#METABOLOMICS WORKBENCH Linyan_20240328_112326 DATATRACK_ID:4748 STUDY_ID:ST003155 ANALYSIS_ID:AN005176 PROJECT_ID:PR001961
VERSION             	1
CREATED_ON             	April 1, 2024, 9:21 pm
#PROJECT
PR:PROJECT_TITLE                 	NMR-based metabolomics combined with metabolic pathway analysis reveals
PR:PROJECT_TITLE                 	metabolic heterogeneity of colorectal cancer tissue at different anatomical
PR:PROJECT_TITLE                 	locations and stages
PR:PROJECT_SUMMARY               	Colorectal cancer (CRC) still remains the leading cause of cancer death
PR:PROJECT_SUMMARY               	worldwide. This study aimed to profile the metabolic differences of colorectal
PR:PROJECT_SUMMARY               	cancer tissues (CCT) at different stages and sites, as compared with their
PR:PROJECT_SUMMARY               	adjacent noncancerous tissues (ANT), to investigate the temporal and spatial
PR:PROJECT_SUMMARY               	heterogeneities of metabolic characterization. Our NMR-based metabolomics
PR:PROJECT_SUMMARY               	fingerprinting revealed that many of the metabolite levels were significantly
PR:PROJECT_SUMMARY               	altered in CCT compared to ANT and esophageal cancer tissues, indicating
PR:PROJECT_SUMMARY               	deregulations of glucose metabolism, one-carbon metabolism, glutamine
PR:PROJECT_SUMMARY               	metabolism, amino acid metabolism, fatty acid metabolism, TCA cycle, choline
PR:PROJECT_SUMMARY               	metabolism, etc. A total of five biomarker metabolites, including glucose,
PR:PROJECT_SUMMARY               	glutamate, alanine, valine and histidine, were identified to distinguish between
PR:PROJECT_SUMMARY               	early and advanced stages of CCT. Metabolites that distinguish the different
PR:PROJECT_SUMMARY               	anatomical sites of CCT include glucose, glycerol, glutamine, inositol,
PR:PROJECT_SUMMARY               	succinate, and citrate. Those significant metabolic differences in CRC tissues
PR:PROJECT_SUMMARY               	at different pathological stages and sites suggested temporal and spatial
PR:PROJECT_SUMMARY               	heterogeneities of metabolic characterization in CCT, providing a metabolic
PR:PROJECT_SUMMARY               	foundation for further study on biofluid metabolism in CRC early detection.
PR:INSTITUTE                     	Radiology Department, Second Affiliated Hospital, Shantou University Medical
PR:INSTITUTE                     	College, Shantou
PR:LAST_NAME                     	Lin
PR:FIRST_NAME                    	Yan
PR:ADDRESS                       	No. 69, Dongxia North Road, Shantou, Guangdong, China, Shantou, Guangdong,
PR:ADDRESS                       	China, 515041, China
PR:EMAIL                         	994809889@qq.com
PR:PHONE                         	+86 18823992148
#STUDY
ST:STUDY_TITLE                   	NMR-based metabolomics combined with metabolic pathway analysis reveals
ST:STUDY_TITLE                   	metabolic heterogeneity of colorectal cancer tissue at different anatomical
ST:STUDY_TITLE                   	locations and stages
ST:STUDY_SUMMARY                 	Colorectal cancer (CRC) still remains the leading cause of cancer death
ST:STUDY_SUMMARY                 	worldwide. This study aimed to profile the metabolic differences of colorectal
ST:STUDY_SUMMARY                 	cancer tissues (CCT) at different stages and sites, as compared with their
ST:STUDY_SUMMARY                 	adjacent noncancerous tissues (ANT), to investigate the temporal and spatial
ST:STUDY_SUMMARY                 	heterogeneities of metabolic characterization. Our NMR-based metabolomics
ST:STUDY_SUMMARY                 	fingerprinting revealed that many of the metabolite levels were significantly
ST:STUDY_SUMMARY                 	altered in CCT compared to ANT and esophageal cancer tissues, indicating
ST:STUDY_SUMMARY                 	deregulations of glucose metabolism, one-carbon metabolism, glutamine
ST:STUDY_SUMMARY                 	metabolism, amino acid metabolism, fatty acid metabolism, TCA cycle, choline
ST:STUDY_SUMMARY                 	metabolism, etc. A total of five biomarker metabolites, including glucose,
ST:STUDY_SUMMARY                 	glutamate, alanine, valine and histidine, were identified to distinguish between
ST:STUDY_SUMMARY                 	early and advanced stages of CCT. Metabolites that distinguish the different
ST:STUDY_SUMMARY                 	anatomical sites of CCT include glucose, glycerol, glutamine, inositol,
ST:STUDY_SUMMARY                 	succinate, and citrate. Those significant metabolic differences in CRC tissues
ST:STUDY_SUMMARY                 	at different pathological stages and sites suggested temporal and spatial
ST:STUDY_SUMMARY                 	heterogeneities of metabolic characterization in CCT, providing a metabolic
ST:STUDY_SUMMARY                 	foundation for further study on biofluid metabolism in CRC early detection.
