#METABOLOMICS WORKBENCH iuk41_20240328_121846 DATATRACK_ID:4749 STUDY_ID:ST003149 ANALYSIS_ID:AN005167 PROJECT_ID:PR001958
VERSION                          	1
CREATED_ON                       	08-28-2024
#PROJECT
PR:PROJECT_TITLE                 	Impact of early-life exposure to a potent aryl hydrocarbon receptor ligand on
PR:PROJECT_TITLE                 	gut microbiota and host glucose homeostasis in C57BL/6J male mice
PR:PROJECT_SUMMARY               	This study aimed to explore the association between early-life exposure to a
PR:PROJECT_SUMMARY               	potent aryl hydrocarbon receptor (AHR) agonist and persistent disruptions in the
PR:PROJECT_SUMMARY               	microbiota, leading to impaired metabolic homeostasis later in life. This study
PR:PROJECT_SUMMARY               	utilized metagenomics, NMR- and mass spectrometry-based metabolomics, and
PR:PROJECT_SUMMARY               	biochemical assays to analyze the gut microbiome composition and function, as
PR:PROJECT_SUMMARY               	well as the physiological and metabolic effects of early-life exposure to
PR:PROJECT_SUMMARY               	2,3,7,8-tetrachlorodibenzofuran (TCDF) in conventional, germ-free (GF), and
PR:PROJECT_SUMMARY               	Ahr-null mice. The impact of TCDF on Akkermansia muciniphila (A. muciniphila) in
PR:PROJECT_SUMMARY               	vitro was assessed using optical density (OD 600), flow cytometry,
PR:PROJECT_SUMMARY               	transcriptomics, and mass spectrometry-based metabolomics. TCDF-exposed mice
PR:PROJECT_SUMMARY               	exhibited disruption in the gut microbiome community structure and function,
PR:PROJECT_SUMMARY               	characterized by lower abundances of A. muciniphila, lower levels of cecal short
PR:PROJECT_SUMMARY               	chain fatty acids (SCFAs) and indole-3-lactic acid (ILA), and a reduction in gut
PR:PROJECT_SUMMARY               	hormones GLP-1 and PYY. Importantly, microbial and metabolic phenotypes
PR:PROJECT_SUMMARY               	associated with early-life POP exposure were transferable to GF recipients in
PR:PROJECT_SUMMARY               	the absence of POP carry-over. In addition, AHR-independent interactions between
PR:PROJECT_SUMMARY               	POPs and the microbiota were observed, significantly affected the growth,
PR:PROJECT_SUMMARY               	physiology, gene expression, and metabolic activity of A. muciniphila, resulting
PR:PROJECT_SUMMARY               	in suppressed activity along the ILA pathway.
PR:INSTITUTE                     	Pennsylvania State University
PR:DEPARTMENT                    	Department of Veterinary and Biomedical Sciences
PR:LAST_NAME                     	Koo
PR:FIRST_NAME                    	Imhoi
PR:ADDRESS                       	307B Life Science Building
PR:EMAIL                         	iuk41@psu.edu
PR:PHONE                         	+1 814-865-7803
PR:DOI                           	http://dx.doi.org/10.21228/M8MX5P
#STUDY
ST:STUDY_TITLE                   	Impact of early-life exposure to a potent aryl hydrocarbon receptor ligand on
ST:STUDY_TITLE                   	gut microbiota and host glucose homeostasis in C57BL/6J male mice (Part I)
ST:STUDY_SUMMARY                 	This study aimed to explore the association between early-life exposure to a
ST:STUDY_SUMMARY                 	potent aryl hydrocarbon receptor (AHR) agonist and persistent disruptions in the
ST:STUDY_SUMMARY                 	microbiota, leading to impaired metabolic homeostasis later in life. This study
ST:STUDY_SUMMARY                 	utilized metagenomics, NMR- and mass spectrometry-based metabolomics, and
ST:STUDY_SUMMARY                 	biochemical assays to analyze the gut microbiome composition and function, as
ST:STUDY_SUMMARY                 	well as the physiological and metabolic effects of early-life exposure to
ST:STUDY_SUMMARY                 	2,3,7,8-tetrachlorodibenzofuran (TCDF) in conventional, germ-free (GF), and
ST:STUDY_SUMMARY                 	Ahr-null mice. The impact of TCDF on Akkermansia muciniphila (A. muciniphila) in
ST:STUDY_SUMMARY                 	vitro was assessed using optical density (OD 600), flow cytometry,
ST:STUDY_SUMMARY                 	transcriptomics, and mass spectrometry-based metabolomics. TCDF-exposed mice
ST:STUDY_SUMMARY                 	exhibited disruption in the gut microbiome community structure and function,
ST:STUDY_SUMMARY                 	characterized by lower abundances of A. muciniphila, lower levels of cecal short
ST:STUDY_SUMMARY                 	chain fatty acids (SCFAs) and indole-3-lactic acid (ILA), and a reduction in gut
ST:STUDY_SUMMARY                 	hormones GLP-1 and PYY. Importantly, microbial and metabolic phenotypes
ST:STUDY_SUMMARY                 	associated with early-life POP exposure were transferable to GF recipients in
ST:STUDY_SUMMARY                 	the absence of POP carry-over. In addition, AHR-independent interactions between
ST:STUDY_SUMMARY                 	POPs and the microbiota were observed, significantly affected the growth,
ST:STUDY_SUMMARY                 	physiology, gene expression, and metabolic activity of A. muciniphila, resulting
ST:STUDY_SUMMARY                 	in suppressed activity along the ILA pathway.
ST:INSTITUTE                     	Pennsylvania State University
ST:DEPARTMENT                    	Department of Veterinary and Biomedical Sciences
ST:LAST_NAME                     	Koo
ST:FIRST_NAME                    	Imhoi
ST:ADDRESS                       	307B Life Science Building
ST:EMAIL                         	iuk41@psu.edu
ST:PHONE                         	+1 814-865-7803
ST:SUBMIT_DATE                   	2024-03-28
#SUBJECT
SU:SUBJECT_TYPE                  	Mammal
SU:SUBJECT_SPECIES               	Mus musculus
SU:TAXONOMY_ID                   	10090
SU:AGE_OR_AGE_RANGE              	4 weeks to 5 months
SU:GENDER                        	Male
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data
SUBJECT_SAMPLE_FACTORS           	GF.cecal.content_Akkermansia__10	GF.cecal.content_Akkermansia__10	Sample source:cecal content | Genotype:GF C57BL/6J mice | Treatment:ABX water	RAW_FILE_NAME=2.GF mice cecal content_Akkermansia transplantation.10.fid
SUBJECT_SAMPLE_FACTORS           	GF.cecal.content_Akkermansia__8	GF.cecal.content_Akkermansia__8	Sample source:cecal content | Genotype:GF C57BL/6J mice | Treatment:ABX water	RAW_FILE_NAME=2.GF mice cecal content_Akkermansia transplantation.8.fid
SUBJECT_SAMPLE_FACTORS           	GF.cecal.content_Akkermansia__9	GF.cecal.content_Akkermansia__9	Sample source:cecal content | Genotype:GF C57BL/6J mice | Treatment:ABX water	RAW_FILE_NAME=2.GF mice cecal content_Akkermansia transplantation.9.fid
SUBJECT_SAMPLE_FACTORS           	GF.cecal.content_Akkermansia__1	GF.cecal.content_Akkermansia__1	Sample source:cecal content | Genotype:GF C57BL/6J mice | Treatment:Akkermansia	RAW_FILE_NAME=2.GF mice cecal content_Akkermansia transplantation.1.fid
SUBJECT_SAMPLE_FACTORS           	GF.cecal.content_Akkermansia__2	GF.cecal.content_Akkermansia__2	Sample source:cecal content | Genotype:GF C57BL/6J mice | Treatment:Akkermansia	RAW_FILE_NAME=2.GF mice cecal content_Akkermansia transplantation.2.fid
SUBJECT_SAMPLE_FACTORS           	GF.cecal.content_Akkermansia__3	GF.cecal.content_Akkermansia__3	Sample source:cecal content | Genotype:GF C57BL/6J mice | Treatment:Akkermansia	RAW_FILE_NAME=2.GF mice cecal content_Akkermansia transplantation.3.fid
SUBJECT_SAMPLE_FACTORS           	GF.cecal.content_Akkermansia__4	GF.cecal.content_Akkermansia__4	Sample source:cecal content | Genotype:GF C57BL/6J mice | Treatment:Akkermansia	RAW_FILE_NAME=2.GF mice cecal content_Akkermansia transplantation.4.fid
SUBJECT_SAMPLE_FACTORS           	GF.cecal.content_Akkermansia__5	GF.cecal.content_Akkermansia__5	Sample source:cecal content | Genotype:GF C57BL/6J mice | Treatment:Akkermansia	RAW_FILE_NAME=2.GF mice cecal content_Akkermansia transplantation.5.fid
SUBJECT_SAMPLE_FACTORS           	GF.cecal.content_Akkermansia__11	GF.cecal.content_Akkermansia__11	Sample source:cecal content | Genotype:GF C57BL/6J mice | Treatment:Akkermansia +ABX water	RAW_FILE_NAME=2.GF mice cecal content_Akkermansia transplantation.11.fid
