PR:EMAIL liuyup@student.unimelb.edu.au PR:PHONE 0481794685 #STUDY ST:STUDY_TITLE Quantitative subcellular reconstruction reveals a lipid-mediated inter organelle ST:STUDY_TITLE biogenesis network ST:STUDY_SUMMARY Lipidomics analysis on wild-type and five non-mitochondria organelle biogenesis ST:STUDY_SUMMARY gene knockout CAL51 cell lines ST:INSTITUTE University of Melbourne ST:DEPARTMENT school of chemistry ST:LABORATORY Reid Lab ST:LAST_NAME Peng ST:FIRST_NAME Liuyu ST:ADDRESS 30 Flemington Rd ST:EMAIL liuyup@student.unimelb.edu.au ST:PHONE 0481794685 #SUBJECT SU:SUBJECT_TYPE Human SU:SUBJECT_SPECIES Homo sapiens SU:TAXONOMY_ID 9606 #FACTORS #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data SUBJECT_SAMPLE_FACTORS - S1 GenoType:Wild-type | Treatment:Control | Sample.condition:Wholecell RAW_FILE_NAME=WT-WC-NonDerPos-1; RAW_FILE_NAME=WT-WC-NonDerNeg-1; RAW_FILE_NAME=WT-WC-DerPos-1; RAW_FILE_NAME=WT-WC-Faims-1 SUBJECT_SAMPLE_FACTORS - S2 GenoType:Wild-type | Treatment:Control | Sample.condition:Wholecell RAW_FILE_NAME=WT-WC-NonDerPos-2; RAW_FILE_NAME=WT-WC-NonDerNeg-2; RAW_FILE_NAME=WT-WC-DerPos-2; RAW_FILE_NAME=WT-WC-Faims-2 SUBJECT_SAMPLE_FACTORS - S3 GenoType:Wild-type | Treatment:Control | Sample.condition:Wholecell RAW_FILE_NAME=WT-WC-NonDerPos-3; RAW_FILE_NAME=WT-WC-NonDerNeg-3; RAW_FILE_NAME=WT-WC-DerPos-3; RAW_FILE_NAME=WT-WC-Faims-3 SUBJECT_SAMPLE_FACTORS - S4 GenoType:Wild-type | Treatment:Control | Sample.condition:Wholecell RAW_FILE_NAME=WT-WC-NonDerPos-4; RAW_FILE_NAME=WT-WC-NonDerNeg-4; RAW_FILE_NAME=WT-WC-DerPos-4; RAW_FILE_NAME=WT-WC-Faims-4 SUBJECT_SAMPLE_FACTORS - S5 GenoType:Wild-type | Treatment:Control | Sample.condition:Wholecell RAW_FILE_NAME=WT-WC-NonDerPos-5; RAW_FILE_NAME=WT-WC-NonDerNeg-5; RAW_FILE_NAME=WT-WC-DerPos-5; RAW_FILE_NAME=WT-WC-Faims-5 SUBJECT_SAMPLE_FACTORS - S6 GenoType:Wild-type | Treatment:Control | Sample.condition:Mitochondria RAW_FILE_NAME=WT-MT-NonDerPos-1; RAW_FILE_NAME=WT-MT-NonDerNeg-1; RAW_FILE_NAME=WT-MT-DerPos-1; RAW_FILE_NAME=WT-MT-Faims-1 SUBJECT_SAMPLE_FACTORS - S7 GenoType:Wild-type | Treatment:Control | Sample.condition:Mitochondria RAW_FILE_NAME=WT-MT-NonDerPos-2; RAW_FILE_NAME=WT-MT-NonDerNeg-2; RAW_FILE_NAME=WT-MT-DerPos-2; RAW_FILE_NAME=WT-MT-Faims-2 SUBJECT_SAMPLE_FACTORS - S8 GenoType:Wild-type | Treatment:Control | Sample.condition:Mitochondria RAW_FILE_NAME=WT-MT-NonDerPos-3; RAW_FILE_NAME=WT-MT-NonDerNeg-3; RAW_FILE_NAME=WT-MT-DerPos-3; RAW_FILE_NAME=WT-MT-Faims-3 SUBJECT_SAMPLE_FACTORS - S9 GenoType:Wild-type | Treatment:Control | Sample.condition:Mitochondria RAW_FILE_NAME=WT-MT-NonDerPos-4; RAW_FILE_NAME=WT-MT-NonDerNeg-4; RAW_FILE_NAME=WT-MT-DerPos-4; RAW_FILE_NAME=WT-MT-Faims-4 SUBJECT_SAMPLE_FACTORS - S10 GenoType:Wild-type | Treatment:Control | Sample.condition:Mitochondria RAW_FILE_NAME=WT-MT-NonDerPos-5; RAW_FILE_NAME=WT-MT-NonDerNeg-5; RAW_FILE_NAME=WT-MT-DerPos-5; RAW_FILE_NAME=WT-MT-Faims-5 SUBJECT_SAMPLE_FACTORS - S11 GenoType:Pex26-knockout | Treatment:Control | Sample.condition:Wholecell RAW_FILE_NAME=Pex26-WC-NonDerPos-1; RAW_FILE_NAME=Pex26-WC-NonDerNeg-1; RAW_FILE_NAME=Pex26-WC-DerPos-1; RAW_FILE_NAME=Pex26-WC-Faims-1 SUBJECT_SAMPLE_FACTORS - S12 GenoType:Pex26-knockout | Treatment:Control | Sample.condition:Wholecell RAW_FILE_NAME=Pex26-WC-NonDerPos-2; RAW_FILE_NAME=Pex26-WC-NonDerNeg-2; RAW_FILE_NAME=Pex26-WC-DerPos-2; RAW_FILE_NAME=Pex26-WC-Faims-2 SUBJECT_SAMPLE_FACTORS - S13 GenoType:Pex26-knockout | Treatment:Control | Sample.condition:Wholecell RAW_FILE_NAME=Pex26-WC-NonDerPos-3; RAW_FILE_NAME=Pex26-WC-NonDerNeg-3; RAW_FILE_NAME=Pex26-WC-DerPos-3; RAW_FILE_NAME=Pex26-WC-Faims-3 SUBJECT_SAMPLE_FACTORS - S14 GenoType:Pex26-knockout | Treatment:Control | Sample.condition:Wholecell RAW_FILE_NAME=Pex26-WC-NonDerPos-4; RAW_FILE_NAME=Pex26-WC-NonDerNeg-4; RAW_FILE_NAME=Pex26-WC-DerPos-4; RAW_FILE_NAME=Pex26-WC-Faims-4 SUBJECT_SAMPLE_FACTORS - S15 GenoType:Pex26-knockout | Treatment:Control | Sample.condition:Wholecell RAW_FILE_NAME=Pex26-WC-NonDerPos-5; RAW_FILE_NAME=Pex26-WC-NonDerNeg-5; RAW_FILE_NAME=Pex26-WC-DerPos-5; RAW_FILE_NAME=Pex26-WC-Faims-5 SUBJECT_SAMPLE_FACTORS - S16 GenoType:Pex26-knockout | Treatment:Control | Sample.condition:Mitochondria RAW_FILE_NAME=Pex26-MT-NonDerPos-1; RAW_FILE_NAME=Pex26-MT-NonDerNeg-1; RAW_FILE_NAME=Pex26-MT-DerPos-1; RAW_FILE_NAME=Pex26-MT-Faims-1 SUBJECT_SAMPLE_FACTORS - S17 GenoType:Pex26-knockout | Treatment:Control | Sample.condition:Mitochondria RAW_FILE_NAME=Pex26-MT-NonDerPos-2; RAW_FILE_NAME=Pex26-MT-NonDerNeg-2; RAW_FILE_NAME=Pex26-MT-DerPos-2; RAW_FILE_NAME=Pex26-MT-Faims-2 SUBJECT_SAMPLE_FACTORS - S18 GenoType:Pex26-knockout | Treatment:Control | Sample.condition:Mitochondria RAW_FILE_NAME=Pex26-MT-NonDerPos-3; RAW_FILE_NAME=Pex26-MT-NonDerNeg-3; RAW_FILE_NAME=Pex26-MT-DerPos-3; RAW_FILE_NAME=Pex26-MT-Faims-3 SUBJECT_SAMPLE_FACTORS - S19 GenoType:Pex26-knockout | Treatment:Control | Sample.condition:Mitochondria RAW_FILE_NAME=Pex26-MT-NonDerPos-4; RAW_FILE_NAME=Pex26-MT-NonDerNeg-4; RAW_FILE_NAME=Pex26-MT-DerPos-4; RAW_FILE_NAME=Pex26-MT-Faims-4 SUBJECT_SAMPLE_FACTORS - S20 GenoType:Pex26-knockout | Treatment:Control | Sample.condition:Mitochondria RAW_FILE_NAME=Pex26-MT-NonDerPos-5; RAW_FILE_NAME=Pex26-MT-NonDerNeg-5; RAW_FILE_NAME=Pex26-MT-DerPos-5; RAW_FILE_NAME=Pex26-MT-Faims-5 SUBJECT_SAMPLE_FACTORS - S21 GenoType:GOLGA5-knockout | Treatment:Control | Sample.condition:Wholecell RAW_FILE_NAME=Golga5-WC-NonDerPos-1; RAW_FILE_NAME=Golga5-WC-NonDerNeg-1; RAW_FILE_NAME=Golga5-WC-DerPos-1; RAW_FILE_NAME=Golga5-WC-Faims-1 SUBJECT_SAMPLE_FACTORS - S22 GenoType:GOLGA5-knockout | Treatment:Control | Sample.condition:Wholecell RAW_FILE_NAME=Golga5-WC-NonDerPos-2; RAW_FILE_NAME=Golga5-WC-NonDerNeg-2; RAW_FILE_NAME=Golga5-WC-DerPos-2; RAW_FILE_NAME=Golga5-WC-Faims-2 SUBJECT_SAMPLE_FACTORS - S23 GenoType:GOLGA5-knockout | Treatment:Control | Sample.condition:Wholecell RAW_FILE_NAME=Golga5-WC-NonDerPos-3; RAW_FILE_NAME=Golga5-WC-NonDerNeg-3; RAW_FILE_NAME=Golga5-WC-DerPos-3; RAW_FILE_NAME=Golga5-WC-Faims-3 SUBJECT_SAMPLE_FACTORS - S24 GenoType:GOLGA5-knockout | Treatment:Control | Sample.condition:Wholecell RAW_FILE_NAME=Golga5-WC-NonDerPos-4; RAW_FILE_NAME=Golga5-WC-NonDerNeg-4; RAW_FILE_NAME=Golga5-WC-DerPos-4; RAW_FILE_NAME=Golga5-WC-Faims-4 SUBJECT_SAMPLE_FACTORS - S25 GenoType:GOLGA5-knockout | Treatment:Control | Sample.condition:Wholecell RAW_FILE_NAME=Golga5-WC-NonDerPos-5; RAW_FILE_NAME=Golga5-WC-NonDerNeg-5; RAW_FILE_NAME=Golga5-WC-DerPos-5; RAW_FILE_NAME=Golga5-WC-Faims-5 SUBJECT_SAMPLE_FACTORS - S26 GenoType:GOLGA5-knockout | Treatment:Control | Sample.condition:Mitochondria RAW_FILE_NAME=Golga5-MT-NonDerPos-1; RAW_FILE_NAME=Golga5-MT-NonDerNeg-1; RAW_FILE_NAME=Golga5-MT-DerPos-1; RAW_FILE_NAME=Golga5-MT-Faims-1 SUBJECT_SAMPLE_FACTORS - S27 GenoType:GOLGA5-knockout | Treatment:Control | Sample.condition:Mitochondria