ST:INSTITUTE                     	Radiology Department, Second Affiliated Hospital, Shantou University Medical
ST:INSTITUTE                     	College, Shantou
ST:LAST_NAME                     	Lin
ST:FIRST_NAME                    	Yan
ST:ADDRESS                       	No. 69, Dongxia North Road, Shantou, Guangdong, China, Shantou, Guangdong,
ST:ADDRESS                       	China, 515041, China
ST:EMAIL                         	994809889@qq.com
ST:PHONE                         	+86 18823992148
#SUBJECT
SU:SUBJECT_TYPE                  	Human
SU:SUBJECT_SPECIES               	Homo sapiens
SU:TAXONOMY_ID                   	9606
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data
SUBJECT_SAMPLE_FACTORS           	C_C0_1	CRC-tissue-C0.1.fid#	Sample source:Colorectal tissue | Factor:early CRC | Factor:CRC	RAW_FILE_NAME(Raw file name)=CRC-tissue-C0.1.fid
SUBJECT_SAMPLE_FACTORS           	C_C0_2	CRC-tissue-C0.2.fid#	Sample source:Colorectal tissue | Factor:early CRC | Factor:RCC	RAW_FILE_NAME(Raw file name)=CRC-tissue-C0.2.fid
SUBJECT_SAMPLE_FACTORS           	C_C0_3	CRC-tissue-C0.3.fid#	Sample source:Colorectal tissue | Factor:early CRC | Factor:rectum	RAW_FILE_NAME(Raw file name)=CRC-tissue-C0.3.fid
SUBJECT_SAMPLE_FACTORS           	C_C0_4	CRC-tissue-C0.4.fid#	Sample source:Colorectal tissue | Factor:early CRC | Factor:LCC	RAW_FILE_NAME(Raw file name)=CRC-tissue-C0.4.fid
SUBJECT_SAMPLE_FACTORS           	C_C0_5	CRC-tissue-C0.5.fid#	Sample source:Colorectal tissue | Factor:early CRC | Factor:rectum	RAW_FILE_NAME(Raw file name)=CRC-tissue-C0.5.fid
SUBJECT_SAMPLE_FACTORS           	C_C0_6	CRC-tissue-C0.6.fid#	Sample source:Colorectal tissue | Factor:early CRC | Factor:LCC	RAW_FILE_NAME(Raw file name)=CRC-tissue-C0.6.fid
SUBJECT_SAMPLE_FACTORS           	C_C0_7	CRC-tissue-C0.7.fid#	Sample source:Colorectal tissue | Factor:early CRC | Factor:rectum	RAW_FILE_NAME(Raw file name)=CRC-tissue-C0.7.fid
SUBJECT_SAMPLE_FACTORS           	C_C0_8	CRC-tissue-C0.8.fid#	Sample source:Colorectal tissue | Factor:early CRC | Factor:rectum	RAW_FILE_NAME(Raw file name)=CRC-tissue-C0.8.fid
SUBJECT_SAMPLE_FACTORS           	C_C0_9	CRC-tissue-C0.9.fid#	Sample source:Colorectal tissue | Factor:early CRC | Factor:RCC	RAW_FILE_NAME(Raw file name)=CRC-tissue-C0.9.fid
SUBJECT_SAMPLE_FACTORS           	C_C0_10	CRC-tissue-C0.10.fid#	Sample source:Colorectal tissue | Factor:early CRC | Factor:RCC	RAW_FILE_NAME(Raw file name)=CRC-tissue-C0.10.fid
SUBJECT_SAMPLE_FACTORS           	C_C0_11	CRC-tissue-C0.11.fid#	Sample source:Colorectal tissue | Factor:early CRC | Factor:LCC	RAW_FILE_NAME(Raw file name)=CRC-tissue-C0.11.fid
SUBJECT_SAMPLE_FACTORS           	C_C0_12	CRC-tissue-C0.12.fid#	Sample source:Colorectal tissue | Factor:early CRC | Factor:LCC	RAW_FILE_NAME(Raw file name)=CRC-tissue-C0.12.fid
SUBJECT_SAMPLE_FACTORS           	C_C0_13	CRC-tissue-C0.13.fid#	Sample source:Colorectal tissue | Factor:early CRC | Factor:LCC	RAW_FILE_NAME(Raw file name)=CRC-tissue-C0.13.fid
