#METABOLOMICS WORKBENCH uchimiya_20240729_191109 DATATRACK_ID:5047 STUDY_ID:ST003363 ANALYSIS_ID:AN005509 PROJECT_ID:PR002089
VERSION                          	1
CREATED_ON                       	08-01-2024
#PROJECT
PR:PROJECT_TITLE                 	Human milk microbiome across lactation stages and free glutamate concentrations
PR:PROJECT_TITLE                 	in healthy Ecuadorian women
PR:PROJECT_SUMMARY               	Project Summary: To examine the differences in microbiome and glutamate
PR:PROJECT_SUMMARY               	concentrations in human breast milk among different lactation stages
PR:PROJECT_SUMMARY               	(colostrum-, transition-, and mature-milk), a longitudinal cohort study for
PR:PROJECT_SUMMARY               	healthy Ecuadorian women was conducted. This deposition is for the glutamate
PR:PROJECT_SUMMARY               	analysis.
PR:INSTITUTE                     	University of Georgia
PR:LAST_NAME                     	Uchimiya
PR:FIRST_NAME                    	Mario
PR:ADDRESS                       	315 Riverbend Rd, Athens, GA, 30602, USA
PR:EMAIL                         	mario.uchimiya@uga.edu
PR:PHONE                         	‭(706) 542-8387‬
PR:FUNDING_SOURCE                	International Glutamate Technical Committee (MEB); Universidad Internacional del
PR:FUNDING_SOURCE                	Ecuador (MEB)
PR:PROJECT_COMMENTS              	This is a deposition for a study led by Manuel E. Baldeon and Lizbeth Peña at
PR:PROJECT_COMMENTS              	the Universidad Internacional del Ecuador (MEB)
PR:DOI                           	http://dx.doi.org/10.21228/M8KR7D
PR:CONTRIBUTORS                  	Manuel E. Baldeon; Lizbeth Peña
#STUDY
ST:STUDY_TITLE                   	Human milk microbiome across lactation stages and free glutamate concentrations
ST:STUDY_TITLE                   	in healthy Ecuadorian women
ST:STUDY_SUMMARY                 	Project Summary: To examine the differences in microbiome and glutamate
ST:STUDY_SUMMARY                 	concentrations in human breast milk among different lactation stages
ST:STUDY_SUMMARY                 	(colostrum-, transition-, and mature-milk), a longitudinal cohort study for
ST:STUDY_SUMMARY                 	healthy Ecuadorian women was conducted. This deposition is for the glutamate
ST:STUDY_SUMMARY                 	analysis.
ST:INSTITUTE                     	Universidad Internacional del Ecuador
ST:LABORATORY                    	Collaboration with the Edison Lab at UGA
ST:LAST_NAME                     	Uchimiya
ST:FIRST_NAME                    	Mario
ST:ADDRESS                       	315 Riverbend Rd, Athens, GA, 30602, USA
ST:EMAIL                         	mario.uchimiya@uga.edu
ST:PHONE                         	‭(706) 542-8387‬
ST:SUBMIT_DATE                   	2024-07-29
#SUBJECT
SU:SUBJECT_TYPE                  	Human
SU:SUBJECT_SPECIES               	Homo sapiens
SU:TAXONOMY_ID                   	9606
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data
SUBJECT_SAMPLE_FACTORS           	Human_brest_milk_study	61-Pool1	Sample source:Human breast milk | Lactation_stage:0 | gender:0	RAW_FILE_NAME(Raw file name)=160; Sample descritpion=Internal_QC-pool
SUBJECT_SAMPLE_FACTORS           	Human_brest_milk_study	62-Pool2	Sample source:Human breast milk | Lactation_stage:0 | gender:0	RAW_FILE_NAME(Raw file name)=154; Sample descritpion=Internal_QC-pool
