#METABOLOMICS WORKBENCH NuriaCG_20240222_040502 DATATRACK_ID:4668 STUDY_ID:ST003135 ANALYSIS_ID:AN005145 PROJECT_ID:PR001948
VERSION             	1
CREATED_ON             	February 29, 2024, 2:04 am
#PROJECT
PR:PROJECT_TITLE                 	Metabolomic study of T effector and T regulatory cells in severe allergic
PR:PROJECT_TITLE                 	patients
PR:PROJECT_TYPE                  	MS untargeted analysis combining lipidomics and metabolomics.
PR:PROJECT_SUMMARY               	Metabolism has a profound impact on T cell fate and function. Uncovering the
PR:PROJECT_SUMMARY               	metabolome of circulating human CD4+ T effector memory (Teff) and T regulatory
PR:PROJECT_SUMMARY               	(Treg) cells would enable better understanding of Th2-driven diseases, such as
PR:PROJECT_SUMMARY               	allergy or asthma. Here, we demonstrated that in healthy humans, energy
PR:PROJECT_SUMMARY               	metabolism and functions of memory CD4+ Teff cells mainly relied on amino acids,
PR:PROJECT_SUMMARY               	whereas Treg cells predominantly used fatty acids. Arginine and phenylalanine
PR:PROJECT_SUMMARY               	increased T cell receptorinduced glycolysis and oxidative phosphorylation in
PR:PROJECT_SUMMARY               	total and memory CD4+ T cells, but high levels of phenylalanine limited CD4+ T
PR:PROJECT_SUMMARY               	cell proliferation via disrupting mitochondrial respiration and activation of
PR:PROJECT_SUMMARY               	L-phenylalanine oxidase, IL4I1. Accordingly, lowest levels of phenylalanine were
PR:PROJECT_SUMMARY               	linked with the pathogenic Th2a cells, and impaired Treg cells in patients with
PR:PROJECT_SUMMARY               	the most severe forms of allergies. It all suggests that phenylalanine is a
PR:PROJECT_SUMMARY               	metabolic checkpoint of pathogenic Th2 cells development.
PR:INSTITUTE                     	Universidad San Pablo-CEU, CEU Universities
PR:DEPARTMENT                    	Química y Bioquímica
PR:LAST_NAME                     	Villaseñor
PR:FIRST_NAME                    	Alma
PR:ADDRESS                       	Urbanización Montepríncipe, n/s, Boadilla del Monte, Madrid, 28003, Spain
PR:EMAIL                         	alma.villasenor@ceu.es
PR:PHONE                         	913724750
#STUDY
ST:STUDY_TITLE                   	Metabolomic study of T effector and T regulatory cells in severe allergic
ST:STUDY_TITLE                   	patients
ST:STUDY_SUMMARY                 	Metabolism has a profound impact on T cell fate and function. Uncovering the
ST:STUDY_SUMMARY                 	metabolome of circulating human CD4+ T effector memory (Teff) and T regulatory
ST:STUDY_SUMMARY                 	(Treg) cells would enable better understanding of Th2-driven diseases, such as
ST:STUDY_SUMMARY                 	allergy or asthma. Here, we demonstrated that in healthy humans, energy
ST:STUDY_SUMMARY                 	metabolism and functions of memory CD4+ Teff cells mainly relied on amino acids,
ST:STUDY_SUMMARY                 	whereas Treg cells predominantly used fatty acids. Arginine and phenylalanine
ST:STUDY_SUMMARY                 	increased T cell receptorinduced glycolysis and oxidative phosphorylation in
ST:STUDY_SUMMARY                 	total and memory CD4+ T cells, but high levels of phenylalanine limited CD4+ T
ST:STUDY_SUMMARY                 	cell proliferation via disrupting mitochondrial respiration and activation of
ST:STUDY_SUMMARY                 	L-phenylalanine oxidase, IL4I1. Accordingly, lowest levels of phenylalanine were
ST:STUDY_SUMMARY                 	linked with the pathogenic Th2a cells, and impaired Treg cells in patients with
ST:STUDY_SUMMARY                 	the most severe forms of allergies. It all suggests that phenylalanine is a
ST:STUDY_SUMMARY                 	metabolic checkpoint of pathogenic Th2 cells development.
ST:INSTITUTE                     	Universidad San Pablo-CEU, CEU Universities
ST:DEPARTMENT                    	Química y Bioquímica
ST:LAST_NAME                     	Villaseñor
ST:FIRST_NAME                    	Alma
ST:ADDRESS                       	Urbanización Montepríncipe, n/s, Boadilla del Monte, Madrid, 28003, Spain
ST:EMAIL                         	alma.villasenor@ceu.es
ST:PHONE                         	913724750
#SUBJECT
SU:SUBJECT_TYPE                  	Human
SU:SUBJECT_SPECIES               	Homo sapiens
SU:TAXONOMY_ID                   	9606
SU:AGE_OR_AGE_RANGE              	Adult patients (>18 years old)
SU:GENDER                        	Male and female
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data
SUBJECT_SAMPLE_FACTORS           	A2	A2_Eff_Polar	Sample source:PBMCs | Experimental_Group:Non_Allergic | Cell_Type:Teff | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=A2_Eff_Polar_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A2_Eff_Polar_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A2_Eff_Polar_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A2_Eff_Polar_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	A2	A2_Reg_Polar	Sample source:PBMCs | Experimental_Group:Non_Allergic | Cell_Type:Treg | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=A2_Reg_Polar_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A2_Reg_Polar_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A2_Reg_Polar_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A2_Reg_Polar_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	A2	A2_Eff_Lipids	Sample source:PBMCs | Experimental_Group:Non_Allergic | Cell_Type:Teff | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=A2_Eff_Lipids_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A2_Eff_Lipids_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A2_Eff_Lipids_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A2_Eff_Lipids_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	A2	A2_Reg_Lipids	Sample source:PBMCs | Experimental_Group:Non_Allergic | Cell_Type:Treg | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=A2_Reg_Lipids_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A2_Reg_Lipids_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A2_Reg_Lipids_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A2_Reg_Lipids_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	A4	A4_Eff_Polar	Sample source:PBMCs | Experimental_Group:Non_Allergic | Cell_Type:Teff | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=A4_Eff_Polar_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A4_Eff_Polar_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A4_Eff_Polar_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A4_Eff_Polar_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	A4	A4_Reg_Polar	Sample source:PBMCs | Experimental_Group:Non_Allergic | Cell_Type:Treg | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=A4_Reg_Polar_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A4_Reg_Polar_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A4_Reg_Polar_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A4_Reg_Polar_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	A4	A4_Eff_Lipids	Sample source:PBMCs | Experimental_Group:Non_Allergic | Cell_Type:Teff | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=A4_Eff_Lipids_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A4_Eff_Lipids_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A4_Eff_Lipids_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A4_Eff_Lipids_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	A4	A4_Reg_Lipids	Sample source:PBMCs | Experimental_Group:Non_Allergic | Cell_Type:Treg | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=A4_Reg_Lipids_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A4_Reg_Lipids_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A4_Reg_Lipids_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A4_Reg_Lipids_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	A5	A5_Eff_Polar	Sample source:PBMCs | Experimental_Group:Non_Allergic | Cell_Type:Teff | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=A5_Eff_Polar_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A5_Eff_Polar_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A5_Eff_Polar_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A5_Eff_Polar_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	A5	A5_Reg_Polar	Sample source:PBMCs | Experimental_Group:Non_Allergic | Cell_Type:Treg | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=A5_Reg_Polar_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A5_Reg_Polar_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A5_Reg_Polar_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A5_Reg_Polar_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	A5	A5_Eff_Lipids	Sample source:PBMCs | Experimental_Group:Non_Allergic | Cell_Type:Teff | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=A5_Eff_Lipids_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A5_Eff_Lipids_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A5_Eff_Lipids_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A5_Eff_Lipids_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	A5	A5_Reg_Lipids	Sample source:PBMCs | Experimental_Group:Non_Allergic | Cell_Type:Treg | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=A5_Reg_Lipids_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A5_Reg_Lipids_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A5_Reg_Lipids_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A5_Reg_Lipids_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	A6	A6_Eff_Polar	Sample source:PBMCs | Experimental_Group:Non_Allergic | Cell_Type:Teff | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=A6_Eff_Polar_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A6_Eff_Polar_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A6_Eff_Polar_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A6_Eff_Polar_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	A6	A6_Reg_Polar	Sample source:PBMCs | Experimental_Group:Non_Allergic | Cell_Type:Treg | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=A6_Reg_Polar_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A6_Reg_Polar_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A6_Reg_Polar_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A6_Reg_Polar_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	A6	A6_Eff_Lipids	Sample source:PBMCs | Experimental_Group:Non_Allergic | Cell_Type:Teff | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=A6_Eff_Lipids_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A6_Eff_Lipids_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A6_Eff_Lipids_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A6_Eff_Lipids_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	A6	A6_Reg_Lipids	Sample source:PBMCs | Experimental_Group:Non_Allergic | Cell_Type:Treg | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=A6_Reg_Lipids_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A6_Reg_Lipids_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A6_Reg_Lipids_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A6_Reg_Lipids_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	A7	A7_Eff_Polar	Sample source:PBMCs | Experimental_Group:Non_Allergic | Cell_Type:Teff | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=A7_Eff_Polar_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A7_Eff_Polar_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A7_Eff_Polar_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A7_Eff_Polar_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	A7	A7_Reg_Polar	Sample source:PBMCs | Experimental_Group:Non_Allergic | Cell_Type:Treg | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=A7_Reg_Polar_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A7_Reg_Polar_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A7_Reg_Polar_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A7_Reg_Polar_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	A7	A7_Eff_Lipids	Sample source:PBMCs | Experimental_Group:Non_Allergic | Cell_Type:Teff | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=A7_Eff_Lipids_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A7_Eff_Lipids_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A7_Eff_Lipids_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A7_Eff_Lipids_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	A7	A7_Reg_Lipids	Sample source:PBMCs | Experimental_Group:Non_Allergic | Cell_Type:Treg | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=A7_Reg_Lipids_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A7_Reg_Lipids_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A7_Reg_Lipids_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A7_Reg_Lipids_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	A8	A8_Eff_Polar	Sample source:PBMCs | Experimental_Group:Non_Allergic | Cell_Type:Teff | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=A8_Eff_Polar_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A8_Eff_Polar_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A8_Eff_Polar_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A8_Eff_Polar_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	A8	A8_Reg_Polar	Sample source:PBMCs | Experimental_Group:Non_Allergic | Cell_Type:Treg | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=A8_Reg_Polar_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A8_Reg_Polar_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A8_Reg_Polar_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A8_Reg_Polar_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	A8	A8_Eff_Lipids	Sample source:PBMCs | Experimental_Group:Non_Allergic | Cell_Type:Teff | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=A8_Eff_Lipids_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A8_Eff_Lipids_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A8_Eff_Lipids_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A8_Eff_Lipids_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	A8	A8_Reg_Lipids	Sample source:PBMCs | Experimental_Group:Non_Allergic | Cell_Type:Treg | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=A8_Reg_Lipids_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A8_Reg_Lipids_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A8_Reg_Lipids_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A8_Reg_Lipids_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	B2	B2_Eff_Polar	Sample source:PBMCs | Experimental_Group:Mild | Cell_Type:Teff | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=B2_Eff_Polar_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=B2_Eff_Polar_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=B2_Eff_Polar_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=B2_Eff_Polar_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	B2	B2_Reg_Polar	Sample source:PBMCs | Experimental_Group:Mild | Cell_Type:Treg | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=B2_Reg_Polar_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=B2_Reg_Polar_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=B2_Reg_Polar_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=B2_Reg_Polar_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	B2	B2_Eff_Lipids	Sample source:PBMCs | Experimental_Group:Mild | Cell_Type:Teff | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=B2_Eff_Lipids_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=B2_Eff_Lipids_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=B2_Eff_Lipids_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=B2_Eff_Lipids_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	B2	B2_Reg_Lipids	Sample source:PBMCs | Experimental_Group:Mild | Cell_Type:Treg | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=B2_Reg_Lipids_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=B2_Reg_Lipids_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=B2_Reg_Lipids_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=B2_Reg_Lipids_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	B3	B3_Eff_Polar	Sample source:PBMCs | Experimental_Group:Mild | Cell_Type:Teff | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=B3_Eff_Polar_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=B3_Eff_Polar_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=B3_Eff_Polar_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=B3_Eff_Polar_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	B3	B3_Reg_Polar	Sample source:PBMCs | Experimental_Group:Mild | Cell_Type:Treg | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=B3_Reg_Polar_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=B3_Reg_Polar_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=B3_Reg_Polar_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=B3_Reg_Polar_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	B3	B3_Eff_Lipids	Sample source:PBMCs | Experimental_Group:Mild | Cell_Type:Teff | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=B3_Eff_Lipids_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=B3_Eff_Lipids_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=B3_Eff_Lipids_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=B3_Eff_Lipids_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	B3	B3_Reg_Lipids	Sample source:PBMCs | Experimental_Group:Mild | Cell_Type:Treg | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=B3_Reg_Lipids_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=B3_Reg_Lipids_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=B3_Reg_Lipids_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=B3_Reg_Lipids_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	B4	B4_Eff_Polar	Sample source:PBMCs | Experimental_Group:Mild | Cell_Type:Teff | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=B4_Eff_Polar_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=B4_Eff_Polar_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=B4_Eff_Polar_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=B4_Eff_Polar_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	B4	B4_Reg_Polar	Sample source:PBMCs | Experimental_Group:Mild | Cell_Type:Treg | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=B4_Reg_Polar_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=B4_Reg_Polar_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=B4_Reg_Polar_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=B4_Reg_Polar_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	B4	B4_Eff_Lipids	Sample source:PBMCs | Experimental_Group:Mild | Cell_Type:Teff | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=B4_Eff_Lipids_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=B4_Eff_Lipids_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=B4_Eff_Lipids_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=B4_Eff_Lipids_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	B4	B4_Reg_Lipids	Sample source:PBMCs | Experimental_Group:Mild | Cell_Type:Treg | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=B4_Reg_Lipids_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=B4_Reg_Lipids_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=B4_Reg_Lipids_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=B4_Reg_Lipids_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	C1	C1_Eff_Polar	Sample source:PBMCs | Experimental_Group:Severe | Cell_Type:Teff | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=C1_Eff_Polar_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C1_Eff_Polar_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C1_Eff_Polar_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C1_Eff_Polar_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	C1	C1_Reg_Polar	Sample source:PBMCs | Experimental_Group:Severe | Cell_Type:Treg | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=C1_Reg_Polar_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C1_Reg_Polar_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C1_Reg_Polar_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C1_Reg_Polar_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	C1	C1_Eff_Lipids	Sample source:PBMCs | Experimental_Group:Severe | Cell_Type:Teff | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=C1_Eff_Lipids_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C1_Eff_Lipids_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C1_Eff_Lipids_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C1_Eff_Lipids_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	C1	C1_Reg_Lipids	Sample source:PBMCs | Experimental_Group:Severe | Cell_Type:Treg | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=C1_Reg_Lipids_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C1_Reg_Lipids_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C1_Reg_Lipids_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C1_Reg_Lipids_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	C2	C2_Eff_Polar	Sample source:PBMCs | Experimental_Group:Severe | Cell_Type:Teff | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=C2_Eff_Polar_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C2_Eff_Polar_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C2_Eff_Polar_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C2_Eff_Polar_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	C2	C2_Reg_Polar	Sample source:PBMCs | Experimental_Group:Severe | Cell_Type:Treg | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=C2_Reg_Polar_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C2_Reg_Polar_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C2_Reg_Polar_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C2_Reg_Polar_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	C2	C2_Eff_Lipids	Sample source:PBMCs | Experimental_Group:Severe | Cell_Type:Teff | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=C2_Eff_Lipids_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C2_Eff_Lipids_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C2_Eff_Lipids_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C2_Eff_Lipids_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	C2	C2_Reg_Lipids	Sample source:PBMCs | Experimental_Group:Severe | Cell_Type:Treg | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=C2_Reg_Lipids_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C2_Reg_Lipids_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C2_Reg_Lipids_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C2_Reg_Lipids_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	C6	C6_Eff_Polar	Sample source:PBMCs | Experimental_Group:Severe | Cell_Type:Teff | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=C6_Eff_Polar_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C6_Eff_Polar_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C6_Eff_Polar_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C6_Eff_Polar_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	C6	C6_Reg_Polar	Sample source:PBMCs | Experimental_Group:Severe | Cell_Type:Treg | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=C6_Reg_Polar_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C6_Reg_Polar_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C6_Reg_Polar_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C6_Reg_Polar_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	C6	C6_Eff_Lipids	Sample source:PBMCs | Experimental_Group:Severe | Cell_Type:Teff | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=C6_Eff_Lipids_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C6_Eff_Lipids_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C6_Eff_Lipids_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C6_Eff_Lipids_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	C6	C6_Reg_Lipids	Sample source:PBMCs | Experimental_Group:Severe | Cell_Type:Treg | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=C6_Reg_Lipids_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C6_Reg_Lipids_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C6_Reg_Lipids_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C6_Reg_Lipids_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	C7	C7_Eff_Polar	Sample source:PBMCs | Experimental_Group:Severe | Cell_Type:Teff | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=C7_Eff_Polar_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C7_Eff_Polar_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C7_Eff_Polar_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C7_Eff_Polar_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	C7	C7_Reg_Polar	Sample source:PBMCs | Experimental_Group:Severe | Cell_Type:Treg | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=C7_Reg_Polar_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C7_Reg_Polar_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C7_Reg_Polar_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C7_Reg_Polar_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	C7	C7_Eff_Lipids	Sample source:PBMCs | Experimental_Group:Severe | Cell_Type:Teff | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=C7_Eff_Lipids_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C7_Eff_Lipids_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C7_Eff_Lipids_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C7_Eff_Lipids_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	C7	C7_Reg_Lipids	Sample source:PBMCs | Experimental_Group:Severe | Cell_Type:Treg | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=C7_Reg_Lipids_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C7_Reg_Lipids_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C7_Reg_Lipids_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C7_Reg_Lipids_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	C8	C8_Eff_Polar	Sample source:PBMCs | Experimental_Group:Severe | Cell_Type:Teff | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=C8_Eff_Polar_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C8_Eff_Polar_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C8_Eff_Polar_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C8_Eff_Polar_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	C8	C8_Reg_Polar	Sample source:PBMCs | Experimental_Group:Severe | Cell_Type:Treg | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=C8_Reg_Polar_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C8_Reg_Polar_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C8_Reg_Polar_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C8_Reg_Polar_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	C8	C8_Eff_Lipids	Sample source:PBMCs | Experimental_Group:Severe | Cell_Type:Teff | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=C8_Eff_Lipids_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C8_Eff_Lipids_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C8_Eff_Lipids_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C8_Eff_Lipids_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	C8	C8_Reg_Lipids	Sample source:PBMCs | Experimental_Group:Severe | Cell_Type:Treg | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=C8_Reg_Lipids_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C8_Reg_Lipids_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C8_Reg_Lipids_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C8_Reg_Lipids_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	C9	C9_Eff_Polar	Sample source:PBMCs | Experimental_Group:Severe | Cell_Type:Teff | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=C9_Eff_Polar_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C9_Eff_Polar_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C9_Eff_Polar_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C9_Eff_Polar_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	C9	C9_Reg_Polar	Sample source:PBMCs | Experimental_Group:Severe | Cell_Type:Treg | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=C9_Reg_Polar_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C9_Reg_Polar_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C9_Reg_Polar_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C9_Reg_Polar_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	C9	C9_Eff_Lipids	Sample source:PBMCs | Experimental_Group:Severe | Cell_Type:Teff | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=C9_Eff_Lipids_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C9_Eff_Lipids_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C9_Eff_Lipids_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C9_Eff_Lipids_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	C9	C9_Reg_Lipids	Sample source:PBMCs | Experimental_Group:Severe | Cell_Type:Treg | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=C9_Reg_Lipids_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C9_Reg_Lipids_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C9_Reg_Lipids_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C9_Reg_Lipids_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	C10	C10_Eff_Polar	Sample source:PBMCs | Experimental_Group:Severe | Cell_Type:Teff | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=C10_Eff_Polar_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C10_Eff_Polar_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C10_Eff_Polar_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C10_Eff_Polar_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	C10	C10_Reg_Polar	Sample source:PBMCs | Experimental_Group:Severe | Cell_Type:Treg | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=C10_Reg_Polar_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C10_Reg_Polar_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C10_Reg_Polar_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C10_Reg_Polar_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	C10	C10_Eff_Lipids	Sample source:PBMCs | Experimental_Group:Severe | Cell_Type:Teff | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=C10_Eff_Lipids_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C10_Eff_Lipids_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C10_Eff_Lipids_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C10_Eff_Lipids_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	C10	C10_Reg_Lipids	Sample source:PBMCs | Experimental_Group:Severe | Cell_Type:Treg | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=C10_Reg_Lipids_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C10_Reg_Lipids_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C10_Reg_Lipids_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C10_Reg_Lipids_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	C11	C11_Eff_Polar	Sample source:PBMCs | Experimental_Group:Severe | Cell_Type:Teff | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=C11_Eff_Polar_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C11_Eff_Polar_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C11_Eff_Polar_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C11_Eff_Polar_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	C11	C11_Reg_Polar	Sample source:PBMCs | Experimental_Group:Severe | Cell_Type:Treg | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=C11_Reg_Polar_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C11_Reg_Polar_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C11_Reg_Polar_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C11_Reg_Polar_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	C11	C11_Eff_Lipids	Sample source:PBMCs | Experimental_Group:Severe | Cell_Type:Teff | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=C11_Eff_Lipids_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C11_Eff_Lipids_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C11_Eff_Lipids_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C11_Eff_Lipids_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	C11	C11_Reg_Lipids	Sample source:PBMCs | Experimental_Group:Severe | Cell_Type:Treg | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=C11_Reg_Lipids_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C11_Reg_Lipids_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C11_Reg_Lipids_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C11_Reg_Lipids_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	C12	C12_Eff_Polar	Sample source:PBMCs | Experimental_Group:Severe | Cell_Type:Teff | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=C12_Eff_Polar_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C12_Eff_Polar_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C12_Eff_Polar_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C12_Eff_Polar_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	C12	C12_Reg_Polar	Sample source:PBMCs | Experimental_Group:Severe | Cell_Type:Treg | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=C12_Reg_Polar_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C12_Reg_Polar_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C12_Reg_Polar_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C12_Reg_Polar_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	C12	C12_Eff_Lipids	Sample source:PBMCs | Experimental_Group:Severe | Cell_Type:Teff | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=C12_Eff_Lipids_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C12_Eff_Lipids_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C12_Eff_Lipids_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C12_Eff_Lipids_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	C12	C12_Reg_Lipids	Sample source:PBMCs | Experimental_Group:Severe | Cell_Type:Treg | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=C12_Reg_Lipids_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C12_Reg_Lipids_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C12_Reg_Lipids_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C12_Reg_Lipids_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	C13	C13_Eff_Polar	Sample source:PBMCs | Experimental_Group:Severe | Cell_Type:Teff | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=C13_Eff_Polar_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C13_Eff_Polar_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C13_Eff_Polar_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C13_Eff_Polar_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	C13	C13_Reg_Polar	Sample source:PBMCs | Experimental_Group:Severe | Cell_Type:Treg | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=C13_Reg_Polar_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C13_Reg_Polar_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C13_Reg_Polar_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C13_Reg_Polar_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	C13	C13_Eff_Lipids	Sample source:PBMCs | Experimental_Group:Severe | Cell_Type:Teff | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=C13_Eff_Lipids_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C13_Eff_Lipids_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C13_Eff_Lipids_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C13_Eff_Lipids_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	C13	C13_Reg_Lipids	Sample source:PBMCs | Experimental_Group:Severe | Cell_Type:Treg | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=C13_Reg_Lipids_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C13_Reg_Lipids_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C13_Reg_Lipids_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C13_Reg_Lipids_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	QC1	QC1_Reg_Polar	Sample source:PBMCs | Experimental_Group:Quality_Control | Cell_Type:Treg | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=QC1_Reg_Polar_Pos.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=QC1_Reg_Polar_Neg.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	QC1	QC1_Reg_Lipids	Sample source:PBMCs | Experimental_Group:Quality_Control | Cell_Type:Treg | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=QC1_Reg_Lipids_Pos.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=QC1_Reg_Lipids_Neg.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	QC2	QC2_Reg_Polar	Sample source:PBMCs | Experimental_Group:Quality_Control | Cell_Type:Treg | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=QC2_Reg_Polar_Pos.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=QC2_Reg_Polar_Neg.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	QC2	QC2_Reg_Lipids	Sample source:PBMCs | Experimental_Group:Quality_Control | Cell_Type:Treg | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=QC2_Reg_Lipids_Pos.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=QC2_Reg_Lipids_Neg.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	QC3	QC3_Reg_Polar	Sample source:PBMCs | Experimental_Group:Quality_Control | Cell_Type:Treg | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=QC3_Reg_Polar_Pos.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=QC3_Reg_Polar_Neg.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	QC3	QC3_Reg_Lipids	Sample source:PBMCs | Experimental_Group:Quality_Control | Cell_Type:Treg | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=QC3_Reg_Lipids_Pos.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=QC3_Reg_Lipids_Neg.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	QC4	QC4_Reg_Polar	Sample source:PBMCs | Experimental_Group:Quality_Control | Cell_Type:Treg | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=QC4_Reg_Polar_Pos.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=QC4_Reg_Polar_Neg.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	QC4	QC4_Reg_Lipids	Sample source:PBMCs | Experimental_Group:Quality_Control | Cell_Type:Treg | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=QC4_Reg_Lipids_Pos.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=QC4_Reg_Lipids_Neg.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	QC5	QC5_Reg_Polar	Sample source:PBMCs | Experimental_Group:Quality_Control | Cell_Type:Treg | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=QC5_Reg_Polar_Pos.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=QC5_Reg_Polar_Neg.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	QC5	QC5_Reg_Lipids	Sample source:PBMCs | Experimental_Group:Quality_Control | Cell_Type:Treg | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=QC5_Reg_Lipids_Pos.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=QC5_Reg_Lipids_Neg.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	QC6	QC6_Reg_Polar	Sample source:PBMCs | Experimental_Group:Quality_Control | Cell_Type:Treg | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=QC6_Reg_Polar_Pos.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=QC6_Reg_Polar_Neg.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	QC6	QC6_Reg_Lipids	Sample source:PBMCs | Experimental_Group:Quality_Control | Cell_Type:Treg | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=QC6_Reg_Lipids_Pos.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=QC6_Reg_Lipids_Neg.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	QC7	QC7_Reg_Polar	Sample source:PBMCs | Experimental_Group:Quality_Control | Cell_Type:Treg | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=QC7_Reg_Polar_Pos.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=QC7_Reg_Polar_Neg.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	QC7	QC7_Reg_Lipids	Sample source:PBMCs | Experimental_Group:Quality_Control | Cell_Type:Treg | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=QC7_Reg_Lipids_Pos.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=QC7_Reg_Lipids_Neg.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	QC8	QC8_Reg_Polar	Sample source:PBMCs | Experimental_Group:Quality_Control | Cell_Type:Treg | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=QC8_Reg_Polar_Pos.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=QC8_Reg_Polar_Neg.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	QC8	QC8_Reg_Lipids	Sample source:PBMCs | Experimental_Group:Quality_Control | Cell_Type:Treg | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=QC8_Reg_Lipids_Pos.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=QC8_Reg_Lipids_Neg.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	QC9	QC9_Reg_Polar	Sample source:PBMCs | Experimental_Group:Quality_Control | Cell_Type:Treg | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=QC9_Reg_Polar_Pos.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=QC9_Reg_Polar_Neg.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	QC9	QC9_Reg_Lipids	Sample source:PBMCs | Experimental_Group:Quality_Control | Cell_Type:Treg | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=QC9_Reg_Lipids_Pos.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=QC9_Reg_Lipids_Neg.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	QC10	QC10_Reg_Polar	Sample source:PBMCs | Experimental_Group:Quality_Control | Cell_Type:Treg | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=QC10_Reg_Polar_Pos.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=QC10_Reg_Polar_Neg.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	QC10	QC10_Reg_Lipids	Sample source:PBMCs | Experimental_Group:Quality_Control | Cell_Type:Treg | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=QC10_Reg_Lipids_Pos.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=QC10_Reg_Lipids_Neg.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	QC12	QC12_Eff_Polar	Sample source:PBMCs | Experimental_Group:Quality_Control | Cell_Type:Teff | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=QC12_Eff_Polar_Pos.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=QC12_Eff_Polar_Neg.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	QC12	QC12_Eff_Lipids	Sample source:PBMCs | Experimental_Group:Quality_Control | Cell_Type:Teff | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=QC12_Eff_Lipids_Pos.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=QC12_Eff_Lipids_Neg.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	QC13	QC13_Eff_Polar	Sample source:PBMCs | Experimental_Group:Quality_Control | Cell_Type:Teff | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=QC13_Eff_Polar_Pos.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=QC13_Eff_Polar_Neg.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	QC13	QC13_Eff_Lipids	Sample source:PBMCs | Experimental_Group:Quality_Control | Cell_Type:Teff | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=QC13_Eff_Lipids_Pos.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=QC13_Eff_Lipids_Neg.