SUBJECT_SAMPLE_FACTORS           	C_C0_14	CRC-tissue-C0.14.fid#	Sample source:Colorectal tissue | Factor:early CRC | Factor:colon	RAW_FILE_NAME(Raw file name)=CRC-tissue-C0.14.fid
SUBJECT_SAMPLE_FACTORS           	C_C0_15	CRC-tissue-C0.15.fid#	Sample source:Colorectal tissue | Factor:early CRC | Factor:colon	RAW_FILE_NAME(Raw file name)=CRC-tissue-C0.15.fid
SUBJECT_SAMPLE_FACTORS           	C_C0_16	CRC-tissue-C0.16.fid#	Sample source:Colorectal tissue | Factor:early CRC | Factor:RCC	RAW_FILE_NAME(Raw file name)=CRC-tissue-C0.16.fid
SUBJECT_SAMPLE_FACTORS           	C_C0_17	CRC-tissue-C0.17.fid#	Sample source:Colorectal tissue | Factor:early CRC | Factor:rectum	RAW_FILE_NAME(Raw file name)=CRC-tissue-C0.17.fid
SUBJECT_SAMPLE_FACTORS           	C_C0_18	CRC-tissue-C0.18.fid#	Sample source:Colorectal tissue | Factor:early CRC | Factor:RCC	RAW_FILE_NAME(Raw file name)=CRC-tissue-C0.18.fid
SUBJECT_SAMPLE_FACTORS           	C_C0_19	CRC-tissue-C0.19.fid#	Sample source:Colorectal tissue | Factor:early CRC | Factor:LCC	RAW_FILE_NAME(Raw file name)=CRC-tissue-C0.19.fid
SUBJECT_SAMPLE_FACTORS           	C_C0_20	CRC-tissue-C0.20.fid#	Sample source:Colorectal tissue | Factor:early CRC | Factor:rectum	RAW_FILE_NAME(Raw file name)=CRC-tissue-C0.20.fid
SUBJECT_SAMPLE_FACTORS           	C_C0_21	CRC-tissue-C0.21.fid#	Sample source:Colorectal tissue | Factor:early CRC | Factor:RCC	RAW_FILE_NAME(Raw file name)=CRC-tissue-C0.21.fid
SUBJECT_SAMPLE_FACTORS           	C_C0_22	CRC-tissue-C0.22.fid#	Sample source:Colorectal tissue | Factor:early CRC | Factor:LCC	RAW_FILE_NAME(Raw file name)=CRC-tissue-C0.22.fid
SUBJECT_SAMPLE_FACTORS           	C_C0_23	CRC-tissue-C0.23.fid#	Sample source:Colorectal tissue | Factor:early CRC | Factor:LCC	RAW_FILE_NAME(Raw file name)=CRC-tissue-C0.23.fid
SUBJECT_SAMPLE_FACTORS           	C_C0_24	CRC-tissue-C0.24.fid#	Sample source:Colorectal tissue | Factor:early CRC | Factor:LCC	RAW_FILE_NAME(Raw file name)=CRC-tissue-C0.24.fid
SUBJECT_SAMPLE_FACTORS           	C_C0_25	CRC-tissue-C0.25.fid#	Sample source:Colorectal tissue | Factor:early CRC | Factor:LCC	RAW_FILE_NAME(Raw file name)=CRC-tissue-C0.25.fid
SUBJECT_SAMPLE_FACTORS           	C_C0_26	CRC-tissue-C0.26.fid#	Sample source:Colorectal tissue | Factor:advanced CRC | Factor:rectum	RAW_FILE_NAME(Raw file name)=CRC-tissue-C0.26.fid
SUBJECT_SAMPLE_FACTORS           	C_C0_27	CRC-tissue-C0.27.fid#	Sample source:Colorectal tissue | Factor:advanced CRC | Factor:RCC	RAW_FILE_NAME(Raw file name)=CRC-tissue-C0.27.fid
SUBJECT_SAMPLE_FACTORS           	C_C0_28	CRC-tissue-C0.28.fid#	Sample source:Colorectal tissue | Factor:advanced CRC | Factor:rectum	RAW_FILE_NAME(Raw file name)=CRC-tissue-C0.28.fid
SUBJECT_SAMPLE_FACTORS           	C_C0_29	CRC-tissue-C0.29.fid#	Sample source:Colorectal tissue | Factor:advanced CRC | Factor:RCC	RAW_FILE_NAME(Raw file name)=CRC-tissue-C0.29.fid
SUBJECT_SAMPLE_FACTORS           	C_C0_30	CRC-tissue-C0.30.fid#	Sample source:Colorectal tissue | Factor:advanced CRC | Factor:rectum	RAW_FILE_NAME(Raw file name)=CRC-tissue-C0.30.fid
