#METABOLOMICS WORKBENCH uchimiya_20240729_191109 DATATRACK_ID:5047 STUDY_ID:ST003363 ANALYSIS_ID:AN005509 PROJECT_ID:PR002089 VERSION 1 CREATED_ON 08-01-2024 #PROJECT PR:PROJECT_TITLE Human milk microbiome across lactation stages and free glutamate concentrations PR:PROJECT_TITLE in healthy Ecuadorian women PR:PROJECT_SUMMARY Project Summary: To examine the differences in microbiome and glutamate PR:PROJECT_SUMMARY concentrations in human breast milk among different lactation stages PR:PROJECT_SUMMARY (colostrum-, transition-, and mature-milk), a longitudinal cohort study for PR:PROJECT_SUMMARY healthy Ecuadorian women was conducted. This deposition is for the glutamate PR:PROJECT_SUMMARY analysis. PR:INSTITUTE University of Georgia PR:LAST_NAME Uchimiya PR:FIRST_NAME Mario PR:ADDRESS 315 Riverbend Rd, Athens, GA, 30602, USA PR:EMAIL mario.uchimiya@uga.edu PR:PHONE (706) 542-8387 PR:FUNDING_SOURCE International Glutamate Technical Committee (MEB); Universidad Internacional del PR:FUNDING_SOURCE Ecuador (MEB) PR:PROJECT_COMMENTS This is a deposition for a study led by Manuel E. Baldeon and Lizbeth Peña at PR:PROJECT_COMMENTS the Universidad Internacional del Ecuador (MEB) PR:DOI http://dx.doi.org/10.21228/M8KR7D PR:CONTRIBUTORS Manuel E. Baldeon; Lizbeth Peña #STUDY ST:STUDY_TITLE Human milk microbiome across lactation stages and free glutamate concentrations ST:STUDY_TITLE in healthy Ecuadorian women ST:STUDY_SUMMARY Project Summary: To examine the differences in microbiome and glutamate ST:STUDY_SUMMARY concentrations in human breast milk among different lactation stages ST:STUDY_SUMMARY (colostrum-, transition-, and mature-milk), a longitudinal cohort study for ST:STUDY_SUMMARY healthy Ecuadorian women was conducted. This deposition is for the glutamate ST:STUDY_SUMMARY analysis. ST:INSTITUTE Universidad Internacional del Ecuador ST:LABORATORY Collaboration with the Edison Lab at UGA ST:LAST_NAME Uchimiya ST:FIRST_NAME Mario ST:ADDRESS 315 Riverbend Rd, Athens, GA, 30602, USA ST:EMAIL mario.uchimiya@uga.edu ST:PHONE (706) 542-8387 ST:SUBMIT_DATE 2024-07-29 #SUBJECT SU:SUBJECT_TYPE Human SU:SUBJECT_SPECIES Homo sapiens SU:TAXONOMY_ID 9606 #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS Human_brest_milk_study 61-Pool1 Sample source:Human breast milk | Lactation_stage:0 | gender:0 RAW_FILE_NAME(Raw file name)=160; Sample descritpion=Internal_QC-pool SUBJECT_SAMPLE_FACTORS Human_brest_milk_study 62-Pool2 Sample source:Human breast milk | Lactation_stage:0 | gender:0 RAW_FILE_NAME(Raw file name)=154; Sample descritpion=Internal_QC-pool SUBJECT_SAMPLE_FACTORS Human_brest_milk_study 63-Pool3 Sample source:Human breast milk | Lactation_stage:0 | gender:0 RAW_FILE_NAME(Raw file name)=133; Sample descritpion=Internal_QC-pool SUBJECT_SAMPLE_FACTORS Human_brest_milk_study 64-Pool4 Sample source:Human breast milk | Lactation_stage:0 | gender:0 RAW_FILE_NAME(Raw file name)=139; Sample descritpion=Internal_QC-pool SUBJECT_SAMPLE_FACTORS