SUBJECT_SAMPLE_FACTORS           	GF.cecal.content_Akkermansia__12	GF.cecal.content_Akkermansia__12	Sample source:cecal content | Genotype:GF C57BL/6J mice | Treatment:Akkermansia +ABX water	RAW_FILE_NAME=2.GF mice cecal content_Akkermansia transplantation.12.fid
SUBJECT_SAMPLE_FACTORS           	GF.cecal.content_Akkermansia__6	GF.cecal.content_Akkermansia__6	Sample source:cecal content | Genotype:GF C57BL/6J mice | Treatment:Akkermansia +ABX water	RAW_FILE_NAME=2.GF mice cecal content_Akkermansia transplantation.6.fid
SUBJECT_SAMPLE_FACTORS           	GF.cecal.content_Akkermansia__7	GF.cecal.content_Akkermansia__7	Sample source:cecal content | Genotype:GF C57BL/6J mice | Treatment:Akkermansia +ABX water	RAW_FILE_NAME=2.GF mice cecal content_Akkermansia transplantation.7.fid
SUBJECT_SAMPLE_FACTORS           	GF.cecal.content_cecal.bacteria__6	GF.cecal.content_cecal.bacteria__6	Sample source:cecal content | Genotype:GF C57BL/6J mice | Treatment:cecal gavage from TCDF group	RAW_FILE_NAME=3.GF mice cecal content_cecal bacteria transplantantion.6.fid
SUBJECT_SAMPLE_FACTORS           	GF.cecal.content_cecal.bacteria__7	GF.cecal.content_cecal.bacteria__7	Sample source:cecal content | Genotype:GF C57BL/6J mice | Treatment:cecal gavage from TCDF group	RAW_FILE_NAME=3.GF mice cecal content_cecal bacteria transplantantion.7.fid
SUBJECT_SAMPLE_FACTORS           	GF.cecal.content_cecal.bacteria__8	GF.cecal.content_cecal.bacteria__8	Sample source:cecal content | Genotype:GF C57BL/6J mice | Treatment:cecal gavage from TCDF group	RAW_FILE_NAME=3.GF mice cecal content_cecal bacteria transplantantion.8.fid
SUBJECT_SAMPLE_FACTORS           	GF.cecal.content_cecal.bacteria__9	GF.cecal.content_cecal.bacteria__9	Sample source:cecal content | Genotype:GF C57BL/6J mice | Treatment:cecal gavage from TCDF group	RAW_FILE_NAME=3.GF mice cecal content_cecal bacteria transplantantion.9.fid
SUBJECT_SAMPLE_FACTORS           	GF.cecal.content_cecal.bacteria__1	GF.cecal.content_cecal.bacteria__1	Sample source:cecal content | Genotype:GF C57BL/6J mice | Treatment:cecal gavage from vehicle group	RAW_FILE_NAME=3.GF mice cecal content_cecal bacteria transplantantion.1.fid
SUBJECT_SAMPLE_FACTORS           	GF.cecal.content_cecal.bacteria__2	GF.cecal.content_cecal.bacteria__2	Sample source:cecal content | Genotype:GF C57BL/6J mice | Treatment:cecal gavage from vehicle group	RAW_FILE_NAME=3.GF mice cecal content_cecal bacteria transplantantion.2.fid
SUBJECT_SAMPLE_FACTORS           	GF.cecal.content_cecal.bacteria__3	GF.cecal.content_cecal.bacteria__3	Sample source:cecal content | Genotype:GF C57BL/6J mice | Treatment:cecal gavage from vehicle group	RAW_FILE_NAME=3.GF mice cecal content_cecal bacteria transplantantion.3.fid
SUBJECT_SAMPLE_FACTORS           	GF.cecal.content_cecal.bacteria__4	GF.cecal.content_cecal.bacteria__4	Sample source:cecal content | Genotype:GF C57BL/6J mice | Treatment:cecal gavage from vehicle group	RAW_FILE_NAME=3.GF mice cecal content_cecal bacteria transplantantion.4.fid
SUBJECT_SAMPLE_FACTORS           	cecal.content.with.TCDF_long__10	cecal.content.with.TCDF_long__10	Sample source:cecal content | Genotype:WT C57BL/6J mice | Treatment:TCDF	RAW_FILE_NAME=7.mice cecal content with TCDF_long.10.fid
SUBJECT_SAMPLE_FACTORS           	cecal.content.with.TCDF_long__11	cecal.content.with.TCDF_long__11	Sample source:cecal content | Genotype:WT C57BL/6J mice | Treatment:TCDF	RAW_FILE_NAME=7.mice cecal content with TCDF_long.11.fid
SUBJECT_SAMPLE_FACTORS           	cecal.content.with.TCDF_long__12	cecal.content.with.TCDF_long__12	Sample source:cecal content | Genotype:WT C57BL/6J mice | Treatment:TCDF	RAW_FILE_NAME=7.mice cecal content with TCDF_long.12.fid
SUBJECT_SAMPLE_FACTORS           	cecal.content.with.TCDF_long__7	cecal.content.with.TCDF_long__7	Sample source:cecal content | Genotype:WT C57BL/6J mice | Treatment:TCDF	RAW_FILE_NAME=7.mice cecal content with TCDF_long.7.fid
SUBJECT_SAMPLE_FACTORS           	cecal.content.with.TCDF_long__8	cecal.content.with.TCDF_long__8	Sample source:cecal content | Genotype:WT C57BL/6J mice | Treatment:TCDF	RAW_FILE_NAME=7.mice cecal content with TCDF_long.8.fid
SUBJECT_SAMPLE_FACTORS           	cecal.content.with.TCDF_long__9	cecal.content.with.TCDF_long__9	Sample source:cecal content | Genotype:WT C57BL/6J mice | Treatment:TCDF	RAW_FILE_NAME=7.mice cecal content with TCDF_long.9.fid
SUBJECT_SAMPLE_FACTORS           	cecal.content.with.TCDF_short__10	cecal.content.with.TCDF_short__10	Sample source:cecal content | Genotype:WT C57BL/6J mice | Treatment:TCDF	RAW_FILE_NAME=8.mice cecal content with TCDF_short.10.fid
SUBJECT_SAMPLE_FACTORS           	cecal.content.with.TCDF_short__11	cecal.content.with.TCDF_short__11	Sample source:cecal content | Genotype:WT C57BL/6J mice | Treatment:TCDF	RAW_FILE_NAME=8.mice cecal content with TCDF_short.11.fid
SUBJECT_SAMPLE_FACTORS           	cecal.content.with.TCDF_short__12	cecal.content.with.TCDF_short__12	Sample source:cecal content | Genotype:WT C57BL/6J mice | Treatment:TCDF	RAW_FILE_NAME=8.mice cecal content with TCDF_short.12.fid
SUBJECT_SAMPLE_FACTORS           	cecal.content.with.TCDF_short__7	cecal.content.with.TCDF_short__7	Sample source:cecal content | Genotype:WT C57BL/6J mice | Treatment:TCDF	RAW_FILE_NAME=8.mice cecal content with TCDF_short.7.fid
SUBJECT_SAMPLE_FACTORS           	cecal.content.with.TCDF_short__8	cecal.content.with.TCDF_short__8	Sample source:cecal content | Genotype:WT C57BL/6J mice | Treatment:TCDF	RAW_FILE_NAME=8.mice cecal content with TCDF_short.8.fid
SUBJECT_SAMPLE_FACTORS           	cecal.content.with.TCDF_short__9	cecal.content.with.TCDF_short__9	Sample source:cecal content | Genotype:WT C57BL/6J mice | Treatment:TCDF	RAW_FILE_NAME=8.mice cecal content with TCDF_short.9.fid
SUBJECT_SAMPLE_FACTORS           	cecal.content.with.TCDF_long__1	cecal.content.with.TCDF_long__1	Sample source:cecal content | Genotype:WT C57BL/6J mice | Treatment:vehicle	RAW_FILE_NAME=7.mice cecal content with TCDF_long.1.fid
SUBJECT_SAMPLE_FACTORS           	cecal.content.with.TCDF_long__2	cecal.content.with.TCDF_long__2	Sample source:cecal content | Genotype:WT C57BL/6J mice | Treatment:vehicle	RAW_FILE_NAME=7.mice cecal content with TCDF_long.2.fid
SUBJECT_SAMPLE_FACTORS           	cecal.content.with.TCDF_long__3	cecal.content.with.TCDF_long__3	Sample source:cecal content | Genotype:WT C57BL/6J mice | Treatment:vehicle	RAW_FILE_NAME=7.mice cecal content with TCDF_long.3.fid
SUBJECT_SAMPLE_FACTORS           	cecal.content.with.TCDF_long__4	cecal.content.with.TCDF_long__4	Sample source:cecal content | Genotype:WT C57BL/6J mice | Treatment:vehicle	RAW_FILE_NAME=7.mice cecal content with TCDF_long.4.fid
SUBJECT_SAMPLE_FACTORS           	cecal.content.with.TCDF_long__5	cecal.content.with.TCDF_long__5	Sample source:cecal content | Genotype:WT C57BL/6J mice | Treatment:vehicle	RAW_FILE_NAME=7.mice cecal content with TCDF_long.5.fid
SUBJECT_SAMPLE_FACTORS           	cecal.content.with.TCDF_long__6	cecal.content.with.TCDF_long__6	Sample source:cecal content | Genotype:WT C57BL/6J mice | Treatment:vehicle	RAW_FILE_NAME=7.mice cecal content with TCDF_long.6.fid
SUBJECT_SAMPLE_FACTORS           	cecal.content.with.TCDF_short__1	cecal.content.with.TCDF_short__1	Sample source:cecal content | Genotype:WT C57BL/6J mice | Treatment:vehicle	RAW_FILE_NAME=8.mice cecal content with TCDF_short.1.fid
SUBJECT_SAMPLE_FACTORS           	cecal.content.with.TCDF_short__2	cecal.content.with.TCDF_short__2	Sample source:cecal content | Genotype:WT C57BL/6J mice | Treatment:vehicle	RAW_FILE_NAME=8.mice cecal content with TCDF_short.2.fid
SUBJECT_SAMPLE_FACTORS           	cecal.content.with.TCDF_short__3	cecal.content.with.TCDF_short__3	Sample source:cecal content | Genotype:WT C57BL/6J mice | Treatment:vehicle	RAW_FILE_NAME=8.mice cecal content with TCDF_short.3.fid
SUBJECT_SAMPLE_FACTORS           	cecal.content.with.TCDF_short__4	cecal.content.with.TCDF_short__4	Sample source:cecal content | Genotype:WT C57BL/6J mice | Treatment:vehicle	RAW_FILE_NAME=8.mice cecal content with TCDF_short.4.fid
SUBJECT_SAMPLE_FACTORS           	cecal.content.with.TCDF_short__5	cecal.content.with.TCDF_short__5	Sample source:cecal content | Genotype:WT C57BL/6J mice | Treatment:vehicle	RAW_FILE_NAME=8.mice cecal content with TCDF_short.5.fid
SUBJECT_SAMPLE_FACTORS           	cecal.content.with.TCDF_short__6	cecal.content.with.TCDF_short__6	Sample source:cecal content | Genotype:WT C57BL/6J mice | Treatment:vehicle	RAW_FILE_NAME=8.mice cecal content with TCDF_short.6.fid