RAW_FILE_NAME=Golga5-MT-NonDerPos-2; RAW_FILE_NAME=Golga5-MT-NonDerNeg-2; RAW_FILE_NAME=Golga5-MT-DerPos-2; RAW_FILE_NAME=Golga5-MT-Faims-2 SUBJECT_SAMPLE_FACTORS - S28 GenoType:GOLGA5-knockout | Treatment:Control | Sample.condition:Mitochondria RAW_FILE_NAME=Golga5-MT-NonDerPos-3; RAW_FILE_NAME=Golga5-MT-NonDerNeg-3; RAW_FILE_NAME=Golga5-MT-DerPos-3; RAW_FILE_NAME=Golga5-MT-Faims-3 SUBJECT_SAMPLE_FACTORS - S29 GenoType:GOLGA5-knockout | Treatment:Control | Sample.condition:Mitochondria RAW_FILE_NAME=Golga5-MT-NonDerPos-4; RAW_FILE_NAME=Golga5-MT-NonDerNeg-4; RAW_FILE_NAME=Golga5-MT-DerPos-4; RAW_FILE_NAME=Golga5-MT-Faims-4 SUBJECT_SAMPLE_FACTORS - S30 GenoType:GOLGA5-knockout | Treatment:Control | Sample.condition:Mitochondria RAW_FILE_NAME=Golga5-MT-NonDerPos-5; RAW_FILE_NAME=Golga5-MT-NonDerNeg-5; RAW_FILE_NAME=Golga5-MT-DerPos-5; RAW_FILE_NAME=Golga5-MT-Faims-5 SUBJECT_SAMPLE_FACTORS - S31 GenoType:GOLGA8M-knockout | Treatment:Control | Sample.condition:Wholecell RAW_FILE_NAME=Golga8m-WC-NonDerPos-1; RAW_FILE_NAME=Golga8m-WC-NonDerNeg-1; RAW_FILE_NAME=Golga8m-WC-DerPos-1; RAW_FILE_NAME=Golga8m-WC-Faims-1 SUBJECT_SAMPLE_FACTORS - S32 GenoType:GOLGA8M-knockout | Treatment:Control | Sample.condition:Wholecell RAW_FILE_NAME=Golga8m-WC-NonDerPos-2; RAW_FILE_NAME=Golga8m-WC-NonDerNeg-2; RAW_FILE_NAME=Golga8m-WC-DerPos-2; RAW_FILE_NAME=Golga8m-WC-Faims-2 SUBJECT_SAMPLE_FACTORS - S33 GenoType:GOLGA8M-knockout | Treatment:Control | Sample.condition:Wholecell RAW_FILE_NAME=Golga8m-WC-NonDerPos-3; RAW_FILE_NAME=Golga8m-WC-NonDerNeg-3; RAW_FILE_NAME=Golga8m-WC-DerPos-3; RAW_FILE_NAME=Golga8m-WC-Faims-3 SUBJECT_SAMPLE_FACTORS - S34 GenoType:GOLGA8M-knockout | Treatment:Control | Sample.condition:Wholecell RAW_FILE_NAME=Golga8m-WC-NonDerPos-4; RAW_FILE_NAME=Golga8m-WC-NonDerNeg-4; RAW_FILE_NAME=Golga8m-WC-DerPos-4; RAW_FILE_NAME=Golga8m-WC-Faims-4 SUBJECT_SAMPLE_FACTORS - S35 GenoType:GOLGA8M-knockout | Treatment:Control | Sample.condition:Wholecell RAW_FILE_NAME=Golga8m-WC-NonDerPos-5; RAW_FILE_NAME=Golga8m-WC-NonDerNeg-5; RAW_FILE_NAME=Golga8m-WC-DerPos-5; RAW_FILE_NAME=Golga8m-WC-Faims-5 SUBJECT_SAMPLE_FACTORS - S36 GenoType:GOLGA8M-knockout | Treatment:Control | Sample.condition:Mitochondria RAW_FILE_NAME=Golga8m-MT-NonDerPos-1; RAW_FILE_NAME=Golga8m-MT-NonDerNeg-1; RAW_FILE_NAME=Golga8m-MT-DerPos-1; RAW_FILE_NAME=Golga8m-MT-Faims-1 SUBJECT_SAMPLE_FACTORS - S37 GenoType:GOLGA8M-knockout | Treatment:Control | Sample.condition:Mitochondria RAW_FILE_NAME=Golga8m-MT-NonDerPos-2; RAW_FILE_NAME=Golga8m-MT-NonDerNeg-2; RAW_FILE_NAME=Golga8m-MT-DerPos-2; RAW_FILE_NAME=Golga8m-MT-Faims-2 SUBJECT_SAMPLE_FACTORS - S38 GenoType:GOLGA8M-knockout | Treatment:Control | Sample.condition:Mitochondria RAW_FILE_NAME=Golga8m-MT-NonDerPos-3; RAW_FILE_NAME=Golga8m-MT-NonDerNeg-3; RAW_FILE_NAME=Golga8m-MT-DerPos-3; RAW_FILE_NAME=Golga8m-MT-Faims-3 SUBJECT_SAMPLE_FACTORS - S39 GenoType:GOLGA8M-knockout | Treatment:Control | Sample.condition:Mitochondria RAW_FILE_NAME=Golga8m-MT-NonDerPos-4; RAW_FILE_NAME=Golga8m-MT-NonDerNeg-4; RAW_FILE_NAME=Golga8m-MT-DerPos-4; RAW_FILE_NAME=Golga8m-MT-Faims-4 SUBJECT_SAMPLE_FACTORS - S40 GenoType:GOLGA8M-knockout | Treatment:Control | Sample.condition:Mitochondria RAW_FILE_NAME=Golga8m-MT-NonDerPos-5; RAW_FILE_NAME=Golga8m-MT-NonDerNeg-5; RAW_FILE_NAME=Golga8m-MT-DerPos-5; RAW_FILE_NAME=Golga8m-MT-Faims-5 SUBJECT_SAMPLE_FACTORS - S41 GenoType:SEC61A2-knockout | Treatment:Control | Sample.condition:Wholecell RAW_FILE_NAME=Sec61a2-WC-NonDerPos-1; RAW_FILE_NAME=Sec61a2-WC-NonDerNeg-1; RAW_FILE_NAME=Sec61a2-WC-DerPos-1; RAW_FILE_NAME=Sec61a2-WC-Faims-1 SUBJECT_SAMPLE_FACTORS - S42 GenoType:SEC61A2-knockout | Treatment:Control | Sample.condition:Wholecell RAW_FILE_NAME=Sec61a2-WC-NonDerPos-2; RAW_FILE_NAME=Sec61a2-WC-NonDerNeg-2; RAW_FILE_NAME=Sec61a2-WC-DerPos-2; RAW_FILE_NAME=Sec61a2-WC-Faims-2 SUBJECT_SAMPLE_FACTORS - S43 GenoType:SEC61A2-knockout | Treatment:Control | Sample.condition:Wholecell RAW_FILE_NAME=Sec61a2-WC-NonDerPos-3; RAW_FILE_NAME=Sec61a2-WC-NonDerNeg-3; RAW_FILE_NAME=Sec61a2-WC-DerPos-3; RAW_FILE_NAME=Sec61a2-WC-Faims-3 SUBJECT_SAMPLE_FACTORS - S44 GenoType:SEC61A2-knockout | Treatment:Control | Sample.condition:Wholecell RAW_FILE_NAME=Sec61a2-WC-NonDerPos-4; RAW_FILE_NAME=Sec61a2-WC-NonDerNeg-4; RAW_FILE_NAME=Sec61a2-WC-DerPos-4; RAW_FILE_NAME=Sec61a2-WC-Faims-4 SUBJECT_SAMPLE_FACTORS - S45 GenoType:SEC61A2-knockout | Treatment:Control | Sample.condition:Wholecell RAW_FILE_NAME=Sec61a2-WC-NonDerPos-5; RAW_FILE_NAME=Sec61a2-WC-NonDerNeg-5; RAW_FILE_NAME=Sec61a2-WC-DerPos-5; RAW_FILE_NAME=Sec61a2-WC-Faims-5 SUBJECT_SAMPLE_FACTORS - S46 GenoType:SEC61A2-knockout | Treatment:Control | Sample.condition:Mitochondria RAW_FILE_NAME=Sec61a2-MT-NonDerPos-1; RAW_FILE_NAME=Sec61a2-MT-NonDerNeg-1; RAW_FILE_NAME=Sec61a2-MT-DerPos-1; RAW_FILE_NAME=Sec61a2-MT-Faims-1 SUBJECT_SAMPLE_FACTORS - S47 GenoType:SEC61A2-knockout | Treatment:Control | Sample.condition:Mitochondria RAW_FILE_NAME=Sec61a2-MT-NonDerPos-2; RAW_FILE_NAME=Sec61a2-MT-NonDerNeg-2; RAW_FILE_NAME=Sec61a2-MT-DerPos-2; RAW_FILE_NAME=Sec61a2-MT-Faims-2 SUBJECT_SAMPLE_FACTORS - S48 GenoType:SEC61A2-knockout | Treatment:Control | Sample.condition:Mitochondria RAW_FILE_NAME=Sec61a2-MT-NonDerPos-3; RAW_FILE_NAME=Sec61a2-MT-NonDerNeg-3; RAW_FILE_NAME=Sec61a2-MT-DerPos-3; RAW_FILE_NAME=Sec61a2-MT-Faims-3 SUBJECT_SAMPLE_FACTORS - S49 GenoType:SEC61A2-knockout | Treatment:Control | Sample.condition:Mitochondria RAW_FILE_NAME=Sec61a2-MT-NonDerPos-4; RAW_FILE_NAME=Sec61a2-MT-NonDerNeg-4; RAW_FILE_NAME=Sec61a2-MT-DerPos-4; RAW_FILE_NAME=Sec61a2-MT-Faims-4 SUBJECT_SAMPLE_FACTORS - S50 GenoType:SEC61A2-knockout | Treatment:Control | Sample.condition:Mitochondria RAW_FILE_NAME=Sec61a2-MT-NonDerPos-5; RAW_FILE_NAME=Sec61a2-MT-NonDerNeg-5; RAW_FILE_NAME=Sec61a2-MT-DerPos-5; RAW_FILE_NAME=Sec61a2-MT-Faims-5 SUBJECT_SAMPLE_FACTORS - S51 GenoType:SEC62-knockout | Treatment:Control | Sample.condition:Wholecell RAW_FILE_NAME=Sec62-WC-NonDerPos-1; RAW_FILE_NAME=Sec62-WC-NonDerNeg-1; RAW_FILE_NAME=Sec62-WC-DerPos-1; RAW_FILE_NAME=Sec62-WC-Faims-1 SUBJECT_SAMPLE_FACTORS - S52 GenoType:SEC62-knockout | Treatment:Control | Sample.condition:Wholecell RAW_FILE_NAME=Sec62-WC-NonDerPos-2; RAW_FILE_NAME=Sec62-WC-NonDerNeg-2; RAW_FILE_NAME=Sec62-WC-DerPos-2; RAW_FILE_NAME=Sec62-WC-Faims-2 SUBJECT_SAMPLE_FACTORS - S53 GenoType:SEC62-knockout | Treatment:Control | Sample.condition:Wholecell RAW_FILE_NAME=Sec62-WC-NonDerPos-3; RAW_FILE_NAME=Sec62-WC-NonDerNeg-3; RAW_FILE_NAME=Sec62-WC-DerPos-3; RAW_FILE_NAME=Sec62-WC-Faims-3 SUBJECT_SAMPLE_FACTORS - S54 GenoType:SEC62-knockout | Treatment:Control | Sample.condition:Wholecell RAW_FILE_NAME=Sec62-WC-NonDerPos-4; RAW_FILE_NAME=Sec62-WC-NonDerNeg-4; RAW_FILE_NAME=Sec62-WC-DerPos-4; RAW_FILE_NAME=Sec62-WC-Faims-4 SUBJECT_SAMPLE_FACTORS - S55 GenoType:SEC62-knockout | Treatment:Control | Sample.condition:Wholecell