Human_brest_milk_study 65-Pool5 Sample source:Human breast milk | Lactation_stage:0 | gender:0 RAW_FILE_NAME(Raw file name)=132; Sample descritpion=Internal_QC-pool SUBJECT_SAMPLE_FACTORS Human_brest_milk_study 68-External_Control1 Sample source:Human breast milk | Lactation_stage:0 | gender:0 RAW_FILE_NAME(Raw file name)=141; Sample descritpion=External_control SUBJECT_SAMPLE_FACTORS Human_brest_milk_study 69-External_Control2 Sample source:Human breast milk | Lactation_stage:0 | gender:0 RAW_FILE_NAME(Raw file name)=125; Sample descritpion=External_control SUBJECT_SAMPLE_FACTORS Human_brest_milk_study 70-External_Control3 Sample source:Human breast milk | Lactation_stage:0 | gender:0 RAW_FILE_NAME(Raw file name)=150; Sample descritpion=External_control SUBJECT_SAMPLE_FACTORS Human_brest_milk_study 13-BBA_47e_C Sample source:Human breast milk | Lactation_stage:1 | gender:1 RAW_FILE_NAME(Raw file name)=112; Sample descritpion=Sample_colostrum-milk SUBJECT_SAMPLE_FACTORS Human_brest_milk_study 14-BBA_48e_C Sample source:Human breast milk | Lactation_stage:1 | gender:1 RAW_FILE_NAME(Raw file name)=148; Sample descritpion=Sample_colostrum-milk SUBJECT_SAMPLE_FACTORS Human_brest_milk_study 16-BBA_60e_C Sample source:Human breast milk | Lactation_stage:1 | gender:1 RAW_FILE_NAME(Raw file name)=163; Sample descritpion=Sample_colostrum-milk SUBJECT_SAMPLE_FACTORS Human_brest_milk_study 18-BBA_67e_C Sample source:Human breast milk | Lactation_stage:1 | gender:1 RAW_FILE_NAME(Raw file name)=168; Sample descritpion=Sample_colostrum-milk SUBJECT_SAMPLE_FACTORS Human_brest_milk_study 19-BBA_69e_C Sample source:Human breast milk | Lactation_stage:1 | gender:1 RAW_FILE_NAME(Raw file name)=136; Sample descritpion=Sample_colostrum-milk SUBJECT_SAMPLE_FACTORS Human_brest_milk_study 1-BBA_10e_C Sample source:Human breast milk | Lactation_stage:1 | gender:1 RAW_FILE_NAME(Raw file name)=124; Sample descritpion=Sample_colostrum-milk SUBJECT_SAMPLE_FACTORS Human_brest_milk_study 20-BBA_70e_C Sample source:Human breast milk | Lactation_stage:1 | gender:1 RAW_FILE_NAME(Raw file name)=122; Sample descritpion=Sample_colostrum-milk SUBJECT_SAMPLE_FACTORS Human_brest_milk_study 3-BBA_17e_C Sample source:Human breast milk | Lactation_stage:1 | gender:1 RAW_FILE_NAME(Raw file name)=134; Sample descritpion=Sample_colostrum-milk SUBJECT_SAMPLE_FACTORS Human_brest_milk_study 5-BBA_19e_C Sample source:Human breast milk | Lactation_stage:1 | gender:1 RAW_FILE_NAME(Raw file name)=110; Sample descritpion=Sample_colostrum-milk SUBJECT_SAMPLE_FACTORS Human_brest_milk_study 7-BBA_24e_C Sample source:Human breast milk | Lactation_stage:1 | gender:1 RAW_FILE_NAME(Raw file name)=166; Sample descritpion=Sample_colostrum-milk SUBJECT_SAMPLE_FACTORS Human_brest_milk_study 10-BBA_43e_C Sample source:Human breast milk | Lactation_stage:1 | gender:2 RAW_FILE_NAME(Raw file name)=159; Sample descritpion=Sample_colostrum-milk SUBJECT_SAMPLE_FACTORS Human_brest_milk_study 11-BBA_44e_C Sample