#METABOLOMICS WORKBENCH spinelli_lab_20240514_125536 DATATRACK_ID:4843 STUDY_ID:ST003214 ANALYSIS_ID:AN005271 PROJECT_ID:PR001988 VERSION 1 CREATED_ON May 21, 2024, 11:49 am #PROJECT PR:PROJECT_TITLE Rhodoquinone is an Electron Carrier in the Mammalian Electron Transport Chain PR:PROJECT_SUMMARY Ubiquinone (UQ), the only known electron carrier in the mammalian electron PR:PROJECT_SUMMARY transport chain (ETC), delivers electrons to both oxygen (O2) and fumarate as PR:PROJECT_SUMMARY terminal electron acceptors. As fumarate has a lower reduction potential than PR:PROJECT_SUMMARY UQ, fumarate reduction is only thermodynamically favorable when ubiquinol, the PR:PROJECT_SUMMARY reduced form of UQ, accumulates. Paradoxically, some tissues reduce fumarate PR:PROJECT_SUMMARY without ubiquinol buildup, suggesting another mechanism enables fumarate PR:PROJECT_SUMMARY reduction in mammals. Here, we identify rhodoquinone (RQ), a novel component of PR:PROJECT_SUMMARY the mammalian ETC that carries electrons to fumarate, instead of O2, as the PR:PROJECT_SUMMARY terminal electron acceptor. RQ, which is undetectable in cultured mammalian PR:PROJECT_SUMMARY cells, is enriched in mitochondria from mouse and human tissues that catalyze PR:PROJECT_SUMMARY high levels of fumarate reduction. UQ and RQ-directed ETC circuits support PR:PROJECT_SUMMARY unique programs of mitochondrial function. Through expression of a bacterial PR:PROJECT_SUMMARY enzyme that converts UQ into RQ and development of a novel RQ analog, we PR:PROJECT_SUMMARY demonstrate that reprogramming the mammalian ETC from the UQ to RQ circuit PR:PROJECT_SUMMARY renders cells highly resistant to hypoxia exposure in vitro and in vivo. Thus, PR:PROJECT_SUMMARY we establish RQ as a fundamental component of the mammalian ETC and unveil that PR:PROJECT_SUMMARY reprogramming the ETC to the RQ-circuit is a tractable strategy to ameliorate PR:PROJECT_SUMMARY hypoxia-related conditions. PR:INSTITUTE UMass Chan Medical School PR:LAST_NAME UMass Chan PR:FIRST_NAME Spinelli Lab PR:ADDRESS 55 Lake Avenue North, Worcester, Massachusetts, 01605, USA PR:EMAIL spinellilab@gmail.com PR:PHONE (508) 856-8989 ext. 68148 #STUDY ST:STUDY_TITLE Glutamine Tracing Assay in RquA AAV Injected Mice with a Standard Curve ST:STUDY_SUMMARY The extraction of RquA AAV injected livers from C57BL/6 mice. Metabolites from ST:STUDY_SUMMARY the liver were used to measure fumarate reduction and succinate oxidation. ST:STUDY_SUMMARY Glutamine tracing was used to determine forward and reverse direction of the TCA ST:STUDY_SUMMARY cycle. Standards were used to create a standard curve by diluting down a stock ST:STUDY_SUMMARY of fumarate and succinate standard mixture. The standard curve was used to ST:STUDY_SUMMARY determine picomoles of metabolites in the TCA cycle. ST:INSTITUTE UMass Chan Medical School ST:LAST_NAME UMass Chan ST:FIRST_NAME Spinelli Lab ST:ADDRESS 55 Lake Avenue North, Worcester, Massachusetts, 01605, USA ST:EMAIL spinellilab@gmail.com ST:PHONE (508) 856-8989 ext. 68148 #SUBJECT SU:SUBJECT_TYPE Mammal SU:SUBJECT_SPECIES Mus musculus SU:TAXONOMY_ID 10090 #FACTORS #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data SUBJECT_SAMPLE_FACTORS - Standard 2 Sample source:Succinate and Fumarate Reagent Mixture | Standards for Standard Curve and Treatment:Measurable Standard Curve RAW_FILE_NAME(File Name )=RquA_AAV_mouse_polar01 SUBJECT_SAMPLE_FACTORS - Standard 3 Sample source:Succinate and Fumarate Reagent Mixture | Standards for Standard Curve and Treatment:Measurable Standard Curve RAW_FILE_NAME(File Name )=RquA_AAV_mouse_polar02 SUBJECT_SAMPLE_FACTORS - Standard 4 Sample source:Succinate and Fumarate Reagent Mixture | Standards for Standard Curve and Treatment:Measurable Standard Curve RAW_FILE_NAME(File Name )=RquA_AAV_mouse_polar03 SUBJECT_SAMPLE_FACTORS - Standard 5 Sample source:Succinate and Fumarate Reagent Mixture | Standards for Standard Curve and Treatment:Measurable Standard Curve RAW_FILE_NAME(File Name )=RquA_AAV_mouse_polar04 SUBJECT_SAMPLE_FACTORS - Standard 6 Sample source:Succinate and Fumarate Reagent Mixture | Standards for Standard Curve and Treatment:Measurable Standard Curve RAW_FILE_NAME(File Name )=RquA_AAV_mouse_polar05 SUBJECT_SAMPLE_FACTORS - Standard 7 Sample source:Succinate and Fumarate Reagent Mixture | Standards for Standard Curve and Treatment:Measurable Standard Curve RAW_FILE_NAME(File Name )=RquA_AAV_mouse_polar06 SUBJECT_SAMPLE_FACTORS - Standard 8 Sample source:Succinate and Fumarate Reagent Mixture | Standards for Standard Curve and Treatment:Measurable Standard Curve RAW_FILE_NAME(File Name )=RquA_AAV_mouse_polar07 SUBJECT_SAMPLE_FACTORS - Liver 1 Sample source:C57/BL6 mouse liver tissue | Standards for Standard Curve and Treatment:RquA AAV injected RAW_FILE_NAME(File Name )=RquA_AAV_mouse_polar08 SUBJECT_SAMPLE_FACTORS - Liver 2 Sample source:C57/BL6 mouse liver tissue | Standards for Standard Curve and Treatment:RquA AAV injected RAW_FILE_NAME(File Name )=RquA_AAV_mouse_polar09 SUBJECT_SAMPLE_FACTORS - Liver 3 Sample source:C57/BL6 mouse liver tissue | Standards for Standard Curve and Treatment:RquA AAV injected RAW_FILE_NAME(File Name )=RquA_AAV_mouse_polar10 SUBJECT_SAMPLE_FACTORS - Liver 4 Sample source:C57/BL6 mouse liver tissue | Standards for Standard Curve and Treatment:RquA AAV injected RAW_FILE_NAME(File Name )=RquA_AAV_mouse_polar11 SUBJECT_SAMPLE_FACTORS - Liver 5 Sample source:C57/BL6 mouse liver tissue | Standards for Standard Curve and Treatment:RquA AAV injected RAW_FILE_NAME(File Name )=RquA_AAV_mouse_polar12 SUBJECT_SAMPLE_FACTORS - Liver 6 Sample source:C57/BL6 mouse liver tissue | Standards for Standard Curve and Treatment:RquA AAV injected RAW_FILE_NAME(File Name )=RquA_AAV_mouse_polar13 SUBJECT_SAMPLE_FACTORS - Liver 7 Sample source:C57/BL6 mouse liver tissue | Standards for Standard Curve and Treatment:Vehicle RAW_FILE_NAME(File Name )=RquA_AAV_mouse_polar14 SUBJECT_SAMPLE_FACTORS - Liver 8 Sample source:C57/BL6 mouse liver tissue | Standards for Standard Curve and Treatment:Vehicle RAW_FILE_NAME(File Name )=RquA_AAV_mouse_polar15 SUBJECT_SAMPLE_FACTORS - Liver 9 Sample source:C57/BL6 mouse liver tissue | Standards for Standard Curve and Treatment:Vehicle RAW_FILE_NAME(File Name )=RquA_AAV_mouse_polar16 SUBJECT_SAMPLE_FACTORS - Liver 10 Sample source:C57/BL6 mouse liver tissue | Standards for Standard Curve and Treatment:Vehicle RAW_FILE_NAME(File Name )=RquA_AAV_mouse_polar17 SUBJECT_SAMPLE_FACTORS - Liver 11 Sample source:C57/BL6 mouse liver tissue | Standards for Standard Curve and Treatment:Vehicle RAW_FILE_NAME(File Name )=RquA_AAV_mouse_polar18 SUBJECT_SAMPLE_FACTORS - Liver 12 Sample source:C57/BL6 mouse liver tissue | Standards for Standard Curve and Treatment:Vehicle RAW_FILE_NAME(File Name )=RquA_AAV_mouse_polar19 SUBJECT_SAMPLE_FACTORS - Liver 13 Sample source:C57/BL6 mouse liver tissue | Standards for Standard Curve and Treatment:Vehicle RAW_FILE_NAME(File Name )=RquA_AAV_mouse_polar20 SUBJECT_SAMPLE_FACTORS - Liver 15 Sample source:C57/BL6 mouse liver tissue | Standards for Standard Curve and Treatment:Vehicle RAW_FILE_NAME(File Name )=RquA_AAV_mouse_polar21 #COLLECTION CO:COLLECTION_SUMMARY 13C515N2-glutamine (Cambridge Isotope labs) was resuspended to 50 mg/mL in 1X CO:COLLECTION_SUMMARY PBS. C57BL/6 mice between the ages of 10-16 weeks were weighed to allow CO:COLLECTION_SUMMARY injection of 0.75 g/kg intraperitoneally and 0.2 g/kg intravenously via the tail CO:COLLECTION_SUMMARY vein. Mice were injected with the respective amounts of 13C515N2-glutamine, were CO:COLLECTION_SUMMARY sacrificed after 45 minutes, and tissues were harvested and flash frozen in CO:COLLECTION_SUMMARY Liquid Nitrogen. Samples were stored at -80°C until their extraction and run on CO:COLLECTION_SUMMARY the polar LC-MS method. CO:SAMPLE_TYPE Mouse Liver #TREATMENT TR:TREATMENT_SUMMARY Adeno-associated viruses (AAVs) were used for expression of RquA in mice. TR:TREATMENT_SUMMARY Briefly, rAAV was produced by transient HEK 293 cell transfection and CsCl TR:TREATMENT_SUMMARY sedimentation by the University of Massachusetts Medical School Viral Vector TR:TREATMENT_SUMMARY Core. Vector genome titers of the rAAV preparations for control virus TR:TREATMENT_SUMMARY (AAV9.Tre3G-mCherry SV40 CB6 Tet-on 3G RBG) and RquA virus (AAV9.Tre3G-RquA SV40 TR:TREATMENT_SUMMARY CB6 Tet-on 3G RBG) were determined by ddPCR, and purity was assessed by 4%–12% TR:TREATMENT_SUMMARY SDS-acrylamide gel electrophoresis and silver staining (Invitrogen). Mice were TR:TREATMENT_SUMMARY intravenously injected with 6 X 10^11 genome copies of either virus and given 1 TR:TREATMENT_SUMMARY mg/mL doxycycline in the drinking water for 7 days to induce either mCherry or TR:TREATMENT_SUMMARY RquA expression. #SAMPLEPREP SP:SAMPLEPREP_SUMMARY Tissues were flash frozen in liquid nitrogen and powderized using a mortar and SP:SAMPLEPREP_SUMMARY pestle. Approximately 10 mg of tissue powder was transferred into an Eppendorf SP:SAMPLEPREP_SUMMARY tube. The tissue powder was re-suspended in 800 µL pre-cooled HPLC-grade 60:40 SP:SAMPLEPREP_SUMMARY Methanol:Water (Sigma) and then vortexed for 15 minutes at 4°C. 500 µL of SP:SAMPLEPREP_SUMMARY pre-cooled LC-MS grade chloroform (Sigma) was added to the lysate and again SP:SAMPLEPREP_SUMMARY vortexed for 15 minutes at 4°C. Samples were then centrifuged at 16,000 x g for SP:SAMPLEPREP_SUMMARY 10 minutes at 4°C, creating three layers: the top layer containing polar SP:SAMPLEPREP_SUMMARY metabolites, the middle layer containing protein, and the bottom layer SP:SAMPLEPREP_SUMMARY containing non-polar metabolites. The top layer was transferred into