RAW_FILE_NAME=Sec62-WC-NonDerPos-5; RAW_FILE_NAME=Sec62-WC-NonDerNeg-5; RAW_FILE_NAME=Sec62-WC-DerPos-5; RAW_FILE_NAME=Sec62-WC-Faims-5 SUBJECT_SAMPLE_FACTORS - S56 GenoType:SEC62-knockout | Treatment:Control | Sample.condition:Mitochondria RAW_FILE_NAME=Sec62-MT-NonDerPos-1; RAW_FILE_NAME=Sec62-MT-NonDerNeg-1; RAW_FILE_NAME=Sec62-MT-DerPos-1; RAW_FILE_NAME=Sec62-MT-Faims-1 SUBJECT_SAMPLE_FACTORS - S57 GenoType:SEC62-knockout | Treatment:Control | Sample.condition:Mitochondria RAW_FILE_NAME=Sec62-MT-NonDerPos-2; RAW_FILE_NAME=Sec62-MT-NonDerNeg-2; RAW_FILE_NAME=Sec62-MT-DerPos-2; RAW_FILE_NAME=Sec62-MT-Faims-2 SUBJECT_SAMPLE_FACTORS - S58 GenoType:SEC62-knockout | Treatment:Control | Sample.condition:Mitochondria RAW_FILE_NAME=Sec62-MT-NonDerPos-3; RAW_FILE_NAME=Sec62-MT-NonDerNeg-3; RAW_FILE_NAME=Sec62-MT-DerPos-3; RAW_FILE_NAME=Sec62-MT-Faims-3 SUBJECT_SAMPLE_FACTORS - S59 GenoType:SEC62-knockout | Treatment:Control | Sample.condition:Mitochondria RAW_FILE_NAME=Sec62-MT-NonDerPos-4; RAW_FILE_NAME=Sec62-MT-NonDerNeg-4; RAW_FILE_NAME=Sec62-MT-DerPos-4; RAW_FILE_NAME=Sec62-MT-Faims-4 SUBJECT_SAMPLE_FACTORS - S60 GenoType:SEC62-knockout | Treatment:Control | Sample.condition:Mitochondria RAW_FILE_NAME=Sec62-MT-NonDerPos-5; RAW_FILE_NAME=Sec62-MT-NonDerNeg-5; RAW_FILE_NAME=Sec62-MT-DerPos-5; RAW_FILE_NAME=Sec62-MT-Faims-5 SUBJECT_SAMPLE_FACTORS - S61 GenoType:SEC61A2-knockout | Treatment:Vehicle | Sample.condition:wholecell RAW_FILE_NAME=Sec61a2-V-WC-1_NonDerPos; RAW_FILE_NAME=Sec61a2-V-WC-1_NonDerNeg; RAW_FILE_NAME=Der_Pos 5in40_Sec61_e1; RAW_FILE_NAME=LT01JB1T1-Sample__Sec61E--B1_8X_MS_LP_230220_FAIMS_Neg_1 SUBJECT_SAMPLE_FACTORS - S62 GenoType:SEC61A2-knockout | Treatment:Vehicle | Sample.condition:wholecell RAW_FILE_NAME=Sec61a2-V-WC-2_NonDerPos; RAW_FILE_NAME=Sec61a2-V-WC-2_NonDerNeg; RAW_FILE_NAME=Der_Pos 5in40_Sec61_e2; RAW_FILE_NAME=LT01JB2T1-Sample__Sec61E--B2_8X_MS_LP_230220_FAIMS_Neg_1 SUBJECT_SAMPLE_FACTORS - S63 GenoType:SEC61A2-knockout | Treatment:Vehicle | Sample.condition:wholecell RAW_FILE_NAME=Sec61a2-V-WC-3_NonDerPos; RAW_FILE_NAME=Sec61a2-V-WC-3_NonDerNeg; RAW_FILE_NAME=Der_Pos 5in40_Sec61_e3; RAW_FILE_NAME=LT01JB3T1-Sample__Sec61E--B3_8X_MS_LP_230220_FAIMS_Neg_1 SUBJECT_SAMPLE_FACTORS - S64 GenoType:SEC61A2-knockout | Treatment:Vehicle | Sample.condition:wholecell RAW_FILE_NAME=Sec61a2-V-WC-4_NonDerPos; RAW_FILE_NAME=Sec61a2-V-WC-4_NonDerNeg; RAW_FILE_NAME=Der_Pos 5in40_Sec61_e4; RAW_FILE_NAME=LT01JB4T1-Sample__Sec61E--B4_8X_MS_LP_230220_FAIMS_Neg_1 SUBJECT_SAMPLE_FACTORS - S65 GenoType:SEC61A2-knockout | Treatment:Vehicle | Sample.condition:wholecell RAW_FILE_NAME=Sec61a2-V-WC-5_NonDerPos; RAW_FILE_NAME=Sec61a2-V-WC-5_NonDerNeg; RAW_FILE_NAME=Der_Pos 5in40_Sec61_e5; RAW_FILE_NAME=LT01JB5T1-Sample__Sec61E--B5_8X_MS_LP_230220_FAIMS_Neg_1 SUBJECT_SAMPLE_FACTORS - S66 GenoType:SEC61A2-knockout | Treatment:1-O-Octadecyl-sn-glycerol | Sample.condition:Wholecell RAW_FILE_NAME=Sec61a2-Oct-WC-1_NonDerPos; RAW_FILE_NAME=Sec61a2-Oct-WC-1_NonDerNeg; RAW_FILE_NAME=Der_Pos 5in40_Sec61_o1; RAW_FILE_NAME=LT01LB1T1-Sample__Sec61O--B1_8X_MS_LP_230220_FAIMS_Neg_1 SUBJECT_SAMPLE_FACTORS - S67 GenoType:SEC61A2-knockout | Treatment:1-O-Octadecyl-sn-glycerol | Sample.condition:Wholecell RAW_FILE_NAME=Sec61a2-Oct-WC-2_NonDerPos; RAW_FILE_NAME=Sec61a2-Oct-WC-2_NonDerNeg; RAW_FILE_NAME=Der_Pos 5in40_Sec61_o2; RAW_FILE_NAME=LT01LB2T1-Sample__Sec61O--B2_8X_MS_LP_230220_FAIMS_Neg_1 SUBJECT_SAMPLE_FACTORS - S68 GenoType:SEC61A2-knockout | Treatment:1-O-Octadecyl-sn-glycerol | Sample.condition:Wholecell RAW_FILE_NAME=Sec61a2-Oct-WC-3_NonDerPos; RAW_FILE_NAME=Sec61a2-Oct-WC-3_NonDerNeg; RAW_FILE_NAME=Der_Pos 5in40_Sec61_o3; RAW_FILE_NAME=LT01LB3T1-Sample__Sec61O--B3_8X_MS_LP_230220_FAIMS_Neg_1 SUBJECT_SAMPLE_FACTORS - S69 GenoType:SEC61A2-knockout | Treatment:1-O-Octadecyl-sn-glycerol | Sample.condition:Wholecell RAW_FILE_NAME=Sec61a2-Oct-WC-4_NonDerPos; RAW_FILE_NAME=Sec61a2-Oct-WC-4_NonDerNeg; RAW_FILE_NAME=Der_Pos 5in40_Sec61_o4; RAW_FILE_NAME=LT01LB4T1-Sample__Sec61O--B4_8X_MS_LP_230220_FAIMS_Neg_1 SUBJECT_SAMPLE_FACTORS - S70 GenoType:SEC61A2-knockout | Treatment:1-O-Octadecyl-sn-glycerol | Sample.condition:Wholecell RAW_FILE_NAME=Sec61a2-Oct-WC-5_NonDerPos; RAW_FILE_NAME=Sec61a2-Oct-WC-5_NonDerNeg; RAW_FILE_NAME=Der_Pos 5in40_Sec61_o5; RAW_FILE_NAME=LT01LB5T1-Sample__Sec61O--B5_8X_MS_LP_230220_FAIMS_Neg_1 SUBJECT_SAMPLE_FACTORS - S71 GenoType:SEC61A2-knockout | Treatment:1-O-Hexadecyl-sn-glycerol | Sample.condition:Wholecell RAW_FILE_NAME=Sec61a2-Hex-WC-1_NonDerPos; RAW_FILE_NAME=Sec61a2-Hex-WC-1_NonDerNeg; RAW_FILE_NAME=Der_Pos 5in40_Sec61_h1; RAW_FILE_NAME=LT01KB1T1-Sample__Sec61H--B1_8X_MS_LP_230220_FAIMS_Neg_1 SUBJECT_SAMPLE_FACTORS - S72 GenoType:SEC61A2-knockout | Treatment:1-O-Hexadecyl-sn-glycerol | Sample.condition:Wholecell RAW_FILE_NAME=Sec61a2-Hex-WC-2_NonDerPos; RAW_FILE_NAME=Sec61a2-Hex-WC-2_NonDerNeg; RAW_FILE_NAME=Der_Pos 5in40_Sec61_h2; RAW_FILE_NAME=LT01KB2T1-Sample__Sec61H--B2_8X_MS_LP_230220_FAIMS_Neg_1 SUBJECT_SAMPLE_FACTORS - S73 GenoType:SEC61A2-knockout | Treatment:1-O-Hexadecyl-sn-glycerol | Sample.condition:Wholecell RAW_FILE_NAME=Sec61a2-Hex-WC-3_NonDerPos; RAW_FILE_NAME=Sec61a2-Hex-WC-3_NonDerNeg; RAW_FILE_NAME=Der_Pos 5in40_Sec61_h3; RAW_FILE_NAME=LT01KB3T1-Sample__Sec61H--B3_8X_MS_LP_230220_FAIMS_Neg_1 SUBJECT_SAMPLE_FACTORS - S74 GenoType:SEC61A2-knockout | Treatment:1-O-Hexadecyl-sn-glycerol | Sample.condition:Wholecell RAW_FILE_NAME=Sec61a2-Hex-WC-4_NonDerPos; RAW_FILE_NAME=Sec61a2-Hex-WC-4_NonDerNeg; RAW_FILE_NAME=Der_Pos 5in40_Sec61_h4; RAW_FILE_NAME=LT01KB4T1-Sample__Sec61H--B4_8X_MS_LP_230220_FAIMS_Neg_1 SUBJECT_SAMPLE_FACTORS - S75 GenoType:SEC61A2-knockout | Treatment:1-O-Hexadecyl-sn-glycerol | Sample.condition:Wholecell RAW_FILE_NAME=Sec61a2-Hex-WC-5_NonDerPos; RAW_FILE_NAME=Sec61a2-Hex-WC-5_NonDerNeg; RAW_FILE_NAME=Der_Pos 5in40_Sec61_h5; RAW_FILE_NAME=LT01KB5T1-Sample__Sec61H--B5_8X_MS_LP_230220_FAIMS_Neg_1 SUBJECT_SAMPLE_FACTORS - S76 GenoType:SEC62-knockout | Treatment:Vehicle | Sample.condition:wholecell RAW_FILE_NAME=Sec62-V-WC-1_NonDerPos; RAW_FILE_NAME=Sec62-V-WC-1_NonDerNeg; RAW_FILE_NAME=Der_Pos 5in40_Sec62_e1; RAW_FILE_NAME=LT01MB1T1-Sample__Sec62E--B1_8X_MS_LP_230220_FAIMS_Neg_1 SUBJECT_SAMPLE_FACTORS - S77 GenoType:SEC62-knockout | Treatment:Vehicle | Sample.condition:wholecell RAW_FILE_NAME=Sec62-V-WC-2_NonDerPos; RAW_FILE_NAME=Sec62-V-WC-2_NonDerNeg; RAW_FILE_NAME=Der_Pos 5in40_Sec62_e2; RAW_FILE_NAME=LT01MB2T1-Sample__Sec62E--B2_8X_MS_LP_230220_FAIMS_Neg_1 SUBJECT_SAMPLE_FACTORS - S78 GenoType:SEC62-knockout | Treatment:Vehicle | Sample.condition:wholecell RAW_FILE_NAME=Sec62-V-WC-3_NonDerPos; RAW_FILE_NAME=Sec62-V-WC-3_NonDerNeg; RAW_FILE_NAME=Der_Pos 5in40_Sec62_e3; RAW_FILE_NAME=LT01MB3T1-Sample__Sec62E--B3_8X_MS_LP_230220_FAIMS_Neg_1 SUBJECT_SAMPLE_FACTORS - S79 GenoType:SEC62-knockout | Treatment:Vehicle | Sample.condition:wholecell