source:Human breast milk | Lactation_stage:1 | gender:2 RAW_FILE_NAME(Raw file name)=126; Sample descritpion=Sample_colostrum-milk SUBJECT_SAMPLE_FACTORS Human_brest_milk_study 12-BBA_45e_C Sample source:Human breast milk | Lactation_stage:1 | gender:2 RAW_FILE_NAME(Raw file name)=151; Sample descritpion=Sample_colostrum-milk SUBJECT_SAMPLE_FACTORS Human_brest_milk_study 15-BBA_55e_C Sample source:Human breast milk | Lactation_stage:1 | gender:2 RAW_FILE_NAME(Raw file name)=156; Sample descritpion=Sample_colostrum-milk SUBJECT_SAMPLE_FACTORS Human_brest_milk_study 17-BBA_61e_C Sample source:Human breast milk | Lactation_stage:1 | gender:2 RAW_FILE_NAME(Raw file name)=129; Sample descritpion=Sample_colostrum-milk SUBJECT_SAMPLE_FACTORS Human_brest_milk_study 2-BBA_16e_C Sample source:Human breast milk | Lactation_stage:1 | gender:2 RAW_FILE_NAME(Raw file name)=152; Sample descritpion=Sample_colostrum-milk SUBJECT_SAMPLE_FACTORS Human_brest_milk_study 4-BBA_18e_C Sample source:Human breast milk | Lactation_stage:1 | gender:2 RAW_FILE_NAME(Raw file name)=116; Sample descritpion=Sample_colostrum-milk SUBJECT_SAMPLE_FACTORS Human_brest_milk_study 6-BBA_22e_C Sample source:Human breast milk | Lactation_stage:1 | gender:2 RAW_FILE_NAME(Raw file name)=127; Sample descritpion=Sample_colostrum-milk SUBJECT_SAMPLE_FACTORS Human_brest_milk_study 8-BBA_39e_C Sample source:Human breast milk | Lactation_stage:1 | gender:2 RAW_FILE_NAME(Raw file name)=149; Sample descritpion=Sample_colostrum-milk SUBJECT_SAMPLE_FACTORS Human_brest_milk_study 9-BBA_40e_C Sample source:Human breast milk | Lactation_stage:1 | gender:2 RAW_FILE_NAME(Raw file name)=111; Sample descritpion=Sample_colostrum-milk SUBJECT_SAMPLE_FACTORS Human_brest_milk_study 21-BBA_10e_T Sample source:Human breast milk | Lactation_stage:2 | gender:1 RAW_FILE_NAME(Raw file name)=142; Sample descritpion=Sample_transition-milk SUBJECT_SAMPLE_FACTORS Human_brest_milk_study 23-BBA_17e_T Sample source:Human breast milk | Lactation_stage:2 | gender:1 RAW_FILE_NAME(Raw file name)=157; Sample descritpion=Sample_transition-milk SUBJECT_SAMPLE_FACTORS Human_brest_milk_study 25-BBA_19e_T Sample source:Human breast milk | Lactation_stage:2 | gender:1 RAW_FILE_NAME(Raw file name)=162; Sample descritpion=Sample_transition-milk SUBJECT_SAMPLE_FACTORS Human_brest_milk_study 27-BBA_24e_T Sample source:Human breast milk | Lactation_stage:2 | gender:1 RAW_FILE_NAME(Raw file name)=113; Sample descritpion=Sample_transition-milk SUBJECT_SAMPLE_FACTORS Human_brest_milk_study 33-BBA_47e_T Sample source:Human breast milk | Lactation_stage:2 | gender:1 RAW_FILE_NAME(Raw file name)=123; Sample descritpion=Sample_transition-milk SUBJECT_SAMPLE_FACTORS Human_brest_milk_study 34-BBA_48e_T Sample source:Human breast milk | Lactation_stage:2 | gender:1 RAW_FILE_NAME(Raw file name)=108; Sample descritpion=Sample_transition-milk SUBJECT_SAMPLE_FACTORS Human_brest_milk_study 36-BBA_60e_T Sample source:Human breast milk | Lactation_stage:2 | gender:1 RAW_FILE_NAME(Raw file name)=158; Sample descritpion=Sample_transition-milk SUBJECT_SAMPLE_FACTORS Human_brest_milk_study 38-BBA_67e_T Sample source:Human breast milk | Lactation_stage:2 | gender:1 RAW_FILE_NAME(Raw file name)=130; Sample descritpion=Sample_transition-milk SUBJECT_SAMPLE_FACTORS Human_brest_milk_study 39-BBA_69e_T Sample source:Human breast milk | Lactation_stage:2 | gender:1 RAW_FILE_NAME(Raw file name)=103; Sample descritpion=Sample_transition-milk SUBJECT_SAMPLE_FACTORS Human_brest_milk_study 40-BBA_70e_T Sample source:Human breast milk | Lactation_stage:2 | gender:1 RAW_FILE_NAME(Raw file name)=118; Sample descritpion=Sample_transition-milk SUBJECT_SAMPLE_FACTORS Human_brest_milk_study 22-BBA_16e_T Sample source:Human breast milk | Lactation_stage:2 | gender:2 RAW_FILE_NAME(Raw file name)=131; Sample descritpion=Sample_transition-milk SUBJECT_SAMPLE_FACTORS Human_brest_milk_study 24-BBA_18e_T Sample source:Human breast milk | Lactation_stage:2 | gender:2 RAW_FILE_NAME(Raw file name)=128; Sample descritpion=Sample_transition-milk SUBJECT_SAMPLE_FACTORS Human_brest_milk_study 26-BBA_22e_T Sample source:Human breast milk | Lactation_stage:2 | gender:2 RAW_FILE_NAME(Raw file name)=169; Sample descritpion=Sample_transition-milk SUBJECT_SAMPLE_FACTORS Human_brest_milk_study 28-BBA_39e_T Sample source:Human breast milk | Lactation_stage:2 | gender:2 RAW_FILE_NAME(Raw file name)=117; Sample descritpion=Sample_transition-milk SUBJECT_SAMPLE_FACTORS Human_brest_milk_study 29-BBA_40e_T Sample source:Human breast milk | Lactation_stage:2 | gender:2 RAW_FILE_NAME(Raw file name)=119; Sample descritpion=Sample_transition-milk SUBJECT_SAMPLE_FACTORS Human_brest_milk_study 30-BBA_43e_T Sample source:Human breast milk | Lactation_stage:2 | gender:2 RAW_FILE_NAME(Raw file name)=140; Sample descritpion=Sample_transition-milk SUBJECT_SAMPLE_FACTORS Human_brest_milk_study 31-BBA_44e_T Sample source:Human breast milk | Lactation_stage:2 | gender:2 RAW_FILE_NAME(Raw file name)=137; Sample descritpion=Sample_transition-milk SUBJECT_SAMPLE_FACTORS Human_brest_milk_study 32-BBA_45e_T Sample source:Human breast milk | Lactation_stage:2 | gender:2 RAW_FILE_NAME(Raw file name)=164; Sample descritpion=Sample_transition-milk SUBJECT_SAMPLE_FACTORS Human_brest_milk_study 35-BBA_55e_T Sample source:Human breast milk | Lactation_stage:2 | gender:2 RAW_FILE_NAME(Raw file name)=107; Sample descritpion=Sample_transition-milk SUBJECT_SAMPLE_FACTORS Human_brest_milk_study 37-BBA_61e_T Sample source:Human breast milk | Lactation_stage:2 | gender:2 RAW_FILE_NAME(Raw file name)=165; Sample descritpion=Sample_transition-milk SUBJECT_SAMPLE_FACTORS Human_brest_milk_study 41-BBA_10e_M Sample source:Human breast milk | Lactation_stage:3 | gender:1 RAW_FILE_NAME(Raw file name)=145; Sample descritpion=Sample_mature-milk SUBJECT_SAMPLE_FACTORS Human_brest_milk_study 43-BBA_17e_M Sample source:Human breast milk | Lactation_stage:3 | gender:1 