a new SP:SAMPLEPREP_SUMMARY Eppendorf tube, dried down in a Refrigerated CentriVap Benchtop Vacuum SP:SAMPLEPREP_SUMMARY Concentrator connected to a CentriVap-105 Cold Trap (Labconco), and stored at SP:SAMPLEPREP_SUMMARY -80°C until they were re-suspended for LC-MS analysis. The non-polar layer was SP:SAMPLEPREP_SUMMARY discarded, and the remaining protein layer was saved for protein quantification. SP:SAMPLEPREP_SUMMARY The protein layer from the metabolite isolation was re-suspended in 1 mL of RIPA SP:SAMPLEPREP_SUMMARY buffer (150 mM NaCl, 50 mM Tris HCl pH 7.5, 0.1% SDS, 1% Triton-X 100 (Sigma), SP:SAMPLEPREP_SUMMARY 0.5% deoxycholate (Sigma), cOmplete EDTA-free protease inhibitor (Sigma)). The SP:SAMPLEPREP_SUMMARY samples were vortexed for 10 minutes at 4°C and then centrifuged at 16,000 x g SP:SAMPLEPREP_SUMMARY for 10 minutes at 4°C. Protein concentrations for each sample were calculated SP:SAMPLEPREP_SUMMARY using the Pierce BCA Protein Assay Kit (Life Technologies). The protein SP:SAMPLEPREP_SUMMARY concentrations were used to calculate the re-suspension volume to normalize all SP:SAMPLEPREP_SUMMARY samples for LC-MS analysis to equal (1 µg/µL ) concentration. #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE HILIC CH:INSTRUMENT_NAME Thermo Vanquish CH:COLUMN_NAME Merck SeQuant ZIC-HILIC (150 x 2.1mm,5um) CH:SOLVENT_A 100% water; 0.1% ammonium hydroxide; 20mM ammonium carbonate CH:SOLVENT_B 100% acetonitrile CH:FLOW_GRADIENT 20 min, 80% - 20% B; 0.5 min, 20% - 80% B; 7.5min, 80% B CH:FLOW_RATE 0.15ml/min CH:COLUMN_TEMPERATURE 25 #ANALYSIS AN:ANALYSIS_TYPE MS #MS MS:INSTRUMENT_NAME Thermo Q Exactive Plus Orbitrap MS:INSTRUMENT_TYPE Orbitrap MS:MS_TYPE ESI MS:ION_MODE NEGATIVE MS:MS_COMMENTS The mass spectrometer had the spray voltage set to 4.0 kV, heated capillary to MS:MS_COMMENTS 350°C, and the HESI probe at 30 °C. The sheath gas flow was set at 10 units, MS:MS_COMMENTS auxiliary gas at 1 units, and sweep gas flow at 1 unit. An additional scan MS:MS_COMMENTS between 220-700 m/z was used to enhance nucleotide detection in the negative MS:MS_COMMENTS mode as well with the maximum injection time set to 80 msec. Data acquired by MS:MS_COMMENTS Thermo Fisher's Xcalibur software and analyzed by their Tracefinder software. MS:MS_COMMENTS The raw files provided contain data from both positive and negative ion mode. #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS peak area MS_METABOLITE_DATA_START Samples Standard 2 Standard 3 Standard 4 Standard 5 Standard 6 Standard 7 Standard 8 Liver 1 Liver 2 Liver 3 Liver 4 Liver 5 Liver 6 Liver 7 Liver 8 Liver 9 Liver 10 Liver 11 Liver 12 Liver 13 Liver 15 Factors Sample source:Succinate and Fumarate Reagent Mixture | Standards for Standard Curve and Treatment:Measurable Standard Curve Sample source:Succinate and Fumarate Reagent Mixture | Standards for Standard Curve and Treatment:Measurable Standard Curve Sample source:Succinate and Fumarate Reagent Mixture | Standards for Standard Curve and Treatment:Measurable Standard Curve Sample source:Succinate and Fumarate Reagent Mixture | Standards for Standard Curve and Treatment:Measurable Standard Curve Sample source:Succinate and Fumarate Reagent Mixture | Standards for Standard Curve and Treatment:Measurable Standard Curve Sample source:Succinate and Fumarate Reagent Mixture | Standards for Standard Curve and Treatment:Measurable Standard Curve Sample source:Succinate and Fumarate Reagent Mixture | Standards for Standard Curve and Treatment:Measurable Standard Curve Sample source:C57/BL6 mouse liver tissue | Standards for Standard Curve and Treatment:RquA AAV injected Sample source:C57/BL6 mouse liver tissue | Standards for Standard Curve and Treatment:RquA AAV injected Sample source:C57/BL6 mouse liver tissue | Standards for Standard Curve and Treatment:RquA AAV injected Sample source:C57/BL6 mouse liver tissue | Standards for Standard Curve and Treatment:RquA AAV injected Sample source:C57/BL6 mouse liver tissue | Standards for Standard Curve and Treatment:RquA AAV injected Sample source:C57/BL6 mouse liver tissue | Standards for Standard Curve and Treatment:RquA AAV injected Sample source:C57/BL6 mouse liver tissue | Standards for Standard Curve and Treatment:Vehicle Sample source:C57/BL6 mouse liver tissue | Standards for Standard Curve and Treatment:Vehicle Sample source:C57/BL6 mouse liver tissue | Standards for Standard Curve and Treatment:Vehicle Sample source:C57/BL6 mouse liver tissue | Standards for Standard Curve and Treatment:Vehicle Sample source:C57/BL6 mouse liver tissue | Standards for Standard Curve and Treatment:Vehicle Sample source:C57/BL6 mouse liver tissue | Standards for Standard Curve and Treatment:Vehicle Sample source:C57/BL6 mouse liver tissue | Standards for Standard Curve and Treatment:Vehicle Sample source:C57/BL6 mouse liver tissue | Standards for Standard Curve and Treatment:Vehicle 10-Hydroxydecanoic acid 0 69509.60536 89614.43598 42915.25712 45544.33924 59169.70521 71981.31488 320104.0017 194374.5558 243696.7652 514976.9941 1124833.938 84569.23368 227238.2956 1598568.259 447639.7287 467347.0331 701454.2157 408195.7787 861003.4729 353607.1717 2-Deoxy-glucose 90597.89363 407119.1837 155164.784 86398.01204 55775.27298 54678.40454 275495.6372 3594079.251 2799244.977 522179.9796 1226485.714 0 3012330.518 0 0 0 0 0 0 0 0 2-Hydroxybutyric acid 73774.93392 152258.8475 54970.67649 98907.42278 118278.2268 118150.0169 76907.68409 62211926.67 53113160.42 17305883.48 70613956.17 119812290.8 69921911.67 200824574.9 166193111.2 27881963.84 48338988.05 77347840.13 34402064.5 62149272.09 44167218.46 2-Methylglutaric acid 104869.5332 668492.6833 344935.5511 152982.0952 335584.981 50485.25726 548286.152 6107688.253 3501482.702 2156931.099 1876925.586 7572227.964 2918837.84 4021647.198 2560082.999 981569.8417 4186433.536 1234519.155 819642.5038 1002069.465 887575.9417 3-(2-Hydroxyphenyl)propanoic acid 29022.9975 70192.04739 63852.75569 46601.74224 28381.45877 17121.67022 7008.187724 737289.3653 1024223.297 4960933.167 643041.0798 544837.5987 898151.159 2832656.286 1808407.063 457917.7047 623561.4821 753242.4178 540714.7487 666558.4905 415225.2811 Tetrahydrocortisol 4716.715316 0 0 0 0 0 1123.001619 4105824.097 3996202.173 1592944.518 2278764.837 7039927.915 1829195.201 9019223.154 9159748.487 1265284.141 2864091.15 4050087.59 2323693.277 3504205.58 2238225.72 3-Hydroxybenzaldehyde 1925551.097 1906370.074 0 1475381.236 1557855.549 0 0 145381428.9 140733644.5 112285017.6 48185140.85 28538471.94 50736252.21 73016847.85 14948214.34 9537833.52 32285049.41 27585035.75 18493104.91 9642025.811 16659527.26 3-Hydroxybenzoic acid 29466.62907 84270.25873 34087.15005 32208.90887 63539.23328 57497.90361 51583.23703 2730759.142 2274910.517 2634616.636 3778001.088 640589.5481 2136378.952 2978577.269 1514127.615 273377.6235 783989.4015 701844.7379 523774.4556 658465.8612 440821.7907 3E-Methylglutaconic acid 30044.4336 31143.46396 0 0 53041.41782 86697.65656 34075.8025 11295439.36 6734158.969 3921349.715 10348253.7 19763216.53 11259694.68 74935014.46 67027881.25 19007312.06 19659634.19 30100813.83 25605169.61 31185296.21 23798483.49 3-Phosphoglyceric acid 4000.106959 0 0 0 0 0 0 5742353.572 7038697.045 14702.85986 182243.7136 33334021.93 328684.1132 2438553.467 1665282.966 1365247.3 1949040.258 8137350.683 9155174.917 2431144.206 2648240.909 3-Sulfino-alanine 61757.51103 51716.11817 51752.90311 57633.14649 47184.01433 20010.42519 31079.59824 6502784.37 5187873.132 3348451.703 2648579.976 2106870.629 4716992.152 1942722.827 2346991.03 1527082.345 1514569.29 1600794.851 1129626.999 1939795.222 1904683.741 P-Aminobenzoic acid 25374.81887 22660.04016 47889.86756 65893.88691 17588.29795 55196.45545 52705.64837 1131990.927 685007.3228 619116.5587 2512944.112 3483749.536 930107.263 18162050.14 22412859.71 7181125.29 2843953.59 14594863.56 7016355.77 11033106.31 2622475.794 Vanylglycol 16366035.69 8596955.521 7478663.477 6330709.114 9295184.438 5806962.59 7561085.386 13133780.87 17569669.04 8794690.544 10136052.67 17152419.56 15940072.08 12959379.52 12174107.86 14111482.48 11814751.29 14289692 15843722.6 13092490.65 9622368.611 4-Hydroxybenzaldehyde 1155168.118 477840.8372 489027.1811 50237.67954 679375.5343 200345.49 763262.3525 7716734.266 9496724.14 18392579.42 6407723.796 12120820.73 6279104.885 8757652.41 8417910.808 4420812.516 5140868.128 4525856.842 3203778.31 3418593.395 3576772.201 4-Hydroxybenzoic acid 27867.86335 78961.28419 45457.61542 28381.03674 41436.6875 48494.33101 31456.12802 2644345.523 2244115.949 2422338.474 3589546.024 640376.0788 2187839.52 2978577.269 1514127.615 254915.1832 740951.3456 681434.5028 463330.7801 658000.8488 320546.2122 4-Pyridoxic acid 23721.84368 60469.08603 38151.23477 41352.05964 98408.98955 51380.35316 43098.43395 1184539.081 1288592.451 994282.8346 1283515.65 1210222.013 1131566.557 4116329.847 6766583.552 1031403.8 1728215.455 2501962.466 1126880.151 1553958.501 1145164.354 5-Hydroxyindoleacetic acid 0 0 0 0 0 0 0 264802.2489 158894.075 27523.69017 133270.3618 319304.2497 110118.2264 1674033.232 1101546.269 237581.6074 349585.9715 427337.1776 351895.1257 460448.2514 210262.6943 6-Hydroxynicotinic acid 68444.30776 39828.61298 32429.06844 29343.26005 60871.41965 33629.57607 16645.98821 211534.7271 111705.3786 114190.9745 469972.6834 1340839.947 289476.9388 5900015.066 3032810.307 807847.9512 1099348.097 1418657.983 1165930.718 1546926.204 797905.2703 6-Phosphogluconic acid 2253.030174 1219.582399 790.9575211 765.1903028 0 0 867.3875283 2846989.37 1617124.365 6099.357912 507811.9499 9164769.345 780356.0649 9003869.499 5349448.731 1855278.672 2820543.944 5860139.126 4452387.776 