#METABOLOMICS WORKBENCH NuriaCG_20240222_040502 DATATRACK_ID:4668 STUDY_ID:ST003135 ANALYSIS_ID:AN005148 PROJECT_ID:PR001948
VERSION             	1
CREATED_ON             	February 29, 2024, 2:04 am
#PROJECT
PR:PROJECT_TITLE                 	Metabolomic study of T effector and T regulatory cells in severe allergic
PR:PROJECT_TITLE                 	patients
PR:PROJECT_TYPE                  	MS untargeted analysis combining lipidomics and metabolomics.
PR:PROJECT_SUMMARY               	Metabolism has a profound impact on T cell fate and function. Uncovering the
PR:PROJECT_SUMMARY               	metabolome of circulating human CD4+ T effector memory (Teff) and T regulatory
PR:PROJECT_SUMMARY               	(Treg) cells would enable better understanding of Th2-driven diseases, such as
PR:PROJECT_SUMMARY               	allergy or asthma. Here, we demonstrated that in healthy humans, energy
PR:PROJECT_SUMMARY               	metabolism and functions of memory CD4+ Teff cells mainly relied on amino acids,
PR:PROJECT_SUMMARY               	whereas Treg cells predominantly used fatty acids. Arginine and phenylalanine
PR:PROJECT_SUMMARY               	increased T cell receptorinduced glycolysis and oxidative phosphorylation in
PR:PROJECT_SUMMARY               	total and memory CD4+ T cells, but high levels of phenylalanine limited CD4+ T
PR:PROJECT_SUMMARY               	cell proliferation via disrupting mitochondrial respiration and activation of
PR:PROJECT_SUMMARY               	L-phenylalanine oxidase, IL4I1. Accordingly, lowest levels of phenylalanine were
PR:PROJECT_SUMMARY               	linked with the pathogenic Th2a cells, and impaired Treg cells in patients with
PR:PROJECT_SUMMARY               	the most severe forms of allergies. It all suggests that phenylalanine is a
PR:PROJECT_SUMMARY               	metabolic checkpoint of pathogenic Th2 cells development.
PR:INSTITUTE                     	Universidad San Pablo-CEU, CEU Universities
PR:DEPARTMENT                    	Química y Bioquímica
PR:LAST_NAME                     	Villaseñor
PR:FIRST_NAME                    	Alma
PR:ADDRESS                       	Urbanización Montepríncipe, n/s, Boadilla del Monte, Madrid, 28003, Spain
PR:EMAIL                         	alma.villasenor@ceu.es
PR:PHONE                         	913724750
#STUDY
ST:STUDY_TITLE                   	Metabolomic study of T effector and T regulatory cells in severe allergic
ST:STUDY_TITLE                   	patients
ST:STUDY_SUMMARY                 	Metabolism has a profound impact on T cell fate and function. Uncovering the
ST:STUDY_SUMMARY                 	metabolome of circulating human CD4+ T effector memory (Teff) and T regulatory
ST:STUDY_SUMMARY                 	(Treg) cells would enable better understanding of Th2-driven diseases, such as
ST:STUDY_SUMMARY                 	allergy or asthma. Here, we demonstrated that in healthy humans, energy
ST:STUDY_SUMMARY                 	metabolism and functions of memory CD4+ Teff cells mainly relied on amino acids,
ST:STUDY_SUMMARY                 	whereas Treg cells predominantly used fatty acids. Arginine and phenylalanine
ST:STUDY_SUMMARY                 	increased T cell receptorinduced glycolysis and oxidative phosphorylation in
ST:STUDY_SUMMARY                 	total and memory CD4+ T cells, but high levels of phenylalanine limited CD4+ T
ST:STUDY_SUMMARY                 	cell proliferation via disrupting mitochondrial respiration and activation of
ST:STUDY_SUMMARY                 	L-phenylalanine oxidase, IL4I1. Accordingly, lowest levels of phenylalanine were
ST:STUDY_SUMMARY                 	linked with the pathogenic Th2a cells, and impaired Treg cells in patients with
ST:STUDY_SUMMARY                 	the most severe forms of allergies. It all suggests that phenylalanine is a
ST:STUDY_SUMMARY                 	metabolic checkpoint of pathogenic Th2 cells development.
ST:INSTITUTE                     	Universidad San Pablo-CEU, CEU Universities
ST:DEPARTMENT                    	Química y Bioquímica
ST:LAST_NAME                     	Villaseñor
ST:FIRST_NAME                    	Alma
ST:ADDRESS                       	Urbanización Montepríncipe, n/s, Boadilla del Monte, Madrid, 28003, Spain
ST:EMAIL                         	alma.villasenor@ceu.es
ST:PHONE                         	913724750
#SUBJECT
SU:SUBJECT_TYPE                  	Human
SU:SUBJECT_SPECIES               	Homo sapiens
SU:TAXONOMY_ID                   	9606
SU:AGE_OR_AGE_RANGE              	Adult patients (>18 years old)
SU:GENDER                        	Male and female
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data
SUBJECT_SAMPLE_FACTORS           	A2	A2_Eff_Polar	Sample source:PBMCs | Experimental_Group:Non_Allergic | Cell_Type:Teff | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=A2_Eff_Polar_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A2_Eff_Polar_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A2_Eff_Polar_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A2_Eff_Polar_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	A2	A2_Reg_Polar	Sample source:PBMCs | Experimental_Group:Non_Allergic | Cell_Type:Treg | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=A2_Reg_Polar_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A2_Reg_Polar_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A2_Reg_Polar_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A2_Reg_Polar_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	A2	A2_Eff_Lipids	Sample source:PBMCs | Experimental_Group:Non_Allergic | Cell_Type:Teff | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=A2_Eff_Lipids_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A2_Eff_Lipids_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A2_Eff_Lipids_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A2_Eff_Lipids_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	A2	A2_Reg_Lipids	Sample source:PBMCs | Experimental_Group:Non_Allergic | Cell_Type:Treg | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=A2_Reg_Lipids_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A2_Reg_Lipids_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A2_Reg_Lipids_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A2_Reg_Lipids_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	A4	A4_Eff_Polar	Sample source:PBMCs | Experimental_Group:Non_Allergic | Cell_Type:Teff | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=A4_Eff_Polar_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A4_Eff_Polar_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A4_Eff_Polar_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A4_Eff_Polar_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	A4	A4_Reg_Polar	Sample source:PBMCs | Experimental_Group:Non_Allergic | Cell_Type:Treg | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=A4_Reg_Polar_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A4_Reg_Polar_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A4_Reg_Polar_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A4_Reg_Polar_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	A4	A4_Eff_Lipids	Sample source:PBMCs | Experimental_Group:Non_Allergic | Cell_Type:Teff | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=A4_Eff_Lipids_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A4_Eff_Lipids_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A4_Eff_Lipids_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A4_Eff_Lipids_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	A4	A4_Reg_Lipids	Sample source:PBMCs | Experimental_Group:Non_Allergic | Cell_Type:Treg | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=A4_Reg_Lipids_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A4_Reg_Lipids_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A4_Reg_Lipids_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A4_Reg_Lipids_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	A5	A5_Eff_Polar	Sample source:PBMCs | Experimental_Group:Non_Allergic | Cell_Type:Teff | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=A5_Eff_Polar_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A5_Eff_Polar_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A5_Eff_Polar_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A5_Eff_Polar_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	A5	A5_Reg_Polar	Sample source:PBMCs | Experimental_Group:Non_Allergic | Cell_Type:Treg | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=A5_Reg_Polar_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A5_Reg_Polar_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A5_Reg_Polar_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A5_Reg_Polar_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	A5	A5_Eff_Lipids	