RAW_FILE_NAME(Raw file name)=153; Sample descritpion=Sample_mature-milk SUBJECT_SAMPLE_FACTORS Human_brest_milk_study 45-BBA_19e_M Sample source:Human breast milk | Lactation_stage:3 | gender:1 RAW_FILE_NAME(Raw file name)=167; Sample descritpion=Sample_mature-milk SUBJECT_SAMPLE_FACTORS Human_brest_milk_study 47-BBA_24e_M Sample source:Human breast milk | Lactation_stage:3 | gender:1 RAW_FILE_NAME(Raw file name)=161; Sample descritpion=Sample_mature-milk SUBJECT_SAMPLE_FACTORS Human_brest_milk_study 53-BBA_47e_M Sample source:Human breast milk | Lactation_stage:3 | gender:1 RAW_FILE_NAME(Raw file name)=155; Sample descritpion=Sample_mature-milk SUBJECT_SAMPLE_FACTORS Human_brest_milk_study 54-BBA_48e_M Sample source:Human breast milk | Lactation_stage:3 | gender:1 RAW_FILE_NAME(Raw file name)=146; Sample descritpion=Sample_mature-milk SUBJECT_SAMPLE_FACTORS Human_brest_milk_study 56-BBA_60e_M Sample source:Human breast milk | Lactation_stage:3 | gender:1 RAW_FILE_NAME(Raw file name)=135; Sample descritpion=Sample_mature-milk SUBJECT_SAMPLE_FACTORS Human_brest_milk_study 58-BBA_67e_M Sample source:Human breast milk | Lactation_stage:3 | gender:1 RAW_FILE_NAME(Raw file name)=115; Sample descritpion=Sample_mature-milk SUBJECT_SAMPLE_FACTORS Human_brest_milk_study 59-BBA_69e_M Sample source:Human breast milk | Lactation_stage:3 | gender:1 RAW_FILE_NAME(Raw file name)=109; Sample descritpion=Sample_mature-milk SUBJECT_SAMPLE_FACTORS Human_brest_milk_study 60-BBA_70e_M Sample source:Human breast milk | Lactation_stage:3 | gender:1 RAW_FILE_NAME(Raw file name)=114; Sample descritpion=Sample_mature-milk SUBJECT_SAMPLE_FACTORS Human_brest_milk_study 42-BBA_16e_M Sample source:Human breast milk | Lactation_stage:3 | gender:2 RAW_FILE_NAME(Raw file name)=138; Sample descritpion=Sample_mature-milk SUBJECT_SAMPLE_FACTORS Human_brest_milk_study 44-BBA_18e_M Sample source:Human breast milk | Lactation_stage:3 | gender:2 RAW_FILE_NAME(Raw file name)=170; Sample descritpion=Sample_mature-milk SUBJECT_SAMPLE_FACTORS Human_brest_milk_study 46-BBA_22e_M Sample source:Human breast milk | Lactation_stage:3 | gender:2 RAW_FILE_NAME(Raw file name)=121; Sample descritpion=Sample_mature-milk SUBJECT_SAMPLE_FACTORS Human_brest_milk_study 48-BBA_39e_M Sample source:Human breast milk | Lactation_stage:3 | gender:2 RAW_FILE_NAME(Raw file name)=105; Sample descritpion=Sample_mature-milk SUBJECT_SAMPLE_FACTORS Human_brest_milk_study 49-BBA_40e_M Sample source:Human breast milk | Lactation_stage:3 | gender:2 RAW_FILE_NAME(Raw file name)=120; Sample descritpion=Sample_mature-milk SUBJECT_SAMPLE_FACTORS Human_brest_milk_study 50-BBA_43e_M Sample source:Human breast milk | Lactation_stage:3 | gender:2 RAW_FILE_NAME(Raw file name)=104; Sample descritpion=Sample_mature-milk SUBJECT_SAMPLE_FACTORS Human_brest_milk_study 51-BBA_44e_M Sample source:Human breast milk | Lactation_stage:3 | gender:2 RAW_FILE_NAME(Raw file name)=144; Sample descritpion=Sample_mature-milk