SUBJECT_SAMPLE_FACTORS           	Human_brest_milk_study	63-Pool3	Sample source:Human breast milk | Lactation_stage:0 | gender:0	RAW_FILE_NAME(Raw file name)=133; Sample descritpion=Internal_QC-pool
SUBJECT_SAMPLE_FACTORS           	Human_brest_milk_study	64-Pool4	Sample source:Human breast milk | Lactation_stage:0 | gender:0	RAW_FILE_NAME(Raw file name)=139; Sample descritpion=Internal_QC-pool
SUBJECT_SAMPLE_FACTORS           	Human_brest_milk_study	65-Pool5	Sample source:Human breast milk | Lactation_stage:0 | gender:0	RAW_FILE_NAME(Raw file name)=132; Sample descritpion=Internal_QC-pool
SUBJECT_SAMPLE_FACTORS           	Human_brest_milk_study	68-External_Control1	Sample source:Human breast milk | Lactation_stage:0 | gender:0	RAW_FILE_NAME(Raw file name)=141; Sample descritpion=External_control
SUBJECT_SAMPLE_FACTORS           	Human_brest_milk_study	69-External_Control2	Sample source:Human breast milk | Lactation_stage:0 | gender:0	RAW_FILE_NAME(Raw file name)=125; Sample descritpion=External_control
SUBJECT_SAMPLE_FACTORS           	Human_brest_milk_study	70-External_Control3	Sample source:Human breast milk | Lactation_stage:0 | gender:0	RAW_FILE_NAME(Raw file name)=150; Sample descritpion=External_control
SUBJECT_SAMPLE_FACTORS           	Human_brest_milk_study	13-BBA_47e_C	Sample source:Human breast milk | Lactation_stage:1 | gender:1	RAW_FILE_NAME(Raw file name)=112; Sample descritpion=Sample_colostrum-milk
SUBJECT_SAMPLE_FACTORS           	Human_brest_milk_study	14-BBA_48e_C	Sample source:Human breast milk | Lactation_stage:1 | gender:1	RAW_FILE_NAME(Raw file name)=148; Sample descritpion=Sample_colostrum-milk
SUBJECT_SAMPLE_FACTORS           	Human_brest_milk_study	16-BBA_60e_C	Sample source:Human breast milk | Lactation_stage:1 | gender:1	RAW_FILE_NAME(Raw file name)=163; Sample descritpion=Sample_colostrum-milk
SUBJECT_SAMPLE_FACTORS           	Human_brest_milk_study	18-BBA_67e_C	Sample source:Human breast milk | Lactation_stage:1 | gender:1	RAW_FILE_NAME(Raw file name)=168; Sample descritpion=Sample_colostrum-milk
SUBJECT_SAMPLE_FACTORS           	Human_brest_milk_study	19-BBA_69e_C	Sample source:Human breast milk | Lactation_stage:1 | gender:1	RAW_FILE_NAME(Raw file name)=136; Sample descritpion=Sample_colostrum-milk
SUBJECT_SAMPLE_FACTORS           	Human_brest_milk_study	1-BBA_10e_C	Sample source:Human breast milk | Lactation_stage:1 | gender:1	RAW_FILE_NAME(Raw file name)=124; Sample descritpion=Sample_colostrum-milk
SUBJECT_SAMPLE_FACTORS           	Human_brest_milk_study	20-BBA_70e_C	Sample source:Human breast milk | Lactation_stage:1 | gender:1	RAW_FILE_NAME(Raw file name)=122; Sample descritpion=Sample_colostrum-milk
SUBJECT_SAMPLE_FACTORS           	Human_brest_milk_study	3-BBA_17e_C	Sample source:Human breast milk | Lactation_stage:1 | gender:1	RAW_FILE_NAME(Raw file name)=134; Sample descritpion=Sample_colostrum-milk
SUBJECT_SAMPLE_FACTORS           	Human_brest_milk_study	5-BBA_19e_C	Sample source:Human breast milk | Lactation_stage:1 | gender:1	RAW_FILE_NAME(Raw file name)=110; Sample descritpion=Sample_colostrum-milk
SUBJECT_SAMPLE_FACTORS           	Human_brest_milk_study	7-BBA_24e_C	Sample source:Human breast milk | Lactation_stage:1 | gender:1	RAW_FILE_NAME(Raw file name)=166; Sample descritpion=Sample_colostrum-milk
