#METABOLOMICS WORKBENCH syfoods_20240218_173637 DATATRACK_ID:4656 STUDY_ID:ST003098 ANALYSIS_ID:AN005069 PROJECT_ID:PR001923 VERSION 1 CREATED_ON 07-06-2024 #PROJECT PR:PROJECT_TITLE Lactiplantibacillus plantarum intervention on gestational health of gestational PR:PROJECT_TITLE fecal microbiome transplant germ-free mice PR:PROJECT_SUMMARY The study aims to investigate whether administration of probiotic L. plantarum PR:PROJECT_SUMMARY could influence comprehensive gestational health by using germ-free mice, and PR:PROJECT_SUMMARY characterizing its influence on relationships between the gut microbiome PR:PROJECT_SUMMARY composition and metabolism. PR:INSTITUTE Beijing Sanyuan Foods Co. Ltd PR:LAST_NAME Lijun PR:FIRST_NAME Chen PR:ADDRESS 8th yingchang street, yinghai distric PR:EMAIL chenlijun@sanyuan.com.cn PR:PHONE 01056306594 PR:DOI http://dx.doi.org/10.21228/M85149 #STUDY ST:STUDY_TITLE Lactiplantibacillus plantarum intervention on cecum tryptophans of gestational ST:STUDY_TITLE fecal microbiome transplant germ-free mice ST:STUDY_SUMMARY The study aims to investigate whether administration of probiotic L. plantarum ST:STUDY_SUMMARY could influence profiling of cecum tryptophans of germ-free mice. L. plantarum ST:STUDY_SUMMARY intervention during gestation of humanized GF mice showed no significant ST:STUDY_SUMMARY difference on tryptophans’s content. ST:INSTITUTE Beijing Sanyuan Foods Co. Ltd ST:LAST_NAME Lijun ST:FIRST_NAME Chen ST:ADDRESS 8th yingchang street, yinghai district ST:EMAIL chenlijun@sanyuan.com.cn ST:PHONE 01056306594 ST:SUBMIT_DATE 2024-02-18 #SUBJECT SU:SUBJECT_TYPE Mammal SU:SUBJECT_SPECIES Mus musculus SU:TAXONOMY_ID 10090 SU:GENDER Female #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS - 185MN Sample source:cecum content | Factor:control RAW_FILE_NAME=Wangyaru-P220801105.mzML SUBJECT_SAMPLE_FACTORS - 188MN Sample source:cecum content | Factor:control RAW_FILE_NAME=Wangyaru-P220801103.mzML SUBJECT_SAMPLE_FACTORS - 908MN Sample source:cecum content | Factor:control RAW_FILE_NAME=Wangyaru-P220801102.mzML SUBJECT_SAMPLE_FACTORS - 909MN Sample source:cecum content | Factor:control RAW_FILE_NAME=Wangyaru-P220801104.mzML SUBJECT_SAMPLE_FACTORS - 107MN Sample source:cecum content | Factor:intervention RAW_FILE_NAME=Wangyaru-P220801109.mzML SUBJECT_SAMPLE_FACTORS - 120MN Sample source:cecum content | Factor:intervention RAW_FILE_NAME=Wangyaru-P220801110.mzML SUBJECT_SAMPLE_FACTORS - 122MN Sample source:cecum content | Factor:intervention RAW_FILE_NAME=Wangyaru-P220801111.mzML SUBJECT_SAMPLE_FACTORS - 180MN Sample source:cecum content | Factor:intervention RAW_FILE_NAME=Wangyaru-P220801106.mzML SUBJECT_SAMPLE_FACTORS - 81MN Sample source:cecum content | Factor:intervention RAW_FILE_NAME=Wangyaru-P220801108.mzML SUBJECT_SAMPLE_FACTORS - 907MN Sample source:cecum content | Factor:intervention RAW_FILE_NAME=Wangyaru-P220801107.mzML #COLLECTION CO:COLLECTION_SUMMARY Cecum content was collected and stored at -80°C environment for tryptophan CO:COLLECTION_SUMMARY analysis. CO:SAMPLE_TYPE Cecum CO:STORAGE_CONDITIONS -80℃ #TREATMENT TR:TREATMENT_SUMMARY Mice were randomly assigned into two experimental groups, named control and TR:TREATMENT_SUMMARY intervention groups, receiving standard chow feeding, and standard chow + L. TR:TREATMENT_SUMMARY plantarum. Probiotic was fed at a dose of 1*10^9 CFU/ml/kg body weight in PBS TR:TREATMENT_SUMMARY solution by oral gavage (2 days/week, p.o., three weeks). #SAMPLEPREP SP:SAMPLEPREP_SUMMARY Sample was extracted in 100 μL of 80% methanol aqueous solution, high SP:SAMPLEPREP_SUMMARY throughput tissue grinder grinding for 60 s, then add 900 μL of 10% methanol SP:SAMPLEPREP_SUMMARY aqueous solution, high throughput tissue grinder grinding for 120 s. SP:SAMPLEPREP_SUMMARY Centrifugation was carried out at 12,000 rpm at 4 ℃ for 10 min, and the SP:SAMPLEPREP_SUMMARY supernatant was filtered through the membrane to add an equal amount of the SP:SAMPLEPREP_SUMMARY internal standard solution, and then transferred into the vial. SP:PROCESSING_STORAGE_CONDITIONS 4℃ SP:EXTRACT_STORAGE -80℃ #CHROMATOGRAPHY CH:INSTRUMENT_NAME Thermo Vanquish CH:COLUMN_NAME Waters ACQUITY UPLC HSS T3 (150 x 2.1mm,1.8um) CH:COLUMN_TEMPERATURE 40 CH:FLOW_GRADIENT 0-2 min, 1% B; 2-3 min, 1-30% B; 3-3.5 min, 30% B; 4.5-8 min, 30-50% B; 8-10 CH:FLOW_GRADIENT min, 50-95% B; 10-11 min, 95% B; 11-17 min, 95-1% B. CH:FLOW_RATE 0.3 mL/min CH:SOLVENT_A 0.1% formic acid in water CH:SOLVENT_B 0.1% formic acid in methanol CH:CHROMATOGRAPHY_TYPE LC #ANALYSIS AN:ANALYSIS_TYPE MS #MS MS:INSTRUMENT_NAME ABI Sciex 5000 MS:INSTRUMENT_TYPE Triple quadrupole MS:MS_TYPE ESI MS:MS_COMMENTS Ion Source Temperature 500°C, Ion Source Voltage 5500 V, Collision Gas 6 psi, MS:MS_COMMENTS Curtain Gas 30 psi, Atomizing Gas and Auxiliary Gas both 50 psi. 45 arbitrary MS:MS_COMMENTS units (AU), 13 AU, 1 AU, 350 °C, and 350 °C for sheath gas, aux gas, sweep MS:MS_COMMENTS gas, ion transfer tube, and vaporizer temperature, respectively. The ion source MS:MS_COMMENTS was operated using heated ESI with an ion spray voltage set at 5500 V in MS:MS_COMMENTS positive ion mode. Polarity switching and scheduled selected reaction monitoring MS:MS_COMMENTS (SRM) were employed.The raw data were firstly converted to mzXML format by MS:MS_COMMENTS MSConvert in the ProteoWizard software package (v3.0.8789) (Adusumilli et al., MS:MS_COMMENTS 2017) and processed using XCMS (Domingo-Almenara et al., 2020) for feature MS:MS_COMMENTS detection, retention time correction and alignment. MS:ION_MODE POSITIVE #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS μg/g MS_METABOLITE_DATA_START Samples 185MN 188MN 908MN 909MN 107MN 