SUBJECT_SAMPLE_FACTORS           	C_C0_31	CRC-tissue-C0.31.fid#	Sample source:Colorectal tissue | Factor:advanced CRC | Factor:LCC	RAW_FILE_NAME(Raw file name)=CRC-tissue-C0.31.fid
SUBJECT_SAMPLE_FACTORS           	C_C0_32	CRC-tissue-C0.32.fid#	Sample source:Colorectal tissue | Factor:advanced CRC | Factor:rectum	RAW_FILE_NAME(Raw file name)=CRC-tissue-C0.32.fid
SUBJECT_SAMPLE_FACTORS           	C_C0_33	CRC-tissue-C0.33.fid#	Sample source:Colorectal tissue | Factor:advanced CRC | Factor:rectum	RAW_FILE_NAME(Raw file name)=CRC-tissue-C0.33.fid
SUBJECT_SAMPLE_FACTORS           	C_C0_34	CRC-tissue-C0.34.fid#	Sample source:Colorectal tissue | Factor:advanced CRC | Factor:rectum	RAW_FILE_NAME(Raw file name)=CRC-tissue-C0.34.fid
SUBJECT_SAMPLE_FACTORS           	C_C0_35	CRC-tissue-C0.35.fid#	Sample source:Colorectal tissue | Factor:advanced CRC | Factor:rectum	RAW_FILE_NAME(Raw file name)=CRC-tissue-C0.35.fid
SUBJECT_SAMPLE_FACTORS           	C_C0_36	CRC-tissue-C0.36.fid#	Sample source:Colorectal tissue | Factor:advanced CRC | Factor:LCC	RAW_FILE_NAME(Raw file name)=CRC-tissue-C0.36.fid
SUBJECT_SAMPLE_FACTORS           	C_C0_37	CRC-tissue-C0.37.fid#	Sample source:Colorectal tissue | Factor:advanced CRC | Factor:RCC	RAW_FILE_NAME(Raw file name)=CRC-tissue-C0.37.fid
SUBJECT_SAMPLE_FACTORS           	C_C0_38	CRC-tissue-C0.38.fid#	Sample source:Colorectal tissue | Factor:advanced CRC | Factor:LCC	RAW_FILE_NAME(Raw file name)=CRC-tissue-C0.38.fid
SUBJECT_SAMPLE_FACTORS           	C_C0_39	CRC-tissue-C0.39.fid#	Sample source:Colorectal tissue | Factor:advanced CRC | Factor:LCC	RAW_FILE_NAME(Raw file name)=CRC-tissue-C0.39.fid
SUBJECT_SAMPLE_FACTORS           	C_C0_40	CRC-tissue-C0.40.fid#	Sample source:Colorectal tissue | Factor:advanced CRC | Factor:CRC	RAW_FILE_NAME(Raw file name)=CRC-tissue-C0.40.fid
SUBJECT_SAMPLE_FACTORS           	C_C0_41	CRC-tissue-C0.41.fid#	Sample source:Colorectal tissue | Factor:advanced CRC | Factor:rectum	RAW_FILE_NAME(Raw file name)=CRC-tissue-C0.41.fid
SUBJECT_SAMPLE_FACTORS           	C_C0_42	CRC-tissue-C0.42.fid#	Sample source:Colorectal tissue | Factor:advanced CRC | Factor:rectum	RAW_FILE_NAME(Raw file name)=CRC-tissue-C0.42.fid
SUBJECT_SAMPLE_FACTORS           	C_C0_43	CRC-tissue-C0.43.fid#	Sample source:Colorectal tissue | Factor:advanced CRC | Factor:rectum	RAW_FILE_NAME(Raw file name)=CRC-tissue-C0.43.fid
SUBJECT_SAMPLE_FACTORS           	C_C0_44	CRC-tissue-C0.44.fid#	Sample source:Colorectal tissue | Factor:advanced CRC | Factor:LCC	RAW_FILE_NAME(Raw file name)=CRC-tissue-C0.44.fid
SUBJECT_SAMPLE_FACTORS           	C_C0_45	CRC-tissue-C0.45.fid#	Sample source:Colorectal tissue | Factor:advanced CRC | Factor:CRC	RAW_FILE_NAME(Raw file name)=CRC-tissue-C0.45.fid
SUBJECT_SAMPLE_FACTORS           	C_C0_46	CRC-tissue-C0.46.fid#	Sample source:Colorectal tissue | Factor:advanced CRC | Factor:rectum	RAW_FILE_NAME(Raw file name)=CRC-tissue-C0.46.fid
SUBJECT_SAMPLE_FACTORS           	C_C0_47	CRC-tissue-C0.47.fid#	Sample source:Colorectal tissue | Factor:advanced CRC | Factor:RCC	RAW_FILE_NAME(Raw file name)=CRC-tissue-C0.47.fid