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	QC14	QC14_Eff_Polar	Sample source:PBMCs | Experimental_Group:Quality_Control | Cell_Type:Teff | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=QC14_Eff_Polar_Pos.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=QC14_Eff_Polar_Neg.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	QC14	QC14_Eff_Lipids	Sample source:PBMCs | Experimental_Group:Quality_Control | Cell_Type:Teff | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=QC14_Eff_Lipids_Pos.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=QC14_Eff_Lipids_Neg.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	QC15	QC15_Eff_Polar	Sample source:PBMCs | Experimental_Group:Quality_Control | Cell_Type:Teff | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=QC15_Eff_Polar_Pos.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=QC15_Eff_Polar_Neg.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	QC15	QC15_Eff_Lipids	Sample source:PBMCs | Experimental_Group:Quality_Control | Cell_Type:Teff | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=QC15_Eff_Lipids_Pos.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=QC15_Eff_Lipids_Neg.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	QC16	QC16_Eff_Polar	Sample source:PBMCs | Experimental_Group:Quality_Control | Cell_Type:Teff | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=QC16_Eff_Polar_Pos.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=QC16_Eff_Polar_Neg.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	QC16	QC16_Eff_Lipids	Sample source:PBMCs | Experimental_Group:Quality_Control | Cell_Type:Teff | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=QC16_Eff_Lipids_Pos.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=QC16_Eff_Lipids_Neg.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	QC17	QC17_Eff_Polar	Sample source:PBMCs | Experimental_Group:Quality_Control | Cell_Type:Teff | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=QC17_Eff_Polar_Pos.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=QC17_Eff_Polar_Neg.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	QC17	QC17_Eff_Lipids	Sample source:PBMCs | Experimental_Group:Quality_Control | Cell_Type:Teff | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=QC17_Eff_Lipids_Pos.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=QC17_Eff_Lipids_Neg.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	QC18	QC18_Eff_Polar	Sample source:PBMCs | Experimental_Group:Quality_Control | Cell_Type:Teff | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=QC18_Eff_Polar_Pos.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=QC18_Eff_Polar_Neg.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	QC18	QC18_Eff_Lipids	Sample source:PBMCs | Experimental_Group:Quality_Control | Cell_Type:Teff | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=QC18_Eff_Lipids_Pos.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=QC18_Eff_Lipids_Neg.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	QC19	QC19_Eff_Polar	Sample source:PBMCs | Experimental_Group:Quality_Control | Cell_Type:Teff | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=QC19_Eff_Polar_Pos.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=QC19_Eff_Polar_Neg.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	QC19	QC19_Eff_Lipids	Sample source:PBMCs | Experimental_Group:Quality_Control | Cell_Type:Teff | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=QC19_Eff_Lipids_Pos.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=QC19_Eff_Lipids_Neg.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	QC20	QC20_Eff_Polar	Sample source:PBMCs | Experimental_Group:Quality_Control | Cell_Type:Teff | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=QC20_Eff_Polar_Pos.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=QC20_Eff_Polar_Neg.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	QC20	QC20_Eff_Lipids	Sample source:PBMCs | Experimental_Group:Quality_Control | Cell_Type:Teff | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=QC20_Eff_Lipids_Pos.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=QC20_Eff_Lipids_Neg.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	QC21	QC21_Eff_Polar	Sample source:PBMCs | Experimental_Group:Quality_Control | Cell_Type:Teff | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=QC21_Eff_Polar_Pos.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=QC21_Eff_Polar_Neg.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	QC21	QC21_Eff_Lipids	Sample source:PBMCs | Experimental_Group:Quality_Control | Cell_Type:Teff | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=QC21_Eff_Lipids_Pos.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=QC21_Eff_Lipids_Neg.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	Curve_10	Curve_10_Reg_Polar	Sample source:PBMCs | Experimental_Group:Cell_Curve | Cell_Type:Treg | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=Curve_10_Reg_Polar_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_10_Reg_Polar_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_10_Reg_Polar_Pos_r3.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_10_Reg_Polar_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_10_Reg_Polar_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_10_Reg_Polar_Neg_r3.mzML
SUBJECT_SAMPLE_FACTORS           	Curve_10	Curve_10_Reg_Lipids	Sample source:PBMCs | Experimental_Group:Cell_Curve | Cell_Type:Treg | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=Curve_10_Reg_Lipids_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_10_Reg_Lipids_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_10_Reg_Lipids_Pos_r3.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_10_Reg_Lipids_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_10_Reg_Lipids_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_10_Reg_Lipids_Neg_r3.mzML
SUBJECT_SAMPLE_FACTORS           	Curve_25	Curve_25_Reg_Polar	Sample source:PBMCs | Experimental_Group:Cell_Curve | Cell_Type:Treg | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=Curve_25_Reg_Polar_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_25_Reg_Polar_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_25_Reg_Polar_Pos_r3.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_25_Reg_Polar_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_25_Reg_Polar_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_25_Reg_Polar_Neg_r3.mzML
SUBJECT_SAMPLE_FACTORS           	Curve_25	Curve_25_Reg_Lipids	Sample source:PBMCs | Experimental_Group:Cell_Curve | Cell_Type:Treg | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=Curve_25_Reg_Lipids_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_25_Reg_Lipids_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_25_Reg_Lipids_Pos_r3.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_25_Reg_Lipids_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_25_Reg_Lipids_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_25_Reg_Lipids_Neg_r3.mzML
SUBJECT_SAMPLE_FACTORS           	Curve_50	Curve_50_Reg_Polar	Sample source:PBMCs | Experimental_Group:Cell_Curve | Cell_Type:Treg | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=Curve_50_Reg_Polar_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_50_Reg_Polar_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_50_Reg_Polar_Pos_r3.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_50_Reg_Polar_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_50_Reg_Polar_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_50_Reg_Polar_Neg_r3.mzML
SUBJECT_SAMPLE_FACTORS           	Curve_50	Curve_50_Reg_Lipids	Sample source:PBMCs | Experimental_Group:Cell_Curve | Cell_Type:Treg | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=Curve_50_Reg_Lipids_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_50_Reg_Lipids_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_50_Reg_Lipids_Pos_r3.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_50_Reg_Lipids_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_50_Reg_Lipids_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_50_Reg_Lipids_Neg_r3.mzML
SUBJECT_SAMPLE_FACTORS           	Curve_100	Curve_100_Reg_Polar	Sample source:PBMCs | Experimental_Group:Cell_Curve | Cell_Type:Treg | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=Curve_100_Reg_Polar_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_100_Reg_Polar_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_100_Reg_Polar_Pos_r3.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_100_Reg_Polar_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_100_Reg_Polar_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_100_Reg_Polar_Neg_r3.mzML