Sample source:PBMCs | Experimental_Group:Non_Allergic | Cell_Type:Teff | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=A5_Eff_Lipids_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A5_Eff_Lipids_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A5_Eff_Lipids_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A5_Eff_Lipids_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	A5	A5_Reg_Lipids	Sample source:PBMCs | Experimental_Group:Non_Allergic | Cell_Type:Treg | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=A5_Reg_Lipids_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A5_Reg_Lipids_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A5_Reg_Lipids_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A5_Reg_Lipids_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	A6	A6_Eff_Polar	Sample source:PBMCs | Experimental_Group:Non_Allergic | Cell_Type:Teff | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=A6_Eff_Polar_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A6_Eff_Polar_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A6_Eff_Polar_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A6_Eff_Polar_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	A6	A6_Reg_Polar	Sample source:PBMCs | Experimental_Group:Non_Allergic | Cell_Type:Treg | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=A6_Reg_Polar_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A6_Reg_Polar_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A6_Reg_Polar_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A6_Reg_Polar_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	A6	A6_Eff_Lipids	Sample source:PBMCs | Experimental_Group:Non_Allergic | Cell_Type:Teff | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=A6_Eff_Lipids_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A6_Eff_Lipids_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A6_Eff_Lipids_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A6_Eff_Lipids_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	A6	A6_Reg_Lipids	Sample source:PBMCs | Experimental_Group:Non_Allergic | Cell_Type:Treg | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=A6_Reg_Lipids_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A6_Reg_Lipids_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A6_Reg_Lipids_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A6_Reg_Lipids_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	A7	A7_Eff_Polar	Sample source:PBMCs | Experimental_Group:Non_Allergic | Cell_Type:Teff | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=A7_Eff_Polar_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A7_Eff_Polar_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A7_Eff_Polar_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A7_Eff_Polar_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	A7	A7_Reg_Polar	Sample source:PBMCs | Experimental_Group:Non_Allergic | Cell_Type:Treg | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=A7_Reg_Polar_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A7_Reg_Polar_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A7_Reg_Polar_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A7_Reg_Polar_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	A7	A7_Eff_Lipids	Sample source:PBMCs | Experimental_Group:Non_Allergic | Cell_Type:Teff | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=A7_Eff_Lipids_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A7_Eff_Lipids_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A7_Eff_Lipids_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A7_Eff_Lipids_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	A7	A7_Reg_Lipids	Sample source:PBMCs | Experimental_Group:Non_Allergic | Cell_Type:Treg | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=A7_Reg_Lipids_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A7_Reg_Lipids_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A7_Reg_Lipids_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A7_Reg_Lipids_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	A8	A8_Eff_Polar	Sample source:PBMCs | Experimental_Group:Non_Allergic | Cell_Type:Teff | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=A8_Eff_Polar_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A8_Eff_Polar_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A8_Eff_Polar_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A8_Eff_Polar_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	A8	A8_Reg_Polar	Sample source:PBMCs | Experimental_Group:Non_Allergic | Cell_Type:Treg | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=A8_Reg_Polar_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A8_Reg_Polar_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A8_Reg_Polar_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A8_Reg_Polar_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	A8	A8_Eff_Lipids	Sample source:PBMCs | Experimental_Group:Non_Allergic | Cell_Type:Teff | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=A8_Eff_Lipids_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A8_Eff_Lipids_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A8_Eff_Lipids_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A8_Eff_Lipids_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	A8	A8_Reg_Lipids	Sample source:PBMCs | Experimental_Group:Non_Allergic | Cell_Type:Treg | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=A8_Reg_Lipids_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A8_Reg_Lipids_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A8_Reg_Lipids_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=A8_Reg_Lipids_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	B2	B2_Eff_Polar	Sample source:PBMCs | Experimental_Group:Mild | Cell_Type:Teff | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=B2_Eff_Polar_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=B2_Eff_Polar_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=B2_Eff_Polar_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=B2_Eff_Polar_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	B2	B2_Reg_Polar	Sample source:PBMCs | Experimental_Group:Mild | Cell_Type:Treg | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=B2_Reg_Polar_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=B2_Reg_Polar_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=B2_Reg_Polar_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=B2_Reg_Polar_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	B2	B2_Eff_Lipids	Sample source:PBMCs | Experimental_Group:Mild | Cell_Type:Teff | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=B2_Eff_Lipids_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=B2_Eff_Lipids_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=B2_Eff_Lipids_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=B2_Eff_Lipids_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	B2	B2_Reg_Lipids	Sample source:PBMCs | Experimental_Group:Mild | Cell_Type:Treg | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=B2_Reg_Lipids_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=B2_Reg_Lipids_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=B2_Reg_Lipids_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=B2_Reg_Lipids_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	B3	B3_Eff_Polar	Sample source:PBMCs | Experimental_Group:Mild | Cell_Type:Teff | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=B3_Eff_Polar_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=B3_Eff_Polar_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=B3_Eff_Polar_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=B3_Eff_Polar_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	B3	B3_Reg_Polar	Sample source:PBMCs | Experimental_Group:Mild | Cell_Type:Treg | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=B3_Reg_Polar_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=B3_Reg_Polar_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=B3_Reg_Polar_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=B3_Reg_Polar_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	B3	B3_Eff_Lipids	Sample source:PBMCs | Experimental_Group:Mild | Cell_Type:Teff | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=B3_Eff_Lipids_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=B3_Eff_Lipids_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=B3_Eff_Lipids_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=B3_Eff_Lipids_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	B3	B3_Reg_Lipids	Sample source:PBMCs | Experimental_Group:Mild | Cell_Type:Treg | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=B3_Reg_Lipids_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=B3_Reg_Lipids_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=B3_Reg_Lipids_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=B3_Reg_Lipids_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	B4	B4_Eff_Polar	Sample source:PBMCs | Experimental_Group:Mild | Cell_Type:Teff | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=B4_Eff_Polar_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=B4_Eff_Polar_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=B4_Eff_Polar_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=B4_Eff_Polar_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	B4	B4_Reg_Polar	Sample source:PBMCs | Experimental_Group:Mild | Cell_Type:Treg | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=B4_Reg_Polar_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=B4_Reg_Polar_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=B4_Reg_Polar_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=B4_Reg_Polar_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	B4	B4_Eff_Lipids	Sample source:PBMCs | Experimental_Group:Mild | Cell_Type:Teff | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=B4_Eff_Lipids_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=B4_Eff_Lipids_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=B4_Eff_Lipids_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=B4_Eff_Lipids_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	B4	B4_Reg_Lipids	Sample source:PBMCs | Experimental_Group:Mild | Cell_Type:Treg | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=B4_Reg_Lipids_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=B4_Reg_Lipids_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=B4_Reg_Lipids_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=B4_Reg_Lipids_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	C1	C1_Eff_Polar	Sample source:PBMCs | Experimental_Group:Severe | Cell_Type:Teff | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=C1_Eff_Polar_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C1_Eff_Polar_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C1_Eff_Polar_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C1_Eff_Polar_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	C1	C1_Reg_Polar	Sample source:PBMCs | Experimental_Group:Severe | Cell_Type:Treg | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=C1_Reg_Polar_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C1_Reg_Polar_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C1_Reg_Polar_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C1_Reg_Polar_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	C1	C1_Eff_Lipids	Sample source:PBMCs | Experimental_Group:Severe | Cell_Type:Teff | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=C1_Eff_Lipids_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C1_Eff_Lipids_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C1_Eff_Lipids_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C1_Eff_Lipids_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	C1	C1_Reg_Lipids	Sample source:PBMCs | Experimental_Group:Severe | Cell_Type:Treg | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=C1_Reg_Lipids_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C1_Reg_Lipids_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C1_Reg_Lipids_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C1_Reg_Lipids_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	C2	C2_Eff_Polar	Sample source:PBMCs | Experimental_Group:Severe | Cell_Type:Teff | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=C2_Eff_Polar_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C2_Eff_Polar_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C2_Eff_Polar_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C2_Eff_Polar_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	C2	C2_Reg_Polar	Sample source:PBMCs | Experimental_Group:Severe | Cell_Type:Treg | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=C2_Reg_Polar_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C2_Reg_Polar_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C2_Reg_Polar_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C2_Reg_Polar_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	C2	C2_Eff_Lipids	Sample source:PBMCs | Experimental_Group:Severe | Cell_Type:Teff | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=C2_Eff_Lipids_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C2_Eff_Lipids_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C2_Eff_Lipids_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C2_Eff_Lipids_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	C2	C2_Reg_Lipids	Sample source:PBMCs | Experimental_Group:Severe | Cell_Type:Treg | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=C2_Reg_Lipids_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C2_Reg_Lipids_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C2_Reg_Lipids_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C2_Reg_Lipids_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	C6	C6_Eff_Polar	Sample source:PBMCs | Experimental_Group:Severe | Cell_Type:Teff | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=C6_Eff_Polar_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C6_Eff_Polar_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C6_Eff_Polar_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C6_Eff_Polar_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	C6	C6_Reg_Polar	Sample source:PBMCs | Experimental_Group:Severe | Cell_Type:Treg | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=C6_Reg_Polar_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C6_Reg_Polar_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C6_Reg_Polar_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C6_Reg_Polar_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	C6	C6_Eff_Lipids	Sample source:PBMCs | Experimental_Group:Severe | Cell_Type:Teff | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=C6_Eff_Lipids_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C6_Eff_Lipids_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C6_Eff_Lipids_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C6_Eff_Lipids_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	C6	C6_Reg_Lipids	Sample source:PBMCs | Experimental_Group:Severe | Cell_Type:Treg | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=C6_Reg_Lipids_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C6_Reg_Lipids_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C6_Reg_Lipids_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C6_Reg_Lipids_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	C7	C7_Eff_Polar	Sample source:PBMCs | Experimental_Group:Severe | Cell_Type:Teff | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=C7_Eff_Polar_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C7_Eff_Polar_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C7_Eff_Polar_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C7_Eff_Polar_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	C7	C7_Reg_Polar	Sample source:PBMCs | Experimental_Group:Severe | Cell_Type:Treg | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=C7_Reg_Polar_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C7_Reg_Polar_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C7_Reg_Polar_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C7_Reg_Polar_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	C7	C7_Eff_Lipids	Sample source:PBMCs | Experimental_Group:Severe | Cell_Type:Teff | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=C7_Eff_Lipids_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C7_Eff_Lipids_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C7_Eff_Lipids_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C7_Eff_Lipids_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	C7	C7_Reg_Lipids	Sample source:PBMCs | Experimental_Group:Severe | Cell_Type:Treg | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=C7_Reg_Lipids_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C7_Reg_Lipids_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C7_Reg_Lipids_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C7_Reg_Lipids_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	C8	C8_Eff_Polar	Sample source:PBMCs | Experimental_Group:Severe | Cell_Type:Teff | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=C8_Eff_Polar_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C8_Eff_Polar_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C8_Eff_Polar_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C8_Eff_Polar_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	C8	C8_Reg_Polar	Sample source:PBMCs | Experimental_Group:Severe | Cell_Type:Treg | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=C8_Reg_Polar_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C8_Reg_Polar_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C8_Reg_Polar_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C8_Reg_Polar_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	C8	C8_Eff_Lipids	Sample source:PBMCs | Experimental_Group:Severe | Cell_Type:Teff | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=C8_Eff_Lipids_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C8_Eff_Lipids_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C8_Eff_Lipids_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C8_Eff_Lipids_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	C8	C8_Reg_Lipids	Sample source:PBMCs | Experimental_Group:Severe | Cell_Type:Treg | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=C8_Reg_Lipids_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C8_Reg_Lipids_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C8_Reg_Lipids_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C8_Reg_Lipids_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	C9	C9_Eff_Polar	Sample source:PBMCs | Experimental_Group:Severe | Cell_Type:Teff | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=C9_Eff_Polar_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C9_Eff_Polar_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C9_Eff_Polar_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C9_Eff_Polar_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	C9	C9_Reg_Polar	Sample source:PBMCs | Experimental_Group:Severe | Cell_Type:Treg | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=C9_Reg_Polar_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C9_Reg_Polar_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C9_Reg_Polar_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C9_Reg_Polar_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	C9	C9_Eff_Lipids	Sample source:PBMCs | Experimental_Group:Severe | Cell_Type:Teff | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=C9_Eff_Lipids_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C9_Eff_Lipids_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C9_Eff_Lipids_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C9_Eff_Lipids_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	C9	C9_Reg_Lipids	Sample source:PBMCs | Experimental_Group:Severe | Cell_Type:Treg | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=C9_Reg_Lipids_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C9_Reg_Lipids_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C9_Reg_Lipids_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C9_Reg_Lipids_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	C10	C10_Eff_Polar	Sample source:PBMCs | Experimental_Group:Severe | Cell_Type:Teff | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=C10_Eff_Polar_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C10_Eff_Polar_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C10_Eff_Polar_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C10_Eff_Polar_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	C10	C10_Reg_Polar	Sample source:PBMCs | Experimental_Group:Severe | Cell_Type:Treg | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=C10_Reg_Polar_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C10_Reg_Polar_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C10_Reg_Polar_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C10_Reg_Polar_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	C10	C10_Eff_Lipids	Sample source:PBMCs | Experimental_Group:Severe | Cell_Type:Teff | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=C10_Eff_Lipids_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C10_Eff_Lipids_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C10_Eff_Lipids_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C10_Eff_Lipids_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	C10	C10_Reg_Lipids	Sample source:PBMCs | Experimental_Group:Severe | Cell_Type:Treg | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=C10_Reg_Lipids_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C10_Reg_Lipids_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C10_Reg_Lipids_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C10_Reg_Lipids_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	C11	C11_Eff_Polar	Sample source:PBMCs | Experimental_Group:Severe | Cell_Type:Teff | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=C11_Eff_Polar_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C11_Eff_Polar_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C11_Eff_Polar_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C11_Eff_Polar_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	C11	C11_Reg_Polar	Sample source:PBMCs | Experimental_Group:Severe | Cell_Type:Treg | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=C11_Reg_Polar_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C11_Reg_Polar_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C11_Reg_Polar_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C11_Reg_Polar_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	C11	C11_Eff_Lipids	Sample source:PBMCs | Experimental_Group:Severe | Cell_Type:Teff | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=C11_Eff_Lipids_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C11_Eff_Lipids_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C11_Eff_Lipids_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C11_Eff_Lipids_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	C11	C11_Reg_Lipids	Sample source:PBMCs | Experimental_Group:Severe | Cell_Type:Treg | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=C11_Reg_Lipids_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C11_Reg_Lipids_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C11_Reg_Lipids_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C11_Reg_Lipids_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	C12	C12_Eff_Polar	Sample source:PBMCs | Experimental_Group:Severe | Cell_Type:Teff | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=C12_Eff_Polar_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C12_Eff_Polar_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C12_Eff_Polar_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C12_Eff_Polar_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	C12	C12_Reg_Polar	Sample source:PBMCs | Experimental_Group:Severe | Cell_Type:Treg | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=C12_Reg_Polar_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C12_Reg_Polar_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C12_Reg_Polar_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C12_Reg_Polar_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	C12	C12_Eff_Lipids	Sample source:PBMCs | Experimental_Group:Severe | Cell_Type:Teff | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=C12_Eff_Lipids_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C12_Eff_Lipids_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C12_Eff_Lipids_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C12_Eff_Lipids_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	C12	C12_Reg_Lipids	Sample source:PBMCs | Experimental_Group:Severe | Cell_Type:Treg | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=C12_Reg_Lipids_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C12_Reg_Lipids_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C12_Reg_Lipids_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C12_Reg_Lipids_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	C13	C13_Eff_Polar	Sample source:PBMCs | Experimental_Group:Severe | Cell_Type:Teff | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=C13_Eff_Polar_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C13_Eff_Polar_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C13_Eff_Polar_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C13_Eff_Polar_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	C13	C13_Reg_Polar	Sample source:PBMCs | Experimental_Group:Severe | Cell_Type:Treg | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=C13_Reg_Polar_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C13_Reg_Polar_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C13_Reg_Polar_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C13_Reg_Polar_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	C13	C13_Eff_Lipids	Sample source:PBMCs | Experimental_Group:Severe | Cell_Type:Teff | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=C13_Eff_Lipids_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C13_Eff_Lipids_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C13_Eff_Lipids_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C13_Eff_Lipids_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	C13	C13_Reg_Lipids	Sample source:PBMCs | Experimental_Group:Severe | Cell_Type:Treg | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=C13_Reg_Lipids_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C13_Reg_Lipids_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C13_Reg_Lipids_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=C13_Reg_Lipids_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	QC1	QC1_Reg_Polar	Sample source:PBMCs | Experimental_Group:Quality_Control | Cell_Type:Treg | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=QC1_Reg_Polar_Pos.