SUBJECT_SAMPLE_FACTORS           	AHR.KO.liver.lipid.with.TCDF__4	AHR.KO.liver.lipid.with.TCDF__4	Sample source:liver | Genotype:Ahr-/- C57BL/6J mice | Treatment:TCDF	RAW_FILE_NAME=1.AHR KO mice liver lipid with TCDF.4.fid
SUBJECT_SAMPLE_FACTORS           	AHR.KO.liver.lipid.with.TCDF__5	AHR.KO.liver.lipid.with.TCDF__5	Sample source:liver | Genotype:Ahr-/- C57BL/6J mice | Treatment:TCDF	RAW_FILE_NAME=1.AHR KO mice liver lipid with TCDF.5.fid
SUBJECT_SAMPLE_FACTORS           	AHR.KO.liver.lipid.with.TCDF__6	AHR.KO.liver.lipid.with.TCDF__6	Sample source:liver | Genotype:Ahr-/- C57BL/6J mice | Treatment:TCDF	RAW_FILE_NAME=1.AHR KO mice liver lipid with TCDF.6.fid
SUBJECT_SAMPLE_FACTORS           	AHR.KO.liver.lipid.with.TCDF__8	AHR.KO.liver.lipid.with.TCDF__8	Sample source:liver | Genotype:Ahr-/- C57BL/6J mice | Treatment:TCDF	RAW_FILE_NAME=1.AHR KO mice liver lipid with TCDF.8.fid
SUBJECT_SAMPLE_FACTORS           	AHR.KO.liver.lipid.with.TCDF__1	AHR.KO.liver.lipid.with.TCDF__1	Sample source:liver | Genotype:Ahr-/- C57BL/6J mice | Treatment:vehicle	RAW_FILE_NAME=1.AHR KO mice liver lipid with TCDF.1.fid
SUBJECT_SAMPLE_FACTORS           	AHR.KO.liver.lipid.with.TCDF__2	AHR.KO.liver.lipid.with.TCDF__2	Sample source:liver | Genotype:Ahr-/- C57BL/6J mice | Treatment:vehicle	RAW_FILE_NAME=1.AHR KO mice liver lipid with TCDF.2.fid
SUBJECT_SAMPLE_FACTORS           	AHR.KO.liver.lipid.with.TCDF__3	AHR.KO.liver.lipid.with.TCDF__3	Sample source:liver | Genotype:Ahr-/- C57BL/6J mice | Treatment:vehicle	RAW_FILE_NAME=1.AHR KO mice liver lipid with TCDF.3.fid
SUBJECT_SAMPLE_FACTORS           	AHR.KO.liver.lipid.with.TCDF__7	AHR.KO.liver.lipid.with.TCDF__7	Sample source:liver | Genotype:Ahr-/- C57BL/6J mice | Treatment:vehicle	RAW_FILE_NAME=1.AHR KO mice liver lipid with TCDF.7.fid
SUBJECT_SAMPLE_FACTORS           	GF.liver.lipid._cecal.bacteria__10	GF.liver.lipid._cecal.bacteria__10	Sample source:liver | Genotype:GF C57BL/6J mice | Treatment:cecal gavage from TCDF group	RAW_FILE_NAME=4.GF mice liver lipid _cecal bacteria transplantantion.10.fid
SUBJECT_SAMPLE_FACTORS           	GF.liver.lipid._cecal.bacteria__6	GF.liver.lipid._cecal.bacteria__6	Sample source:liver | Genotype:GF C57BL/6J mice | Treatment:cecal gavage from TCDF group	RAW_FILE_NAME=4.GF mice liver lipid _cecal bacteria transplantantion.6.fid
SUBJECT_SAMPLE_FACTORS           	GF.liver.lipid._cecal.bacteria__7	GF.liver.lipid._cecal.bacteria__7	Sample source:liver | Genotype:GF C57BL/6J mice | Treatment:cecal gavage from TCDF group	RAW_FILE_NAME=4.GF mice liver lipid _cecal bacteria transplantantion.7.fid
SUBJECT_SAMPLE_FACTORS           	GF.liver.lipid._cecal.bacteria__8	GF.liver.lipid._cecal.bacteria__8	Sample source:liver | Genotype:GF C57BL/6J mice | Treatment:cecal gavage from TCDF group	RAW_FILE_NAME=4.GF mice liver lipid _cecal bacteria transplantantion.8.fid
SUBJECT_SAMPLE_FACTORS           	GF.liver.lipid._cecal.bacteria__9	GF.liver.lipid._cecal.bacteria__9	Sample source:liver | Genotype:GF C57BL/6J mice | Treatment:cecal gavage from TCDF group	RAW_FILE_NAME=4.GF mice liver lipid _cecal bacteria transplantantion.9.fid
SUBJECT_SAMPLE_FACTORS           	GF.liver.lipid._cecal.bacteria__1	GF.liver.lipid._cecal.bacteria__1	Sample source:liver | Genotype:GF C57BL/6J mice | Treatment:cecal gavage from vehicle group	RAW_FILE_NAME=4.GF mice liver lipid _cecal bacteria transplantantion.1.fid
SUBJECT_SAMPLE_FACTORS           	GF.liver.lipid._cecal.bacteria__2	GF.liver.lipid._cecal.bacteria__2	Sample source:liver | Genotype:GF C57BL/6J mice | Treatment:cecal gavage from vehicle group	RAW_FILE_NAME=4.GF mice liver lipid _cecal bacteria transplantantion.2.fid
SUBJECT_SAMPLE_FACTORS           	GF.liver.lipid._cecal.bacteria__3	GF.liver.lipid._cecal.bacteria__3	Sample source:liver | Genotype:GF C57BL/6J mice | Treatment:cecal gavage from vehicle group	RAW_FILE_NAME=4.GF mice liver lipid _cecal bacteria transplantantion.3.fid
SUBJECT_SAMPLE_FACTORS           	GF.liver.lipid._cecal.bacteria__4	GF.liver.lipid._cecal.bacteria__4	Sample source:liver | Genotype:GF C57BL/6J mice | Treatment:cecal gavage from vehicle group	RAW_FILE_NAME=4.GF mice liver lipid _cecal bacteria transplantantion.4.fid
SUBJECT_SAMPLE_FACTORS           	GF.liver.lipid._cecal.bacteria__5	GF.liver.lipid._cecal.bacteria__5	Sample source:liver | Genotype:GF C57BL/6J mice | Treatment:cecal gavage from vehicle group	RAW_FILE_NAME=4.GF mice liver lipid _cecal bacteria transplantantion.5.fid
SUBJECT_SAMPLE_FACTORS           	GF.liver.lipid.with.TCDF__5	GF.liver.lipid.with.TCDF__5	Sample source:liver | Genotype:GF C57BL/6J mice | Treatment:TCDF	RAW_FILE_NAME=5.GF mice liver lipid with TCDF.5.fid
SUBJECT_SAMPLE_FACTORS           	GF.liver.lipid.with.TCDF__6	GF.liver.lipid.with.TCDF__6	Sample source:liver | Genotype:GF C57BL/6J mice | Treatment:TCDF	RAW_FILE_NAME=5.GF mice liver lipid with TCDF.6.fid
SUBJECT_SAMPLE_FACTORS           	GF.liver.lipid.with.TCDF__7	GF.liver.lipid.with.TCDF__7	Sample source:liver | Genotype:GF C57BL/6J mice | Treatment:TCDF	RAW_FILE_NAME=5.GF mice liver lipid with TCDF.7.fid
SUBJECT_SAMPLE_FACTORS           	GF.liver.lipid.with.TCDF__8	GF.liver.lipid.with.TCDF__8	Sample source:liver | Genotype:GF C57BL/6J mice | Treatment:TCDF	RAW_FILE_NAME=5.GF mice liver lipid with TCDF.8.fid
SUBJECT_SAMPLE_FACTORS           	GF.liver.lipid.with.TCDF__1	GF.liver.lipid.with.TCDF__1	Sample source:liver | Genotype:GF C57BL/6J mice | Treatment:vehicle	RAW_FILE_NAME=5.GF mice liver lipid with TCDF.1.fid
SUBJECT_SAMPLE_FACTORS           	GF.liver.lipid.with.TCDF__2	GF.liver.lipid.with.TCDF__2	Sample source:liver | Genotype:GF C57BL/6J mice | Treatment:vehicle	RAW_FILE_NAME=5.GF mice liver lipid with TCDF.2.fid
SUBJECT_SAMPLE_FACTORS           	GF.liver.lipid.with.TCDF__3	GF.liver.lipid.with.TCDF__3	Sample source:liver | Genotype:GF C57BL/6J mice | Treatment:vehicle	RAW_FILE_NAME=5.GF mice liver lipid with TCDF.3.fid
SUBJECT_SAMPLE_FACTORS           	GF.liver.lipid.with.TCDF__4	GF.liver.lipid.with.TCDF__4	Sample source:liver | Genotype:GF C57BL/6J mice | Treatment:vehicle	RAW_FILE_NAME=5.GF mice liver lipid with TCDF.4.fid
SUBJECT_SAMPLE_FACTORS           	liver.lipid.with.TCDF_long__207	liver.lipid.with.TCDF_long__207	Sample source:liver | Genotype:WT C57BL/6J mice | Treatment:TCDF	RAW_FILE_NAME=9.mice liver lipid with TCDF_long.207.fid
SUBJECT_SAMPLE_FACTORS           	liver.lipid.with.TCDF_long__208	liver.lipid.with.TCDF_long__208	Sample source:liver | Genotype:WT C57BL/6J mice | Treatment:TCDF	RAW_FILE_NAME=9.mice liver lipid with TCDF_long.208.fid
SUBJECT_SAMPLE_FACTORS           	liver.lipid.with.TCDF_long__209	liver.lipid.with.TCDF_long__209	Sample source:liver | Genotype:WT C57BL/6J mice | Treatment:TCDF	RAW_FILE_NAME=9.mice liver lipid with TCDF_long.209.fid
SUBJECT_SAMPLE_FACTORS           	liver.lipid.with.TCDF_long__210	liver.lipid.with.TCDF_long__210	Sample source:liver | Genotype:WT C57BL/6J mice | Treatment:TCDF	RAW_FILE_NAME=9.mice liver lipid with TCDF_long.210.fid
SUBJECT_SAMPLE_FACTORS           	liver.lipid.with.TCDF_long__211	liver.lipid.with.TCDF_long__211	Sample source:liver | Genotype:WT C57BL/6J mice | Treatment:TCDF	RAW_FILE_NAME=9.mice liver lipid with TCDF_long.211.fid
SUBJECT_SAMPLE_FACTORS           	liver.lipid.with.TCDF_long__212	liver.lipid.with.TCDF_long__212	Sample source:liver | Genotype:WT C57BL/6J mice | Treatment:TCDF	RAW_FILE_NAME=9.mice liver lipid with TCDF_long.212.fid
SUBJECT_SAMPLE_FACTORS           	liver.lipid.with.TCDF_short__107	liver.lipid.with.TCDF_short__107	Sample source:liver | Genotype:WT C57BL/6J mice | Treatment:TCDF	RAW_FILE_NAME=10.mice liver lipid with TCDF_short.107.fid
SUBJECT_SAMPLE_FACTORS           	liver.lipid.with.TCDF_short__108	liver.lipid.with.TCDF_short__108	Sample source:liver | Genotype:WT C57BL/6J mice | Treatment:TCDF	RAW_FILE_NAME=10.mice liver lipid with TCDF_short.108.fid
SUBJECT_SAMPLE_FACTORS           	liver.lipid.with.TCDF_short__109	liver.lipid.with.TCDF_short__109	Sample source:liver | Genotype:WT C57BL/6J mice | Treatment:TCDF	RAW_FILE_NAME=10.mice liver lipid with TCDF_short.109.fid
SUBJECT_SAMPLE_FACTORS           	liver.lipid.with.TCDF_short__110	liver.lipid.with.TCDF_short__110	Sample source:liver | Genotype:WT C57BL/6J mice | Treatment:TCDF	RAW_FILE_NAME=10.mice liver lipid with TCDF_short.110.fid
