#METABOLOMICS WORKBENCH aragron135_20240513_100556 DATATRACK_ID:4838 STUDY_ID:ST003212 ANALYSIS_ID:AN005268 PROJECT_ID:PR002003 VERSION 1 CREATED_ON May 15, 2024, 10:27 am #PROJECT PR:PROJECT_TITLE Honeybee metabolomics 2022 PR:PROJECT_TYPE Untargeted metabolomics PR:PROJECT_SUMMARY The impact of crop protection products on pollinating insects, particularly PR:PROJECT_SUMMARY honeybees, is a critical consideration for ecological safety. Beyond acute PR:PROJECT_SUMMARY toxicity, sublethal effects can potentially disrupt honeybee behavior, PR:PROJECT_SUMMARY compromising pollination efficiency. Traditional assessment methods, reliant on PR:PROJECT_SUMMARY visual observations, may not be optimal for capturing sublethal effects. In this PR:PROJECT_SUMMARY study, we delved into molecular mechanisms underlying sublethal effects, PR:PROJECT_SUMMARY employing three distinct platforms: whole-tissue transcriptomics, spatial PR:PROJECT_SUMMARY transcriptomics, and metabolomics. Our findings reveal valuable insights into PR:PROJECT_SUMMARY honeybee toxicity, with certain approaches demonstrating superiority in PR:PROJECT_SUMMARY elucidating tissue-specific and treatment-specific responses, especially PR:PROJECT_SUMMARY untargeted metabolomics tend to be a valuable tool for new bee-friendly PR:PROJECT_SUMMARY agrochemical development. This research will aid in screening new molecules for PR:PROJECT_SUMMARY pollinator safety and contribute to developing strategies that enhance crop PR:PROJECT_SUMMARY yield while maintaining ecological sustainability. PR:INSTITUTE Corteva Agriscience PR:LAST_NAME Yang PR:FIRST_NAME Kundi PR:ADDRESS 9330 Zionsville Rd, Indianapolis, IN 46268, Indianapolis, Indiana, 46077, USA PR:EMAIL kundi.yang@corteva.com PR:PHONE 5133063039 #STUDY ST:STUDY_TITLE Using Untargeted Metabolomics as a New Approach for Understanding Honeybee ST:STUDY_TITLE Toxicity ST:STUDY_SUMMARY The impact of crop protection products on pollinating insects, particularly ST:STUDY_SUMMARY honeybees, is a critical consideration for ecological safety. Beyond acute ST:STUDY_SUMMARY toxicity, sublethal effects can potentially disrupt honeybee behavior, ST:STUDY_SUMMARY compromising pollination efficiency. Traditional assessment methods, reliant on ST:STUDY_SUMMARY visual observations, may not be optimal for capturing sublethal effects. In this ST:STUDY_SUMMARY study, we delved into molecular mechanisms underlying sublethal effects, ST:STUDY_SUMMARY employing three distinct platforms: whole-tissue transcriptomics, spatial ST:STUDY_SUMMARY transcriptomics, and metabolomics. Our findings reveal valuable insights into ST:STUDY_SUMMARY honeybee toxicity, with certain approaches demonstrating superiority in ST:STUDY_SUMMARY elucidating tissue-specific and treatment-specific responses, especially ST:STUDY_SUMMARY untargeted metabolomics tend to be a valuable tool for new bee-friendly ST:STUDY_SUMMARY agrochemical development. This research will aid in screening new molecules for ST:STUDY_SUMMARY pollinator safety and contribute to developing strategies that enhance crop ST:STUDY_SUMMARY yield while maintaining ecological sustainability. ST:INSTITUTE Corteva Agriscience ST:LAST_NAME Yang ST:FIRST_NAME Kundi ST:ADDRESS 9330 Zionsville Rd, Indianapolis, IN 46268, Indianapolis, Indiana, 46077, USA ST:EMAIL kundi.yang@corteva.com ST:PHONE 5133063039 #SUBJECT SU:SUBJECT_TYPE Insect SU:SUBJECT_SPECIES Apis mellifera SU:TAXONOMY_ID 7460 #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data SUBJECT_SAMPLE_FACTORS Blank_1 Blank1 Sample source:SolventBlank | Sample section:Blank Genotype=Blank; RAW_FILE_NAME=Blank1.RAW SUBJECT_SAMPLE_FACTORS Blank_2 Blank2 Sample source:SolventBlank | Sample section:Blank Genotype=Blank; RAW_FILE_NAME=Blank2.RAW SUBJECT_SAMPLE_FACTORS Blank_4 Blank4 Sample source:SolventBlank | Sample