SUBJECT_SAMPLE_FACTORS           	Human_brest_milk_study	10-BBA_43e_C	Sample source:Human breast milk | Lactation_stage:1 | gender:2	RAW_FILE_NAME(Raw file name)=159; Sample descritpion=Sample_colostrum-milk
SUBJECT_SAMPLE_FACTORS           	Human_brest_milk_study	11-BBA_44e_C	Sample source:Human breast milk | Lactation_stage:1 | gender:2	RAW_FILE_NAME(Raw file name)=126; Sample descritpion=Sample_colostrum-milk
SUBJECT_SAMPLE_FACTORS           	Human_brest_milk_study	12-BBA_45e_C	Sample source:Human breast milk | Lactation_stage:1 | gender:2	RAW_FILE_NAME(Raw file name)=151; Sample descritpion=Sample_colostrum-milk
SUBJECT_SAMPLE_FACTORS           	Human_brest_milk_study	15-BBA_55e_C	Sample source:Human breast milk | Lactation_stage:1 | gender:2	RAW_FILE_NAME(Raw file name)=156; Sample descritpion=Sample_colostrum-milk
SUBJECT_SAMPLE_FACTORS           	Human_brest_milk_study	17-BBA_61e_C	Sample source:Human breast milk | Lactation_stage:1 | gender:2	RAW_FILE_NAME(Raw file name)=129; Sample descritpion=Sample_colostrum-milk
SUBJECT_SAMPLE_FACTORS           	Human_brest_milk_study	2-BBA_16e_C	Sample source:Human breast milk | Lactation_stage:1 | gender:2	RAW_FILE_NAME(Raw file name)=152; Sample descritpion=Sample_colostrum-milk
SUBJECT_SAMPLE_FACTORS           	Human_brest_milk_study	4-BBA_18e_C	Sample source:Human breast milk | Lactation_stage:1 | gender:2	RAW_FILE_NAME(Raw file name)=116; Sample descritpion=Sample_colostrum-milk
SUBJECT_SAMPLE_FACTORS           	Human_brest_milk_study	6-BBA_22e_C	Sample source:Human breast milk | Lactation_stage:1 | gender:2	RAW_FILE_NAME(Raw file name)=127; Sample descritpion=Sample_colostrum-milk
SUBJECT_SAMPLE_FACTORS           	Human_brest_milk_study	8-BBA_39e_C	Sample source:Human breast milk | Lactation_stage:1 | gender:2	RAW_FILE_NAME(Raw file name)=149; Sample descritpion=Sample_colostrum-milk
SUBJECT_SAMPLE_FACTORS           	Human_brest_milk_study	9-BBA_40e_C	Sample source:Human breast milk | Lactation_stage:1 | gender:2	RAW_FILE_NAME(Raw file name)=111; Sample descritpion=Sample_colostrum-milk
SUBJECT_SAMPLE_FACTORS           	Human_brest_milk_study	21-BBA_10e_T	Sample source:Human breast milk | Lactation_stage:2 | gender:1	RAW_FILE_NAME(Raw file name)=142; Sample descritpion=Sample_transition-milk
SUBJECT_SAMPLE_FACTORS           	Human_brest_milk_study	23-BBA_17e_T	Sample source:Human breast milk | Lactation_stage:2 | gender:1	RAW_FILE_NAME(Raw file name)=157; Sample descritpion=Sample_transition-milk
SUBJECT_SAMPLE_FACTORS           	Human_brest_milk_study	25-BBA_19e_T	Sample source:Human breast milk | Lactation_stage:2 | gender:1	RAW_FILE_NAME(Raw file name)=162; Sample descritpion=Sample_transition-milk
SUBJECT_SAMPLE_FACTORS           	Human_brest_milk_study	27-BBA_24e_T	Sample source:Human breast milk | Lactation_stage:2 | gender:1	RAW_FILE_NAME(Raw file name)=113; Sample descritpion=Sample_transition-milk
SUBJECT_SAMPLE_FACTORS           	Human_brest_milk_study	33-BBA_47e_T	Sample source:Human breast milk | Lactation_stage:2 | gender:1	RAW_FILE_NAME(Raw file name)=123; Sample descritpion=Sample_transition-milk
SUBJECT_SAMPLE_FACTORS           	Human_brest_milk_study	34-BBA_48e_T	Sample source:Human breast milk | Lactation_stage:2 | gender:1	RAW_FILE_NAME(Raw file name)=108; Sample descritpion=Sample_transition-milk