SUBJECT_SAMPLE_FACTORS           	liver.lipid.with.TCDF_short__111	liver.lipid.with.TCDF_short__111	Sample source:liver | Genotype:WT C57BL/6J mice | Treatment:TCDF	RAW_FILE_NAME=10.mice liver lipid with TCDF_short.111.fid
SUBJECT_SAMPLE_FACTORS           	liver.lipid.with.TCDF_short__112	liver.lipid.with.TCDF_short__112	Sample source:liver | Genotype:WT C57BL/6J mice | Treatment:TCDF	RAW_FILE_NAME=10.mice liver lipid with TCDF_short.112.fid
SUBJECT_SAMPLE_FACTORS           	liver.with.TCDF_.long__10	liver.with.TCDF_.long__10	Sample source:liver | Genotype:WT C57BL/6J mice | Treatment:TCDF	RAW_FILE_NAME=11.mice liver with TCDF_ long.10.fid
SUBJECT_SAMPLE_FACTORS           	liver.with.TCDF_.long__11	liver.with.TCDF_.long__11	Sample source:liver | Genotype:WT C57BL/6J mice | Treatment:TCDF	RAW_FILE_NAME=11.mice liver with TCDF_ long.11.fid
SUBJECT_SAMPLE_FACTORS           	liver.with.TCDF_.long__12	liver.with.TCDF_.long__12	Sample source:liver | Genotype:WT C57BL/6J mice | Treatment:TCDF	RAW_FILE_NAME=11.mice liver with TCDF_ long.12.fid
SUBJECT_SAMPLE_FACTORS           	liver.with.TCDF_.long__7	liver.with.TCDF_.long__7	Sample source:liver | Genotype:WT C57BL/6J mice | Treatment:TCDF	RAW_FILE_NAME=11.mice liver with TCDF_ long.7.fid
SUBJECT_SAMPLE_FACTORS           	liver.with.TCDF_.long__8	liver.with.TCDF_.long__8	Sample source:liver | Genotype:WT C57BL/6J mice | Treatment:TCDF	RAW_FILE_NAME=11.mice liver with TCDF_ long.8.fid
SUBJECT_SAMPLE_FACTORS           	liver.with.TCDF_.long__9	liver.with.TCDF_.long__9	Sample source:liver | Genotype:WT C57BL/6J mice | Treatment:TCDF	RAW_FILE_NAME=11.mice liver with TCDF_ long.9.fid
SUBJECT_SAMPLE_FACTORS           	liver.with.TCDF_short__10	liver.with.TCDF_short__10	Sample source:liver | Genotype:WT C57BL/6J mice | Treatment:TCDF	RAW_FILE_NAME=12.mice liver with TCDF_short.10.fid
SUBJECT_SAMPLE_FACTORS           	liver.with.TCDF_short__11	liver.with.TCDF_short__11	Sample source:liver | Genotype:WT C57BL/6J mice | Treatment:TCDF	RAW_FILE_NAME=12.mice liver with TCDF_short.11.fid
SUBJECT_SAMPLE_FACTORS           	liver.with.TCDF_short__12	liver.with.TCDF_short__12	Sample source:liver | Genotype:WT C57BL/6J mice | Treatment:TCDF	RAW_FILE_NAME=12.mice liver with TCDF_short.12.fid
SUBJECT_SAMPLE_FACTORS           	liver.with.TCDF_short__7	liver.with.TCDF_short__7	Sample source:liver | Genotype:WT C57BL/6J mice | Treatment:TCDF	RAW_FILE_NAME=12.mice liver with TCDF_short.7.fid
SUBJECT_SAMPLE_FACTORS           	liver.with.TCDF_short__8	liver.with.TCDF_short__8	Sample source:liver | Genotype:WT C57BL/6J mice | Treatment:TCDF	RAW_FILE_NAME=12.mice liver with TCDF_short.8.fid
SUBJECT_SAMPLE_FACTORS           	liver.with.TCDF_short__9	liver.with.TCDF_short__9	Sample source:liver | Genotype:WT C57BL/6J mice | Treatment:TCDF	RAW_FILE_NAME=12.mice liver with TCDF_short.9.fid
SUBJECT_SAMPLE_FACTORS           	liver.lipid.with.TCDF_long__201	liver.lipid.with.TCDF_long__201	Sample source:liver | Genotype:WT C57BL/6J mice | Treatment:vehicle	RAW_FILE_NAME=9.mice liver lipid with TCDF_long.201.fid
SUBJECT_SAMPLE_FACTORS           	liver.lipid.with.TCDF_long__202	liver.lipid.with.TCDF_long__202	Sample source:liver | Genotype:WT C57BL/6J mice | Treatment:vehicle	RAW_FILE_NAME=9.mice liver lipid with TCDF_long.202.fid
SUBJECT_SAMPLE_FACTORS           	liver.lipid.with.TCDF_long__203	liver.lipid.with.TCDF_long__203	Sample source:liver | Genotype:WT C57BL/6J mice | Treatment:vehicle	RAW_FILE_NAME=9.mice liver lipid with TCDF_long.203.fid
SUBJECT_SAMPLE_FACTORS           	liver.lipid.with.TCDF_long__204	liver.lipid.with.TCDF_long__204	Sample source:liver | Genotype:WT C57BL/6J mice | Treatment:vehicle	RAW_FILE_NAME=9.mice liver lipid with TCDF_long.204.fid
SUBJECT_SAMPLE_FACTORS           	liver.lipid.with.TCDF_long__205	liver.lipid.with.TCDF_long__205	Sample source:liver | Genotype:WT C57BL/6J mice | Treatment:vehicle	RAW_FILE_NAME=9.mice liver lipid with TCDF_long.205.fid
SUBJECT_SAMPLE_FACTORS           	liver.lipid.with.TCDF_long__206	liver.lipid.with.TCDF_long__206	Sample source:liver | Genotype:WT C57BL/6J mice | Treatment:vehicle	RAW_FILE_NAME=9.mice liver lipid with TCDF_long.206.fid
SUBJECT_SAMPLE_FACTORS           	liver.lipid.with.TCDF_short__101	liver.lipid.with.TCDF_short__101	Sample source:liver | Genotype:WT C57BL/6J mice | Treatment:vehicle	RAW_FILE_NAME=10.mice liver lipid with TCDF_short.101.fid
SUBJECT_SAMPLE_FACTORS           	liver.lipid.with.TCDF_short__102	liver.lipid.with.TCDF_short__102	Sample source:liver | Genotype:WT C57BL/6J mice | Treatment:vehicle	RAW_FILE_NAME=10.mice liver lipid with TCDF_short.102.fid
SUBJECT_SAMPLE_FACTORS           	liver.lipid.with.TCDF_short__103	liver.lipid.with.TCDF_short__103	Sample source:liver | Genotype:WT C57BL/6J mice | Treatment:vehicle	RAW_FILE_NAME=10.mice liver lipid with TCDF_short.103.fid
SUBJECT_SAMPLE_FACTORS           	liver.lipid.with.TCDF_short__104	liver.lipid.with.TCDF_short__104	Sample source:liver | Genotype:WT C57BL/6J mice | Treatment:vehicle	RAW_FILE_NAME=10.mice liver lipid with TCDF_short.104.fid
SUBJECT_SAMPLE_FACTORS           	liver.lipid.with.TCDF_short__105	liver.lipid.with.TCDF_short__105	Sample source:liver | Genotype:WT C57BL/6J mice | Treatment:vehicle	RAW_FILE_NAME=10.mice liver lipid with TCDF_short.105.fid
SUBJECT_SAMPLE_FACTORS           	liver.lipid.with.TCDF_short__106	liver.lipid.with.TCDF_short__106	Sample source:liver | Genotype:WT C57BL/6J mice | Treatment:vehicle	RAW_FILE_NAME=10.mice liver lipid with TCDF_short.106.fid
SUBJECT_SAMPLE_FACTORS           	liver.with.TCDF_.long__1	liver.with.TCDF_.long__1	Sample source:liver | Genotype:WT C57BL/6J mice | Treatment:vehicle	RAW_FILE_NAME=11.mice liver with TCDF_ long.1.fid
SUBJECT_SAMPLE_FACTORS           	liver.with.TCDF_.long__2	liver.with.TCDF_.long__2	Sample source:liver | Genotype:WT C57BL/6J mice | Treatment:vehicle	RAW_FILE_NAME=11.mice liver with TCDF_ long.2.fid
SUBJECT_SAMPLE_FACTORS           	liver.with.TCDF_.long__3	liver.with.TCDF_.long__3	Sample source:liver | Genotype:WT C57BL/6J mice | Treatment:vehicle	RAW_FILE_NAME=11.mice liver with TCDF_ long.3.fid
SUBJECT_SAMPLE_FACTORS           	liver.with.TCDF_.long__4	liver.with.TCDF_.long__4	Sample source:liver | Genotype:WT C57BL/6J mice | Treatment:vehicle	RAW_FILE_NAME=11.mice liver with TCDF_ long.4.fid
SUBJECT_SAMPLE_FACTORS           	liver.with.TCDF_.long__5	liver.with.TCDF_.long__5	Sample source:liver | Genotype:WT C57BL/6J mice | Treatment:vehicle	RAW_FILE_NAME=11.mice liver with TCDF_ long.5.fid
SUBJECT_SAMPLE_FACTORS           	liver.with.TCDF_.long__6	liver.with.TCDF_.long__6	Sample source:liver | Genotype:WT C57BL/6J mice | Treatment:vehicle	RAW_FILE_NAME=11.mice liver with TCDF_ long.6.fid
SUBJECT_SAMPLE_FACTORS           	liver.with.TCDF_short__1	liver.with.TCDF_short__1	Sample source:liver | Genotype:WT C57BL/6J mice | Treatment:vehicle	RAW_FILE_NAME=12.mice liver with TCDF_short.1.fid
SUBJECT_SAMPLE_FACTORS           	liver.with.TCDF_short__2	liver.with.TCDF_short__2	Sample source:liver | Genotype:WT C57BL/6J mice | Treatment:vehicle	RAW_FILE_NAME=12.mice liver with TCDF_short.2.fid
SUBJECT_SAMPLE_FACTORS           	liver.with.TCDF_short__3	liver.with.TCDF_short__3	Sample source:liver | Genotype:WT C57BL/6J mice | Treatment:vehicle	RAW_FILE_NAME=12.mice liver with TCDF_short.3.fid
SUBJECT_SAMPLE_FACTORS           	liver.with.TCDF_short__4	liver.with.TCDF_short__4	Sample source:liver | Genotype:WT C57BL/6J mice | Treatment:vehicle	RAW_FILE_NAME=12.mice liver with TCDF_short.4.fid
SUBJECT_SAMPLE_FACTORS           	liver.with.TCDF_short__5	liver.with.TCDF_short__5	Sample source:liver | Genotype:WT C57BL/6J mice | Treatment:vehicle	RAW_FILE_NAME=12.mice liver with TCDF_short.5.fid
SUBJECT_SAMPLE_FACTORS           	liver.with.TCDF_short__6	liver.with.TCDF_short__6	Sample source:liver | Genotype:WT C57BL/6J mice | Treatment:vehicle	RAW_FILE_NAME=12.mice liver with TCDF_short.6.fid
SUBJECT_SAMPLE_FACTORS           	urine.with.TCDF_long__10	urine.with.TCDF_long__10	Sample source:urine | Genotype:WT C57BL/6J mice | Treatment:TCDF	RAW_FILE_NAME=13.mice urine with TCDF_long.10.fid