RAW_FILE_NAME=Sec62-V-WC-4_NonDerPos; RAW_FILE_NAME=Sec62-V-WC-4_NonDerNeg; RAW_FILE_NAME=Der_Pos 5in40_Sec62_e4; RAW_FILE_NAME=LT01MB4T1-Sample__Sec62E--B4_8X_MS_LP_230220_FAIMS_Neg_1 SUBJECT_SAMPLE_FACTORS - S80 GenoType:SEC62-knockout | Treatment:Vehicle | Sample.condition:wholecell RAW_FILE_NAME=Sec62-V-WC-5_NonDerPos; RAW_FILE_NAME=Sec62-V-WC-5_NonDerNeg; RAW_FILE_NAME=Der_Pos 5in40_Sec62_e5; RAW_FILE_NAME=LT01MB5T1-Sample__Sec62E--B5_8X_MS_LP_230220_FAIMS_Neg_1 SUBJECT_SAMPLE_FACTORS - S81 GenoType:SEC62-knockout | Treatment:1-O-Octadecyl-sn-glycerol | Sample.condition:Wholecell RAW_FILE_NAME=Sec62-Oct-WC-1_NonDerPos; RAW_FILE_NAME=Sec62-Oct-WC-1_NonDerNeg; RAW_FILE_NAME=Der_Pos 5in40_Sec62_o1; RAW_FILE_NAME=LT01OB1T1-Sample__Sec62O--B1_8X_MS_LP_230220_FAIMS_Neg_1 SUBJECT_SAMPLE_FACTORS - S82 GenoType:SEC62-knockout | Treatment:1-O-Octadecyl-sn-glycerol | Sample.condition:Wholecell RAW_FILE_NAME=Sec62-Oct-WC-2_NonDerPos; RAW_FILE_NAME=Sec62-Oct-WC-2_NonDerNeg; RAW_FILE_NAME=Der_Pos 5in40_Sec62_o2; RAW_FILE_NAME=LT01OB2T1-Sample__Sec62O--B2_8X_MS_LP_230220_FAIMS_Neg_1 SUBJECT_SAMPLE_FACTORS - S83 GenoType:SEC62-knockout | Treatment:1-O-Octadecyl-sn-glycerol | Sample.condition:Wholecell RAW_FILE_NAME=Sec62-Oct-WC-3_NonDerPos; RAW_FILE_NAME=Sec62-Oct-WC-3_NonDerNeg; RAW_FILE_NAME=Der_Pos 5in40_Sec62_o3; RAW_FILE_NAME=LT01OB3T1-Sample__Sec62O--B3_8X_MS_LP_230220_FAIMS_Neg_1 SUBJECT_SAMPLE_FACTORS - S84 GenoType:SEC62-knockout | Treatment:1-O-Octadecyl-sn-glycerol | Sample.condition:Wholecell RAW_FILE_NAME=Sec62-Oct-WC-4_NonDerPos; RAW_FILE_NAME=Sec62-Oct-WC-4_NonDerNeg; RAW_FILE_NAME=Der_Pos 5in40_Sec62_o4; RAW_FILE_NAME=LT01OB4T1-Sample__Sec62O--B4_8X_MS_LP_230220_FAIMS_Neg_1 SUBJECT_SAMPLE_FACTORS - S85 GenoType:SEC62-knockout | Treatment:1-O-Octadecyl-sn-glycerol | Sample.condition:Wholecell RAW_FILE_NAME=Sec62-Oct-WC-5_NonDerPos; RAW_FILE_NAME=Sec62-Oct-WC-5_NonDerNeg; RAW_FILE_NAME=Der_Pos 5in40_Sec62_o5; RAW_FILE_NAME=LT01OB5T1-Sample__Sec62O--B5_8X_MS_LP_230220_FAIMS_Neg_1 SUBJECT_SAMPLE_FACTORS - S86 GenoType:SEC62-knockout | Treatment:1-O-Hexadecyl-sn-glycerol | Sample.condition:Wholecell RAW_FILE_NAME=Sec62-Hex-WC-1_NonDerPos; RAW_FILE_NAME=Sec62-Hex-WC-1_NonDerNeg; RAW_FILE_NAME=Der_Pos 5in40_Sec62_h1; RAW_FILE_NAME=LT01NB1T1-Sample__Sec62H--B1_8X_MS_LP_230220_FAIMS_Neg_1 SUBJECT_SAMPLE_FACTORS - S87 GenoType:SEC62-knockout | Treatment:1-O-Hexadecyl-sn-glycerol | Sample.condition:Wholecell RAW_FILE_NAME=Sec62-Hex-WC-2_NonDerPos; RAW_FILE_NAME=Sec62-Hex-WC-2_NonDerNeg; RAW_FILE_NAME=Der_Pos 5in40_Sec62_h2; RAW_FILE_NAME=LT01NB2T1-Sample__Sec62H--B2_8X_MS_LP_230220_FAIMS_Neg_1 SUBJECT_SAMPLE_FACTORS - S88 GenoType:SEC62-knockout | Treatment:1-O-Hexadecyl-sn-glycerol | Sample.condition:Wholecell RAW_FILE_NAME=Sec62-Hex-WC-3_NonDerPos; RAW_FILE_NAME=Sec62-Hex-WC-3_NonDerNeg; RAW_FILE_NAME=Der_Pos 5in40_Sec62_h3; RAW_FILE_NAME=LT01NB3T1-Sample__Sec62H--B3_8X_MS_LP_230220_FAIMS_Neg_1 SUBJECT_SAMPLE_FACTORS - S89 GenoType:SEC62-knockout | Treatment:1-O-Hexadecyl-sn-glycerol | Sample.condition:Wholecell RAW_FILE_NAME=Sec62-Hex-WC-4_NonDerPos; RAW_FILE_NAME=Sec62-Hex-WC-4_NonDerNeg; RAW_FILE_NAME=Der_Pos 5in40_Sec62_h4; RAW_FILE_NAME=LT01NB4T1-Sample__Sec62H--B4_8X_MS_LP_230220_FAIMS_Neg_1 SUBJECT_SAMPLE_FACTORS - S90 GenoType:SEC62-knockout | Treatment:1-O-Hexadecyl-sn-glycerol | Sample.condition:Wholecell RAW_FILE_NAME=Sec62-Hex-WC-5_NonDerPos; RAW_FILE_NAME=Sec62-Hex-WC-5_NonDerNeg; RAW_FILE_NAME=Der_Pos 5in40_Sec62_h5; RAW_FILE_NAME=LT01NB5T1-Sample__Sec62H--B5_8X_MS_LP_230220_FAIMS_Neg_1 SUBJECT_SAMPLE_FACTORS - S91 GenoType:GOLGA8M-knockout | Treatment:Vehicle | Sample.condition:wholecell RAW_FILE_NAME=Golga8m-V-WC-1_NonDerPos; RAW_FILE_NAME=Golga8m-V-WC-1_NonDerNeg; RAW_FILE_NAME=Der_Pos 5in40_G8M_e1; RAW_FILE_NAME=LT01DB1T1-Sample__G8ME--B1_8X_MS_LP_230220_FAIMS_Neg_1 SUBJECT_SAMPLE_FACTORS - S92 GenoType:GOLGA8M-knockout | Treatment:Vehicle | Sample.condition:wholecell RAW_FILE_NAME=Golga8m-V-WC-2_NonDerPos; RAW_FILE_NAME=Golga8m-V-WC-2_NonDerNeg; RAW_FILE_NAME=Der_Pos 5in40_G8M_e2; RAW_FILE_NAME=LT01DB2T1-Sample__G8ME--B2_8X_MS_LP_230220_FAIMS_Neg_1 SUBJECT_SAMPLE_FACTORS - S93 GenoType:GOLGA8M-knockout | Treatment:Vehicle | Sample.condition:wholecell RAW_FILE_NAME=Golga8m-V-WC-3_NonDerPos; RAW_FILE_NAME=Golga8m-V-WC-3_NonDerNeg; RAW_FILE_NAME=Der_Pos 5in40_G8M_e3; RAW_FILE_NAME=LT01DB3T1-Sample__G8ME--B3_8X_MS_LP_230220_FAIMS_Neg_1 SUBJECT_SAMPLE_FACTORS - S94 GenoType:GOLGA8M-knockout | Treatment:Vehicle | Sample.condition:wholecell RAW_FILE_NAME=Golga8m-V-WC-4_NonDerPos; RAW_FILE_NAME=Golga8m-V-WC-4_NonDerNeg; RAW_FILE_NAME=Der_Pos 5in40_G8M_e4; RAW_FILE_NAME=LT01DB4T1-Sample__G8ME--B4_8X_MS_LP_230220_FAIMS_Neg_1 SUBJECT_SAMPLE_FACTORS - S95 GenoType:GOLGA8M-knockout | Treatment:Vehicle | Sample.condition:wholecell RAW_FILE_NAME=Golga8m-V-WC-5_NonDerPos; RAW_FILE_NAME=Golga8m-V-WC-5_NonDerNeg; RAW_FILE_NAME=Der_Pos 5in40_G8M_e5; RAW_FILE_NAME=LT01DB5T1-Sample__G8ME--B5_8X_MS_LP_230220_FAIMS_Neg_1 SUBJECT_SAMPLE_FACTORS - S96 GenoType:GOLGA8M-knockout | Treatment:1-O-Octadecyl-sn-glycerol | Sample.condition:Wholecell RAW_FILE_NAME=Golga8m-Oct-WC-1_NonDerPos; RAW_FILE_NAME=Golga8m-Oct-WC-1_NonDerNeg; RAW_FILE_NAME=Der_Pos 5in40_G8M_o1; RAW_FILE_NAME=LT01FB1T1-Sample__G8MO--B1_8X_MS_LP_230220_FAIMS_Neg_1 SUBJECT_SAMPLE_FACTORS - S97 GenoType:GOLGA8M-knockout | Treatment:1-O-Octadecyl-sn-glycerol | Sample.condition:Wholecell RAW_FILE_NAME=Golga8m-Oct-WC-2_NonDerPos; RAW_FILE_NAME=Golga8m-Oct-WC-2_NonDerNeg; RAW_FILE_NAME=Der_Pos 5in40_G8M_o2; RAW_FILE_NAME=LT01FB2T1-Sample__G8MO--B2_8X_MS_LP_230220_FAIMS_Neg_1 SUBJECT_SAMPLE_FACTORS - S98 GenoType:GOLGA8M-knockout | Treatment:1-O-Octadecyl-sn-glycerol | Sample.condition:Wholecell RAW_FILE_NAME=Golga8m-Oct-WC-3_NonDerPos; RAW_FILE_NAME=Golga8m-Oct-WC-3_NonDerNeg; RAW_FILE_NAME=Der_Pos 5in40_G8M_o3; RAW_FILE_NAME=LT01FB3T1-Sample__G8MO--B3_8X_MS_LP_230220_FAIMS_Neg_1 SUBJECT_SAMPLE_FACTORS - S99 GenoType:GOLGA8M-knockout | Treatment:1-O-Octadecyl-sn-glycerol | Sample.condition:Wholecell RAW_FILE_NAME=Golga8m-Oct-WC-4_NonDerPos; RAW_FILE_NAME=Golga8m-Oct-WC-4_NonDerNeg; RAW_FILE_NAME=Der_Pos 5in40_G8M_o4; RAW_FILE_NAME=LT01FB4T1-Sample__G8MO--B4_8X_MS_LP_230220_FAIMS_Neg_1 SUBJECT_SAMPLE_FACTORS - S100 GenoType:GOLGA8M-knockout | Treatment:1-O-Octadecyl-sn-glycerol | Sample.condition:Wholecell RAW_FILE_NAME=Golga8m-Oct-WC-5_NonDerPos; RAW_FILE_NAME=Golga8m-Oct-WC-5_NonDerNeg; RAW_FILE_NAME=Der_Pos 5in40_G8M_o5; RAW_FILE_NAME=LT01FB5T1-Sample__G8MO--B5_8X_MS_LP_230220_FAIMS_Neg_1 SUBJECT_SAMPLE_FACTORS - S101 GenoType:GOLGA8M-knockout | Treatment:1-O-Hexadecyl-sn-glycerol | Sample.condition:Wholecell RAW_FILE_NAME=Golga8m-Hex-WC-1_NonDerPos; RAW_FILE_NAME=Golga8m-Hex-WC-1_NonDerNeg; RAW_FILE_NAME=Der_Pos 5in40_G8M_h1; RAW_FILE_NAME=LT01EB1T1-Sample__G8MH--B1_8X_MS_LP_230220_FAIMS_Neg_1 SUBJECT_SAMPLE_FACTORS - S102 GenoType:GOLGA8M-knockout | Treatment:1-O-Hexadecyl-sn-glycerol | Sample.condition:Wholecell RAW_FILE_NAME=Golga8m-Hex-WC-2_NonDerPos; RAW_FILE_NAME=Golga8m-Hex-WC-2_NonDerNeg; RAW_FILE_NAME=Der_Pos 5in40_G8M_h2; RAW_FILE_NAME=LT01EB2T1-Sample__G8MH--B2_8X_MS_LP_230220_FAIMS_Neg_1 SUBJECT_SAMPLE_FACTORS - S103 GenoType:GOLGA8M-knockout | Treatment:1-O-Hexadecyl-sn-glycerol | Sample.condition:Wholecell RAW_FILE_NAME=Golga8m-Hex-WC-3_NonDerPos; RAW_FILE_NAME=Golga8m-Hex-WC-3_NonDerNeg; RAW_FILE_NAME=Der_Pos 5in40_G8M_h3; RAW_FILE_NAME=LT01EB3T1-Sample__G8MH--B3_8X_MS_LP_230220_FAIMS_Neg_1 SUBJECT_SAMPLE_FACTORS - S104 GenoType:GOLGA8M-knockout | Treatment:1-O-Hexadecyl-sn-glycerol | Sample.condition:Wholecell RAW_FILE_NAME=Golga8m-Hex-WC-4_NonDerPos; RAW_FILE_NAME=Golga8m-Hex-WC-4_NonDerNeg; RAW_FILE_NAME=Der_Pos 5in40_G8M_h4; RAW_FILE_NAME=LT01EB4T1-Sample__G8MH--B4_8X_MS_LP_230220_FAIMS_Neg_1 SUBJECT_SAMPLE_FACTORS - S105 GenoType:GOLGA8M-knockout | Treatment:1-O-Hexadecyl-sn-glycerol | Sample.condition:Wholecell RAW_FILE_NAME=Golga8m-Hex-WC-5_NonDerPos; RAW_FILE_NAME=Golga8m-Hex-WC-5_NonDerNeg; RAW_FILE_NAME=Der_Pos 5in40_G8M_h5; RAW_FILE_NAME=LT01EB5T1-Sample__G8MH--B5_8X_MS_LP_230220_FAIMS_Neg_1 SUBJECT_SAMPLE_FACTORS - S106 GenoType:Wild-type | Treatment:Vehicle | Sample.condition:wholecell RAW_FILE_NAME=WT-V-WC-1_NonDerPos; RAW_FILE_NAME=WT-V-WC-1_NonDerNeg; RAW_FILE_NAME=Der_Pos 5in40_WT_e1; RAW_FILE_NAME=LT01PB1T1-Sample__WTE--B1_8X_MS_LP_230220_FAIMS_Neg_1 SUBJECT_SAMPLE_FACTORS - S107 GenoType:Wild-type | Treatment:Vehicle | Sample.condition:wholecell RAW_FILE_NAME=WT-V-WC-2_NonDerPos; RAW_FILE_NAME=WT-V-WC-2_NonDerNeg; RAW_FILE_NAME=Der_Pos 5in40_WT_e2; RAW_FILE_NAME=LT01PB2T1-Sample__WTE--B2_8X_MS_LP_230220_FAIMS_Neg_1 SUBJECT_SAMPLE_FACTORS - S108 GenoType:Wild-type | Treatment:Vehicle | Sample.condition:wholecell RAW_FILE_NAME=WT-V-WC-3_NonDerPos; RAW_FILE_NAME=WT-V-WC-3_NonDerNeg; RAW_FILE_NAME=Der_Pos 5in40_WT_e3; RAW_FILE_NAME=LT01PB3T1-Sample__WTE--B3_8X_MS_LP_230220_FAIMS_Neg_1 SUBJECT_SAMPLE_FACTORS - S109 GenoType:Wild-type | Treatment:Vehicle | Sample.condition:wholecell RAW_FILE_NAME=WT-V-WC-4_NonDerPos; RAW_FILE_NAME=WT-V-WC-4_NonDerNeg; RAW_FILE_NAME=Der_Pos 5in40_WT_e4; RAW_FILE_NAME=LT01PB4T1-Sample__WTE--B4_8X_MS_LP_230220_FAIMS_Neg_1 SUBJECT_SAMPLE_FACTORS - S110 GenoType:Wild-type | Treatment:Vehicle | Sample.condition:wholecell RAW_FILE_NAME=WT-V-WC-5_NonDerPos; RAW_FILE_NAME=WT-V-WC-5_NonDerNeg; RAW_FILE_NAME=Der_Pos 5in40_WT_e5; RAW_FILE_NAME=LT01PB5T1-Sample__WTE--B5_8X_MS_LP_230220_FAIMS_Neg_1 SUBJECT_SAMPLE_FACTORS - S111 GenoType:Wild-type | Treatment:1-O-Octadecyl-sn-glycerol | Sample.condition:Wholecell RAW_FILE_NAME=WT-Oct-WC-1_NonDerPos; RAW_FILE_NAME=WT-Oct-WC-1_NonDerNeg; RAW_FILE_NAME=Der_Pos 5in40_WT_o1; RAW_FILE_NAME=LT01RB1T1-Sample__WTO--B1_8X_MS_LP_230220_FAIMS_Neg_1 SUBJECT_SAMPLE_FACTORS - S112 GenoType:Wild-type | Treatment:1-O-Octadecyl-sn-glycerol | Sample.condition:Wholecell RAW_FILE_NAME=WT-Oct-WC-2_NonDerPos; RAW_FILE_NAME=WT-Oct-WC-2_NonDerNeg; RAW_FILE_NAME=Der_Pos 5in40_WT_o2; RAW_FILE_NAME=LT01RB2T1-Sample__WTO--B2_8X_MS_LP_230220_FAIMS_Neg_1 SUBJECT_SAMPLE_FACTORS - S113 GenoType:Wild-type | Treatment:1-O-Octadecyl-sn-glycerol | Sample.condition:Wholecell RAW_FILE_NAME=WT-Oct-WC-3_NonDerPos; RAW_FILE_NAME=WT-Oct-WC-3_NonDerNeg; RAW_FILE_NAME=Der_Pos 5in40_WT_o3; RAW_FILE_NAME=LT01RB3T1-Sample__WTO--B3_8X_MS_LP_230220_FAIMS_Neg_1 SUBJECT_SAMPLE_FACTORS - S114 GenoType:Wild-type | Treatment:1-O-Octadecyl-sn-glycerol | Sample.condition:Wholecell RAW_FILE_NAME=WT-Oct-WC-4_NonDerPos; RAW_FILE_NAME=WT-Oct-WC-4_NonDerNeg; RAW_FILE_NAME=Der_Pos 5in40_WT_o4; RAW_FILE_NAME=LT01RB4T1-Sample__WTO--B4_8X_MS_LP_230220_FAIMS_Neg_1 SUBJECT_SAMPLE_FACTORS - S115 GenoType:Wild-type | Treatment:1-O-Octadecyl-sn-glycerol | Sample.condition:Wholecell RAW_FILE_NAME=WT-Oct-WC-5_NonDerPos; RAW_FILE_NAME=WT-Oct-WC-5_NonDerNeg; RAW_FILE_NAME=Der_Pos 5in40_WT_o5; RAW_FILE_NAME=LT01RB5T1-Sample__WTO--B5_8X_MS_LP_230220_FAIMS_Neg_1 SUBJECT_SAMPLE_FACTORS - S116 GenoType:Wild-type | Treatment:1-O-Hexadecyl-sn-glycerol | Sample.condition:Wholecell RAW_FILE_NAME=WT-Hex-WC-1_NonDerPos; RAW_FILE_NAME=WT-Hex-WC-1_NonDerNeg; RAW_FILE_NAME=Der_Pos 5in40_WT_h1; RAW_FILE_NAME=LT01QB1T1-Sample__WTH--B1_8X_MS_LP_230220_FAIMS_Neg_1 SUBJECT_SAMPLE_FACTORS - S117 GenoType:Wild-type | Treatment:1-O-Hexadecyl-sn-glycerol | Sample.condition:Wholecell RAW_FILE_NAME=WT-Hex-WC-2_NonDerPos; RAW_FILE_NAME=WT-Hex-WC-2_NonDerNeg; RAW_FILE_NAME=Der_Pos 5in40_WT_h2; RAW_FILE_NAME=LT01QB2T1-Sample__WTH--B2_8X_MS_LP_230220_FAIMS_Neg_1 SUBJECT_SAMPLE_FACTORS - S118 GenoType:Wild-type | Treatment:1-O-Hexadecyl-sn-glycerol | Sample.condition:Wholecell RAW_FILE_NAME=WT-Hex-WC-3_NonDerPos; RAW_FILE_NAME=WT-Hex-WC-3_NonDerNeg; RAW_FILE_NAME=Der_Pos 5in40_WT_h3; RAW_FILE_NAME=LT01QB3T1-Sample__WTH--B3_8X_MS_LP_230220_FAIMS_Neg_1 SUBJECT_SAMPLE_FACTORS - S119 GenoType:Wild-type | Treatment:1-O-Hexadecyl-sn-glycerol | Sample.condition:Wholecell RAW_FILE_NAME=WT-Hex-WC-4_NonDerPos; RAW_FILE_NAME=WT-Hex-WC-4_NonDerNeg; RAW_FILE_NAME=Der_Pos 5in40_WT_h4; RAW_FILE_NAME=LT01QB4T1-Sample__WTH--B4_8X_MS_LP_230220_FAIMS_Neg_1 SUBJECT_SAMPLE_FACTORS - S120 GenoType:Wild-type | Treatment:1-O-Hexadecyl-sn-glycerol | Sample.condition:Wholecell RAW_FILE_NAME=WT-Hex-WC-5_NonDerPos; RAW_FILE_NAME=WT-Hex-WC-5_NonDerNeg; RAW_FILE_NAME=Der_Pos 5in40_WT_h5; RAW_FILE_NAME=LT01QB5T1-Sample__WTH--B5_8X_MS_LP_230220_FAIMS_Neg_1 SUBJECT_SAMPLE_FACTORS - S121 GenoType:GOLGA5-knockout | Treatment:Vehicle | Sample.condition:wholecell RAW_FILE_NAME=Golga5-V-WC-1_NonDerPos; RAW_FILE_NAME=Golga5-V-WC-1_NonDerNeg; RAW_FILE_NAME=Der_Pos 5in40_G5A_e1; RAW_FILE_NAME=LT01AB1T1-Sample__G5AE--B1_8X_MS_LP_230220_FAIMS_Neg_1 SUBJECT_SAMPLE_FACTORS - S122 GenoType:GOLGA5-knockout | Treatment:Vehicle | Sample.condition:wholecell RAW_FILE_NAME=Golga5-V-WC-2_NonDerPos; RAW_FILE_NAME=Golga5-V-WC-2_NonDerNeg; RAW_FILE_NAME=Der_Pos 5in40_G5A_e2; RAW_FILE_NAME=LT01AB2T1-Sample__G5AE--B2_8X_MS_LP_230220_FAIMS_Neg_1 SUBJECT_SAMPLE_FACTORS - S123 GenoType:GOLGA5-knockout | Treatment:Vehicle | Sample.condition:wholecell RAW_FILE_NAME=Golga5-V-WC-3_NonDerPos; RAW_FILE_NAME=Golga5-V-WC-3_NonDerNeg; RAW_FILE_NAME=Der_Pos 5in40_G5A_e3; RAW_FILE_NAME=LT01AB3T1-Sample__G5AE--B3_8X_MS_LP_230220_FAIMS_Neg_1 SUBJECT_SAMPLE_FACTORS - S124 GenoType:GOLGA5-knockout | Treatment:Vehicle | Sample.condition:wholecell RAW_FILE_NAME=Golga5-V-WC-4_NonDerPos; RAW_FILE_NAME=Golga5-V-WC-4_NonDerNeg; RAW_FILE_NAME=Der_Pos 5in40_G5A_e4; RAW_FILE_NAME=LT01AB4T1-Sample__G5AE--B4_8X_MS_LP_230220_FAIMS_Neg_1 SUBJECT_SAMPLE_FACTORS - S125 GenoType:GOLGA5-knockout | Treatment:Vehicle | Sample.condition:wholecell RAW_FILE_NAME=Golga5-V-WC-5_NonDerPos; RAW_FILE_NAME=Golga5-V-WC-5_NonDerNeg; RAW_FILE_NAME=Der_Pos 5in40_G5A_e5; RAW_FILE_NAME=LT01AB5T1-Sample__G5AE--B5_8X_MS_LP_230220_FAIMS_Neg_1 SUBJECT_SAMPLE_FACTORS - S126 GenoType:GOLGA5-knockout | Treatment:1-O-Octadecyl-sn-glycerol | Sample.condition:Wholecell RAW_FILE_NAME=Golga5-Oct-WC-1_NonDerPos; RAW_FILE_NAME=Golga5-Oct-WC-1_NonDerNeg; RAW_FILE_NAME=Der_Pos 5in40_G5A_o1; RAW_FILE_NAME=LT01CB1T1-Sample__G5AO--B1_8X_MS_LP_230220_FAIMS_Neg_1 SUBJECT_SAMPLE_FACTORS - S127 GenoType:GOLGA5-knockout | Treatment:1-O-Octadecyl-sn-glycerol | Sample.condition:Wholecell RAW_FILE_NAME=Golga5-Oct-WC-2_NonDerPos; RAW_FILE_NAME=Golga5-Oct-WC-2_NonDerNeg; RAW_FILE_NAME=Der_Pos 5in40_G5A_o2; RAW_FILE_NAME=LT01CB2T1-Sample__G5AO--B2_8X_MS_LP_230220_FAIMS_Neg_1 SUBJECT_SAMPLE_FACTORS - S128 GenoType:GOLGA5-knockout | Treatment:1-O-Octadecyl-sn-glycerol | Sample.condition:Wholecell RAW_FILE_NAME=Golga5-Oct-WC-3_NonDerPos; RAW_FILE_NAME=Golga5-Oct-WC-3_NonDerNeg; RAW_FILE_NAME=Der_Pos 5in40_G5A_o3; RAW_FILE_NAME=LT01CB3T1-Sample__G5AO--B3_8X_MS_LP_230220_FAIMS_Neg_1 SUBJECT_SAMPLE_FACTORS - S129 GenoType:GOLGA5-knockout | Treatment:1-O-Octadecyl-sn-glycerol | Sample.condition:Wholecell RAW_FILE_NAME=Golga5-Oct-WC-4_NonDerPos; RAW_FILE_NAME=Golga5-Oct-WC-4_NonDerNeg; RAW_FILE_NAME=Der_Pos 5in40_G5A_o4; RAW_FILE_NAME=LT01CB4T1-Sample__G5AO--B4_8X_MS_LP_230220_FAIMS_Neg_1 SUBJECT_SAMPLE_FACTORS - S130 GenoType:GOLGA5-knockout | Treatment:1-O-Octadecyl-sn-glycerol | Sample.condition:Wholecell RAW_FILE_NAME=Golga5-Oct-WC-5_NonDerPos; RAW_FILE_NAME=Golga5-Oct-WC-5_NonDerNeg; RAW_FILE_NAME=Der_Pos 5in40_G5A_o5; RAW_FILE_NAME=LT01CB5T1-Sample__G5AO--B5_8X_MS_LP_230220_FAIMS_Neg_1 SUBJECT_SAMPLE_FACTORS - S131 GenoType:GOLGA5-knockout | Treatment:1-O-Hexadecyl-sn-glycerol | Sample.condition:Wholecell RAW_FILE_NAME=Golga5-Hex-WC-1_NonDerPos; RAW_FILE_NAME=Golga5-Hex-WC-1_NonDerNeg; RAW_FILE_NAME=Der_Pos 5in40_G5A_h1; RAW_FILE_NAME=LT01BB1T1-Sample__G5AH--B1_8X_MS_LP_230220_FAIMS_Neg_1 SUBJECT_SAMPLE_FACTORS - S132 GenoType:GOLGA5-knockout | Treatment:1-O-Hexadecyl-sn-glycerol | Sample.condition:Wholecell RAW_FILE_NAME=Golga5-Hex-WC-2_NonDerPos; RAW_FILE_NAME=Golga5-Hex-WC-2_NonDerNeg; RAW_FILE_NAME=Der_Pos 5in40_G5A_h2; RAW_FILE_NAME=LT01BB2T1-Sample__G5AH--B2_8X_MS_LP_230220_FAIMS_Neg_1 SUBJECT_SAMPLE_FACTORS - S133 GenoType:GOLGA5-knockout | Treatment:1-O-Hexadecyl-sn-glycerol | Sample.condition:Wholecell RAW_FILE_NAME=Golga5-Hex-WC-3_NonDerPos; RAW_FILE_NAME=Golga5-Hex-WC-3_NonDerNeg; RAW_FILE_NAME=Der_Pos 5in40_G5A_h3; RAW_FILE_NAME=LT01BB3T1-Sample__G5AH--B3_8X_MS_LP_230220_FAIMS_Neg_1 SUBJECT_SAMPLE_FACTORS - S134 GenoType:GOLGA5-knockout | Treatment:1-O-Hexadecyl-sn-glycerol | Sample.condition:Wholecell RAW_FILE_NAME=Golga5-Hex-WC-4_NonDerPos; RAW_FILE_NAME=Golga5-Hex-WC-4_NonDerNeg; RAW_FILE_NAME=Der_Pos 5in40_G5A_h4; RAW_FILE_NAME=LT01BB4T1-Sample__G5AH--B4_8X_MS_LP_230220_FAIMS_Neg_1 SUBJECT_SAMPLE_FACTORS - S135 GenoType:GOLGA5-knockout | Treatment:1-O-Hexadecyl-sn-glycerol | Sample.condition:Wholecell RAW_FILE_NAME=Golga5-Hex-WC-5_NonDerPos; RAW_FILE_NAME=Golga5-Hex-WC-5_NonDerNeg; RAW_FILE_NAME=Der_Pos 5in40_G5A_h5; RAW_FILE_NAME=LT01BB5T1-Sample__G5AH--B5_8X_MS_LP_230220_FAIMS_Neg_1 SUBJECT_SAMPLE_FACTORS - S136 GenoType:Pex26-knockout | Treatment:Vehicle | Sample.condition:wholecell RAW_FILE_NAME=Pex26-V-WC-1_NonDerPos; RAW_FILE_NAME=Pex26-V-WC-1_NonDerNeg; RAW_FILE_NAME=Der_Pos 5in40_P26_e1; RAW_FILE_NAME=LT01GB1T1-Sample__P26E--B1_8X_MS_LP_230220_FAIMS_Neg_1 SUBJECT_SAMPLE_FACTORS - S137 GenoType:Pex26-knockout | Treatment:Vehicle | Sample.condition:wholecell RAW_FILE_NAME=Pex26-V-WC-2_NonDerPos; RAW_FILE_NAME=Pex26-V-WC-2_NonDerNeg; RAW_FILE_NAME=Der_Pos 5in40_P26_e2; RAW_FILE_NAME=LT01GB2T1-Sample__P26E--B2_8X_MS_LP_230220_FAIMS_Neg_1 SUBJECT_SAMPLE_FACTORS - S138 GenoType:Pex26-knockout | Treatment:Vehicle | Sample.condition:wholecell RAW_FILE_NAME=Pex26-V-WC-3_NonDerPos; RAW_FILE_NAME=Pex26-V-WC-3_NonDerNeg; RAW_FILE_NAME=Der_Pos 5in40_P26_e3; RAW_FILE_NAME=LT01GB3T1-Sample__P26E--B3_8X_MS_LP_230220_FAIMS_Neg_1 SUBJECT_SAMPLE_FACTORS - S139 GenoType:Pex26-knockout | Treatment:Vehicle | Sample.condition:wholecell RAW_FILE_NAME=Pex26-V-WC-4_NonDerPos; RAW_FILE_NAME=Pex26-V-WC-4_NonDerNeg; RAW_FILE_NAME=Der_Pos 5in40_P26_e4; RAW_FILE_NAME=LT01GB4T1-Sample__P26E--B4_8X_MS_LP_230220_FAIMS_Neg_1 SUBJECT_SAMPLE_FACTORS - S140 GenoType:Pex26-knockout | Treatment:Vehicle | Sample.condition:wholecell RAW_FILE_NAME=Pex26-V-WC-5_NonDerPos; RAW_FILE_NAME=Pex26-V-WC-5_NonDerNeg; RAW_FILE_NAME=Der_Pos 5in40_P26_e5; RAW_FILE_NAME=LT01GB5T1-Sample__P26E--B5_8X_MS_LP_230220_FAIMS_Neg_1 SUBJECT_SAMPLE_FACTORS - S141 GenoType:Pex26-knockout | Treatment:1-O-Octadecyl-sn-glycerol | Sample.condition:Wholecell RAW_FILE_NAME=Pex26-Oct-WC-1_NonDerPos; RAW_FILE_NAME=Pex26-Oct-WC-1_NonDerNeg; RAW_FILE_NAME=Der_Pos 5in40_P26_o1; RAW_FILE_NAME=LT01IB1T1-Sample__P26O--B1_8X_MS_LP_230220_FAIMS_Neg_1 SUBJECT_SAMPLE_FACTORS - S142 GenoType:Pex26-knockout | Treatment:1-O-Octadecyl-sn-glycerol | Sample.condition:Wholecell RAW_FILE_NAME=Pex26-Oct-WC-2_NonDerPos; RAW_FILE_NAME=Pex26-Oct-WC-2_NonDerNeg; RAW_FILE_NAME=Der_Pos 5in40_P26_o2; RAW_FILE_NAME=LT01IB2T1-Sample__P26O--B2_8X_MS_LP_230220_FAIMS_Neg_1 SUBJECT_SAMPLE_FACTORS - S143 GenoType:Pex26-knockout | Treatment:1-O-Octadecyl-sn-glycerol | Sample.condition:Wholecell RAW_FILE_NAME=Pex26-Oct-WC-3_NonDerPos; RAW_FILE_NAME=Pex26-Oct-WC-3_NonDerNeg; RAW_FILE_NAME=Der_Pos 5in40_P26_o3; RAW_FILE_NAME=LT01IB3T1-Sample__P26O--B3_8X_MS_LP_230220_FAIMS_Neg_1 SUBJECT_SAMPLE_FACTORS - S144 GenoType:Pex26-knockout | Treatment:1-O-Octadecyl-sn-glycerol | Sample.condition:Wholecell RAW_FILE_NAME=Pex26-Oct-WC-4_NonDerPos; RAW_FILE_NAME=Pex26-Oct-WC-4_NonDerNeg; RAW_FILE_NAME=Der_Pos 5in40_P26_o4; RAW_FILE_NAME=LT01IB4T1-Sample__P26O--B4_8X_MS_LP_230220_FAIMS_Neg_1 SUBJECT_SAMPLE_FACTORS - S145 GenoType:Pex26-knockout | Treatment:1-O-Octadecyl-sn-glycerol | Sample.condition:Wholecell RAW_FILE_NAME=Pex26-Oct-WC-5_NonDerPos; RAW_FILE_NAME=Pex26-Oct-WC-5_NonDerNeg; RAW_FILE_NAME=Der_Pos 5in40_P26_o5; RAW_FILE_NAME=LT01IB5T1-Sample__P26O--B5_8X_MS_LP_230220_FAIMS_Neg_1 SUBJECT_SAMPLE_FACTORS - S146 GenoType:Pex26-knockout | Treatment:1-O-Hexadecyl-sn-glycerol | Sample.condition:Wholecell RAW_FILE_NAME=Pex26-Hex-WC-1_NonDerPos; RAW_FILE_NAME=Pex26-Hex-WC-1_NonDerNeg; RAW_FILE_NAME=Der_Pos 5in40_P26_h1; RAW_FILE_NAME=LT01HB1T1-Sample__P26H--B1_8X_MS_LP_230220_FAIMS_Neg_1 SUBJECT_SAMPLE_FACTORS - S147 GenoType:Pex26-knockout | Treatment:1-O-Hexadecyl-sn-glycerol | Sample.condition:Wholecell RAW_FILE_NAME=Pex26-Hex-WC-2_NonDerPos; RAW_FILE_NAME=Pex26-Hex-WC-2_NonDerNeg; RAW_FILE_NAME=Der_Pos 5in40_P26_h2; RAW_FILE_NAME=LT01HB2T1-Sample__P26H--B2_8X_MS_LP_230220_FAIMS_Neg_1 SUBJECT_SAMPLE_FACTORS - S148 GenoType:Pex26-knockout | Treatment:1-O-Hexadecyl-sn-glycerol | Sample.condition:Wholecell RAW_FILE_NAME=Pex26-Hex-WC-3_NonDerPos; RAW_FILE_NAME=Pex26-Hex-WC-3_NonDerNeg; RAW_FILE_NAME=Der_Pos 5in40_P26_h3; RAW_FILE_NAME=LT01HB3T1-Sample__P26H--B3_8X_MS_LP_230220_FAIMS_Neg_1 