SUBJECT_SAMPLE_FACTORS           	Human_brest_milk_study	36-BBA_60e_T	Sample source:Human breast milk | Lactation_stage:2 | gender:1	RAW_FILE_NAME(Raw file name)=158; Sample descritpion=Sample_transition-milk
SUBJECT_SAMPLE_FACTORS           	Human_brest_milk_study	38-BBA_67e_T	Sample source:Human breast milk | Lactation_stage:2 | gender:1	RAW_FILE_NAME(Raw file name)=130; Sample descritpion=Sample_transition-milk
SUBJECT_SAMPLE_FACTORS           	Human_brest_milk_study	39-BBA_69e_T	Sample source:Human breast milk | Lactation_stage:2 | gender:1	RAW_FILE_NAME(Raw file name)=103; Sample descritpion=Sample_transition-milk
SUBJECT_SAMPLE_FACTORS           	Human_brest_milk_study	40-BBA_70e_T	Sample source:Human breast milk | Lactation_stage:2 | gender:1	RAW_FILE_NAME(Raw file name)=118; Sample descritpion=Sample_transition-milk
SUBJECT_SAMPLE_FACTORS           	Human_brest_milk_study	22-BBA_16e_T	Sample source:Human breast milk | Lactation_stage:2 | gender:2	RAW_FILE_NAME(Raw file name)=131; Sample descritpion=Sample_transition-milk
SUBJECT_SAMPLE_FACTORS           	Human_brest_milk_study	24-BBA_18e_T	Sample source:Human breast milk | Lactation_stage:2 | gender:2	RAW_FILE_NAME(Raw file name)=128; Sample descritpion=Sample_transition-milk
SUBJECT_SAMPLE_FACTORS           	Human_brest_milk_study	26-BBA_22e_T	Sample source:Human breast milk | Lactation_stage:2 | gender:2	RAW_FILE_NAME(Raw file name)=169; Sample descritpion=Sample_transition-milk
SUBJECT_SAMPLE_FACTORS           	Human_brest_milk_study	28-BBA_39e_T	Sample source:Human breast milk | Lactation_stage:2 | gender:2	RAW_FILE_NAME(Raw file name)=117; Sample descritpion=Sample_transition-milk
SUBJECT_SAMPLE_FACTORS           	Human_brest_milk_study	29-BBA_40e_T	Sample source:Human breast milk | Lactation_stage:2 | gender:2	RAW_FILE_NAME(Raw file name)=119; Sample descritpion=Sample_transition-milk
SUBJECT_SAMPLE_FACTORS           	Human_brest_milk_study	30-BBA_43e_T	Sample source:Human breast milk | Lactation_stage:2 | gender:2	RAW_FILE_NAME(Raw file name)=140; Sample descritpion=Sample_transition-milk
SUBJECT_SAMPLE_FACTORS           	Human_brest_milk_study	31-BBA_44e_T	Sample source:Human breast milk | Lactation_stage:2 | gender:2	RAW_FILE_NAME(Raw file name)=137; Sample descritpion=Sample_transition-milk
SUBJECT_SAMPLE_FACTORS           	Human_brest_milk_study	32-BBA_45e_T	Sample source:Human breast milk | Lactation_stage:2 | gender:2	RAW_FILE_NAME(Raw file name)=164; Sample descritpion=Sample_transition-milk
SUBJECT_SAMPLE_FACTORS           	Human_brest_milk_study	35-BBA_55e_T	Sample source:Human breast milk | Lactation_stage:2 | gender:2	RAW_FILE_NAME(Raw file name)=107; Sample descritpion=Sample_transition-milk
SUBJECT_SAMPLE_FACTORS           	Human_brest_milk_study	37-BBA_61e_T	Sample source:Human breast milk | Lactation_stage:2 | gender:2	RAW_FILE_NAME(Raw file name)=165; Sample descritpion=Sample_transition-milk
SUBJECT_SAMPLE_FACTORS           	Human_brest_milk_study	41-BBA_10e_M	Sample source:Human breast milk | Lactation_stage:3 | gender:1	RAW_FILE_NAME(Raw file name)=145; Sample descritpion=Sample_mature-milk
SUBJECT_SAMPLE_FACTORS           	Human_brest_milk_study	43-BBA_17e_M	Sample source:Human breast milk | Lactation_stage:3 | gender:1	RAW_FILE_NAME(Raw file name)=153; Sample descritpion=Sample_mature-milk