SUBJECT_SAMPLE_FACTORS           	urine.with.TCDF_long__11	urine.with.TCDF_long__11	Sample source:urine | Genotype:WT C57BL/6J mice | Treatment:TCDF	RAW_FILE_NAME=13.mice urine with TCDF_long.11.fid
SUBJECT_SAMPLE_FACTORS           	urine.with.TCDF_long__12	urine.with.TCDF_long__12	Sample source:urine | Genotype:WT C57BL/6J mice | Treatment:TCDF	RAW_FILE_NAME=13.mice urine with TCDF_long.12.fid
SUBJECT_SAMPLE_FACTORS           	urine.with.TCDF_long__7	urine.with.TCDF_long__7	Sample source:urine | Genotype:WT C57BL/6J mice | Treatment:TCDF	RAW_FILE_NAME=13.mice urine with TCDF_long.7.fid
SUBJECT_SAMPLE_FACTORS           	urine.with.TCDF_long__8	urine.with.TCDF_long__8	Sample source:urine | Genotype:WT C57BL/6J mice | Treatment:TCDF	RAW_FILE_NAME=13.mice urine with TCDF_long.8.fid
SUBJECT_SAMPLE_FACTORS           	urine.with.TCDF_long__9	urine.with.TCDF_long__9	Sample source:urine | Genotype:WT C57BL/6J mice | Treatment:TCDF	RAW_FILE_NAME=13.mice urine with TCDF_long.9.fid
SUBJECT_SAMPLE_FACTORS           	urine.with.TCDF_short__10	urine.with.TCDF_short__10	Sample source:urine | Genotype:WT C57BL/6J mice | Treatment:TCDF	RAW_FILE_NAME=14.mice urine with TCDF_short.10.fid
SUBJECT_SAMPLE_FACTORS           	urine.with.TCDF_short__11	urine.with.TCDF_short__11	Sample source:urine | Genotype:WT C57BL/6J mice | Treatment:TCDF	RAW_FILE_NAME=14.mice urine with TCDF_short.11.fid
SUBJECT_SAMPLE_FACTORS           	urine.with.TCDF_short__12	urine.with.TCDF_short__12	Sample source:urine | Genotype:WT C57BL/6J mice | Treatment:TCDF	RAW_FILE_NAME=14.mice urine with TCDF_short.12.fid
SUBJECT_SAMPLE_FACTORS           	urine.with.TCDF_short__7	urine.with.TCDF_short__7	Sample source:urine | Genotype:WT C57BL/6J mice | Treatment:TCDF	RAW_FILE_NAME=14.mice urine with TCDF_short.7.fid
SUBJECT_SAMPLE_FACTORS           	urine.with.TCDF_short__8	urine.with.TCDF_short__8	Sample source:urine | Genotype:WT C57BL/6J mice | Treatment:TCDF	RAW_FILE_NAME=14.mice urine with TCDF_short.8.fid
SUBJECT_SAMPLE_FACTORS           	urine.with.TCDF_short__9	urine.with.TCDF_short__9	Sample source:urine | Genotype:WT C57BL/6J mice | Treatment:TCDF	RAW_FILE_NAME=14.mice urine with TCDF_short.9.fid
SUBJECT_SAMPLE_FACTORS           	urine.with.TCDF_long__1	urine.with.TCDF_long__1	Sample source:urine | Genotype:WT C57BL/6J mice | Treatment:vehicle	RAW_FILE_NAME=13.mice urine with TCDF_long.1.fid
SUBJECT_SAMPLE_FACTORS           	urine.with.TCDF_long__2	urine.with.TCDF_long__2	Sample source:urine | Genotype:WT C57BL/6J mice | Treatment:vehicle	RAW_FILE_NAME=13.mice urine with TCDF_long.2.fid
SUBJECT_SAMPLE_FACTORS           	urine.with.TCDF_long__3	urine.with.TCDF_long__3	Sample source:urine | Genotype:WT C57BL/6J mice | Treatment:vehicle	RAW_FILE_NAME=13.mice urine with TCDF_long.3.fid
SUBJECT_SAMPLE_FACTORS           	urine.with.TCDF_long__4	urine.with.TCDF_long__4	Sample source:urine | Genotype:WT C57BL/6J mice | Treatment:vehicle	RAW_FILE_NAME=13.mice urine with TCDF_long.4.fid
SUBJECT_SAMPLE_FACTORS           	urine.with.TCDF_long__5	urine.with.TCDF_long__5	Sample source:urine | Genotype:WT C57BL/6J mice | Treatment:vehicle	RAW_FILE_NAME=13.mice urine with TCDF_long.5.fid
SUBJECT_SAMPLE_FACTORS           	urine.with.TCDF_long__6	urine.with.TCDF_long__6	Sample source:urine | Genotype:WT C57BL/6J mice | Treatment:vehicle	RAW_FILE_NAME=13.mice urine with TCDF_long.6.fid
SUBJECT_SAMPLE_FACTORS           	urine.with.TCDF_short__1	urine.with.TCDF_short__1	Sample source:urine | Genotype:WT C57BL/6J mice | Treatment:vehicle	RAW_FILE_NAME=14.mice urine with TCDF_short.1.fid
SUBJECT_SAMPLE_FACTORS           	urine.with.TCDF_short__2	urine.with.TCDF_short__2	Sample source:urine | Genotype:WT C57BL/6J mice | Treatment:vehicle	RAW_FILE_NAME=14.mice urine with TCDF_short.2.fid
SUBJECT_SAMPLE_FACTORS           	urine.with.TCDF_short__3	urine.with.TCDF_short__3	Sample source:urine | Genotype:WT C57BL/6J mice | Treatment:vehicle	RAW_FILE_NAME=14.mice urine with TCDF_short.3.fid
SUBJECT_SAMPLE_FACTORS           	urine.with.TCDF_short__4	urine.with.TCDF_short__4	Sample source:urine | Genotype:WT C57BL/6J mice | Treatment:vehicle	RAW_FILE_NAME=14.mice urine with TCDF_short.4.fid
SUBJECT_SAMPLE_FACTORS           	urine.with.TCDF_short__5	urine.with.TCDF_short__5	Sample source:urine | Genotype:WT C57BL/6J mice | Treatment:vehicle	RAW_FILE_NAME=14.mice urine with TCDF_short.5.fid
SUBJECT_SAMPLE_FACTORS           	urine.with.TCDF_short__6	urine.with.TCDF_short__6	Sample source:urine | Genotype:WT C57BL/6J mice | Treatment:vehicle	RAW_FILE_NAME=14.mice urine with TCDF_short.6.fid
#COLLECTION
CO:COLLECTION_SUMMARY            	Liver, cecal content, and intestinal tissue samples were collected immediately
CO:COLLECTION_SUMMARY            	after sacrifice by carbon dioxide asphyxiation and kept at -80°C. Urine and
CO:COLLECTION_SUMMARY            	feces were collected using separate cages before sacrifice and kept at -80°C.
CO:SAMPLE_TYPE                   	liver, cecal content, urine
#TREATMENT
TR:TREATMENT_SUMMARY             	1. After feed training, mice were fed pills containing TCDF (dissolved in
TR:TREATMENT_SUMMARY             	acetone) or acetone alone as vehicle, and one pill uniformly contained 0.46 µg
TR:TREATMENT_SUMMARY             	TCDF (24 µg/kg as final dose). Mice were housed singly in an empty cage and
TR:TREATMENT_SUMMARY             	monitored to ensure the pill was consumed in the morning for 5 days. 2. TCDF (24
TR:TREATMENT_SUMMARY             	µg/kg) or corn oil as vehicle were administered to age-matched male GF and
TR:TREATMENT_SUMMARY             	Ahr-/- mice by oral gavage once daily for five days (n = 4 per group). 3.
TR:TREATMENT_SUMMARY             	Four-week-old male GF C57BL/6J mice were orally gavaged with 100 µL of cecal
TR:TREATMENT_SUMMARY             	suspension (100 mg in 1 mL sterile BHI CHV media) from vehicle or TCDF treated
TR:TREATMENT_SUMMARY             	mice in long-duration model. 4. A. muciniphila was administered to GF mice by
TR:TREATMENT_SUMMARY             	oral gavage at one dose 107 CFU/0.1 mL suspended in sterile BHI CHV media
TR:TREATMENT_SUMMARY             	containing an end concentration of glycerol (15% vol/vol).
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	For preparation of urine samples, 550 μL urine was blended with 55 μL of
SP:SAMPLEPREP_SUMMARY            	phosphate buffer (K2HPO4/NaH2PO4, 1.5 M, pH 7.4, 100% D2O) containing 0.01% TSP
SP:SAMPLEPREP_SUMMARY            	for chemical shift reference (δ0.00). After centrifugation (11 180 ×g, 4
SP:SAMPLEPREP_SUMMARY            	°C) for 10 min, 550 μL supernatant was transferred into 5 mm NMR tubes for NMR
SP:SAMPLEPREP_SUMMARY            	analysis. Each liver tissue sample (55 ± 5 mg) was extracted three times with
SP:SAMPLEPREP_SUMMARY            	600 μL of methanol/H2O (2:1) using a Qiagen tissue-lyzer. Three resultant
SP:SAMPLEPREP_SUMMARY            	supernatants were combined and subjected to centrifugation (16 099 ×g, 4 °C,
SP:SAMPLEPREP_SUMMARY            	10 min). After removing methanol in vacuo, the resultant supernatant was
SP:SAMPLEPREP_SUMMARY            	lyophilized, weighed, and reconstituted into 600 μL phosphate buffer (0.1 M,
SP:SAMPLEPREP_SUMMARY            	K2HPO4/NaH2PO4, pH 7.4) containing 0.005% TSP, 50% D2O and 0.01% NaN3. After
SP:SAMPLEPREP_SUMMARY            	final centrifugation (16 099 ×g, 4 °C, 10 min), each supernatant (550 μL)
SP:SAMPLEPREP_SUMMARY            	was transferred into a 5 mm NMR tube for NMR analysis. Each fecal sample (55 ±
SP:SAMPLEPREP_SUMMARY            	5 mg) were extracted twice with 500 μL phosphate buffer (0.1 M, K2HPO4/NaH2PO4,
SP:SAMPLEPREP_SUMMARY            	pH 7.4) containing 0.005% TSP, 30% D2O and 0.01% NaN3. After centrifugation
SP:SAMPLEPREP_SUMMARY            	(16 099 ×g, 4 °C, 10 min), 600 μL combined supernatants were transferred
SP:SAMPLEPREP_SUMMARY            	into 5 mm NMR tubes for NMR analysis. For liver lipid quantification, 50 mg of
SP:SAMPLEPREP_SUMMARY            	liver tissue was homogenized in 1 mL pre-cooled chloroform/methanol mix [1:1
SP:SAMPLEPREP_SUMMARY            	(v/v)], followed by adding 296 µL water. After centrifugation (10,000 g, 4°C)
SP:SAMPLEPREP_SUMMARY            	for 10 min, the lower phase was dried in a vacuum and reconstituted in 600 µL
SP:SAMPLEPREP_SUMMARY            	of deuterated chloroform containing 0.03% (v/v) tetramethylsilane (TMS).