SUBJECT_SAMPLE_FACTORS - S149 GenoType:Pex26-knockout | Treatment:1-O-Hexadecyl-sn-glycerol | Sample.condition:Wholecell RAW_FILE_NAME=Pex26-Hex-WC-4_NonDerPos; RAW_FILE_NAME=Pex26-Hex-WC-4_NonDerNeg; RAW_FILE_NAME=Der_Pos 5in40_P26_h4; RAW_FILE_NAME=LT01HB4T1-Sample__P26H--B4_8X_MS_LP_230220_FAIMS_Neg_1 SUBJECT_SAMPLE_FACTORS - S150 GenoType:Pex26-knockout | Treatment:1-O-Hexadecyl-sn-glycerol | Sample.condition:Wholecell RAW_FILE_NAME=Pex26-Hex-WC-5_NonDerPos; RAW_FILE_NAME=Pex26-Hex-WC-5_NonDerNeg; RAW_FILE_NAME=Der_Pos 5in40_P26_h5; RAW_FILE_NAME=LT01HB5T1-Sample__P26H--B5_8X_MS_LP_230220_FAIMS_Neg_1 SUBJECT_SAMPLE_FACTORS - S151 GenoType:GOLGA5-knockout | Treatment:1-O-Hexadecyl-sn-glycerol | Sample.condition:Mitochondria RAW_FILE_NAME=230703_Golga5_H1_NonDerPos; RAW_FILE_NAME=230703_Golga5_H1_NonDerNeg; RAW_FILE_NAME=Golga5_h1_DerPos_5in40_method; RAW_FILE_NAME=GOGA5-h1-CV61 SUBJECT_SAMPLE_FACTORS - S152 GenoType:GOLGA5-knockout | Treatment:1-O-Hexadecyl-sn-glycerol | Sample.condition:Mitochondria RAW_FILE_NAME=230703_Golga5_H2_NonDerPos; RAW_FILE_NAME=230703_Golga5_H2_NonDerNeg; RAW_FILE_NAME=Golga5_h2_DerPos_5in40_method; RAW_FILE_NAME=GOGA5-h2-CV61 SUBJECT_SAMPLE_FACTORS - S153 GenoType:GOLGA5-knockout | Treatment:1-O-Hexadecyl-sn-glycerol | Sample.condition:Mitochondria RAW_FILE_NAME=230703_Golga5_H3_NonDerPos; RAW_FILE_NAME=230703_Golga5_H3_NonDerNeg; RAW_FILE_NAME=Golga5_h3_DerPos_5in40_method_20230710040701; RAW_FILE_NAME=GOGA5-h3-CV61 SUBJECT_SAMPLE_FACTORS - S154 GenoType:GOLGA5-knockout | Treatment:Vehicle | Sample.condition:Mitochondria RAW_FILE_NAME=230703_Golga5_E1_NonDerPos; RAW_FILE_NAME=230703_Golga5_E1_NonDerNeg; RAW_FILE_NAME=Golga5_e1_DerPos_5in40_method; RAW_FILE_NAME=GOGA5-E1-CV61 SUBJECT_SAMPLE_FACTORS - S155 GenoType:GOLGA5-knockout | Treatment:Vehicle | Sample.condition:Mitochondria RAW_FILE_NAME=230703_Golga5_E2_NonDerPos; RAW_FILE_NAME=230703_Golga5_E2_NonDerNeg; RAW_FILE_NAME=Golga5_e2_DerPos_5in40_method; RAW_FILE_NAME=GOGA5-E2-CV61 SUBJECT_SAMPLE_FACTORS - S156 GenoType:GOLGA5-knockout | Treatment:Vehicle | Sample.condition:Mitochondria RAW_FILE_NAME=230703_Golga5_E3_NonDerPos; RAW_FILE_NAME=230703_Golga5_E3_NonDerNeg; RAW_FILE_NAME=Golga5_e3_DerPos_5in40_method; RAW_FILE_NAME=GOGA5-E3-CV61 SUBJECT_SAMPLE_FACTORS - S157 GenoType:GOLGA8M-knockout | Treatment:1-O-Hexadecyl-sn-glycerol | Sample.condition:Mitochondria RAW_FILE_NAME=230703_Golga8M_H1_NonDerPos; RAW_FILE_NAME=230703_Golga8M_H1_NonDerNeg; RAW_FILE_NAME=Golga8m_h1_DerPos_5in40_method_20230710043207; RAW_FILE_NAME=GOGA8-h1-CV61 SUBJECT_SAMPLE_FACTORS - S158 GenoType:GOLGA8M-knockout | Treatment:1-O-Hexadecyl-sn-glycerol | Sample.condition:Mitochondria RAW_FILE_NAME=230703_Golga8M_H2_NonDerPos; RAW_FILE_NAME=230703_Golga8M_H2_NonDerNeg; RAW_FILE_NAME=Golga8m_h2_DerPos_5in40_method; RAW_FILE_NAME=GOGA8-h2-CV61 SUBJECT_SAMPLE_FACTORS - S159 GenoType:GOLGA8M-knockout | Treatment:1-O-Hexadecyl-sn-glycerol | Sample.condition:Mitochondria RAW_FILE_NAME=230703_Golga8M_H3_NonDerPos; RAW_FILE_NAME=230703_Golga8M_H3_NonDerNeg; RAW_FILE_NAME=Golga8m_h3_DerPos_5in40_method; RAW_FILE_NAME=GOGA8-h3-CV61 SUBJECT_SAMPLE_FACTORS - S160 GenoType:GOLGA8M-knockout | Treatment:Vehicle | Sample.condition:Mitochondria RAW_FILE_NAME=230703_Golga8M_E1_NonDerPos; RAW_FILE_NAME=230703_Golga8M_E1_NonDerNeg; RAW_FILE_NAME=Golga8m_e1_DerPos_5in40_method_20230710035354; RAW_FILE_NAME=GOGA8-e1-CV61 SUBJECT_SAMPLE_FACTORS - S161 GenoType:GOLGA8M-knockout | Treatment:Vehicle | Sample.condition:Mitochondria RAW_FILE_NAME=230703_Golga8M_E2_NonDerPos; RAW_FILE_NAME=230703_Golga8M_E2_NonDerNeg; RAW_FILE_NAME=Golga8m_e2_DerPos_5in40_method_20230710041449; RAW_FILE_NAME=GOGA8-e2-CV61 SUBJECT_SAMPLE_FACTORS - S162 GenoType:GOLGA8M-knockout | Treatment:Vehicle | Sample.condition:Mitochondria RAW_FILE_NAME=230703_Golga8M_E3_NonDerPos; RAW_FILE_NAME=230703_Golga8M_E3_NonDerNeg; RAW_FILE_NAME=Golga8m_e3_DerPos_5in40_method; RAW_FILE_NAME=GOGA8-e3-CV61 SUBJECT_SAMPLE_FACTORS - S163 GenoType:Pex26-knockout | Treatment:1-O-Hexadecyl-sn-glycerol | Sample.condition:Mitochondria RAW_FILE_NAME=230703_Pex26-H1_NonDerPos; RAW_FILE_NAME=230703_Pex26-H1_NonDerNeg; RAW_FILE_NAME=PEX_H1_DerPos_5in40_method; RAW_FILE_NAME=Pex26-H1-CV61 SUBJECT_SAMPLE_FACTORS - S164 GenoType:Pex26-knockout | Treatment:1-O-Hexadecyl-sn-glycerol | Sample.condition:Mitochondria RAW_FILE_NAME=230703_Pex26-H2_NonDerPos; RAW_FILE_NAME=230703_Pex26-H2_NonDerNeg; RAW_FILE_NAME=PEX_H2_DerPos_5in40_method; RAW_FILE_NAME=Pex26-H2-CV61 SUBJECT_SAMPLE_FACTORS - S165 GenoType:Pex26-knockout | Treatment:1-O-Hexadecyl-sn-glycerol | Sample.condition:Mitochondria RAW_FILE_NAME=230703_Pex26-H3_NonDerPos; RAW_FILE_NAME=230703_Pex26-H3_NonDerNeg; RAW_FILE_NAME=PEX_H3_DerPos_5in40_method; RAW_FILE_NAME=Pex26-H3-CV61 SUBJECT_SAMPLE_FACTORS - S166 GenoType:Pex26-knockout | Treatment:Vehicle | Sample.condition:Mitochondria RAW_FILE_NAME=230703_Pex26-E1_NonDerPos; RAW_FILE_NAME=230703_Pex26-E1_NonDerNeg; RAW_FILE_NAME=PEX_E1_DerPos_5in40_method; RAW_FILE_NAME=Pex26-E1-CV61 SUBJECT_SAMPLE_FACTORS - S167 GenoType:Pex26-knockout | Treatment:Vehicle | Sample.condition:Mitochondria RAW_FILE_NAME=230703_Pex26-E2_NonDerPos; RAW_FILE_NAME=230703_Pex26-E2_NonDerNeg; RAW_FILE_NAME=PEX_E2_DerPos_5in40_method; RAW_FILE_NAME=Pex26-E2-CV61 SUBJECT_SAMPLE_FACTORS - S168 GenoType:Pex26-knockout | Treatment:Vehicle | Sample.condition:Mitochondria RAW_FILE_NAME=230703_Pex26-E3_NonDerPos; RAW_FILE_NAME=230703_Pex26-E3_NonDerNeg; RAW_FILE_NAME=PEX_E3_DerPos_5in40_method; RAW_FILE_NAME=Pex26-E3-CV61 SUBJECT_SAMPLE_FACTORS - S169 GenoType:SEC61A2-knockout | Treatment:1-O-Hexadecyl-sn-glycerol | Sample.condition:Mitochondria RAW_FILE_NAME=230703_SEC61_H1_NonDerPos; RAW_FILE_NAME=230703_SEC61_H1_NonDerNeg; RAW_FILE_NAME=SEC61_H1_DerPos_5in40_method; RAW_FILE_NAME=SEC61-H1-CV61 SUBJECT_SAMPLE_FACTORS - S170 GenoType:SEC61A2-knockout | Treatment:1-O-Hexadecyl-sn-glycerol | Sample.condition:Mitochondria RAW_FILE_NAME=230703_SEC61_H2_NonDerPos; RAW_FILE_NAME=230703_SEC61_H2_NonDerNeg; RAW_FILE_NAME=SEC61_H2_DerPos_5in40_method; RAW_FILE_NAME=SEC61-H2-CV61 SUBJECT_SAMPLE_FACTORS - S171 GenoType:SEC61A2-knockout | Treatment:1-O-Hexadecyl-sn-glycerol | Sample.condition:Mitochondria RAW_FILE_NAME=230703_SEC61_H3_NonDerPos; RAW_FILE_NAME=230703_SEC61_H3_NonDerNeg; RAW_FILE_NAME=SEC61_H3_DerPos_5in40_method; RAW_FILE_NAME=SEC61-H3-CV61 SUBJECT_SAMPLE_FACTORS - S172 GenoType:SEC61A2-knockout | Treatment:Vehicle | Sample.condition:Mitochondria RAW_FILE_NAME=230703_SEC61_E1_NonDerPos; RAW_FILE_NAME=230703_SEC61_E1_NonDerNeg; RAW_FILE_NAME=SEC61_E1_DerPos_5in40_method; RAW_FILE_NAME=SEC61-E1-CV61 SUBJECT_SAMPLE_FACTORS - S173 GenoType:SEC61A2-knockout | Treatment:Vehicle | Sample.condition:Mitochondria RAW_FILE_NAME=230703_SEC61_E2_NonDerPos; RAW_FILE_NAME=230703_SEC61_E2_NonDerNeg; RAW_FILE_NAME=SEC61_E2_DerPos_5in40_method; RAW_FILE_NAME=SEC61-E2-CV61 