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=QC1_Reg_Polar_Neg.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	QC1	QC1_Reg_Lipids	Sample source:PBMCs | Experimental_Group:Quality_Control | Cell_Type:Treg | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=QC1_Reg_Lipids_Pos.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=QC1_Reg_Lipids_Neg.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	QC2	QC2_Reg_Polar	Sample source:PBMCs | Experimental_Group:Quality_Control | Cell_Type:Treg | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=QC2_Reg_Polar_Pos.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=QC2_Reg_Polar_Neg.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	QC2	QC2_Reg_Lipids	Sample source:PBMCs | Experimental_Group:Quality_Control | Cell_Type:Treg | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=QC2_Reg_Lipids_Pos.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=QC2_Reg_Lipids_Neg.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	QC3	QC3_Reg_Polar	Sample source:PBMCs | Experimental_Group:Quality_Control | Cell_Type:Treg | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=QC3_Reg_Polar_Pos.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=QC3_Reg_Polar_Neg.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	QC3	QC3_Reg_Lipids	Sample source:PBMCs | Experimental_Group:Quality_Control | Cell_Type:Treg | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=QC3_Reg_Lipids_Pos.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=QC3_Reg_Lipids_Neg.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	QC4	QC4_Reg_Polar	Sample source:PBMCs | Experimental_Group:Quality_Control | Cell_Type:Treg | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=QC4_Reg_Polar_Pos.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=QC4_Reg_Polar_Neg.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	QC4	QC4_Reg_Lipids	Sample source:PBMCs | Experimental_Group:Quality_Control | Cell_Type:Treg | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=QC4_Reg_Lipids_Pos.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=QC4_Reg_Lipids_Neg.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	QC5	QC5_Reg_Polar	Sample source:PBMCs | Experimental_Group:Quality_Control | Cell_Type:Treg | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=QC5_Reg_Polar_Pos.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=QC5_Reg_Polar_Neg.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	QC5	QC5_Reg_Lipids	Sample source:PBMCs | Experimental_Group:Quality_Control | Cell_Type:Treg | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=QC5_Reg_Lipids_Pos.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=QC5_Reg_Lipids_Neg.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	QC6	QC6_Reg_Polar	Sample source:PBMCs | Experimental_Group:Quality_Control | Cell_Type:Treg | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=QC6_Reg_Polar_Pos.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=QC6_Reg_Polar_Neg.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	QC6	QC6_Reg_Lipids	Sample source:PBMCs | Experimental_Group:Quality_Control | Cell_Type:Treg | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=QC6_Reg_Lipids_Pos.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=QC6_Reg_Lipids_Neg.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	QC7	QC7_Reg_Polar	Sample source:PBMCs | Experimental_Group:Quality_Control | Cell_Type:Treg | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=QC7_Reg_Polar_Pos.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=QC7_Reg_Polar_Neg.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	QC7	QC7_Reg_Lipids	Sample source:PBMCs | Experimental_Group:Quality_Control | Cell_Type:Treg | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=QC7_Reg_Lipids_Pos.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=QC7_Reg_Lipids_Neg.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	QC8	QC8_Reg_Polar	Sample source:PBMCs | Experimental_Group:Quality_Control | Cell_Type:Treg | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=QC8_Reg_Polar_Pos.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=QC8_Reg_Polar_Neg.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	QC8	QC8_Reg_Lipids	Sample source:PBMCs | Experimental_Group:Quality_Control | Cell_Type:Treg | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=QC8_Reg_Lipids_Pos.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=QC8_Reg_Lipids_Neg.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	QC9	QC9_Reg_Polar	Sample source:PBMCs | Experimental_Group:Quality_Control | Cell_Type:Treg | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=QC9_Reg_Polar_Pos.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=QC9_Reg_Polar_Neg.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	QC9	QC9_Reg_Lipids	Sample source:PBMCs | Experimental_Group:Quality_Control | Cell_Type:Treg | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=QC9_Reg_Lipids_Pos.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=QC9_Reg_Lipids_Neg.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	QC10	QC10_Reg_Polar	Sample source:PBMCs | Experimental_Group:Quality_Control | Cell_Type:Treg | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=QC10_Reg_Polar_Pos.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=QC10_Reg_Polar_Neg.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	QC10	QC10_Reg_Lipids	Sample source:PBMCs | Experimental_Group:Quality_Control | Cell_Type:Treg | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=QC10_Reg_Lipids_Pos.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=QC10_Reg_Lipids_Neg.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	QC12	QC12_Eff_Polar	Sample source:PBMCs | Experimental_Group:Quality_Control | Cell_Type:Teff | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=QC12_Eff_Polar_Pos.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=QC12_Eff_Polar_Neg.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	QC12	QC12_Eff_Lipids	Sample source:PBMCs | Experimental_Group:Quality_Control | Cell_Type:Teff | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=QC12_Eff_Lipids_Pos.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=QC12_Eff_Lipids_Neg.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	QC13	QC13_Eff_Polar	Sample source:PBMCs | Experimental_Group:Quality_Control | Cell_Type:Teff | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=QC13_Eff_Polar_Pos.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=QC13_Eff_Polar_Neg.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	QC13	QC13_Eff_Lipids	Sample source:PBMCs | Experimental_Group:Quality_Control | Cell_Type:Teff | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=QC13_Eff_Lipids_Pos.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=QC13_Eff_Lipids_Neg.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	QC14	QC14_Eff_Polar	Sample source:PBMCs | Experimental_Group:Quality_Control | Cell_Type:Teff | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=QC14_Eff_Polar_Pos.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=QC14_Eff_Polar_Neg.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	QC14	QC14_Eff_Lipids	Sample source:PBMCs | Experimental_Group:Quality_Control | Cell_Type:Teff | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=QC14_Eff_Lipids_Pos.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=QC14_Eff_Lipids_Neg.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	QC15	QC15_Eff_Polar	Sample source:PBMCs | Experimental_Group:Quality_Control | Cell_Type:Teff | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=QC15_Eff_Polar_Pos.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=QC15_Eff_Polar_Neg.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	QC15	QC15_Eff_Lipids	Sample source:PBMCs | Experimental_Group:Quality_Control | Cell_Type:Teff | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=QC15_Eff_Lipids_Pos.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=QC15_Eff_Lipids_Neg.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	QC16	QC16_Eff_Polar	Sample source:PBMCs | Experimental_Group:Quality_Control | Cell_Type:Teff | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=QC16_Eff_Polar_Pos.