SUBJECT_SAMPLE_FACTORS           	Human_brest_milk_study	45-BBA_19e_M	Sample source:Human breast milk | Lactation_stage:3 | gender:1	RAW_FILE_NAME(Raw file name)=167; Sample descritpion=Sample_mature-milk
SUBJECT_SAMPLE_FACTORS           	Human_brest_milk_study	47-BBA_24e_M	Sample source:Human breast milk | Lactation_stage:3 | gender:1	RAW_FILE_NAME(Raw file name)=161; Sample descritpion=Sample_mature-milk
SUBJECT_SAMPLE_FACTORS           	Human_brest_milk_study	53-BBA_47e_M	Sample source:Human breast milk | Lactation_stage:3 | gender:1	RAW_FILE_NAME(Raw file name)=155; Sample descritpion=Sample_mature-milk
SUBJECT_SAMPLE_FACTORS           	Human_brest_milk_study	54-BBA_48e_M	Sample source:Human breast milk | Lactation_stage:3 | gender:1	RAW_FILE_NAME(Raw file name)=146; Sample descritpion=Sample_mature-milk
SUBJECT_SAMPLE_FACTORS           	Human_brest_milk_study	56-BBA_60e_M	Sample source:Human breast milk | Lactation_stage:3 | gender:1	RAW_FILE_NAME(Raw file name)=135; Sample descritpion=Sample_mature-milk
SUBJECT_SAMPLE_FACTORS           	Human_brest_milk_study	58-BBA_67e_M	Sample source:Human breast milk | Lactation_stage:3 | gender:1	RAW_FILE_NAME(Raw file name)=115; Sample descritpion=Sample_mature-milk
SUBJECT_SAMPLE_FACTORS           	Human_brest_milk_study	59-BBA_69e_M	Sample source:Human breast milk | Lactation_stage:3 | gender:1	RAW_FILE_NAME(Raw file name)=109; Sample descritpion=Sample_mature-milk
SUBJECT_SAMPLE_FACTORS           	Human_brest_milk_study	60-BBA_70e_M	Sample source:Human breast milk | Lactation_stage:3 | gender:1	RAW_FILE_NAME(Raw file name)=114; Sample descritpion=Sample_mature-milk
SUBJECT_SAMPLE_FACTORS           	Human_brest_milk_study	42-BBA_16e_M	Sample source:Human breast milk | Lactation_stage:3 | gender:2	RAW_FILE_NAME(Raw file name)=138; Sample descritpion=Sample_mature-milk
SUBJECT_SAMPLE_FACTORS           	Human_brest_milk_study	44-BBA_18e_M	Sample source:Human breast milk | Lactation_stage:3 | gender:2	RAW_FILE_NAME(Raw file name)=170; Sample descritpion=Sample_mature-milk
SUBJECT_SAMPLE_FACTORS           	Human_brest_milk_study	46-BBA_22e_M	Sample source:Human breast milk | Lactation_stage:3 | gender:2	RAW_FILE_NAME(Raw file name)=121; Sample descritpion=Sample_mature-milk
SUBJECT_SAMPLE_FACTORS           	Human_brest_milk_study	48-BBA_39e_M	Sample source:Human breast milk | Lactation_stage:3 | gender:2	RAW_FILE_NAME(Raw file name)=105; Sample descritpion=Sample_mature-milk
SUBJECT_SAMPLE_FACTORS           	Human_brest_milk_study	49-BBA_40e_M	Sample source:Human breast milk | Lactation_stage:3 | gender:2	RAW_FILE_NAME(Raw file name)=120; Sample descritpion=Sample_mature-milk
SUBJECT_SAMPLE_FACTORS           	Human_brest_milk_study	50-BBA_43e_M	Sample source:Human breast milk | Lactation_stage:3 | gender:2	RAW_FILE_NAME(Raw file name)=104; Sample descritpion=Sample_mature-milk
SUBJECT_SAMPLE_FACTORS           	Human_brest_milk_study	51-BBA_44e_M	Sample source:Human breast milk | Lactation_stage:3 | gender:2	RAW_FILE_NAME(Raw file name)=144; Sample descritpion=Sample_mature-milk
SUBJECT_SAMPLE_FACTORS           	Human_brest_milk_study	52-BBA_45e_M	Sample source:Human breast milk | Lactation_stage:3 | gender:2	RAW_FILE_NAME(Raw file name)=147; Sample descritpion=Sample_mature-milk
SUBJECT_SAMPLE_FACTORS           	Human_brest_milk_study	55-BBA_55e_M	Sample source:Human breast milk | Lactation_stage:3 | gender:2	RAW_FILE_NAME(Raw file name)=143; Sample descritpion=Sample_mature-milk