SUBJECT_SAMPLE_FACTORS - S174 GenoType:SEC61A2-knockout | Treatment:Vehicle | Sample.condition:Mitochondria RAW_FILE_NAME=230703_SEC61_E3_NonDerPos; RAW_FILE_NAME=230703_SEC61_E3_NonDerNeg; RAW_FILE_NAME=SEC61_E3_DerPos_5in40_method; RAW_FILE_NAME=SEC61-E3-CV61 SUBJECT_SAMPLE_FACTORS - S175 GenoType:SEC62-knockout | Treatment:1-O-Hexadecyl-sn-glycerol | Sample.condition:Mitochondria RAW_FILE_NAME=230703_SEC62_H1_NonDerPos; RAW_FILE_NAME=230703_SEC62_H1_NonDerNeg; RAW_FILE_NAME=SEC62_H1_DerPos_5in40_method; RAW_FILE_NAME=SEC62-H1-CV61 SUBJECT_SAMPLE_FACTORS - S176 GenoType:SEC62-knockout | Treatment:1-O-Hexadecyl-sn-glycerol | Sample.condition:Mitochondria RAW_FILE_NAME=230703_SEC62_H2_NonDerPos; RAW_FILE_NAME=230703_SEC62_H2_NonDerNeg; RAW_FILE_NAME=SEC62_H2_DerPos_5in40_method; RAW_FILE_NAME=SEC62-H2-CV61 SUBJECT_SAMPLE_FACTORS - S177 GenoType:SEC62-knockout | Treatment:1-O-Hexadecyl-sn-glycerol | Sample.condition:Mitochondria RAW_FILE_NAME=230703_SEC62_H3_NonDerPos; RAW_FILE_NAME=230703_SEC62_H3_NonDerNeg; RAW_FILE_NAME=SEC62_H3_DerPos_5in40_method; RAW_FILE_NAME=SEC62-h3-CV61_20230704044638 SUBJECT_SAMPLE_FACTORS - S178 GenoType:SEC62-knockout | Treatment:Vehicle | Sample.condition:Mitochondria RAW_FILE_NAME=230703_SEC62_E1_NonDerPos; RAW_FILE_NAME=230703_SEC62_E1_NonDerNeg; RAW_FILE_NAME=SEC62_E1_DerPos_5in40_method; RAW_FILE_NAME=SEC62-E1-CV61 SUBJECT_SAMPLE_FACTORS - S179 GenoType:SEC62-knockout | Treatment:Vehicle | Sample.condition:Mitochondria RAW_FILE_NAME=230703_SEC62_E2_NonDerPos; RAW_FILE_NAME=230703_SEC62_E2_NonDerNeg; RAW_FILE_NAME=SEC62_E2_DerPos_5in40_method; RAW_FILE_NAME=SEC62-E2-CV61 SUBJECT_SAMPLE_FACTORS - S180 GenoType:SEC62-knockout | Treatment:Vehicle | Sample.condition:Mitochondria RAW_FILE_NAME=230703_SEC62_E3_NonDerPos; RAW_FILE_NAME=230703_SEC62_E3_NonDerNeg; RAW_FILE_NAME=SEC62_E3_DerPos_5in40_method; RAW_FILE_NAME=SEC62-E3-CV61_20230704044254 SUBJECT_SAMPLE_FACTORS - S181 GenoType:Wild-type | Treatment:1-O-Hexadecyl-sn-glycerol | Sample.condition:Mitochondria RAW_FILE_NAME=230703_WT-H1_NonDerPos; RAW_FILE_NAME=230703_WT-H1_NonDerNeg; RAW_FILE_NAME=WT_h1_DerPos_5in40_method; RAW_FILE_NAME=WT-H1-CV61 SUBJECT_SAMPLE_FACTORS - S182 GenoType:Wild-type | Treatment:1-O-Hexadecyl-sn-glycerol | Sample.condition:Mitochondria RAW_FILE_NAME=230703_WT-H2_NonDerPos; RAW_FILE_NAME=230703_WT-H2_NonDerNeg; RAW_FILE_NAME=WT_h2_DerPos_5in40_method; RAW_FILE_NAME=WT-H2-CV61 SUBJECT_SAMPLE_FACTORS - S183 GenoType:Wild-type | Treatment:1-O-Hexadecyl-sn-glycerol | Sample.condition:Mitochondria RAW_FILE_NAME=230703_WT-H3_NonDerPos; RAW_FILE_NAME=230703_WT-H3_NonDerNeg; RAW_FILE_NAME=WT_h3_DerPos_5in40_method; RAW_FILE_NAME=WT-H3-CV61 SUBJECT_SAMPLE_FACTORS - S184 GenoType:Wild-type | Treatment:Vehicle | Sample.condition:Mitochondria RAW_FILE_NAME=230703_WT-E1_NonDerPos; RAW_FILE_NAME=230703_WT-E1_NonDerNeg; RAW_FILE_NAME=WT_E1_DerPos_5in40_method; RAW_FILE_NAME=WT-E1-CV61 SUBJECT_SAMPLE_FACTORS - S185 GenoType:Wild-type | Treatment:Vehicle | Sample.condition:Mitochondria RAW_FILE_NAME=230703_WT-E2_NonDerPos; RAW_FILE_NAME=230703_WT-E2_NonDerNeg; RAW_FILE_NAME=WT_E2_DerPos_5in40_method; RAW_FILE_NAME=WT-E2-CV61 SUBJECT_SAMPLE_FACTORS - S186 GenoType:Wild-type | Treatment:Vehicle | Sample.condition:Mitochondria RAW_FILE_NAME=230703_WT-E3_NonDerPos; RAW_FILE_NAME=230703_WT-E3_NonDerNeg; RAW_FILE_NAME=WT_E3_DerPos_5in40_method; RAW_FILE_NAME=WT-E3-CV61 #COLLECTION CO:COLLECTION_SUMMARY Whole Cell pellets were counted, washed with PBS 3 times, and freeze-dried. CO:COLLECTION_SUMMARY Mitochondria were isolated from CAL51 cells by differential centrifugation in a CO:COLLECTION_SUMMARY buffer containing 250 mM sucrose, 1 mM EGTA, and 5 mM Tris-HCl (pH 7.4). The CO:COLLECTION_SUMMARY protein amount was determined with BCA assay CO:SAMPLE_TYPE Cultured cells #TREATMENT TR:TREATMENT_SUMMARY The peroxisomal import protein PEX26, Golgi organisation protein GOLGA5, a Golgi TR:TREATMENT_SUMMARY protein GOLGA8M, an ER protein SEC61A2, ER protein import regulator and stress TR:TREATMENT_SUMMARY responder SEC62 were each knocked out in CAL51 breast cells using CRISPR/Cas9 TR:TREATMENT_SUMMARY gene editing. The lipid profile of each knockout cell line and control cells was TR:TREATMENT_SUMMARY analysed as well as the lipid profile of control and five knockout cell lines TR:TREATMENT_SUMMARY treated with either vehicle (ethanol), 20 µM 1-O-Hexadecyl-sn-glycerol TR:TREATMENT_SUMMARY (16:O-AG), or 20 µM 1-O-Octadecyl-sn-glycerol (18:O-AG) for 24 h. #SAMPLEPREP SP:SAMPLEPREP_SUMMARY samples pellets were homogenized with a bullet blender. Lipids were extracted SP:SAMPLEPREP_SUMMARY with house-made lipid internal standard mix in Methanol: Choloform: SP:SAMPLEPREP_SUMMARY water(v/v/v,2/1/0.74). Extracts were dried down with speed vac, then resuspended SP:SAMPLEPREP_SUMMARY in IPA:MEOH: CHCL3(v/v/v,4/2/1) and stored at -80℃ before measurement. SP:SAMPLEPREP_SUMMARY Nonderivatized samples were reconstituted in IPA:MEOH: CHCL3(v/v/v,4/2/1)+20mM SP:SAMPLEPREP_SUMMARY Ammonium Formate and injected into Thermo LUMOS with Advion Nanomate. SP:SAMPLEPREP_SUMMARY Aminophospholipids were derivatized with 13C1−S, SP:SAMPLEPREP_SUMMARY S′-dimethylthiobutanoyl-N-hydroxysuccinimide ester (13C1−DMBNHS) and SP:SAMPLEPREP_SUMMARY plasmalogen was derivatized with iodine and methanol, and reconstituted in SP:SAMPLEPREP_SUMMARY IPA:MEOH: CHCL3(v/v/v,4/2/1)+20mMAF for MS measurement. Extracts were dilute SP:SAMPLEPREP_SUMMARY with IPA:MEOH: CHCL3(v/v/v,4/2/1) before FAIMS-MS measurement. SP:EXTRACTION_METHOD monophase lipid extraction SP:EXTRACT_STORAGE -80℃ SP:SAMPLE_DERIVATIZATION 13C1−DMBNHS, Iodine&Methanol #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE None (Direct infusion) CH:INSTRUMENT_NAME none CH:COLUMN_NAME none CH:SOLVENT_A None CH:SOLVENT_B None CH:FLOW_GRADIENT None CH:FLOW_RATE None CH:COLUMN_TEMPERATURE None #ANALYSIS AN:ANALYSIS_TYPE MS #MS MS:INSTRUMENT_NAME Thermo Orbitrap Fusion Lumos Tribrid MS:INSTRUMENT_TYPE Orbitrap MS:MS_TYPE ESI MS:ION_MODE POSITIVE MS:MS_COMMENTS Samples were introduced to an Orbitrap Fusion Lumos ultra-high resolution/ MS:MS_COMMENTS accurate mass spectrometer (Thermo Fisher Scientific) using an Advion Triversa MS:MS_COMMENTS Nanomate nano electrospray ionization (nESI) source (Advion). The nESI gas MS:MS_COMMENTS pressure was set to 0.3 psi and the spray voltage to 1.1 kV. The Orbitrap Fusion MS:MS_COMMENTS Lumos ion transfer capillary temperature was set to 150˚C, the RF-value to 10%, MS:MS_COMMENTS the AGC target to 2x105 and mass resolving power at 500,000 (at 200 m/z). All MS:MS_COMMENTS spectra were recorded in positive ionization mode for a period of 3 minutes over MS:MS_COMMENTS a mass range of 350-1600 m/z. MS:MS_RESULTS_FILE ST002838_AN004662_Results.txt UNITS:PeakArea Has m/z:Yes Has RT:No RT units:No RT data #END