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=QC16_Eff_Polar_Neg.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	QC16	QC16_Eff_Lipids	Sample source:PBMCs | Experimental_Group:Quality_Control | Cell_Type:Teff | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=QC16_Eff_Lipids_Pos.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=QC16_Eff_Lipids_Neg.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	QC17	QC17_Eff_Polar	Sample source:PBMCs | Experimental_Group:Quality_Control | Cell_Type:Teff | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=QC17_Eff_Polar_Pos.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=QC17_Eff_Polar_Neg.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	QC17	QC17_Eff_Lipids	Sample source:PBMCs | Experimental_Group:Quality_Control | Cell_Type:Teff | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=QC17_Eff_Lipids_Pos.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=QC17_Eff_Lipids_Neg.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	QC18	QC18_Eff_Polar	Sample source:PBMCs | Experimental_Group:Quality_Control | Cell_Type:Teff | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=QC18_Eff_Polar_Pos.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=QC18_Eff_Polar_Neg.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	QC18	QC18_Eff_Lipids	Sample source:PBMCs | Experimental_Group:Quality_Control | Cell_Type:Teff | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=QC18_Eff_Lipids_Pos.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=QC18_Eff_Lipids_Neg.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	QC19	QC19_Eff_Polar	Sample source:PBMCs | Experimental_Group:Quality_Control | Cell_Type:Teff | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=QC19_Eff_Polar_Pos.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=QC19_Eff_Polar_Neg.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	QC19	QC19_Eff_Lipids	Sample source:PBMCs | Experimental_Group:Quality_Control | Cell_Type:Teff | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=QC19_Eff_Lipids_Pos.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=QC19_Eff_Lipids_Neg.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	QC20	QC20_Eff_Polar	Sample source:PBMCs | Experimental_Group:Quality_Control | Cell_Type:Teff | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=QC20_Eff_Polar_Pos.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=QC20_Eff_Polar_Neg.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	QC20	QC20_Eff_Lipids	Sample source:PBMCs | Experimental_Group:Quality_Control | Cell_Type:Teff | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=QC20_Eff_Lipids_Pos.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=QC20_Eff_Lipids_Neg.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	QC21	QC21_Eff_Polar	Sample source:PBMCs | Experimental_Group:Quality_Control | Cell_Type:Teff | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=QC21_Eff_Polar_Pos.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=QC21_Eff_Polar_Neg.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	QC21	QC21_Eff_Lipids	Sample source:PBMCs | Experimental_Group:Quality_Control | Cell_Type:Teff | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=QC21_Eff_Lipids_Pos.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=QC21_Eff_Lipids_Neg.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA; RAW_FILE_NAME(RAW_FILE_NAME)=NA
SUBJECT_SAMPLE_FACTORS           	Curve_10	Curve_10_Reg_Polar	Sample source:PBMCs | Experimental_Group:Cell_Curve | Cell_Type:Treg | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=Curve_10_Reg_Polar_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_10_Reg_Polar_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_10_Reg_Polar_Pos_r3.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_10_Reg_Polar_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_10_Reg_Polar_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_10_Reg_Polar_Neg_r3.mzML
SUBJECT_SAMPLE_FACTORS           	Curve_10	Curve_10_Reg_Lipids	Sample source:PBMCs | Experimental_Group:Cell_Curve | Cell_Type:Treg | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=Curve_10_Reg_Lipids_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_10_Reg_Lipids_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_10_Reg_Lipids_Pos_r3.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_10_Reg_Lipids_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_10_Reg_Lipids_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_10_Reg_Lipids_Neg_r3.mzML
SUBJECT_SAMPLE_FACTORS           	Curve_25	Curve_25_Reg_Polar	Sample source:PBMCs | Experimental_Group:Cell_Curve | Cell_Type:Treg | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=Curve_25_Reg_Polar_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_25_Reg_Polar_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_25_Reg_Polar_Pos_r3.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_25_Reg_Polar_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_25_Reg_Polar_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_25_Reg_Polar_Neg_r3.mzML
SUBJECT_SAMPLE_FACTORS           	Curve_25	Curve_25_Reg_Lipids	Sample source:PBMCs | Experimental_Group:Cell_Curve | Cell_Type:Treg | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=Curve_25_Reg_Lipids_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_25_Reg_Lipids_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_25_Reg_Lipids_Pos_r3.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_25_Reg_Lipids_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_25_Reg_Lipids_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_25_Reg_Lipids_Neg_r3.mzML
SUBJECT_SAMPLE_FACTORS           	Curve_50	Curve_50_Reg_Polar	Sample source:PBMCs | Experimental_Group:Cell_Curve | Cell_Type:Treg | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=Curve_50_Reg_Polar_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_50_Reg_Polar_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_50_Reg_Polar_Pos_r3.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_50_Reg_Polar_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_50_Reg_Polar_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_50_Reg_Polar_Neg_r3.mzML
SUBJECT_SAMPLE_FACTORS           	Curve_50	Curve_50_Reg_Lipids	Sample source:PBMCs | Experimental_Group:Cell_Curve | Cell_Type:Treg | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=Curve_50_Reg_Lipids_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_50_Reg_Lipids_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_50_Reg_Lipids_Pos_r3.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_50_Reg_Lipids_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_50_Reg_Lipids_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_50_Reg_Lipids_Neg_r3.mzML
SUBJECT_SAMPLE_FACTORS           	Curve_100	Curve_100_Reg_Polar	Sample source:PBMCs | Experimental_Group:Cell_Curve | Cell_Type:Treg | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=Curve_100_Reg_Polar_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_100_Reg_Polar_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_100_Reg_Polar_Pos_r3.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_100_Reg_Polar_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_100_Reg_Polar_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_100_Reg_Polar_Neg_r3.mzML
SUBJECT_SAMPLE_FACTORS           	Curve_100	Curve_100_Reg_Lipids	Sample source:PBMCs | Experimental_Group:Cell_Curve | Cell_Type:Treg | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=Curve_100_Reg_Lipids_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_100_Reg_Lipids_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_100_Reg_Lipids_Pos_r3.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_100_Reg_Lipids_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_100_Reg_Lipids_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_100_Reg_Lipids_Neg_r3.mzML
SUBJECT_SAMPLE_FACTORS           	Curve_100	Curve_100_Eff_Polar	Sample source:PBMCs | Experimental_Group:Cell_Curve | Cell_Type:Teff | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=Curve_100_Eff_Polar_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_100_Eff_Polar_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_100_Eff_Polar_Pos_r3.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_100_Eff_Polar_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_100_Eff_Polar_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_100_Eff_Polar_Neg_r3.mzML