SUBJECT_SAMPLE_FACTORS           	Human_brest_milk_study	57-BBA_61e_M	Sample source:Human breast milk | Lactation_stage:3 | gender:2	RAW_FILE_NAME(Raw file name)=106; Sample descritpion=Sample_mature-milk
SUBJECT_SAMPLE_FACTORS           	Human_brest_milk_study	66-Extr_Blank1	Sample source:n.a. | Lactation_stage:0 | gender:0	RAW_FILE_NAME(Raw file name)=102; Sample descritpion=Extraction blank
SUBJECT_SAMPLE_FACTORS           	Human_brest_milk_study	67-Extr_Blank2	Sample source:n.a. | Lactation_stage:0 | gender:0	RAW_FILE_NAME(Raw file name)=171; Sample descritpion=Extraction blank
SUBJECT_SAMPLE_FACTORS           	Human_brest_milk_study	Buffer phosphate blank	Sample source:n.a. | Lactation_stage:0 | gender:0	RAW_FILE_NAME(Raw file name)=101; Sample descritpion=Buffer blank
#COLLECTION
CO:COLLECTION_SUMMARY            	7.5 to 20 mL of milk samples were collected from mothers and kept in sterile
CO:COLLECTION_SUMMARY            	50-mL tubes (Sigma-Aldrich). The samples were kept cold and transported to the
CO:COLLECTION_SUMMARY            	laboratory within the first two hours of the sampling. 5 mL of the breast milk
CO:COLLECTION_SUMMARY            	samples were aliquoted in cryovials and stored at -80oC for glutamate analysis.
CO:COLLECTION_SUMMARY            	Lactation stage 1 = colostrum, 2 = transition, 3 = mature.
CO:SAMPLE_TYPE                   	Milk
#TREATMENT
TR:TREATMENT_SUMMARY             	Mothers were recruited at Hospital Gineco Obstétrico Isidro Ayora in Quito,
TR:TREATMENT_SUMMARY             	Ecuador. The participating mothers were permanent residents and indicated their
TR:TREATMENT_SUMMARY             	willingness to exclusively breastfeed for at least two months. Mothers were
TR:TREATMENT_SUMMARY             	healthy, ≥18 years of age, and primiparous, and they had not consumed
TR:TREATMENT_SUMMARY             	antibiotics or probiotics during pregnancy. Each mother had given birth to a
TR:TREATMENT_SUMMARY             	full-term baby via vaginal delivery. A total of 20 mothers donated their
TR:TREATMENT_SUMMARY             	colostrum- (human milk from 1-7 days postpartum), transition- (15 days
TR:TREATMENT_SUMMARY             	postpartum), and mature-milk (30 days postpartum). For Factor_2 (gender) in
TR:TREATMENT_SUMMARY             	Study Design, 1: participant with a female baby; 2: one with a male baby; 0: not
TR:TREATMENT_SUMMARY             	applicable.
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	Human breast milk samples were extracted using a two-phase extraction method. 2
SP:SAMPLEPREP_SUMMARY            	mL of chloroform-methanol (2:1) was added to each breast milk sample and
SP:SAMPLEPREP_SUMMARY            	vortexed for 10 seconds. 600 µL of water was then added to each sample and the
SP:SAMPLEPREP_SUMMARY            	mixture was centrifuged. 800 µL of the polar phase from each sample was
SP:SAMPLEPREP_SUMMARY            	transferred into a 1.5mL glass tube. The samples were then dried using a speed
SP:SAMPLEPREP_SUMMARY            	vacuum centrifuge. The polar phase was reconstituted in 700 µL of a phosphate
SP:SAMPLEPREP_SUMMARY            	buffer (pH 7.4; 1M TSP). The samples were transferred to 5-mm NMR tubes for NMR
SP:SAMPLEPREP_SUMMARY            	analysis.