#CHROMATOGRAPHY
#ANALYSIS
AN:ANALYSIS_TYPE                 	NMR
AN:ANALYSIS_PROTOCOL_FILE        	PSU_Protocol_for_NMR_metabolomics.pdf
#NMR
NM:INSTRUMENT_NAME               	Bruker Avance NEO 600 MHz
NM:INSTRUMENT_TYPE               	FT-NMR
NM:NMR_EXPERIMENT_TYPE           	1D-1H
NM:SPECTROMETER_FREQUENCY        	600 MHz
#NMR_METABOLITE_DATA
NMR_METABOLITE_DATA:UNITS        	µmol/g
NMR_METABOLITE_DATA_START
Samples	GF.cecal.content_Akkermansia__10	GF.cecal.content_Akkermansia__8	GF.cecal.content_Akkermansia__9	GF.cecal.content_Akkermansia__1	GF.cecal.content_Akkermansia__2	GF.cecal.content_Akkermansia__3	GF.cecal.content_Akkermansia__4	GF.cecal.content_Akkermansia__5	GF.cecal.content_Akkermansia__11	GF.cecal.content_Akkermansia__12	GF.cecal.content_Akkermansia__6	GF.cecal.content_Akkermansia__7	GF.cecal.content_cecal.bacteria__6	GF.cecal.content_cecal.bacteria__7	GF.cecal.content_cecal.bacteria__8	GF.cecal.content_cecal.bacteria__9	GF.cecal.content_cecal.bacteria__1	GF.cecal.content_cecal.bacteria__2	GF.cecal.content_cecal.bacteria__3	GF.cecal.content_cecal.bacteria__4	cecal.content.with.TCDF_long__10	cecal.content.with.TCDF_long__11	cecal.content.with.TCDF_long__12	cecal.content.with.TCDF_long__7	cecal.content.with.TCDF_long__8	cecal.content.with.TCDF_long__9	cecal.content.with.TCDF_short__10	cecal.content.with.TCDF_short__11	cecal.content.with.TCDF_short__12	cecal.content.with.TCDF_short__7	cecal.content.with.TCDF_short__8	cecal.content.with.TCDF_short__9	cecal.content.with.TCDF_long__1	cecal.content.with.TCDF_long__2	cecal.content.with.TCDF_long__3	cecal.content.with.TCDF_long__4	cecal.content.with.TCDF_long__5	cecal.content.with.TCDF_long__6	cecal.content.with.TCDF_short__1	cecal.content.with.TCDF_short__2	cecal.content.with.TCDF_short__3	cecal.content.with.TCDF_short__4	cecal.content.with.TCDF_short__5	cecal.content.with.TCDF_short__6	AHR.KO.liver.lipid.with.TCDF__4	AHR.KO.liver.lipid.with.TCDF__5	AHR.KO.liver.lipid.with.TCDF__6	AHR.KO.liver.lipid.with.TCDF__8	AHR.KO.liver.lipid.with.TCDF__1	AHR.KO.liver.lipid.with.TCDF__2	AHR.KO.liver.lipid.with.TCDF__3	AHR.KO.liver.lipid.with.TCDF__7	GF.liver.lipid._cecal.bacteria__10	GF.liver.lipid._cecal.bacteria__6	GF.liver.lipid._cecal.bacteria__7	GF.liver.lipid._cecal.bacteria__8	GF.liver.lipid._cecal.bacteria__9	GF.liver.lipid._cecal.bacteria__1	GF.liver.lipid._cecal.bacteria__2	GF.liver.lipid._cecal.bacteria__3	GF.liver.lipid._cecal.bacteria__4	GF.liver.lipid._cecal.bacteria__5	GF.liver.lipid.with.TCDF__5	GF.liver.lipid.with.TCDF__6	GF.liver.lipid.with.TCDF__7	GF.liver.lipid.with.TCDF__8	GF.liver.lipid.with.TCDF__1	GF.liver.lipid.with.TCDF__2	GF.liver.lipid.with.TCDF__3	GF.liver.lipid.with.TCDF__4	liver.lipid.with.TCDF_long__207	liver.lipid.with.TCDF_long__208	liver.lipid.with.TCDF_long__209	liver.lipid.with.TCDF_long__210	liver.lipid.with.TCDF_long__211	liver.lipid.with.TCDF_long__212	liver.lipid.with.TCDF_short__107	liver.lipid.with.TCDF_short__108	liver.lipid.with.TCDF_short__109	liver.lipid.with.TCDF_short__110	liver.lipid.with.TCDF_short__111	liver.lipid.with.TCDF_short__112	liver.with.TCDF_.long__10	liver.with.TCDF_.long__11	liver.with.TCDF_.long__12	liver.with.TCDF_.long__7	liver.with.TCDF_.long__8	liver.with.TCDF_.long__9	liver.with.TCDF_short__10	liver.with.TCDF_short__11	liver.with.TCDF_short__12	liver.with.TCDF_short__7	liver.with.TCDF_short__8	liver.with.TCDF_short__9	liver.lipid.with.TCDF_long__201	liver.lipid.with.TCDF_long__202	liver.lipid.with.TCDF_long__203	liver.lipid.with.TCDF_long__204	liver.lipid.with.TCDF_long__205	liver.lipid.with.TCDF_long__206	liver.lipid.with.TCDF_short__101	liver.lipid.with.TCDF_short__102	liver.lipid.with.TCDF_short__103	liver.lipid.with.TCDF_short__104	liver.lipid.with.TCDF_short__105	liver.lipid.with.TCDF_short__106	liver.with.TCDF_.long__1	liver.with.TCDF_.long__2	liver.with.TCDF_.long__3	liver.with.TCDF_.long__4	liver.with.TCDF_.long__5	liver.with.TCDF_.long__6	liver.with.TCDF_short__1	liver.with.TCDF_short__2	liver.with.TCDF_short__3	liver.with.TCDF_short__4	liver.with.TCDF_short__5	liver.with.TCDF_short__6	urine.with.TCDF_long__10	urine.with.TCDF_long__11	urine.with.TCDF_long__12	urine.with.TCDF_long__7	urine.with.TCDF_long__8	urine.with.TCDF_long__9	urine.with.TCDF_short__10	urine.with.TCDF_short__11	urine.with.TCDF_short__12	urine.with.TCDF_short__7	urine.with.TCDF_short__8	urine.with.TCDF_short__9	urine.with.TCDF_long__1	urine.with.TCDF_long__2	urine.with.TCDF_long__3	urine.with.TCDF_long__4	urine.with.TCDF_long__5	urine.with.TCDF_long__6	urine.with.TCDF_short__1	urine.with.TCDF_short__2	urine.with.TCDF_short__3	urine.with.TCDF_short__4	urine.with.TCDF_short__5	urine.with.TCDF_short__6
Factors	Sample source:cecal content | Genotype:GF C57BL/6J mice | Treatment:ABX water	Sample source:cecal content | Genotype:GF C57BL/6J mice | Treatment:ABX water	Sample source:cecal content | Genotype:GF C57BL/6J mice | Treatment:ABX water	Sample source:cecal content | Genotype:GF C57BL/6J mice | Treatment:Akkermansia	Sample source:cecal content | Genotype:GF C57BL/6J mice | Treatment:Akkermansia	Sample source:cecal content | Genotype:GF C57BL/6J mice | Treatment:Akkermansia	Sample source:cecal content | Genotype:GF C57BL/6J mice | Treatment:Akkermansia	Sample source:cecal content | Genotype:GF C57BL/6J mice | Treatment:Akkermansia	Sample source:cecal content | Genotype:GF C57BL/6J mice | Treatment:Akkermansia +ABX water	Sample source:cecal content | Genotype:GF C57BL/6J mice | Treatment:Akkermansia +ABX water	Sample source:cecal content | Genotype:GF C57BL/6J mice | Treatment:Akkermansia +ABX water	Sample source:cecal content | Genotype:GF C57BL/6J mice | Treatment:Akkermansia +ABX water	Sample source:cecal content | Genotype:GF C57BL/6J mice | Treatment:cecal gavage from TCDF group	Sample source:cecal content | Genotype:GF C57BL/6J mice | Treatment:cecal gavage from TCDF group	Sample source:cecal content | Genotype:GF C57BL/6J mice | Treatment:cecal gavage from TCDF group	Sample source:cecal content | Genotype:GF C57BL/6J mice | Treatment:cecal gavage from TCDF group	Sample source:cecal content | Genotype:GF C57BL/6J mice | Treatment:cecal gavage from vehicle group	Sample source:cecal content | Genotype:GF C57BL/6J mice | Treatment:cecal gavage from vehicle group	Sample source:cecal content | Genotype:GF C57BL/6J mice | Treatment:cecal gavage from vehicle group	Sample source:cecal content | Genotype:GF C57BL/6J mice | Treatment:cecal gavage from vehicle group	Sample source:cecal content | Genotype:WT C57BL/6J mice | Treatment:TCDF	Sample source:cecal content | Genotype:WT C57BL/6J mice | Treatment:TCDF	Sample source:cecal content | Genotype:WT C57BL/6J mice | Treatment:TCDF	Sample source:cecal content | Genotype:WT C57BL/6J mice | Treatment:TCDF	Sample source:cecal content | Genotype:WT C57BL/6J mice | Treatment:TCDF	Sample source:cecal content | Genotype:WT C57BL/6J mice | Treatment:TCDF	Sample source:cecal content | Genotype:WT C57BL/6J mice | Treatment:TCDF	Sample source:cecal content | Genotype:WT C57BL/6J mice | Treatment:TCDF	Sample source:cecal content | Genotype:WT C57BL/6J mice | Treatment:TCDF	Sample source:cecal content | Genotype:WT C57BL/6J mice | Treatment:TCDF	Sample source:cecal content | Genotype:WT C57BL/6J mice | Treatment:TCDF	Sample source:cecal content | Genotype:WT C57BL/6J mice | Treatment:TCDF	Sample source:cecal content | Genotype:WT C57BL/6J mice | Treatment:vehicle	Sample source:cecal content | Genotype:WT C57BL/6J mice | Treatment:vehicle	Sample source:cecal content | Genotype:WT C57BL/6J mice | Treatment:vehicle	Sample source:cecal content | Genotype:WT C57BL/6J mice | Treatment:vehicle	Sample source:cecal content | Genotype:WT C57BL/6J mice | Treatment:vehicle	Sample source:cecal content | Genotype:WT C57BL/6J mice | Treatment:vehicle	Sample source:cecal content | Genotype:WT C57BL/6J mice | Treatment:vehicle	Sample source:cecal content | Genotype:WT C57BL/6J mice | Treatment:vehicle	Sample source:cecal content | Genotype:WT C57BL/6J mice | Treatment:vehicle	Sample source:cecal content | Genotype:WT