SUBJECT_SAMPLE_FACTORS           	C_C0_48	CRC-tissue-C0.48.fid#	Sample source:Colorectal tissue | Factor:advanced CRC | Factor:rectum	RAW_FILE_NAME(Raw file name)=CRC-tissue-C0.48.fid
SUBJECT_SAMPLE_FACTORS           	C_C0_49	CRC-tissue-C0.49.fid#	Sample source:Colorectal tissue | Factor:advanced CRC | Factor:colon	RAW_FILE_NAME(Raw file name)=CRC-tissue-C0.49.fid
SUBJECT_SAMPLE_FACTORS           	C_C0_50	CRC-tissue-C0.50.fid#	Sample source:Colorectal tissue | Factor:advanced CRC | Factor:rectum	RAW_FILE_NAME(Raw file name)=CRC-tissue-C0.50.fid
SUBJECT_SAMPLE_FACTORS           	C_C0_51	CRC-tissue-C0.51.fid#	Sample source:Colorectal tissue | Factor:advanced CRC | Factor:RCC	RAW_FILE_NAME(Raw file name)=CRC-tissue-C0.51.fid
SUBJECT_SAMPLE_FACTORS           	C_C0_52	CRC-tissue-C0.52.fid#	Sample source:Colorectal tissue | Factor:advanced CRC | Factor:RCC	RAW_FILE_NAME(Raw file name)=CRC-tissue-C0.52.fid
SUBJECT_SAMPLE_FACTORS           	C_P5_1	CRC-tissue-P5.105.fid#	Sample source:Colorectal tissue | Factor:early para-CRC | Factor:CRC	RAW_FILE_NAME(Raw file name)=CRC-tissue-P5.105.fid
SUBJECT_SAMPLE_FACTORS           	C_P5_2	CRC-tissue-P5.106.fid#	Sample source:Colorectal tissue | Factor:early para-CRC | Factor:RCC	RAW_FILE_NAME(Raw file name)=CRC-tissue-P5.106.fid
SUBJECT_SAMPLE_FACTORS           	C_P5_3	CRC-tissue-P5.107.fid#	Sample source:Colorectal tissue | Factor:early para-CRC | Factor:rectum	RAW_FILE_NAME(Raw file name)=CRC-tissue-P5.107.fid
SUBJECT_SAMPLE_FACTORS           	C_P5_4	CRC-tissue-P5.108.fid#	Sample source:Colorectal tissue | Factor:early para-CRC | Factor:LCC	RAW_FILE_NAME(Raw file name)=CRC-tissue-P5.108.fid
SUBJECT_SAMPLE_FACTORS           	C_P5_5	CRC-tissue-P5.109.fid#	Sample source:Colorectal tissue | Factor:early para-CRC | Factor:rectum	RAW_FILE_NAME(Raw file name)=CRC-tissue-P5.109.fid
SUBJECT_SAMPLE_FACTORS           	C_P5_6	CRC-tissue-P5.110.fid#	Sample source:Colorectal tissue | Factor:early para-CRC | Factor:LCC	RAW_FILE_NAME(Raw file name)=CRC-tissue-P5.110.fid
SUBJECT_SAMPLE_FACTORS           	C_P5_7	CRC-tissue-P5.111.fid#	Sample source:Colorectal tissue | Factor:early para-CRC | Factor:rectum	RAW_FILE_NAME(Raw file name)=CRC-tissue-P5.111.fid
SUBJECT_SAMPLE_FACTORS           	C_P5_8	CRC-tissue-P5.112.fid#	Sample source:Colorectal tissue | Factor:early para-CRC | Factor:rectum	RAW_FILE_NAME(Raw file name)=CRC-tissue-P5.112.fid
SUBJECT_SAMPLE_FACTORS           	C_P5_9	CRC-tissue-P5.113.fid#	Sample source:Colorectal tissue | Factor:early para-CRC | Factor:RCC	RAW_FILE_NAME(Raw file name)=CRC-tissue-P5.113.fid
SUBJECT_SAMPLE_FACTORS           	C_P5_10	CRC-tissue-P5.114.fid#	Sample source:Colorectal tissue | Factor:early para-CRC | Factor:RCC	RAW_FILE_NAME(Raw file name)=CRC-tissue-P5.114.fid
SUBJECT_SAMPLE_FACTORS           	C_P5_11	CRC-tissue-P5.115.fid#	Sample source:Colorectal tissue | Factor:early para-CRC | Factor:LCC	RAW_FILE_NAME(Raw file name)=CRC-tissue-P5.115.fid
SUBJECT_SAMPLE_FACTORS           	C_P5_12	CRC-tissue-P5.116.fid#	Sample source:Colorectal tissue | Factor:early para-CRC | Factor:LCC	RAW_FILE_NAME(Raw file name)=CRC-tissue-P5.116.fid