4412976.716 2690693.724 Adenosine 22933.73634 26217.18843 16861.50587 43148.65887 467779.4336 502292.8588 9546.425593 21242673.52 21867175.92 129881588.4 55716722.92 81672869.21 43547662.27 199061435.9 185590467.4 61410587.46 35016352.93 52575572.77 59538970.39 59316835.19 16897726.61 2',3' cyclic AMP 826.1255302 0 0 0 0 0 0 5516416.145 4066751.725 2777683.238 2982060.462 3212637.484 5980981.811 5203806.059 4755326.471 1359264.158 1328561.979 2129171.294 1273106.113 1954794.809 1006077.05 Adenosine 3',5'-diphosphate 421753952.8 205307361.2 81504119.53 28783120.66 2655217.194 835763.7269 642412.2352 102921720.6 47977152.61 152161.6088 330018.6421 496305657.2 3956459.67 143595291.2 140858015.5 157147937.9 182941104.4 477190486.3 352905944.2 301415450.2 220512674.8 ADP-ribose 29556.17744 7287.725325 0 1051.366316 0 0 0 544911.6228 2629432.533 87467.55086 220851.7071 38508998.06 350106.9939 324873.1873 423515.8445 3666318.554 180385.7906 585680.9921 13418118.02 2378330.677 769037.4212 ATP 197149747.7 81626028.25 29480522.64 8966937.626 743065.7283 0 99229.86063 5784105.317 5885706.965 41033.50909 5204.532851 271002564.2 598294.121 5444206.694 12014228.6 42918173.72 36933924.02 238312674.7 231540961.9 110841121.5 113817185.6 AMP 11352435.79 5830411.819 2666789.099 1198843.182 2337519.954 1388713.149 79267.68855 1780903582 1183789557 19212454.08 1129118446 1726367448 1375513458 4277358390 3854800836 1270557169 1332279604 1891484112 1066274483 1733240484 1201235207 Adipic acid 102158.2171 636670.1636 344935.5511 152982.0952 345108.7606 77446.83452 548286.152 6104962.887 3501482.702 2156931.099 1876925.586 7572227.964 2918837.84 4021647.198 2560082.999 981569.8417 4194026.88 1234519.155 805220.9735 1001591.064 887575.9417 Allantoin 31788.32039 0 0 0 0 0 0 23855848.18 21214732.57 30514635.67 25879869.8 27313573.85 27932540.1 66737903.15 43935867.93 13146119.49 18805326.93 15716183.64 16711148.01 22871923.73 13767859.82 Allose 274448.3986 46785.2816 43459.31799 83382.38899 76914.7612 86435.38066 156733.0945 61269207.23 34480606.86 19835150.16 57673114.04 114805997.9 61107535.04 580858326.2 443724078.9 111834509.2 119963596.8 186114206.4 147873186.5 194071263.2 137864119.3 Glucose 208128.9181 224933.9533 0 111306.296 0 89210.33583 155836.7321 63908245.54 37563947.91 22077244.57 58773228.33 123331807.4 61895489.43 642307974.5 505626229.4 115343203.8 122774618.5 214097192.6 169763247.7 230052658.4 159926099.9 Arabitol 184741.6193 84305.62807 83701.94175 20167.61067 0 53495.65222 63149.89218 2531852.8 2245787.551 2966377.493 3803100.986 2505668.307 3522033.915 6279026.717 6492066.192 2948598.383 2979864.315 2627882.208 2146111.077 2783100.515 2122973.654 Vitamin C 52341.37234 68461.36172 61042.79533 29152.87264 227944.8398 110379.9087 49771.58794 590420.023 746181.8252 388973.7731 778408.7413 476439.1202 672369.2422 2228343.936 2918537.599 717825.9859 359840.3617 1145044.503 638508.5122 1202173.857 466104.4972 Aspartic acid 141809659.1 73848929.08 27065441.43 8769386.813 1010860.267 488494.7622 334763.9315 335392152.3 320707175 244183931.8 286274770.9 108555981 392040860.6 320020325.5 244980687.9 65376273.96 81210513.16 109350314.9 77147482.29 95625812.41 70301193.15 Aspartic acid_13C1 6239176.178 3117000.961 1126444.968 341449.8265 13224.99408 0 8938.475029 48065266.88 41501304.31 25742492.4 40467914.39 10997581.7 52647396.41 31766620.61 12969801.75 5392897.901 8795347.956 9333343.082 7763809.828 6550592.417 6426690.229 Aspartic acid_13C2 1058263.202 478723.3271 199133.1623 110322.7992 42851.21177 183044.1845 306051.0566 37712495.34 35268680.77 21476342.49 22237841.75 4694256.406 32677004.4 12120653.4 3823253.87 2551312.306 4123252.837 4891399.951 3834270.691 2296537.056 3322762.777 Aspartic acid_13C3 90762.67403 107483.1882 73084.95829 31204.21261 57946.43276 69090.27146 44414.57363 33159450.08 25483564.87 23014780.88 12964205.19 2695312.337 19502412.96 7471702.187 1277270.93 1113375.008 2481110.557 1941442.892 1937387.61 863691.0333 1880228.114 Aspartic acid_13C4 33354.95655 65949.83053 85139.01879 44032.92991 62050.11309 46400.01615 55217.23427 42276763.4 45837041.22 39792648.39 20974946.81 2903587.274 33879581.51 9783607.997 1243514.351 1473381.417 3745694.132 3964504.981 2468890.379 1215741.203 2228751.032 Aspartic acid_15N1 523220.37 239851.1507 76670.25083 0 0 0 0 98963031.24 98657859.46 78495036.61 57314333.73 13281686.56 96097680.01 40391947.38 7681866.045 4646614.28 14043288.6 12238474.03 8939008.569 4756544.183 7679411.338 Azelaic acid 485712.0056 512655.8689 445640.4583 241120.497 88380.92799 90223.96225 188563.6138 9772888.695 7340599.737 3890275.547 7399676.495 8750718.333 4423567.789 14419451.36 6663026.807 2348052.071 4033177.04 3493054.863 2501076.805 3162335.265 2295434.012 sn-Glycero-2-phosphate 114211.0951 7074.967178 4467.627979 19742.00616 5385.605893 9125.877696 0 141592469.1 118503427.6 14145234.35 307364795.7 430500594.6 196191615.8 1422339522 1075626072 326929521 327035755.3 430002991.7 347857718.1 554960514.4 283237755.1 Butyric acid 134828.3877 78316.85453 0 0 60604.58075 0 0 24571432.3 17847420.96 12759854.09 12883047.72 1997793.932 11655479.5 2680625.852 2126998.274 545691.0077 425941.0902 758940.6408 658434.1288 915828.3346 226211.2794 CDP-Ethanolamine 0 0 0 0 0 0 0 23454549.88 18741246.43 14187234.72 18608184.03 27703811.85 23965195.44 80594348.04 89241479.05 19999618.56 18370540.64 38582399.71 21958475.52 33526251.4 16509763.34 Cellobiose 33859713.53 206547.43 768382.1538 50416.33257 57439.24718 111345.332 40230.72073 2423343.794 2225181.287 1004065.35 3007777.086 33001512.18 3659089.619 510661493.6 75416675.57 16707147.36 11966469.67 157746328.9 13588692.45 38414973.87 34694946.34 Citramalic acid 728610.9395 441720.9413 400296.9244 0 8177.571348 0 0 180126664.9 163154641.4 138163872.4 113512883.1 85489447.26 137885622.4 177918747.7 136923054.7 96174199.42 83221900.52 106442866.8 92714052.7 120456551.7 80995924.28 Citric acid 914212406.6 346755518.4 99458965.65 26999725.52 4170214.049 1494602.242 627200.176 307284880.3 254133259.9 360283008.6 229522442.3 338800130.2 385376677.5 531947576.7 569142093.9 144451660.4 162536122.7 293310689.3 169585670 187328912.6 147278514.4 Citric acid_13C1 60071029.82 23731706.09 6371795.218 1637432.773 153773.9442 39630.95511 4950.988614 56779495.99 46182727.77 49123050.44 45183468.05 64689452.08 80578780.54 88442750.83 55763113.52 21265927.74 30648853.54 58688677.37 34000192.84 24209073.19 26286847.28 Citric acid_13C2 12491477.45 4847432.03 1215186.082 274670.7984 0 4304.546177 0 27263878.98 23826115.37 22103922.32 17138380.9 19000405.06 31564807.66 28415825.41 10529533.86 5975964.043 10617132.63 20995869.35 11853913.48 5511623.128 8408511.755 Citric acid_13C3 772772.4182 247122.9995 32186.19149 0 0 0 0 24954512.16 19380786.79 23682977.49 11557511.15 14903985.7 21804523.24 16502092.68 3963190.163 2979783.91 6519854.33 12792172.11 7034279.104 3431813.434 5112499.087 Citric acid_13C4 0 0 0 4638.524646 0 0 0 29533017.64 27403816.07 31659025.91 13918529.4 11461184.88 26555682.37 19987779.68 6149659.422 3945864.576 8368728.112 15425986.45 7085532.896 5303940.395 6578678.749 Citric acid_13C5 0 0 0 0 0 0 0 43328053.4 20582313.37 25383819.31 14008548.34 19570573.47 25762396.53 12155472.61 4903497.193 3470918.821 7149187.841 16197671.14 8797990.862 2770167.431 5712388.075 Citric acid_13C6 0 0 0 0 0 0 0 7564380.842 3831978.963 3296047.784 2134967.412 2472786.955 4970133.866 1556124.774 62915.24593 530044.2675 1160460.37 2211978.651 1570937.588 215108.8709 703376.9705 Cortisol 0 0 0 0 0 0 0 64808.48531 201008.2318 1671930.116 2072514.843 44284000.97 21379.32472 3957443.026 17166050.29 1922409.694 3259829.192 5626214.12 2873878.216 4564086.501 3930617.224 3',5' cyclic AMP 826.1255302 0 0 0 0 0 0 5514188.459 4066751.725 2777683.238 2982060.462 3212637.484 5980981.811 5203806.059 4755326.471 1359264.158 1328561.979 2129171.294 1273106.113 1954794.809 1006077.05 CDP 0 0 0 0 0 0 0 1362458.569 414259.6919 0 0 4713436.986 37426.83202 1128827.076 1672794.579 1355256.023 2582663.489 5744784.298 3141556.836 2373424.963 1600843.947 CMP 9884.451519 826.1619123 0 0 0 0 0 29489521.29 20376933.61 4979031.966 21395384.27 28007719.9 30150672.63 87242740.18 109643249.1 23899700.39 25165012.79 53623422.71 24350143.19 34986281.18 18962464.75 CTP 0 0 0 0 0 0 0 27847.80627 2428.231713 0 0 1647430.036 0 23383.32064 69757.64914 293298.8374 328294.2864 2461052.37 1658575.53 673103.3249 474446.8874 Deoxycytidine 46666.05835 79842.3899 33790.79478 32298.32071 45736.37089 46416.12797 37878.53939 2791279.091 1695651.525 2891373.548 1804757.584 1502678.486 2144532.634 2547375.913 1637684.953 560936.4182 691588.1409 541361.84 435618.7967 671833.9118 423281.847 dCMP 0 0 0 0 0 0 0 2489646.109 1905826.616 194262.8505 2030534.315 1533184.434 2227591.679 4767661.299 4573372.922 966051.6666 1085939.224 1726504.284 986997.1008 1439538.34 809901.0703 Deoxyuridine 85951.56307 135196.3867 124239.3256 175241.0644 59557.061 66702.13016 188035.3677 9615943.75 7359705.066 5466333.844 1969327.187 3066420.772 3948655.659 9577829.978 8145243.96 1765551.949 2373899.534 3829691.361 2184436.886 2483653.902 1174648.103 Glucuronolactone 52341.37234 68461.36172 88046.13984 29152.87264 0 110379.9087 49771.58794 586534.7579 746181.8252 388973.7731 778408.7413 476439.1202 672369.2422 2228343.936 2918537.599 717825.9859 359840.3617 1165700.849 638508.5122 1202173.857 466104.4972 Diacetyl 663050.3447 462674.5048 96526.76102 74414.54488 0 151747.8778 77814.182 28421723.86 37702243.4 10163023.72 17225538.25 7496684.923 17457268.16 5681658.462 4558277.903 3656153.969 3382446.247 4266748.086 3129334.275 4848807.765 3323753.362 