SUBJECT_SAMPLE_FACTORS           	Curve_100	Curve_100_Reg_Lipids	Sample source:PBMCs | Experimental_Group:Cell_Curve | Cell_Type:Treg | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=Curve_100_Reg_Lipids_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_100_Reg_Lipids_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_100_Reg_Lipids_Pos_r3.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_100_Reg_Lipids_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_100_Reg_Lipids_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_100_Reg_Lipids_Neg_r3.mzML
SUBJECT_SAMPLE_FACTORS           	Curve_100	Curve_100_Eff_Polar	Sample source:PBMCs | Experimental_Group:Cell_Curve | Cell_Type:Teff | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=Curve_100_Eff_Polar_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_100_Eff_Polar_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_100_Eff_Polar_Pos_r3.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_100_Eff_Polar_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_100_Eff_Polar_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_100_Eff_Polar_Neg_r3.mzML
SUBJECT_SAMPLE_FACTORS           	Curve_100	Curve_100_Eff_Lipids	Sample source:PBMCs | Experimental_Group:Cell_Curve | Cell_Type:Teff | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=Curve_100_Eff_Lipids_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_100_Eff_Lipids_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_100_Eff_Lipids_Pos_r3.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_100_Eff_Lipids_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_100_Eff_Lipids_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_100_Eff_Lipids_Neg_r3.mzML
SUBJECT_SAMPLE_FACTORS           	Curve_250	Curve_250_Eff_Polar	Sample source:PBMCs | Experimental_Group:Cell_Curve | Cell_Type:Teff | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=Curve_250_Eff_Polar_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_250_Eff_Polar_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_250_Eff_Polar_Pos_r3.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_250_Eff_Polar_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_250_Eff_Polar_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_250_Eff_Polar_Neg_r3.mzML
SUBJECT_SAMPLE_FACTORS           	Curve_250	Curve_250_Eff_Lipids	Sample source:PBMCs | Experimental_Group:Cell_Curve | Cell_Type:Teff | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=Curve_250_Eff_Lipids_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_250_Eff_Lipids_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_250_Eff_Lipids_Pos_r3.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_250_Eff_Lipids_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_250_Eff_Lipids_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_250_Eff_Lipids_Neg_r3.mzML
SUBJECT_SAMPLE_FACTORS           	Curve_500	Curve_500_Eff_Polar	Sample source:PBMCs | Experimental_Group:Cell_Curve | Cell_Type:Teff | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=Curve_500_Eff_Polar_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_500_Eff_Polar_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_500_Eff_Polar_Pos_r3.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_500_Eff_Polar_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_500_Eff_Polar_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_500_Eff_Polar_Neg_r3.mzML
SUBJECT_SAMPLE_FACTORS           	Curve_500	Curve_500_Eff_Lipids	Sample source:PBMCs | Experimental_Group:Cell_Curve | Cell_Type:Teff | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=Curve_500_Eff_Lipids_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_500_Eff_Lipids_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_500_Eff_Lipids_Pos_r3.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_500_Eff_Lipids_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_500_Eff_Lipids_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_500_Eff_Lipids_Neg_r3.mzML
SUBJECT_SAMPLE_FACTORS           	Curve_1500	Curve_1500_Eff_Polar	Sample source:PBMCs | Experimental_Group:Cell_Curve | Cell_Type:Teff | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=Curve_1500_Eff_Polar_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_1500_Eff_Polar_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_1500_Eff_Polar_Pos_r3.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_1500_Eff_Polar_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_1500_Eff_Polar_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_1500_Eff_Polar_Neg_r3.mzML
SUBJECT_SAMPLE_FACTORS           	Curve_1500	Curve_1500_Eff_Lipids	Sample source:PBMCs | Experimental_Group:Cell_Curve | Cell_Type:Teff | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=Curve_1500_Eff_Lipids_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_1500_Eff_Lipids_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_1500_Eff_Lipids_Pos_r3.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_1500_Eff_Lipids_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_1500_Eff_Lipids_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_1500_Eff_Lipids_Neg_r3.mzML
SUBJECT_SAMPLE_FACTORS           	Blank	Blank_Reg_Polar	Sample source:PBMCs | Experimental_Group:Blank | Cell_Type:Treg | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=Blank_Reg_Polar_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Blank_Reg_Polar_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Blank_Reg_Polar_Pos_r3.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Blank_Reg_Polar_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Blank_Reg_Polar_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Blank_Reg_Polar_Neg_r3.mzML
SUBJECT_SAMPLE_FACTORS           	Blank	Blank_Reg_Lipids	Sample source:PBMCs | Experimental_Group:Blank | Cell_Type:Treg | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=Blank_Reg_Lipids_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Blank_Reg_Lipids_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Blank_Reg_Lipids_Pos_r3.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Blank_Reg_Lipids_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Blank_Reg_Lipids_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Blank_Reg_Lipids_Neg_r3.mzML
SUBJECT_SAMPLE_FACTORS           	Blank	Blank_Eff_Polar	Sample source:PBMCs | Experimental_Group:Blank | Cell_Type:Teff | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=Blank_Eff_Polar_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Blank_Eff_Polar_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Blank_Eff_Polar_Pos_r3.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Blank_Eff_Polar_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Blank_Eff_Polar_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Blank_Eff_Polar_Neg_r3.mzML
SUBJECT_SAMPLE_FACTORS           	Blank	Blank_Eff_Lipids	Sample source:PBMCs | Experimental_Group:Blank | Cell_Type:Teff | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=Blank_Eff_Lipids_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Blank_Eff_Lipids_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Blank_Eff_Lipids_Pos_r3.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Blank_Eff_Lipids_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Blank_Eff_Lipids_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Blank_Eff_Lipids_Neg_r3.mzML
#COLLECTION
CO:COLLECTION_SUMMARY            	Whole blood was drawn from the participants into heparin tubes and serum in
CO:COLLECTION_SUMMARY            	silica tubes. Next, PBMCs were isolated using Ficoll Plaque by gradient density
CO:COLLECTION_SUMMARY            	centrifugation according to manufacturer instructions. Isolated PMBCs were then
CO:COLLECTION_SUMMARY            	immediately frozen and kept at -80˚C until analysis. Teff and Treg cell
CO:COLLECTION_SUMMARY            	subpopulations were determined through FlowJo X (v.10.7.1.) and FlowSOM (v.2.6).
CO:SAMPLE_TYPE                   	Blood (whole)
CO:STORAGE_CONDITIONS            	-80℃
#TREATMENT
TR:TREATMENT_SUMMARY             	After cell sorting, the only treatment received was the addition of a volume of
TR:TREATMENT_SUMMARY             	50 uL of MeOH to each sample and then cells were frozen at -80 ºC until
TR:TREATMENT_SUMMARY             	metabolomics and lipidomic analysis.