SUBJECT_SAMPLE_FACTORS           	C_P5_13	CRC-tissue-P5.117.fid#	Sample source:Colorectal tissue | Factor:early para-CRC | Factor:LCC	RAW_FILE_NAME(Raw file name)=CRC-tissue-P5.117.fid
SUBJECT_SAMPLE_FACTORS           	C_P5_14	CRC-tissue-P5.118.fid#	Sample source:Colorectal tissue | Factor:early para-CRC | Factor:CRC	RAW_FILE_NAME(Raw file name)=CRC-tissue-P5.118.fid
SUBJECT_SAMPLE_FACTORS           	C_P5_15	CRC-tissue-P5.119.fid#	Sample source:Colorectal tissue | Factor:early para-CRC | Factor:LCC	RAW_FILE_NAME(Raw file name)=CRC-tissue-P5.119.fid
SUBJECT_SAMPLE_FACTORS           	C_P5_16	CRC-tissue-P5.120.fid#	Sample source:Colorectal tissue | Factor:early para-CRC | Factor:RCC	RAW_FILE_NAME(Raw file name)=CRC-tissue-P5.120.fid
SUBJECT_SAMPLE_FACTORS           	C_P5_17	CRC-tissue-P5.121.fid#	Sample source:Colorectal tissue | Factor:early para-CRC | Factor:rectum	RAW_FILE_NAME(Raw file name)=CRC-tissue-P5.121.fid
SUBJECT_SAMPLE_FACTORS           	C_P5_18	CRC-tissue-P5.122.fid#	Sample source:Colorectal tissue | Factor:early para-CRC | Factor:RCC	RAW_FILE_NAME(Raw file name)=CRC-tissue-P5.122.fid
SUBJECT_SAMPLE_FACTORS           	C_P5_19	CRC-tissue-P5.123.fid#	Sample source:Colorectal tissue | Factor:early para-CRC | Factor:LCC	RAW_FILE_NAME(Raw file name)=CRC-tissue-P5.123.fid
SUBJECT_SAMPLE_FACTORS           	C_P5_20	CRC-tissue-P5.124.fid#	Sample source:Colorectal tissue | Factor:early para-CRC | Factor:rectum	RAW_FILE_NAME(Raw file name)=CRC-tissue-P5.124.fid
SUBJECT_SAMPLE_FACTORS           	C_P5_21	CRC-tissue-P5.125.fid#	Sample source:Colorectal tissue | Factor:early para-CRC | Factor:RCC	RAW_FILE_NAME(Raw file name)=CRC-tissue-P5.125.fid
SUBJECT_SAMPLE_FACTORS           	C_P5_22	CRC-tissue-P5.126.fid#	Sample source:Colorectal tissue | Factor:early para-CRC | Factor:LCC	RAW_FILE_NAME(Raw file name)=CRC-tissue-P5.126.fid
SUBJECT_SAMPLE_FACTORS           	C_P5_23	CRC-tissue-P5.127.fid#	Sample source:Colorectal tissue | Factor:early para-CRC | Factor:LCC	RAW_FILE_NAME(Raw file name)=CRC-tissue-P5.127.fid
SUBJECT_SAMPLE_FACTORS           	C_P5_24	CRC-tissue-P5.128.fid#	Sample source:Colorectal tissue | Factor:early para-CRC | Factor:LCC	RAW_FILE_NAME(Raw file name)=CRC-tissue-P5.128.fid
SUBJECT_SAMPLE_FACTORS           	C_P5_25	CRC-tissue-P5.129.fid#	Sample source:Colorectal tissue | Factor:early para-CRC | Factor:LCC	RAW_FILE_NAME(Raw file name)=CRC-tissue-P5.129.fid
SUBJECT_SAMPLE_FACTORS           	C_P5_26	CRC-tissue-P5.130.fid#	Sample source:Colorectal tissue | Factor:advanced para-CRC | Factor:rectum	RAW_FILE_NAME(Raw file name)=CRC-tissue-P5.130.fid
SUBJECT_SAMPLE_FACTORS           	C_P5_27	CRC-tissue-P5.131.fid#	Sample source:Colorectal tissue | Factor:advanced para-CRC | Factor:RCC	RAW_FILE_NAME(Raw file name)=CRC-tissue-P5.131.fid
SUBJECT_SAMPLE_FACTORS           	C_P5_28	CRC-tissue-P5.132.fid#	Sample source:Colorectal tissue | Factor:advanced para-CRC | Factor:rectum	RAW_FILE_NAME(Raw file name)=CRC-tissue-P5.132.fid
