#METABOLOMICS WORKBENCH syfoods_20240217_165608 DATATRACK_ID:4652 STUDY_ID:ST003094 ANALYSIS_ID:AN005064 PROJECT_ID:PR001923 VERSION 1 CREATED_ON 07-05-2024 #PROJECT PR:PROJECT_TITLE Lactiplantibacillus plantarum intervention on gestational health of gestational PR:PROJECT_TITLE fecal microbiome transplant germ-free mice PR:PROJECT_SUMMARY The study aims to investigate whether administration of probiotic L. plantarum PR:PROJECT_SUMMARY could influence comprehensive gestational health by using germ-free mice, and PR:PROJECT_SUMMARY characterizing its influence on relationships between the gut microbiome PR:PROJECT_SUMMARY composition and metabolism. PR:INSTITUTE Beijing Sanyuan Foods Co. Ltd PR:LAST_NAME Lijun PR:FIRST_NAME Chen PR:ADDRESS 8th yingchang street, yinghai distric PR:EMAIL chenlijun@sanyuan.com.cn PR:PHONE 01056306594 PR:DOI http://dx.doi.org/10.21228/M85149 #STUDY ST:STUDY_TITLE Lactiplantibacillus plantarum intervention on serum untargeted metabolome of ST:STUDY_TITLE gestational fecal microbiome transplant germ-free mice ST:STUDY_SUMMARY The study aims to investigate whether administration of probiotic L. plantarum ST:STUDY_SUMMARY could influence profiling of serum untargeted metabolome of germ-free mice. ST:STUDY_SUMMARY Results showed that there were 23 metabolites significantly different in ST:STUDY_SUMMARY intervention group. ST:INSTITUTE Beijing Sanyuan Foods Co. Ltd ST:LAST_NAME Lijun ST:FIRST_NAME Chen ST:ADDRESS 8th yingchang street, yinghai distric ST:EMAIL chenlijun@sanyuan.com.cn ST:PHONE 01056306594 ST:SUBMIT_DATE 2024-02-17 #SUBJECT SU:SUBJECT_TYPE Mammal SU:SUBJECT_SPECIES Mus musculus SU:TAXONOMY_ID 10090 SU:GENDER Female #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS - 185 Sample source:serum | Factor:control RAW_FILE_NAME=185.raw SUBJECT_SAMPLE_FACTORS - 188 Sample source:serum | Factor:control RAW_FILE_NAME=188.raw SUBJECT_SAMPLE_FACTORS - 908 Sample source:serum | Factor:control RAW_FILE_NAME=908.raw SUBJECT_SAMPLE_FACTORS - 909 Sample source:serum | Factor:control RAW_FILE_NAME=909.raw SUBJECT_SAMPLE_FACTORS - 107 Sample source:serum | Factor:intervention RAW_FILE_NAME=107.raw SUBJECT_SAMPLE_FACTORS - 120 Sample source:serum | Factor:intervention RAW_FILE_NAME=120.raw SUBJECT_SAMPLE_FACTORS - 122 Sample source:serum | Factor:intervention RAW_FILE_NAME=122.raw SUBJECT_SAMPLE_FACTORS - 180 Sample source:serum | Factor:intervention RAW_FILE_NAME=180.raw SUBJECT_SAMPLE_FACTORS - 81 Sample source:serum | Factor:intervention RAW_FILE_NAME=81.raw SUBJECT_SAMPLE_FACTORS - 907 Sample source:serum | Factor:intervention RAW_FILE_NAME=907.raw #COLLECTION CO:COLLECTION_SUMMARY Blood was collected and centrifuged at 3,000 rpm for 10 min and stored at -80°C CO:COLLECTION_SUMMARY environment CO:SAMPLE_TYPE Blood (serum) CO:STORAGE_CONDITIONS -80℃ #TREATMENT TR:TREATMENT_SUMMARY mice were randomly assigned into two experimental groups, named control and TR:TREATMENT_SUMMARY intervention groups, receiving standard chow feeding, and standard chow + L. TR:TREATMENT_SUMMARY plantarum. Probiotic was fed at a dose of 1*10^9 CFU/ml/kg body weight in PBS TR:TREATMENT_SUMMARY solution by oral gavage (2 days/week, p.o., three weeks). #SAMPLEPREP SP:SAMPLEPREP_SUMMARY Samples were vortex for 1 min after adding 0.4 mL methanol; then they were SP:SAMPLEPREP_SUMMARY centrifuged for 10 min at 12,000 rpm at 4^oC, and the supernatant was dried and SP:SAMPLEPREP_SUMMARY resolved using 0.15 mL 2-chloro-l-phenylalanine (4 ppm) solution, and then SP:SAMPLEPREP_SUMMARY filtered by 0.22 μm membrane. SP:PROCESSING_STORAGE_CONDITIONS 4℃ SP:EXTRACT_STORAGE -80℃ #CHROMATOGRAPHY CH:INSTRUMENT_NAME Thermo Vanquish CH:COLUMN_NAME Waters ACQUITY UPLC HSS T3 (150 x 2.1mm,1.8um) CH:COLUMN_TEMPERATURE 40 CH:FLOW_GRADIENT 0-1 min, 2% B; 1-9 min, 2%-50% B; 9-12 min, 50%-98% B; 12-13.5 min, 98% B; CH:FLOW_GRADIENT 13.5-14 min, 98%-2% B; 14-20 min, 2% B CH:FLOW_RATE 0.25 mL/min CH:SOLVENT_A 100% Water; 0.1% Formic acid CH:SOLVENT_B 100% Acetonitrile; 0.1% Formic acid CH:CHROMATOGRAPHY_TYPE LC #ANALYSIS AN:ANALYSIS_TYPE MS #MS MS:INSTRUMENT_NAME Thermo Orbitrap Exploris 120 MS:INSTRUMENT_TYPE Orbitrap MS:MS_TYPE ESI MS:MS_COMMENTS MS1 range was 100-1000 m/z, and the number of data-dependent scans per cycle was MS:MS_COMMENTS 4; MS/MS resolving power was 15000 FWHM, normalized collision energy was 30%, MS:MS_COMMENTS and dynamic exclusion time was automatic. The raw data were firstly converted to MS:MS_COMMENTS mzXML format by MSConvert in the ProteoWizard software package (v3.0.8789) MS:MS_COMMENTS (Adusumilli et al., 2017) and processed using XCMS (Domingo-Almenara et al., MS:MS_COMMENTS 2020) for feature detection, retention time correction and alignment. MS:ION_MODE POSITIVE MS:MS_RESULTS_FILE ST003094_AN005064_Results.txt UNITS:m/z values Has m/z:Yes Has RT:Yes RT units:Minutes #END