SUBJECT_SAMPLE_FACTORS Human_brest_milk_study 52-BBA_45e_M Sample source:Human breast milk | Lactation_stage:3 | gender:2 RAW_FILE_NAME(Raw file name)=147; Sample descritpion=Sample_mature-milk SUBJECT_SAMPLE_FACTORS Human_brest_milk_study 55-BBA_55e_M Sample source:Human breast milk | Lactation_stage:3 | gender:2 RAW_FILE_NAME(Raw file name)=143; Sample descritpion=Sample_mature-milk SUBJECT_SAMPLE_FACTORS Human_brest_milk_study 57-BBA_61e_M Sample source:Human breast milk | Lactation_stage:3 | gender:2 RAW_FILE_NAME(Raw file name)=106; Sample descritpion=Sample_mature-milk SUBJECT_SAMPLE_FACTORS Human_brest_milk_study 66-Extr_Blank1 Sample source:n.a. | Lactation_stage:0 | gender:0 RAW_FILE_NAME(Raw file name)=102; Sample descritpion=Extraction blank SUBJECT_SAMPLE_FACTORS Human_brest_milk_study 67-Extr_Blank2 Sample source:n.a. | Lactation_stage:0 | gender:0 RAW_FILE_NAME(Raw file name)=171; Sample descritpion=Extraction blank SUBJECT_SAMPLE_FACTORS Human_brest_milk_study Buffer phosphate blank Sample source:n.a. | Lactation_stage:0 | gender:0 RAW_FILE_NAME(Raw file name)=101; Sample descritpion=Buffer blank #COLLECTION CO:COLLECTION_SUMMARY 7.5 to 20 mL of milk samples were collected from mothers and kept in sterile CO:COLLECTION_SUMMARY 50-mL tubes (Sigma-Aldrich). The samples were kept cold and transported to the CO:COLLECTION_SUMMARY laboratory within the first two hours of the sampling. 5 mL of the breast milk CO:COLLECTION_SUMMARY samples were aliquoted in cryovials and stored at -80oC for glutamate analysis. CO:COLLECTION_SUMMARY Lactation stage 1 = colostrum, 2 = transition, 3 = mature. CO:SAMPLE_TYPE Milk #TREATMENT TR:TREATMENT_SUMMARY Mothers were recruited at Hospital Gineco Obstétrico Isidro Ayora in Quito, TR:TREATMENT_SUMMARY Ecuador. The participating mothers were permanent residents and indicated their TR:TREATMENT_SUMMARY willingness to exclusively breastfeed for at least two months. Mothers were TR:TREATMENT_SUMMARY healthy, ≥18 years of age, and primiparous, and they had not consumed TR:TREATMENT_SUMMARY antibiotics or probiotics during pregnancy. Each mother had given birth to a TR:TREATMENT_SUMMARY full-term baby via vaginal delivery. A total of 20 mothers donated their TR:TREATMENT_SUMMARY colostrum- (human milk from 1-7 days postpartum), transition- (15 days TR:TREATMENT_SUMMARY postpartum), and mature-milk (30 days postpartum). For Factor_2 (gender) in TR:TREATMENT_SUMMARY Study Design, 1: participant with a female baby; 2: one with a male baby; 0: not TR:TREATMENT_SUMMARY applicable. #SAMPLEPREP SP:SAMPLEPREP_SUMMARY Human breast milk samples were extracted using a two-phase extraction method. 2 SP:SAMPLEPREP_SUMMARY mL of chloroform-methanol (2:1) was added to each breast milk sample and SP:SAMPLEPREP_SUMMARY vortexed for 10 seconds. 600 µL of water was then added to each sample and the SP:SAMPLEPREP_SUMMARY mixture was centrifuged. 