SUBJECT_SAMPLE_FACTORS           	C_P5_29	CRC-tissue-P5.133.fid#	Sample source:Colorectal tissue | Factor:advanced para-CRC | Factor:RCC	RAW_FILE_NAME(Raw file name)=CRC-tissue-P5.133.fid
SUBJECT_SAMPLE_FACTORS           	C_P5_30	CRC-tissue-P5.134.fid#	Sample source:Colorectal tissue | Factor:advanced para-CRC | Factor:rectum	RAW_FILE_NAME(Raw file name)=CRC-tissue-P5.134.fid
SUBJECT_SAMPLE_FACTORS           	C_P5_31	CRC-tissue-P5.135.fid#	Sample source:Colorectal tissue | Factor:advanced para-CRC | Factor:rectum	RAW_FILE_NAME(Raw file name)=CRC-tissue-P5.135.fid
SUBJECT_SAMPLE_FACTORS           	C_P5_33	CRC-tissue-P5.136.fid#	Sample source:Colorectal tissue | Factor:advanced para-CRC | Factor:rectum	RAW_FILE_NAME(Raw file name)=CRC-tissue-P5.136.fid
SUBJECT_SAMPLE_FACTORS           	C_P5_34	CRC-tissue-P5.137.fid#	Sample source:Colorectal tissue | Factor:advanced para-CRC | Factor:rectum	RAW_FILE_NAME(Raw file name)=CRC-tissue-P5.137.fid
SUBJECT_SAMPLE_FACTORS           	C_P5_35	CRC-tissue-P5.138.fid#	Sample source:Colorectal tissue | Factor:advanced para-CRC | Factor:rectum	RAW_FILE_NAME(Raw file name)=CRC-tissue-P5.138.fid
SUBJECT_SAMPLE_FACTORS           	C_P5_36	CRC-tissue-P5.139.fid#	Sample source:Colorectal tissue | Factor:advanced para-CRC | Factor:LCC	RAW_FILE_NAME(Raw file name)=CRC-tissue-P5.139.fid
SUBJECT_SAMPLE_FACTORS           	C_P5_37	CRC-tissue-P5.140.fid#	Sample source:Colorectal tissue | Factor:advanced para-CRC | Factor:RCC	RAW_FILE_NAME(Raw file name)=CRC-tissue-P5.140.fid
SUBJECT_SAMPLE_FACTORS           	C_P5_38	CRC-tissue-P5.141.fid#	Sample source:Colorectal tissue | Factor:advanced para-CRC | Factor:LCC	RAW_FILE_NAME(Raw file name)=CRC-tissue-P5.141.fid
SUBJECT_SAMPLE_FACTORS           	C_P5_39	CRC-tissue-P5.142.fid#	Sample source:Colorectal tissue | Factor:advanced para-CRC | Factor:LCC	RAW_FILE_NAME(Raw file name)=CRC-tissue-P5.142.fid
SUBJECT_SAMPLE_FACTORS           	C_P5_40	CRC-tissue-P5.143.fid#	Sample source:Colorectal tissue | Factor:advanced para-CRC | Factor:CRC	RAW_FILE_NAME(Raw file name)=CRC-tissue-P5.143.fid
SUBJECT_SAMPLE_FACTORS           	C_P5_41	CRC-tissue-P5.144.fid#	Sample source:Colorectal tissue | Factor:advanced para-CRC | Factor:rectum	RAW_FILE_NAME(Raw file name)=CRC-tissue-P5.144.fid
SUBJECT_SAMPLE_FACTORS           	C_P5_42	CRC-tissue-P5.145.fid#	Sample source:Colorectal tissue | Factor:advanced para-CRC | Factor:rectum	RAW_FILE_NAME(Raw file name)=CRC-tissue-P5.145.fid
SUBJECT_SAMPLE_FACTORS           	C_P5_43	CRC-tissue-P5.146.fid#	Sample source:Colorectal tissue | Factor:advanced para-CRC | Factor:LCC	RAW_FILE_NAME(Raw file name)=CRC-tissue-P5.146.fid
SUBJECT_SAMPLE_FACTORS           	C_P5_45	CRC-tissue-P5.147.fid#	Sample source:Colorectal tissue | Factor:advanced para-CRC | Factor:CRC	RAW_FILE_NAME(Raw file name)=CRC-tissue-P5.147.fid
SUBJECT_SAMPLE_FACTORS           	C_P5_46	CRC-tissue-P5.148.fid#	Sample source:Colorectal tissue | Factor:advanced para-CRC | Factor:rectum	RAW_FILE_NAME(Raw file name)=CRC-tissue-P5.148.fid