4,5-Dihydroorotic acid 60184.64406 5355.644491 12376.20229 5461.81365 4575.678097 5126.697626 3909.188085 3336893.989 1780155.949 1299545.381 3294699.599 1219779.52 3339615.153 9778791.527 8987710.476 460361.6238 256475.9097 2245414.544 1271417.872 1728200.711 281700.1956 Dihydroxyacetone phosphate 41863634.32 22116451.95 8428887.472 2355746.198 0 0 58115.75501 3986932.848 2922554.814 163604.5512 1688765.484 15065880.78 5897852.905 2040016.353 12699074.13 4757552.134 3054336.853 8751884.195 5799216.285 8970513.703 3445084.654 Ribose 5-phosphate 6613.400446 1357.599204 0 5394.141417 0 0 0 81127565.47 52415489.13 9723843.488 37973352.39 130312838.1 87401623.87 202253663 168408507.7 43172851.03 34641038.85 82957479.48 46149047.32 65397355.28 41139016.47 beta-D-sedoheptulose 0 3899.259485 0 15822.4686 7563.6625 0 0 489517.7776 423603.0752 897168.5881 1592927.985 1110239.427 926593.9292 3938097.404 3943499.053 1165002.203 1811663.115 1432941.337 1439127.268 2311218.501 1778798.291 Ethylmalonic acid 123408.1952 426062.5066 67217.38604 58847.70712 50806.66253 50815.34458 156771.8487 300865342.6 218882798.3 147416946.7 154857366 25293071.38 143418980.5 23105712.21 25747940.68 4732335.607 4045111.753 7676079.846 6263348.149 8167365.753 3731379.401 Fructose 6-phosphate_13C1 0 0 0 0 0 0 0 5570286.767 2279827.991 985815.015 4313571.855 15284735.68 4549726.451 27698460.89 22254666.91 5036201.182 6288574.433 11530852 7353417.535 10921906.31 6754473.796 Fructose 6-phosphate_13C2 0 0 0 0 0 0 0 3417125.476 807763.0584 310532.0642 1043157.592 5806939.151 1448795.112 7393891.101 5531575.537 1261200.45 1648294.352 3165416.986 1957839.32 2827092.883 1717731.485 Fructose 6-phosphate_13C3 0 0 0 0 0 0 0 6195207.923 1318389.281 469220.3018 1213315.381 8324056.172 2168456.44 3800931.23 353755.2407 393448.0389 1157519.098 2033539.804 1376670.026 677582.4438 927390.3263 Fructose 6-phosphate_13C4 2262.208641 0 0 0 0 0 0 1156665.969 201194.0188 37378.16862 196553.0787 1306464.336 335854.5933 519829.6331 0 10761.40981 134742.1212 248567.8236 170621.9908 37731.41291 67859.3216 Fructose 6-phosphate_13C5 0 13155.81638 16164.43722 13993.24437 15895.49399 16041.62161 11222.73378 959435.548 132382.3887 37202.5332 93768.40798 796905.6391 173958.5322 206486.3718 0 3212.54963 57012.16862 130903.2934 97925.26185 6994.72331 40970.64608 Fructose 6-phosphate 92667.43798 1942.167314 4517.871838 4674.018803 1570.209338 4390.067743 7132.878554 34908610.74 21672938.98 8132042.595 42214040.98 148172757.9 40270093.39 359065550.2 315972530.9 67547177.75 81098609.18 142271127.5 93240580.19 149977493.8 86770272.11 Fumaric acid 405835833.1 205275396.8 90939643.7 33193577.21 3517595.677 1500535.795 828242.1348 150207638.6 132931471.8 91657660.57 109102348.2 131037250.8 135165321.3 287278943.7 214977420.7 51911699.22 68402170.94 72758882.94 51142414.91 77119105.87 43648709.69 Fumaric acid_13C1 18130156.51 9058112.544 3975167.192 1504917.638 139275.3129 18568.48131 7910.926634 33013689.86 27156916.61 14540200.22 22391530.19 22679857.3 27596887.39 44237701.3 14180439.7 7683494.402 13446787.69 12590609.88 9880899.735 8022868.921 7479981.519 Fumaric acid_13C2 3564482.283 1839589.026 811557.53 284539.0409 10788.95391 0 0 23905345.83 21082186.75 12875603.03 10558329.06 9228518.127 14939914.71 14291361.28 3132822.769 2610667.079 4996052.693 4934244.847 3677177.81 2212323.004 2690876.116 Fumaric acid_13C3 44898.76826 19019.48688 15515.44661 4160.845929 0 3316.266375 4807.477894 28848241.47 22545045.25 17592968.06 8312136.765 5736761.783 11354880.93 10960014.55 893610.3774 1794255.573 4579501.21 2978432.943 3151194.105 1291298.151 2248934.521 Fumaric acid_13C4 0 0 0 0 0 0 0 35014638.69 35369541.88 28651691.16 9901046.345 5636333.793 19550695.7 15017994.01 1583706.377 2349858.855 5998015.744 4865601.419 3278766.052 1455849.078 2733332.528 Glucose 1-phosphate_13C1 0 0 0 0 0 0 0 5516146.638 2518678.343 918422.807 4199034.332 16058534.04 4601282.775 28484699.37 23232628.98 5258905.781 6934466.961 12324955.11 7870137.809 11738089.57 7242955.675 Glucose 1-phosphate_13C2 0 0 0 0 0 0 0 3581214.583 909627.606 313827.3653 1143698.233 6222760.864 1620005.741 7247525.053 6227611.937 1270571.736 1747751.045 3203660.776 2029603.582 3021851.082 1818721.888 Glucose 1-phosphate_13C3 0 0 0 0 621.2333575 0 0 7280662.982 1399099.794 498279.9753 1328840.42 9753242.105 2500679.204 4231425.132 353755.2407 433104.0652 1310231.227 2198392.838 1451043.434 689021.9085 1029233.186 Glucose 1-phosphate_13C4 0 0 0 0 0 0 0 1283308.478 201194.0188 37378.16862 196553.0787 1418672.036 335854.5933 519829.6331 0 10761.40981 134742.1212 248567.8236 170621.9908 37731.41291 67859.3216 Glucose 6-phosphate_13C1 0 677.245773 0 0 0 0 0 1222713.693 980801.4033 176340.1953 792765.1337 1390278.544 970321.0699 2029229.366 1945569.256 616396.3716 846020.5525 1325600.694 926495.8722 1051490.35 747159.8664 Glucose 6-phosphate_13C2 0 0 0 0 0 0 0 340867.1116 233676.5657 45165.49557 221372.1739 408515.4191 136704.2231 694077.9107 625271.158 91632.61352 155546.4466 328444.8743 128657.0468 274982.6903 149586.3393 Galactaric acid 0 0 0 0 0 0 0 21354150.03 16501736.37 17755333.8 12491792.3 27270632.31 16340499.27 71736009.18 76500349 11742123.48 11800669.73 41700114.55 16981703.91 25751937.22 10970604.5 Galactitol 10960599.53 6287727.666 3396518.754 1723873.215 365649.0786 221453.0019 362397.9497 1095759.757 1195236.715 2144997.234 1368613.819 871207.9694 4900439.92 1506069.895 1139926.118 516684.6127 586965.8965 702923.4849 640496.5546 689879.1897 446573.6516 Galactose 111521.3095 60427.68636 113686.4551 118998.5148 162166.8365 193099.9708 200056.7583 13762223.84 9808730.992 14114646.07 14026999.17 17268313.11 29534941.68 55968437.76 38828830.28 8499545.663 8642226.311 18851240 9873136.938 13649579.69 8129079.952 Galactose 1-phosphate 92667.43798 1942.167314 4517.871838 4674.018803 1570.209338 4390.067743 7132.878554 42076616.04 26583630.09 9350364.739 49462315.16 157237276.1 46598841.77 380347806.8 336324462.3 73391156.37 87865646.05 155018934.3 101273436.1 164496094.1 93545349.75 Galacturonic acid 12344.31096 0 0 6673.737059 8102.049496 32157.45692 5099.703556 20424526.92 18553227.47 13686638.66 17799757.72 33889542.36 27057132.38 151019347.4 175904454.9 51028413.59 38855214.68 83468356.92 42920955.36 79272766.84 28701594.46 gamma-Aminobutyric acid 390888.2383 135787.9114 0 92818.43489 0 0 44546.93621 91554330.44 67785030.67 34058244.59 47285593.74 23241252.57 63961747.4 59300312.38 47647547.61 14078976.84 16458437.75 16465392.01 15498828.97 18047516.25 10286841.06 Gluconic acid 404924.7561 70985.79518 30604.99047 45828.76902 37485.3827 24368.79834 14622.90523 309315790.8 196205618 169078809.5 358233074.9 589277988.6 282480357.1 2156487472 2249521914 817284454.3 780808036.9 1062592597 790555685 1219438732 726647200.2 Glucose 1-phosphate 92667.43798 6449.798218 4517.871838 4674.018803 1570.209338 4390.067743 7132.878554 42140477.52 26583630.09 9407213.526 49815604.65 158260549.8 46800340.08 374938594.6 337581303.4 72804771.68 88110988.15 155809694.9 102700841.7 168022992 94758546.49 Glucose 6-phosphate 10613.29325 3619.900717 867.5543597 5008.468571 909.6600306 880.7247508 816.8621573 21885403.1 16246649.89 3693731.001 15145151.53 23060827.77 15658993.4 35660017.56 30390746.26 8811726.987 11463130.37 19164287.83 11475813.65 14654827.14 11037025.47 Glucose_13C1 7170.348343 0 0 4789.975297 0 5602.604123 0 7843632.222 4484405.331 2168971.217 6592778.747 18895389.78 7018843.009 56395173.45 37606677.2 9771227.263 11416845.31 21042091.29 15960556.75 17552699.93 14411213.18 Glucose_13C2 15943.22059 0 13857.42956 8864.449767 6635.94808 9399.668441 15177.66235 4103906.309 2001650.143 1096729.637 1996554.804 6785641.989 2580991.008 10279206.43 5500367.251 1345063.51 2535639.111 4434891.632 2931544.096 2499056.828 2796991.763 Glucose_13C3 0 0 0 0 0 0 0 9329285.674 3753101.874 1970267.003 3424988.232 12387372.75 4976471.001 11182072.79 506609.0043 1695994.169 5265641.871 7815706.563 4861345.646 1543852.655 3538598.948 Glucose_13C5 0 0 0 5536.633371 0 0 0 1089058.737 436044.3258 239492.9452 281020.4006 1187378.702 396869.6537 289773.3448 0 27176.86624 287263.3766 439949.3944 443702.5665 0 127931.8657 Glucose_13C6 24626.92808 32218.46322 0 54152.80242 9537.481139 27821.2083 19559.42562 1416453.38 424649.1451 127620.6843 218260.9553 1069491.083 479811.9719 879354.4382 53701.15436 101781.4707 376003.6461 558883.7352 537580.1647 19342.99278 248231.095 Glucuronic acid 0 0 0 6673.737059 0 32157.45692 5099.703556 20424526.92 18553227.47 13686638.66 17799757.72 33889542.36 27057132.38 151019347.4 176820084.9 51028413.59 38855214.68 83546606.93 42920955.36 79272766.84 28701594.46 Glutamic acid 1287750.806 518600.7613 324826.3356 327169.3953 210415.4831 355770.7034 208306.1521 1595408211 1177365843 549862439.1 786954902.1 386388569.2 1127773379 908323874.7 833337301.6 229119702.6 262027653.8 269442792.3 260007848.5 302281601.6 176173536.3 Glutamic acid_13C1 17245.51517 8474.389082 6682.507168 4231.213763 0 6329.474203 7482.868802 188936459.3 148846319.4 47191794.43 98781541.31 43568358.44 149829495.2 95168963.3 58364917.53 24980760.17 29678863.72 32122761.29 31831174.5 25480968.31 17882484.63 Glutamic acid_13C2 130096.6599 532586.4451 207477.148 153194.7142 111272.9655 79630.21778 605404.7868 121123993.7 96816881.36 30543226.02 50928051.38 16747233.47 75367587.24 35508321.09 11914395.59 9204735.585 12396884.8 12617904.3 13406114.78 7187028.52 6531722.083 Glutamic acid_13C3 8083.374683 15744.07992 11097.81687 19024.00732 8592.128527 8744.110737 70522.12994 198168094.2 158845457.4 53694453.39 59066510.99 15987240.26 96849794.46 29704111.98 6071348.501 