800 µL of the polar phase from each sample was SP:SAMPLEPREP_SUMMARY transferred into a 1.5mL glass tube. The samples were then dried using a speed SP:SAMPLEPREP_SUMMARY vacuum centrifuge. The polar phase was reconstituted in 700 µL of a phosphate SP:SAMPLEPREP_SUMMARY buffer (pH 7.4; 1M TSP). The samples were transferred to 5-mm NMR tubes for NMR SP:SAMPLEPREP_SUMMARY analysis. SP:SAMPLEPREP_PROTOCOL_FILENAME 4_Sample_preparation_protocol_Glu_2024.pdf SP:PROCESSING_STORAGE_CONDITIONS 4℃ #CHROMATOGRAPHY #ANALYSIS AN:ANALYSIS_TYPE NMR AN:ANALYSIS_PROTOCOL_FILE 5_Analysis_protocol_Glu_2024.pdf #NMR NM:INSTRUMENT_NAME Bruker AVANCE lll NM:INSTRUMENT_TYPE FT-NMR NM:NMR_EXPERIMENT_TYPE 2D-1H-1H NM:NMR_COMMENTS The raw NMR data files are in the 'Spectra' folder. NM:SPECTROMETER_FREQUENCY 600 #NMR_METABOLITE_DATA NMR_METABOLITE_DATA:UNITS NMR peak intensity NMR_METABOLITE_DATA_START Samples 13-BBA_47e_C 14-BBA_48e_C 16-BBA_60e_C 18-BBA_67e_C 19-BBA_69e_C 1-BBA_10e_C 20-BBA_70e_C 3-BBA_17e_C 5-BBA_19e_C 7-BBA_24e_C 10-BBA_43e_C 11-BBA_44e_C 12-BBA_45e_C 15-BBA_55e_C 17-BBA_61e_C 2-BBA_16e_C 4-BBA_18e_C 6-BBA_22e_C 8-BBA_39e_C 9-BBA_40e_C 21-BBA_10e_T 23-BBA_17e_T 25-BBA_19e_T 27-BBA_24e_T 33-BBA_47e_T 34-BBA_48e_T 36-BBA_60e_T 38-BBA_67e_T 39-BBA_69e_T 40-BBA_70e_T 22-BBA_16e_T 24-BBA_18e_T 26-BBA_22e_T 28-BBA_39e_T 29-BBA_40e_T 30-BBA_43e_T 31-BBA_44e_T 32-BBA_45e_T 35-BBA_55e_T 37-BBA_61e_T 41-BBA_10e_M 43-BBA_17e_M 45-BBA_19e_M 47-BBA_24e_M 53-BBA_47e_M 54-BBA_48e_M 56-BBA_60e_M 58-BBA_67e_M 59-BBA_69e_M 60-BBA_70e_M 42-BBA_16e_M 44-BBA_18e_M 46-BBA_22e_M 48-BBA_39e_M 49-BBA_40e_M 50-BBA_43e_M 51-BBA_44e_M 52-BBA_45e_M 55-BBA_55e_M 57-BBA_61e_M Factors Sample source:Human breast milk | Lactation_stage:1 | gender:1 Sample source:Human breast milk | Lactation_stage:1 | gender:1 Sample source:Human breast milk | Lactation_stage:1 | gender:1 Sample source:Human breast milk | Lactation_stage:1 | gender:1 Sample source:Human breast milk | Lactation_stage:1 | gender:1 Sample source:Human breast milk | Lactation_stage:1 | gender:1 Sample source:Human breast milk | Lactation_stage:1 | gender:1 Sample source:Human breast milk | Lactation_stage:1 | gender:1 Sample source:Human breast milk | Lactation_stage:1 | gender:1 Sample source:Human breast milk | Lactation_stage:1 | gender:1 Sample source:Human breast milk | Lactation_stage:1 | gender:2 Sample source:Human breast milk | Lactation_stage:1 | gender:2 Sample source:Human breast milk | Lactation_stage:1 | gender:2 Sample source:Human breast milk | Lactation_stage:1 | gender:2 Sample source:Human breast milk | Lactation_stage:1 | gender:2 Sample source:Human breast milk | Lactation_stage:1 | gender:2 Sample source:Human breast milk | Lactation_stage:1 | gender:2 Sample source:Human breast milk | Lactation_stage:1 | gender:2 Sample source:Human breast milk | Lactation_stage:1 | gender:2 Sample source:Human breast milk | Lactation_stage:1 | gender:2 Sample source:Human breast milk | Lactation_stage:2 | gender:1 Sample source:Human breast milk | Lactation_stage:2 | gender:1 Sample