C57BL/6J mice | Treatment:vehicle	Sample source:cecal content | Genotype:WT C57BL/6J mice | Treatment:vehicle	Sample source:cecal content | Genotype:WT C57BL/6J mice | Treatment:vehicle	Sample source:liver | Genotype:Ahr-/- C57BL/6J mice | Treatment:TCDF	Sample source:liver | Genotype:Ahr-/- C57BL/6J mice | Treatment:TCDF	Sample source:liver | Genotype:Ahr-/- C57BL/6J mice | Treatment:TCDF	Sample source:liver | Genotype:Ahr-/- C57BL/6J mice | Treatment:TCDF	Sample source:liver | Genotype:Ahr-/- C57BL/6J mice | Treatment:vehicle	Sample source:liver | Genotype:Ahr-/- C57BL/6J mice | Treatment:vehicle	Sample source:liver | Genotype:Ahr-/- C57BL/6J mice | Treatment:vehicle	Sample source:liver | Genotype:Ahr-/- C57BL/6J mice | Treatment:vehicle	Sample source:liver | Genotype:GF C57BL/6J mice | Treatment:cecal gavage from TCDF group	Sample source:liver | Genotype:GF C57BL/6J mice | Treatment:cecal gavage from TCDF group	Sample source:liver | Genotype:GF C57BL/6J mice | Treatment:cecal gavage from TCDF group	Sample source:liver | Genotype:GF C57BL/6J mice | Treatment:cecal gavage from TCDF group	Sample source:liver | Genotype:GF C57BL/6J mice | Treatment:cecal gavage from TCDF group	Sample source:liver | Genotype:GF C57BL/6J mice | Treatment:cecal gavage from vehicle group	Sample source:liver | Genotype:GF C57BL/6J mice | Treatment:cecal gavage from vehicle group	Sample source:liver | Genotype:GF C57BL/6J mice | Treatment:cecal gavage from vehicle group	Sample source:liver | Genotype:GF C57BL/6J mice | Treatment:cecal gavage from vehicle group	Sample source:liver | Genotype:GF C57BL/6J mice | Treatment:cecal gavage from vehicle group	Sample source:liver | Genotype:GF C57BL/6J mice | Treatment:TCDF	Sample source:liver | Genotype:GF C57BL/6J mice | Treatment:TCDF	Sample source:liver | Genotype:GF C57BL/6J mice | Treatment:TCDF	Sample source:liver | Genotype:GF C57BL/6J mice | Treatment:TCDF	Sample source:liver | Genotype:GF C57BL/6J mice | Treatment:vehicle	Sample source:liver | Genotype:GF C57BL/6J mice | Treatment:vehicle	Sample source:liver | Genotype:GF C57BL/6J mice | Treatment:vehicle	Sample source:liver | Genotype:GF C57BL/6J mice | Treatment:vehicle	Sample source:liver | Genotype:WT C57BL/6J mice | Treatment:TCDF	Sample source:liver | Genotype:WT C57BL/6J mice | Treatment:TCDF	Sample source:liver | Genotype:WT C57BL/6J mice | Treatment:TCDF	Sample source:liver | Genotype:WT C57BL/6J mice | Treatment:TCDF	Sample source:liver | Genotype:WT C57BL/6J mice | Treatment:TCDF	Sample source:liver | Genotype:WT C57BL/6J mice | Treatment:TCDF	Sample source:liver | Genotype:WT C57BL/6J mice | Treatment:TCDF	Sample source:liver | Genotype:WT C57BL/6J mice | Treatment:TCDF	Sample source:liver | Genotype:WT C57BL/6J mice | Treatment:TCDF	Sample source:liver | Genotype:WT C57BL/6J mice | Treatment:TCDF	Sample source:liver | Genotype:WT C57BL/6J mice | Treatment:TCDF	Sample source:liver | Genotype:WT C57BL/6J mice | Treatment:TCDF	Sample source:liver | Genotype:WT C57BL/6J mice | Treatment:TCDF	Sample source:liver | Genotype:WT C57BL/6J mice | Treatment:TCDF	Sample source:liver | Genotype:WT C57BL/6J mice | Treatment:TCDF	Sample source:liver | Genotype:WT C57BL/6J mice | Treatment:TCDF	Sample source:liver | Genotype:WT C57BL/6J mice | Treatment:TCDF	Sample source:liver | Genotype:WT C57BL/6J mice | Treatment:TCDF	Sample source:liver | Genotype:WT C57BL/6J mice | Treatment:TCDF	Sample source:liver | Genotype:WT C57BL/6J mice | Treatment:TCDF	Sample source:liver | Genotype:WT C57BL/6J mice | Treatment:TCDF	Sample source:liver | Genotype:WT C57BL/6J mice | Treatment:TCDF	Sample source:liver | Genotype:WT C57BL/6J mice | Treatment:TCDF	Sample source:liver | Genotype:WT C57BL/6J mice | Treatment:TCDF	Sample source:liver | Genotype:WT C57BL/6J mice | Treatment:vehicle	Sample source:liver | Genotype:WT C57BL/6J mice | Treatment:vehicle	Sample source:liver | Genotype:WT C57BL/6J mice | Treatment:vehicle	Sample source:liver | Genotype:WT C57BL/6J mice | Treatment:vehicle	Sample source:liver | Genotype:WT C57BL/6J mice | Treatment:vehicle	Sample source:liver | Genotype:WT C57BL/6J mice | Treatment:vehicle	Sample source:liver | Genotype:WT C57BL/6J mice | Treatment:vehicle	Sample source:liver | Genotype:WT C57BL/6J mice | Treatment:vehicle	Sample source:liver | Genotype:WT C57BL/6J mice | Treatment:vehicle	Sample source:liver | Genotype:WT C57BL/6J mice | Treatment:vehicle	Sample source:liver | Genotype:WT C57BL/6J mice | Treatment:vehicle	Sample source:liver | Genotype:WT C57BL/6J mice | Treatment:vehicle	Sample source:liver | Genotype:WT C57BL/6J mice | Treatment:vehicle	Sample source:liver | Genotype:WT C57BL/6J mice | Treatment:vehicle	Sample source:liver | Genotype:WT C57BL/6J mice | Treatment:vehicle	Sample source:liver | Genotype:WT C57BL/6J mice | Treatment:vehicle	Sample source:liver | Genotype:WT C57BL/6J mice | Treatment:vehicle	Sample source:liver | Genotype:WT C57BL/6J mice | Treatment:vehicle	Sample source:liver | Genotype:WT C57BL/6J mice | Treatment:vehicle	Sample source:liver | Genotype:WT C57BL/6J mice | Treatment:vehicle	Sample source:liver | Genotype:WT C57BL/6J mice | Treatment:vehicle	Sample source:liver | Genotype:WT C57BL/6J mice | Treatment:vehicle	Sample source:liver | Genotype:WT C57BL/6J mice | Treatment:vehicle	Sample source:liver | Genotype:WT C57BL/6J mice | Treatment:vehicle	Sample source:urine | Genotype:WT C57BL/6J mice | Treatment:TCDF	Sample source:urine | Genotype:WT C57BL/6J mice | Treatment:TCDF	Sample source:urine | Genotype:WT C57BL/6J mice | Treatment:TCDF	Sample source:urine | Genotype:WT C57BL/6J mice | Treatment:TCDF	Sample source:urine | Genotype:WT C57BL/6J mice | Treatment:TCDF	Sample source:urine | Genotype:WT C57BL/6J mice | Treatment:TCDF	Sample source:urine | Genotype:WT C57BL/6J mice | Treatment:TCDF	Sample source:urine | Genotype:WT C57BL/6J mice | Treatment:TCDF	Sample source:urine | Genotype:WT C57BL/6J mice | Treatment:TCDF	Sample source:urine | Genotype:WT C57BL/6J mice | Treatment:TCDF	Sample source:urine | Genotype:WT C57BL/6J mice | Treatment:TCDF	Sample source:urine | Genotype:WT C57BL/6J mice | Treatment:TCDF	Sample source:urine | Genotype:WT C57BL/6J mice | Treatment:vehicle	Sample source:urine | Genotype:WT C57BL/6J mice | Treatment:vehicle	Sample source:urine | Genotype:WT C57BL/6J mice | Treatment:vehicle	Sample source:urine | Genotype:WT C57BL/6J mice | Treatment:vehicle	Sample source:urine | Genotype:WT C57BL/6J mice | Treatment:vehicle	Sample source:urine | Genotype:WT C57BL/6J mice | Treatment:vehicle	Sample source:urine | Genotype:WT C57BL/6J mice | Treatment:vehicle	Sample source:urine | Genotype:WT C57BL/6J mice | Treatment:vehicle	Sample source:urine | Genotype:WT C57BL/6J mice | Treatment:vehicle	Sample source:urine | Genotype:WT C57BL/6J mice | Treatment:vehicle	Sample source:urine | Genotype:WT C57BL/6J mice | Treatment:vehicle	Sample source:urine | Genotype:WT C57BL/6J mice | Treatment:vehicle	
Acetate	0.3242	0.2712	0.3681	0.6139	0.6503	0.6513	0.8700	0.9264	0.5088	0.5242	0.3444	0.3467	19.6036	12.5509	16.9418	12.6709	23.4873	25.2327	17.2582	24.8836	33.3520	36.1478	36.1800	34.0301	35.5678	28.1112	31.9056	26.1715	37.3935	37.9734	26.6431	47.4504	38.7861	41.4265	38.9737	31.4177	44.1592	43.8440	18.3657	39.4154	30.1678	24.8776	41.7682	52.3333																																																																																																		
Butyrate	0.0545	0.0237	0.0663	0.1861	0.1587	0.1855	0.0767	0.0704	0.0386	0.0860	0.0508	0.0578	4.3200	2.2527	3.0164	2.4600	5.0073	6.9873	4.2164	5.4818	7.7072	6.1657	6.5431	7.1402	7.7763	5.8007	10.6161	8.6549	11.9579	9.8158	6.3736	17.7630	11.3048	11.0566	8.7557	8.2091	9.5981	9.9659	3.6191	7.9807	6.3384	6.7676	10.5811	15.6128																																																																																																		
CE																																													3.6985	3.8762	2.6368	2.7538	3.5352	3.6760	5.1862	1.8728	2.8486	2.8185	3.7359	2.6941	2.7779	3.2074	1.9648	3.0481	2.7137	3.2914	2.9609	3.3193	2.9806	3.0572	2.8943	3.0480	2.9429	3.0425	3.6241	2.9748	3.7603	3.4581	3.9784	3.2034	3.6672	4.1514	3.1919	3.3706	3.2619	3.6500													2.7468	2.7578	3.1478	3.0261	3.3200	3.4994	2.3737	3.4097	2.5228	2.7647	2.7677	2.0986																																				