7127892.514 12645284.93 12228800.91 12040292.86 5246839.76 6644975.098 Glutamic acid_13C4 32885.94096 16777.29504 21165.06483 6580.694925 0 12915.82022 11401.56497 42989075.67 32951771.37 16953528.42 10104440.17 2694832.58 16797903.4 6748624.198 1039390.098 1639695.87 3152244.364 2618510.277 2717916.434 908058.8094 1425544.879 Glutamic acid_13C5 1182503.762 1083240.012 559893.1892 607513.1527 1143975.006 746248.9089 837455.435 855024668.8 629422341.4 349362490.2 164538653.4 46797741.14 299542230.7 126310456.7 25660656.91 27511337.8 62334972.82 46359549.29 48112059.53 18901206.84 26527389.69 Glutamic acid_15N1 0 0 0 0 0 4294.951242 0 700588339.7 548284482.9 252808653.4 197954163.2 76630630.97 388248231.6 161001836.9 43615858.69 34886629.12 81028171.93 62685077.57 59311295.75 26493696.28 35447300.02 Glutamine 6063901.886 1630888.758 3027982.962 3440136.838 4163424.012 6583649.405 3288485.394 4179477306 4210812099 4128418851 2653219650 2628875158 3618355825 3717081025 9496407976 2705759203 1751880520 2355259396 2937281778 5389302685 2884313118 Glutaric acid 122675.7186 426062.5066 71499.80853 41971.47829 23893.25621 44834.35114 156771.8487 282456416.8 215099053.3 149105674.7 148443756.7 23434293.4 140679132.4 20500293.32 21703314.14 3791384.899 4201513.617 6870473.9 5699833.467 6649700.707 3734457.693 Glyceric acid 196266.7904 70225.36496 55661.30036 51515.40331 45453.30547 15778.23681 29584.41551 174943999.2 170241775.3 127119538.9 131313284.8 112810780.3 148395723.6 485938929 322548932.9 57156082.61 103499085.6 133601016.8 46507274.16 72192024.84 67405665.69 Glycerol 1055056.739 546318.9364 544836.7927 682716.8531 329261.6283 677881.4076 650528.9074 23362588.14 28216839.37 12250059.21 26431515.77 16607238.26 39396920.26 95702768.23 65044634.47 14822450.09 20601291.2 22892071.79 13235736.73 29658427.72 13166920.09 sn-Glycero-3-phosphate_13C1 509759.2508 596513.5448 401816.9316 123265.3404 0 0 0 13490865.9 8869957.747 1105641.62 16951517.97 31944841.34 13323205.99 67167191.62 41034902.84 14048051.76 17060562.96 21455019.16 16235822.28 22449151.38 13000590.93 sn-Glycero-3-phosphate_13C2 27465.45842 39301.54124 0 0 0 0 0 12640332.05 7131805.094 4960192.61 6873461.626 14600456.01 6430456.882 20812409.11 13752005.52 4004240.272 6288385.893 7800164.019 6164299.472 8147489.672 4770938.267 sn-Glycero-3-phosphate_13C3 0 0 0 0 0 0 0 19635261.06 9040601.839 1072460.268 7648218.049 20854239.95 9641906.125 16762505.27 650793.1905 2296158.942 6334786.376 5677536.309 5036892.034 2497695.367 3001527.585 sn-Glycero-3-phosphate 114211.0951 7074.967178 4467.627979 19742.00616 5385.605893 9125.877696 0 152723052.5 126929415.6 14772755.93 327936742.9 464862581 209480304 1546032517 1162055229 350445583.4 351745788.9 464117064.1 372079393.7 593932251.7 302405087.9 Glycocholic acid 0 0 0 0 0 0 0 4577232.399 4265524.301 761588.9668 576905.9763 823982.4219 3558667.502 9977206.796 376121.1276 248747.4056 187415.3388 153800.1398 279721.3836 271849.8853 238721.0623 Guanidoacetic acid 1138413.62 604528.4124 677378.6305 640666.7566 980052.7026 710570.91 795888.4506 924331891.2 650861342.9 835075486.2 700218650.8 314847545.7 726462270.5 292531561 366641116.5 195934107.7 182603079.6 272391946.8 170836973.1 289042772.5 137563964.8 Guanine 91378.89203 72604.46952 13394.21802 11154.72672 0 23554.89463 28109.45796 191672006.6 151359226.5 293176645.8 300735307.7 114447194 241728421.3 629555006.7 621765124.3 144050868.7 190841055.7 201484135.5 112341010.1 187619902.6 147324332.5 Guanosine 5256.107423 4220.326225 2572.581661 4918.543327 3283.662247 3139.87567 0 158566093.4 117585642.3 260193578.7 257022341 73259292.06 210908777.3 707427729.5 636588678.3 89268918.14 122664848.1 148693685 70911769.85 140510702.9 99241883.99 GDP 3206.911196 1053.144468 0 0 0 0 0 3555598.991 1210712.794 7910.782157 2009.598013 32484848.19 69533.51188 997952.8832 1291722.586 2559221.412 6351540.786 16449619.86 16547174.82 9247757.887 6866462.987 GDP-mannose 0 0 0 0 0 0 0 5054370.5 3482529.971 1407632.538 4492405.209 7255326.256 4061659.105 29299391.89 23190498.15 6376027.778 7053479.648 11395291.2 8025689.313 11353039.91 6868339.075 GMP 0 0 0 0 0 0 0 189622968.8 145066213.8 5264718.282 126745748.7 217796987.9 144609779.8 655553428.9 538634934.1 139822536.6 152007183.8 252163244.3 145857686.7 224655915.6 141939502 GTP 0 0 0 0 0 0 0 68721.48906 44683.34737 0 0 10403270.76 34156.78742 8461.258889 41557.87689 501173.8181 817929.6805 5097640.581 7925697.332 2152026.715 2451474.018 Homovanillic acid 45002.25286 82469.51962 41162.94047 32745.50652 55669.12074 43865.24445 62779.65216 33009062.27 44596799.48 136464264.6 20574730.5 17715819.36 24222556.06 38397431.87 8587486.787 14115908.36 12716916.24 27772161.82 19998308.11 33611117.66 16212256.45 Hydroxyphenyllactic acid 45002.25286 82469.51962 41162.94047 32745.50652 73956.63505 43865.24445 62779.65216 35729634.47 49126102.09 148468458.4 22236714.36 19268871.61 26528980.78 42499602.42 8928248.529 14992238.22 13892668.69 30189317.85 21859947.76 36888200.84 17391813.21 Hydroxypyruvic acid 0 4672.123066 0 0 0 0 0 58185014.19 49371697.8 41032450.19 5153723.979 39100358.03 16686329.84 145230808.2 218986294.7 4614238.412 1955058.692 95703176.74 29109024.31 5998406.613 5357426.34 Indoxyl sulfate 0 0 0 0 0 0 0 115832.4548 471426.4331 4746122.509 190182.0413 1585182.692 1108348.628 246916.5547 61089.39777 306300.9339 290612.0895 320243.6185 462484.1756 276134.8005 291076.8024 IMP 0 0 0 0 0 0 0 9017022.095 24723634.16 763950.1763 16949946.03 44358914.25 43660277.88 163768309.7 168451281.8 30242458.72 26591028.83 36640828.59 37399759.18 79102370.62 13856895.95 Isocitric acid 914212406.6 365009677.1 99458965.65 26999725.52 4170214.049 1346290.255 979353.1003 302563387.2 254133259.9 360369500.2 229522442.3 338800130.2 350629065.6 531947576.7 569085279.1 144451660.4 162536122.7 293310689.3 169630098.2 187328912.6 147278514.4 Isocitric acid_13C1 60071029.82 23731706.09 6371795.218 1637432.773 153773.9442 39630.95511 4950.988614 56779495.99 46209203.08 49136200.82 45183468.05 64689452.08 72775255.16 88442750.83 55763113.52 21265927.74 30648853.54 58663731.78 33994801.61 24255187.17 26286847.28 Isocitric acid_13C2 12491477.45 4847432.03 1215186.082 274670.7984 0 4304.546177 0 27263878.98 23826115.37 22103922.32 17138380.9 19000405.06 31564807.66 28415825.41 10529533.86 5975964.043 10617132.63 20995869.35 11853913.48 5511623.128 8408511.755 Isocitric acid_13C3 772772.4182 247122.9995 32186.19149 0 0 0 0 24954512.16 19380786.79 23682977.49 11557511.15 14903985.7 21804523.24 16502092.68 3963190.163 2979783.91 6519854.33 12792172.11 7034279.104 3431813.434 5112499.087 Isocitric acid_13C4 0 0 0 4638.524646 0 0 0 29533017.64 27403816.07 31659025.91 13918529.4 11461184.88 26555682.37 19987779.68 6149659.422 3949230.135 8368728.112 15425986.45 7085532.896 5303940.395 6578678.749 Isocitric acid_13C5 0 0 0 0 0 0 0 43328053.4 20582313.37 25383819.31 14008548.34 19570573.47 25762396.53 12155472.61 4903497.193 3470918.821 7149187.841 16197671.14 8797990.862 2770167.431 5712388.075 Isocitric acid_13C6 0 0 0 0 0 0 0 7564380.842 3831978.963 3296047.784 2134967.412 2472786.955 4300209.11 1252687.705 62915.24593 530044.2675 1112443.345 1865176.974 1570937.588 215108.8709 703376.9705 Itaconic acid 278248.058 197640.6894 85395.75967 123953.001 100789.8276 28223.32838 35396.07529 122730263.7 147695429.6 77824176.31 83891019.55 59216790.7 92493576.02 82536641.52 50109442.36 57234487.24 49437813.3 56474896.48 52673158.01 72875656.62 48430099.17 Lactic acid 8884961.285 5638007.945 5451841.945 5517749.633 1769069.32 1515130.197 2916232.193 1695475627 1486083841 1036296637 2419245371 3593271268 2091388099 14417755987 9651837676 2446135866 2304625996 4152114473 2871519272 4949934132 2960759702 Lactic acid_13C1 490292.6844 367264.1115 404882.9388 332858.0595 247034.3727 76381.6018 105784.8407 141537005.5 117298008.5 56705460.62 166832408 260105376.1 167390240.2 764281952.2 383476018.4 126370176.7 147340770.9 258530371.4 160682891 219666395.8 176516226.9 Lactic acid_13C2 540911.176 809016.7402 1502556.24 2073156.174 4203207.383 1981702.863 789690.1333 103904478.5 77254138.7 29798174.67 71636322.93 125451853.3 82138481.27 275072051.5 81257320.26 34020225.25 54156328.06 92156433 56571469.8 55816160.16 64546236.03 Lactic acid_13C3 37121.21934 158218.2982 106404.6865 40053.02593 127972.6878 67332.49563 107059.3848 228657139.2 166007066.4 66688623.22 118228337.5 200716219 165123469.3 322605838.1 24963460.76 39194856.39 84854477.62 111101684.1 79720117.57 47147573.09 83753997.69 Lactose 36789888.65 570373.1672 1203051.285 161377.7772 55593.03738 87213.85977 121029.0321 2969939.572 2635175.742 1456153.056 3146288.669 32049437.01 3778674.019 500805513.8 76887266.17 16523301.82 11594247.47 154983378.2 13258606.93 37680453.3 34064270.96 Malic acid 1644139984 905814008.2 412379843.8 161371627.4 18424073.72 8733180.319 4384403.017 1565928944 1374358626 1061048583 1230360015 1401089938 1436330114 2333517952 1963744851 633026984.6 786798640.3 760428158 613475107.2 878652819.6 512956174.6 Malic acid_13C1 75211862.26 41093972.39 18594490.32 7299628.929 753740.6022 336340.7054 152854.7196 326189325.9 272939078.4 157401779.5 250116784.5 239459265.3 288005047.8 335613602.4 133854135 96130362.63 149339149.7 131384902.5 112794444.6 89669763.58 85614746.3 Malic acid_13C2 19170517.67 10276947.28 4693187.054 1785791.738 145174.3254 35897.30116 0 265976371.9 235747340.7 157104782.5 133560285.6 104292433.9 178937322.8 122071813.5 33938756.67 34965193.98 61034242.25 55634104.99 48634787.34 29611557.43 35861226.05 Malic acid_13C3 798070.5837 408156.3859 192113.1954 41658.96519 0 0 0 288235831.1 224877814.3 