source:Human breast milk | Lactation_stage:2 | gender:1 Sample source:Human breast milk | Lactation_stage:2 | gender:1 Sample source:Human breast milk | Lactation_stage:2 | gender:1 Sample source:Human breast milk | Lactation_stage:2 | gender:1 Sample source:Human breast milk | Lactation_stage:2 | gender:1 Sample source:Human breast milk | Lactation_stage:2 | gender:1 Sample source:Human breast milk | Lactation_stage:2 | gender:1 Sample source:Human breast milk | Lactation_stage:2 | gender:1 Sample source:Human breast milk | Lactation_stage:2 | gender:2 Sample source:Human breast milk | Lactation_stage:2 | gender:2 Sample source:Human breast milk | Lactation_stage:2 | gender:2 Sample source:Human breast milk | Lactation_stage:2 | gender:2 Sample source:Human breast milk | Lactation_stage:2 | gender:2 Sample source:Human breast milk | Lactation_stage:2 | gender:2 Sample source:Human breast milk | Lactation_stage:2 | gender:2 Sample source:Human breast milk | Lactation_stage:2 | gender:2 Sample source:Human breast milk | Lactation_stage:2 | gender:2 Sample source:Human breast milk | Lactation_stage:2 | gender:2 Sample source:Human breast milk | Lactation_stage:3 | gender:1 Sample source:Human breast milk | Lactation_stage:3 | gender:1 Sample source:Human breast milk | Lactation_stage:3 | gender:1 Sample source:Human breast milk | Lactation_stage:3 | gender:1 Sample source:Human breast milk | Lactation_stage:3 | gender:1 Sample source:Human breast milk | Lactation_stage:3 | gender:1 Sample source:Human breast milk | Lactation_stage:3 | gender:1 Sample source:Human breast milk | Lactation_stage:3 | gender:1 Sample source:Human breast milk | Lactation_stage:3 | gender:1 Sample source:Human breast milk | Lactation_stage:3 | gender:1 Sample source:Human breast milk | Lactation_stage:3 | gender:2 Sample source:Human breast milk | Lactation_stage:3 | gender:2 Sample source:Human breast milk | Lactation_stage:3 | gender:2 Sample source:Human breast milk | Lactation_stage:3 | gender:2 Sample source:Human breast milk | Lactation_stage:3 | gender:2 Sample source:Human breast milk | Lactation_stage:3 | gender:2 Sample source:Human breast milk | Lactation_stage:3 | gender:2 Sample source:Human breast milk | Lactation_stage:3 | gender:2 Sample source:Human breast milk | Lactation_stage:3 | gender:2 Sample source:Human breast milk | Lactation_stage:3 | gender:2 Glutamate 0.0075 0.0100 0.0039 0.0052 0.0049 0.0035 0.0071 0.0026 0.0079 0.0109 0.0051 0.0049 0.0054 0.0088 0.0136 0.0036 0.0070 0.0910 0.0072 0.0163 0.0042 0.0096 0.0165 0.0141 0.0108 0.0132 0.0058 0.0136 0.0194 0.0081 0.0048 0.0106 0.0085 0.0138 0.0068 0.0154 0.0173 0.0185 0.0094 0.0130 0.0037 0.0109 0.0174 0.0085 0.0182 0.0146 0.0157 0.0248 0.0155 0.0130 0.0121 0.0125 0.0122 0.0186 0.0126 0.0182 0.0194 0.0155 0.0185 0.0122 NMR_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name pubchem_id inchi_key kegg_id other_id other_id_type ri ri_type moverz_quant Glutamate 4525487 METABOLITES_END #END