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	Frozen cells in MeOH were thawed at 4˚C. Then a first extraction targeted at
SP:SAMPLEPREP_SUMMARY            	lipid metabolites was performed by adding MTBE to a final concentration of MTBE:
SP:SAMPLEPREP_SUMMARY            	MeOH (1:4). Samples were then sonicated for a total of 15 min at 15W (3 rounds
SP:SAMPLEPREP_SUMMARY            	of 5 min sonication), thoroughly vortexed, and centrifuged for 10 min at 16000 g
SP:SAMPLEPREP_SUMMARY            	to remove any debris. The supernatant was collected. Then a second extraction
SP:SAMPLEPREP_SUMMARY            	took place to obtain the most polar metabolites in the sample. To that extent,
SP:SAMPLEPREP_SUMMARY            	100 μl of a mixture of H2O:MeOH (1:4) was added and the sample was sonicated
SP:SAMPLEPREP_SUMMARY            	again for a total of 15 min at 15W (3 rounds of 5 min sonication), thoroughly
SP:SAMPLEPREP_SUMMARY            	vortexed and centrifuged for 10 min at 16000 g to remove any debris. The
SP:SAMPLEPREP_SUMMARY            	supernatant was collected. Polar samples were kept at 4 degrees until their
SP:SAMPLEPREP_SUMMARY            	analysis. Extraction solvents were used as a blank and followed the same
SP:SAMPLEPREP_SUMMARY            	procedure as the samples. Quality control (QC) samples were prepared by pooling
SP:SAMPLEPREP_SUMMARY            	equal volumes of samples from the same cell type separately. Additionally, to
SP:SAMPLEPREP_SUMMARY            	select features produced only by cells, a calibration curve was made of Teff or
SP:SAMPLEPREP_SUMMARY            	Treg cells, respectively. Samples were divided into 2 groups depending on the
SP:SAMPLEPREP_SUMMARY            	type of subpopulation they belong to either Teff or Treg, and randomized.
SP:EXTRACT_STORAGE               	-80℃
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_SUMMARY        	For polar metabolites separation, 2 μL of the sample were injected into a
CH:CHROMATOGRAPHY_SUMMARY        	Zorbax Extend C18 (4.6 × 50 mm, 1.8 μm; Agilent, Waldbronn, Germany), with a
CH:CHROMATOGRAPHY_SUMMARY        	guard column Zorbax Extend C18 (3 × 5 mm, 1.8 μm; Agilent), both maintained at
CH:CHROMATOGRAPHY_SUMMARY        	60 °C. The flow rate was set at 0.6 mL/min. The elution gradient involved a
CH:CHROMATOGRAPHY_SUMMARY        	mobile phase consisting of: (A) water containing 0.1% of formic acid and (B)
CH:CHROMATOGRAPHY_SUMMARY        	acetonitrile containing 0.1% of formic acid. The initial conditions were set at
CH:CHROMATOGRAPHY_SUMMARY        	5% phase B for 1 min, which increased linearly to 80% phase B in 7 min. Then in
CH:CHROMATOGRAPHY_SUMMARY        	4.5 min it increased until 100% of phase. Then the equipment returned to the
CH:CHROMATOGRAPHY_SUMMARY        	initial conditions in 0.5 min, which were held for 3 min for column
CH:CHROMATOGRAPHY_SUMMARY        	reconditioning.
CH:CHROMATOGRAPHY_TYPE           	Reversed phase
CH:INSTRUMENT_NAME               	Agilent 1290 Infinity II
CH:COLUMN_NAME                   	Agilent ZORBAX RRHD Extend-C18 (50 x 2.1mm,1.8um)
CH:SOLVENT_A                     	water containing 0.1% of formic acid
CH:SOLVENT_B                     	acetonitrile containing 0.1% of formic acid
CH:FLOW_GRADIENT                 	0-1 min: 5% B; 1-8 min: linear increase until 80% B; 8-12.5 min: linear increase
CH:FLOW_GRADIENT                 	until 100% B. Then the equipment returned to the initial conditions in 0.5 min,
CH:FLOW_GRADIENT                 	which were held for 3 min for column reconditioning.
CH:FLOW_RATE                     	0.6 mL/min
CH:COLUMN_TEMPERATURE            	60 ºC
#ANALYSIS
AN:ANALYSIS_TYPE                 	MS
AN:LABORATORY_NAME               	Centre of Metabolomics and Bioanalysis (CEMBIO)
#MS
MS:INSTRUMENT_NAME               	Agilent 6550 QTOF
MS:INSTRUMENT_TYPE               	QTOF
MS:MS_TYPE                       	ESI
MS:ION_MODE                      	POSITIVE
MS:MS_COMMENTS                   	The Agilent 6550 QTOF mass spectrometer equipped with a dual AJS ESI ion source
MS:MS_COMMENTS                   	was set with the following parameters: The capillary voltage was set at 3000 for
MS:MS_COMMENTS                   	both polarities. The drying gas flow rate was 12 L/min at 250 °C and gas
MS:MS_COMMENTS                   	nebulizer at 52 psi; fragmentor voltage was set at 175 V in ESI+ and 250 in
MS:MS_COMMENTS                   	ESI-; skimmer and octupole radio frequency voltages were set to 65 and 750 V,
MS:MS_COMMENTS                   	respectively. MS spectra were collected in the centroid mode at a scan rate of 3
MS:MS_COMMENTS                   	spectra/s. The MS detection window was performed in a full scan from 100 to 1200
MS:MS_COMMENTS                   	m/z for both modes. Automatic MS recalibration during batch analysis was carried
MS:MS_COMMENTS                   	out by introducing a reference standard into the source via a reference sprayer
MS:MS_COMMENTS                   	valve. Reference masses for ESI+ were purine (m/z = 121.0508) and HP-0921 (m/z =
MS:MS_COMMENTS                   	922.0097).
MS:MS_RESULTS_FILE               	ST003135_AN005145_Results.txt	UNITS:Peak area	Has m/z:Yes	Has RT:Yes	RT units:Minutes
#END