120MN 122MN 180MN 81MN 907MN Factors Sample source:cecum content | Factor:control Sample source:cecum content | Factor:control Sample source:cecum content | Factor:control Sample source:cecum content | Factor:control Sample source:cecum content | Factor:intervention Sample source:cecum content | Factor:intervention Sample source:cecum content | Factor:intervention Sample source:cecum content | Factor:intervention Sample source:cecum content | Factor:intervention Sample source:cecum content | Factor:intervention 2AP 0.0359 0.0194 0.0325 0.0235 0.0502 0.0395 0.0166 0.0324 0.0193 0.0185 3-HAA 3MI 5-HAA 5-HIAA 0.0630 0.0518 0.0385 0.0698 0.0813 0.0502 0.0254 0.0510 0.0425 0.0484 5HTP 0.0461 0.0277 0.0397 0.0402 0.0263 0.0158 0.0238 0.0204 0.0251 HK I3CA 0.0369 0.0377 0.0428 0.0287 0.0260 0.0162 0.0325 0.0445 0.0282 0.0238 IAA 3.3670 2.5368 3.8736 2.3846 1.4176 1.0626 2.7102 2.3369 2.2114 1.8243 IAld 0.6622 0.5671 0.8237 0.5199 0.2654 0.2048 0.4954 0.5020 0.4365 0.3935 IAM 0.0441 0.0043 0.0048 0.0031 0.0046 0.0032 0.0076 0.0056 0.0060 0.0023 IArA 0.0090 0.0081 0.0359 0.0201 0.0067 0.0063 0.0049 0.0156 0.0047 0.0095 IEt 0.1219 0.1304 0.1185 0.1176 0.0876 0.0996 0.1101 0.1060 0.1240 ILA 0.0866 0.2095 0.1703 0.1160 0.1103 0.0656 0.2565 0.1149 0.1458 0.1563 IND 34.9188 29.9543 44.1049 29.1790 12.6877 9.3402 24.6394 26.5662 22.3473 20.9372 IPA 0.0443 0.0318 0.2868 0.0604 0.0240 0.0164 0.0456 0.0597 0.0142 0.0340 Kyn 0.2194 0.1730 0.2412 0.2113 0.1259 0.0804 0.2153 0.1820 0.1395 0.1312 KynA 2.3395 2.0306 2.5516 1.8084 2.0667 1.4271 2.1415 2.5352 2.0654 1.4845 MEL MeOTA MT Na 34.3274 31.7090 33.6468 22.2485 30.0503 23.6443 28.5242 24.9533 27.7214 22.1830 Nam 0.0081 0.0037 0.0059 0.0132 0.0138 0.0108 0.0076 0.0048 0.0091 0.0017 NAS 0.0013 0.0022 0.0017 0.0014 0.0016 0.0014 0.0013 0.0022 0.0015 0.0014 PA QA Ser 0.0574 0.0277 0.0229 0.0541 0.0643 0.0525 0.0076 0.0248 0.0090 0.0331 TRM 0.0144 0.0242 0.0147 0.0155 0.0130 0.0085 0.0092 0.0079 0.0097 Trp 16.2071 18.7592 22.7847 12.0060 17.7246 24.0606 14.6303 16.3245 17.1582 16.2859 XA 0.1319 0.2271 0.2689 0.1933 0.1315 0.0909 0.0745 0.3019 0.1341 0.1400 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name pubchem_id inchi_key kegg_id other_id other_id_type ri ri_type moverz_quant 2AP 0 1.95 110.053 3-HAA 0 5.4 154.09 3MI 0 10.8 132.071 5-HAA 0 1.72 154.082 5-HIAA 0 6.22 192.048 5HTP 0 3.19 221.106 HK 0 2.05 225.088 I3CA 0 9.29 162.069 IAA 0 9.66 176.087 IAld 0 9.26 146.12 IAM 0 7.62 175.054 IArA 0 10.2 188.023 IEt 0 9.62 162.057 ILA 0 8.95 206.081 IND 0 9.29 118.273 IPA 0 10.2 189.993 Kyn 0 3.63 209.089 KynA 0 6.78 190.031 MEL 0 9.33 233.159 MeOTA 0 6.11 191.252 MT 0 6.9 219.102 Na 0 1.82 124.021 Nam 0 1.86 123.057 NAS 0 6.41 219.108 PA 0 1.56 124.091 QA 0 1.83 168.046 Ser 0 2.83 177.122 TRM 0 5.87 161.053 Trp 0 5.86 205.037 XA 0 6.48 206.054 METABOLITES_END #END