198817607.5 94888760.41 66305062.59 124597163.9 88058350.98 10323887.76 24472226.75 53274485.56 36021928.69 37620628.79 17253007.38 27810813.29 Malic acid_13C4 48091.47307 26416.82466 56619.43383 26655.43263 40847.1542 65991.88783 31013.11877 335499704.3 347934055.5 317667133.6 116737996.7 64168512.24 197605354.1 113008498.9 13764275.81 28578149.04 64046772.24 48960535.53 38356002.64 19951149.06 30547338.34 Maleic acid 414509464.4 212693426.1 93348475.15 34453129.13 3623364.237 1569836.247 964601.8531 158651219.3 139903794.7 94898055.23 111899368.9 133801511.5 140468318.2 303279344.8 224399037.5 53943540.63 72637107.36 75444041.59 53737841.58 79485460.13 46481455.38 Malonic acid 346650.9763 428425.6019 330492.6741 406240.4283 413441.1451 314393.7867 315339.7222 2156341.939 2405451.807 1967514.987 1523304.572 2928256.323 1370531.027 5427112.681 8145404.048 2151139.875 1449031.531 4217519.611 1927261.58 3188324.247 1461386.424 Maltose 0 618426.3847 1276286.721 175709.0893 205669.986 129627.6974 0 3023066.449 2630275.832 1463635.431 3109940.286 32040917.39 3754158.688 500805513.8 76918550.91 16522248.3 11599662.1 154983378.2 13270303.3 37594535.6 34062310.47 Mannitol 10450784.34 6001751.891 3321646.29 1693018.312 348622.9698 182948.3988 339378.3855 1091964.824 1116274.809 2025579.001 1366248.963 868756.4848 4708877.824 1506069.895 1139926.118 514657.8364 582837.1379 702923.4849 607886.1598 687312.4084 446573.6516 Mannose 0 233723.661 169874.616 88082.40955 64949.91026 86453.18635 63552.44102 63908245.54 35802383.89 21823241.77 58956053.51 120252446.8 61895072.8 646270131.7 505545593.7 118150667.3 140722520.1 216358646.8 174951250.7 230128260.9 173612134.1 Mannose 6-phosphate 92667.43798 1942.167314 4517.871838 4674.018803 1570.209338 4390.067743 7132.878554 34908610.74 21672938.98 8132042.595 42214040.98 148172757.9 40270093.39 359065550.2 315972530.9 67547177.75 81098609.18 142271127.5 93240580.19 149977493.8 86770272.11 Melibiose 19199851.21 9755950.787 4547095.959 2497257.393 848755.1831 719069.4431 4309978.441 5356052.601 2170672.319 4453717.5 2906514.412 6462141.138 3303203.226 112682.7808 0 12294837.66 1725044.823 0 1812648.594 0 0 Mesoxalic acid 8651.918689 16655.52101 16325.41826 4098.263421 4578.311443 4911.410104 0 549016.8029 536771.0579 415525.5815 636388.6477 820015.1003 781353.7039 1832383.175 507547.8188 865465.615 1241586.26 830150.7602 735107.6686 1920438.735 902807.0249 Methyl beta-D-galactoside 144662.9281 47632.39095 96716.77957 114475.412 129835.1562 47074.22609 35125.28335 45597058.19 28159109.14 20312470.27 26923277.21 35830029.95 37750638.64 84784627.71 80233968.19 19323441.02 22481096.75 35468881.13 17079133.27 25550123.34 17025319.3 Methylmalonic acid 317435936.4 151459140.1 64320449.28 24752731.8 2585170.377 1160567.971 851257.4169 343770781.8 352413323.8 274776108.5 368480136.7 470597482.5 216203295.4 1332545940 936334303.5 203216541.2 271413328.5 309109566.3 216644557.6 362497205.4 191866086.7 Methylmalonic acid_13C4 0 0 0 0 0 0 0 156051673.1 151240857.8 121104427.5 53702277.85 28786502.9 53777212.57 78413387.14 8370973.437 11659832.28 34220438.57 30747009.14 22055418.29 10122942.24 18025769.28 Myo-inositol 304265.0494 152953.8468 110407.3745 124997.7869 47311.31745 118386.1257 191889.1604 63908245.54 37664896.57 22201702.45 61099564.67 123690814.2 61931483.23 656950940.7 505490435.5 118122311.6 124816884.1 216237730.9 173218291.9 230135738.9 170474401.5 N-Acetylalanine 132687.2565 49018.52743 105656.4492 0 0 56144.50428 67064.85508 11137596.27 13952688.72 9289665.966 7130015.579 3604399.355 10966633.16 15475441.78 14525035.63 2851972.4 3309728.779 5500467.476 3292474.987 6302024.344 2993817.586 N-Acetylaspartic acid 609820.5247 290394.0069 188137.9669 93693.19528 120366.726 207586.3859 90854.48128 106215892.8 87503204.48 85211228.68 101302200.3 41186318.93 156303206.7 58909726.87 50957438.54 10692707.46 18289555.88 23560345.41 11309768.02 16472748.34 10904168.37 N-Acetylglutamic acid 37679774.2 13169660.88 5381522.954 1051844.503 219090.1013 99982.8497 94169.08185 515181694 302724600.7 178533017.2 305531279 271194593.4 304158908.7 411493796.8 313658535.6 153135880.9 115433340.2 163289391.3 111853143.5 207451180.7 114010259.3 N-Acetylglutamic acid_13C2 300449.965 81966.98223 0 0 0 0 11118.83626 7619473.848 4693082.452 1519532.486 8886177.02 4107213.589 7416027.805 7010715.505 3610236.085 7207766.446 3202551.831 6791071.372 5413085.924 5297283.792 4263676.322 N-Acetylglycine 311128.7681 78547.17991 29835.69264 67456.31133 29693.42717 48840.94069 30924.77413 3260327.538 1723187.347 2125393.265 1727161.707 2112897.513 3084639.092 4198264.672 9139818.191 1743203.137 1632678.448 4504214.714 2016324.784 3844189.352 1846434.247 N-Acetylmethionine 6890.203343 0 0 0 0 0 0 18473178.37 22304432.8 11380164.46 8387916.51 1774589.526 42367437.78 19867089.51 6352182.083 3887265.594 3608846.763 7609356.091 4897278.555 7151820.157 4752793.76 N-Acetylserine 258552.9736 92145.64183 113570.7167 41198.47465 21467.73686 39315.28115 38475.23913 47286316.04 42538222.7 27641970.42 25219007.51 11888178.18 35632053.43 22341783.57 39871251.26 12207810.04 8514175.927 16472391.02 13961069 21692855.4 12141328.18 N-Formylmethionine 0 0 0 0 0 0 0 3124546.623 3556005.654 2502327.519 2635283.824 1645820.849 3546566.352 5098151.398 6952886.875 2654571.203 1808273.347 4797623.251 2326126.316 4513602.289 2050275.461 N-Methyl-D-aspartic acid 1287750.806 518600.7613 324826.3356 327169.3953 210415.4831 355770.7034 208306.1521 1595408211 1177365843 549862439.1 786954902.1 386388569.2 1127773379 908324400.4 833337301.6 229119702.6 262027653.8 269431151 260018207 302284613.9 176173536.3 O-Acetylserine 297349.53 94747.76288 128593.2718 73860.96008 25525.33908 15170.42891 44480.4958 51886853.01 46752092.2 29426011.86 26914775.23 13607897.73 38970027.07 25994838.86 46264243.42 13351550.13 9272512.36 18628642.46 15167345.36 23871281.05 13419172.8 Orotic acid 8357734.27 99274.14237 299247.6383 1275505.787 11560.9349 52960.3876 4762.719192 4615391.07 4362552.538 2847419.33 4905604.752 3267111.117 7280225.833 4184672.159 3351049.876 1098013.458 1015114.91 1473823.419 2164204.98 1998386.841 830082.0474 Oxalic acid 824270.0669 576883.0722 487524.4556 485920.4248 517562.2962 472340.7634 401080.4996 10807063.83 7738656.715 10076556.51 4473345.181 10623719.37 6790882.504 57196297.88 63946078.51 3720648.239 4757628.584 16289197.87 4881646.51 6726343.437 4166216.733 Oxoadipic acid 288471.8637 180177.7382 46268.23839 43866.24162 26265.73325 30590.10031 36418.98849 8998150.373 5293070.601 6706628.668 6289922.555 916828.0241 17284703.34 2592968.364 2352463.014 1857172.952 905678.5784 1148289.547 1191765.788 1779930.066 1370922.961 2-Oxoglutaric acid 280701.2153 15627.61551 53766.45528 16021.69195 5399.88231 29231.04399 23178.97111 8785084.861 6980350.901 7746391.894 5749120.272 5901132.13 9930106.148 8156880.391 3061009.7 5304926.341 5617962.533 5043921.835 4699719.266 7906099.746 4901511.304 2-Oxoglutaric acid_13C1 4809.780338 0 0 0 4242.1519 0 0 644268.9702 591710.1599 504384.1054 483546.1174 350081.3231 1039547.54 356802.629 62355.31314 367481.558 317452.5945 246478.4556 337306.8823 456812.1192 282953.9896 2-Oxoglutaric acid_13C2 5419.112957 0 0 0 0 0 0 0 0 0 0 8698.623044 0 0 0 0 0 0 9166.593355 7847.394132 0 2-Oxoglutaric acid_13C3 8641.654732 0 5546.292373 5116.208076 4714.372947 0 0 583752.4549 486102.3515 365474.8019 248994.1548 38390.58034 493846.4748 40691.78835 0 14872.67269 55732.77253 14447.97915 48714.60804 0 20541.9825 2-Oxoglutaric acid_13C4 0 0 0 4879.909485 9882.608731 4650.978901 4307.893293 83945.6827 80530.03666 118238.9442 6644.424487 17315.2914 88413.87199 17066.35892 0 0 0 0 0 0 0 2-Oxoglutaric acid_13C5 11487.62323 13611.283 9661.557398 23029.81809 10050.29287 38057.02999 25907.90399 2885024.06 2234502.482 3009664.423 760872.0512 255895.3264 1924747.645 251233.6176 0 352570.9527 425095.8755 211331.5312 387503.1864 158563.9444 326495.0744 Palatinose 36809458.36 613393.777 1272085.939 182832.0488 58883.2547 187676.9162 0 3009281.623 2638878.661 1453392.086 3101276.03 32049437.01 3799475.716 500805513.8 76915664.21 16522216.26 11599662.1 154965930.9 13268439.58 37544315.57 34021059.85 Valeric acid 982206.0255 526651.0508 186550.9177 0 248740.3315 251031.898 141232.2378 14755233.19 13029425.21 5695959.451 12287534.32 19146922.18 6800798.415 26520222.03 23597527.5 6584798.805 8682692.711 8451668.571 4176899.724 7245995.361 7908273.794 Phosphocreatine 0 0 0 0 0 0 0 4410509.659 4069107.593 3778490.464 3200405.629 5760428.321 3653564.862 6483283.649 3417463.946 4819709.489 4666718.206 5491907.778 5089053.744 5683366.364 4209805.051 Phosphoenolpyruvic acid 34483.88831 3750.096618 0 0 0 0 0 709057.8301 988172.2331 0 0 6881886.299 17417.5132 45002.34159 129247.1038 76359.83259 608947.9689 1186499.85 2573319.617 302591.4053 538366.3421 Phosphoenolpyruvic acid_13C2 340153.3505 179443.3662 85388.80823 39616.44643 17852.16652 4809.441027 13809.77381 7762134.379 11112508.04 6139070.605 14464416.73 11502288.44 11961132.94 23705260.64 46375577.63 11754806.25 5775192.139 21100695.04 9193197.565 20740589.11 4737017.355 Phosphoenolpyruvic acid_13C3 0 0 0 0 0 0 0 288601.0268 404631.6589 252150.1061 533339.775 625757.2137 421319.4159 1020497.487 1551632.06 447068.7612 255818.9861 811421.1037 322724.935 755949.8678 170805.4395 Pipecolic acid 25549.37207 46323.14729 42165.77162 52125.7807 33109.46964 52598.29376 27391.49928 652386.1296 432031.6063 379209.8361 839113.1602 2507591.614 550044.3733 8167343.483 4468833.189 1185082.456 1912806.806 2794275.785 1203723.609 2152066.883 802182.8962 Psicose 8065169.815 175720.1677 274089.0838 60781.89867 