SP:SAMPLEPREP_PROTOCOL_FILENAME  	4_Sample_preparation_protocol_Glu_2024.pdf
SP:PROCESSING_STORAGE_CONDITIONS 	4℃
#CHROMATOGRAPHY
#ANALYSIS
AN:ANALYSIS_TYPE                 	NMR
AN:ANALYSIS_PROTOCOL_FILE        	5_Analysis_protocol_Glu_2024.pdf
#NMR
NM:INSTRUMENT_NAME               	Bruker AVANCE lll
NM:INSTRUMENT_TYPE               	FT-NMR
NM:NMR_EXPERIMENT_TYPE           	2D-1H-1H
NM:NMR_COMMENTS                  	The raw NMR data files are in the 'Spectra' folder.
NM:SPECTROMETER_FREQUENCY        	600
#NMR_METABOLITE_DATA
NMR_METABOLITE_DATA:UNITS        	NMR peak intensity
NMR_METABOLITE_DATA_START
Samples	13-BBA_47e_C	14-BBA_48e_C	16-BBA_60e_C	18-BBA_67e_C	19-BBA_69e_C	1-BBA_10e_C	20-BBA_70e_C	3-BBA_17e_C	5-BBA_19e_C	7-BBA_24e_C	10-BBA_43e_C	11-BBA_44e_C	12-BBA_45e_C	15-BBA_55e_C	17-BBA_61e_C	2-BBA_16e_C	4-BBA_18e_C	6-BBA_22e_C	8-BBA_39e_C	9-BBA_40e_C	21-BBA_10e_T	23-BBA_17e_T	25-BBA_19e_T	27-BBA_24e_T	33-BBA_47e_T	34-BBA_48e_T	36-BBA_60e_T	38-BBA_67e_T	39-BBA_69e_T	40-BBA_70e_T	22-BBA_16e_T	24-BBA_18e_T	26-BBA_22e_T	28-BBA_39e_T	29-BBA_40e_T	30-BBA_43e_T	31-BBA_44e_T	32-BBA_45e_T	35-BBA_55e_T	37-BBA_61e_T	41-BBA_10e_M	43-BBA_17e_M	45-BBA_19e_M	47-BBA_24e_M	53-BBA_47e_M	54-BBA_48e_M	56-BBA_60e_M	58-BBA_67e_M	59-BBA_69e_M	60-BBA_70e_M	42-BBA_16e_M	44-BBA_18e_M	46-BBA_22e_M	48-BBA_39e_M	49-BBA_40e_M	50-BBA_43e_M	51-BBA_44e_M	52-BBA_45e_M	55-BBA_55e_M	57-BBA_61e_M
Factors	Sample source:Human breast milk | Lactation_stage:1 | gender:1	Sample source:Human breast milk | Lactation_stage:1 | gender:1	Sample source:Human breast milk | Lactation_stage:1 | gender:1	Sample source:Human breast milk | Lactation_stage:1 | gender:1	Sample source:Human breast milk | Lactation_stage:1 | gender:1	Sample source:Human breast milk | Lactation_stage:1 | gender:1	Sample source:Human breast milk | Lactation_stage:1 | gender:1	Sample source:Human breast milk | Lactation_stage:1 | gender:1	Sample source:Human breast milk | Lactation_stage:1 | gender:1	Sample source:Human breast milk | Lactation_stage:1 | gender:1	Sample source:Human breast milk | Lactation_stage:1 | gender:2	Sample source:Human breast milk | Lactation_stage:1 | gender:2	Sample source:Human breast milk | Lactation_stage:1 | gender:2	Sample source:Human breast milk | Lactation_stage:1 | gender:2	Sample source:Human breast milk | Lactation_stage:1 | gender:2	Sample source:Human breast milk | Lactation_stage:1 | gender:2	Sample source:Human breast milk | Lactation_stage:1 | gender:2	Sample source:Human breast milk | Lactation_stage:1 | gender:2	Sample source:Human breast milk | Lactation_stage:1 | gender:2	Sample source:Human breast milk | Lactation_stage:1 | gender:2	Sample source:Human breast milk | Lactation_stage:2 | gender:1	Sample source:Human breast milk | Lactation_stage:2 | gender:1	Sample source:Human breast milk | Lactation_stage:2 | gender:1	Sample source:Human breast milk | Lactation_stage:2 | gender:1	Sample source:Human breast milk | Lactation_stage:2 | gender:1	Sample source:Human breast milk | Lactation_stage:2 | gender:1	Sample source:Human breast milk | Lactation_stage:2 | gender:1	Sample source:Human breast milk | Lactation_stage:2 | gender:1	Sample source:Human breast milk | Lactation_stage:2 | gender:1	Sample source:Human breast milk | Lactation_stage:2 | gender:1	Sample source:Human breast milk | Lactation_stage:2 | gender:2	Sample source:Human breast milk | Lactation_stage:2 | gender:2	Sample source:Human breast milk | Lactation_stage:2 | gender:2	Sample source:Human breast milk | Lactation_stage:2 | gender:2	Sample source:Human breast milk | Lactation_stage:2 | gender:2	Sample source:Human breast milk | Lactation_stage:2 | gender:2	Sample source:Human breast milk | Lactation_stage:2 | gender:2	Sample source:Human breast milk | Lactation_stage:2 | gender:2	Sample source:Human breast milk | Lactation_stage:2 | gender:2	Sample source:Human breast milk | Lactation_stage:2 | gender:2	Sample source:Human breast milk | Lactation_stage:3 | gender:1	Sample source:Human breast milk | Lactation_stage:3 | gender:1	Sample source:Human breast milk | Lactation_stage:3 | gender:1	Sample source:Human breast milk | Lactation_stage:3 | gender:1	Sample source:Human breast milk | Lactation_stage:3 | gender:1	Sample source:Human breast milk | Lactation_stage:3 | gender:1	Sample source:Human breast milk | Lactation_stage:3 | gender:1	Sample source:Human breast milk | Lactation_stage:3 | gender:1	Sample source:Human breast milk | Lactation_stage:3 | gender:1	Sample source:Human breast milk | Lactation_stage:3 | gender:1	Sample source:Human breast milk | Lactation_stage:3 | gender:2	Sample source:Human breast milk | Lactation_stage:3 | gender:2	Sample source:Human breast milk | Lactation_stage:3 | gender:2	Sample source:Human breast milk | Lactation_stage:3 | gender:2	Sample source:Human breast milk | Lactation_stage:3 | gender:2	Sample source:Human breast milk | Lactation_stage:3 | gender:2	Sample source:Human breast milk | Lactation_stage:3 | gender:2	Sample source:Human breast milk | Lactation_stage:3 | gender:2	Sample source:Human breast milk | Lactation_stage:3 | gender:2	Sample source:Human breast milk | Lactation_stage:3 | gender:2	
Glutamate	0.0075	0.0100	0.0039	0.0052	0.0049	0.0035	0.0071	0.0026	0.0079	0.0109	0.0051	0.0049	0.0054	0.0088	0.0136	0.0036	0.0070	0.0910	0.0072	0.0163	0.0042	0.0096	0.0165	0.0141	0.0108	0.0132	0.0058	0.0136	0.0194	0.0081	0.0048	0.0106	0.0085	0.0138	0.0068	0.0154	0.0173	0.0185	0.0094	0.0130	0.0037	0.0109	0.0174	0.0085	0.0182	0.0146	0.0157	0.0248	0.0155	0.0130	0.0121	0.0125	0.0122	0.0186	0.0126	0.0182	0.0194	0.0155	0.0185	0.0122
NMR_METABOLITE_DATA_END
#METABOLITES
METABOLITES_START
metabolite_name	pubchem_id	inchi_key	kegg_id	other_id	other_id_type	ri	ri_type	moverz_quant	
Glutamate	4525487								
METABOLITES_END
#END