#METABOLOMICS WORKBENCH syfoods_20240217_165608 DATATRACK_ID:4652 STUDY_ID:ST003094 ANALYSIS_ID:AN005064 PROJECT_ID:PR001923
VERSION                          	1
CREATED_ON                       	07-05-2024
#PROJECT
PR:PROJECT_TITLE                 	Lactiplantibacillus plantarum intervention on gestational health of gestational
PR:PROJECT_TITLE                 	fecal microbiome transplant germ-free mice
PR:PROJECT_SUMMARY               	The study aims to investigate whether administration of probiotic L. plantarum
PR:PROJECT_SUMMARY               	could influence comprehensive gestational health by using germ-free mice, and
PR:PROJECT_SUMMARY               	characterizing its influence on relationships between the gut microbiome
PR:PROJECT_SUMMARY               	composition and metabolism.
PR:INSTITUTE                     	Beijing Sanyuan Foods Co. Ltd
PR:LAST_NAME                     	Lijun
PR:FIRST_NAME                    	Chen
PR:ADDRESS                       	8th yingchang street, yinghai distric
PR:EMAIL                         	chenlijun@sanyuan.com.cn
PR:PHONE                         	01056306594
PR:DOI                           	http://dx.doi.org/10.21228/M85149
#STUDY
ST:STUDY_TITLE                   	Lactiplantibacillus plantarum intervention on serum untargeted metabolome of
ST:STUDY_TITLE                   	gestational fecal microbiome transplant germ-free mice
ST:STUDY_SUMMARY                 	The study aims to investigate whether administration of probiotic L. plantarum
ST:STUDY_SUMMARY                 	could influence profiling of serum untargeted metabolome of germ-free mice.
ST:STUDY_SUMMARY                 	Results showed that there were 23 metabolites significantly different in
ST:STUDY_SUMMARY                 	intervention group.
ST:INSTITUTE                     	Beijing Sanyuan Foods Co. Ltd
ST:LAST_NAME                     	Lijun
ST:FIRST_NAME                    	Chen
ST:ADDRESS                       	8th yingchang street, yinghai distric
ST:EMAIL                         	chenlijun@sanyuan.com.cn
ST:PHONE                         	01056306594
ST:SUBMIT_DATE                   	2024-02-17
#SUBJECT
SU:SUBJECT_TYPE                  	Mammal
SU:SUBJECT_SPECIES               	Mus musculus
SU:TAXONOMY_ID                   	10090
SU:GENDER                        	Female
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data
SUBJECT_SAMPLE_FACTORS           	-	185	Sample source:serum | Factor:control	RAW_FILE_NAME=185.raw
SUBJECT_SAMPLE_FACTORS           	-	188	Sample source:serum | Factor:control	RAW_FILE_NAME=188.raw
SUBJECT_SAMPLE_FACTORS           	-	908	Sample source:serum | Factor:control	RAW_FILE_NAME=908.raw
SUBJECT_SAMPLE_FACTORS           	-	909	Sample source:serum | Factor:control	RAW_FILE_NAME=909.raw
SUBJECT_SAMPLE_FACTORS           	-	107	Sample source:serum | Factor:intervention	RAW_FILE_NAME=107.raw
SUBJECT_SAMPLE_FACTORS           	-	120	Sample source:serum | Factor:intervention	RAW_FILE_NAME=120.raw
SUBJECT_SAMPLE_FACTORS           	-	122	Sample source:serum | Factor:intervention	RAW_FILE_NAME=122.raw
SUBJECT_SAMPLE_FACTORS           	-	180	Sample source:serum | Factor:intervention	RAW_FILE_NAME=180.raw
SUBJECT_SAMPLE_FACTORS           	-	81	Sample source:serum | Factor:intervention	RAW_FILE_NAME=81.raw
SUBJECT_SAMPLE_FACTORS           	-	907	Sample source:serum | Factor:intervention	RAW_FILE_NAME=907.raw
#COLLECTION
CO:COLLECTION_SUMMARY            	Blood was collected and centrifuged at 3,000 rpm for 10 min and stored at -80°C
CO:COLLECTION_SUMMARY            	environment
CO:SAMPLE_TYPE                   	Blood (serum)
CO:STORAGE_CONDITIONS            	-80℃
#TREATMENT
TR:TREATMENT_SUMMARY             	mice were randomly assigned into two experimental groups, named control and
TR:TREATMENT_SUMMARY             	intervention groups, receiving standard chow feeding, and standard chow + L.
TR:TREATMENT_SUMMARY             	plantarum. Probiotic was fed at a dose of 1*10^9 CFU/ml/kg body weight in PBS
TR:TREATMENT_SUMMARY             	solution by oral gavage (2 days/week, p.o., three weeks).
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	Samples were vortex for 1 min after adding 0.4 mL methanol; then they were
SP:SAMPLEPREP_SUMMARY            	centrifuged for 10 min at 12,000 rpm at 4^oC, and the supernatant was dried and
SP:SAMPLEPREP_SUMMARY            	resolved using 0.15 mL 2-chloro-l-phenylalanine (4 ppm) solution, and then
SP:SAMPLEPREP_SUMMARY            	filtered by 0.22 μm membrane.
SP:PROCESSING_STORAGE_CONDITIONS 	4℃
SP:EXTRACT_STORAGE               	-80℃
#CHROMATOGRAPHY
CH:INSTRUMENT_NAME               	Thermo Vanquish
CH:COLUMN_NAME                   	Waters ACQUITY UPLC HSS T3 (150 x 2.1mm,1.8um)
CH:COLUMN_TEMPERATURE            	40
CH:FLOW_GRADIENT                 	0-1 min, 2% B; 1-9 min, 2%-50% B; 9-12 min, 50%-98% B; 12-13.5 min, 98% B;
CH:FLOW_GRADIENT                 	13.5-14 min, 98%-2% B; 14-20 min, 2% B
CH:FLOW_RATE                     	0.25 mL/min
CH:SOLVENT_A                     	100% Water; 0.1% Formic acid
CH:SOLVENT_B                     	100% Acetonitrile; 0.1% Formic acid
CH:CHROMATOGRAPHY_TYPE           	LC
#ANALYSIS
AN:ANALYSIS_TYPE                 	MS
#MS
MS:INSTRUMENT_NAME               	Thermo Orbitrap Exploris 120
MS:INSTRUMENT_TYPE               	Orbitrap
MS:MS_TYPE                       	ESI
MS:MS_COMMENTS                   	MS1 range was 100-1000 m/z, and the number of data-dependent scans per cycle was
MS:MS_COMMENTS                   	4; MS/MS resolving power was 15000 FWHM, normalized collision energy was 30%,
MS:MS_COMMENTS                   	and dynamic exclusion time was automatic. The raw data were firstly converted to
MS:MS_COMMENTS                   	mzXML format by MSConvert in the ProteoWizard software package (v3.0.8789)
MS:MS_COMMENTS                   	(Adusumilli et al., 2017) and processed using XCMS (Domingo-Almenara et al.,
MS:MS_COMMENTS                   	2020) for feature detection, retention time correction and alignment.
MS:ION_MODE                      	POSITIVE
MS:MS_RESULTS_FILE               	ST003094_AN005064_Results.txt	UNITS:m/z values	Has m/z:Yes	Has RT:Yes	RT units:Minutes
#END