FC																																													6.7912	6.6551	7.3752	7.5820	5.9566	6.4135	5.2054	5.7618	5.0177	4.9221	5.6774	4.3720	4.5424	5.3542	3.1340	4.7060	4.9006	4.9703	5.5467	6.9827	5.8209	6.3354	6.2194	6.4845	5.5284	6.0592	5.7448	4.2790	5.1085	6.9800	5.4374	4.7119	6.6897	7.7951	6.2700	6.1370	6.0428	7.3663													3.8296	3.2064	5.0640	4.0061	4.8741	4.2676	4.6502	5.9292	5.6509	5.2965	5.3817	4.0699																																				
Glucose													39.7111	78.7489	47.7767	81.0128	30.3362	37.2586	38.4041	40.2208																																																															144.8771	135.6114	132.9753	123.8619	121.5149	126.8191	74.7347	62.4669	70.5491	69.5639	73.1273	69.7216													103.0413	101.0058	134.6952	115.9444	151.0738	141.1366	66.0290	75.7934	70.4174	63.6305	85.1961	76.7071																								
Glycogen													7.6427	29.6938	5.7603	21.7857	8.7010	21.8356	7.0724	17.4123																																																															62.0039	55.7414	58.8778	63.9624	57.3235	78.7366	33.8517	31.9829	31.3800	32.2731	32.7845	34.8502													55.7710	56.4898	57.8339	47.3301	52.4307	50.3964	19.9411	26.8574	25.9732	23.8113	39.4792	30.9388																								
LPC																																													2.4245	2.7446	2.0612	2.0935	2.2128	2.3549	2.3747	1.7339	2.4140	2.2537	2.2948	2.2569	2.3045	2.4450	1.4147	2.3425	2.2367	2.3876	2.3961	3.9295	2.6887	2.9904	3.5695	3.4827	2.9127	2.9931	2.5781	2.2980	2.2266	3.5809	2.2848	2.0621	10.1208	4.0010	12.5752	3.0153	2.8437	3.8249													3.3137	1.7139	4.0667	2.2830	2.1411	2.2588	1.9853	3.5872	3.5127	2.2291	2.8286	2.3765																																				
MUFA																																													37.6028	36.5876	34.5841	37.6333	49.4558	36.0627	36.6694	36.3620	65.7369	61.7990	65.6452	58.9014	62.9899	64.7066	43.6829	60.6050	66.7576	72.1110	49.8542	52.4781	49.2828	48.6056	36.3332	34.0695	34.2838	29.0254	41.6615	36.2156	35.7813	80.8241	45.6010	33.1852	101.8587	88.8099	97.4017	83.5828	67.4269	89.2481													33.9785	24.7699	48.0250	32.8860	34.9148	36.5301	38.2823	49.8125	35.7885	69.6435	48.3817	32.4828																																				
PC																																													21.1864	20.5744	19.9072	22.6504	22.1427	20.1337	21.3557	17.0815	21.3414	20.6393	19.0121	17.9548	18.8901	20.7702	13.5644	18.9901	19.4989	19.5686	25.6742	27.4785	26.4786	29.0257	22.2700	22.2205	21.8432	22.1776	25.0519	19.4148	24.4490	23.9003	20.5198	21.5129	16.4485	30.1492	17.9391	22.9596	26.4818	27.6631													15.7591	15.7759	21.6722	18.8879	21.5699	21.8999	18.4306	13.7272	19.7715	20.7982	18.1819	17.0655																																				
PE																																													11.4748	11.9797	10.8442	11.2054	11.2426	11.0656	10.5061	10.2449	14.8948	13.7810	13.3735	12.4993	13.5870	15.2449	9.1795	13.2794	13.4573	14.2669	12.1554	16.1800	13.8134	15.0176	16.3834	14.7439	13.2270	14.2705	16.7105	12.9067	15.2900	14.5941	12.8461	14.6472	14.7173	19.1213	16.5109	13.9384	15.7195	16.8319													11.6091	11.3209	14.6082	13.0106	12.8658	14.1438	13.3190	11.6852	14.9304	14.9501	13.7212	12.0826																																				
Propionate	0.0121	0.0169	0.0221	0.0356	0.0735	0.0632	0.0733	0.1564	0.0140	0.0246	0.0544	0.0068	5.0073	3.5018	3.2673	2.4927	4.4291	3.6982	3.3545	4.2982	9.7171	9.9729	9.8703	11.2388	9.0800	9.5309	7.2422	5.2665	6.6477	7.5135	6.4785	8.9849	10.2659	10.2747	8.3645	8.0215	9.9382	8.3328	2.8088	8.7640	5.6133	5.2810	10.4060	9.9359																																																																																																		
PUFA																																													67.8688	68.1328	55.6365	58.7630	64.2771	65.7764	67.1817	51.4002	81.6290	79.8611	91.3710	76.3563	76.1930	83.2142	56.2646	80.0960	85.3432	92.6605	69.1947	81.2059	77.1686	79.7620	69.2720	68.5498	64.1811	65.8618	76.0830	64.3633	73.6515	86.9639	78.8517	64.7177	98.2066	101.7371	93.9826	83.8544	85.6975	97.8364													59.3091	56.0892	67.3055	61.9624	61.1666	70.3282	62.4821	76.5326	70.6266	98.8132	77.1136	58.4191																																				
SFA																																													52.1734	53.2607	46.8260	51.6103	60.8269	53.3084	51.8741	47.3289	35.1379	37.1338	41.3863	38.7086	32.5757	36.4997	28.2671	37.8818	49.5622	49.9866	54.1621	58.3015	56.2018	62.5421	59.9284	57.4587	56.5631	58.3804	65.1347	52.9267	61.5132	78.4697	71.5760	57.3126	32.5403	74.4039	24.4853	58.6223	59.3250	59.4924													42.6783	47.7745	56.5741	50.3229	49.3374	59.0509	53.5673	58.8910	61.7602	82.9989	64.3044	49.8828																																				
TC																																													10.4897	10.5312	10.0121	10.3358	9.4919	10.0895	10.3917	7.6346	7.8663	7.7406	9.4133	7.0661	7.3203	8.5616	5.0987	7.7541	7.6144	8.2617	8.5076	10.3020	8.8015	9.3926	9.1137	9.5324	8.4713	9.1017	9.3690	7.2538	8.8687	8.4381	7.4158	7.9154	10.3569	11.9466	9.4619	9.5076	9.3047	11.0163													6.5765	5.9642	8.2118	7.0322	8.1941	7.7670	7.0240	9.3389	8.1737	8.0612	8.1494	6.1685																																				
TG																																													12.1730	11.5753	9.8192	10.7227	19.1981	12.0103	10.7541	14.7386											14.9345	14.4826	17.3100	16.2568	8.4011	7.8596	10.4868	8.6343																																																																								
total FA																																													157.6450	157.9810	137.0467	148.0066	174.5598	155.1475	155.7252	135.0911	182.5038	178.7939	198.4025	173.9663	171.7586	184.4204	128.2146	178.5828	201.6631	214.7581	173.2110	191.9855	182.6532	190.9097	165.5336	160.0780	155.0280	153.2675	182.8791	153.5055	170.9460	246.2577	196.0287	155.2156	232.6057	264.9508	215.8696	226.0595	212.4494	246.5769													135.9659	128.6336	171.9046	145.1712	145.4188	165.9092	154.3317	185.2361	168.1752	251.4556	189.7997	140.7847																																				
UDP-glucose													0.4854	0.3277	0.5966	0.4567	0.1395	0.3019	0.3201	0.3232																																																															0.4271	0.5129	0.4192	0.2512	0.3196	0.3490	0.4414	0.3587	0.4259	0.3642	0.4043	0.4454													0.1309	0.1554	0.1837	0.1338	0.2850	0.2305	0.4109	0.2898	0.3819	0.2699	0.2186	0.2669																								
UFA																																													105.4716	104.7203	90.2207	96.3963	113.7329	101.8391	103.8511	87.7622	116.7669	116.9949	132.7574	115.0650	108.7687	119.7139	84.5316	117.9778	134.9055	142.6471	119.0489	133.6840	126.4514	128.3677	105.6052	102.6193	98.4649	94.8872	117.7445	100.5789	109.4328	167.7880	124.4527	97.9029	200.0653	190.5469	191.3843	167.4373	153.1244	187.0845													93.2876	80.8591	115.3305	94.8483	96.0814	106.8583	100.7644	126.3451	106.4151	168.4567	125.4953	90.9019																																				
Urine hippurate																																																																																																																							0.0049	0.0038	0.0048	0.0058	0.0047	0.0054	0.0070	0.0058	0.0056	0.0057	0.0049	0.0066	0.0044	0.0074	0.0058	0.0069	0.0071	0.0063	0.0053	0.0051	0.0053	0.0065	0.0058	0.0053
Urine indoxyl sulfate																																																																																																																							0.0030	0.0021	0.0026	0.0030	0.0029	0.0029	0.0034	0.0034	0.0029	0.0026	0.0026	0.0032	0.0035	0.0038	0.0040	0.0035	0.0035	0.0038	0.0029	0.0028	0.0029	0.0028	0.0027	0.0034
NMR_METABOLITE_DATA_END
#METABOLITES
METABOLITES_START
metabolite_name	pubchem_id	inchi_key	kegg_id	other_id	other_id_type	ri	ri_type	moverz_quant	
Acetate	175								
Butyrate	264								
CE	23974								
FC	18218231								
Glucose	5793								
Glycogen	439177								
LPC									
MUFA									
PC									
PE									
Propionate	104745								
PUFA									
SFA	5388925								
TC	23957								
TG	2723601								
total FA									
UDP-glucose	8629								
UFA									
Urine hippurate									
Urine indoxyl sulfate									
METABOLITES_END
#END