28243.64825 48706.84332 33068.1514 593351.4594 537296.9118 319997.2042 1427527.367 3470574.064 1441704.289 65853933.41 6131034.493 1792856.064 626323.0671 20292484.62 523696.2093 2881364.794 3297579.774 Pyroglutamic acid 4843129.553 4985365.488 3921372.571 2901837.046 1668504.078 1548575.608 2160389.918 382533123.1 189215177.2 123287463 143548436.3 189145062.4 291904758.7 367781420.3 521543423 76590690.24 73715745.83 179248411.3 132828143.5 141992335 67022394.58 Pyruvic acid 83400607.86 22129808.64 20536066.86 2903111.344 613520.6094 152034.7738 245894.3397 8902488.13 11227142.49 7877837.48 52488862.87 14191140.39 50257476.03 8537797.841 1070605.652 21609128.71 40033524.03 4416036.025 8344051.178 28846244.74 22168684.57 Pyruvic acid_13C1 2978022.537 708580.379 596250.6138 64499.58306 7304.370979 0 10699.54367 812273.9931 590625.1463 495328.9666 2438774.257 958002.371 3093484.36 199409.9467 32444.77413 858909.9723 1964064.324 300423.1809 377126.0108 1318022.869 1141646.736 Pyruvic acid_13C3 17365.2438 5571.458709 9498.895676 5660.48958 6449.17272 20321.46596 18010.3144 1208476.379 486736.6551 486116.1347 923006.1144 492070.4567 1999040.086 0 7005.873084 189344.0332 776500.5015 8905.75903 22989.19648 94157.05029 254519.3601 Quinic acid 45620.79335 80296.55617 43527.43188 53619.45972 55464.92181 45277.13182 92155.17222 3448434.762 2663037.657 391963.2604 4365197.97 1983117.731 4317466.578 3760551.953 982002.6815 4568061.618 3185209.645 6732365.239 5470462.396 3235253.484 3015077.074 Quinolinic acid 0 0 0 0 4000.687253 0 4042.939159 2003005.208 1503287.626 2035600.499 1002444.678 515175.733 1753151.095 429364.7513 326294.0602 89916.30801 95257.45953 106628.4906 159985.2345 236561.4324 164379.3116 Raffinose 63109.83241 1006.359636 4025.501704 48467.88828 0 1114.341774 1511.790544 849585.4114 705991.2556 2441369.786 1363123.384 30573834.75 1389372.409 521867374 36737045.4 16192764.53 814636.8634 65963211.81 19883343.02 25415651.28 17932071.84 Resorcinol monoacetate Ribitol 184741.6193 30149.89864 83701.94175 20167.61067 149243.0015 53495.65222 63149.89218 2475071.81 2245787.551 2966377.493 3803100.986 2505668.307 3061976.774 6043159.795 5686474.959 2754345.446 2914288.483 2627882.208 2225701.487 2783100.515 2122973.654 Glucaric acid 0 0 0 0 0 0 0 21892991.78 16460280.39 17404517.6 12675611.23 27258314.09 16179431.45 69470717.87 75329208.05 11356357.62 11246549.72 39214878.5 16950983.73 25658741.49 11449008.94 Salicylic acid 0 75145.70882 50313.39074 20056.30648 34966.71435 57015.74221 22458.028 2730759.142 2264981.099 2321773.409 3769027.519 397437.3918 2167437.186 2978577.269 1548256.384 273265.72 718619.3722 521952.3099 431556.2546 513935.5864 336743.1089 Shikimic acid 160261.6696 74214.05508 41125.97496 19778.07155 61049.49443 41942.1552 46978.67615 3132351.148 3022909.501 2588704.594 1143309.13 517208.4844 1549637.902 622798.8237 290572.3544 433401.785 786920.5101 534160.0057 675890.5645 335647.1383 453230.3041 Sorbitol 10450784.34 6001751.891 3321646.29 1693018.312 348622.9698 182948.3988 339378.3855 1091964.824 1116274.809 2025579.001 1366248.963 868756.4848 4708877.824 1506069.895 1139926.118 514657.8364 582837.1379 702923.4849 607886.1598 687312.4084 446573.6516 Sorbose 111521.3095 60427.68636 113686.4551 118998.5148 162166.8365 193099.9708 200056.7583 13762223.84 9808730.992 14114646.07 14026999.17 17268313.11 29534941.68 55968437.76 38828830.28 8499545.663 8642226.311 18851240 9873136.938 13649579.69 8129079.952 Stachyose 1029.43143 0 0 4579.265785 0 0 0 11142181.99 4670742.025 3374525.318 877195.2992 4176866.36 23743674.41 117542869.5 5831693.426 1192699.892 610380.0396 5165469.487 3691747.901 2665211.848 1293232.1 Succinic acid 316231832.6 151866377.5 64290732.42 24713389.45 2964875.419 1628110.323 878699.0735 343778243.1 351523881.9 274149860.7 359643014.5 473056026.8 216214310 1325424713 906753434.5 202929778.2 283143347.4 321686926.4 219362120.3 362497205.4 197038254.4 Succinic acid_13C1 13876031.48 6703325.863 2913692.478 1116116.613 96823.96269 41957.81411 12319.72926 53243946.72 53464138.15 22502020.3 67701171.62 77645143.7 36061234.55 199971499.4 61404315.73 31118363.18 53796417.92 52672151.98 40143610.99 38462846.93 31808169.67 Succinic acid_13C2 2717908.803 1350489.613 556436.9129 151184.5107 13176.04479 65375.35634 92282.40243 58657671.74 58170091.99 27203878.04 42219859.47 35339622.33 28483144.21 72196990.04 15376690.85 11524442.3 23670285.07 24485999.45 17860637.58 11833151.3 14151249.45 Succinic acid_13C3 20382.28941 22600.82061 4696.720783 0 0 3716.031339 0 33413603.43 31945369.03 15381410.05 22786568.94 18927621.19 11820907.92 53656657.27 4582578.908 7132256.77 19309959.72 13412860.54 12584591.36 7000388.51 10227197.04 Succinic acid_13C4 335093.2905 100738.571 75551.9915 186542.8734 0 220613.6032 0 157778610.7 156277362.5 121019888.2 57172101.78 29557783.57 54235756.54 78441632.62 10800608.54 12968703.77 37308154.08 35718719.58 21513226.55 12545542.83 21117107.64 Sucrose 36809458.36 664197.0876 1258324.08 180871.5516 58899.60059 207294.7696 0 3023066.449 2638931.644 1453444.946 3109940.286 32040917.39 3777208.816 500805513.8 76915664.21 16522248.3 11599662.1 154965930.9 13210145.82 37577971.41 34021059.85 D-Tagatose 71195.63633 227443.7702 137081.2645 80365.92535 74312.71445 97299.7661 0 63908245.54 36996206.63 22113680.66 61597390.69 119620194.9 61895489.43 646288506.2 505568869.3 118123444.9 120810639.7 211954974.1 175039464.2 228090722.8 171119282.2 Tartaric acid 1248850.743 310736.3002 97355.33542 18741.27521 8504.141836 9152.808808 0 689446.06 615035.6968 408712.0791 586504.3629 791218.4575 481411.2681 773791.9724 1344793.235 1209740.613 361269.523 535675.4146 396332.1151 602424.4066 274050.7391 Thiamine diphosphate 0 6124.157445 0 0 0 0 0 3043704.359 2285109.005 1877.2788 2056711.745 9734422.515 1744782.95 14599829.11 12283763.68 4629531.75 5289271.407 10098976.39 6600960.919 7408582.6 5177770.584 Threonine 2522564.644 1716338.338 984703.4964 792255.5223 593297.7079 434304.0061 908974.7508 977798389.4 1144486373 1018726198 513809451.4 426156823.2 906460951.1 614093976.1 994957070.1 274413473.5 238812494.3 461825657.7 362148224.8 541837818.8 295533290.1 Thymidine 132944.454 548072.8701 450555.858 200079.4653 98903.03731 69613.51345 179985.6433 13578606.72 8460864.48 10595425.27 3178187.885 3316714.757 5779504.29 11884659.53 8655815.998 2021167.663 2892309.072 3903293.511 1828318.521 3193343.145 1233115.91 dTMP 0 17388.83213 0 0 0 0 0 4733038.928 3504789.191 259023.8399 2452921.067 1543129.038 3206803.339 14025533.62 11088673.61 2032952.5 2613883.821 3687217.482 1562728.114 3009628.218 1568198.272 Trehalose 36789566.18 608677.9261 1299324.966 177277.3154 55548.28838 94796.46387 109292.4109 2998682.837 2635178.354 1463635.431 3144750.726 32065963.86 3778674.019 500805513.8 76915664.21 16523301.82 11594247.47 154994554.1 13271880.64 35477129.44 34077862.63 Uracil 1432251.622 1884560.922 763539.9118 1404763.275 2157822.334 1102895.658 1337101.993 26365124.79 31807756.49 12479787.84 20209029.27 7868397.947 31079075.41 22628845.7 18886884.3 5790343.38 5402306.461 9141337.176 6111694.906 6949381.605 5561465.383 Uric acid 804107.7908 7537.736757 40432.46272 31135.83663 0 0 3916.366337 0 0 0 38952666.73 41501.45239 2407540.108 0 0 17699544.94 22987468.09 0 9358744.797 28531.3849 3767991.341 Ureidopropionic acid 12281.36984 7290.96142 15273.68639 43399.63968 29179.7386 13346.19746 4919.042449 1759388.618 2151007.496 2700230.924 3367737.226 806940.3883 3857130.904 8706493.106 5636048.836 1731122.206 3616236.28 2197709.128 2435494.163 3066128.583 2647740.599 Uridine 184268.8872 74512.16118 69053.82997 78812.6371 42587.92844 66775.70485 67441.14482 89197516.51 71907075.85 184761496.4 134608647 71555912.3 144381563.1 391122446 276592112.3 60646906.49 78295636.17 96065552.24 62356906.13 117114903.5 66116090.68 UDP 0 0 0 0 0 0 0 15279235.4 8022463.803 361769.0362 867060.8218 53837902.77 2594088.638 20345660.62 20321390.15 22483445.7 34017642.24 50361983.39 39930591.08 40412612.78 29280100.14 UDP-glucose 0 0 0 0 0 1492.45479 0 74314781.17 60310736.52 31690323.52 128035575.6 120441288 129589100.5 808971854.9 774027919.7 185530609.7 189939868.2 288457360.8 195845120.1 257027599.4 182437775.1 UDP-glucuronic acid 0 0 0 0 0 0 0 10199359.9 8511507.359 7195544.299 9270598.718 23841886.52 25166913.03 55240598.45 128237249.1 52158337.14 22890589.92 175525351.8 16710637.8 70810887.31 3773010.154 UDP-galactose 0 0 0 0 0 1492.45479 0 74248936.53 60255164.22 31651299.58 127655118.6 119933198.9 129328121.6 806693563.3 770497122.6 185143865.6 189156341.3 287353484.1 195253561.6 256139904.3 181732842.9 UDP-N-acetylglucosamine 0 0 0 1152.964644 1205.57755 2155.256248 1105.711754 105409983.4 65168077.07 62086804.91 93504703.39 108995518.6 96839062.55 405347545.5 396685826.9 68514682.51 88755663.22 116562569 86117736.68 125890573.3 76647785.29 UMP 1248.679827 1238.338864 929.4230292 1019.71414 2863.849609 802.461517 773.2570891 231786379.7 160993655.9 34536963.49 182741809.3 268114262.1 246807730 1210342767 1136148975 256176618.8 253228959.3 449348716.4 271416216.1 432172836 240296800.4 UTP 7778.878786 2097.241084 0 1137.095311 0 0 0 805880.6946 347224.2771 732.2078088 0 20869570.23 54741.70673 763810.4922 2144059.604 5885604.628 6748729.461 22340275.72 20757716.07 10048189 9574398.544 Urocanic acid 284158.4925 511200.5202 369735.5666 355234.906 112292.613 0 204094.7916 3267580.008 3178360.393 3655106.426 3112932.981 7692124.552 4989061.015 1427876.902 1381643.139 2051847.193 1923141.871 1793128.135 1733445.34 1679331.372 1006303.819 Xanthosine 0 1442.745325 1523.417253 809.3341737 697.4127359 888.295082 1464.771936 26418491.22 9173060.94 5990964.602 24865543.15 3323689.435 42023607.02 95029588.37 71060617.79 2060653.479 6698729.15 13669682.48 2666192.502 12712354.65 1388871.041 Xanthurenic acid 0 0 0 0 0 0 0 0 243005.321 0 183843.4094 0 1961729.736 0 0 2269913.527 835081.842 0 1427893.182 1237695.991 952110.6859 Xylitol 184741.6193 30149.89864 83701.94175 20167.61067 149243.0015 53495.65222 63149.89218 2475071.81 2245787.551 2966377.493 3803100.986 2505668.307 3061976.774 6043159.795 5686474.959 2754345.446 2914288.483 2627882.208 2063163.619 2783100.515 2122973.654 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name Retention index Quantified m/z PubChem ID KEGG ID 10-Hydroxydecanoic acid 2.41 187.13397 74300 C02774 2-Deoxy-glucose 8.6 163.0612 108223 C00586 2-Hydroxybutyric acid 5.15 103.04007 11266 C05984 2-Methylglutaric acid 8.67 145.05063 12046 . 