SUBJECT_SAMPLE_FACTORS           	C_P5_47	CRC-tissue-P5.149.fid#	Sample source:Colorectal tissue | Factor:advanced para-CRC | Factor:RCC	RAW_FILE_NAME(Raw file name)=CRC-tissue-P5.149.fid
SUBJECT_SAMPLE_FACTORS           	C_P5_48	CRC-tissue-P5.150.fid#	Sample source:Colorectal tissue | Factor:advanced para-CRC | Factor:rectum	RAW_FILE_NAME(Raw file name)=CRC-tissue-P5.150.fid
SUBJECT_SAMPLE_FACTORS           	C_P5_49	CRC-tissue-P5.151.fid#	Sample source:Colorectal tissue | Factor:advanced para-CRC | Factor:LCC	RAW_FILE_NAME(Raw file name)=CRC-tissue-P5.151.fid
SUBJECT_SAMPLE_FACTORS           	C_P5_50	CRC-tissue-P5.152.fid#	Sample source:Colorectal tissue | Factor:advanced para-CRC | Factor:rectum	RAW_FILE_NAME(Raw file name)=CRC-tissue-P5.152.fid
SUBJECT_SAMPLE_FACTORS           	C_P5_51	CRC-tissue-P5.153.fid#	Sample source:Colorectal tissue | Factor:advanced para-CRC | Factor:RCC	RAW_FILE_NAME(Raw file name)=CRC-tissue-P5.153.fid
SUBJECT_SAMPLE_FACTORS           	C_P5_52	CRC-tissue-P5.154.fid#	Sample source:Colorectal tissue | Factor:advanced para-CRC | Factor:RCC	RAW_FILE_NAME(Raw file name)=CRC-tissue-P5.154.fid
#COLLECTION
CO:COLLECTION_SUMMARY            	Resected colorectal cancer tissues and their corresponding non-cancerous tissues
CO:COLLECTION_SUMMARY            	were frozen in liquid nitrogen for 10s before being transferred to cryogenic
CO:COLLECTION_SUMMARY            	vials and stored at −80°C until metabolite extraction.
CO:SAMPLE_TYPE                   	colorectal tissue
CO:STORAGE_CONDITIONS            	-80℃
#TREATMENT
TR:TREATMENT_SUMMARY             	None
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	Frozen tissue samples (approximately 300 mg) were thawed at 25°C, then
SP:SAMPLEPREP_SUMMARY            	sectioned and homogenized at 16,000 rpm for 80 seconds in a solution consisting
SP:SAMPLEPREP_SUMMARY            	of 0.6 mL distilled water and 1.2 mL methanol. Subsequently, chloroform (1.2 mL)
SP:SAMPLEPREP_SUMMARY            	and distilled water (1.2 mL) were added and the sample was vortexed for 60
SP:SAMPLEPREP_SUMMARY            	seconds. Finally, samples were incubated on ice for 15 mins, followed by
SP:SAMPLEPREP_SUMMARY            	centrifugation at 2000 rpm for 5 mins. The resulting supernatant was washed with
SP:SAMPLEPREP_SUMMARY            	nitrogen and evaporated before incubating under vacuum for at least 18 hours.
SP:SAMPLEPREP_SUMMARY            	The subsequent lyophilized powder was dissolved in 550 μL PBS/D2O buffer (0.1
SP:SAMPLEPREP_SUMMARY            	M, pH 7.4) and 50 μL TSP/D2O stock solution, and after centrifugation at 10,000
SP:SAMPLEPREP_SUMMARY            	rpm for 5 mins,500 μL of the supernatant was transferred to an NMR tube (5 mm
SP:SAMPLEPREP_SUMMARY            	size) for 1H NMR spectroscopy analysis.
#ANALYSIS
AN:ANALYSIS_TYPE                 	NMR
#NMR
NM:INSTRUMENT_NAME               	Bruker Avance III
NM:INSTRUMENT_TYPE               	FT-NMR
NM:NMR_EXPERIMENT_TYPE           	1D-1H
NM:SPECTROMETER_FREQUENCY        	600 MHz
NM:NMR_RESULTS_FILE               	ST003155_AN005176_Results.txt	UNITS:peak area
#END