SUBJECT_SAMPLE_FACTORS           	Curve_100	Curve_100_Eff_Lipids	Sample source:PBMCs | Experimental_Group:Cell_Curve | Cell_Type:Teff | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=Curve_100_Eff_Lipids_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_100_Eff_Lipids_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_100_Eff_Lipids_Pos_r3.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_100_Eff_Lipids_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_100_Eff_Lipids_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_100_Eff_Lipids_Neg_r3.mzML
SUBJECT_SAMPLE_FACTORS           	Curve_250	Curve_250_Eff_Polar	Sample source:PBMCs | Experimental_Group:Cell_Curve | Cell_Type:Teff | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=Curve_250_Eff_Polar_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_250_Eff_Polar_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_250_Eff_Polar_Pos_r3.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_250_Eff_Polar_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_250_Eff_Polar_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_250_Eff_Polar_Neg_r3.mzML
SUBJECT_SAMPLE_FACTORS           	Curve_250	Curve_250_Eff_Lipids	Sample source:PBMCs | Experimental_Group:Cell_Curve | Cell_Type:Teff | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=Curve_250_Eff_Lipids_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_250_Eff_Lipids_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_250_Eff_Lipids_Pos_r3.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_250_Eff_Lipids_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_250_Eff_Lipids_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_250_Eff_Lipids_Neg_r3.mzML
SUBJECT_SAMPLE_FACTORS           	Curve_500	Curve_500_Eff_Polar	Sample source:PBMCs | Experimental_Group:Cell_Curve | Cell_Type:Teff | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=Curve_500_Eff_Polar_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_500_Eff_Polar_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_500_Eff_Polar_Pos_r3.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_500_Eff_Polar_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_500_Eff_Polar_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_500_Eff_Polar_Neg_r3.mzML
SUBJECT_SAMPLE_FACTORS           	Curve_500	Curve_500_Eff_Lipids	Sample source:PBMCs | Experimental_Group:Cell_Curve | Cell_Type:Teff | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=Curve_500_Eff_Lipids_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_500_Eff_Lipids_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_500_Eff_Lipids_Pos_r3.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_500_Eff_Lipids_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_500_Eff_Lipids_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_500_Eff_Lipids_Neg_r3.mzML
SUBJECT_SAMPLE_FACTORS           	Curve_1500	Curve_1500_Eff_Polar	Sample source:PBMCs | Experimental_Group:Cell_Curve | Cell_Type:Teff | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=Curve_1500_Eff_Polar_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_1500_Eff_Polar_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_1500_Eff_Polar_Pos_r3.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_1500_Eff_Polar_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_1500_Eff_Polar_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_1500_Eff_Polar_Neg_r3.mzML
SUBJECT_SAMPLE_FACTORS           	Curve_1500	Curve_1500_Eff_Lipids	Sample source:PBMCs | Experimental_Group:Cell_Curve | Cell_Type:Teff | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=Curve_1500_Eff_Lipids_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_1500_Eff_Lipids_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_1500_Eff_Lipids_Pos_r3.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_1500_Eff_Lipids_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_1500_Eff_Lipids_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Curve_1500_Eff_Lipids_Neg_r3.mzML
SUBJECT_SAMPLE_FACTORS           	Blank	Blank_Reg_Polar	Sample source:PBMCs | Experimental_Group:Blank | Cell_Type:Treg | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=Blank_Reg_Polar_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Blank_Reg_Polar_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Blank_Reg_Polar_Pos_r3.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Blank_Reg_Polar_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Blank_Reg_Polar_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Blank_Reg_Polar_Neg_r3.mzML
SUBJECT_SAMPLE_FACTORS           	Blank	Blank_Reg_Lipids	Sample source:PBMCs | Experimental_Group:Blank | Cell_Type:Treg | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=Blank_Reg_Lipids_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Blank_Reg_Lipids_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Blank_Reg_Lipids_Pos_r3.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Blank_Reg_Lipids_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Blank_Reg_Lipids_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Blank_Reg_Lipids_Neg_r3.mzML
SUBJECT_SAMPLE_FACTORS           	Blank	Blank_Eff_Polar	Sample source:PBMCs | Experimental_Group:Blank | Cell_Type:Teff | Extraction_Method:Polar	RAW_FILE_NAME(RAW_FILE_NAME)=Blank_Eff_Polar_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Blank_Eff_Polar_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Blank_Eff_Polar_Pos_r3.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Blank_Eff_Polar_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Blank_Eff_Polar_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Blank_Eff_Polar_Neg_r3.mzML
SUBJECT_SAMPLE_FACTORS           	Blank	Blank_Eff_Lipids	Sample source:PBMCs | Experimental_Group:Blank | Cell_Type:Teff | Extraction_Method:Lipidomics	RAW_FILE_NAME(RAW_FILE_NAME)=Blank_Eff_Lipids_Pos_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Blank_Eff_Lipids_Pos_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Blank_Eff_Lipids_Pos_r3.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Blank_Eff_Lipids_Neg_r1.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Blank_Eff_Lipids_Neg_r2.mzML; RAW_FILE_NAME(RAW_FILE_NAME)=Blank_Eff_Lipids_Neg_r3.mzML
#COLLECTION
CO:COLLECTION_SUMMARY            	Whole blood was drawn from the participants into heparin tubes and serum in
CO:COLLECTION_SUMMARY            	silica tubes. Next, PBMCs were isolated using Ficoll Plaque by gradient density
CO:COLLECTION_SUMMARY            	centrifugation according to manufacturer instructions. Isolated PMBCs were then
CO:COLLECTION_SUMMARY            	immediately frozen and kept at -80˚C until analysis. Teff and Treg cell
CO:COLLECTION_SUMMARY            	subpopulations were determined through FlowJo X (v.10.7.1.) and FlowSOM (v.2.6).
CO:SAMPLE_TYPE                   	Blood (whole)
CO:STORAGE_CONDITIONS            	-80℃
#TREATMENT
TR:TREATMENT_SUMMARY             	After cell sorting, the only treatment received was the addition of a volume of
TR:TREATMENT_SUMMARY             	50 uL of MeOH to each sample and then cells were frozen at -80 ºC until
TR:TREATMENT_SUMMARY             	metabolomics and lipidomic analysis.
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	Frozen cells in MeOH were thawed at 4˚C. Then a first extraction targeted at
SP:SAMPLEPREP_SUMMARY            	lipid metabolites was performed by adding MTBE to a final concentration of MTBE:
SP:SAMPLEPREP_SUMMARY            	MeOH (1:4). Samples were then sonicated for a total of 15 min at 15W (3 rounds
SP:SAMPLEPREP_SUMMARY            	of 5 min sonication), thoroughly vortexed, and centrifuged for 10 min at 16000 g
SP:SAMPLEPREP_SUMMARY            	to remove any debris. The supernatant was collected. Then a second extraction
SP:SAMPLEPREP_SUMMARY            	took place to obtain the most polar metabolites in the sample. To that extent,
SP:SAMPLEPREP_SUMMARY            	100 μl of a mixture of H2O:MeOH (1:4) was added and the sample was sonicated
SP:SAMPLEPREP_SUMMARY            	again for a total of 15 min at 15W (3 rounds of 5 min sonication), thoroughly
SP:SAMPLEPREP_SUMMARY            	vortexed and centrifuged for 10 min at 16000 g to remove any debris. The
SP:SAMPLEPREP_SUMMARY            	supernatant was collected. Polar samples were kept at 4 degrees until their
SP:SAMPLEPREP_SUMMARY            	analysis. Extraction solvents were used as a blank and followed the same
SP:SAMPLEPREP_SUMMARY            	procedure as the samples. Quality control (QC) samples were prepared by pooling
SP:SAMPLEPREP_SUMMARY            	equal volumes of samples from the same cell type separately. Additionally, to
SP:SAMPLEPREP_SUMMARY            	select features produced only by cells, a calibration curve was made of Teff or
SP:SAMPLEPREP_SUMMARY            	Treg cells, respectively. Samples were divided into 2 groups depending on the
SP:SAMPLEPREP_SUMMARY            	type of subpopulation they belong to either Teff or Treg, and randomized.