3-(2-Hydroxyphenyl)propanoic acid 2.67 165.05572 873 C01198 Tetrahydrocortisol 2.5 365.23335 44725717 C05465 3-Hydroxybenzaldehyde 121.0295 5967 C03067 3-Hydroxybenzoic acid 5.27 137.02442 7420 C00587 3E-Methylglutaconic acid 8.3 143.03498 1551553 . 3-Phosphoglyceric acid 10.8 184.98566 724 C00597 3-Sulfino-alanine 9.06 152.0023 1549098 C00606 P-Aminobenzoic acid 5.97 136.0404 978 C00568 Vanylglycol 3.87 183.06628 10805 C05594 4-Hydroxybenzaldehyde 2.88 121.0295 126 C00633 4-Hydroxybenzoic acid 5.83 137.02442 135 C00156 4-Pyridoxic acid 2.49 182.04588 6723 C00847 5-Hydroxyindoleacetic acid 7.81 190.05097 1826 C05635 6-Hydroxynicotinic acid 5.96 138.01967 72924 C01020 6-Phosphogluconic acid 11.45 275.01736 91493 C00345 Adenosine 4.01 266.08948 60961 C00212 2',3' cyclic AMP 4.44 328.04524 101812 C02353 Adenosine 3',5'-diphosphate 10.71 426.02214 159296 C00054 ADP-ribose 9.21 558.0644 33576 C00301 ATP 10.77 505.98847 5957 C00002 AMP 8.51 346.05581 6083 C00020 Adipic acid 8.89 145.05063 196 C06104 Allantoin 7.75 157.03671 204 C01551 Allose 7.65 179.05611 12285879 C01487 Glucose 8.31 179.05611 5793 C00267 Arabitol 7.02 151.0612 94154 C01904 Vitamin C 4.43 175.02481 54670067 C00072 Aspartic acid 9.45 132.03023 5960 C00049 Aspartic acid_13C1 9.45 133.03359 Aspartic acid_13C2 9.45 134.03694 Aspartic acid_13C3 9.45 135.0403 Aspartic acid_13C4 9.45 136.04365 Aspartic acid_15N1 9.45 133.02727 Azelaic acid 6.79 187.09758 2266 C08261 sn-Glycero-2-phosphate 8.95 171.0064 2526 C02979 Butyric acid 8.6 87.04515 264 C00246 CDP-Ethanolamine 10.1 445.0531 123727 C00570 Cellobiose 9.23 341.10893 10712 C06422 Citramalic acid 9.26 147.0299 441696 C00815 Citric acid 11.35 191.01973 311 C00158 Citric acid_13C1 11.35 192.02308 Citric acid_13C2 11.35 193.02644 Citric acid_13C3 11.35 194.02979 Citric acid_13C4 11.35 195.03315 Citric acid_13C5 11.35 196.0365 Citric acid_13C6 11.35 197.03985 Cortisol 2.38 361.20205 657311 C00735 3',5' cyclic AMP 4.99 328.04524 6076 C00575 CDP 10.91 402.0109 6132 C00112 CMP 9.96 322.04457 6131 C00055 CTP 12.27 481.97723 6176 C00063 Deoxycytidine 5.02 226.08333 13711 C00881 dCMP 9.29 306.04966 13945 C00239 Deoxyuridine 3.92 227.06734 13712 C00526 Glucuronolactone 5.3 175.02481 439782 C02670 Diacetyl 9.49 85.0295 650 C00741 4,5-Dihydroorotic acid 6.42 157.02548 648 C00337 Dihydroxyacetone phosphate 9.58 168.99075 668 C00111 Ribose 5-phosphate 9.89 229.01188 440101 C00117 beta-D-sedoheptulose 7.78 209.06668 441483 C08355 Ethylmalonic acid 8.39 131.03498 11756 NA Fructose 6-phosphate_13C1 10.05 260.0258 Fructose 6-phosphate_13C2 10.05 261.02915 Fructose 6-phosphate_13C3 10.05 262.03251 Fructose 6-phosphate_13C4 10.05 263.03586 Fructose 6-phosphate_13C5 10.05 264.03922 Fructose 6-phosphate 10.05 259.02244 69507 C00085 Fumaric acid 10.15 115.00368 444972 C00122 Fumaric acid_13C1 10.15 116.00704 Fumaric acid_13C2 10.15 117.01039 Fumaric acid_13C3 10.15 118.01375 Fumaric acid_13C4 10.15 119.0171 Glucose 1-phosphate_13C1 9.94 260.0258 Glucose 1-phosphate_13C2 9.94 261.02915 Glucose 1-phosphate_13C3 9.94 262.03251 Glucose 1-phosphate_13C4 9.94 263.03586 Glucose 6-phosphate_13C1 10.68 260.0258 Glucose 6-phosphate_13C2 10.68 261.02915 Galactaric acid 10.97 209.03029 3037582 C00879 Galactitol 7.88 181.07176 11850 C01697 Galactose 9.69 179.05611 439357 C00984 Galactose 1-phosphate 10.03 259.02244 123912 C00446 Galacturonic acid 10.11 193.03538 84740 C08348 gamma-Aminobutyric acid 8.73 102.05605 223130 C00334 Gluconic acid 8.26 195.05103 10690 C00257 Glucose 1-phosphate 9.94 259.02244 65533 C00103 Glucose 6-phosphate 10.68 259.02244 5958 C00092 Glucose_13C1 8.31 180.05947 Glucose_13C2 8.31 181.06282 Glucose_13C3 8.31 182.06618 Glucose_13C5 8.31 184.07289 Glucose_13C6 8.31 185.07624 Glucuronic acid 9.96 193.03538 94715 C00191 Glutamic acid 9.12 146.04588 33032 C00025 Glutamic acid_13C1 9.12 147.04924 Glutamic acid_13C2 9.12 148.05259 Glutamic acid_13C3 9.12 149.05595 Glutamic acid_13C4 9.12 150.0593 Glutamic acid_13C5 9.12 151.06266 Glutamic acid_15N1 9.12 147.04292 Glutamine 8.48 147.07642 5961 C00064 Glutaric acid 9.23 131.03498 743 C00489 Glyceric acid 6.89 105.01933 439194 C00258 Glycerol 5.23 91.04007 753 C00116 sn-Glycero-3-phosphate_13C1 9.33 172.00975 sn-Glycero-3-phosphate_13C2 9.33 173.01311 sn-Glycero-3-phosphate_13C3 9.33 174.01646 sn-Glycero-3-phosphate 9.33 171.0064 439162 C00093 Glycocholic acid 2.4 466.31631 10140 C01921 Guanidoacetic acid 8.9 118.0611 763 C00581 Guanine 6.34 152.05669 135398634 C00242 Guanosine 6.72 282.08439 135398635 C00387 GDP 11.62 442.01705 135398619 C00035 GDP-mannose 11.72 604.06988 135398627 C00096 GMP 10.56 362.05072 135398631 C00144 GTP 13.11 521.98338 135398633 C00044 Homovanillic acid 4.74 181.05063 1738 C05582 Hydroxyphenyllactic acid 5.04 181.05063 9378 C03672 Hydroxypyruvic acid 6.7 103.00368 964 C00168 Indoxyl sulfate 4.18 212.0023 10258 NA IMP 9.75 347.03982 135398640 C00130 Isocitric acid 12.02 191.01973 1198 C00311 Isocitric acid_13C1 12.02 192.02308 Isocitric acid_13C2 12.02 193.02644 Isocitric acid_13C3 12.02 194.02979 Isocitric acid_13C4 12.02 195.03315 Isocitric acid_13C5 12.02 196.0365 Isocitric acid_13C6 12.02 197.03985 Itaconic acid 9.49 129.01933 811 C00490 Lactic acid 5.36 89.02442 107689 C00186 Lactic acid_13C1 5.36 90.02777 Lactic acid_13C2 5.36 91.03113 Lactic acid_13C3 5.36 92.03448 Lactose 9.24 341.10893 6134 C01970 Malic acid 10.18 133.01425 Malic acid_13C1 10.18 134.0176 Malic acid_13C2 10.18 135.02096 Malic acid_13C3 10.18 136.02431 Malic acid_13C4 10.18 137.02767 Maleic acid 8.67 115.00368 444266 C01384 Malonic acid 10.03 103.00368 867 C00383 Maltose 8.66 341.10893 439341 C00897 Mannitol 7.72 181.07176 6251 C00392 Mannose 8.05 179.05611 18950 C00936 Mannose 6-phosphate 10.17 259.02244 439198 C00275 Melibiose 8.61 341.10893 440658 C05402 Mesoxalic acid 12.27 116.98295 10132 C00830 Methyl beta-D-galactoside 5.87 193.07176 445238 C03619 Methylmalonic acid 9.38 117.01933 487 C02170 Methylmalonic acid_13C4 9.38 121.03275 Myo-inositol 7.65 179.05611 892 C00137 N-Acetylalanine 4.85 130.05097 88064 NA N-Acetylaspartic acid 9.16 174.0408 65065 C01042 N-Acetylglutamic acid 8.78 188.05645 70914 C00624 N-Acetylglutamic acid_13C2 8.78 190.06316 N-Acetylglycine 5.49 116.03532 10972 NA N-Acetylmethionine 2.68 190.05434 6180 C02712 N-Acetylserine 6.04 146.04588 65249 NA N-Formylmethionine 3.55 176.03869 439750 C03145 N-Methyl-D-aspartic acid 7.89 146.04588 22880 C12269 O-Acetylserine 5.94 146.04588 99478 C00979 Orotic acid 5.8 155.00983 967 C00295 Oxalic acid 11.25 88.98803 971 C00209 Oxoadipic acid 9.27 159.0299 71 C00322 2-Oxoglutaric acid 9.84 145.01425 51 C00026 2-Oxoglutaric acid_13C1 9.84 146.0176 2-Oxoglutaric acid_13C2 9.84 147.02096 2-Oxoglutaric acid_13C3 9.84 148.02431 2-Oxoglutaric acid_13C4 9.84 149.02767 2-Oxoglutaric acid_13C5 9.84 150.03102 Palatinose 9.23 341.10893 439559 C017424 Valeric acid 3.01 101.0608 7991 C00803 Phosphocreatine 9.49 210.02853 587 C02305 Phosphoenolpyruvic acid 11.22 166.9751 1005 C00074 Phosphoenolpyruvic acid_13C2 11.22 168.98181 Phosphoenolpyruvic acid_13C3 11.22 169.98516 Pipecolic acid 6.52 128.0717 849 C00408 Psicose 9.16 179.05611 90008 C06468 Pyroglutamic acid 5.9 128.03532 7405 C01879 Pyruvic acid 4.34 87.00877 1060 C00022 Pyruvic acid_13C1 4.34 88.01212 Pyruvic acid_13C3 4.34 90.01883 Quinic acid 7.54 191.05611 6508 C00296 Quinolinic acid 9.7 166.01458 1066 C03722 Raffinose 9.76 503.16176 439242 C00492 Resorcinol monoacetate 2.68 151.04007 5055 C12064 Ribitol 7 151.0612 6912 C00474 Glucaric acid 10.81 209.03029 33037 C00818 Salicylic acid 5.2 137.02442 338 C07588 Shikimic acid 8.29 173.04555 8742 C00493 Sorbitol 7.68 181.07176 5780 C00794 Sorbose 9.69 179.05611 6904 C01452 Stachyose 10.78 665.21458 439531 C01613 Succinic acid 9.62 117.01933 1110 C00042 Succinic acid_13C1 9.62 118.02269 Succinic acid_13C2 9.62 119.02604 Succinic acid_13C3 9.62 120.0294 Succinic acid_13C4 9.62 121.03275 Sucrose 8.61 341.10893 5988 C00089 D-Tagatose 8.69 179.05611 439312 C00795 Tartaric acid 10.93 149.00916 444305 C00898 Thiamine diphosphate 9.08 423.02987 1132 C00068 Threonine 8.22 120.06552 6288 C00188 Thymidine 3.49 241.08299 5789 C00214 dTMP 7.79 321.04933 9700 C00364 Trehalose 9.22 341.10893 7427 C01083 Uracil 3.9 111.02 1174 C00106 Uric acid 7.6 167.02106 1175 C00366 Ureidopropionic acid 6.91 131.04622 111 C02642 Uridine 4.69 243.06226 6029 C00299 UDP 10.55 402.99492 6031 C00015 UDP-glucose 10.4 565.04774 8629 C00029 UDP-glucuronic acid 12.46 579.02701 17473 C00167 UDP-galactose 10.45 565.04774 18068 C00052 UDP-N-acetylglucosamine 9.56 606.07429 445675 C00043 UMP 9.52 323.02859 6030 C00105 UTP 12.02 482.96125 6133 C00075 Urocanic acid 6.2 137.03565 736715 C00785 Xanthosine 7.07 283.06841 64959 C01762 Xanthurenic acid 7.39 206.04478 5699 C02470 Xylitol 7 151.0612 6912 C00379 METABOLITES_END #END