section:Blank Genotype=Blank; RAW_FILE_NAME=Blank4.RAW SUBJECT_SAMPLE_FACTORS QC_1 QC1 Sample source:Honeybee | Sample section:Pooled Wild Type Genotype=Pooled Wild Type; RAW_FILE_NAME=QC1.RAW SUBJECT_SAMPLE_FACTORS QC_2 QC2 Sample source:Honeybee | Sample section:Pooled Wild Type Genotype=Pooled Wild Type; RAW_FILE_NAME=QC2.RAW SUBJECT_SAMPLE_FACTORS QC_3 QC3 Sample source:Honeybee | Sample section:Pooled Wild Type Genotype=Pooled Wild Type; RAW_FILE_NAME=QC3.RAW SUBJECT_SAMPLE_FACTORS Bee_Head_1 H-1 Sample source:Honeybee | Sample section:Wild Type_Head Genotype=Wild Type; RAW_FILE_NAME=A-1.RAW SUBJECT_SAMPLE_FACTORS Bee_Head_2 H-2 Sample source:Honeybee | Sample section:Wild Type_Head Genotype=Wild Type; RAW_FILE_NAME=A-2.RAW SUBJECT_SAMPLE_FACTORS Bee_Head_3 H-3 Sample source:Honeybee | Sample section:Wild Type_Head Genotype=Wild Type; RAW_FILE_NAME=A-3.RAW SUBJECT_SAMPLE_FACTORS Bee_Head_4 H-4 Sample source:Honeybee | Sample section:Wild Type_Head Genotype=Wild Type; RAW_FILE_NAME=A-4.RAW SUBJECT_SAMPLE_FACTORS Bee_Head_5 H-5 Sample source:Honeybee | Sample section:Wild Type_Head Genotype=Wild Type; RAW_FILE_NAME=A-5.RAW SUBJECT_SAMPLE_FACTORS Bee_Abdomen_1 A-1 Sample source:Honeybee | Sample section:Wild Type_Abdomen Genotype=Wild Type; RAW_FILE_NAME=H-1.RAW SUBJECT_SAMPLE_FACTORS Bee_Abdomen_2 A-2 Sample source:Honeybee | Sample section:Wild Type_Abdomen Genotype=Wild Type; RAW_FILE_NAME=H-2.RAW SUBJECT_SAMPLE_FACTORS Bee_Abdomen_3 A-3 Sample source:Honeybee | Sample section:Wild Type_Abdomen Genotype=Wild Type; RAW_FILE_NAME=H-3.RAW SUBJECT_SAMPLE_FACTORS Bee_Abdomen_4 A-4 Sample source:Honeybee | Sample section:Wild Type_Abdomen Genotype=Wild Type; RAW_FILE_NAME=H-4.RAW SUBJECT_SAMPLE_FACTORS Bee_Abdomen_5 A-5 Sample source:Honeybee | Sample section:Wild Type_Abdomen Genotype=Wild Type; RAW_FILE_NAME=H-5.RAW SUBJECT_SAMPLE_FACTORS Bee_Throax_1 T-1 Sample source:Honeybee | Sample section:Wild Type_Thorax Genotype=Wild Type; RAW_FILE_NAME=T-1.RAW SUBJECT_SAMPLE_FACTORS Bee_Throax_2 T-2 Sample source:Honeybee | Sample section:Wild Type_Thorax Genotype=Wild Type; RAW_FILE_NAME=T-2.RAW SUBJECT_SAMPLE_FACTORS Bee_Throax_3 T-3 Sample source:Honeybee | Sample section:Wild Type_Thorax Genotype=Wild Type; RAW_FILE_NAME=T-3.RAW SUBJECT_SAMPLE_FACTORS Bee_Throax_5 T-5 Sample source:Honeybee | Sample section:Wild Type_Thorax Genotype=Wild Type; RAW_FILE_NAME=T-5.RAW #COLLECTION CO:COLLECTION_SUMMARY Honeybee were weighted and sectioned. CO:SAMPLE_TYPE Tissues #TREATMENT TR:TREATMENT_SUMMARY Honeybee were treated with normal diet. #SAMPLEPREP SP:SAMPLEPREP_SUMMARY Honeybee sections were collected separately and then homogenised with stainless SP:SAMPLEPREP_SUMMARY steel balls. Organic/Water extraction were used for metabolite extraction. #CHROMATOGRAPHY CH:CHROMATOGRAPHY_SUMMARY Solvent A: Acetonitrile/Water 90% / 10% (v/v) + 0.2% Acetic acid (v) + 5 mM CH:CHROMATOGRAPHY_SUMMARY Ammonium acetate Solvent B: Acetonitrile/Water 10% / 90% (v/v) + 0.2% Acetic CH:CHROMATOGRAPHY_SUMMARY acid (v) + 5 mM Ammonium acetate CH:CHROMATOGRAPHY_TYPE HILIC CH:INSTRUMENT_NAME Thermo Scientific Dionex Ultimate 3000 CH:COLUMN_NAME Waters Atlantis Premier BEH Z-HILIC CH:SOLVENT_A 90% acetonitrile/10% water; 0.2% acetic acid; 5 mM ammonium acetate CH:SOLVENT_B 10% acetonitrile/90% water; 0.2% acetic acid; 5 mM ammonium acetate CH:FLOW_GRADIENT 0 - 1 min, 2% B; 6 min, 75% B; 12 min, 98% B; 12 – 15 min, 98% B CH:FLOW_RATE 0.25mL/min CH:COLUMN_TEMPERATURE 25 CH:CHROMATOGRAPHY_COMMENTS Atlantis Premier BEH Z-HILIC Cloumn, 1.7 um, 2.1 mm x 100 mm #ANALYSIS AN:ANALYSIS_TYPE MS #MS MS:INSTRUMENT_NAME Thermo Q Exactive Plus Orbitrap MS:INSTRUMENT_TYPE Orbitrap MS:MS_TYPE ESI MS:ION_MODE NEGATIVE MS:MS_COMMENTS - MS:MS_RESULTS_FILE ST003212_AN005268_Results.txt UNITS:counts Has m/z:Yes Has RT:No RT units:No RT data #END