SP:EXTRACT_STORAGE               	-80℃
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_SUMMARY        	For the lipidic extraction, the Agilent 1290 Infinity II Multisampler system,
CH:CHROMATOGRAPHY_SUMMARY        	equipped with a multi-wash option, was used to uptake 1 and 2 µL of extracted
CH:CHROMATOGRAPHY_SUMMARY        	samples in positive and negative ionization modes, respectively. The
CH:CHROMATOGRAPHY_SUMMARY        	multisampler temperature was maintained at 15 °C to preserve lipids in a stable
CH:CHROMATOGRAPHY_SUMMARY        	environment and avoid precipitation. An Agilent InfinityLab Poroshell 120
CH:CHROMATOGRAPHY_SUMMARY        	ECsingle bondC18 (3.0 × 100 mm, 2.7 µm) (Agilent Technologies) column and a
CH:CHROMATOGRAPHY_SUMMARY        	compatible guard column (Agilent InfinityLab Poroshell 120 ECsingle bondC18, 3.0
CH:CHROMATOGRAPHY_SUMMARY        	× 5 mm, 2.7 µm) were used and maintained at 50 °C. The chromatography
CH:CHROMATOGRAPHY_SUMMARY        	gradient started at 70% of B at 0 – 1 min, 86% at 3.5 – 10 min, 100% B at
CH:CHROMATOGRAPHY_SUMMARY        	11–17 min. The starting conditions were recovered by minute 17, followed by a
CH:CHROMATOGRAPHY_SUMMARY        	2 min re-equilibration time; the total running time was 19 min. The mobile
CH:CHROMATOGRAPHY_SUMMARY        	phases used for both positive and negative ionization modes consisted of (A) 10
CH:CHROMATOGRAPHY_SUMMARY        	mM ammonium acetate, 0.2 mM ammonium fluoride in 9:1 water/methanol and (B) 10
CH:CHROMATOGRAPHY_SUMMARY        	mM ammonium acetate, 0.2 mM ammonium fluoride in 2:3:5
CH:CHROMATOGRAPHY_SUMMARY        	acetonitrile/methanol/isopropanol. The flow rate was held constant, set at 0.6
CH:CHROMATOGRAPHY_SUMMARY        	mL/min. The multi-wash strategy consisted of a mixture of methanol:isopropanol
CH:CHROMATOGRAPHY_SUMMARY        	(50:50, v/v) with the wash time set at 15 s, and aqueous phase:organic phase
CH:CHROMATOGRAPHY_SUMMARY        	(30:70, v/v) mixture to assist in the starting conditions.
CH:CHROMATOGRAPHY_TYPE           	Reversed phase
CH:INSTRUMENT_NAME               	Agilent 1290 Infinity II
CH:COLUMN_NAME                   	Agilent InfinityLab Poroshell 120 EC-C18 (100 x 3mm,2.7um)
CH:SOLVENT_A                     	10 mM ammonium acetate, 0.2 mM ammonium fluoride in 9:1 water/methanol
CH:SOLVENT_B                     	10 mM ammonium acetate, 0.2 mM ammonium fluoride in 2:3:5
CH:SOLVENT_B                     	acetonitrile/methanol/isopropanol
CH:FLOW_GRADIENT                 	0-1 min: 70% B; 1-3.5 min: linear increase until 86% B; 3.5-10 min: 86% B; 10-11
CH:FLOW_GRADIENT                 	min: linear increase until 100% B; 11-17 min: 100% B. Then the equipment
CH:FLOW_GRADIENT                 	returned to the initial conditions in 0.1 min, which were held for 1.9 min for
CH:FLOW_GRADIENT                 	column reconditioning.
CH:FLOW_RATE                     	0.6 mL/min
CH:COLUMN_TEMPERATURE            	50 ºC
#ANALYSIS
AN:ANALYSIS_TYPE                 	MS
AN:LABORATORY_NAME               	Centre of Metabolomics and Bioanalysis (CEMBIO)
#MS
MS:INSTRUMENT_NAME               	Agilent 6550 QTOF
MS:INSTRUMENT_TYPE               	QTOF
MS:MS_TYPE                       	ESI
MS:ION_MODE                      	NEGATIVE
MS:MS_COMMENTS                   	The Agilent 6550 QTOF mass spectrometer equipped with a dual AJS ESI ion source
MS:MS_COMMENTS                   	was set with the following parameters: 150 V fragmentor, 65 V skimmer, 3500 V
MS:MS_COMMENTS                   	capillary voltage, 750 V octopole radio frequency voltage, 10 L/min nebulizer
MS:MS_COMMENTS                   	gas flow, 200 °C gas temperature, 50 psi nebulizer gas pressure, 12 L/min
MS:MS_COMMENTS                   	sheath gas flow, and 300 °C sheath gas temperature. Data were collected in
MS:MS_COMMENTS                   	centroid in mode positive and negative ESI modes in separate runs, operated in
MS:MS_COMMENTS                   	full scan mode from 50 to 1700 m/z with a scan rate of 3 spectra/s. A solution
MS:MS_COMMENTS                   	consisting of two reference mass compounds was used throughout the whole
MS:MS_COMMENTS                   	analysis: purine (C5H4N4) at m/z 119.0363 and HP-0921 (C18H18O6N3P3F24) at m/z
MS:MS_COMMENTS                   	980.0163 (HP-0921+acetate) for the negative ionization modes. These masses were
MS:MS_COMMENTS                   	continuously infused into the system through an Agilent 1260 Iso Pump at a 1
MS:MS_COMMENTS                   	mL/min (split ratio 1:100) to provide a constant mass correction.
MS:MS_RESULTS_FILE               	ST003135_AN005148_Results.txt	UNITS:Peak area	Has m/z:Yes	Has RT:Yes	RT units:Minutes
#END